CN103975722B - The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy - Google Patents
The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy Download PDFInfo
- Publication number
- CN103975722B CN103975722B CN201410168981.0A CN201410168981A CN103975722B CN 103975722 B CN103975722 B CN 103975722B CN 201410168981 A CN201410168981 A CN 201410168981A CN 103975722 B CN103975722 B CN 103975722B
- Authority
- CN
- China
- Prior art keywords
- plastic film
- glutinous rehmannia
- seedling
- test tube
- days
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The present invention relates to outside plant test-tube plantlet and move technical field, the specifically a kind of direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy, the steps include: in test tube, carry out glutinous rehmannia detoxification tissue cultures, grow to the seedling of taking root of 5 ~ 6 leaf one-tenth glutinous rehmannia detoxification, the first tenday period of a month in mid-April to May, the seedling of taking root of virus-free radix rehmanniae is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy of the present invention, eliminate the step that conventional method Middle nutrition alms bowl is transplanted, not only simplify operating process, but also shorten transplanting seedling time, transplant and within 15 days, just grow young leaves, new root afterwards, the 1.85/strain of 30 days mean leaf new increments, key substantially increases survival rate, survival rate, to more than 95%, saves transplanting cost.
Description
Technical field
The present invention relates to outside plant test-tube plantlet and move technical field, specifically a kind of direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy.
Background technology
Glutinous rehmannia is medicinal material conventional on China's traditional Chinese medicine, is subject to the dip-dye of multiple virus and pathogen in planting process, causes the reduction of kind of a sexual involution, output, quality decline.Utilizing stem-apex Meristem culture technology to obtain detoxification plant, is the most economical effective way removing virus disease, and the transplanting survival rate of detoxification test tube plantlet to be virus-elimination seedlings be applied to produces a vital step.
Conventional transplanting method is that glutinous rehmannia detoxification test tube plantlet is opened bottle cap hardening 2 ~ 3 days in laboratory, then be transplanted to Nutrition Soil+vermiculite+peat soil is housed nutritive cube in, nutrient solution also takes moist keeping measures, about 20 days the test-tube seedling transplanting after surviving to field, fly net canopy garden.The glutinous rehmannia detoxic seedling temperature adjustment transplantation technique system that Li Yane etc. set up is transplanted in nutritive cube that sterilization matrix be housed after hardening by test-tube plantlet between cultivation, moisturizing, Low-temperature culture (21-22 DEG C) 3-4 days in illumination box, be warming up to 25-27 DEG C of 4-5 days again, be finally transplanted to land for growing field crops normal growth.Above two kinds method operation is many, survival rate is low, and take a lot of work, time-consuming, cost is high, be not easy to test-tube plantlet scale transplant.
Summary of the invention
The present invention, in order to solve existing glutinous rehmannia detoxicating cuvette method for transplanting Problems existing, provides a kind of direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy.
The present invention is achieved by the following technical solutions: the direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy, the steps include: in test tube, carry out glutinous rehmannia detoxification tissue cultures, grow to the seedling of taking root of 5 ~ 6 leaf one-tenth glutinous rehmannia detoxification, in the first tenday period of a month in mid-April to May, the seedling of taking root of virus-free radix rehmanniae is directly planted into nursery lot, blinding, watering, plastic film shed is built in timely employing, and add a cover sunshade net, carry out covering and to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.
Further, blinding thickness is 2.5 ~ 3.0cm, and the soil moisture content after watering is 13 ~ 14%.Blinding is excessively thin, take root shoot root system and soil Exposure few, easily lodging and outside being easily exposed to, cause seedling dehydration of taking root withered dead; Blinding is blocked up, and rhizome and soil contact portion too much, affect gas permeability, take root shoot root stem easily at this position perish.Soil moisture content is greater than 14%, and humidity is excessive, and seedling transplanting seedling time of taking root is long, the seedling that even can cause taking root because of anoxic, grow germ and perish; Lower than 13%, glutinous rehmannia detoxic seedling can a large amount of dehydration and wilting gradually, and soil moisture is lower, and wilting speed is faster, and transplanting survival rate is lower.
Further, plastic film shed top is far from ground 30 ~ 40cm.Highly determine the size in canopy space, lower than 30cm, seedling of taking root touches ceiling, easily scalds seedling; Higher than 40cm, humidity not easily keeps, and seedling dehydration is too fast and wilt.
Further, the temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 50 ~ 60%.Under above-mentioned environmental condition, slow fast seedling, photosynthesis, nutrient accumulation and the root growth and development of the seedling that is conducive to taking root, improve transplanting survival rate, glutinous rehmannia detoxic seedling robust growth.
Further, cover moisturizing 4th ~ 5 days of sheltering from heat or light, open plastic film shed and carry out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.Reduce the temperature and humidity in canopy gradually according to the upgrowth situation of glutinous rehmannia detoxic seedling, make it final consistent with the epidemic disaster of external environment, object tempers seedling gradually, finally makes it to adapt to grown in field environment.
The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy of the present invention, eliminate the step that conventional method Middle nutrition alms bowl is transplanted, not only simplify operating process, but also shorten transplanting seedling time, transplant and within 15 days, just grow young leaves, new root afterwards, the 1.85/strain of 30 days mean leaf new increments, key substantially increases survival rate, survival rate, to more than 95%, saves transplanting cost.
Embodiment
In order to better the present invention is described, be explained in detail the present invention below by specific embodiment, wherein the tissue cultures of glutinous rehmannia detoxification test tube plantlet is this area routine techniques means.
embodiment 1
The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy utilizes stem-apex Meristem culture technology, by stem of Radix Codonopsis lanceolatae Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0g/L+6-BA(6-benzylaminopurine) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, carries out detoxification test tube plantlet Fast-propagation in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium.Grow to the seedling of taking root of 5 leaf one-tenth glutinous rehmannia detoxification, in the first tenday period of a month in mid-April to May, the seedling of taking root of glutinous rehmannia detoxification is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.
Blinding thickness is 2.7cm, and the soil moisture content after watering is 14%.Plastic film shed top is far from ground 40cm.Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 55%.Covering is sheltered from heat or light moisturizing the 4th day, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into the detoxification of nursery lot glutinous rehmannia is 1500 strains, survives 1443 strains, survival rate 96.20% after 30 days.
embodiment 2
The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy utilizes stem-apex Meristem culture technology, by stem of Radix Codonopsis lanceolatae Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0g/L+6-BA(6-benzylaminopurine) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, carries out detoxification test tube plantlet Fast-propagation in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium.Grow to the seedling of taking root of 6 leaf one-tenth glutinous rehmannia detoxification, in the first tenday period of a month in mid-April to May, the seedling of taking root of glutinous rehmannia detoxification is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.
Blinding thickness is 3.0cm, and the soil moisture content after watering is 13%.Plastic film shed top is far from ground 35cm.Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 60%.Covering is sheltered from heat or light moisturizing the 5th day, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into the detoxification of nursery lot glutinous rehmannia is 650 strains, survives 624 strains, survival rate 96.00% after 30 days.
embodiment 3
The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy utilizes stem-apex Meristem culture technology, by stem of Radix Codonopsis lanceolatae Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0g/L+6-BA(6-benzylaminopurine) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, carries out detoxification test tube plantlet Fast-propagation in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium.Grow to the seedling of taking root of 5 leaf one-tenth glutinous rehmannia detoxification, in the first tenday period of a month in mid-April to May, the seedling of taking root of glutinous rehmannia detoxification is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.
Blinding thickness is 2.5cm, and the soil moisture content after watering is 13.5%.Plastic film shed top is far from ground 30cm.Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 50%.Covering is sheltered from heat or light moisturizing the 4th day, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into the detoxification of nursery lot glutinous rehmannia is 900 strains, survives 861 strains, survival rate 95.67% after 30 days.
Claims (1)
1. the direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy, it is characterized in that, the steps include: in test tube, carry out glutinous rehmannia detoxification tissue cultures, grow to the seedling of taking root of 5 ~ 6 leaf one-tenth virus-free radix rehmanniaes, the first tenday period of a month in mid-April to May, the seedling of taking root of glutinous rehmannia detoxification is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film; Blinding thickness is 2.5 ~ 3.0cm, and the soil moisture content after watering is 13 ~ 14%; Plastic film shed top is far from ground 30 ~ 40cm; Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 50 ~ 60%; Covering is sheltered from heat or light moisturizing 4th ~ 5 days, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168981.0A CN103975722B (en) | 2014-04-25 | 2014-04-25 | The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168981.0A CN103975722B (en) | 2014-04-25 | 2014-04-25 | The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103975722A CN103975722A (en) | 2014-08-13 |
| CN103975722B true CN103975722B (en) | 2016-01-20 |
Family
ID=51268100
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410168981.0A Expired - Fee Related CN103975722B (en) | 2014-04-25 | 2014-04-25 | The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103975722B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104350913B (en) * | 2014-10-23 | 2016-12-07 | 南通市乐悦实业有限公司 | A kind of method for culturing seedlings of decomposite leaf Radix Rehmanniae |
| CN108496600B (en) * | 2018-04-24 | 2020-08-18 | 山西省农业科学院作物科学研究所 | Micro-cuttage rapid propagation method for virus-free rehmannia test-tube plantlets |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100521921C (en) * | 2006-08-21 | 2009-08-05 | 河南省农业科学院植物保护研究所 | Soiless breeding method for micro planting detoxic radix rehmanniae |
| CN102301955A (en) * | 2011-07-19 | 2012-01-04 | 河南师范大学 | Method for breeding rehmannia toxin-free seedling |
-
2014
- 2014-04-25 CN CN201410168981.0A patent/CN103975722B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103975722A (en) | 2014-08-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1324946C (en) | Large-area cultivation of officinal dendrobium | |
| CN101653096A (en) | Method for tissue-culture breeding and wild-simulated cultivation of dendrobium candidum | |
| CN103314738A (en) | Dendrobium huoshanense greenhouse planting method | |
| CN104106468B (en) | The quick breeding method for tissue culture of a kind of radix fici simplicissimae | |
| CN105766385B (en) | A kind of plant protection method improving virus-free basic potato seed yield | |
| CN103168590A (en) | Bletilla striata seed propagating method | |
| CN100388877C (en) | Industrial prodn. of Huan-Cao dendrobe (dendrobium loddigessi Rolfe) technique and culturing method therefor | |
| CN105123529A (en) | Rapid propagation and efficient cultivation method of Bletilla striata | |
| CN104429865A (en) | Sexual propagation method of bletilla striata | |
| CN104956898A (en) | Baphicanthus cusia bremek seedling cultivating method performed through cutting | |
| CN1522561A (en) | Potted loquat and its cultivation method | |
| CN103975723B (en) | Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method | |
| CN104273038B (en) | The tissue culture method of a kind of Mount Huang Luan Shu | |
| CN103168692A (en) | Salix saposhnikovii tissue culture method | |
| CN103975722B (en) | The direct transplantation method of glutinous rehmannia detoxification test tube plantlet net canopy | |
| CN103749135B (en) | The implantation methods that Thesium chinese man plants or strange land is introduced a fine variety | |
| CN103548695B (en) | A kind of meadowrueleaf corydalis root quick breeding method for tissue culture | |
| CN104335788A (en) | Pot-culture method for osmanthus fragrans tree with medical, edible and viewing functions | |
| CN109220799A (en) | A kind of method of artificial pollination tissue culture technology and the cultivation breeding wild HERBA DENDROBII in the Qinling Mountains | |
| CN105210884A (en) | Bletilla seed asepsis sprouting equilibrated Medium | |
| CN105165580B (en) | A kind of cultural method of suitable high latitude dendrobium candidum | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN108391591B (en) | Tissue culture and rapid propagation method for tabebuia flavedo | |
| CN106538331B (en) | A kind of hardening off method of black fruit fructus lycii tissue-cultured seedling | |
| CN105794444A (en) | Germination method for climbing fern spores |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160120 Termination date: 20160425 |