CN104557200A - Microelement culture solution and method for increasing microelements of pea sprouts with same - Google Patents

Microelement culture solution and method for increasing microelements of pea sprouts with same Download PDF

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Publication number
CN104557200A
CN104557200A CN201410843321.8A CN201410843321A CN104557200A CN 104557200 A CN104557200 A CN 104557200A CN 201410843321 A CN201410843321 A CN 201410843321A CN 104557200 A CN104557200 A CN 104557200A
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China
Prior art keywords
pea
nutrient solution
trace element
sprouts
pea sprouts
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CN201410843321.8A
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Chinese (zh)
Inventor
朱世东
刘海燕
袁凌云
汪承刚
单国雷
赵冠艳
何婷婷
吴春祥
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Anhui Agricultural University AHAU
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Anhui Agricultural University AHAU
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Priority to CN201410843321.8A priority Critical patent/CN104557200A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • C05D9/02Other inorganic fertilisers containing trace elements
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Soil Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a microelement culture solution and a method for increasing microelements of pea sprouts with the same. The microelement culture solution is a solution containing one or more of 0.3-0.6 mg/L of zinc sulfate, 300-500 mg/L of organic selenium and 200-400 mg/L of germanium dioxide. The method for increasing the microelements of the pea sprouts comprises steps as follows: firstly, pea seeds are soaked in the microelement culture solution and then cultured, and the microelement culture solution is sprayed once every 12-24 h during culture. Compared with the prior art, the microelement culture solution and the method have the advantages as follows: the microelements of the cultured pea sprouts are high in enrichment degree, the microelement culture solution can also promote growth of the pea sprouts, production is not limited by seasons, the production cycle is short, the cost is low, the benefit is high, and the pea sprouts are natural and pollution-free.

Description

A kind of method of micro-nutrient solution and raising Pea Sprouts trace element thereof
Technical field
What the present invention relates to is a kind of technical field of preparation of functional foodstuff, the in particular a kind of method of micro-nutrient solution and raising Pea Sprouts trace element thereof.
Background technology
Existing trace element enrichment concentrates in wheat, tealeaves, glossy ganoderma or other edible mushroomss mostly, trace element in bud seedling vegetable also has research and report, but be mostly to study it to trace element enrichment, a kind of method of raising micronutrient levels of optimum can not be considered.Chinese patent literature ZL201010203459.3 discloses one " production method of the bud seedling vegetable of one or more trace elements of enrichment ", the salt compounds of trace element is dissolved in the nutrient solution of obtained bud seedling vegetable in a certain amount of clear water by the method, but the method does not consider a kind of condition allowing bud seedling vegetable grow of optimum, simultaneously, use Sodium Selenite as selenium enrichment of element nutrient solution in this patent, and the inorganic reagent of selenium all has severe toxicity, be therefore used in nutrient solution and there is certain danger.Go out through experimental study, the growth of micro-nutrient solution to seed and bud seedling vegetable of different concns is influential, certain density trace element can affect the growth cycle of bud seedling vegetable, output and physical signs, may promote the growth that also may suppress bud seedling vegetable.Further, too high trace element enrichment, has exceeded the scope that human body can bear, will have too much of a good thing, and can produce harm on the contrary, run counter to the original intention of functional foodstuff to human body.Therefore, obtain a kind of nutrient solution concentration and formula of optimum, to the enrichment of the trace element of bud seedling vegetable, the growth of bud seedling vegetable self and produce the bud seedling vegetable that the most applicable people eat and have important meaning.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of method of micro-nutrient solution and raising Pea Sprouts trace element thereof, to provide a kind of optimum nutrient solution prescription and content, while ensureing Pea Sprouts own growth, improve the content of its trace element.
The present invention is achieved by the following technical solutions:
The invention provides a kind of Pea Sprouts trace element nutrient solution, described micro-nutrient solution is containing one or more the solution in the germanium dioxide of the zinc sulfate of 0.3 ~ 0.6mg/L, the organoselenium of 300 ~ 500mg/L and 200 ~ 400mg/L.
Further preferably, described micro-nutrient solution is one or more the solution in zinc sulfate, the organoselenium of 400mg/L and the germanium dioxide of 300mg/L containing 0.5mg/L.
Present invention also offers a kind of method utilizing above-mentioned Pea Sprouts trace element nutrient solution to improve Pea Sprouts trace element, step comprises: cultivate after first being soaked with described micro-nutrient solution by pea seed again, between incubation period, spray once every the described micro-nutrient solution of 12 ~ 24h.
The step of described immersion is: first sterilized by pea seed, and then every 100g pea seed micro-nutrient solution of 100 ~ 120mL soaks 15 ~ 20 hours.
The step of described cultivation comprises:
(1) the lucifuge vernalization phase: the pea seed after soaking is placed in the seedling pan that bottom is covered with thieving paper, it covers the gauze of two-layer sterilizing, to ensure emerge neat and keep moistening, be then placed in the constant incubator lucifuge vernalization of 20 ~ 28 DEG C, obtain pea sprouting seedling;
(2) in lucifuge vegetative period: when the pea sprouting seedling of described step (1) grows to 2 ~ 4cm, take gauze off and continue free growth in the constant incubator of 20 ~ 28 DEG C by it, and remove the abnormal pea sprouting seedling of growth at any time;
(3) see light incubation period: treat that the pea sprouting seedling of described step (2) grows to 8 ~ 10cm, start to see that light is cultivated, when pea sprouting seedling grows to 12 ~ 15cm, described in namely obtaining, be rich in the Pea Sprouts of trace element.
During the lucifuge vernalization of described step (1), spray described micro-nutrient solution every 24h.
The lucifuge growing period of described step (2), sprays described micro-nutrient solution every 12h.
Described step (3) see between light incubation period, spray described micro-nutrient solution every 12h.
The present invention has the following advantages compared to existing technology:
A, the Pea Sprouts utilizing this nutrient solution to cultivate acquisition are rich in multiple nutrients, and the trace element of enrichment is all organic, safety and Health, when directly eating as vegetables for people, can reach the same effect treated of medicine food;
B, micro-nutrient solution of the present invention cultivate the Pea Sprouts obtained, and not only micronutrient levels is high, and its amino acid whose content also has overall rising, the eight large aminoacids contents that particularly human body is necessary, increasing to some extent in varying degrees all respectively, in germanium process be such as, TYR adds 111.69%, arginine content adds 85.54%, methionine contents adds 58.43%, Glycine Levels adds 56.02%, except lysine content have dropped 9.19%, the amino acid classes of other composition human body has the growth of more than 20%.
C, the present invention cultivate the total economic value capability improving of the Pea Sprouts of acquisition, control group be 0.03146 unit/μ g albumen, and under the process of selenium element, total economic value reaches 0.1762 unit/μ g albumen; Harmful free radicals in the effective purged body of polyphenoils mass-energy, prevents lipid peroxidation, prevents the oxidative damage of radical pair biomacromolecule, ensures the normal of cellular structures and functions, thus reduces the generation of disease.Rational dietary nutrition, takes in rational anti-oxidant nutritive substance and also can keep normal anti-oxidant function, delaying senility.
The method of the Pea Sprouts of D, cultivation rich trace elements of the present invention can factorial praluction, produce not by seasonal restriction, (8 ~ 10 days) with short production cycle, cost is low, high efficiency, natural nuisance-free, its technological process of production to product and environment all pollution-free, and more can play in the flourishing city of an inch of land is an inch of gold soil plantation be worth.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
Present embodiments provide a kind of method improving Pea Sprouts trace element, comprise the following steps:
(1) after being sterilized by pea seed, the micro-nutrient solution of every 100g pea seed 100mL soaks 18 hours, and described micro-nutrient solution is the solution of zinc sulfate of 0.5mg/L, obtains the pea seed after seed soaking.
(2) the pea seed after step (1) being soaked seed is seeded to bottom and is covered with in the seedling pan of thieving paper, it covers the gauze of two-layer sterilizing, to ensure emerge neat and keep moistening, then 28 DEG C are placed in, relative humidity is lucifuge vernalization in the fixed temperature and humidity incubator of 60%, and period sprays the solution of zinc sulfate of 0.5mg/L every 24h.
(3) two days later, pea sprouting seedling grows to 2 ~ 4cm, statistics percentage of germination is 97.2%, take gauze off by its free growth, then, continue to cultivate in the constant incubator of 28 DEG C, period sprays the solution of zinc sulfate of 0.5mg/L every 12h, the amount of spraying is 25ml, and removes the abnormal bud seedling of growth at any time.
(4) when pea sprouting seedling grows to 10 ~ 13cm, be placed in illumination box, at 22 ~ 28 DEG C, light cultivates two days, and period continues every 12h the solution of zinc sulfate spraying 0.5mg/L, when pea sprouting seedling plant height grows to 12 ~ 15cm, obtain rich zinc Pea Sprouts.
Embodiment 2
Present embodiments provide a kind of method improving Pea Sprouts trace element, comprise the following steps:
(1) after being sterilized by pea seed, the micro-nutrient solution of every 100g pea seed 120mL soaks 15 hours, and described micro-nutrient solution is the germanium dioxide solution of 300mg/L, obtains the pea seed after seed soaking.
(2) the pea seed after step (1) being soaked seed is seeded to bottom and is covered with in the seedling pan of thieving paper, it covers the gauze of two-layer sterilizing, to ensure emerge neat and keep moistening, then 25 DEG C are placed in, relative humidity is lucifuge vernalization in the fixed temperature and humidity incubator of 60%, and period sprays the micro-nutrient solution described in step (1) every 24h.
(3) two days later, pea sprouting seedling grows to 2 ~ 4cm, statistics percentage of germination is 98.7%, take gauze off by its free growth, then, continue to cultivate in the constant incubator of 25 DEG C, period sprays the micro-nutrient solution described in step (1) every 12h, and removes the abnormal bud seedling of growth at any time.
(4) when pea sprouting seedling grows to 8 ~ 10cm, start to see that light is cultivated, period continues to spray the micro-nutrient solution described in step (1) every 12h, when pea sprouting seedling plant height grows to 12 ~ 15cm, obtains rich germanium Pea Sprouts.
Be rich in germanium organic matter in rich germanium Pea Sprouts, people can be helped to set up, prevent anaemia, help metabolism etc., organic germanium compounds also has the mechanism of Tumor suppression activity, comprises enhancing body immunizing power, the many aspects such as scavenging free radicals and anti-mutation.
Embodiment 3
Present embodiments provide a kind of method improving Pea Sprouts trace element, comprise the following steps:
(1), after being sterilized by pea seed, the micro-nutrient solution of every 100g pea seed 110mL soaks 20h hour, and described micro-nutrient solution is the organoselenium solution of 400mg/L, obtains the pea seed after seed soaking.
(2) the pea seed after step (1) being soaked seed is seeded to bottom and is covered with in the seedling pan of thieving paper, it covers the gauze of two-layer sterilizing, to ensure emerge neat and keep moistening, then 20 DEG C are placed in, relative humidity is lucifuge vernalization in the fixed temperature and humidity incubator of 60%, and period sprays the micro-nutrient solution described in step (1) every 24h.
(3) two days later, pea sprouting seedling grows to 2 ~ 4cm, statistics percentage of germination is 96.9%, take gauze off by its free growth, then, continue to cultivate in the constant incubator of 20 DEG C, period sprays the micro-nutrient solution described in step (1) every 12h, the amount of spraying is 25ml, and removes the abnormal bud seedling of growth at any time.
(4) when pea sprouting seedling grows to 8 ~ 10cm, start to see that light is cultivated, period continues to spray the micro-nutrient solution described in step (1) every 12h, when pea sprouting seedling plant height grows to 12 ~ 15cm, is described rich selenium Pea Sprouts.
Rich selenium Pea Sprouts can be used as the supplement of selenium element, is applicable to scarce selenium crowd, can be anti-ageing, maintain vigour, and selenium is the important substance of nervous system development, be the important composition material of immunity system synthetic, being supplemented with of appropriate selenium is beneficial to healthy.
Measure the physiology and appearance of Pea Sprouts and the nutritive index of embodiment 1 ~ 3, comprise eight necessary seed amino acid total contents of Zn content, ge content, selenium content, human body, single amino acids content, Vitamin C content,
Protein content, total economic value and Chlorophyll-a Content, and replace micro-nutrient solution process Pea Sprouts with clear water, as the negative control before optimization, concrete outcome is as shown in table 1 below:
Table 1: the physiology and appearance of the Pea Sprouts of embodiment 1 ~ 3 and nutritive index measurement result synopsis
As can be seen from Table 1, no matter be use zinc process, or germanium or selenium process, the content of its corresponding trace element has a larger increase, simultaneously, the content of the indispensable amino acid in the Pea Sprouts after process, vitamins C, protein, total economic value and chlorophyll a all increases by a fairly big margin, and illustrates that method of the present invention can not only improve the content of the trace element of Pea Sprouts, and can promote the growth of Pea Sprouts.
Embodiment 4
Present embodiments provide a kind of method improving Pea Sprouts trace element, wherein, described trace element is the mixing solutions of zinc sulfate, the organoselenium of 500mg/L and the germanium dioxide of 300mg/L containing 0.3mg/L, and other steps, with embodiment 1, obtain the Pea Sprouts of rich zinc selenium germanium.
Embodiment 5
Present embodiments provide a kind of method improving Pea Sprouts trace element, wherein, described trace element is the mixing solutions of the germanium dioxide of organoselenium containing 300mg/L and 200mg/L, and other steps, with embodiment 1, obtain the Pea Sprouts containing selenium germanium.
Embodiment 6
Present embodiments provide a kind of method improving Pea Sprouts trace element, wherein, described trace element is the mixing solutions of the germanium dioxide of zinc sulfate containing 0.5mg/L and 400mg/L, and other steps, with embodiment 1, obtain the Pea Sprouts of rich zinc germanium.

Claims (8)

1. a Pea Sprouts trace element nutrient solution, is characterized in that, described micro-nutrient solution is containing one or more the solution in the germanium dioxide of the zinc sulfate of 0.3 ~ 0.6mg/L, the organoselenium of 300 ~ 500mg/L and 200 ~ 400mg/L.
2. a kind of Pea Sprouts trace element nutrient solution according to claim 1, it is characterized in that, described micro-nutrient solution is one or more the solution in zinc sulfate, the organoselenium of 400mg/L and the germanium dioxide of 300mg/L containing 0.5mg/L.
3. one kind utilizes the method for the trace element as claim 1,2 arbitrary described Pea Sprouts trace element nutrient solution raising Pea Sprouts, step comprises: first soaked by the described micro-nutrient solution of pea seed, and then cultivate, between incubation period, spray once every the described micro-nutrient solution of 12 ~ 24h.
4. a kind of method utilizing Pea Sprouts trace element nutrient solution to improve Pea Sprouts trace element according to claim 3, it is characterized in that, the step of described immersion is: first sterilized by pea seed, and then every 100g pea seed micro-nutrient solution of 100 ~ 120mL soaks 15 ~ 20 hours.
5. a kind of method utilizing Pea Sprouts trace element nutrient solution to improve Pea Sprouts trace element according to claim 3, it is characterized in that, the step of described cultivation comprises:
(1) the lucifuge vernalization phase: the pea seed after soaking is placed in the seedling pan that bottom is covered with thieving paper, it covers the gauze of two-layer sterilizing, guarantee is emerged neat and is kept moistening, is then placed in the constant incubator lucifuge vernalization of 20 ~ 28 DEG C, obtains pea sprouting seedling;
(2) in lucifuge vegetative period: when the pea sprouting seedling of described step (1) grows to 2 ~ 4cm, take gauze off and continue free growth in the constant incubator of 20 ~ 28 DEG C by it, and remove the abnormal pea sprouting seedling of growth at any time;
(3) see light incubation period: treat that the pea sprouting seedling of described step (2) grows to 8 ~ 10cm, start to see that light is cultivated, when pea sprouting seedling grows to 12 ~ 15cm, described in namely obtaining, be rich in the Pea Sprouts of trace element.
6. a kind of method utilizing Pea Sprouts trace element nutrient solution to improve Pea Sprouts trace element according to claim 5, is characterized in that, during the lucifuge vernalization of described step (1), spray described micro-nutrient solution every 24h.
7. a kind of method utilizing Pea Sprouts trace element nutrient solution to improve Pea Sprouts trace element according to claim 5, is characterized in that the lucifuge growing period of described step (2) sprays described micro-nutrient solution every 12h.
8. a kind of method utilizing Pea Sprouts trace element nutrient solution to improve Pea Sprouts trace element according to claim 5, is characterized in that, described step (3) see between light incubation period, spray described micro-nutrient solution every 12h.
CN201410843321.8A 2014-12-30 2014-12-30 Microelement culture solution and method for increasing microelements of pea sprouts with same Pending CN104557200A (en)

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CN107573165A (en) * 2017-09-30 2018-01-12 广西吉朋投资有限公司 A kind of corn seed germinator
CN107602214A (en) * 2017-09-01 2018-01-19 佛山科学技术学院 A kind of nutrient solution for promoting pea root growth
CN108040844A (en) * 2017-11-15 2018-05-18 佛山科学技术学院 A kind of aluminum-resistant poison pea seedling inoculation method
CN108651251A (en) * 2018-04-04 2018-10-16 云南农业职业技术学院 A kind of indoor production technology of edible ornamental shoot vegetable
CN109122258A (en) * 2018-08-10 2019-01-04 安徽省三分地农业发展有限公司 A kind of preparation method of Selenium-enriched radish shoot vegetable
CN109874658A (en) * 2019-03-29 2019-06-14 贵阳学院 A kind of novel selenium-enriched bean sprouts production method
CN111869558A (en) * 2020-07-08 2020-11-03 湖北工程学院 Composite organic selenium nutrient solution, application thereof and cultivation method of sprouting vegetables
CN112056200A (en) * 2020-09-22 2020-12-11 云南泓庆农业科技有限公司 Cultivation method of pea sprout vegetables
CN113475676A (en) * 2021-07-12 2021-10-08 中国农业大学 Cultivation of zinc-rich edible pea seedlings and application of zinc-rich edible pea seedlings in zinc-rich noodles

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107602214A (en) * 2017-09-01 2018-01-19 佛山科学技术学院 A kind of nutrient solution for promoting pea root growth
CN107573165A (en) * 2017-09-30 2018-01-12 广西吉朋投资有限公司 A kind of corn seed germinator
CN108040844A (en) * 2017-11-15 2018-05-18 佛山科学技术学院 A kind of aluminum-resistant poison pea seedling inoculation method
CN108651251A (en) * 2018-04-04 2018-10-16 云南农业职业技术学院 A kind of indoor production technology of edible ornamental shoot vegetable
CN109122258A (en) * 2018-08-10 2019-01-04 安徽省三分地农业发展有限公司 A kind of preparation method of Selenium-enriched radish shoot vegetable
CN109874658A (en) * 2019-03-29 2019-06-14 贵阳学院 A kind of novel selenium-enriched bean sprouts production method
CN111869558A (en) * 2020-07-08 2020-11-03 湖北工程学院 Composite organic selenium nutrient solution, application thereof and cultivation method of sprouting vegetables
CN112056200A (en) * 2020-09-22 2020-12-11 云南泓庆农业科技有限公司 Cultivation method of pea sprout vegetables
CN113475676A (en) * 2021-07-12 2021-10-08 中国农业大学 Cultivation of zinc-rich edible pea seedlings and application of zinc-rich edible pea seedlings in zinc-rich noodles

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Application publication date: 20150429