CN109997622A - The method for improving dendrobium candidum active constituent content - Google Patents
The method for improving dendrobium candidum active constituent content Download PDFInfo
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- CN109997622A CN109997622A CN201910306754.2A CN201910306754A CN109997622A CN 109997622 A CN109997622 A CN 109997622A CN 201910306754 A CN201910306754 A CN 201910306754A CN 109997622 A CN109997622 A CN 109997622A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The present invention relates to a kind of methods for improving dendrobium candidum active constituent content.The method of the present invention carries out light filling processing using blue light or red blue complex light by the dendrobium candidum to picking time.The method of the present invention improves the content of the effective components such as dendrobium candidum alkaloid, polysaccharide, polyphenol content, flavonoids.In addition, the present invention carries out light filling processing to the dendrobium nobile of picking time, damage of the long-term light benefit to dendrobium candidum seedling is avoided, to improve the survival rate and biomass of dendrobium candidum.Meanwhile the present invention only needs to carry out light filling in picking time, the time needed for can also greatly shortening light filling processing, reduces economic cost and cost of labor.
Description
Technical field
The present invention relates to field of plant cultivation, more particularly to a kind of method for improving dendrobium candidum active constituent content.
Background technique
Dendrobium candidum is China's tradition rare traditional Chinese medicine first of nine big mesonas, and Chinese Second Class Key Protected Plant has enriching yin
Heat-clearing, reinforcing stomach reg fluid, moistening lung improving eyesight, the anti-effect of waiting for a long time of anticancer, therefore obtain more people's favors.But dendrobium officinale
It is endangered due to excessively excavating and ecological disruption.1987 wild in " Key Protected medicinal material species catalogue "
Raw dendrobium candidum is listed in focused protection species, and the market price of product is more and more expensive.Since the new century, with cultivation
The breakthrough of technology, dendrobium candidum industry have obtained quick development, and the existing cultivated area in the whole nation breaks through 2000hm2, output value breakthrough
3000000000 yuan, become one of China's volume of production and marketing maximum, Chinese medicine with fastest developing speed.
Why dendrobium candidum has nourishing Yin and clearing heat, reinforcing stomach reg fluid, moistening lung improving eyesight, the anti-effect of waiting for a long time of anticancer, is because of its body
It is interior to contain these nutritive and health protection components such as dendrobium polysaccharide, alkaloid, flavonoids, polyphenol.Studies have shown that polysaccharide component and iron sheet stone
The drug effect of dry measure used in former times such as enhances human body immune function, antitumor, anti-aging, antifatigue, hypoglycemic etc., there is close connection.
The pharmacological action of alkaloid is mainly showed to cardiovascular, gastrointestinal tract effect and antipyretic analgesic etc.；Polyphenol, flavonoids, luxuriant and rich with fragrance class etc. at
Dividing has Pycnorus cinnabarius.
Therefore, the drug effect or quality of Yao Tigao dendrobium candidum is exactly to improve its effective component at all.Mainly pass through at present
The methods of nutrient solution, UV-B processing are improved to improve the effective component of dendrobium candidum.But nutrient solution is only had studied at present to stone
The influence of dry measure used in former times polyoses content, single effect are not comprehensive enough.In existing research, the research of light quality regulation is only limited to dendrobium candidum
Tissue-cultured seedling handled, process is complicated, and time cost and human cost are high, and be easy to cause that plant is sallow, falls leaf phenomenon,
Largely effect on yield.
Summary of the invention
Based on this, the present invention provides a kind of method for improving dendrobium candidum active constituent content, and this method is to picking time
Dendrobium candidum carries out light filling processing, and containing for the effective components such as dendrobium candidum alkaloid, polysaccharide, polyphenol content, flavonoids can be improved
Amount.
Specific technical solution is as follows:
The present invention provides a kind of method for improving dendrobium candidum active constituent content, uses to the dendrobium candidum of picking time
Blue light or red blue complex light carry out light filling processing.
Compared with prior art, the invention has the following advantages:
The present inventor creatively selects to carry out the dendrobium candidum of picking time using blue light or red blue complex light
Light filling processing, can promote the accumulation of plant assimilating product and antioxidant, improve the metabolic capability of dendrobium candidum and resist
Oxidability, to improve the content of the effective components such as dendrobium candidum alkaloid, polysaccharide, polyphenol content, flavonoids significantly.
In addition, the present invention carries out light filling processing to the dendrobium nobile of picking time, damage of the long-term light filling to dendrobium candidum seedling is avoided, thus
Improve the survival rate and biomass of dendrobium candidum.Meanwhile the method for the present invention only needs to carry out light filling in picking time, it can also be significantly
Time needed for shortening light filling processing, reduce economic cost and cost of labor.
Detailed description of the invention
Fig. 1 is the Technology Roadmap of the embodiment of the present invention 1；
Fig. 2 is the test scene figure of the embodiment of the present invention 1；
Fig. 3 is influence of the different illumination to dendrobium candidum alkaloid；
Fig. 4 is influence of the different illumination to polysaccharide content of dendrobium candidum；
Fig. 5 is influence of the different illumination to dendrobium candidum Flavonoid Content；
Fig. 6 is influence of the different illumination to dendrobium candidum polyphenol content.
Specific embodiment
To facilitate the understanding of the present invention, it below with reference to embodiment to invention is more fully described, is given below
Presently preferred embodiments of the present invention.But the invention can be realized in many different forms, however it is not limited to described herein
Embodiment.Purpose of providing these embodiments is makes the disclosure of the present invention more thorough and comprehensive.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Used term is intended merely to describe specific reality in the description of the invention
Apply the purpose of example, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more relevant institutes
Any and all combinations of list of items.
The present invention provides a kind of method for improving dendrobium candidum active constituent content, uses to the dendrobium candidum of picking time
Blue light or red blue complex light carry out light filling processing.
The picking time of dendrobium candidum of the invention refers to: before blooming or dormant period is harvested (next year 3 in November-
Month).Harvesting is with the next winter after dendrobe cultivation, and the blade on branch senesces flavescences, and part stem strain, which starts to fall leaves, is
Standard.
The wavelength of feux rouges in the red blue complex light is preferably 660 ± 10nm；Blue light in the red blue complex light
Wavelength is preferably 460 ± 10nm.Preferably, the wavelength of the blue light is 460nm；The wavelength of the feux rouges is 660nm.
The light filling processing refers to carrying out supplementary light with dendrobium candidum of the above-mentioned light to picking time.It can specifically use
The LED light of light is stated as light source, lamp plate is assembled into and carries out supplementary light.
The light filling processing of this method refers to carrying out supplementary light in daylight weaker time, the daylight it is weaker when
Between can for before sunrise, after the sunset, cloudy day or the rainy day.Carrying out light filling processing in above-mentioned time interval can be with the sunshine on daytime
It cooperates, most effectively significantly improves the content of effective component in dendrobium candidum in a short time.
This method only needs to carry out light filling using blue light or red blue complex light in picking time, easy to operate, can promote to plant
The accumulation of object assimilation products and antioxidant improves the metabolic capability and oxidation resistance of dendrobium candidum, is conducive to picking time
Synthesis, conversion and the accumulation of dendrobium candidum effective component improve picking time dendrobium candidum alkaloid, polysaccharide, polyphenol in which can dramatically
The content of the effective components such as content and flavonoids.
The further preferred intensity of illumination of blue light described in this method or red blue complex light is (50-150) μm olm-2·
s-1.Under the intensity of illumination range, the photosynthetic efficiency of dendrobium candidum is more excellent.
This method preferably carries out light filling processing using red blue complex light to the dendrobium candidum of picking time.Different light qualities pair
The influence degree of the content of the effective component of dendrobium candidum is different, Dendrobium officinale polysaccharide, class can be made yellow using red blue complex light
The content of the plurality of active ingredients such as ketone, polyphenol, alkaloid is remarkably improved.
The intensity of illumination of red indigo plant complex light is preferably (100 ± 10) μm olm-2·s-1.Under the intensity of illumination range,
It is more significant to the raising of the content of dendrobium candidum effective component.
When this method carries out light filling processing using red blue complex light to the dendrobium nobile of picking time, in red indigo plant complex light feux rouges with
The content that the intensity of illumination of blue light compares dendrobium candidum effective component has different influences.In the present invention, feux rouges and blue light
Intensity of illumination than be preferably (6-8): (2-4).It is further preferred that the illumination of feux rouges and blue light is strong in the red blue complex light
Degree is than being 7:3.The intensity of illumination of feux rouges and blue light ratio is in this range in hot indigo plant complex light, to dendrobium candidum effective component
The improvement effect of content is more preferably.Intensity of illumination further combined with red blue complex light is (100 ± 10) μm olm-2·s-1, can
To further increase effect.
When dendrobium nobile of this method to picking time carries out light filling processing using blue light, the intensity of illumination of the blue light is 60-100
μmol·m-2·s-1.The intensity of illumination of blue light can preferably improve the content of dendrobium candidum effective component in the range.Tool
Body, the intensity of illumination of the blue light is 60 μm of olm-2·s-1、70μmol·m-2·s-1、80μmol·m-2·s-1、90μ
mol·m-2·s-1Or 100 μm of olm-2·s-1。
The periodicity of illumination of this method light filling processing is 12-20 days, the when a length of 10-14h that daily light filling is handled.More preferably
Ground, the periodicity of illumination of light filling processing are 15 days, the when a length of 12h of daily light filling processing.When light filling processing is in this range, light filling
It is high to the raising efficiency of dendrobium candidum active constituent content.
The time interval of light filling processing can be 19:00- next day 9:00；The further preferably described daily light filling processing when
Between section be 20:00- next day 8:00.
In wherein some embodiments, harvest within 0-30 days after light filling processing.It is preferred that being harvested immediately after light filling processing, at light filling
Harvesting in 1-30 days influences the content of effective component unobvious after reason.
Below in conjunction with specific embodiment, the present invention is described in further detail.
The wavelength of feux rouges used in the embodiment of the present invention is 660 ± 10nm, and the wavelength of blue light is 460 ± 10nm.
Embodiment 1
Select 40 plants of dendrobium candidum in picking time.Light filling is carried out to dendrobium candidum using the compound lamp plate of the red blue light of LED
Processing, the when a length of 12h of illumination, the time of illumination are 20:00-next day 8:00, and intensity of illumination is 100 μm of olm-2·s-1
(wherein red 70 μm of olm-2·s-130 μm of olm of+indigo plant-2·s-1), light filling harvests after handling 15 days.Measure every plant of dendrobium candidum
The content of middle effective component, takes mean value.Technology path tests scene referring to attached drawing 2 referring to attached drawing 1.
Embodiment 2
Select 40 plants of dendrobium candidum in picking time.Light filling processing, light are carried out to dendrobium candidum using LED blue light board
According to when a length of 12h, the time of illumination is 20:00-next day 8:00, and intensity of illumination is 60 μm of olm-2·s-1, light filling processing
It is harvested after 15 days.The content for measuring effective component in every plant of dendrobium candidum, takes mean value.
Embodiment 3
Select 40 plants of dendrobium candidum in picking time.Light filling processing, light are carried out to dendrobium candidum using LED blue light board
According to when a length of 12h, the time of illumination is 20:00-next day 8:00, and intensity of illumination is 80 μm of olm-2·s-1, light filling processing
It is harvested after 15 days.The content for measuring effective component in every plant of dendrobium candidum, takes mean value.
Embodiment 4
Select 40 plants of dendrobium candidum in picking time.Light filling processing, light are carried out to dendrobium candidum using LED blue light board
According to when a length of 12h, the time of illumination is 20:00-next day 8:00, and intensity of illumination is 100 μm of olm-2·s-1, light filling processing
It is harvested after 15 days.The content for measuring effective component in every plant of dendrobium candidum, takes mean value.
Embodiment 5
Select 40 plants of dendrobium candidum in picking time.Light filling is carried out to dendrobium candidum using the compound lamp plate of the red blue light of LED
Processing, the when a length of 12h of illumination, the time of illumination are 20:00-next day 8:00, and intensity of illumination is 100 μm of olm-2·s-1
(wherein red 50 μm of olm-2·s-150 μm of olm of+indigo plant-2·s-1), light filling harvests after handling 15 days.Measure every plant of dendrobium candidum
The content of middle effective component, takes mean value.
Embodiment 6
Select 40 plants of dendrobium candidum in picking time.Light filling is carried out to dendrobium candidum using the compound lamp plate of the red blue light of LED
Processing, the when a length of 12h of illumination, the time of illumination are 20:00-next day 8:00, and intensity of illumination is 100 μm of olm-2·s-1
(wherein red 30 μm of olm-2·s-170 μm of olm of+indigo plant-2·s-1), light filling harvests after handling 15 days.Measure every plant of dendrobium candidum
The content of middle effective component, takes mean value.
Embodiment 7
Select 40 plants of dendrobium candidum in picking time.Light filling is carried out to dendrobium candidum using the compound lamp plate of the red blue light of LED
Processing, the when a length of 14h of illumination, the time of illumination are 19:00-next day 9:00, and intensity of illumination is 100 μm of olm-2·s-1
(wherein red 70 μm of olm-2·s-130 μm of olm of+indigo plant-2·s-1), light filling harvests after handling 15 days.Measure every plant of dendrobium candidum
The content of middle effective component, takes mean value.
Embodiment 8
Select 40 plants of dendrobium candidum in picking time.Light filling is carried out to dendrobium candidum using the compound lamp plate of the red blue light of LED
Processing, the when a length of 10h of illumination, the time of illumination are 21:00-next day 7:00, and intensity of illumination is 100 μm of olm-2·s-1
(wherein red 70 μm of olm-2·s-130 μm of olm of+indigo plant-2·s-1), light filling harvests after handling 15 days.Measure every plant of dendrobium candidum
The content of middle effective component, takes mean value.
Using phenol Sulphuric acid colorimetry[1]Measure the dendrobium candidum of embodiment 1-8, control group (dendrobium candidum of non-light filling)
The content of middle polysaccharide；
Using ferrous tartrate method[2]Measure the content of polyphenol in dendrobium candidum；
Using aluminum nitrate colorimetric method[3]Measure the content of flavonoids in dendrobium candidum；
Using acid-dye colorimetry[4]Measure the content of alkaloid in dendrobium candidum.
[1] Zhao Wenchang, Song Lijun, Xu Jianhuang natural polymer bletilla polysaccharide in medicine preparation application [J] today
Pharmacy, 2010,20 (3): 2-3.
[2] Wang Lizhu, Wu Lingyao, primitive root wait Spectrophotometric Determination of Tea-Polyphenol with Ferrous Tartrate [J] spectrum experiment
Room, 1997,14 (3): 52-54.
[3] Li Hansheng, Yao Deheng, Chen Xiaohui, influence [J] heat for waiting blue light to be metabolized longan cell culture and flavonoids
Band Acta Agronomica Sinica, 2018,39 (4): 694-701.
[4] in Lan Song Wan withAcid-dye Colorimetry Cortex Phellodendri total alkaloid the Guangdong content [J] chemical industry, 2018,45
(24): 37-38.
Measurement result is as shown in table 1 and attached drawing 3-6.
Influence of 1 light quality of table to dendrobium candidum picking time active constituent content
From the figure 3, it may be seen that the present invention is improved using blue light or the dendrobium candidum of red indigo plant compound light processing picking time significantly
The content of dendrobium candidum alkaloid.In embodiment 1-4, successively are as follows: red indigo plant complex light 100 (intensity) (red 70+ indigo plant 30) > blue
60 > indigo plant 100 > indigo plant 80 > control, each processing improve 71.64%, 46.86%, 39.40%, 27.16% than control respectively, and right
According to group compared to significant difference.It analyzes from data it is found that red 70 μm of olm-2·s-130 μm of olm of+indigo plant-2·s-1It is red
Blue complex light light filling 12h is the optimal processing for improving dendrobium candidum alkaloid.
As shown in Figure 4, compared with the control, embodiment 1, embodiment 2, embodiment 3 have been significantly increased Dendrobium officinale polysaccharide
Content, respectively than control improve 55.91%, 16.31%, 12.58%, compared with the control group have significant difference.It is red
70μmol·m-2·s-130 μm of olm of+indigo plant-2·s-1The red compound light processing of indigo plant under dendrobium polysaccharide content improve it is most significant.
As shown in Figure 5, with compare, the red compound light processing of indigo plant of embodiment 1 significantly improves dendrobium candidum flavonoids
Content improves 24.86% than control.Embodiment 3 (indigo plant 80) improves 6.63% than control group.
It will be appreciated from fig. 6 that compared with the control, the present invention is equal using blue light or the dendrobium candidum of red indigo plant compound light processing picking time
The content of the polyphenol of dendrobium candidum is improved significantly.In embodiment 1-4, be successively: red indigo plant complex light 100 (intensity) is (red
70+ indigo plant 30) > indigo plant 80 > indigo plant 100 > indigo plant 60 > control, 22.57%, 17.06%, 9.45%, 8.40% is improved than control respectively.
It analyzes from data it is found that red 70 μm of olm-2·s-130 μm of olm of+indigo plant-2·s-1Red indigo plant compound light processing light filling 12h when iron
The polyphenol content highest of skin dendrobium nobile.
In conclusion the present invention uses blue light or the dendrobium candidum of red indigo plant compound light processing picking time, iron sheet stone is improved
The content of the effective components such as dry measure used in former times alkaloid, polysaccharide, polyphenol content and flavonoids.And the survival rate of light filling treated dendrobium candidum
It is 100%, the growth of dendrobium candidum is had no significant effect.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of method for improving dendrobium candidum active constituent content, which is characterized in that used to the dendrobium candidum of picking time blue
Light or red blue complex light carry out light filling processing.
2. the method according to claim 1, wherein the intensity of illumination of the blue light or red blue complex light is (50-
150)μmol·m-2·s-1。
3. according to the method described in claim 2, it is characterized in that, the dendrobium candidum to picking time is carried out using red blue complex light
The intensity of illumination of light filling processing, the red blue complex light is (100 ± 10) μm olm-2·s-1。
4. according to the method described in claim 3, it is characterized in that, in the red blue complex light feux rouges and blue light intensity of illumination
Than for (6-8): (2-4).
5. according to the method described in claim 4, it is characterized in that, in the red blue complex light feux rouges and blue light intensity of illumination
Than for 7:3.
6. the method according to claim 1, wherein carry out light filling processing using blue light to the dendrobium nobile of picking time,
The intensity of illumination of the blue light is 60-100 μm of olm-2·s-1。
7. method according to claim 1-6, which is characterized in that the wavelength of the blue light is 460 ± 10nm；Institute
The wavelength for stating feux rouges is 660 ± 10nm.
8. method according to claim 1-6, which is characterized in that the periodicity of illumination of the light filling processing is 12-
20 days, the when a length of 10-14h of daily light filling processing.
9. according to the method described in claim 8, it is characterized in that, the light filling processing periodicity of illumination be 15 days, daily mend
The when a length of 12h of light processing.
10. according to the method described in claim 9, it is characterized in that, the time interval of the daily light filling processing is 20:00-
Next day 8:00.
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| CN114402837A (en) * | 2022-01-12 | 2022-04-29 | 中国科学院合肥物质科学研究院 | Method for improving content of effective components in eucommia ulmoides leaves by utilizing LED red and blue illumination |
| CN115777375A (en) * | 2022-12-21 | 2023-03-14 | 广东省农业科学院环境园艺研究所 | UV-A radiation method for increasing content of active substances in dendrobium |
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