CN103704035B - A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component - Google Patents
A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component Download PDFInfo
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- CN103704035B CN103704035B CN201310734851.4A CN201310734851A CN103704035B CN 103704035 B CN103704035 B CN 103704035B CN 201310734851 A CN201310734851 A CN 201310734851A CN 103704035 B CN103704035 B CN 103704035B
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Abstract
The invention discloses a kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component, comprise and in the course of cultivation UVB radiation is manually increased to dendrobium candidum, the content of polysaccharose and phenols component in dendrobium candidum stem can be made to improve 23.1 ~ 55.6% and 9.8 ~ 65.0% respectively.Cultivation method of the present invention improves the content of active component polysaccharides class and phenols component in dendrobium candidum effectively, improves the quality of dendrobium candidum, has very important medical value and economic worth.And this method is simply easy to operation, is suitable for the requirement of the large production of dendrobium candidum scale.
Description
Technical field
The invention belongs to field of plant cultivation, be specifically related to a kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component.
Background technology
Dendrobium candidum
dendrobium officinalekimura et Migo is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, because its eustipes part is pitchy, has another name called ribbed hedyotis herb.Its fresh or dry stem uses as medicinal material, and be rare crude drugs, medicinal history is long, is classified as top grade in Shennong's Herbal, has nourishing Yin and clearing heat, and promote the production of body fluid beneficial stomach, and the effect such as moisten the lung and relieve the cough.The dendrobium candidum stem of noble dendrobium requires harsher to growing environment, needs special ecological environmental condition, and dendrobium candidum stem of noble dendrobium wild resource is few, within 1987, is classified as one of animals and plants medicinal material of dying out in imminent danger by country.
Main active in dendrobium candidum is polysaccharose wherein and phenols component.Polysaccharose composition is the active component (Li Manfei etc. in dendrobium candidum with immunological enhancement and antitumor action, Chinese herbal medicine, 1990,21 (10): 10), and content higher (yellow civil rights etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1997,22(2): 74), the content of " Chinese Pharmacopoeia " 2010 editions regulation Dendrobium officinale polysaccharides must not be less than 25%.Phenols component is a compound class phenyl ring with hydroxyl, phenols component in dendrobium candidum mainly stilbene compound, (Malaysian is auspicious to comprise two benzyl phenols and phenanthrol compounds EN and chrysotoxene, phenanthrenequione parent nucleus HERBA DENDROBII phenanthrenequione, Bibenzyl compound and dendrophnol etc., China Medicine University's journal, 1994,25 (3): 188 ~ 189; Li Yan etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2010,35 (13): 1715-1719), its pharmacologically active is mainly antitumor.In addition, alkaloids composition (0.02% ~ 0.04%) containing trace in dendrobium candidum, but the content of alkaloids composition is only 1/20 of HERBA DENDROBII in dendrobium candidum, alkaloid is not the main component (Chen Xiaomei etc. of dendrobium candidum, Chinese Academy of Medical Sciences's journal, 2006,28 (4): 524 ~ 529; Zhu Yan etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2010,35 (18): 2388-2390).Thus, the quality of how many direct reflection dendrobium candidum qualities of polysaccharose composition and phenols component in dendrobium candidum, the content being improved polysaccharose and phenols component in dendrobium candidum by cultivation management method is had great importance for the dendrobium candidum of production high-quality.
Summary of the invention
The object of the present invention is to provide a kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component.
The technical solution used in the present invention is:
Improve a cultivation method for polysaccharose and phenols component in dendrobium candidum, comprise and in the course of cultivation UVB radiation is manually increased to dendrobium candidum, the content of polysaccharose and phenols component in dendrobium candidum stem can be made to improve 23.1 ~ 55.6% and 9.8 ~ 65.0% respectively.
Described UVB irradiation refers to that any stage in officinal dendrobium stem plantation process implements UVB and irradiates more than 1 month.
As preferably, described UVB irradiation refers to and to carry out in 3 months before dendrobium candidum is gathered, and every day is irradiation more than 1 hour at least.
UVB irradiation intensity is 0.35 ~ 30 μ W.cm
-2.
In UVB irradiation process, take seedbed to spray, keep the humidity of dendrobium officinale culture medium more than 70%, temperature, between 25 ~ 35 DEG C, to eliminate the adverse effects such as fuel factor that UVB irradiation brings, improves the resistance of dendrobium candidum.
Described officinal dendrobium stem plantation is in booth, and booth, with bluish purple plastic film covering, in order to other illumination wavelength of filtering, obtains the effective royal purple light of Growth of Dendrobium candidum.
UVB in above indication UVB irradiation, i.e. ultraviolet radiation B are the wave bands dividing out by ultraviolet wavelength according to the difference of biological effect, its wavelength 275 ~ 320nm.
The invention has the beneficial effects as follows:
(1) cultivation method of the present invention improves the content of active component polysaccharides class and phenols component in dendrobium candidum effectively, improves the quality of dendrobium candidum, has very important medical value and economic worth.
(2) culture technique of the present invention significantly can reduce the generation of dendrobium candidum damage by disease and insect, is conducive to production management and the output increased of dendrobium candidum.UVB irradiation can effectively suppress or pathogenic microbe killing, the rotten leaf that can effectively prevent from generally occurring because of the infection of pathogenic microorganism in Growth of Dendrobium candidum process, the phenomenon such as rotten.
(3) culture technique of the present invention by installing UVB fluorescent tube to reach regulation and control Growth of Dendrobium candidum to the requirement of light quality in booth, and method is simply easy to operation, is suitable for the requirement of the large production of dendrobium candidum scale.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but be not limited thereto.
embodiment 1
Installing in the booth of UVB irradiation fluorescent tube and automatic time switch with bluish purple covered rearing with plastic film, plantation dendrobium candidum seedling, treat that it grows Xin Gen and sprouting, show after transplant survival, come into effect UVB irradiation, and till being continued until when gathering, exposure intensity is 0.35 μ Wcm
-2, the irradiation time of every day is 5 o'clock to 6 o'clock totally 1 hour afternoon, and during UVB irradiation, spray in seedbed, keeps the humidity of dendrobium officinale culture medium more than 70%, and temperature is between 25 ~ 35 DEG C, and other production management is with the production management routinized.After it is gathered, the content of wherein Dendrobium officinale polysaccharide class and phenols component is measured by the method for Chinese Pharmacopoeia, and with cultivation (the plastic house White plastic film that routinizes, do not implement UVB irradiation) dendrobium candidum compare, after recording UVB irradiation, the content of polysaccharide and phenols is respectively 42.8%, 2.53%, and in contrast dendrobium candidum medicinal material, the content of polysaccharide and phenols is respectively 28.6% and 2.23%.After UVB irradiation, in dendrobium candidum, the content of polysaccharide and phenols component improves 49.7% and 13.5% respectively.
embodiment 2
Installing in the booth of UVB irradiation fluorescent tube and automatic time switch with bluish purple covered rearing with plastic film, moved in this booth by potted plant dendrobium candidum 30 basin of having planted a year and grow, implement UVB irradiation 1 month, UVB irradiation intensity is 6.5 μ Wcm
-2, the irradiation time of every day be 8 a.m. to 11 totally 3 hours, during UVB irradiation, spray in seedbed, keeps the humidity of dendrobium officinale culture medium more than 70%, and temperature is between 25 ~ 35 DEG C, and other production management is with the production management routinized.UVB irradiation is after 1 month, again retract in conventional booth and grow, after it is gathered, the content of wherein Dendrobium officinale polysaccharide class and phenols component is measured by the method for Chinese Pharmacopoeia, and compare with the dendrobium candidum of the cultivation that routinizes, after recording UVB irradiation, the content of polysaccharide and phenols is respectively 35.2%, 2.45%, and in contrast dendrobium candidum medicinal material, the content of polysaccharide and phenols is respectively 28.6% and 2.23%.After UVB irradiation, in dendrobium candidum, the content of polysaccharide and phenols component improves 23.1% and 9.8% respectively.
embodiment 3
At first trimester of gathering, the potted plant dendrobium candidum that routinized by 30 basins moves into bluish purple covered rearing with plastic film and installs in the booth of UVB irradiation fluorescent tube and automatic time switch, comes into effect UVB irradiation, and till being continued until when gathering, exposure intensity is 30 μ Wcm
-2, the irradiation time of every day is 3 o'clock to 9 o'clock totally 6 hours afternoon, and during UVB irradiation, spray in seedbed, keeps the humidity of dendrobium officinale culture medium more than 70%, and temperature is between 25 ~ 35 DEG C, and other production management is with the production management routinized.After it is gathered, the content of wherein Dendrobium officinale polysaccharide class and phenols component is measured by the method for Chinese Pharmacopoeia, and compare with the dendrobium candidum of the cultivation that routinizes, after recording UVB irradiation, the content of polysaccharide and phenols is respectively 44.5%, 3.68%, and in contrast dendrobium candidum medicinal material, the content of polysaccharide and phenols is respectively 28.6% and 2.23%.After UVB irradiation, in dendrobium candidum, the content of polysaccharide and phenols component improves 55.6% and 65.0% respectively.
contrast:
In the officinal dendrobium stem plantation booth routinizing cultivation, namely cover with White plastic film and do not install in UVB irradiation fluorescent tube booth, plantation and the dendrobium candidum seedling of the same provenance of UVB radiation treatment, cultivate by the productive function that routinizes of dendrobium candidum greenhouse cultivation.After it is gathered, measure the content of wherein Dendrobium officinale polysaccharide class and phenols component by the method for Chinese Pharmacopoeia, the content recording polysaccharide and phenols in UVB dendrobium candidum medicinal material is respectively 28.6% and 2.23%.Compared with comparative example, UVB irradiation significantly improves the content of polysaccharose and phenols component in dendrobium candidum.
Above embodiment shows, culture technique of the present invention improves the content of active component polysaccharides class and phenols component in dendrobium candidum effectively, improves the quality of dendrobium candidum, has very important medical value and economic worth.
Above embodiment is only introduces preferred case of the present invention, to those skilled in the art, not deviating from any apparent changes and improvements of carrying out in the scope of spirit of the present invention, all should be regarded as a part of the present invention.
Claims (5)
1. one kind is improved the cultivation method of polysaccharose and phenols component in dendrobium candidum, comprise and in the course of cultivation UVB radiation is manually increased to dendrobium candidum, the content of polysaccharose and phenols component in dendrobium candidum stem can be made to improve 23.1 ~ 55.6% and 9.8 ~ 65.0% respectively;
It is characterized in that, UVB irradiation intensity is 0.35 ~ 30 μ W.cm
-2.
2. cultivation method according to claim 1, is characterized in that, described UVB irradiation refers to that any stage in officinal dendrobium stem plantation process implements UVB and irradiates more than 1 month.
3. cultivation method according to claim 1, is characterized in that, described UVB irradiation refers to and to carry out in 3 months before dendrobium candidum is gathered, and every day is irradiation more than 1 hour at least.
4. the cultivation method according to any one of claims 1 to 3, is characterized in that, in UVB irradiation process, takes seedbed to spray, and keep the humidity of dendrobium officinale culture medium more than 70%, temperature is between 25 ~ 35 DEG C.
5. the cultivation method according to any one of claims 1 to 3, is characterized in that, described officinal dendrobium stem plantation is in booth, and booth is with bluish purple plastic film covering.
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| CN109997622A (en) * | 2019-04-17 | 2019-07-12 | 广州市八斗农业科技有限公司 | The method for improving dendrobium candidum active constituent content |
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| CN104026016A (en) * | 2014-06-20 | 2014-09-10 | 山东理工大学 | Method for producing dendrobium polysaccharides by inducing dendrobium candidum by jasmonic acid methyl ester |
| CN104285632A (en) * | 2014-09-10 | 2015-01-21 | 韦俊 | Cultivation method for improving polysaccharide content of Chinese yam |
| CN105724163A (en) * | 2016-02-24 | 2016-07-06 | 吕强 | Peach tree fruiting period management method |
| CN107771554B (en) * | 2017-06-27 | 2020-12-08 | 华南农业大学 | Method for improving seedling vegetable quality by using blue and violet LED intermittent supplementary lighting |
| KR20210030943A (en) * | 2018-08-09 | 2021-03-18 | 서울바이오시스 주식회사 | Plant cultivation apparatus and cultivation method using the same |
| CN115777375B (en) * | 2022-12-21 | 2023-10-24 | 广东省农业科学院环境园艺研究所 | UV-A radiation method for improving active substance content of dendrobium nobile |
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