CN103704035B - A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component - Google Patents

A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component Download PDF

Info

Publication number
CN103704035B
CN103704035B CN201310734851.4A CN201310734851A CN103704035B CN 103704035 B CN103704035 B CN 103704035B CN 201310734851 A CN201310734851 A CN 201310734851A CN 103704035 B CN103704035 B CN 103704035B
Authority
CN
China
Prior art keywords
dendrobium candidum
dendrobium
cultivation method
uvb
phenols
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201310734851.4A
Other languages
Chinese (zh)
Other versions
CN103704035A (en
Inventor
曾令杰
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Guangdong Pharmaceutical University
Original Assignee
Guangdong Pharmaceutical University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Pharmaceutical University filed Critical Guangdong Pharmaceutical University
Priority to CN201310734851.4A priority Critical patent/CN103704035B/en
Publication of CN103704035A publication Critical patent/CN103704035A/en
Application granted granted Critical
Publication of CN103704035B publication Critical patent/CN103704035B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

Landscapes

  • Cultivation Of Plants (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component, comprise and in the course of cultivation UVB radiation is manually increased to dendrobium candidum, the content of polysaccharose and phenols component in dendrobium candidum stem can be made to improve 23.1 ~ 55.6% and 9.8 ~ 65.0% respectively.Cultivation method of the present invention improves the content of active component polysaccharides class and phenols component in dendrobium candidum effectively, improves the quality of dendrobium candidum, has very important medical value and economic worth.And this method is simply easy to operation, is suitable for the requirement of the large production of dendrobium candidum scale.

Description

A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component
Technical field
The invention belongs to field of plant cultivation, be specifically related to a kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component.
Background technology
Dendrobium candidum dendrobium officinalekimura et Migo is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, because its eustipes part is pitchy, has another name called ribbed hedyotis herb.Its fresh or dry stem uses as medicinal material, and be rare crude drugs, medicinal history is long, is classified as top grade in Shennong's Herbal, has nourishing Yin and clearing heat, and promote the production of body fluid beneficial stomach, and the effect such as moisten the lung and relieve the cough.The dendrobium candidum stem of noble dendrobium requires harsher to growing environment, needs special ecological environmental condition, and dendrobium candidum stem of noble dendrobium wild resource is few, within 1987, is classified as one of animals and plants medicinal material of dying out in imminent danger by country.
Main active in dendrobium candidum is polysaccharose wherein and phenols component.Polysaccharose composition is the active component (Li Manfei etc. in dendrobium candidum with immunological enhancement and antitumor action, Chinese herbal medicine, 1990,21 (10): 10), and content higher (yellow civil rights etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 1997,22(2): 74), the content of " Chinese Pharmacopoeia " 2010 editions regulation Dendrobium officinale polysaccharides must not be less than 25%.Phenols component is a compound class phenyl ring with hydroxyl, phenols component in dendrobium candidum mainly stilbene compound, (Malaysian is auspicious to comprise two benzyl phenols and phenanthrol compounds EN and chrysotoxene, phenanthrenequione parent nucleus HERBA DENDROBII phenanthrenequione, Bibenzyl compound and dendrophnol etc., China Medicine University's journal, 1994,25 (3): 188 ~ 189; Li Yan etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2010,35 (13): 1715-1719), its pharmacologically active is mainly antitumor.In addition, alkaloids composition (0.02% ~ 0.04%) containing trace in dendrobium candidum, but the content of alkaloids composition is only 1/20 of HERBA DENDROBII in dendrobium candidum, alkaloid is not the main component (Chen Xiaomei etc. of dendrobium candidum, Chinese Academy of Medical Sciences's journal, 2006,28 (4): 524 ~ 529; Zhu Yan etc., CHINA JOURNAL OF CHINESE MATERIA MEDICA, 2010,35 (18): 2388-2390).Thus, the quality of how many direct reflection dendrobium candidum qualities of polysaccharose composition and phenols component in dendrobium candidum, the content being improved polysaccharose and phenols component in dendrobium candidum by cultivation management method is had great importance for the dendrobium candidum of production high-quality.
Summary of the invention
The object of the present invention is to provide a kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component.
The technical solution used in the present invention is:
Improve a cultivation method for polysaccharose and phenols component in dendrobium candidum, comprise and in the course of cultivation UVB radiation is manually increased to dendrobium candidum, the content of polysaccharose and phenols component in dendrobium candidum stem can be made to improve 23.1 ~ 55.6% and 9.8 ~ 65.0% respectively.
Described UVB irradiation refers to that any stage in officinal dendrobium stem plantation process implements UVB and irradiates more than 1 month.
As preferably, described UVB irradiation refers to and to carry out in 3 months before dendrobium candidum is gathered, and every day is irradiation more than 1 hour at least.
UVB irradiation intensity is 0.35 ~ 30 μ W.cm -2.
In UVB irradiation process, take seedbed to spray, keep the humidity of dendrobium officinale culture medium more than 70%, temperature, between 25 ~ 35 DEG C, to eliminate the adverse effects such as fuel factor that UVB irradiation brings, improves the resistance of dendrobium candidum.
Described officinal dendrobium stem plantation is in booth, and booth, with bluish purple plastic film covering, in order to other illumination wavelength of filtering, obtains the effective royal purple light of Growth of Dendrobium candidum.
UVB in above indication UVB irradiation, i.e. ultraviolet radiation B are the wave bands dividing out by ultraviolet wavelength according to the difference of biological effect, its wavelength 275 ~ 320nm.
The invention has the beneficial effects as follows:
(1) cultivation method of the present invention improves the content of active component polysaccharides class and phenols component in dendrobium candidum effectively, improves the quality of dendrobium candidum, has very important medical value and economic worth.
(2) culture technique of the present invention significantly can reduce the generation of dendrobium candidum damage by disease and insect, is conducive to production management and the output increased of dendrobium candidum.UVB irradiation can effectively suppress or pathogenic microbe killing, the rotten leaf that can effectively prevent from generally occurring because of the infection of pathogenic microorganism in Growth of Dendrobium candidum process, the phenomenon such as rotten.
(3) culture technique of the present invention by installing UVB fluorescent tube to reach regulation and control Growth of Dendrobium candidum to the requirement of light quality in booth, and method is simply easy to operation, is suitable for the requirement of the large production of dendrobium candidum scale.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but be not limited thereto.
embodiment 1
Installing in the booth of UVB irradiation fluorescent tube and automatic time switch with bluish purple covered rearing with plastic film, plantation dendrobium candidum seedling, treat that it grows Xin Gen and sprouting, show after transplant survival, come into effect UVB irradiation, and till being continued until when gathering, exposure intensity is 0.35 μ Wcm -2, the irradiation time of every day is 5 o'clock to 6 o'clock totally 1 hour afternoon, and during UVB irradiation, spray in seedbed, keeps the humidity of dendrobium officinale culture medium more than 70%, and temperature is between 25 ~ 35 DEG C, and other production management is with the production management routinized.After it is gathered, the content of wherein Dendrobium officinale polysaccharide class and phenols component is measured by the method for Chinese Pharmacopoeia, and with cultivation (the plastic house White plastic film that routinizes, do not implement UVB irradiation) dendrobium candidum compare, after recording UVB irradiation, the content of polysaccharide and phenols is respectively 42.8%, 2.53%, and in contrast dendrobium candidum medicinal material, the content of polysaccharide and phenols is respectively 28.6% and 2.23%.After UVB irradiation, in dendrobium candidum, the content of polysaccharide and phenols component improves 49.7% and 13.5% respectively.
embodiment 2
Installing in the booth of UVB irradiation fluorescent tube and automatic time switch with bluish purple covered rearing with plastic film, moved in this booth by potted plant dendrobium candidum 30 basin of having planted a year and grow, implement UVB irradiation 1 month, UVB irradiation intensity is 6.5 μ Wcm -2, the irradiation time of every day be 8 a.m. to 11 totally 3 hours, during UVB irradiation, spray in seedbed, keeps the humidity of dendrobium officinale culture medium more than 70%, and temperature is between 25 ~ 35 DEG C, and other production management is with the production management routinized.UVB irradiation is after 1 month, again retract in conventional booth and grow, after it is gathered, the content of wherein Dendrobium officinale polysaccharide class and phenols component is measured by the method for Chinese Pharmacopoeia, and compare with the dendrobium candidum of the cultivation that routinizes, after recording UVB irradiation, the content of polysaccharide and phenols is respectively 35.2%, 2.45%, and in contrast dendrobium candidum medicinal material, the content of polysaccharide and phenols is respectively 28.6% and 2.23%.After UVB irradiation, in dendrobium candidum, the content of polysaccharide and phenols component improves 23.1% and 9.8% respectively.
embodiment 3
At first trimester of gathering, the potted plant dendrobium candidum that routinized by 30 basins moves into bluish purple covered rearing with plastic film and installs in the booth of UVB irradiation fluorescent tube and automatic time switch, comes into effect UVB irradiation, and till being continued until when gathering, exposure intensity is 30 μ Wcm -2, the irradiation time of every day is 3 o'clock to 9 o'clock totally 6 hours afternoon, and during UVB irradiation, spray in seedbed, keeps the humidity of dendrobium officinale culture medium more than 70%, and temperature is between 25 ~ 35 DEG C, and other production management is with the production management routinized.After it is gathered, the content of wherein Dendrobium officinale polysaccharide class and phenols component is measured by the method for Chinese Pharmacopoeia, and compare with the dendrobium candidum of the cultivation that routinizes, after recording UVB irradiation, the content of polysaccharide and phenols is respectively 44.5%, 3.68%, and in contrast dendrobium candidum medicinal material, the content of polysaccharide and phenols is respectively 28.6% and 2.23%.After UVB irradiation, in dendrobium candidum, the content of polysaccharide and phenols component improves 55.6% and 65.0% respectively.
contrast:
In the officinal dendrobium stem plantation booth routinizing cultivation, namely cover with White plastic film and do not install in UVB irradiation fluorescent tube booth, plantation and the dendrobium candidum seedling of the same provenance of UVB radiation treatment, cultivate by the productive function that routinizes of dendrobium candidum greenhouse cultivation.After it is gathered, measure the content of wherein Dendrobium officinale polysaccharide class and phenols component by the method for Chinese Pharmacopoeia, the content recording polysaccharide and phenols in UVB dendrobium candidum medicinal material is respectively 28.6% and 2.23%.Compared with comparative example, UVB irradiation significantly improves the content of polysaccharose and phenols component in dendrobium candidum.
Above embodiment shows, culture technique of the present invention improves the content of active component polysaccharides class and phenols component in dendrobium candidum effectively, improves the quality of dendrobium candidum, has very important medical value and economic worth.
Above embodiment is only introduces preferred case of the present invention, to those skilled in the art, not deviating from any apparent changes and improvements of carrying out in the scope of spirit of the present invention, all should be regarded as a part of the present invention.

Claims (5)

1. one kind is improved the cultivation method of polysaccharose and phenols component in dendrobium candidum, comprise and in the course of cultivation UVB radiation is manually increased to dendrobium candidum, the content of polysaccharose and phenols component in dendrobium candidum stem can be made to improve 23.1 ~ 55.6% and 9.8 ~ 65.0% respectively;
It is characterized in that, UVB irradiation intensity is 0.35 ~ 30 μ W.cm -2.
2. cultivation method according to claim 1, is characterized in that, described UVB irradiation refers to that any stage in officinal dendrobium stem plantation process implements UVB and irradiates more than 1 month.
3. cultivation method according to claim 1, is characterized in that, described UVB irradiation refers to and to carry out in 3 months before dendrobium candidum is gathered, and every day is irradiation more than 1 hour at least.
4. the cultivation method according to any one of claims 1 to 3, is characterized in that, in UVB irradiation process, takes seedbed to spray, and keep the humidity of dendrobium officinale culture medium more than 70%, temperature is between 25 ~ 35 DEG C.
5. the cultivation method according to any one of claims 1 to 3, is characterized in that, described officinal dendrobium stem plantation is in booth, and booth is with bluish purple plastic film covering.
CN201310734851.4A 2013-12-27 2013-12-27 A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component Expired - Fee Related CN103704035B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310734851.4A CN103704035B (en) 2013-12-27 2013-12-27 A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310734851.4A CN103704035B (en) 2013-12-27 2013-12-27 A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component

Publications (2)

Publication Number Publication Date
CN103704035A CN103704035A (en) 2014-04-09
CN103704035B true CN103704035B (en) 2015-09-02

Family

ID=50397713

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310734851.4A Expired - Fee Related CN103704035B (en) 2013-12-27 2013-12-27 A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component

Country Status (1)

Country Link
CN (1) CN103704035B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109997622A (en) * 2019-04-17 2019-07-12 广州市八斗农业科技有限公司 The method for improving dendrobium candidum active constituent content

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104026016A (en) * 2014-06-20 2014-09-10 山东理工大学 Method for producing dendrobium polysaccharides by inducing dendrobium candidum by jasmonic acid methyl ester
CN104285632A (en) * 2014-09-10 2015-01-21 韦俊 Cultivation method for improving polysaccharide content of Chinese yam
CN105724163A (en) * 2016-02-24 2016-07-06 吕强 Peach tree fruiting period management method
CN107771554B (en) * 2017-06-27 2020-12-08 华南农业大学 Method for improving seedling vegetable quality by using blue and violet LED intermittent supplementary lighting
KR20210030943A (en) * 2018-08-09 2021-03-18 서울바이오시스 주식회사 Plant cultivation apparatus and cultivation method using the same
CN115777375B (en) * 2022-12-21 2023-10-24 广东省农业科学院环境园艺研究所 UV-A radiation method for improving active substance content of dendrobium nobile

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101904497A (en) * 2010-07-16 2010-12-08 湖北工业大学 Method for improving vitamin D content in lucid ganoderma calcium fortifier by ultraviolet irradiation
CN101953331A (en) * 2009-07-17 2011-01-26 李宣和 Crops insect removing apparatus equipped with multi-spectrum filter part therein

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4887709B2 (en) * 2005-09-27 2012-02-29 パナソニック電工株式会社 Method and apparatus for increasing yield of plant polyphenols
JP4280835B2 (en) * 2006-10-03 2009-06-17 国立大学法人山口大学 Method for enhancing nutritional components of plants
KR20110055811A (en) * 2009-11-20 2011-05-26 한국식품연구원 Method for increasing polyphenol quantity in ginseng and apparatus therefor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101953331A (en) * 2009-07-17 2011-01-26 李宣和 Crops insect removing apparatus equipped with multi-spectrum filter part therein
CN101904497A (en) * 2010-07-16 2010-12-08 湖北工业大学 Method for improving vitamin D content in lucid ganoderma calcium fortifier by ultraviolet irradiation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
UV-B辐射对豌豆伸长生长和细胞壁多糖组分的影响;曲颖等;《辐射研究与辐射工艺学报》;20121031;第30卷(第5期);第303-308页 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109997622A (en) * 2019-04-17 2019-07-12 广州市八斗农业科技有限公司 The method for improving dendrobium candidum active constituent content

Also Published As

Publication number Publication date
CN103704035A (en) 2014-04-09

Similar Documents

Publication Publication Date Title
CN103704035B (en) A kind of cultivation method improving Dendrobium officinale polysaccharide class and phenols component
Zhang et al. Changes in bioactive compounds and antioxidant activities in pomegranate leaves
CN103004445B (en) Method for directly seedling dendrobe seedlings by using plastic greenhouse
CN104067838B (en) A kind of implantation methods of radix polygonati officinalis
CN105248140A (en) Seedling culturing method for polygonatum cyrtonema seeds
CN105284393A (en) Method for seedling sealwort seeds
CN105493861B (en) A kind of method of bletilla protocorm live streaming
CN103999750B (en) A kind of propagation method of botrychiam ternatum spore
CN104429865A (en) Sexual propagation method of bletilla striata
CN101461327A (en) Rapid propagation technique of Paris polyphylla var. yunnanensis, seedling exercising and planting methods
CN105532471A (en) Novel high-yield, high-quality and large-scale polygonatum cyrtonema cultivation method
CN106083383A (en) Seedling bed of Herba Dendrobii and preparation method thereof
CN106818359A (en) A kind of fast seedling-cultivating method of tea tree
CN104871753B (en) A kind of red Chinese toon method for culturing seedlings
CN101990815A (en) Domestic planting method of pingpien ginseng rhizome
CN107711115A (en) A kind of method of porcupine quill cuttage and seedling culture
CN108157098B (en) Seedling hardening and transplanting method for tissue culture rooted seedlings of bletilla striata
CN103907460A (en) Cultivation method for one-time seedling input, repeated planting and multiple harvests of Paris polyhoylla var chinens
CN106717275A (en) A kind of germination accelerating method of levisticum seed
CN105981554A (en) Greenhouse original ecology planting method for Dendrobium officinale
CN109644616A (en) A kind of fast breeding method of limacia sagittata seedlings
CN104798567A (en) High-yield planting method of salix integra
CN107155622A (en) A kind of implantation methods of young Chinese toon seedling
CN104738151B (en) A kind of method for improving rosmarinic acid contents in selfheal leaf
CN108370677A (en) The implantation methods of huangteng

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CP03 Change of name, title or address
CP03 Change of name, title or address

Address after: 510240 40 Haizhuqu District, Guangdong, Guangzhou.

Patentee after: Guangdong Pharmaceutical University

Address before: 510006 No. 280 East Ring Road, Guangzhou City University, Guangdong

Patentee before: Guangdong Pharmaceutical University

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150902

Termination date: 20181227