CN104756955A - Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method - Google Patents
Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method Download PDFInfo
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Abstract
Disclosed is an Aphidius gifuensis Ashmaed and Encarsia formosa simultaneous breeding method. Tobaccos or eggplants which is or are poor in insect resistance is or are utilized as host plants of myzus persicae and bemisia tabaci, the myzus persicae and the bemisia tabaci are bred together in a simultaneous breeding chamber after corresponding pest species and bee species are obtained through classified breeding on the myzus persicae, Aphidius gifuensis Ashmaed, the bemisia tabaci and Encarsia Formosa, the Aphidius gifuensis Ashmaed and the Encarsia Formosa are inoculated on simultaneous breeding chamber plants, and accordingly the simultaneous breeding of the Aphidius gifuensis Ashmaed and the Encarsia Formosa is implemented. The breeding utilization rate of the host plants is greatly improved, more than 10000 Aphidius gifuensis Ashmaed and more than 12000 Encarsia Formosa can be simultaneously bred on every host plant in the simultaneous breeding chamber, and the breeding efficiency is improved by 40 to 60% under the same facility condition in comparison with the common method. The two types of natural parasitic wasps can be bred simultaneously and accordingly the problem of the simultaneous harm of the two types of pests of the myzus persicae and the bemisia tabaci to the actual production can be effectively solved, the control effect is good, the efficiency is high, and the technology of prevention and control on multiple pests is improved due to the biological diversity.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry injurious insect, particularly aphidius gifuensis together with Encarsia formosa with numerous method.
Background technology
Cigarette aphid colonizes on the plant of the crops such as Solanaceae, Cruciferae, composite family, pulse family, Chenopodiaceae, Convolvulaceae, the rose family, if it becomes the juice in aphid sucking plant body, make leaf curl, flavescence, stem and bennet distortion, deformity, plant is short and small, spread disease viral disease in sucking process, and excretion honeydew brings out tobacco leaf dark mildew.Cigarette aphid endangers larger in agricultural and forestry production.
Aleyrodid is as the worldwide insect of one, host range is extensive, known nearly 112 sections 653 kinds, the adult of aleyrodid and nymph utilize rasping-sucking mouthparts sucking plant juice, make plant leaf chlorisis, flavescence, wilting, bring out and propagate mycosis, virus disease, the harm on the crops such as flowers, fruit tree, medicinal material, tobacco is very serious.
In recent years along with the development of biological prevention, people's continuous research and utilization aphidius gifuensis control cigarette aphid, utilizes Encarsia formosa to prevent and treat aleyrodid, achieves certain effect in the technical field of the insect parasite controlling disease worm of artificial propagation single variety; In actual production, run into cigarette aphid and aleyrodid two kinds of insects when crops being endangered simultaneously, have employed artificial propagation parasitic wasp being carried out to single variety, field is discharged into after breeding aphidius gifuensis and Encarsia formosa respectively, single variety parasitic wasp breeds that the Prevention Technique cycle is long, efficiency is low, operation is many, cost is high, cigarette aphid and aleyrodid simultaneously damage to crops time control efficiency greatly reduce, explore, excavate that to carry out the technology of biological synthesis control to various pests very urgent.
Summary of the invention
In agricultural production, for cigarette aphid and aleyrodid two kinds of insects simultaneously damage to crops time, single variety parasitic wasp raising technology is adopted to breed aphidius gifuensis and Encarsia formosa respectively in prior art, control cigarette aphid and aleyrodid two kinds of insects, have that breeding cycle is long, efficiency is low, operation is many, cost is high, the problem that control efficiency reduces greatly, provide a kind of aphidius gifuensis with numerous method together with Encarsia formosa, the implementation step of the method comprises:
(1) prepare to breed condition: in greenhouse, be separated out the close cigarette aphid aphid kind room of size, aleyrodid worm kind room, cocoon honeybee honeybee kind room, each 1 of Encarsia formosa honeybee kind room, with numerous room 5-10, the surrounding of each room above-mentioned all uses the airtight isolation of 80 object fly net;
(2) cigarette aphid aphid kind is bred: in cigarette aphid aphid kind room, cultivate tobacco or eggplant seedling, tobacco or eggplant in cigarette aphid aphid kind room conveniently water and fertilizer management natural lighting cultivate 15 days, tobacco in cigarette aphid aphid kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in cigarette aphid aphid kind room; Cigarette aphid is gathered to field, the cigarette aphid collected in field is inoculated on tobacco in cigarette aphid aphid kind room or eggplant plant, keep cigarette aphid aphid kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco of cigarette aphid in cigarette aphid aphid kind room or eggplant plant, the cigarette aphid quantity of in cigarette aphid aphid kind room, every strain tobacco or eggplant plant breeding after 10-15 days reaches more than 1000;
(3) aphidius gifuensis honeybee kind is bred: after cigarette aphid aphid kind indoor growing tobacco or eggplant reach 10 days in step (2), tobacco or eggplant is cultivated in cocoon honeybee honeybee kind room, tobacco or eggplant in cocoon honeybee honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, tobacco in cocoon honeybee honeybee kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in cocoon honeybee honeybee kind room; The blade with cigarette aphid is won from the tobacco cigarette aphid aphid kind room or eggplant plant, the cigarette aphid won on blade is inoculated on tobacco in cocoon honeybee honeybee kind room or eggplant plant, every strain tobacco or eggplant plant inoculate cigarette aphid 15, keep cocoon honeybee honeybee kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco of cigarette aphid in cocoon honeybee honeybee kind room or eggplant plant, the cigarette aphid quantity that after 5-10 days, in cocoon honeybee honeybee kind room, every strain tobacco or eggplant plant breed reaches 100-150 head; The stiff aphid of aphidius gifuensis is gathered or aphidius gifuensis becomes honeybee to field, the stiff aphid of the aphidius gifuensis that collected in field or aphidius gifuensis become honeybee to be inoculated on tobacco in cocoon honeybee honeybee kind room or eggplant plant, inoculative proportion according to honeybee aphid than 1:50-100, cocoon honeybee sex ration 1:0.5-2.0, aphidius gifuensis carries out growth reproduction 15 days in cocoon honeybee honeybee kind room, obtains aphidius gifuensis honeybee kind;
(4) aleyrodid worm kind is bred: cigarette aphid aphid kind indoor growing tobacco or eggplant seedling are on the same day in step (2), tobacco or eggplant seedling on cultivating in aleyrodid worm kind room, tobacco or eggplant seedling in aleyrodid worm kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco cultivated in aleyrodid worm kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant seedling continue cultivation 5 days in aleyrodid worm kind room; Bemisia tabaci or Bemisia tabaci Nymph is gathered to field, the Bemisia tabaci collected in field or Bemisia tabaci Nymph are inoculated on tobacco in aleyrodid worm kind room or eggplant plant, keep aleyrodid worm kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on tobacco in aleyrodid worm kind room of Bemisia tabaci or Bemisia tabaci Nymph or eggplant plant, the Bemisia tabaci quantity of in aleyrodid worm kind room, every strain tobacco or eggplant plant breeding after 10-15 days reaches more than 200;
(5) Encarsia formosa honeybee kind is bred: after aleyrodid worm kind indoor growing tobacco or eggplant seedling reach 10 days in step (4), tobacco or eggplant seedling is cultivated in Encarsia formosa honeybee kind room, tobacco or eggplant in Encarsia formosa honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco cultivated in Encarsia formosa honeybee kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in Encarsia formosa honeybee kind room, be inoculated on tobacco in Encarsia formosa honeybee kind room or eggplant plant by breeding the Bemisia tabaci obtained in aleyrodid worm kind room, every strain tobacco or eggplant plant inoculate Bemisia tabaci 15, keep Encarsia formosa honeybee kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco of Bemisia tabaci in Encarsia formosa honeybee kind room or eggplant plant, the Bemisia tabaci Nymph quantity of in Encarsia formosa honeybee kind room, every strain tobacco or eggplant plant breeding after 10 days reaches 20, Encarsia formosa or the black pupa of Encarsia formosa is gathered to field, the Encarsia formosa collected in field or the black pupa of Encarsia formosa are inoculated on tobacco in Encarsia formosa honeybee kind room or eggplant plant, inoculative proportion compares 1:20-50 according to honeybee worm, Encarsia formosa carries out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtain Encarsia formosa honeybee kind,
(6) cigarette aphid together with Bemisia tabaci with numerous: after Encarsia formosa honeybee kind indoor growing tobacco or eggplant reach 15 days in the step (5), each with numerous room in cultivate tobacco or eggplant seedling, tobacco or eggplant in numerous room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco cultivated in numerous room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in numerous room, from aleyrodid worm kind room, collect Bemisia tabaci adults or Bemisia tabaci Nymph is inoculated into on the tobacco of numerous indoor or eggplant plant, every strain tobacco or eggplant plant inoculate 30 Bemisia tabaci, keep with numerous indoor temperature 24-30 DEG C, humidity 40%-70%, natural lighting, after 5 days, from cigarette aphid aphid kind room, gather cigarette aphid is inoculated into on the tobacco of numerous indoor or eggplant plant, every strain tobacco or eggplant plant inoculate 20 cigarette aphids, cigarette aphid is breeding with numerous indoor growing together with Bemisia tabaci, within every 3-5 days, once manually shake plant leaf to the tobacco of numerous indoor or eggplant, Bemisia tabaci by even for ovum real estate on the blade of tobacco or eggplant plant, cigarette aphid and Bemisia tabaci are being 15 days with numerous time controling together with numerous indoor,
(7) aphidius gifuensis together with Encarsia formosa with numerous: cigarette aphid and Bemisia tabaci together with numerous indoor with numerous 15 days after, from Encarsia formosa honeybee kind room, collect Encarsia formosa becomes honeybee or the black pupa of Encarsia formosa to be inoculated into numerous indoor, inoculum concentration compares 1:30-60 according to Encarsia formosa and Bemisia tabaci worm's ovum, after 4 days, from cocoon honeybee honeybee kind room, collect aphidius gifuensis becomes honeybee or the stiff aphid of aphidius gifuensis to be inoculated into numerous indoor, inoculum concentration compares 1:50-100 according to aphidius gifuensis and cigarette aphid, keep with numerous indoor temperature 24-30 DEG C, humidity 40%-80%, fine day 11:00-17:00 time period shading rate be 70% sunshade net carry out shading, all the other time keeps natural lighting condition, after 15 days, the black pupa of a large amount of Encarsia formosas and the stiff aphid of aphidius gifuensis is being obtained with breeding simultaneously on the tobacco of numerous indoor or eggplant plant leaf,
The present invention utilizes the tobacco of resistance to insects difference or Eggplant Varieties as the host plant of cigarette aphid and Bemisia tabaci, by to cigarette aphid, aphidius gifuensis, Bemisia tabaci, Encarsia formosa carries out classifying after breeding obtains corresponding worm kind honeybee kind, cigarette aphid is being carried out and Bemisia tabaci is cooperatively bred with numerous indoor, then aphidius gifuensis and Encarsia formosa is being inoculated with on the plant of numerous room, realize aphidius gifuensis and Encarsia formosa with numerous, the breeding and utilizing rate of host plant of the present invention is adopted greatly to improve, the breeding effect of aphidius gifuensis and Encarsia formosa is good, simultaneously numerously aphidius gifuensis 10000 remainder can be obtained with strain host plant every in numerous room, Encarsia formosa 12000 remainder, identical facility condition is of the present invention breeds efficiency than the efficiency of breeding of carrying out single variety respectively and improves 40-60%, the present invention simultaneously numerously can obtain two kinds of natural enemy parasitic wasps, effectively can solve the problem that in actual production, cigarette aphid and Bemisia tabaci two kinds of insects are caused harm simultaneously, control efficiency is good, efficiency is high, the biological prevention of bio-diversity lifting to various pests can be made full use of.
Embodiment
embodiment 1:aphidius gifuensis is with a numerous method together with Encarsia formosa, and the implementation step of the method comprises:
(1) prepare breed condition: be separated out in greenhouse length and width be 4 meters cigarette aphid aphid kind room, aleyrodid worm kind room, cocoon honeybee honeybee kind room, each 1 of Encarsia formosa honeybee kind room, with 5, numerous room, the surrounding of each room above-mentioned all uses the airtight isolation of 80 object fly net;
(2) cigarette aphid aphid kind is bred: in cigarette aphid aphid kind room, cultivate tobacco, tobacco in cigarette aphid aphid kind room conveniently water and fertilizer management natural lighting cultivate 15 days, tobacco plant in cigarette aphid aphid kind room sprays 800 times of carbendazols, and then tobacco continues cultivation 5 days in cigarette aphid aphid kind room; Cigarette aphid is gathered to field, the cigarette aphid collected in field is inoculated on the tobacco plant in cigarette aphid aphid kind room, keep cigarette aphid aphid kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco plant of cigarette aphid in cigarette aphid aphid kind room, the cigarette aphid quantity of in cigarette aphid aphid kind room, every strain tobacco plant being bred after 10 days reaches more than 1000;
(3) aphidius gifuensis honeybee kind is bred: after cigarette aphid aphid kind indoor growing tobacco reaches 10 days in step (2), tobacco is cultivated in cocoon honeybee honeybee kind room, tobacco in cocoon honeybee honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, tobacco plant in cocoon honeybee honeybee kind room sprays 800 times of carbendazols, and then tobacco continues cultivation 5 days in cocoon honeybee honeybee kind room, the blade with cigarette aphid is won from the tobacco plant cigarette aphid aphid kind room, the cigarette aphid won on blade is inoculated on the tobacco plant in cocoon honeybee honeybee kind room, every strain tobacco plant is inoculated cigarette aphid 15, keep cocoon honeybee honeybee kind indoor temperature 24 DEG C, humidity 30%%, natural lighting, growth and breeding on the tobacco plant of cigarette aphid in cocoon honeybee honeybee kind room, the cigarette aphid quantity of in cocoon honeybee honeybee kind room, every strain tobacco plant being bred after 5 days reaches 100, the stiff aphid of aphidius gifuensis is gathered to field, the stiff aphid of the aphidius gifuensis collected in field is inoculated on the tobacco plant in cocoon honeybee honeybee kind room, inoculative proportion compares 1:50 according to honeybee aphid, cocoon honeybee sex ration 1:0.5, aphidius gifuensis carries out growth reproduction 15 days in cocoon honeybee honeybee kind room, obtain aphidius gifuensis honeybee kind,
(4) aleyrodid worm kind is bred: cigarette aphid aphid kind indoor growing tobacco on the same day in step (2), tobacco is cultivated in aleyrodid worm kind room, tobacco in aleyrodid worm kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco plant of cultivating in aleyrodid worm kind room sprays 800 times of carbendazols, and then tobacco continues cultivation 5 days in aleyrodid worm kind room; Bemisia tabaci is gathered to field, the Bemisia tabaci collected in field is inoculated on the tobacco plant in aleyrodid worm kind room, keep aleyrodid worm kind indoor temperature 24 DEG C, humidity 30%, natural lighting, growth and breeding on the tobacco plant of Bemisia tabaci in aleyrodid worm kind room, the Bemisia tabaci quantity of in aleyrodid worm kind room, every strain tobacco plant being bred after 10 days reaches more than 200;
(5) Encarsia formosa honeybee kind is bred: after aleyrodid worm kind indoor growing tobacco reaches 10 days in step (4), tobacco is cultivated in Encarsia formosa honeybee kind room, tobacco in Encarsia formosa honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco plant of cultivating in Encarsia formosa honeybee kind room sprays 800 times of carbendazols, and then tobacco continues cultivation 5 days in Encarsia formosa honeybee kind room, to breed on tobacco plant that the Bemisia tabaci obtained is inoculated in Encarsia formosa honeybee kind room in aleyrodid worm kind room, every strain tobacco plant is inoculated Bemisia tabaci 15, keep Encarsia formosa honeybee kind indoor temperature 24 DEG C, humidity 30%, natural lighting, growth and breeding on the tobacco plant of Bemisia tabaci in Encarsia formosa honeybee kind room, the Bemisia tabaci Nymph quantity of in Encarsia formosa honeybee kind room, every strain tobacco plant being bred after 10 days reaches 20, Encarsia formosa is gathered to field, the Encarsia formosa collected in field is inoculated on the tobacco plant in Encarsia formosa honeybee kind room, inoculative proportion compares 1:20 according to honeybee worm, Encarsia formosa carries out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtain Encarsia formosa honeybee kind,
(6) cigarette aphid together with Bemisia tabaci with numerous: after Encarsia formosa honeybee kind indoor growing tobacco reaches 15 days in the step (5), each with numerous room in cultivation on tobacco, tobacco in numerous room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco plant of cultivating in numerous room sprays 800 times of carbendazols, and then tobacco continues cultivation 5 days in numerous room, collect on tobacco plant that Bemisia tabaci adults is inoculated into numerous indoor from aleyrodid worm kind room, every strain tobacco plant inoculates 30 Bemisia tabaci, keep with numerous indoor temperature 24 DEG C, humidity 40%, natural lighting, after 5 days, gather on tobacco plant that cigarette aphid is inoculated into numerous indoor from cigarette aphid aphid kind room, every strain tobacco plant inoculates 20 cigarette aphids, cigarette aphid is breeding with numerous indoor growing together with Bemisia tabaci, once manually plant leaf was shaken to the tobacco with numerous indoor in every 3 days, Bemisia tabaci by even for ovum real estate on the blade with numerous indoor tobacco plant, cigarette aphid and Bemisia tabaci are being 15 days with numerous time controling together with numerous indoor,
(7) aphidius gifuensis together with Encarsia formosa with numerous: cigarette aphid and Bemisia tabaci together with numerous indoor with numerous 15 days after, from Encarsia formosa honeybee kind room, collect Encarsia formosa becomes honeybee to be inoculated into numerous indoor, inoculum concentration compares 1:30 according to Encarsia formosa and Bemisia tabaci worm's ovum, after 4 days, from cocoon honeybee honeybee kind room, collect aphidius gifuensis becomes honeybee to be inoculated into numerous indoor, inoculum concentration compares 1:50 according to aphidius gifuensis and cigarette aphid, keep with numerous indoor temperature 24 DEG C, humidity 40%, fine day 11:00-17:00 time period shading rate be 70% sunshade net carry out shading, all the other time keeps natural lighting condition, after 15 days, the black pupa of a large amount of Encarsia formosas and the stiff aphid of aphidius gifuensis is being obtained with breeding simultaneously on the tobacco plant blade of numerous indoor.
embodiment 2:aphidius gifuensis is with a numerous method together with Encarsia formosa, and the implementation step of the method comprises:
(1) prepare breed condition: be separated out in greenhouse length and width be 4 meters cigarette aphid aphid kind room, aleyrodid worm kind room, cocoon honeybee honeybee kind room, each 1 of Encarsia formosa honeybee kind room, with 10, numerous room, the surrounding of each room above-mentioned all uses the airtight isolation of 80 object fly net;
(2) cigarette aphid aphid kind is bred: in cigarette aphid aphid kind room, cultivate eggplant, eggplant in cigarette aphid aphid kind room conveniently water and fertilizer management natural lighting cultivate 15 days, eggplant plant in cigarette aphid aphid kind room sprays 800 times of carbendazols, and then eggplant continues cultivation 5 days in cigarette aphid aphid kind room; Cigarette aphid is gathered to field, the cigarette aphid collected in field is inoculated on the eggplant plant in cigarette aphid aphid kind room, keep cigarette aphid aphid kind indoor temperature 30 DEG C, humidity 80%, natural lighting, growth and breeding on the eggplant plant of cigarette aphid in cigarette aphid aphid kind room, the cigarette aphid quantity of in cigarette aphid aphid kind room, every strain eggplant plant breeding after 15 days reaches more than 1000;
(3) aphidius gifuensis honeybee kind is bred: after cigarette aphid aphid kind indoor growing eggplant reaches 10 days in step (2), eggplant is cultivated in cocoon honeybee honeybee kind room, eggplant in cocoon honeybee honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, eggplant plant in cocoon honeybee honeybee kind room sprays 800 times of carbendazols, and then eggplant continues cultivation 5 days in cocoon honeybee honeybee kind room, the blade with cigarette aphid is won from the eggplant plant cigarette aphid aphid kind room, the cigarette aphid won on blade is inoculated on the eggplant plant in cocoon honeybee honeybee kind room, every strain eggplant plant inoculates cigarette aphid 15, keep cocoon honeybee honeybee kind indoor temperature 30 DEG C, humidity 80%, natural lighting, growth and breeding on the eggplant plant of cigarette aphid in cocoon honeybee honeybee kind room, the cigarette aphid quantity that after 10 days, in cocoon honeybee honeybee kind room, every strain eggplant plant breeds reaches 150, gather aphidius gifuensis to field and become honeybee, on the eggplant plant that the aphidius gifuensis collected in field becomes honeybee to be inoculated in cocoon honeybee honeybee kind room, inoculative proportion compares 1:100 according to honeybee aphid, cocoon honeybee sex ration 1:2.0, aphidius gifuensis carries out growth reproduction 15 days in cocoon honeybee honeybee kind room, obtain aphidius gifuensis honeybee kind,
(4) aleyrodid worm kind is bred: cigarette aphid aphid kind indoor growing eggplant on the same day in step (2), eggplant seedling is cultivated in aleyrodid worm kind room, eggplant in aleyrodid worm kind room conveniently water and fertilizer management natural lighting cultivate 15 days, cultivate in aleyrodid worm kind room on eggplant plant and spray 800 times of carbendazols, then eggplant continues cultivation 5 days in aleyrodid worm kind room; Bemisia tabaci Nymph is gathered to field, the Bemisia tabaci Nymph collected in field is inoculated on the eggplant plant in aleyrodid worm kind room, keep aleyrodid worm kind indoor temperature 30 DEG C, humidity 80%, natural lighting, growth and breeding on the eggplant plant of Bemisia tabaci Nymph in aleyrodid worm kind room, the Bemisia tabaci quantity of in aleyrodid worm kind room, every strain eggplant plant breeding after 15 days reaches more than 200;
(5) Encarsia formosa honeybee kind is bred: after aleyrodid worm kind indoor growing eggplant reaches 10 days in step (4), eggplant seedling is cultivated in Encarsia formosa honeybee kind room, eggplant in Encarsia formosa honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the eggplant plant cultivated in Encarsia formosa honeybee kind room sprays 800 times of carbendazols, and then eggplant continues cultivation 5 days in Encarsia formosa honeybee kind room, to breed on eggplant plant that the Bemisia tabaci obtained is inoculated in Encarsia formosa honeybee kind room in aleyrodid worm kind room, every strain eggplant plant inoculates Bemisia tabaci 15, keep Encarsia formosa honeybee kind indoor temperature 30 DEG C, humidity 80%, natural lighting, growth and breeding on the eggplant plant of Bemisia tabaci in Encarsia formosa honeybee kind room, the Bemisia tabaci Nymph quantity of in Encarsia formosa honeybee kind room, every strain eggplant plant breeding after 10 days reaches 20, the black pupa of Encarsia formosa is gathered to field, the black pupa of the Encarsia formosa collected in field is inoculated on the eggplant plant in Encarsia formosa honeybee kind room, inoculative proportion compares 1:50 according to honeybee worm, Encarsia formosa carries out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtain Encarsia formosa honeybee kind,
(6) cigarette aphid together with Bemisia tabaci with numerous: after Encarsia formosa honeybee kind indoor growing eggplant reaches 15 days in the step (5), each with numerous room in cultivate eggplant seedling, eggplant in numerous room conveniently water and fertilizer management natural lighting cultivate 15 days, the eggplant plant cultivated in numerous room sprays 800 times of carbendazols, and then eggplant continues cultivation 5 days in numerous room, collect on eggplant plant that Bemisia tabaci Nymph is inoculated into numerous indoor from aleyrodid worm kind room, every strain eggplant plant inoculates 30 Bemisia tabaci, keep with numerous indoor temperature 30 DEG C, humidity 70%, natural lighting, after 5 days, gather on eggplant plant that cigarette aphid is inoculated into numerous indoor from cigarette aphid aphid kind room, every strain eggplant plant inoculates 20 cigarette aphids, cigarette aphid is breeding with numerous indoor growing together with Bemisia tabaci, once manually plant leaf was shaken to the eggplant with numerous indoor in every 5 days, Bemisia tabaci by even for ovum real estate on the blade with numerous indoor eggplant plant, cigarette aphid and Bemisia tabaci are being 15 days with numerous time controling together with numerous indoor,
(7) aphidius gifuensis together with Encarsia formosa with numerous: cigarette aphid and Bemisia tabaci together with numerous indoor with numerous 15 days after, from Encarsia formosa honeybee kind room, collect the black pupa of Encarsia formosa is inoculated into numerous indoor, inoculum concentration compares 1:60 according to Encarsia formosa and Bemisia tabaci worm's ovum, after 4 days, from cocoon honeybee honeybee kind room, collect the stiff aphid of aphidius gifuensis is inoculated into numerous indoor, inoculum concentration compares 1:100 according to aphidius gifuensis and cigarette aphid, keep with numerous indoor temperature 30 DEG C, humidity 80%, fine day 11:00-17:00 time period shading rate be 70% sunshade net carry out shading, all the other time keeps natural lighting condition, after 15 days, the black pupa of a large amount of Encarsia formosas and the stiff aphid of aphidius gifuensis is being obtained with breeding simultaneously on the eggplant plant leaf of numerous indoor.
Claims (1)
1. aphidius gifuensis is with numerous method together with Encarsia formosa, it is characterized in that: the implementation step of the method is:
(1) prepare to breed condition: in greenhouse, be separated out the close cigarette aphid aphid kind room of size, aleyrodid worm kind room, cocoon honeybee honeybee kind room, each 1 of Encarsia formosa honeybee kind room, with numerous room 5-10, the surrounding of each room above-mentioned all uses the airtight isolation of 80 object fly net;
(2) cigarette aphid aphid kind is bred: in cigarette aphid aphid kind room, cultivate tobacco or eggplant seedling, tobacco or eggplant in cigarette aphid aphid kind room conveniently water and fertilizer management natural lighting cultivate 15 days, tobacco in cigarette aphid aphid kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in cigarette aphid aphid kind room; Cigarette aphid is gathered to field, the cigarette aphid collected in field is inoculated on tobacco in cigarette aphid aphid kind room or eggplant plant, keep cigarette aphid aphid kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco of cigarette aphid in cigarette aphid aphid kind room or eggplant plant, the cigarette aphid quantity of in cigarette aphid aphid kind room, every strain tobacco or eggplant plant breeding after 10-15 days reaches more than 1000;
(3) aphidius gifuensis honeybee kind is bred: after cigarette aphid aphid kind indoor growing tobacco or eggplant reach 10 days in step (2), tobacco or eggplant is cultivated in cocoon honeybee honeybee kind room, tobacco or eggplant in cocoon honeybee honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, tobacco in cocoon honeybee honeybee kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in cocoon honeybee honeybee kind room; The blade with cigarette aphid is won from the tobacco cigarette aphid aphid kind room or eggplant plant, the cigarette aphid won on blade is inoculated on tobacco in cocoon honeybee honeybee kind room or eggplant plant, every strain tobacco or eggplant plant inoculate cigarette aphid 15, keep cocoon honeybee honeybee kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco of cigarette aphid in cocoon honeybee honeybee kind room or eggplant plant, the cigarette aphid quantity that after 5-10 days, in cocoon honeybee honeybee kind room, every strain tobacco or eggplant plant breed reaches 100-150 head; The stiff aphid of aphidius gifuensis is gathered or aphidius gifuensis becomes honeybee to field, the stiff aphid of the aphidius gifuensis that collected in field or aphidius gifuensis become honeybee to be inoculated on tobacco in cocoon honeybee honeybee kind room or eggplant plant, inoculative proportion according to honeybee aphid than 1:50-100, cocoon honeybee sex ration 1:0.5-2.0, aphidius gifuensis carries out growth reproduction 15 days in cocoon honeybee honeybee kind room, obtains aphidius gifuensis honeybee kind;
(4) aleyrodid worm kind is bred: cigarette aphid aphid kind indoor growing tobacco or eggplant seedling are on the same day in step (2), tobacco or eggplant seedling on cultivating in aleyrodid worm kind room, tobacco or eggplant seedling in aleyrodid worm kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco cultivated in aleyrodid worm kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant seedling continue cultivation 5 days in aleyrodid worm kind room; Bemisia tabaci or Bemisia tabaci Nymph is gathered to field, the Bemisia tabaci collected in field or Bemisia tabaci Nymph are inoculated on tobacco in aleyrodid worm kind room or eggplant plant, keep aleyrodid worm kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on tobacco in aleyrodid worm kind room of Bemisia tabaci or Bemisia tabaci Nymph or eggplant plant, the Bemisia tabaci quantity of in aleyrodid worm kind room, every strain tobacco or eggplant plant breeding after 10-15 days reaches more than 200;
(5) Encarsia formosa honeybee kind is bred: after aleyrodid worm kind indoor growing tobacco or eggplant seedling reach 10 days in step (4), tobacco or eggplant seedling is cultivated in Encarsia formosa honeybee kind room, tobacco or eggplant in Encarsia formosa honeybee kind room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco cultivated in Encarsia formosa honeybee kind room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in Encarsia formosa honeybee kind room, be inoculated on tobacco in Encarsia formosa honeybee kind room or eggplant plant by breeding the Bemisia tabaci obtained in aleyrodid worm kind room, every strain tobacco or eggplant plant inoculate Bemisia tabaci 15, keep Encarsia formosa honeybee kind indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on the tobacco of Bemisia tabaci in Encarsia formosa honeybee kind room or eggplant plant, the Bemisia tabaci Nymph quantity of in Encarsia formosa honeybee kind room, every strain tobacco or eggplant plant breeding after 10 days reaches 20, Encarsia formosa or the black pupa of Encarsia formosa is gathered to field, the Encarsia formosa collected in field or the black pupa of Encarsia formosa are inoculated on tobacco in Encarsia formosa honeybee kind room or eggplant plant, inoculative proportion compares 1:20-50 according to honeybee worm, Encarsia formosa carries out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtain Encarsia formosa honeybee kind,
(6) cigarette aphid together with Bemisia tabaci with numerous: after Encarsia formosa honeybee kind indoor growing tobacco or eggplant reach 15 days in the step (5), each with numerous room in cultivate tobacco or eggplant seedling, tobacco or eggplant in numerous room conveniently water and fertilizer management natural lighting cultivate 15 days, the tobacco cultivated in numerous room or eggplant plant spray 800 times of carbendazols, and then tobacco or eggplant continue cultivation 5 days in numerous room, from aleyrodid worm kind room, collect Bemisia tabaci adults or Bemisia tabaci Nymph is inoculated into on the tobacco of numerous indoor or eggplant plant, every strain tobacco or eggplant plant inoculate 30 Bemisia tabaci, keep with numerous indoor temperature 24-30 DEG C, humidity 40%-70%, natural lighting, after 5 days, from cigarette aphid aphid kind room, gather cigarette aphid is inoculated into on the tobacco of numerous indoor or eggplant plant, every strain tobacco or eggplant plant inoculate 20 cigarette aphids, cigarette aphid is breeding with numerous indoor growing together with Bemisia tabaci, within every 3-5 days, once manually shake plant leaf to the tobacco of numerous indoor or eggplant, Bemisia tabaci by even for ovum real estate on the blade of tobacco or eggplant plant, cigarette aphid and Bemisia tabaci are being 15 days with numerous time controling together with numerous indoor,
(7) aphidius gifuensis together with Encarsia formosa with numerous: cigarette aphid and Bemisia tabaci together with numerous indoor with numerous 15 days after, from Encarsia formosa honeybee kind room, collect Encarsia formosa becomes honeybee or the black pupa of Encarsia formosa to be inoculated into numerous indoor, inoculum concentration compares 1:30-60 according to Encarsia formosa and Bemisia tabaci worm's ovum, after 4 days, from cocoon honeybee honeybee kind room, collect aphidius gifuensis becomes honeybee or the stiff aphid of aphidius gifuensis to be inoculated into numerous indoor, inoculum concentration compares 1:50-100 according to aphidius gifuensis and cigarette aphid, keep with numerous indoor temperature 24-30 DEG C, humidity 40%-80%, fine day 11:00-17:00 time period shading rate be 70% sunshade net carry out shading, all the other time keeps natural lighting condition, after 15 days, the black pupa of a large amount of Encarsia formosas and the stiff aphid of aphidius gifuensis is being obtained with breeding simultaneously on the tobacco of numerous indoor or eggplant plant leaf.
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