CN105815197A - Method for improving quality of hydroponic vegetables by use of blue light LED for supplementing light - Google Patents
Method for improving quality of hydroponic vegetables by use of blue light LED for supplementing light Download PDFInfo
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- CN105815197A CN105815197A CN201610287046.5A CN201610287046A CN105815197A CN 105815197 A CN105815197 A CN 105815197A CN 201610287046 A CN201610287046 A CN 201610287046A CN 105815197 A CN105815197 A CN 105815197A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a method for improving the quality of hydroponic vegetables by use of a blue light LED for supplementing light. In 7-10 days before harvesting hydroponic vegetables, performing continuous blue light LED supplementary lighting to the hydroponic vegetables for 10-12 hours each day, so as to enhance the accumulation of soluble sugar, vitamin C, total phenols, flavonoids, anthocyanin, and thioglycoside nutrients in the hydroponic vegetables, improve the FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant capacity and reduce the nitrate content, wherein blue light has wavelength of 450-460nm and illumination intensity of 50-150 micro mol.m<2>.s<1>. The method is suitable for hydroponic vegetables, can effectively improve the quality of the vegetables by supplementing light with the blue light LED simply before harvesting, can reduce the nitrate content, is simple, and has low cost and reliable result.
Description
Technical field
The present invention relates to vegetable aquaculture quality regulation and control and the field that develops skill, in particular for gathering before
Quality of vegetable carry out the method that regulates and controls, utilize blue-ray LED light filling to improve water planting more particularly to one
The method of quality of vegetable.
Background technology
Vegetable is one of food indispensable in people's routine health diet, can be that the mankind provide various
Protein, carbohydrate, mineral, vitamin and various useful bioactive substance, to people
The health of body plays very important effect.Therefore the soluble protein in vegetable, soluble sugar, dimension
The nutritious substances accumulations such as raw element C, total phenols, flavonoid, anthocyanin, sulfur glycosides seem and are even more important.It addition,
Vegetable easily accumulates a kind of material nitrate that health exists great threat.In order to obtain
The vegetable of high-quality, how people's one, the most how at culturing vegetable, improves culturing vegetable
The content of the nutrient substance such as soluble protein, soluble sugar, vitamin C also reduces nitrate therein
The accumulation of content.
Quality of vegetable regulation and control are divided into the regulation and control of soil vegetable and vegetable aquaculture to regulate and control, wherein, and vegetable aquaculture,
Refer to major part root growth in nutritional solution liquid layer, only by nutritional solution for its provide moisture, nutrient,
Oxygen be different from the vegetable carrying out cultivating under conventional soil planting form.Soilless culture (water planting) is no
It is only relevant with soil, root system one-side technical measures again, but has been formed as a kind of existing
Technically highly dense is supporting, reach scientific optimization in management, produce and realize high yield, high-quality, low
Consumption, the agriculture production technology new system efficiently required, have various advantages, therefore, and soilless culture
(water planting) be realize vegetable by tradition flower garden produce to batch production, scale, intensive conversion new
Type planting type.
It is whole that the regulation and control of quality of vegetable include that long period regulation and short-term regulation and control, long period regulation refer to vegetable
Individual cultivating process is implemented various environment and nutrition regulation, including luminosity, moisture and fertilising etc. mode with
Realize the more preferable growth promoter of vegetable；And short-term regulation and control refer to a certain stage of vegetable growth or certain for the moment
Phase regulates and controls.In general, long period regulation needs substantial amounts of manpower and materials, regulation and control cost high and
Because fertilising etc. uses chemical substance to cause degradation defect under quality of vegetable.And short-term regulation and control instant effect, one-tenth
This is low and quality of vegetable raising is played material impact.It has been reported that quality of vegetable short-term regulation and control arrange
Execute and include that forms of nitrogen, Different Nitrogen Concentration adjust, add to infiltrate and leave son etc. under order.Wherein, in typical report
Display before gathering by nitrogen-free nutritional solution replace cultivating nutrient solution regulate and control quality of vegetable method or
Directly remove the nitrogen quantity in nutritional solution or add to infiltrate on the basis of removing nitrate nitrogen and leave son under order to reduce
The accumulation of nitrate, its research shows, can significantly reduce containing of Herba Spinaciae nitrate after processing in a few days
Measure and improve the content of vitamin.But, the method there is also following problem: (1) is owing to eliminating
Or decreasing nitrogenous fertilizer usage amount so that the yield of vegetable has declined, prolongation over time, this shadow
Ring and become apparent from；(2) above-mentioned measure reduces the content of other nutrient substance, the most after treatment
The content of the saccharide in vegetable significantly reduces；(3) above-mentioned regulation measure needed in the vegetable growth later stage
Change nutritional solution, considerably increase management difficulty and production cost.
The raising of quality of vegetable is mainly realized by the optimum management of nutritional solution.Either soil
Cultivation or soilless culture, just affect one of most important envirment factor of growth and development of plants, and it is right
The processes such as the photosynthesis of plant, nitrate metabolism suffer from significant impact, but, pass through light filling
Promoted the technology of quality of vegetable aquaculture before especially gathering there is not been reported by blue light light filling.
Summary of the invention
In order to overcome the defect of above quality of vegetable regulation and control method, the applicant's one utilizes blue-ray LED
Light filling improves the method for vegetable aquaculture quality, and it is improved after being regulated and controled by short-term blue light light filling before gathering
The soluble sugar of vegetable aquaculture, vitamin C, total phenols, flavonoid, anthocyanin, sulfur glycosides nutrient substance,
Improving FRAP, DPPH oxidation resistance simultaneously and reduce nitrate content, the method is easy to operate, becomes
This is low, and effect significantly and not by natural lighting condition is affected.
The purpose of the present invention is achieved through the following technical solutions, and one utilizes blue-ray LED light filling to improve water
The method of training quality of vegetable, the method is before vegetable aquaculture is gathered 7-10 days, imposes vegetable aquaculture
7-10 days 10-12 hour every days, contineous blue light LED light filling, increased its internal soluble sugar, dimension life
Element C, total phenols, flavonoid, anthocyanin, sulfur glycosides nutritious substances accumulation, improve FRAP, DPPH simultaneously
Oxidation resistance also reduces nitrate content.
Above-mentioned blue light wavelength is 450-460nm.
Above-mentioned intensity of illumination is 50～150 μm ol m-2·s-1。
The above-mentioned contineous blue light LED light filling time is that at 6 in the morning to 6 pm or 8 a.m. are to afternoon 6
Point.
Said method is applicable to the vegetable of water planting.
The invention has the beneficial effects as follows: (1) technical scheme utilizes before vegetable aquaculture is gathered
Use blue-ray LED light filling, effectively promote its nutritional quality and health care composition, i.e. increase water planting
Soluble sugar in vegetable body, vitamin C, total phenols, flavonoid, anthocyanin, sulfur glycosides nutrient substance amass
Tired, improve FRAP, DPPH oxidation resistance simultaneously and reduce nitrate content, overcoming in production practices
The chemical substance regulation and control vegetable aquaculture qualities such as nutritional solution are often used to easily cause the shortcomings such as Vegetable pollution；(2)
Utilizing blue-ray LED light filling regulation and control vegetable aquaculture quality is the effective and simple and easy to do new technique of an environmental protection,
Advantage highlights；(3) operational approach of the present invention is simple, low cost, reliable results.
Detailed description of the invention
Embodiment 1
1.1 test materials and test method
Test vegetable material is greenery Plantula Brassicae chinensis.Greenery Plantula Brassicae chinensis nursery and early stage are cultivated all in south China
College of Horticulture of agriculture university is carried out, and is seeded in nursery sponge on April 5th, 2015, and April 25 transplanted
To hydroponic device, before greenery Plantula Brassicae chinensis is gathered 7 days, i.e. May 13 selects the greenery of neat and consistent
Plantula Brassicae chinensis, is divided into the greenery Plantula Brassicae chinensis of neat and consistent four groups of process, often organizes 40 strains, one of which
As control treatment, other three groups keep greenery little Bai by the density and height adjusting LED respectively
Intensity of illumination at dish height is respectively 50 μm ol m-2·s-1、100μmol·m-2·s-1With
150μmol·m-2·s-1, every morning 6 to 6 pm water planting greenery to three process groups respectively
Plantula Brassicae chinensis imposes 12 hours blue-ray LED light fillings, and wherein, blue light wavelength is 450nm, at test plant
Keeping temperature is about 25 DEG C.Greenery Plantula Brassicae chinensis early stage is cultivated and is all adopted during the test of blue-ray LED light filling
Using identical culture fluid, its nutritional solution composition is as follows: Hoagland standard liquid nutrient 1/2 dosage.
Through the greenery Plantula Brassicae chinensis of three process groups is carried out 7 days by a definite date, the blue-ray LED of 12 hours every days
After light filling processes, from matched group and three process groups, take 15 strain greenery Plantula Brassicae chinensis respectively at random, after sampling
Test immediately, test event include soluble protein, soluble sugar, nitrate, vitamin C,
Free amino acid, total phenols, flavonoid, anthocyanin and the content of total sulfur glycosides, all of test event is equal
Use blade testing.
The mensuration of nitrate and soluble sugar is respectively adopted salicylic acid spectrophotometric colo method and phenol
Method.2,6-Dichlorophenol indophenol solution titration vitamin C lixiviating solution is used to try to achieve Vitamin C content.
1.2 results and analysis
As shown in Table 1, compared with comparison, when blue light light filling intensity is 50 μm ol m-2·s-1Time, green
The content of soluble protein, soluble sugar and free amino acid in leaf Plantula Brassicae chinensis blade is the most significantly
Difference, along with the increase of blue light light filling intensity, soluble protein, soluble sugar and free amino acid
Content all significantly reduces, as it can be seen from table 1 the content of the soluble sugar in blade is from 2.39mg/g
Drop to 1.29mg/g, improve 1.8 times；The content of the soluble protein in blade is from 6.33mg/g
Drop to after 6.08mg/g in stable tendency, if it is expected that continue to strengthen the intensity of blue light light filling,
The content of the soluble protein in greenery Plantula Brassicae chinensis blade can be stablized relatively；In blue light light filling intensity it is
150μmol·m-2·s-1Time, the content of free amino acid drops to 24.69 μ from 31.02 μ g/g
g/g.It is 50 μm ol m in blue light light filling intensity-2·s-1Time, nitrate and ascorbic content phase
To constant, along with the enhancing of blue light light filling intensity, ascorbic content dramatically increases, and in illumination
Intensity is 150 μm ol m-2·s-1Time, bring up to 2.04mg/g from 0.45mg/g, improve 4.5
Times；And along with the enhancing of blue light light filling intensity, the accumulation inhibitory action of nitrate is got over by greenery Plantula Brassicae chinensis
Substantially.If from the above it is expected that continue to improve the intensity of blue light light filling, greenery Plantula Brassicae chinensis leaf
Continuation is improved by the content of soluble sugar, vitamin C and free amino acid in sheet.
As shown in Table 2, compared with comparison, along with the increase of blue light light filling intensity, greenery Plantula Brassicae chinensis leaf
The content of total phenols, flavonoid, anthocyanin and total sulfur glycosides in sheet dramatically increases, wherein, and containing of total phenols
Amount increases to 24.34U/g from 20.43U/g；The content of flavonoid increases to 74.7 from 48.31U/g
U/g, adds 26.39U/g, and the content of anthocyanin increases to 2.92U/g from 0.96U/g, improves
3 times；The content of total sulfur glycosides increases to 3.06U/g from 2.78U/g.Meanwhile, along with blue-ray LED
The increase of light filling intensity, oxidation resistance FRAP, DPPH dramatically increase.
Table 1 is after the blue-ray LED light filling of continuous 7 day 12 hours every day in greenery Plantula Brassicae chinensis blade
The change of soluble protein, soluble sugar, nitrate, vitamin C and free aminoacid content.
Table 2 is total in greenery Plantula Brassicae chinensis blade after the blue-ray LED light filling of continuous 7 day 12 hours every day
Phenol, flavonoid, anthocyanin and the change of total sulphur resources.
Embodiment 2
2.1 test materials and test method
Test vegetable material is red autumnal leaves Plantula Brassicae chinensis.Red autumnal leaves Plantula Brassicae chinensis nursery and early stage are cultivated all in south China
College of Horticulture of agriculture university is carried out, and is seeded in nursery sponge on May 5th, 2015, and May 25 transplanted
To hydroponic device, before red autumnal leaves Plantula Brassicae chinensis is gathered 10 days, i.e. June 10 selects the red of neat and consistent
Leaf Plantula Brassicae chinensis, is divided into the red autumnal leaves Plantula Brassicae chinensis of neat and consistent four groups of process, often organizes 40 strains, Qi Zhongyi
Group is as control treatment, and other three groups keep red autumnal leaves little by the density and height adjusting LED respectively
The intensity of illumination that Chinese cabbage is highly located is respectively 50 μm ol m-2·s-1、100μmol·m-2·s-1With
150μmol·m-2·s-1, every morning 8 to 6 pm water planting red autumnal leaves to three process groups respectively
Plantula Brassicae chinensis imposes 10 hours blue-ray LED light fillings, and wherein, blue light wavelength is 460nm, at test plant
Keeping temperature is about 26 DEG C.Red autumnal leaves Plantula Brassicae chinensis early stage is cultivated and is all adopted during the test of blue-ray LED light filling
Using identical culture fluid, its nutritional solution composition is as follows: Hoagland standard liquid nutrient 1/2 dosage.
Through the red autumnal leaves Plantula Brassicae chinensis of three process groups is carried out 7 days by a definite date, the blue-ray LED of 10 hours every days
After light filling processes, from matched group and three process groups, take 15 strain red autumnal leaves Plantula Brassicae chinensis respectively at random, after sampling
Test immediately, test event include soluble protein, soluble sugar, nitrate, vitamin C,
Free amino acid, total phenols, flavonoid, anthocyanin and the content of total sulfur glycosides, all of test event is equal
Use blade testing.
The mensuration of nitrate and soluble sugar is respectively adopted salicylic acid spectrophotometric colo method and phenol
Method.2,6-Dichlorophenol indophenol solution titration vitamin C lixiviating solution is used to try to achieve Vitamin C content.
2.2 results and analysis
As shown in Table 3, compared with comparison, when blue light light filling intensity is 50 μm ol m-2·s-1Time, red
The content of soluble protein, soluble sugar and free amino acid in leaf Plantula Brassicae chinensis blade is the most significantly
Difference, along with the increase of blue light light filling intensity, soluble protein, soluble sugar and free amino acid
Content all significantly reduces, from table 3 it can be seen that the content of the soluble sugar in blade is from 2.05mg/g
Drop to 0.62mg/g, improve 3.3 times；The content of the soluble protein in blade is stable tendency,
If it is expected that continuing to strengthen the intensity of blue light light filling, the soluble protein in red autumnal leaves Plantula Brassicae chinensis blade
Content can relatively stablize；It is 150 μm ol m in blue light light filling intensity-2·s-1Time, free amino acid
Content drop to 28.67 μ g/g from 37.16 μ g/g.In blue light light filling intensity it is
50μmol·m-2·s-1Time, nitrate and ascorbic content relative constancy, along with blue light light filling is strong
The enhancing of degree, ascorbic content dramatically increases, and is 150 μm ol m in intensity of illumination-2·s-1
Time, bring up to 2.56mg/g from 0.55mg/g, improve 4.6 times；And along with blue light light filling intensity
Strengthening, red autumnal leaves Plantula Brassicae chinensis is the most obvious to the accumulation inhibitory action of nitrate.From the above it is expected that
If continuing to improve the intensity of blue light light filling, soluble sugar in red autumnal leaves Plantula Brassicae chinensis blade, vitamin C and
Continuation is improved by the content of free amino acid.
As shown in Table 4, compared with comparison, along with the increase of blue light light filling intensity, red autumnal leaves Plantula Brassicae chinensis leaf
The content of total phenols, flavonoid, anthocyanin and total sulfur glycosides in sheet dramatically increases, wherein, and containing of total phenols
Amount increases to 34.17U/g from 27.10U/g；The content of flavonoid increases to 103.59 from 80.13U/g
U/g, adds 23.46U/g, and the content of anthocyanin increases to 32.57U/g from 18.86U/g, increases
Add 13.71U/g；The content of total sulfur glycosides increases to 5.17U/g from 2.59U/g.Meanwhile, along with
The increase of blue-ray LED light filling intensity, oxidation resistance FRAP, DPPH dramatically increase.
Table 3 is greenery Plantula Brassicae chinensis blade after the blue-ray LED light filling of continuous 10 day 10 hours every day
The change of middle soluble protein, soluble sugar, nitrate, vitamin C and free aminoacid content.
Table 4 is after the blue-ray LED light filling of continuous 10 day 10 hours every day in red autumnal leaves Plantula Brassicae chinensis blade
Total phenols, flavonoid, anthocyanin and the change of total sulphur resources.
Person of ordinary skill in the field is in the feelings of the broader invention concept without departing from above-described embodiment
Under condition, above-described embodiment can be changed, however, it is understood that the invention is not restricted to disclosed
Specific embodiment, but be intended in the spirit and scope of the present invention being defined by the claims
Amendment.
Claims (5)
1. one kind utilizes the method that blue-ray LED light filling improves vegetable aquaculture quality, it is characterised in that
Vegetable aquaculture gather before 7-10 days, vegetable aquaculture is imposed 7-10 days 10-12 hour every days continuously blue
Light LED light filling, increase its internal soluble sugar, vitamin C, total phenols, flavonoid, anthocyanin,
Sulfur glycosides nutritious substances accumulation, improves FRAP, DPPH oxidation resistance simultaneously and reduces nitrate content.
A kind of side utilizing blue-ray LED light filling to improve vegetable aquaculture quality
Method, it is characterised in that blue light wavelength is 450-460nm.
A kind of side utilizing blue-ray LED light filling to improve vegetable aquaculture quality
Method, it is characterised in that intensity of illumination is 50～150 μm ol m-2·s-1。
A kind of side utilizing blue-ray LED light filling to improve vegetable aquaculture quality
Method, it is characterised in that the contineous blue light LED light filling time is at 6 in the morning to 6 pm or the morning 8
Point is to 6 pm.
A kind of side utilizing blue-ray LED light filling to improve vegetable aquaculture quality
Method, it is characterised in that the method is applicable to the vegetable of water planting.
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| CN106258528A (en) * | 2016-08-09 | 2017-01-04 | 华南农业大学 | A kind of cultural method improving lemon mint volatile oil content |
| CN106718755A (en) * | 2016-12-27 | 2017-05-31 | 江苏省农业科学院 | A kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content |
| CN107027611A (en) * | 2017-06-19 | 2017-08-11 | 惠州学院 | It is a kind of to improve the roxburgh anoectochilus terminal bud tissue-cultured seedling speed of growth and the method for active component content |
| CN107371735A (en) * | 2017-08-23 | 2017-11-24 | 华南农业大学 | It is a kind of to couple the method for carrying out vegetables production with nutrient solution using LED illumination, time |
| CN107771554A (en) * | 2017-06-27 | 2018-03-09 | 华南农业大学 | A kind of method for improving seedling vegetable matter using blue violet light LED interruption light fillings |
| CN111512819A (en) * | 2020-05-06 | 2020-08-11 | 中国农业科学院都市农业研究所 | Method for increasing content of nutrient components of red beet by blue-violet light coupling treatment |
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| CN113728904A (en) * | 2021-07-23 | 2021-12-03 | 中国农业科学院都市农业研究所 | Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258528A (en) * | 2016-08-09 | 2017-01-04 | 华南农业大学 | A kind of cultural method improving lemon mint volatile oil content |
| CN106258528B (en) * | 2016-08-09 | 2021-12-28 | 华南农业大学 | Cultivation method for increasing content of lemon balm volatile oil |
| CN106718755A (en) * | 2016-12-27 | 2017-05-31 | 江苏省农业科学院 | A kind of utilization LED light source quickly effectively reduces the cultural method of Chinese cabbage nitrate content |
| CN107027611A (en) * | 2017-06-19 | 2017-08-11 | 惠州学院 | It is a kind of to improve the roxburgh anoectochilus terminal bud tissue-cultured seedling speed of growth and the method for active component content |
| CN107771554A (en) * | 2017-06-27 | 2018-03-09 | 华南农业大学 | A kind of method for improving seedling vegetable matter using blue violet light LED interruption light fillings |
| CN107371735A (en) * | 2017-08-23 | 2017-11-24 | 华南农业大学 | It is a kind of to couple the method for carrying out vegetables production with nutrient solution using LED illumination, time |
| CN111512819A (en) * | 2020-05-06 | 2020-08-11 | 中国农业科学院都市农业研究所 | Method for increasing content of nutrient components of red beet by blue-violet light coupling treatment |
| CN113141905A (en) * | 2020-12-29 | 2021-07-23 | 中国农业科学院都市农业研究所 | Method for improving quality of facility Chinese cabbage before harvesting |
| CN113229095A (en) * | 2021-05-17 | 2021-08-10 | 上海交通大学 | Method for improving ornamental value of lotus flower |
| CN113728904A (en) * | 2021-07-23 | 2021-12-03 | 中国农业科学院都市农业研究所 | Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling |
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