CN103975723A - Sweet potato virus-free test tube seedling net house direct transplanting soil entering method - Google Patents
Sweet potato virus-free test tube seedling net house direct transplanting soil entering method Download PDFInfo
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- CN103975723A CN103975723A CN201410168985.9A CN201410168985A CN103975723A CN 103975723 A CN103975723 A CN 103975723A CN 201410168985 A CN201410168985 A CN 201410168985A CN 103975723 A CN103975723 A CN 103975723A
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Abstract
The invention relates to the technical field of plant test tube seedling outside transplanting, in particular to a sweet potato virus-free test tube seedling net house direct transplanting soil entering method, which comprises the following steps that sweet potato virus-free tissue culture is carried out in a test tube, when sweet potatoes grow to 5 to 6 leaves, sweet potato virus-free rooting seedlings are formed, the sweet potato virus-free rooting seedlings are directly planted into tree nursery land, soil covering and watering are carried out, a plastic film arch shed is adopted and built in time, in addition, a sun shielding net is added and covered, covering shade shielding and moisture preservation are carried out for 30 days, and the sun shielding net and the plastic film are removed. The sweet potato virus-free test tube seedling net house direct transplanting soil entering method has the advantages that the nutrition pot transplanting step in a conversional method is omitted, the operation process is simplified, in addition, the seedling survival time is also shortened, new leaves and new roots can grow out after 15 days of the transplanting, the seedlings can vigorously grow after 30 days, the survival rate is mainly and greatly improved, and the survival rate reaches more than 96 percent.
Description
Technical field
The present invention relates to plant test-tube plantlet and move technical field outward, specifically a kind of Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method.
Background technology
Sweet potato is important grain, feed, the raw material of industry and novel energy root crop, is also the bottom line crop that ensures China's grain security, and China plants 8,000 ten thousand mu throughout the year, and output accounts for the world and always produces more than 80%.But sweet potato is asexually propagated crop, be subject to the dip-dye of multiple virus and pathogen, cause kind of a sexual involution, yield reducation, quality declines.Report that at present virus and the viroids of infecting sweet potato have more than 20 to plant, especially Virus Diseases of Sweet Potato (SPVD) occurrence tendency is serious, it is reported that the aobvious disease plant of SPVD is compared with healthy plant underproduction 79-86%, utilize stem-tip tissue to cultivate and produce virus-free potato seedling, be to remove virus, recovery kind merit, improve the unique channel of tuber yield and quality.
Conventional transplanting method is that Sweetpotato Viruses Elimination test-tube plantlet is opened to bottle cap hardening 2 ~ 3 days in laboratory, then be transplanted to and be equipped with in the nutritive cube of Nutrition Soil+vermiculite+peat soil more than 30 days, pouring is transplanted nutrient solution and is taked moist keeping measures, and the detoxification test tube plantlet after surviving is transplanted to the field, garden in fly net canopy again.The method is in Sweetpotato Viruses Elimination test-tube seedling transplanting process, and group training seedling will fall to plant through twice, and survival rate is lower, and take a lot of work, time-consuming, cost is high, survival rate is low, is unfavorable for that the industrialization of Virus-free Sweetpotato is produced.
Summary of the invention
The problem that the present invention exists in order to solve existing Sweetpotato Viruses Elimination test-tube seedling transplanting method, provides a kind of Sweetpotato Viruses Elimination test-tube plantlet net canopy to be directly transplanted into indigenous method.
The present invention is achieved by the following technical solutions: Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method, the steps include: to carry out Sweetpotato Viruses Elimination tissue in test tube cultivates, grow to the seedling of taking root of 5～6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt and build plastic film shed in time, and add a cover sunshade net, cover the moisturizing 30 days of sheltering from heat or light, remove sunshade net and plastic film.
Further, blinding thickness is 2.5～3.0cm, and the soil moisture content after watering is 13～14%.Blinding is excessively thin, and the shoot root of taking root system is few with soil Exposure, easily lodging and be easily exposed to outside, cause the seedling dehydration of taking root withered dead; Blinding is blocked up, and rhizome and soil contact portion are too much, affect gas permeability, and the shoot root stem of taking root is easily at this position perish.Soil moisture content is greater than 14%, and humidity is excessive, and the seedling transplanting seedling time of taking root is long, the seedling that even can cause taking root because of anoxic, grow germ perish; Lower than 13%, Sweetpotato Viruses Elimination seedling is understood a large amount of dehydrations and is wilted gradually, and soil moisture is lower, and wilting speed is faster, and transplanting survival rate is lower.
Further, plastic film shed top is far from ground 30～40cm.Highly determined the size in canopy space, lower than 30cm, the seedling of taking root touches ceiling, easily scalds seedling; Higher than 40cm, humidity be difficult for to keep, and seedling dehydration is too fast and wilt.
Further, the temperature in plastic film shed is controlled between 17～32 ℃, and humidity remains between 70～85%, and the shading rate of sunshade net is 50～60%.Under above-mentioned environmental condition, fast slow seedling, photosynthesis, nutrient accumulation and the root growth and development of the seedling that is conducive to take root, improved transplanting survival rate, Sweetpotato Viruses Elimination seedling robust growth.
Further, cover the moisturizing 3rd～4 days of sheltering from heat or light, open plastic film shed and carry out secondary soil-covering, moisturizing; The 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; The 30th day, remove plastic film.According to the upgrowth situation of Sweetpotato Viruses Elimination seedling, reduce gradually the temperature and humidity in canopy, make it final consistent with temperature, the humidity of external environment, object is to temper gradually seedling, finally makes it to adapt to grown in field environment.
Sweetpotato Viruses Elimination test-tube plantlet net canopy of the present invention is directly transplanted into indigenous method, saved the step that in conventional method, nutritive cube is transplanted, not only simplified operating process, but also shortened transplanting seedling time, transplant and within 15 days, just grow afterwards young leaves, new root, after 30 days, with regard to vigorous growth, key is greatly to have improved survival rate, and survival rate reaches more than 96%.
Embodiment
In order to illustrate better, below by specific embodiment, the present invention is explained in detail the present invention, wherein the tissue of Sweetpotato Viruses Elimination test-tube plantlet is cultivated as this area routine techniques means.
embodiment 1
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method and utilizes shoot apical meristem culture technique, by Sweet Potato Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0 g/L+6-BA(6-benzylaminopurines) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, form strain after Brazilian morning-glory grafting detection is virus-free, in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium, carry out plantlet bud propagation.Grow to the seedling of taking root of 5 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt and build plastic film shed in time, and add a cover sunshade net, covers the moisturizing 30 days of sheltering from heat or light, and removes sunshade net and plastic film.
Blinding thickness is 2.7cm, and the soil moisture content after watering is 14%.Plastic film shed top is far from ground 40cm.Temperature in plastic film shed is controlled between 17～32 ℃, and humidity remains between 70～85%, and the shading rate of sunshade net is 55%.The covering moisturizing the 3rd day of sheltering from heat or light, opens plastic film shed and carries out secondary soil-covering, moisturizing; The 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; The 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into nursery lot Sweetpotato Viruses Elimination is 900 strains, survives 868 strains, survival rate 96.44% after 30 days.
embodiment 2
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method and utilizes shoot apical meristem culture technique, by Sweet Potato Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0 g/L+6-BA(6-benzylaminopurines) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, form strain after Brazilian morning-glory grafting detection is virus-free, in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium, carry out plantlet bud propagation.Grow to the seedling of taking root of 6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt and build plastic film shed in time, and add a cover sunshade net, covers the moisturizing 20 days of sheltering from heat or light, and removes sunshade net and plastic film.
Blinding thickness is 3.0cm, and the soil moisture content after watering is 13%.Plastic film shed top is far from ground 35cm.Temperature in plastic film shed is controlled between 17～32 ℃, and humidity remains between 70～85%, and the shading rate of sunshade net is 60%.The covering moisturizing the 3rd day of sheltering from heat or light, opens plastic film shed and carries out secondary soil-covering, moisturizing; The 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; The 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into nursery lot Sweetpotato Viruses Elimination is 325 strains, survives 312 strains, survival rate 96.00% after 30 days.
embodiment 3
Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method and utilizes shoot apical meristem culture technique, by Sweet Potato Duan Miaoxian 0.10%Hgcl
2sterilize after 8 ~ 10 minutes, adopt MS+ sucrose 30g/L+ agar 7.0 g/L+6-BA(6-benzylaminopurines) 0.25 ~ 2.0mg/L+NAA(methyl α-naphthyl acetate) 0.01mg/L) medium, induction stem apex (0.2 ~ 0.4mm) seedling differentiation, form strain after Brazilian morning-glory grafting detection is virus-free, in 1/4 macroelement+1/2 trace element (comprising vitamin, molysite)+1/2 white granulated sugar medium, carry out plantlet bud propagation.Grow to the seedling of taking root of 5 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot, blinding, watering, adopt and build plastic film shed in time, and add a cover sunshade net, covers the moisturizing 20 days of sheltering from heat or light, and removes sunshade net and plastic film.
Blinding thickness is 2.5cm, and the soil moisture content after watering is 13.5%.Plastic film shed top is far from ground 30cm.Temperature in plastic film shed is controlled between 17～32 ℃, and humidity remains between 70～85%, and the shading rate of sunshade net is 50%.The covering moisturizing the 3rd day of sheltering from heat or light, opens plastic film shed and carries out secondary soil-covering, moisturizing; The 7th day, hole drilling ventilation on plastic film shed, then strengthened air-vent gradually; The 30th day, remove plastic film.
The seedling quantity of taking root of directly planting in above-described embodiment into nursery lot Sweetpotato Viruses Elimination is 277 strains, survives 266 strains, survival rate 96.03% after 30 days.
Claims (5)
1. Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method, it is characterized in that, the steps include: to carry out Sweetpotato Viruses Elimination tissue in test tube cultivates, grow to the seedling of taking root of 5～6 leaf one-tenth Sweetpotato Viruses Eliminations, the seedling of taking root of Sweetpotato Viruses Elimination is directly planted into nursery lot to blinding, watering, adopt and build plastic film shed in time, and add a cover sunshade net, and cover the moisturizing 30 days of sheltering from heat or light, remove sunshade net and plastic film.
2. Sweetpotato Viruses Elimination test-tube plantlet net canopy according to claim 1 is directly transplanted into indigenous method, it is characterized in that, blinding thickness is 2.5～3.0cm, and the soil moisture content after watering is 13～14%.
3. Sweetpotato Viruses Elimination test-tube plantlet net canopy according to claim 2 is directly transplanted into indigenous method, it is characterized in that, plastic film shed top is far from ground 30～40cm.
4. Sweetpotato Viruses Elimination test-tube plantlet net canopy according to claim 3 is directly transplanted into indigenous method, it is characterized in that, the temperature in plastic film shed is controlled between 17～32 ℃, and humidity remains between 70～85%, and the shading rate of sunshade net is 50～60%.
5. Sweetpotato Viruses Elimination test-tube plantlet net canopy according to claim 4 is directly transplanted into indigenous method, it is characterized in that, covers the moisturizing 3rd～4 days of sheltering from heat or light, and opens plastic film shed and carries out secondary soil-covering, moisturizing; The 7th day, hole drilling ventilation on plastic film shed; The 30th day, remove plastic film.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410168985.9A CN103975723B (en) | 2014-04-25 | 2014-04-25 | Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method |
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| CN201410168985.9A CN103975723B (en) | 2014-04-25 | 2014-04-25 | Sweetpotato Viruses Elimination test-tube plantlet net canopy is directly transplanted into indigenous method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386144A (en) * | 2016-10-09 | 2017-02-15 | 江苏建康职业学院 | Cultivation method of traditional Chinese virus-free test-tube plantlets |
| CN108967192A (en) * | 2018-07-04 | 2018-12-11 | 商丘市农林科学院 | A kind of Sweetpotato Viruses Elimination bottle seedling acclimatization and transplants method |
| CN109874677A (en) * | 2019-04-16 | 2019-06-14 | 山东省烟台市农业科学研究院 | A kind of method of the direct transplantation of Sweetpotato Viruses Elimination test tube seedling |
| CN112314378A (en) * | 2020-10-26 | 2021-02-05 | 广西壮族自治区农业科学院 | Efficient sugarcane test-tube seedling transplanting method |
Citations (3)
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| CN101611697A (en) * | 2009-07-13 | 2009-12-30 | 周玉玲 | Sweet potato merchant 19 detoxifying fast breeding technique and cultivation material |
| CN102138531A (en) * | 2011-04-26 | 2011-08-03 | 马宗新 | Rapid propagation technology and culture medium composition of Fuyang sweet potato 24 virus-free plantlet |
| CN103202232A (en) * | 2012-09-26 | 2013-07-17 | 海南省农业科学院粮食作物研究所 | Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings |
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2014
- 2014-04-25 CN CN201410168985.9A patent/CN103975723B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101611697A (en) * | 2009-07-13 | 2009-12-30 | 周玉玲 | Sweet potato merchant 19 detoxifying fast breeding technique and cultivation material |
| CN102138531A (en) * | 2011-04-26 | 2011-08-03 | 马宗新 | Rapid propagation technology and culture medium composition of Fuyang sweet potato 24 virus-free plantlet |
| CN103202232A (en) * | 2012-09-26 | 2013-07-17 | 海南省农业科学院粮食作物研究所 | Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings |
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| 孙光泽等: "甘薯脱毒与快繁技术", 《农业与技术》, vol. 27, no. 04, 31 August 2007 (2007-08-31), pages 124 - 126 * |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106386144A (en) * | 2016-10-09 | 2017-02-15 | 江苏建康职业学院 | Cultivation method of traditional Chinese virus-free test-tube plantlets |
| CN106386144B (en) * | 2016-10-09 | 2020-03-31 | 江苏建康职业学院 | Cultivation method of traditional Chinese medicine virus-free test-tube plantlet |
| CN108967192A (en) * | 2018-07-04 | 2018-12-11 | 商丘市农林科学院 | A kind of Sweetpotato Viruses Elimination bottle seedling acclimatization and transplants method |
| CN109874677A (en) * | 2019-04-16 | 2019-06-14 | 山东省烟台市农业科学研究院 | A kind of method of the direct transplantation of Sweetpotato Viruses Elimination test tube seedling |
| CN112314378A (en) * | 2020-10-26 | 2021-02-05 | 广西壮族自治区农业科学院 | Efficient sugarcane test-tube seedling transplanting method |
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| CN103975723B (en) | 2016-01-20 |
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