CN103975723B - Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method - Google Patents
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method Download PDFInfo
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- CN103975723B CN103975723B CN201410168985.9A CN201410168985A CN103975723B CN 103975723 B CN103975723 B CN 103975723B CN 201410168985 A CN201410168985 A CN 201410168985A CN 103975723 B CN103975723 B CN 103975723B
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Abstract
The present invention relates to outside plant test-tube plantlet and move technical field, specifically a kind of Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method, the steps include: to carry out Sweetpotato Viruses Elimination tissue cultures in test tube, grow to the seedling of taking root of 5 ~ 6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, plastic film shed is built in timely employing, and add a cover sunshade net, carry out covering and to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.Sweetpotato Viruses Elimination test-tube plantlet net canopy of the present invention is directly transplanted into indigenous method, eliminate the step that conventional method Middle nutrition alms bowl is transplanted, not only simplify operating process, but also shorten transplanting seedling time, transplant and within 15 days, just grow young leaves, new root afterwards, with regard to vigorous growth after 30 days, key substantially increases survival rate, and survival rate is to more than 96%.
Description
Technical field
The present invention relates to outside plant test-tube plantlet and move technical field, specifically a kind of Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method.
Background technology
Sweet potato is important grain, feed, the raw material of industry and novel energy root crop, and be also the bottom line crop ensureing China's grain security, China plants 8,000 ten thousand mu throughout the year, and output accounts for the world and always produces more than 80%.But sweet potato is asexually propagated crop, by the dip-dye of multiple virus and pathogen, cause kind of a sexual involution, output to reduce, quality declines.Current report infects the virus of sweet potato and viroids has more than 20 to plant, especially Virus Diseases of Sweet Potato (SPVD) occurrence tendency is serious, it is reported that SPVD shows disease plant comparatively healthy plant underproduction 79-86%, utilize stem-tip tissue to cultivate and produce virus-free potato seedling, be remove virus, recovery kind merit, improve the unique channel of tuber yield and quality.
Conventional transplanting method is that Sweetpotato Viruses Elimination test-tube plantlet is opened bottle cap hardening 2 ~ 3 days in laboratory, then be transplanted to Nutrition Soil+vermiculite+peat soil is housed nutritive cube in more than 30 days, pouring is transplanted nutrient solution and is taked moist keeping measures, and the detoxification test tube plantlet after surviving is transplanted to the field, garden in fly net canopy again.The method in Sweetpotato Viruses Elimination test-tube seedling transplanting process, plantlet in vitro will through twice fall plant, survival rate is lower, and take a lot of work, time-consuming, cost is high, survival rate is low, be unfavorable for Virus-free Sweetpotato industrialization produce.
Summary of the invention
The present invention, in order to solve existing Sweetpotato Viruses Elimination test-tube seedling transplanting method Problems existing, provides a kind of Sweetpotato Viruses Elimination test-tube plantlet net canopy and is directly transplanted into indigenous method.
The present invention is achieved by the following technical solutions: Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method, the steps include: to carry out Sweetpotato Viruses Elimination tissue cultures in test tube, grow to the seedling of taking root of 5 ~ 6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.
Further, blinding thickness is 2.5 ~ 3.0cm, and the soil moisture content after watering is 13 ~ 14%.Blinding is excessively thin, take root shoot root system and soil Exposure few, easily lodging and outside being easily exposed to, cause seedling dehydration of taking root withered dead; Blinding is blocked up, and rhizome and soil contact portion too much, affect gas permeability, take root shoot root stem easily at this position perish.Soil moisture content is greater than 14%, and humidity is excessive, and seedling transplanting seedling time of taking root is long, the seedling that even can cause taking root because of anoxic, grow germ and perish; Lower than 13%, Sweetpotato Viruses Elimination seedling can a large amount of dehydration and wilting gradually, and soil moisture is lower, and wilting speed is faster, and transplanting survival rate is lower.
Further, plastic film shed top is far from ground 30 ~ 40cm.Highly determine the size in canopy space, lower than 30cm, seedling of taking root touches ceiling, easily scalds seedling; Higher than 40cm, humidity not easily keeps, and seedling dehydration is too fast and wilt.
Further, the temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 50 ~ 60%.Under above-mentioned environmental condition, slow fast seedling, photosynthesis, nutrient accumulation and the root growth and development of the seedling that is conducive to taking root, improve transplanting survival rate, Sweetpotato Viruses Elimination seedling robust growth.
Further, cover moisturizing 3rd ~ 4 days of sheltering from heat or light, open plastic film shed and carry out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.Reduce the temperature and humidity in canopy gradually according to the upgrowth situation of Sweetpotato Viruses Elimination seedling, make it final consistent with the epidemic disaster of external environment, object tempers seedling gradually, finally makes it to adapt to grown in field environment.
Sweetpotato Viruses Elimination test-tube plantlet net canopy of the present invention is directly transplanted into indigenous method, eliminate the step that conventional method Middle nutrition alms bowl is transplanted, not only simplify operating process, but also shorten transplanting seedling time, transplant and within 15 days, just grow young leaves, new root afterwards, with regard to vigorous growth after 30 days, key substantially increases survival rate, and survival rate is to more than 96%.
Embodiment
In order to better the present invention is described, be explained in detail the present invention below by specific embodiment, wherein the tissue cultures of Sweetpotato Viruses Elimination test-tube plantlet is this area routine techniques means.
embodiment 1
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method and utilizes stem-apex Meristem culture technology, by Sweet Potato Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0g/L+6-BA(6-benzylaminopurine) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, form strain after Brazilian morning-glory grafting detection is virus-free, in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium, carry out plantlet bud propagation.Grow to the seedling of taking root of 5 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering and to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film.
Blinding thickness is 2.7cm, and the soil moisture content after watering is 14%.Plastic film shed top is far from ground 40cm.Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 55%.Covering is sheltered from heat or light moisturizing the 3rd day, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into nursery lot Sweetpotato Viruses Elimination is 900 strains, survives 868 strains, survival rate 96.44% after 30 days.
embodiment 2
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method and utilizes stem-apex Meristem culture technology, by Sweet Potato Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0g/L+6-BA(6-benzylaminopurine) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, form strain after Brazilian morning-glory grafting detection is virus-free, in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium, carry out plantlet bud propagation.Grow to the seedling of taking root of 6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering and to shelter from heat or light moisturizing 20 days, remove sunshade net and plastic film.
Blinding thickness is 3.0cm, and the soil moisture content after watering is 13%.Plastic film shed top is far from ground 35cm.Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 60%.Covering is sheltered from heat or light moisturizing the 3rd day, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into nursery lot Sweetpotato Viruses Elimination is 325 strains, survives 312 strains, survival rate 96.00% after 30 days.
embodiment 3
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method and utilizes stem-apex Meristem culture technology, by Sweet Potato Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0g/L+6-BA(6-benzylaminopurine) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, form strain after Brazilian morning-glory grafting detection is virus-free, in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium, carry out plantlet bud propagation.Grow to the seedling of taking root of 5 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt in time and build plastic film shed, and add a cover sunshade net, carry out covering and to shelter from heat or light moisturizing 20 days, remove sunshade net and plastic film.
Blinding thickness is 2.5cm, and the soil moisture content after watering is 13.5%.Plastic film shed top is far from ground 30cm.Temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 50%.Covering is sheltered from heat or light moisturizing the 3rd day, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into nursery lot Sweetpotato Viruses Elimination is 277 strains, survives 266 strains, survival rate 96.03% after 30 days.
Claims (1)
1. Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method, it is characterized in that, the steps include: to carry out Sweetpotato Viruses Elimination tissue cultures in test tube, grow to the seedling of taking root of 5 ~ 6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, plastic film shed is built in timely employing, and add a cover sunshade net, carry out covering and to shelter from heat or light moisturizing 30 days, remove sunshade net and plastic film; Blinding thickness is 2.5 ~ 3.0cm, and the soil moisture content after watering is 13 ~ 14%; Plastic film shed top is far from ground 30 ~ 40cm
;temperature in plastic film shed controls between 17 ~ 32 DEG C, and humidity remains between 70 ~ 85%, and the shading rate of sunshade net is 50 ~ 60%; Covering is sheltered from heat or light moisturizing 3rd ~ 4 days, opens plastic film shed and carries out secondary soil-covering, moisturizing; 7th day, hole drilling ventilation on plastic film shed; 30th day, remove plastic film.
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| CN201410168985.9A CN103975723B (en) | 2014-04-25 | 2014-04-25 | Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method |
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| CN201410168985.9A CN103975723B (en) | 2014-04-25 | 2014-04-25 | Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method |
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| CN103975723B true CN103975723B (en) | 2016-01-20 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106386144B (en) * | 2016-10-09 | 2020-03-31 | 江苏建康职业学院 | Cultivation method of traditional Chinese medicine virus-free test-tube plantlet |
| CN108967192A (en) * | 2018-07-04 | 2018-12-11 | 商丘市农林科学院 | A kind of Sweetpotato Viruses Elimination bottle seedling acclimatization and transplants method |
| CN109874677A (en) * | 2019-04-16 | 2019-06-14 | 山东省烟台市农业科学研究院 | A kind of method of the direct transplantation of Sweetpotato Viruses Elimination test tube seedling |
| CN112314378A (en) * | 2020-10-26 | 2021-02-05 | 广西壮族自治区农业科学院 | Efficient sugarcane test-tube seedling transplanting method |
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| CN101611697B (en) * | 2009-07-13 | 2011-08-24 | 周玉玲 | Virus removal and rapid propagation technology of sweet potato variety 'Shangshu 19' |
| CN102138531A (en) * | 2011-04-26 | 2011-08-03 | 马宗新 | Rapid propagation technology and culture medium composition of Fuyang sweet potato 24 virus-free plantlet |
| CN102860259A (en) * | 2012-09-26 | 2013-01-09 | 海南省农业科学院粮食作物研究所 | High-efficiency industrial production method of virus-free tissue culture seedling of sweet potato |
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