CN115777375B - UV-A radiation method for improving active substance content of dendrobium nobile - Google Patents
UV-A radiation method for improving active substance content of dendrobium nobile Download PDFInfo
- Publication number
- CN115777375B CN115777375B CN202211651908.XA CN202211651908A CN115777375B CN 115777375 B CN115777375 B CN 115777375B CN 202211651908 A CN202211651908 A CN 202211651908A CN 115777375 B CN115777375 B CN 115777375B
- Authority
- CN
- China
- Prior art keywords
- content
- dendrobium
- weeks
- radiation
- total
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 230000005855 radiation Effects 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 23
- 239000013543 active substance Substances 0.000 title claims abstract description 21
- 240000004638 Dendrobium nobile Species 0.000 title claims description 54
- 241001523681 Dendrobium Species 0.000 claims abstract description 10
- 241001076416 Dendrobium tosaense Species 0.000 claims description 37
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 claims description 28
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 claims description 17
- 229930003944 flavone Natural products 0.000 claims description 17
- 150000002212 flavone derivatives Chemical group 0.000 claims description 17
- 235000011949 flavones Nutrition 0.000 claims description 17
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 claims description 17
- 238000005286 illumination Methods 0.000 claims description 13
- 150000004676 glycans Chemical class 0.000 abstract description 29
- 229920001282 polysaccharide Polymers 0.000 abstract description 29
- 239000005017 polysaccharide Substances 0.000 abstract description 29
- 229930003935 flavonoid Natural products 0.000 abstract description 17
- 150000002215 flavonoids Chemical class 0.000 abstract description 17
- 235000017173 flavonoids Nutrition 0.000 abstract description 17
- 150000002989 phenols Chemical class 0.000 abstract description 7
- 238000003306 harvesting Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 241000026010 Dendrobium candidum Species 0.000 description 20
- 230000000694 effects Effects 0.000 description 17
- 230000009469 supplementation Effects 0.000 description 10
- 230000012010 growth Effects 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 4
- 230000001502 supplementing effect Effects 0.000 description 4
- 241000208822 Lactuca Species 0.000 description 3
- 235000003228 Lactuca sativa Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 2
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 2
- 229930002877 anthocyanin Natural products 0.000 description 2
- 235000010208 anthocyanin Nutrition 0.000 description 2
- 239000004410 anthocyanin Substances 0.000 description 2
- 150000004636 anthocyanins Chemical class 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- VJQAFLAZRVKAKM-QQUHWDOBSA-N lactucin Chemical compound O[C@@H]1CC(C)=C2C(=O)C=C(CO)[C@@H]2[C@H]2OC(=O)C(=C)[C@@H]21 VJQAFLAZRVKAKM-QQUHWDOBSA-N 0.000 description 2
- VJQAFLAZRVKAKM-UHFFFAOYSA-N lactucine Natural products OC1CC(C)=C2C(=O)C=C(CO)C2C2OC(=O)C(=C)C21 VJQAFLAZRVKAKM-UHFFFAOYSA-N 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001669696 Butis Species 0.000 description 1
- RYAHJFGVOCZDEI-UFFNCVEVSA-N Dendrobine Chemical compound C([C@H]1CC[C@@H]2[C@@]31C)N(C)[C@@H]3[C@H]1[C@@H](C(C)C)[C@@H]2C(=O)O1 RYAHJFGVOCZDEI-UFFNCVEVSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- RYAHJFGVOCZDEI-CZKZLRAZSA-N dendrobine Natural products O=C1O[C@@H]2[C@H](C(C)C)[C@H]1[C@H]1[C@@]3(C)[C@@H]2N(C)C[C@H]3CC1 RYAHJFGVOCZDEI-CZKZLRAZSA-N 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B20/00—Energy efficient lighting technologies, e.g. halogen lamps or gas discharge lamps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The application provides Sup>A UV-A radiation method for improving the content of active substances of dendrobe, which is characterized in that the content of total flavonoids, total phenols and polysaccharide active substances before harvesting of mature dendrobe seedlings is improved by radiating dendrobe for 1-3 weeks through white light and UV-A, and the two dendrobe are subjected to UV-A radiation treatment with intensities of 365nm, 385nm and 395nm under Sup>A white light background, so that the difference of the total flavonoids, the total phenols and the polysaccharide at the stem and leaf radiation positions of the two dendrobe is compared. The result shows that the proper UV-A radiation can obviously improve the content of the total flavonoids, the total phenols and the polysaccharides of the dendrobium. The method can induce herbSup>A Dendrobii to synthesize more total flavonoids, total phenols and polysaccharides in Sup>A short period, and greatly improve commodity quality, thereby properly adjusting UV-A radiation intensity and period according to two kinds of herbSup>A Dendrobii production and harvesting requirements.
Description
Technical Field
The application belongs to the field of plant cultivation and facility research, and particularly relates to Sup>A UV-A radiation method for improving the content of active substances in dendrobium nobile.
Background
Dendrobium officinale (Dendrobium officinale) and dendrobium nobile (Dendrobium nobile) are plants of the genus Dendrobium of the family Orchidaceae, have ornamental and medicinal values, are precious traditional Chinese medicinal materials, are developed into various food materials and health medicinal materials in recent years, and are widely planted and applied by courtyards and simulated wild species. The main active substances of the two dendrobe include polysaccharide, total flavone, total phenol and the like, and have various effects of regulating immunity, resisting oxidation, resisting tumor, reducing blood sugar and the like, and in the cultivation process, the commodity quality and the extraction rate of the dendrobe are greatly improved by improving the content of the active substances. At present, the content of active substances of the dendrobium is mainly influenced by cultivation conditions and varieties, and the content of polysaccharide, dendrobine and the like of the dendrobium can be improved by the related light treatment, but the treatment time is longer, and the energy consumption is high.
UV-Sup>A is Sup>A wavelength of 315-400nm (1 nm=10 -9 m) of ultraviolet light in the region which is not absorbed efficiently by ozone, butIs also not harmful to the surface biosphere. According to Sup>A small amount of reports, the UV-A can regulate and control the active substance content of plants in Sup>A short period, and has low cost and quick response. Chinese patent CN113728904A discloses Sup>A molecular mechanism research method for accumulating anthocyanin in Chinese cabbage based on UV-A coupling, which uses UV-A coupling blue light with different light intensities to carry out light regulation treatment during growth so as to increase anthocyanin components rich in Chinese cabbage. Chinese patent CN114885770A discloses Sup>A lettuce quality control method based on UV-A illumination, which adopts wave crest 385nm and 15-45 mu mol.m before harvesting -2 ·s -1 The UV-A irradiation of 3-9d lettuce can obviously reduce the content of lettuce bitter substances (lactucin and lactucin) and obviously improve the content of total phenols and flavonoids. However, the influence of UV-A radiation on the active substance content of dendrobium is also reported.
Disclosure of Invention
Terminology and statement of the application:
in the present application, UV-A is ultraviolet light unless otherwise indicated; UV-B is blue light; w is white light; h is time unit hours; d is the time unit day; w is time unit week; mean is the average; CK is the control group.
Aiming at the problems existing in the prior art, the main system of the application researches the influence of UV-A radiation on the dry matter content, the content of main active substances of polysaccharide, total flavone, total phenol and the like of dendrobium candidum and dendrobium nobile, and designs the UV-A radiation before harvesting with convenient operation, light and simple energy consumption and obvious effect for the first time, and improves the content of the total flavone, the total phenol and the polysaccharide of dendrobium candidum to be 100.4%, 108.3% and 115.0% respectively at the highest; the highest content of the total flavonoids, the total phenols and the polysaccharides of the dendrobium nobile is respectively 156.7%, 146.6% and 131.5%, thereby greatly improving the quality and the commodity value of the dendrobium nobile and providing technical support for the production and the application of the dendrobium nobile.
In order to achieve the above purpose, the technical scheme adopted by the application is as follows:
the application provides Sup>A UV-A radiation method for improving the content of active substances in dendrobium, which comprises the step of radiating dendrobium for 1-3 weeks through UV-A T, wherein UV-A T is white light and UV-A.
Preferably, the dendrobium nobile comprises dendrobium candidum and dendrobium nobile, and the dendrobium nobile comprises stems and leaves at the radiation part.
Preferably, the stems include 1 year stems and 2 years stems.
Specifically, the 1-year stem is: the growth period of the plant reaches 1 year, namely, the time from the new bud to the treatment is 1 year; the 2-year stem is: the plant growth period is up to 2 years, i.e. 2 years from the time the sprout is treated.
Preferably, the active substance comprises at least one of flavone, phenol and polysaccharide.
Preferably, the UV-A T includes at least one of UV-A T1, UV-A T2 and UV-A T3;
wherein, the UV-A T1 is white light and 365nm UV-A, and the illumination is 12h/d; the UV-A T is white light and 385nm UV-A, and the illumination is carried out for 12h/d; the UV-A T is white light and 395nm UV-A, and the illumination is 12h/d.
The application also provides application of the UV-A radiation method in improving the content of active substances in dendrobium.
Preferably, the UV-A radiation method for improving the active substance content of the dendrobium candidum comprises the following steps of:
(1) Dendrobium officinale 1 year stem: UV-A T irradiation for 1-3 weeks;
(2) Dendrobium officinale 2 years old stem: UV-A T1 for 1-3 weeks or UV-A T2 for 1-3 weeks;
(3) Dendrobium officinale leaf: UV-A T irradiation for 1-3 weeks.
Further preferably, specifically, the UV-Sup>A radiation method for increasing the flavone content of dendrobium candidum comprises the following steps:
(1) Dendrobium officinale 1 year stem: UV-A T1 for 1 week, or UV-A T3 for 2 weeks; preferably UV-A T3 for 2 weeks;
(2) Dendrobium officinale 2 years old stem: UV-A T irradiation for 1-2 weeks; preferably UV-A T1 for 2 weeks;
(3) Dendrobium officinale leaf: UV-A T1 was irradiated for 3 weeks.
Wherein, the UV-A T1 radiation is as follows: UV-Sup>A parameters:
T1+UV-A365 nm, 12h/d illumination time, 6:00 in the morning-18:00 in the evening, and 1 week of radiation;
the UV-A T1 radiation for 3 weeks is: UV-Sup>A parameters:
T1+UV-A365 nm, 12h/d illumination time, 6:00 in the morning-18:00 in the evening, 3 weeks of radiation;
the UV-A T3 radiation for 2 weeks is: UV-Sup>A parameters:
T3+UV-A395 nm, light time 12h/d, 6:00 in the morning-18:00 in the evening, 2 weeks of irradiation.
Further preferably, the UV-A radiation method for improving the total phenol content of the dendrobium candidum comprises the following steps of:
(1) Dendrobium officinale 1 year stem: UV-A T1 is irradiated for 1-2 weeks, preferably UV-A T1 is irradiated for 2 weeks;
(2) Dendrobium officinale 2 years old stem: UV-A T2 irradiation for 2-3 weeks; preferably UV-A T2 for 3 weeks;
(3) Dendrobium officinale leaf: UV-A T1 for 2-3 weeks, or UV-A T3 for 1 week.
Specifically, the UV-A T2 radiation for 2-3 weeks is as follows: UV-Sup>A parameters:
W+UV-A385 nm, 12h/d of illumination time, 6:00 in the morning-18:00 in the evening, and 2-3 weeks of radiation;
further preferably, the UV-A radiation method for improving the polysaccharide content of dendrobium candidum comprises the following steps:
(1) Dendrobium officinale 1 year stem: UV-A T2 for 3 weeks, or UV-A T3 for 1 week, or UV-AT1 for 1-3 weeks; preferably UV-A T2 for 3 weeks;
(2) Dendrobium officinale 2 years old stem: UV-A T1 for 3 weeks, or UV-A T2 for 2 weeks; preferably UV-A T1 for 3 weeks;
(3) Dendrobium officinale leaf: UV-A T1 for 2 weeks, or UV-A T2, T3 for 1 week, or UV-AT2 for 3 weeks; preferably UV-A T2 for 3 weeks.
Preferably, the UV-A radiation method for improving the active substance content of dendrobium nobile comprises the following steps:
(1) Dendrobium nobile stem for 1 year: UV-A T for 3 weeks or UV-A T2 for 2-3 weeks;
(2) Dendrobium nobile stem for 2 years: UV-A T irradiation for 2-3 weeks;
(3) Dendrobium nobile leaf: UV-A T for 1 week or UV-A T for 1-2 weeks.
Further preferably, the UV-A radiation method for improving the total flavone content of dendrobium nobile comprises the following steps:
(1) Dendrobium nobile stem for 1 year: UV-A T2 irradiation for 2-3 weeks; preferably UV-A T2 for 3 weeks;
(2) Dendrobium nobile stem for 2 years: UV-A T2 irradiation for 2-3 weeks; preferably UV-A T2 for 3 weeks;
(3) Dendrobium nobile leaf: UV-A T radiation for 1-2 weeks; preferably UV-A T3 for 2 weeks.
Further preferably, the UV-A radiation method for improving the total phenol content of dendrobium nobile comprises the following steps:
(1) Dendrobium nobile stem 1 year and stem 2 years: UV-A T2 radiation for 3 weeks;
(2) Dendrobium nobile leaf: UV-A T was irradiated for 1 week.
Further preferably, the UV-A radiation method for improving the polysaccharide content of dendrobium nobile comprises the following steps:
(1) Dendrobium nobile stem for 1 year: UV-A T1 irradiation for 3 weeks;
(2) Dendrobium nobile stem for 2 years: UV-A T1 or T2 or T3 irradiation for 2 weeks; preferably UV-A T1 or T3 radiation for 2 weeks;
(3) Dendrobium nobile leaf: UV-A T2 was irradiated for 1 week.
Compared with the prior art, the application has the following beneficial effects:
(1) Compared with other light treatment, the UV-A treatment period is shorter, the UV-A treatment method can achieve better effect in shorter time, and the production cost is lower;
(2) The application greatly improves the contents of total flavonoids, total phenols and polysaccharides of dendrobium candidum and dendrobium nobile, and the improvement range is larger.
Drawings
FIG. 1 is Sup>A graph showing the growth of Dendrobium officinale irradiated with UV-A for 3 weeks in example 1 (T1: W+365nm UV-A; T2: W+385nm UV-A; T3: W+395nm UV-A; CK: W);
FIG. 2 is Sup>A graph showing the growth of Dendrobium nobile (T1: W+365nm UV-A; T2: W+385nm UV-A; T3: W+395nm UV-A; CK: W) irradiated with UV-A for 3 weeks in example 2;
FIG. 3 shows the growth environment of Dendrobium officinale and Dendrobium nobile in white light combined with UV-A light supplementing experiment (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W);
FIG. 4 is Sup>A bar graph showing the effect of UV-A light supplementation on total flavonoids content of 1 year old stem of Dendrobium officinale in example 1 (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W);
FIG. 5 is Sup>A bar graph showing the effect of UV-A light supplementation on total flavonoids content of 2-year stems of Dendrobium officinale in example 1 (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W);
FIG. 6 is Sup>A bar graph showing the effect of UV-A light supplementation on total flavonoids content of Dendrobium officinale leaves in example 1 (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W);
FIG. 7 is Sup>A bar graph showing the effect of UV-A light supplementation on total flavonoids content of 1 year old stem of Dendrobium nobile in example 2 (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W);
FIG. 8 is Sup>A bar graph showing the effect of UV-A light supplementation on total flavonoids content of 2-year stems of Dendrobium nobile in example 2 (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W);
FIG. 9 is Sup>A bar graph showing the effect of UV-A light supplementation on the total flavonoids content of dendrobium nobile leaves in example 2 (T1:W+365 nm UV-A; T2:W+385nm UV-A; T3:W+395nm UV-A; CK: W).
Detailed Description
It is to be noted that the raw materials used in the present application are all common commercial products, and the sources thereof are not particularly limited.
EXAMPLE 1 Effect of UV-A radiation on Dendrobium officinale active content
Step 1-1, dendrobium candidum radiation environment: placing mature seedlings capped by dendrobium candidum leaves in an LED greenhouse, wherein the indoor average temperature is about 25 ℃, adopting white light combined with 365-395nm UV-A light supplementing treatment, adopting light intensity of 365nm, 385nm and 395nm wavelengths, and adopting white light treatment in contrast, wherein the light intensity of the white light is 40+/-1 mu mol.m -2 ·s -1 The humidity of the greenhouse is 65-70%.
Step 1-2, radiation time: the light was illuminated for 12 hours per day from 6:00 a.m. to 18:00 a.m.. The induction time was 3w.
Step 1-3, quality test: the dry matter content of the 1 year stems, the 2 year stems and the 1 year leaves of the dendrobium candidum are measured by treating 1w, 2w and 3w respectively, and the polysaccharide, the total flavone and the total phenol content are repeated for 3 times.
Tables 1-2 show the effect of UV-A radiation on total phenol and polysaccharide content of Dendrobium officinale growth in example 1.
FIGS. 4-5 are bar graphs showing the effect of UV-A light supplementation on total flavonoids content of 1-year and 2-year stems of Dendrobium officinale in example 1, respectively; FIG. 6 is Sup>A bar graph showing the effect of UV-A light supplementation on the total flavonoids content of Dendrobium officinale leaves in example 1.
TABLE 1 influence of UV-A radiation on the total phenol content of Dendrobium officinale (mg/g)
| Total phenol content of 1 year stem | 1w | 2w | 3w | mean |
| T1 | 10.46 | 14.26 | 10.53 | 11.75a |
| T2 | 9.51 | 8.35 | 9.13 | 9.00b |
| T3 | 9.44 | 7.81 | 7.75 | 8.33b |
| CK | 9.53 | 7.59 | 8.87 | 8.66b |
| mean | 9.73a | 9.50a | 9.07b | |
| Total phenol content of 2 years stem | ||||
| T1 | 14.61 | 10.41 | 8.48 | 11.17d |
| T2 | 13.82 | 15.21 | 17.40 | 15.48a |
| T3 | 14.61 | 9.95 | 14.92 | 13.16c |
| CK | 18.67 | 13.53 | 14.12 | 15.44b |
| mean | 15.43a | 12.28c | 13.73b | |
| Total phenol content of leaves | ||||
| T1 | 30.38 | 52.43 | 58.70 | 50.64a |
| T2 | 24.73 | 28.28 | 26.02 | 26.35d |
| T3 | 40.78 | 27.11 | 32.95 | 30.15c |
| CK | 27.48 | 36.93 | 28.18 | 30.86b |
| 30.84b | 36.19a | 36.46a |
TABLE 2 influence of UV-A radiation on the polysaccharide content of Dendrobium officinale (%)
| 1 year stem polysaccharide content | 1w | 2w | 3w | mean |
| T1 | 25.29 | 35.00 | 19.53 | 26.61b |
| T2 | 25.25 | 28.17 | 30.33 | 27.92a |
| T3 | 30.18 | 9.91 | 17.02 | 19.04d |
| CK | 22.61 | 31.94 | 14.11 | 22.87c |
| mean | 25.83a | 26.26a | 20.25b | |
| 2 years stem polysaccharide content | ||||
| T1 | 29.19 | 31.97 | 25.68 | 28.95a |
| T2 | 22.99 | 33.63 | 22.37 | 26.33b |
| T3 | 26.53 | 25.26 | 14.31 | 22.03c |
| CK | 28.69 | 29.82 | 20.12 | 26.21b |
| mean | 26.85a | 30.17b | 20.62c | |
| Leaf polysaccharide content | ||||
| T1 | 14.64 | 35.04 | 7.18 | 18.95b |
| T2 | 22.15 | 16.78 | 23.20 | 20.71a |
| T3 | 21.39 | 17.75 | 11.65 | 16.93c |
| CK | 16.35 | 21.91 | 13.68 | 17.31c |
| 18.63b | 22.87a | 13.93c |
The dendrobium candidum is irradiated with UV-A T3 for 2 weeks or UV-A T for 3 weeks, so that the total flavone content of the 1-year stems and leaves of the dendrobium candidum is obviously increased. Wherein the total flavone content of the 1-year stem of the dendrobium candidum is obviously improved by 100.4 percent and 43.1 percent respectively after being irradiated by UV-A T3 for 2 weeks or irradiated by UV-A T1 for 1 week; after being irradiated by UV-A T for 3 weeks, the total flavone content of the dendrobium candidum leaves is obviously improved by 95.5%. After UV-A T1 radiation for 2 weeks, the total flavone content of the 2-year stem of Dendrobium officinale is promoted to be obviously improved by 12.7%.
The total phenol content of the stems and leaves is obviously increased after being irradiated by UV-A T1 for 1-2 weeks or irradiated by UV-A for 3 weeks respectively for 1 year; wherein the total phenol content of the 1-year stem of the dendrobium candidum is obviously improved by 87.9 percent after being irradiated by UV-A T1 for 2 weeks; the total phenol content of the dendrobium candidum leaves is obviously improved by 42.0-108.3% or 48.4% by UV-A T1 irradiation for 2-3 weeks or T3 irradiation for 1 week; after UV-A T2 radiation for 2-3 weeks, the total phenol content of the 2-year stem of Dendrobium officinale is obviously improved by 12.4-23.1%.
UV-A T1 low-radiation treatment for 3 weeks or T2 radiation for 3 weeks obviously promotes the increase of the stem and leaf polysaccharide content of the dendrobium candidum. Wherein UV-A T2 is radiated for 3 weeks, or radiated for 1 week by T3, or radiated for 3 weeks by T1, the polysaccharide content of the dendrobium candidum 1 year stem is obviously improved by 115.0%, 33.5% and 38.4% respectively; UV-AT1 radiation for 3 weeks or T2 radiation for 2 weeks, and the polysaccharide content of the 2-year stems of Dendrobium officinale is obviously improved by 27.6% and 12.8% respectively; UV-A T1 radiation for 2 weeks and UV-A T radiation for 1 week and 3 weeks respectively promote the polysaccharide content of dendrobium candidum leaves to be obviously improved by 59.9%, 35.5% and 69.6%.
Example 2 Effect of UV-A radiation on the active content of Dendrobium nobile
Step 1-1, dendrobium nobile radiation environment: the mature seedling capped by dendrobium nobile leaves is placed in an LED greenhouse, the indoor average temperature is about 25 ℃, white light is combined with 365-395nm UV-A light supplementing treatment, the light intensity of 365nm, 385nm and 395nm wavelengths is adopted, the contrast is treated by white light, and the light intensity of the white light is 40+/-1 mu mol.m -2 ·s -1 The humidity of the greenhouse is 65-70%.
Step 1-2, radiation time: the light was illuminated for 12 hours per day from 6:00 a.m. to 18:00 a.m.. The induction time was 3w.
Step 1-3, quality test: the dry matter contents of the stem of dendrobium nobile of 1 year, the stem of 2 years and the leaf of 1 year are respectively measured in the treatment of 1w, 2w and 3w, and the polysaccharide, the total flavone and the total phenol content are repeated for 3 times.
Tables 3-4 show the effect of UV-A radiation on total phenol and polysaccharide content of dendrobium nobile growth in example 2.
FIGS. 7-8 are bar graphs showing the effect of UV-A light supplementation on total flavonoids content of stem of Dendrobium nobile for 1 year and 2 years in example 2, respectively; FIG. 9 is Sup>A bar graph showing the effect of UV-A light supplementation on the total flavonoids content of Dendrobium nobile leaves in example 2.
FIG. 3 shows the growth environment of Dendrobium officinale and Dendrobium nobile in white light combined with UV-A light supplementing experiment (T1:W+UV-A365 nm; T2:W+UV-A385 nm; T3:W+UV-A365 nm; CK: white light W).
TABLE 3 influence of UV-A radiation on the total phenol content of Dendrobium nobile (mg/g)
| Total phenol content of 1 year stem | 1w | 2w | 3w | mean |
| T1 | 27.68 | 17.85 | 31.97 | 25.83c |
| T2 | 24.07 | 27.72 | 41.06 | 30.95a |
| T3 | 33.74 | 18.84 | 27.96 | 26.85b |
| CK | 23.61 | 25.98 | 16.65 | 22.08d |
| mean | 27.28b | 22.60c | 29.41a | |
| Total phenol content of 2 years stem | ||||
| T1 | 26.62 | 25.10 | 28.85 | 26.86b |
| T2 | 24.28 | 31.26 | 45.62 | 33.72a |
| T3 | 32.46 | 28.12 | 24.30 | 28.29c |
| CK | 32.42 | 31.19 | 38.60 | 34.01a |
| mean | 28.94b | 28.92b | 34.34a | |
| Total phenol content of leaves | ||||
| T1 | 54.53 | 57.47 | 44.57 | 52.19b |
| T2 | 47.50 | 39.03 | 45.29 | 43.94c |
| T3 | 61.00 | 53.06 | 53.13 | 55.73a |
| CK | 53.75 | 52.46 | 57.96 | 54.72a |
| 54.20a | 50.50b | 50.24b |
TABLE 4 influence of UV-A radiation on the polysaccharide content of Dendrobium nobile (%)
The total flavone content of the stem harvesting part of the dendrobium nobile is obviously promoted to be increased by the radiation treatment in the UV-A T for 2 weeks, and the influence on leaves is not obvious. Wherein UV-A T2 irradiation is carried out for 2-3 weeks to promote the total flavone content of stem of Dendrobium nobile for 1 year or 2 years to be respectively increased by 19.2-156.7% and 12.2-35.6%; UV-A T is radiated for 1-2 weeks, so that the total flavone content of dendrobium nobile leaves is obviously improved by 15.2-19.2%.
The total phenol content of the harvesting part of the stem of the dendrobium nobile is obviously promoted to be increased by the radiation treatment in the UV-A T for 3 weeks, but the total phenol content of the leaves is obviously reduced. Wherein UV-A T is radiated for 3 weeks to respectively promote the total phenol content of 1-year stems and 2-year stems of dendrobium nobile to be improved by 146.6 percent and 18.2 percent; UV-A T is radiated for 1 week, so that the total phenol content of dendrobium nobile leaves is obviously improved by 13.5%.
After UV-A T1 low radiation for 3 weeks, the polysaccharide content of the stem of dendrobium nobile for 1 year is obviously improved by 18.3%; after UV-A T1, T2 and T3 are radiated for 2 weeks, the polysaccharide content of the stem of the dendrobium nobile for 2 years is promoted to be respectively improved by 92.8 percent, 32.3 percent and 102.1 percent; UV-A T is radiated for 1 week, so that the polysaccharide content of dendrobium nobile leaves is improved by 131.5%.
Finally, it should be noted that the above description is only for illustrating the technical solution of the present application, and not for limiting the scope of the present application, and that the simple modification and equivalent substitution of the technical solution of the present application can be made by those skilled in the art without departing from the spirit and scope of the technical solution of the present application.
Claims (3)
1. A UV-A radiation method for improving the content of active substances in dendrobium is characterized by comprising the following steps of: the UV-A comprises at least one of UV-A T1, UV-A T2 and UV-A T3; wherein, the UV-A T1 is white light and 365nm UV-A, and the illumination is 12h/d; the UV-A T is white light and 385nm UV-A, and the illumination is carried out for 12h/d; the UV-A T is white light and 395nm UV-A, and the illumination is carried out for 12h/d; the dendrobium nobile is of the type Dendrobium officinale, the active substance is flavone, and the UV-A radiation method for improving the flavone content of the Dendrobium officinale comprises the following steps:
(1) Dendrobium officinale 1 year stem: UV-A T radiation for 2 weeks;
(2) Dendrobium officinale leaf: UV-A T1 was irradiated for 3 weeks.
2. Sup>A method for radiating UV-A for improving the active substance content of dendrobium, which is characterized in that the UV-A comprises at least one of UV-A T1, UV-A T2 and UV-A T3; wherein, the UV-A T1 is white light and 365nm UV-A, and the illumination is 12h/d; the UV-A T is white light and 385nm UV-A, and the illumination is carried out for 12h/d; the UV-A T is white light and 395nm UV-A, and the illumination is carried out for 12h/d; the dendrobium nobile is of the type Dendrobium officinale, the active substance is phenol, and the UV-A radiation method for improving the phenol content of the Dendrobium officinale comprises the following steps:
(1) Dendrobium officinale 1 year stem: UV-A T1 irradiation for 2 weeks;
(2) Dendrobium officinale leaf: UV-A T1 was irradiated for 3 weeks.
3. Use of the UV-Sup>A radiation method according to any one of claims 1-2 for increasing the active substance content of dendrobe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211651908.XA CN115777375B (en) | 2022-12-21 | 2022-12-21 | UV-A radiation method for improving active substance content of dendrobium nobile |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211651908.XA CN115777375B (en) | 2022-12-21 | 2022-12-21 | UV-A radiation method for improving active substance content of dendrobium nobile |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115777375A CN115777375A (en) | 2023-03-14 |
| CN115777375B true CN115777375B (en) | 2023-10-24 |
Family
ID=85427682
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211651908.XA Active CN115777375B (en) | 2022-12-21 | 2022-12-21 | UV-A radiation method for improving active substance content of dendrobium nobile |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115777375B (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704035A (en) * | 2013-12-27 | 2014-04-09 | 广东药学院 | Cultivation method for improving polysaccharide ingredients and phenol ingredients of dendrobium officinale |
| CN204652717U (en) * | 2015-02-13 | 2015-09-16 | 张辉 | A kind of LED food illuminator |
| CN109997622A (en) * | 2019-04-17 | 2019-07-12 | 广州市八斗农业科技有限公司 | The method for improving dendrobium candidum active constituent content |
| CN110769684A (en) * | 2018-04-20 | 2020-02-07 | 忠北国立大学产学合作基金会 | Plant cultivation method using UV and plant cultivation system therefor |
| CN111182786A (en) * | 2018-08-24 | 2020-05-19 | 首尔伟傲世有限公司 | Light source for plant cultivation |
| CN113141905A (en) * | 2020-12-29 | 2021-07-23 | 中国农业科学院都市农业研究所 | Method for improving quality of facility Chinese cabbage before harvesting |
| CN114885770A (en) * | 2022-05-26 | 2022-08-12 | 上海交通大学 | Lettuce quality regulation and control method based on UV-A illumination |
| CN115250852A (en) * | 2021-12-03 | 2022-11-01 | 浙江农林大学 | Method for promoting accumulation of flavonoid compounds in radix tetrastigme |
-
2022
- 2022-12-21 CN CN202211651908.XA patent/CN115777375B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704035A (en) * | 2013-12-27 | 2014-04-09 | 广东药学院 | Cultivation method for improving polysaccharide ingredients and phenol ingredients of dendrobium officinale |
| CN204652717U (en) * | 2015-02-13 | 2015-09-16 | 张辉 | A kind of LED food illuminator |
| CN110769684A (en) * | 2018-04-20 | 2020-02-07 | 忠北国立大学产学合作基金会 | Plant cultivation method using UV and plant cultivation system therefor |
| CN111182786A (en) * | 2018-08-24 | 2020-05-19 | 首尔伟傲世有限公司 | Light source for plant cultivation |
| CN109997622A (en) * | 2019-04-17 | 2019-07-12 | 广州市八斗农业科技有限公司 | The method for improving dendrobium candidum active constituent content |
| CN113141905A (en) * | 2020-12-29 | 2021-07-23 | 中国农业科学院都市农业研究所 | Method for improving quality of facility Chinese cabbage before harvesting |
| CN115250852A (en) * | 2021-12-03 | 2022-11-01 | 浙江农林大学 | Method for promoting accumulation of flavonoid compounds in radix tetrastigme |
| CN114885770A (en) * | 2022-05-26 | 2022-08-12 | 上海交通大学 | Lettuce quality regulation and control method based on UV-A illumination |
Non-Patent Citations (7)
| Title |
|---|
| UV-B辐射对铁皮石斛生长及主要次生代谢产物的影响;郭玉梅;曾令杰;梁淑颖;邓荣乾;叶惠娜;廖素溪;;北方园艺(第17期);全文 * |
| 不同LED光质对迭鞘石斛生理及品质的影响;李婷婷;淳泽;罗傲雪;向泓波;郭龙;张立;陈斯天;范益军;;应用与环境生物学报(第05期);全文 * |
| 不同氮素水平对铁皮石斛UV-B辐射缓解作用研究;崔丹丹;曾令杰;莫运才;张晓元;冯凯;;西南师范大学学报(自然科学版)(第04期);全文 * |
| 光酶诱导技术增加植物活性成分的研究进展;马鲁豫;陈润泽;崔磊;张琳;田景奎;;中国药学杂志(第14期);全文 * |
| 刘伟超.知网.2022,对比文件"不同光质对叠鞘石斛和铁皮石斛生长发育的影响"，第37-50页. * |
| 德宏州石斛的人工规范化栽培技术;番绍玲;;云南农业科技(第03期);全文 * |
| 石斛属植物光合碳同化途径研究综述;任建武;杨丽娜;王兴枝;;中国园艺文摘(第10期);全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115777375A (en) | 2023-03-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Goto | Plant production in a closed plant factory with artificial lighting | |
| US11606912B2 (en) | Method for increasing amount of phenolic compound in plant | |
| Ahmadi et al. | LED light mediates phenolic accumulation and enhances antioxidant activity in Melissa officinalis L. under drought stress condition | |
| Li et al. | Effect of supplemental blue light intensity on the growth and quality of Chinese kale | |
| CN111820130B (en) | Potato microtuber induction method based on LED energy-saving light source | |
| Li et al. | Effects of yellow, green, and different blue spectra on growth of potato plantlets in vitro | |
| Enache et al. | Preliminary results regarding the testing of treatments with light-emitting diode (LED) on the seed germination of Artemisia dracunculus L. | |
| CN106212070B (en) | Method for promoting rice seedling raising by using LED delayed supplementary lighting | |
| CN111512819A (en) | Method for increasing content of nutrient components of red beet by blue-violet light coupling treatment | |
| CN115777375B (en) | UV-A radiation method for improving active substance content of dendrobium nobile | |
| Huang et al. | Metabolomics and transcriptomics analysis of vitro growth in pitaya plantlets with different LED Light spectra treatment | |
| CN103907532A (en) | Heracleum moellendorffii callus induction method | |
| CN110915468A (en) | Method for increasing glucosinolate content of broccoli sprouting vegetable | |
| Gherghina et al. | Biochemical changes under artificial LED lighting in some Lactuca sativa L. varieties. | |
| Lim et al. | Effect of different LED lights spectrum on the'in vitro'germination of gac seed'(Momordica cochinchinensis)' | |
| KR20200012127A (en) | Method for improving growth and phytochemicals of perilla leaf using Quantum Dot lighting | |
| CN107155888A (en) | A kind of method that Camellia nitidissima embryo tissue-cultured seedling is cultivated under sunlight conditions | |
| CN105594597B (en) | V. amurensis tissue culture medium (TCM) and V. amurensis method for tissue culture | |
| Chen et al. | The effects of fixation methods on the composition and activity of sea buckthorn (Hippophae rhamnoides L.) leaf tea | |
| CN114009339B (en) | Method for inducing germination by using leaves of litsea cubeba | |
| CN114885770B (en) | Lettuce quality regulation and control method based on UV-A illumination | |
| CN113455366B (en) | Cultivation method for reducing bitter taste of lettuce and improving quality of lettuce | |
| CN114885770A (en) | Lettuce quality regulation and control method based on UV-A illumination | |
| Böhme et al. | Growth and internal quality of Vietnamese coriander (Polygonum odoratum Lour.) affected by additional lighting with blue, red and green LEDs | |
| JP5906085B2 (en) | Method for improving content of useful components in plants in hydroponics |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |