CN103004445A - Method for directly seedling dendrobe seedlings by using plastic greenhouse - Google Patents

Method for directly seedling dendrobe seedlings by using plastic greenhouse Download PDF

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CN103004445A
CN103004445A CN2013100043977A CN201310004397A CN103004445A CN 103004445 A CN103004445 A CN 103004445A CN 2013100043977 A CN2013100043977 A CN 2013100043977A CN 201310004397 A CN201310004397 A CN 201310004397A CN 103004445 A CN103004445 A CN 103004445A
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seedbed
stem
matrix
noble dendrobium
seedling
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CN2013100043977A
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CN103004445B (en
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戴亚峰
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戴亚峰
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/25Greenhouse technology, e.g. cooling systems therefor

Abstract

A method for directly seedling dendrobe seedlings by using a plastic greenhouse comprises the following steps: constructing a greenhouse; making a seedling bed; preparing a base material and performing sterilization, which can be realized by mixing pine barks and sheep manure, filtering the pine barks and the sheep manure through a screen after completely fermenting the pine barks and the sheep manure, soaking the pine barks and the sheep manure by using carbendazim, spreading the pine barks and the sheep manure on the seedling bed, placing the pine barks and the sheep manure on the seedling bed for 5-10 days, and controlling the water content; inoculating the symbiotic bacteria, which can be realized by taking a two years old base material of the dendrobe and uniformly scattering the two years old substrate on the seedling bed on which a new substrate is laid; pre-treating seeds, which can be realized by completely moisturizing the seeds by using sterile water containing naphthyl acetic acid; sterilizing the seeds in the greenhouse, which can be realized by fumigating every cubic meter of seeds by using 4 g mushroom protector, fumigating the seeds for one time before sowing, and fumigating the seeds every half a month in the future; scattering, which can be realized by after the seeds are scattered and before the seedlings are transplanted, controlling temperature in the greenhouse, water content of the seedling bed and air humidity, treating the seeds by weak lights within the first month of the scattering, and gradually enhancing illumination after budding; managing water, fertilizers and diseases of the seedlings; and transplanting the seedlings. The method for directly seedling the dendrobe seedlings by using the plastic greenhouse, provided by the invention has the advantages that as the dendrobe are transplanted after directly becoming to the seedlings, the seedling breeding cost is effectively reduced, the seedling rate of the seeds is more than 90 percent, and the transplanting survival rate of the seedlings is 100 percent.

Description

The method of the live stem of noble dendrobium seedling of plastic tunnel
Technical field
The present invention relates to the growing and cultivation field of the stem of noble dendrobium, be specifically related to the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel.
Background technology
Dendrobium the orchid family herbaceos perennial, the common kind of the stem of noble dendrobium has: HERBA DENDROBII, dendrobium candidum, purple dendrobium, Dendrobidium huoshanness etc., the part stem of noble dendrobium can be used as medicine, and human body is driven the solution abnormal heat, the curative effects such as beneficial smart reinforcing yin essence.
Dendrobidium huoshanness is commonly called as " rice dry measure used in former times ", is the superfine product in the stem of noble dendrobium, mainly is distributed in the Huoshan in the west and south, Anhui.Because of the compositions such as its contained alkaloid, thick polysaccharide, amino acid, trace element have strong kidney benefit essence, thick stomach, inhibition tumor cell, hypoglycemic, strengthen the effects such as immunity, alleviating physical fatigue, be regarded as first of the China nine large " celestial careless " always.For a long time, because of the growth conditions of Dendrobidium huoshanness and harshness thereof and people to its excessive harvesting, cause these species endangered.
Numerous companies and scientific research institutions have realized conservation and rational exploitation and utilization to the stem of noble dendrobium by modern biotechnology.Its general process is: tissue culture, and hardening, domestication is transplanted, and gathers deep processing.
Relating to medium in this process of tissue culture makes, aseptic access explant, protocorm is aseptic to be turned minute, and Bud Differentiation is aseptic to be turned minute, seedling is aseptic to be turned minute, middle seedling is aseptic to be turned minute, and seedlings are aseptic to be turned minute, the steps such as culturing room's management, relate to thus minute the Miaos person's recruitment and management, the construction of medium atelier, the construction in sterile working workshop, the construction of culturing room; And relate to related personnel's recruitment and management, the construction of intelligent greenhouse and domestication booth in the hardening, domestication process; The survival rate of transplanting simultaneously is low, is about 70%-75% even lower.In sum, the prior art aspect requires enterprise all sizable input will be arranged on human and material resources, financial resources, causes thus that entreprise cost is large, burden is heavy, simultaneously one of transplanting survival rate primary factor that has become the development of restriction stem of noble dendrobium industrial scale on the low side.
Summary of the invention
Technical problem to be solved by this invention provide a kind of effectively reduce entreprise cost, seed planting percent up to more than 90% and transplanting survival rate up to the method for the live stem of noble dendrobium seedling of 100% plastic tunnel.
The present invention solves the problems of the technologies described above by the following technical solutions: the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel may further comprise the steps:
1, build booth: the specification of booth does not have special requirement, and the people can enter to operate namely can.Plastic covering film and sunshade net above the booth are used for rainwater-proof and stop sunray, make the highest 20000 luxs that are no more than of illumination.Optimize, for easy to operate, described greenhouse is high 2.5~4 meters, and is wide 5~7 meters.Also be as the scheme of optimizing, for rain proofness and stop that the sunshine effect is better, cover the sunshade net of 6~9 thick plastic foils and sunshade rate 70 ﹪~90% above the booth.
2, make the seedbed: the seedbed is the built on stilts plastic deeping in bottom, is beneficial to the bottom and ventilates, and the specification in seedbed does not have special requirement, can put booth into and handled easily gets final product.Optimize, plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel.Optimize, for easy to operate, the seedbed is wide 1~2 meter, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed (3~5): 1 mixes, and rotten thoroughly rear 3~5 purpose screen clothes of crossing of fermentation soaked 3~5 minutes with 600 times carbendazim, then pave on the seedbed, 8~12 centimetres of thickness were placed 5~10 days, and control seedbed moisture content gets final product at 60 ﹪~65 ﹪.
4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 3~5 purpose screen clothes, by every square metre of consumption that spreads 1~1.5 kilogram of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.All with the symbiosis fungi, and stem of noble dendrobium seed namely not have the nutriment of its growth of confession because there is not endosperm to the stem of noble dendrobium in outdoor growth, and the root symbiosis mycosymbiosis in the time of must growing with the stem of noble dendrobium relies on it that nutrient is provided, and could sprout.
5, seed preliminary treatment: with the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 4~6 minutes, then cut open, seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 4~6 hours, allow kind of a skin be filled and contain the hormone sterile water.
6, sterilize in the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
7, broadcast sowing: begin to sow the spring that turns after warming up at outside air temperature, also be outdoor temperature 17~30 degree, get 0.8~1.2 kilogram of matrix in the seedbed first, mix 8~12 seeds that soaked hormone, then be sprinkling upon on the seedbed after mixing thoroughly, area is 8~12 square metres.
8, temperature, light, humidity control: to transplanting, temperature of shed is controlled between 18 ℃~30 ℃, and is the most suitable with 28 ℃ after broadcasting sowing; The seedbed moisture control is at 60%~65 ﹪, and air humidity maintains 60 ﹪~80 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: keep seedbed moisture content 60%~65 ﹪ with sprinkling equipment, air humidity 60 ﹪~80 ﹪, wait the stem of noble dendrobium to extract two leaves out after, spray weekly a flower foliage fertilizer of 1 to 2 time 1 ‰; Daily disease by sprayed in per ten days one time 600 times carbendazim or 750 times thiophanate methyl get final product.
10, transplant: the spring of Second Year, the stem of noble dendrobium seedling of complete root, stem, leaf will be possessed, rise together with matrix one digging, optimize, seeding row spacing by 15 centimetres of 10 cm x is transplanted, can certainly transplant according to other suitable seeding row spacings, this all is prior art, and follow-up management gets final product by existing planting type.
The invention has the advantages that: directly stem of noble dendrobium high quality seed is broadcast on treated good seedbed, by the cultivation management of science, directly transplanted again after the seedling.Can effectively reduce entreprise cost, and the seed planting percent is up to more than 90%, and transplanting survival rate is up to 100%.Can live Dendrobidium huoshanness, the various stem of noble dendrobium kind seedlings, particularly Dendrobidium huoshanness such as HERBA DENDROBII, dendrobium candidum, purple dendrobium, have huge commercial promise.
Embodiment
Embodiment one
The method of the live Dendrobidium huoshanness seedling of a kind of plastic tunnel may further comprise the steps:
1, build booth: greenhouse is high 3 meters, and is wide 6 meters, covers the sunshade net of 8 thick plastic foils and sunshade rate 90 ﹪ above the booth.
2, make the seedbed: the seedbed is the built on stilts plastic deeping in bottom, optimization, plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 1.5 meters, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed 4:1 mix, fermentation is rotten crosses 4 purpose screen clothes after thoroughly, soaks 4 minutes with 600 times carbendazim, then paves on the seedbed, and 10 centimetres of thickness were placed 7 days, controlled the seedbed moisture content at 60 ﹪.
4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 4 purpose screen clothes, by every square metre of consumption that spreads 1 kilogram of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.
5, seed preliminary treatment: with the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 5 minutes, then cut open, seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 5 hours, allow kind of a skin be filled and contain the hormone sterile water.
6, sterilize in the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
7, broadcast sowing: begin sowing the spring that turns after warming up at outside air temperature, also is outdoor temperature 17~25 degree, gets 1 kilogram of matrix in the seedbed first, mixes 10 seeds that soaked hormone, is sprinkling upon on the seedbed after then mixing thoroughly, and area is 10 square metres.
8, temperature, light, humidity control: to transplanting, temperature of shed is controlled at 28 ℃ after broadcasting sowing, and the seedbed moisture control is at 60 ﹪, and air humidity maintains 70 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: keep seedbed moisture content 60 ﹪ with sprinkling equipment, air humidity 70 ﹪, wait the stem of noble dendrobium to extract two leaves out after, spray weekly a flower foliage fertilizer of 1 time 1 ‰; Daily disease is by spraying 600 times carbendazim in per ten days one time.
10, transplant: with possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging the spring of Second Year, transplant by the seeding row spacing of 15 centimetres of 10 cm x.Follow-up management gets final product by existing planting type.
Embodiment two
The method of the live HERBA DENDROBII seedling of a kind of plastic tunnel may further comprise the steps:
1, build booth: described greenhouse is high 2.5 meters, and is wide 5 meters, covers the sunshade net of 6 thick plastic foils and sunshade rate 70 ﹪ above the booth.
2, make the seedbed: the seedbed is the built on stilts plastic deeping in bottom, and plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 1 meter, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed 3:1 mix, fermentation is rotten crosses 3 purpose screen clothes after thoroughly, soaks 5 minutes with 600 times carbendazim, then paves on the seedbed, and 8 centimetres of thickness were placed 5 days, controlled the seedbed moisture content and got final product at 65 ﹪.
4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 3 purpose screen clothes, by every square metre of consumption that spreads 1.2 kilograms of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.All with the symbiosis fungi, and stem of noble dendrobium seed namely not have the nutriment of its growth of confession because there is not endosperm to the stem of noble dendrobium in outdoor growth, and the root symbiosis mycosymbiosis in the time of must growing with the stem of noble dendrobium relies on it that nutrient is provided, and could sprout.
5, seed preliminary treatment: with the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 4 minutes, then cut open, seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 4 hours, allow kind of a skin be filled and contain the hormone sterile water.
6, sterilize in the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
7, broadcast sowing: begin sowing the spring that turns after warming up at outside air temperature, also is outdoor temperature 17~25 degree, gets 0.8 kilogram of matrix in the seedbed first, mixes 8 seeds that soaked hormone, is sprinkling upon on the seedbed after then mixing thoroughly, and area is 8 square metres.
8, temperature, light, humidity control: to transplanting, temperature of shed is controlled at 18 ℃ after broadcasting sowing, and the seedbed moisture control is 65%, and air humidity maintains 70 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: keep seedbed moisture content 65% with sprinkling equipment, air humidity 70 ﹪, wait the stem of noble dendrobium to extract two leaves out after, spray weekly a flower foliage fertilizer of 1 time 1 ‰; Daily disease gets final product by spraying a thiophanate methyl of 750 times in per ten days.
10, transplant: with possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging the spring of Second Year, transplant by the seeding row spacing of 12 centimetres of 8 cm x.Follow-up management gets final product by existing planting type.
Embodiment three
The method of the live dendrobium candidum seedling of a kind of plastic tunnel may further comprise the steps:
1, build booth: described greenhouse is high 4 meters, and is wide 7 meters, covers the sunshade net of 9 thick plastic foils and sunshade rate 90 ﹪ above the booth.
2, make the seedbed: the seedbed is the built on stilts plastic deeping in bottom, and plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 2 meters, and length is as the criterion with canopy length.
3, matrix and sterilization: pine bark and sheep excrement are pressed 5:1 mix, fermentation is rotten crosses 5 purpose screen clothes after thoroughly, soaks 3 minutes with 550 times carbendazim, then paves on the seedbed, and 12 centimetres of thickness were placed 10 days, controlled the seedbed moisture content and got final product at 62 ﹪.
4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 5 purpose screen clothes, by every square metre of consumption that spreads 1.5 kilograms of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly.All with the symbiosis fungi, and stem of noble dendrobium seed namely not have the nutriment of its growth of confession because there is not endosperm to the stem of noble dendrobium in outdoor growth, and the root symbiosis mycosymbiosis in the time of must growing with the stem of noble dendrobium relies on it that nutrient is provided, and could sprout.
5, seed preliminary treatment: with the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 6 minutes, then cut open, seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate (NAA) and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine (6-BA) and infiltrate 6 hours, allow kind of a skin be filled and contain the hormone sterile water.
6, sterilize in the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
7, broadcast sowing: begin sowing the spring that turns after warming up at outside air temperature, also is outdoor temperature 17~25 degree, gets 1.2 kilograms of matrix in the seedbed first, mixes 12 seeds that soaked hormone, is sprinkling upon on the seedbed after then mixing thoroughly, and area is 12 square metres.
8, temperature, light, humidity control: to transplanting, temperature of shed is controlled at 25 ℃ after broadcasting sowing, and the seedbed moisture control is 62%, and air humidity maintains 80 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than.
9, water, fertilizer, plant disease management: keep seedbed moisture content 62% with sprinkling equipment, air humidity 80 ﹪, wait the stem of noble dendrobium to extract two leaves out after, spray weekly a flower foliage fertilizer of 2 times 1 ‰; Daily disease gets final product by spraying a thiophanate methyl of 750 times in per ten days.
10, transplant: with possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging the spring of Second Year, transplant by the seeding row spacing of 17 centimetres of 12 cm x.Follow-up management gets final product by existing planting type.
The above is only for the preferred embodiment of the invention; not in order to limit the invention; all in the invention spirit and principle within do any modification, be equal to and replace and improvement etc., all should be included within the protection domain of the invention.

Claims (7)

1. the method for the live stem of noble dendrobium seedling of plastic tunnel is characterized in that: may further comprise the steps:
Step 1, build booth: the highest 20000 luxs that are no more than of illumination in the booth;
Step 2, making seedbed: the seedbed is the built on stilts plastic deeping in bottom;
Step 3, matrix and sterilization: pine bark and sheep excrement are pressed (3~5): 1 mixes, and rotten thoroughly rear 3~5 purpose screen clothes of crossing of fermentation soaked 3~5 minutes with 600 times carbendazim, then pave on the seedbed, 8~12 centimetres of thickness were placed 5~10 days, and moisture control is at 60 ﹪~65 ﹪;
Step 4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 3~5 purpose screen clothes, by every square metre of consumption that spreads 1~1.5 kilogram of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly;
Step 5, seed preliminary treatment: the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 4~6 minutes, is then cut open, the seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine and infiltrate 4~6 hours;
Sterilize in step 6, the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
Step 7, broadcast sowing: turn at outside air temperature and begin sowing in spring after warm, also be 17~30 ℃ of outdoor temperatures, get 0.8~1.2 kilogram of matrix in the seedbed first, mix 8~12 seeds that soaked hormone, then be sprinkling upon on the seedbed after mixing thoroughly, area is 8~12 square metres;
Step 8, temperature, light, humidity control: before extremely transplanting after broadcasting sowing, temperature of shed is controlled between 18 ℃~30 ℃, the seedbed moisture control is at 60%~65 ﹪, air humidity maintains 80 ﹪~90 ﹪, the first month of broadcasting sowing, process with the low light level, maximum light intensity is no more than 2000 luxs, adds gradually intense light irradiation after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: keep seedbed moisture content 60%~65 ﹪ with sprinkling equipment, air humidity 80 ﹪~90 ﹪, behind two leaves of seed germination extraction, spray weekly a flower foliage fertilizer of 1 to 2 time 1 ‰, daily disease by sprayed in per ten days one time 600 times carbendazim or 750 times thiophanate methyl get final product;
Step 10, transplant: will possess complete, the stem of noble dendrobium seedling of stem, leaf the spring of Second Year, rise together with matrix one digging and transplant, follow-up management gets final product by normal planting type.
2. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, it is characterized in that: in the described step 1, described greenhouse is high 2.5~4 meters, and is wide 5~7 meters.
3. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 2 is characterized in that: the sunshade net that covers 6~9 thick plastic foils and sunshade rate 70 ﹪~90% above the booth.
4. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 2 or claim 3, it is characterized in that: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 1~2 meter, and length is as the criterion with canopy length.
5. the method for the live Dendrobidium huoshanness seedling of a kind of plastic tunnel as claimed in claim 1, it is characterized in that: when the live Dendrobidium huoshanness seedling of plastic tunnel, above-mentioned steps 1 to 10 is specially:
Step 1, build booth: greenhouse is high 3 meters, and is wide 6 meters, covers the sunshade net of 8 thick plastic foils and sunshade rate 90 ﹪ above the booth;
Step 2, making seedbed: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 1.5 meters, and length is as the criterion with canopy length;
Step 3, matrix and sterilization: pine bark and sheep excrement are pressed 4:1 mix, fermentation is rotten crosses 4 purpose screen clothes after thoroughly, soaks 4 minutes with 600 times carbendazim, then paves on the seedbed, and 10 centimetres of thickness were placed 7 days, controlled moisture content at 60 ﹪;
Step 4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 4 purpose screen clothes, by every square metre of consumption that spreads 1 kilogram of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly;
Step 5, seed preliminary treatment: the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 5 minutes, is then cut open, the seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine and infiltrate 5 hours;
Sterilize in step 6, the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
Step 7, broadcast sowing: begin sowing the spring that turns after warm at outside air temperature, also is outdoor temperature 17~25 degree, gets 1 kilogram of matrix in the seedbed first, mixes 10 seeds that soaked hormone, is sprinkling upon on the seedbed after then mixing thoroughly, and area is 10 square metres;
Step 8, temperature, light, humidity control: to transplanting, temperature of shed is controlled at 28 ℃ after broadcasting sowing, and the seedbed moisture control is at 60 ﹪, and air humidity maintains 80 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: keep seedbed moisture content 60 ﹪ with sprinkling equipment, air humidity 80 ﹪, after Dendrobidium huoshanness extracts two leaves out, spray weekly a flower foliage fertilizer of 1 time 1 ‰, daily disease is by spraying 600 times carbendazim in per ten days one time;
Step 10, transplanting: with possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging the spring of Second Year, transplant by the seeding row spacing of 10 centimetres of 10 cm x, follow-up management gets final product by normal planting type.
6. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, it is characterized in that: when the live HERBA DENDROBII seedling of plastic tunnel, above-mentioned steps 1 to 10 is specially:
Step 1, build booth: described greenhouse is high 2.5 meters, and is wide 5 meters, covers the sunshade net of 6 thick plastic foils and sunshade rate 70 ﹪ above the booth;
Step 2, making seedbed: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 1 meter, and length is as the criterion with canopy length;
Step 3, matrix and sterilization: pine bark and sheep excrement are pressed 3:1 mix, fermentation is rotten crosses 3 purpose screen clothes after thoroughly, with 600 times carbendazim immersions 5 minutes, then paves on the seedbed, and 8 centimetres of thickness were placed 5 days, and controlled humidity is at 65 ﹪;
Step 4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 3 purpose screen clothes, by every square metre of consumption that spreads 1.2 kilograms of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly;
Step 5, seed preliminary treatment: the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 4 minutes, is then cut open, the seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine and infiltrate 4 hours;
Sterilize in step 6, the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
Step 7, broadcast sowing: begin sowing the spring that turns after warm at outside air temperature, also is outdoor temperature 17~25 degree, gets 0.8 kilogram of matrix in the seedbed first, mixes 8 seeds that soaked hormone, is sprinkling upon on the seedbed after then mixing thoroughly, and area is 8 square metres;
Step 8, temperature, light, humidity control: to transplanting, temperature of shed is controlled at 18 ℃ after broadcasting sowing, and seedbed humidity is controlled at 65%, and air humidity maintains 70 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: keep seedbed humidity 65% with sprinkling equipment, air humidity 70 ﹪, wait the stem of noble dendrobium to extract two leaves out after, spray weekly a flower foliage fertilizer of 1 time 1 ‰; Daily disease is by spraying 750 times thiophanate methyl in per ten days one time;
Step 10, transplanting: with possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging the spring of Second Year, transplant by the seeding row spacing of 12 centimetres of 8 cm x, follow-up management is by existing planting type.
7. the method for the live stem of noble dendrobium seedling of a kind of plastic tunnel as claimed in claim 1, it is characterized in that: when the live dendrobium candidum seedling of plastic tunnel, above-mentioned steps 1 to 10 is specially:
Step 1, build booth: described greenhouse is high 4 meters, and is wide 7 meters, covers the sunshade net of 9 thick plastic foils and sunshade rate 90 ﹪ above the booth;
Step 2, making seedbed: plastic deeping rides on the fixed mount of angle steel welding, and built on stilts with hollow concrete block under the angle steel, and the seedbed is wide 2 meters, and length is as the criterion with canopy length;
Step 3, matrix and sterilization: pine bark and sheep excrement are pressed 5:1 mix, fermentation is rotten crosses 5 purpose screen clothes after thoroughly, with 600 times carbendazim immersions 3 minutes, then paves on the seedbed, and 12 centimetres of thickness were placed 10 days, and controlled humidity is at 62 ﹪;
Step 4, fungal component inoculation: get the old matrix of the cultivation stem of noble dendrobium more than 2 years, cross 5 purpose screen clothes, by every square metre of consumption that spreads 1.5 kilograms of old matrix, old matrix is sprinkling upon on the seedbed of completing new matrix uniformly;
Step 5, seed preliminary treatment: the stem of noble dendrobium outstanding achievement of maturation with the alcohol-pickled sterilization of 75 ﹪ 6 minutes, is then cut open, the seed in the fruit is poured on contains 2 Hao Ke ∕ and rise in the sterile water that methyl α-naphthyl acetate and 0.5 Hao Ke ∕ rise 6-benzyl aminopurine and infiltrate 5 hours;
Sterilize in step 6, the canopy: the guarantor mushroom king of every cubic metre of usefulness 4 grams is stifling, fumigates once before the sowing, and is stifling once every two weeks later on;
Step 7, broadcast sowing: begin sowing the spring that turns after warm at outside air temperature, also is outdoor temperature 17~25 degree, gets 1.2 kilograms of matrix in the seedbed first, mixes 12 seeds that soaked hormone, is sprinkling upon on the seedbed after then mixing thoroughly, and area is 12 square metres;
Step 8, temperature, light, humidity control: to transplanting, temperature of shed is controlled at 25 ℃ after broadcasting sowing, and seedbed humidity is controlled at 62%, and air humidity maintains 80 ﹪; The first month of broadcasting sowing, process with the low light level, and maximum intensity is no more than 2000 luxs, strengthens gradually illumination after sprouting, but the highest 20000 luxs that are no more than;
Step 9, water, fertilizer, plant disease management: keep seedbed humidity 62% with sprinkling equipment, air humidity 80 ﹪, wait the stem of noble dendrobium to extract two leaves out after, spray weekly a flower foliage fertilizer of 2 times 1 ‰; Daily disease is by spraying 750 times thiophanate methyl in per ten days one time;
Step 10, transplanting: with possessing the stem of noble dendrobium seedling of complete root, stem, leaf, rise together with matrix one digging the spring of Second Year, transplant by the seeding row spacing of 17 centimetres of 12 cm x, follow-up management is by existing planting type.
CN201310004397.7A 2013-01-06 2013-01-06 Method for directly seedling dendrobe seedlings by using plastic greenhouse CN103004445B (en)

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