CN106718755A - A kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content - Google Patents

A kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content Download PDF

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Publication number
CN106718755A
CN106718755A CN201611228045.XA CN201611228045A CN106718755A CN 106718755 A CN106718755 A CN 106718755A CN 201611228045 A CN201611228045 A CN 201611228045A CN 106718755 A CN106718755 A CN 106718755A
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CN
China
Prior art keywords
light source
led light
chinese cabbage
cultural
nitrate content
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CN201611228045.XA
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Chinese (zh)
Inventor
樊小雪
陈龙正
徐海
宋波
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江苏省农业科学院
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Priority to CN201611228045.XA priority Critical patent/CN106718755A/en
Publication of CN106718755A publication Critical patent/CN106718755A/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

Abstract

The cultural method of Chinese cabbage nitrate content is quickly effectively reduced the invention discloses a kind of utilization LED light source, is comprised the following steps:(1), the preparation of seedling:Chinese cabbage seed soaks in being first put into clear water, places into the sponge seedling growing block of clear water immersion, after seedling two panels true leaf is fully deployed, water planting is carried out in the hydroponic box that seedling immigration is filled nutrient solution;(2), LED light source treatment:When Chinese cabbage grows to 8~16 true leaves, processed 10~48 hours under continuous illumination under LED light source, 22~25 DEG C of day temperature, 18~20 DEG C of nocturnal temperature, light intensity is set to 100~150 μm of olm‑2s‑1, relative humidity control is 60%~80%.The inventive method improves the quality of Chinese cabbage, and nitrate content quickly can be effectively reduced in a short time, improves security quality, save energy environmental protection.

Description

A kind of utilization LED light source quickly effectively reduces the cultivation of Chinese cabbage nitrate content Culture method
Technical field
The invention belongs to biological technical field, it is related to one kind quickly effectively to reduce Chinese cabbage nitric acid using LED light source The cultivation technique of salt content.
Background technology
Vegetables are indispensable plant food in people's daily life, its various essential amino acid for the mankind provide And vitamin, mineral matter and the cellulose for enriching.Meanwhile, vegetables are also a kind of nitrate content crop higher, are the mankind The main source of nitrate is absorbed, especially stem leafy vegetable.It is happiness nitre class plant because leaf vegetables is most, under nitrate nitrogen Maximum production is likely to be breached, along with the limitation of low light environment in facility cultivation, therefore is easier to cause the accumulation of nitrate.Food Nitrate content in thing is always the problem that people extremely pay close attention to, especially vegetables, and the height of nitrate content is more counted as It is an important indicator of vegetables quality.With the improvement of people ' s living standards, influence of the NO3-N and NO2-N to human body Increasingly it is taken seriously.If the accumulation of nitrate from vegetables self-view, can be reduced, utilization of the vegetables to nitrate is improved Rate, then be of great importance to vegetables production, human health and utilization rate of nitrogen fertilizer.Therefore, exploring efficiently reduces vegetables nitric acid The regulatory pathway and its mechanism of salt accumulation, for improving China's quality of vegetable, ensure food safety, and safeguard that common people's health has ten Divide important meaning.
Green vegetables (Brassica campestris ssp.Chinensis Makino) also known as Chinese cabbage, pakchoi Deng, it is northern to claim rape, it is a kind of staple vegetable of China middle and lower reach of Yangtze River and its areas to the south, substantially it is produced as with autumn It is main, can be with whole year production, year-round supply.Green vegetables are generally planted in the whole nation nearly ten years, and some seasons turn into the main of locality Vegetables.In recent years, extensive introducing and planting all over the world, has been increasingly becoming worldwide vegetable, with highly important economical and Social benefit.Therefore, the research of green vegetables has caused the great attention of lot of domestic and foreign scholar.
The content of the invention
It is an object of the invention to Reasonable Regulation And Control facility luminous environment, there is provided one kind is quickly effectively reduced using LED light source The cultivation technique of Chinese cabbage nitrate content.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content, and length is had into 8~16 The soilless culture under continuous illumination under LED light source of the Chinese cabbage of piece true leaf is processed 10~48 hours.
Utilization LED light source of the present invention quickly effectively reduces the cultural method of Chinese cabbage nitrate content, tool Body is comprised the following steps:
(1), the preparation of seedling:Chinese cabbage seed soaks 6~10 hours in being first put into clear water, places into clear water immersion Sponge seedling growing block in nursery, after seedling two panels true leaf is fully deployed, seedling is moved into be filled and enter in the hydroponic box of nutrient solution Water-filling is trained;Wherein, rearing condition is:Under the conditions of natural lighting, 20 ± 2 DEG C of temperature, photoperiod 12hd-1, relative humidity control 80%~90%;Water planting condition is:Under the conditions of natural lighting, 20 ± 2 DEG C of temperature, photoperiod 12hd-1, relative humidity control 80%~90%.
(2), LED light source treatment:When Chinese cabbage grows to 8~16 true leaves, locate under continuous illumination under LED light source Reason 10~48 hours, 22~25 DEG C of day temperature, 18~20 DEG C of nocturnal temperature, light intensity is set to 100~150 μm of olm-2·s-1, relative humidity control is 60%~80%.
In step (1), described nutrient solution is Huo Gelande nutrient solutions.Nutrient solution usage amount is wanted without special in the present invention Ask, as long as ensureing that nutrition supply can meet growth demand;Can ensure that Nitrogen supplying is uniform and numerical value is smart using water planting Really, it is more accurate than solid matrix, so as to reduce nitrate content in Chinese cabbage.
In step (2), Chinese cabbage grows to 10 true leaf edibles and ensures certain yield, it is therefore preferable that not tying When ball Chinese cabbage grows to 10 true leaves, processed under continuous illumination under LED light source.
Described LED light source is white light (W), monochromatic feux rouges (R), ultra-blue-light (B) or red blue 4:1 combination light (RB=4: 1), preferably ultra-blue-light (B) or red blue 4:1 combination light, more preferably red blue 4:1 combination light.
Preferably, processed 10~24 hours under continuous illumination under LED light source.
It is further preferred that being processed 24 hours under continuous illumination under LED light source.
LED light source of the present invention is by LED light lamp and drives adjuster to control light intensity and electric current.Can be with The composition of lamp is adjusted according to different light source requirements.
Compared to the prior art, beneficial effects of the present invention:
The invention provides a kind of method of utilization light source regulation and control control Chinese cabbage nitrate content, Chinese cabbage is improve Quality, with advantages below:1) test effect stabilization, clear, plant strain growth stalwartness, quickly can effectively drop in a short time Protonitrate content, improves security quality;2) simple to operate, effect is good;3) save energy environmental protection.
The inventive method is new for the development biological study relevant with Chinese cabbage facility cultivation and exploitation are provided Approach and foundation.
Brief description of the drawings
Fig. 1 is the energy efficiency indexes of different LED light source treatment;
Fig. 2 is that different LED light source continuous illuminations process 48 hours Chinese cabbage blade nitrate content variation tendencies;
Fig. 3 is that different LED light source continuous illuminations process 48 hours Chinese cabbage blade content of nitrite variation tendencies;
Fig. 4 is that different LED light sources process the influence to Chinese cabbage chlorophyll content in 24 hours.
Specific embodiment
Technical scheme is further described below by specific embodiment.Method therefor in embodiment Conventional method is unless otherwise instructed, and the percentage composition is volumn concentration unless otherwise instructed.
1st, first to the treatment of Chinese cabbage April slow-motion row LED light source, step is as follows:
(1) preparation of seedling:Chinese cabbage seed soaks 8 hours in being put into clear water, and the sponge for being put into clear water immersion educates Nursery in seedling block, environmental condition is as follows:Under the conditions of natural lighting, 20 ± 2 DEG C of temperature, photoperiod 12hd-1, relative humidity control 80%~90%;After seedling two panels true leaf is fully deployed, seedling is moved into filling the hydroponic box of Huo Gelande nutrient solutions Water planting is carried out, water planting condition is identical with rearing condition.
(2) LED light source treatment:When Chinese cabbage grows to 10 true leaves, choose the consistent seedling of form growing way and enter Row photo-irradiation treatment.Chinese cabbage is processed 48 hours under continuous illumination under LED light source, 22~25 DEG C of day temperature, night temperature 18~20 DEG C of degree, light intensity is set to 120 μm of olm-2·s-1, relative humidity control is 60%~80%.
2nd, light source:LED light source is white light (W), monochromatic feux rouges (R), ultra-blue-light (B) and red blue 4:1 combination light (RB=4: 1) it is, control (CK) with before processing.LED light source is to control light intensity and electricity by LED light lamp and a driving adjuster Stream.The composition of lamp can be adjusted according to different light source requirements.
3rd, treatment effect:
3.1 continuous illuminations process 48 hours Chinese cabbage blade nitrate content variation tendencies
See Fig. 2, result of the test finds, after continuous illumination is processed, monolithic white light, monochromatic feux rouges, ultra-blue-light With red blue 4:Nitrate content starts to the equal rapid decreases of 10h since treatment under 1 group of light combination process, continues decline since the 10h, There is a notable low spot by 24h hours, continue illumination nitrate content and raise on the contrary, but 4 ascendant trends for the treatment of are not Together.Red blue 4:Nitrate content is significantly lower than the nitric acid under other three groups of LED light source treatment of the same period all the time under 1 group of light combination process Salt content, the lower nitrate content of monolithic white light treatment is consistently higher than the nitrate content under other three groups of LED light source treatment of the same period. Ultra-blue-light, red blue 4:Nitrate content is below monolithic white light, monochromatic feux rouges treatment in 10h-48h under 1 group of light combination process, and The nitrate content minimum of 24h is significantly lower than monolithic white light, the minimum of monochromatic feux rouges treatment, particularly red blue 4:1 group Nitrate content is not higher than 25.5 μ g/g in 10h~48h under light combination process, minimum substantially less than to locate to 13.4 μ g/g or so Nitrate content (50 μ g/g) before reason.
3.2 continuous illuminations process 48 hours Chinese cabbage blade content of nitrite variation tendencies
See Fig. 3, monolithic white light, monochromatic feux rouges, ultra-blue-light and red blue 4:The nitrous of Chinese cabbage under 1 group of light combination process Phosphate content in different trend change, under the treatment of 4 LED light sources, under content of nitrite is quick to 10h since the treatment Drop, continuous processing is after 10 hours, when monolithic white light, the content of nitrite of the monochromatic lower Chinese cabbage of feux rouges treatment are with illumination Between increase and increase, in ultra-blue-light and red blue 4:Content of nitrite continues to decline under 1 group of light combination process, in continuous processing 24 Occur minimum point after hour, continue illumination content of nitrite and raise on the contrary.Ultra-blue-light and red blue 4:It is sub- under 1 group of light combination process The minimum of nitrate content is respectively 0.07 μ g/g, 0.02 μ g/g, is below monolithic white light, the minimum of monochromatic feux rouges treatment (respectively 0.09 μ g/g, 0.12 μ g/g), and red blue 4:Content of nitrite is significantly lower than the same period all the time under 1 group of light combination process Other three groups of LED light source treatment, the content of nitrite in continuous processing 10h~48h is not higher than 0.05 μ g/g, and minimum exists The content of nitrite (0.16 μ g/g) of 0.02 μ g/g or so, substantially less than before processing.
3.3 different light sources process the influence to Chinese cabbage quality in 24 hours
1 is shown in Table, by after continuous 24h treatment, soluble sugar and soluble protein content are red blue 4:Under 1 combination light most Height, is significantly higher than other three treatment;Content of glutamic acid highest under blue light, content of glutamic acid is significantly higher than at white light and feux rouges Reason, with red blue 4:1 group of light combination process does not have difference;Glutamine content highest under white light, but difference between four treatment It is not notable.
The different LED lights of table 1 process the 24 small C N metabolism product assays of pakchoi at present
3.4 different light sources process the influence to Chinese cabbage chlorophyll content in 24 hours
See Fig. 4, compared with control treatment, the lower chlorophyll content of feux rouges treatment is minimum, red blue 4:1 group of light combination process inferior lobe is green Cellulose content highest.And red blue 4:Chlorophyll a/b ratio highest under 1 combination light, illustrates that blade resistance is stronger.
3.5 different light sources process 24 hours and the influence for building enzymatic activity are closed to Chinese cabbage nitrogen metabolism
2 are shown in Table, result of the test shows nitrate reductase activity, nitrite reductase activity and glutamate synthetase Red blue 4:Highest under 1 combination light, is significantly higher than other three treatment.Next to that blue light is processed, the lower three kinds of enzymatic activitys of white light are most It is low.
The different LED lights of table 2 process nitrogen metabolism enzyme activity in the 24 small blades of pakchoi at present
Composite score finds that Chinese cabbage carries out soilless culture, 4 lower matter for the treatment of in Huo Gelande nutrient solutions Amount difference is smaller, red blue 4:After continuously being placed 24 hours under 1 combination light or ultra-blue-light treatment, the development of Chinese cabbage is good Good, plant strain growth is healthy and strong, and the nutrition content such as soluble sugar, soluble protein is higher;And energy consumption is relatively low, beneficial to saving into This;NO3-N and NO2-N content is relatively low.Especially with red blue 4:1 combination light continuous processing 24h, nitrate and nitrous acid Salt content is substantially less than other three kinds of light source treatment, nitrate reductase activity, nitrite reductase activity and glutamic acid Synzyme is significantly higher than other three treatment, therefore, can be carried out within 24 hours quickly using blue light Continuous irradiation in facility cultivation Effective treatment, the purpose of Chinese cabbage security quality is improved to reach.

Claims (10)

1. a kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content, it is characterised in that bag Include following steps:
(1), the preparation of seedling:Chinese cabbage seed soaks 6~10 hours in being first put into clear water, places into the sea of clear water immersion Nursery in continuous seedling growing block, after seedling two panels true leaf is fully deployed, water-filling is entered in the hydroponic box that seedling immigration is filled nutrient solution Training;
(2), LED light source treatment:When Chinese cabbage grows to 8~16 true leaves, 10 are processed under continuous illumination under LED light source ~48 hours, 22~25 DEG C of day temperature, 18~20 DEG C of nocturnal temperature, light intensity was set to 100~150 μm of olm-2·s-1, phase To humid control 60%~80%.
2. utilization LED light source according to claim 1 reduces the cultural method of Chinese cabbage nitrate content, its feature It is that rearing condition is in step (1):Under the conditions of natural lighting, 20 ± 2 DEG C of temperature, photoperiod 12hd-1, relative humidity control System is 80%~90%;Water planting condition is:Under the conditions of natural lighting, 20 ± 2 DEG C of temperature, photoperiod 12hd-1, relative humidity control System is 80%~90%.
3. utilization LED light source according to claim 1 reduces the cultural method of Chinese cabbage nitrate content, its feature It is that described nutrient solution is Huo Gelande nutrient solutions in step (1).
4. utilization LED light source according to claim 1 reduces the cultural method of Chinese cabbage nitrate content, its feature It is in step (2), when Chinese cabbage grows to 10 true leaves, to be processed under continuous illumination under LED light source.
5. utilization LED light source according to claim 1 reduces the cultural method of Chinese cabbage nitrate content, its feature It is that described LED light source is white light, monochromatic feux rouges, ultra-blue-light or red blue 4 in step (2):1 combination light.
6. utilization LED light source according to claim 5 reduces the cultural method of Chinese cabbage nitrate content, its feature It is that described LED light source is ultra-blue-light or red blue 4:1 combination light.
7. utilization LED light source according to claim 6 reduces the cultural method of Chinese cabbage nitrate content, its feature It is that described LED light source is red blue 4:1 combination light.
8. utilization LED light source according to claim 1 reduces the cultural method of Chinese cabbage nitrate content, its feature It is to be processed 10~24 hours under continuous illumination under LED light source.
9. utilization LED light source according to claim 8 reduces the cultural method of Chinese cabbage nitrate content, its feature It is to be processed 24 hours under continuous illumination under LED light source.
10. utilization LED light source according to claim 1 reduces the cultural method of Chinese cabbage nitrate content, and it is special Levy is that described LED light source is by LED light lamp and drives adjuster to control light intensity and electric current.
CN201611228045.XA 2016-12-27 2016-12-27 A kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content CN106718755A (en)

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