CN104303765A - High-yield planting method for noble dendrobium - Google Patents
High-yield planting method for noble dendrobium Download PDFInfo
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- CN104303765A CN104303765A CN201410528102.0A CN201410528102A CN104303765A CN 104303765 A CN104303765 A CN 104303765A CN 201410528102 A CN201410528102 A CN 201410528102A CN 104303765 A CN104303765 A CN 104303765A
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- 240000004638 Dendrobium nobile Species 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 24
- 201000010099 disease Diseases 0.000 claims abstract description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 7
- 241000196324 Embryophyta Species 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 18
- 239000001963 growth medium Substances 0.000 claims description 15
- 230000006698 induction Effects 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 15
- 210000003608 fece Anatomy 0.000 claims description 12
- 230000001954 sterilising effect Effects 0.000 claims description 12
- 229920001817 Agar Polymers 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- 239000010871 livestock manure Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 210000004185 liver Anatomy 0.000 claims description 7
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 6
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 6
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 6
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 6
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 6
- 239000003337 fertilizer Substances 0.000 claims description 6
- 238000005286 illumination Methods 0.000 claims description 6
- 238000000338 in vitro Methods 0.000 claims description 6
- 239000006870 ms-medium Substances 0.000 claims description 6
- 230000008929 regeneration Effects 0.000 claims description 6
- 238000011069 regeneration method Methods 0.000 claims description 6
- 241000238631 Hexapoda Species 0.000 claims description 5
- 238000009333 weeding Methods 0.000 claims description 4
- 239000002023 wood Substances 0.000 claims description 4
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 claims description 3
- 235000020197 coconut milk Nutrition 0.000 claims description 3
- 239000000306 component Substances 0.000 claims description 3
- 230000006835 compression Effects 0.000 claims description 3
- 238000007906 compression Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 230000004720 fertilization Effects 0.000 claims description 3
- 239000002609 medium Substances 0.000 claims description 3
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 3
- 238000010899 nucleation Methods 0.000 claims description 3
- 239000002686 phosphate fertilizer Substances 0.000 claims description 3
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 3
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 3
- 235000011151 potassium sulphates Nutrition 0.000 claims description 3
- 239000004576 sand Substances 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- -1 sugared 35g/L Substances 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 239000002426 superphosphate Substances 0.000 claims description 3
- 238000002054 transplantation Methods 0.000 claims description 3
- 238000009423 ventilation Methods 0.000 claims description 3
- 240000008790 Musa x paradisiaca Species 0.000 claims 2
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 238000003306 harvesting Methods 0.000 abstract 1
- 241000234295 Musa Species 0.000 description 4
- 241001523681 Dendrobium Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 241000208140 Acer Species 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000026010 Dendrobium candidum Species 0.000 description 1
- 241000229499 Dendrobium loddigesii Species 0.000 description 1
- 208000015220 Febrile disease Diseases 0.000 description 1
- 235000019738 Limestone Nutrition 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000006028 limestone Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a high-yield planting method for noble dendrobium. The method comprises the steps of carrying out isolated seedling culture, carrying out transplanted culture and harvesting. Through the manner, the high-yield planting method for the noble dendrobium, disclosed by the invention, has the advantages that the yield of the noble dendrobium can be greatly increased, seedlings can be effectively prevented from being threatened by diseases and pests, and the quality of the noble dendrobium is improved.
Description
Technical field
The present invention relates to method for growing dendrobium field, particularly relate to a kind of high-yield planting method of the stem of noble dendrobium.
Background technology
The stem of noble dendrobium is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family.Be used as medicine with stem.There is tonifying-Yin and nourishing-stomach, effect of clearing heat and promoting fluid and improving kidney and eyes, control consumption of YIN caused by febrile disease, dry polydipsia, food is few retches, abnormal heat such as not to move back at the disease, be distributed in Sichuan, Chongqing, Guizhou, Yunnan, Hubei, Guangdong, the provinces and regions such as Guangxi, mostly be wild, also a small amount of cultivation is had, make the medicinal stem of noble dendrobium in China and have kind more than 20, common are: HERBA DENDROBII, dendrobium candidum, HERBA DENDROBII, dendrobium loddigesii Rolfe, there is crab blue at the stem of noble dendrobium of Sichuan Hejiang cultivation, fish maw is blue, the blue germination and growth of crab is fast, the index of aridity and output are all higher, with " the western maple bucket " of stem of noble dendrobium processing for stem of noble dendrobium treasure, expensive.
But the implantation methods of the stem of noble dendrobium is also immature now, and output is lower, and be difficult to the quality ensureing the stem of noble dendrobium, so people need the method for growing dendrobium more met the demands.
Summary of the invention
The technical problem that the present invention mainly solves is to provide a kind of high-yield planting method of the stem of noble dendrobium, has the advantages such as output is high, quality better, simultaneously method for growing dendrobium application and universal on have market prospects widely.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
There is provided a kind of high-yield planting method of the stem of noble dendrobium, its step comprises:
One. in vitro nursery: configuration adventitious bud induction culture base, and adventitious bud induction culture base is carried out disinfection sterilization; The blade without damage by disease and insect of the selection stem of noble dendrobium and tender stem are as explant, and sheared off by explant with the cutter of sterilizing, the length of described explant is 0.5cm-1cm; Explant is put into adventitious bud induction culture base to cultivate; After 15-20 days, configuration proliferated culture medium, and proliferated culture medium is carried out disinfection sterilization; Select the explant growing sprouting, the explant with sprouting is put in proliferated culture medium, cultivate 10-15 days; Configuration root media, and root media is carried out disinfection sterilization; Select to grow the explant of clump bud, and put it in root media and cultivate;
Two. transplant: the plantation field selecting moistening, the low light level, ventilation, sandy soil, and combine deep ploughing basal dressing; In plantation, Tanaka makes wide 30-40cm, long 100-120cm, the furrow of high 15-17cm; Plantation field is taken the cool canopy of 1.5-2.0m, and the temperature in cool canopy is 15-30 DEG C, and humidity is 60%-90%; In plantation, Tanaka sows bark, wood chip and liver moss uniformly; After 20-30 days, select the explant with flourishing root system as regeneration plant, and wrap up one deck liver moss at the root of regeneration plant; According to the seeding row spacing of 10-20cm, by Transplantation of Regenerated Plantlets in plantation field, with fine sand or little gravel landfill to 7-10cm, after compression, water normal root water; After plantation, Second Year starts, and in the 3-4 month and the 10-11 month, carries out a weeding and imposes the supernatant of well-rotted farmyard manure; Annual between the 5-10 month, every 1-2 month, be that the superphosphate of 2% or the potassium sulfate of 1% carry out foliage fertilization with mass ratio; Before germinateing in annual spring or gather the stem of noble dendrobium time, the old branch of part and deadwood should be cut off, and grow overstocked stem branch, make shade rate be 55%-65%;
Three. gather: after cultivation 2-3, when gathering between the 10-4 month, from basal part of stem, old plant is sheared off with scissors or reaping hook, retain tender strain.
In a preferred embodiment of the present invention, when cultured in vitro, cultivation temperature 25 DEG C ~ 28 DEG C, illumination every day 9-10 hour, intensity of illumination is 1800 ~ 1900lx.
In a preferred embodiment of the present invention, the composition of described base manure and composition and component thereof comprise: the nitrogenous fertilizer of the fertile mud of the cow dung of the oil cake of 50-80 part, the bean dregs of 80-100 part, 40-60 part, the pig manure of 40-60 part, 60-80 part, the attachment fertilizer of 20-30 part, the phosphate fertilizer of 10-20 part and 5-10 part.
In a preferred embodiment of the present invention, the composition of described adventitious bud induction culture base comprises: MS medium, sugared 35g/L, agar 3.7g/L and coconut milk 100ml/L.
In a preferred embodiment of the present invention, the composition of described proliferated culture medium comprises: MS medium, NAA 0.1g/L, 6-BA 0.2g/L, proline 0.3g/L, sugared 35g/L, banana 100g/L and agar 3.7g/L.
In a preferred embodiment of the present invention, the composition of described root media comprises: 1/2MS medium, NAA 0.2g/L, 6-BA 0.1g/L, proline 0.3g/L, sugared 35g/L, agar 3.7g/L and banana 100g/L.
In a preferred embodiment of the present invention, the pH value of described adventitious bud induction culture base, described proliferated culture medium and described root media is 5.6-5.8.
In a preferred embodiment of the present invention, attachment fertilizer comprise in oil foot, pig hair, bean dregs or human hair one or more.
The invention has the beneficial effects as follows: the output that not only can improve the stem of noble dendrobium greatly, and can effectively prevent seedling to be subject to the threat of damage by disease and insect, improve the quality of the stem of noble dendrobium.
Embodiment
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
The embodiment of the present invention comprises:
A high-yield planting method for the stem of noble dendrobium, its step comprises:
One. in vitro nursery: configuration adventitious bud induction culture base, and adventitious bud induction culture base is carried out disinfection sterilization;
The blade without damage by disease and insect of the selection stem of noble dendrobium and tender stem are as explant, and sheared off by explant with the cutter of sterilizing, the length of described explant is 0.5cm-1cm;
Explant is put into adventitious bud induction culture base to cultivate;
After 15-20 days, configuration proliferated culture medium, and proliferated culture medium is carried out disinfection sterilization;
Select the explant growing sprouting, the explant with sprouting is put in proliferated culture medium, cultivate 10-15 days;
Configuration root media, and root media is carried out disinfection sterilization;
Select to grow the explant of clump bud, and put it in root media and cultivate;
Two. transplant: the plantation field selecting moistening, the low light level, ventilation, sandy soil, and combine deep ploughing basal dressing;
In plantation, Tanaka makes wide 30-40cm, long 100-120cm, the furrow of high 15-17cm;
Plantation field is taken the cool canopy of 1.5-2.0m, and the temperature in cool canopy is 15-30 DEG C, and humidity is 60%-90%, and sunny slope hangs a straw screen or mat, in order to adjustment temp. and humidity and penetrating fresh air, and often keeps the moistening of furrow face;
In plantation, Tanaka sows bark, wood chip and liver moss uniformly, because the stem of noble dendrobium is epiphyte, attached master is larger to its growth effect, the stem of noble dendrobium absorbs nutrient and moisture content in atmosphere by exposed aerial root, so need for the stem of noble dendrobium provides enough objects of growing nonparasitically upon another plant, and bark, wood chip and liver moss on the basis of improving stem of noble dendrobium survival rate, can improve the medical value of the stem of noble dendrobium to greatest extent, can select to sow limestone particle simultaneously;
After 20-30 days, select the explant with flourishing root system as regeneration plant, and wrap up one deck liver moss at the root of regeneration plant;
According to the seeding row spacing of 10-20cm, by Transplantation of Regenerated Plantlets in plantation field, with fine sand or little gravel landfill to 7-10cm, after compression, water normal root water;
After plantation, Second Year starts, in the 3-4 month and the 10-11 month, carry out a weeding and impose the supernatant of well-rotted farmyard manure, fertilising water time and number of times are mainly determined according to canopy humidity, Shaoshi when canopy humidity is large, when suffering from a severe drought, duty is executed, Shaoshi during flood, note epidemic disaster change in canopy, grasp flexibly;
During weeding by long between stem of noble dendrobium strain and around weeds and dry branches and fallen leaves remove and then can;
Annual between the 5-10 month, every 1-2 month, be that the superphosphate of 2% or the potassium sulfate of 1% carry out foliage fertilization with mass ratio;
Before germinateing in annual spring or gather the stem of noble dendrobium time, the old branch of part and deadwood should be cut off, and grow overstocked stem branch, make shade rate be 55%-65%;
After the stem of noble dendrobium is planted 5 years, plant sprouts a lot, and old root is dead, and matrix is rotted, and infection process, makes plant strain growth bad, therefore should carry out turning over the root and stem of certain plants according to growing state, removes dry and decayed old root, carries out plant division, cultivate separately, to promote growth and the increasing both production and income of plant;
Three. gather: after cultivation 2-3, gather between the 10-4 month, now, the stem of noble dendrobium stops growing, branch stem is solid full, and water content is few, and the index of aridity is high, crudy is good, from basal part of stem, old plant is sheared off with scissors or reaping hook, retain tender strain, to gather in the crops continuously the coming year, reach plantation in a year, the object of being benefited for many years.
When cultured in vitro, cultivation temperature 25 DEG C ~ 28 DEG C, illumination every day 9-10 hour, intensity of illumination is 1800 ~ 1900lx.
The composition of described base manure and composition and component thereof comprise: the nitrogenous fertilizer of the fertile mud of the cow dung of the oil cake of 50-80 part, the bean dregs of 80-100 part, 40-60 part, the pig manure of 40-60 part, 60-80 part, the attachment fertilizer of 20-30 part, the phosphate fertilizer of 10-20 part and 5-10 part.
The composition of described adventitious bud induction culture base comprises: MS medium, sugared 35g/L, agar 3.7g/L and coconut milk 100ml/L.
The composition of described proliferated culture medium comprises: MS medium, NAA 0.1g/L, 6-BA 0.2g/L, proline 0.3g/L, sugared 35g/L, banana 100g/L and agar 3.7g/L.
The composition of described root media comprises: 1/2MS medium, NAA 0.2g/L, 6-BA 0.1g/L, proline 0.3g/L, sugared 35g/L, agar 3.7g/L and banana 100g/L.
The pH value of described adventitious bud induction culture base, described proliferated culture medium and described root media is 5.6-5.8.
Attachment fertilizer comprise in oil foot, pig hair, bean dregs or human hair one or more.
The beneficial effect of the high-yield planting method of the stem of noble dendrobium of the present invention is: the output that not only can improve the stem of noble dendrobium greatly, and can effectively prevent seedling to be subject to the threat of damage by disease and insect, improves the quality of the stem of noble dendrobium.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every utilize description of the present invention to do equivalent structure or equivalent flow process conversion; or be directly or indirectly used in other relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (8)
1. a high-yield planting method for the stem of noble dendrobium, it is characterized in that, step comprises:
One. in vitro nursery:
Configuration adventitious bud induction culture base, and adventitious bud induction culture base is carried out disinfection sterilization;
The blade without damage by disease and insect of the selection stem of noble dendrobium and tender stem are as explant, and sheared off by explant with the cutter of sterilizing, the length of described explant is 0.5cm-1cm;
Explant is put into adventitious bud induction culture base to cultivate;
After 15-20 days, configuration proliferated culture medium, and proliferated culture medium is carried out disinfection sterilization;
Select the explant growing sprouting, the explant with sprouting is put in proliferated culture medium, cultivate 10-15 days;
Configuration root media, and root media is carried out disinfection sterilization;
Select to grow the explant of clump bud, and put it in root media and cultivate;
Two. transplant:
Select the plantation field of moistening, the low light level, ventilation, sandy soil, and combine deep ploughing basal dressing;
In plantation, Tanaka makes wide 30-40cm, long 100-120cm, the furrow of high 15-17cm;
Plantation field is taken the cool canopy of 1.5-2.0m, and the temperature in cool canopy is 15-30 DEG C, and humidity is 60%-90%;
In plantation, Tanaka sows bark, wood chip and liver moss uniformly;
After 20-30 days, select the explant with flourishing root system as regeneration plant, and wrap up one deck liver moss at the root of regeneration plant;
According to the seeding row spacing of 10-20cm, by Transplantation of Regenerated Plantlets in plantation field, with fine sand or little gravel landfill to 7-10cm, after compression, water normal root water;
After plantation, Second Year starts, and in the 3-4 month and the 10-11 month, carries out a weeding and imposes the supernatant of well-rotted farmyard manure;
Annual between the 5-10 month, every 1-2 month, be that the superphosphate of 2% or the potassium sulfate of 1% carry out foliage fertilization with mass ratio;
Before germinateing in annual spring or gather the stem of noble dendrobium time, the old branch of part and deadwood should be cut off, and grow overstocked stem branch, make shade rate be 55%-65%;
Three. gather:
After cultivation 2-3, when gathering between the 10-4 month, from basal part of stem, old plant is sheared off with scissors or reaping hook, retain tender strain.
2. the high-yield planting method of the stem of noble dendrobium according to claim 1, is characterized in that, when cultured in vitro, cultivation temperature 25 DEG C ~ 28 DEG C, illumination every day 9-10 hour, intensity of illumination is 1800 ~ 1900lx.
3. the high-yield planting method of the stem of noble dendrobium according to claim 1, it is characterized in that, the composition of described base manure and composition and component thereof comprise: the nitrogenous fertilizer of the fertile mud of the cow dung of the oil cake of 50-80 part, the bean dregs of 80-100 part, 40-60 part, the pig manure of 40-60 part, 60-80 part, the attachment fertilizer of 20-30 part, the phosphate fertilizer of 10-20 part and 5-10 part.
4. the high-yield planting method of the stem of noble dendrobium according to claim 1, is characterized in that, the composition of described adventitious bud induction culture base comprises: MS medium, sugared 35g/L, agar 3.7g/L and coconut milk 100ml/L.
5. the high-yield planting method of the stem of noble dendrobium according to claim 1, is characterized in that, the composition of described proliferated culture medium comprises: MS medium, NAA 0.1g/L, 6-BA 0.2g/L, proline 0.3g/L, sugared 35g/L, banana 100g/L and agar 3.7g/L.
6. the high-yield planting method of the stem of noble dendrobium according to claim 1, it is characterized in that, the composition of described root media comprises: 1/2MS medium, NAA 0.2g/L, 6-BA 0.1g/L, proline 0.3g/L, sugared 35g/L, agar 3.7g/L and banana 100g/L.
7. the high-yield planting method of the stem of noble dendrobium according to claim 4 or 5 or 6, is characterized in that, the pH value of described adventitious bud induction culture base, described proliferated culture medium and described root media is 5.6-5.8.
8. the high-yield planting method of the stem of noble dendrobium according to claim 3, is characterized in that, attachment fertilizer comprise in oil foot, pig hair, bean dregs or human hair one or more.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107494221A (en) * | 2017-08-23 | 2017-12-22 | 赤水市信天中药产业开发有限公司 | A kind of method for potted of HERBA DENDROBII |
| CN108812165A (en) * | 2018-06-08 | 2018-11-16 | 安徽三生缘时代生物科技有限公司 | A kind of high-yield planting method of dendrobium nobile |
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| CN104904593A (en) * | 2015-05-19 | 2015-09-16 | 安徽霍山鹏泽生物科技有限公司 | Dendrobium planting method |
| CN107494221A (en) * | 2017-08-23 | 2017-12-22 | 赤水市信天中药产业开发有限公司 | A kind of method for potted of HERBA DENDROBII |
| CN108812165A (en) * | 2018-06-08 | 2018-11-16 | 安徽三生缘时代生物科技有限公司 | A kind of high-yield planting method of dendrobium nobile |
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