CN104756955B - Aphidius gifuensis are with numerous method together with Encarsia formosa - Google Patents
Aphidius gifuensis are with numerous method together with Encarsia formosa Download PDFInfo
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Abstract
Aphidius gifuensis are with numerous method together with Encarsia formosa, the method is by the use of the poor tobacco of insect resistace or Eggplant Varieties as cigarette aphid and the host plant of Bemisia tabaci, the breeding by classifying to cigarette aphid, aphidius gifuensis, Bemisia tabaci, Encarsia formosa is obtained after corresponding worm kind honeybee kind, cigarette aphid is being carried out with numerous interior and Bemisia tabaci is bred together, then inoculation aphidius gifuensis and Encarsia formosa on numerous room plant, realize aphidius gifuensis and Encarsia formosa with numerous.Greatly improved using the breeding and utilizing rate of host plant of the present invention, can be while numerous obtain 10000 remainder of aphidius gifuensis, 12000 remainder of Encarsia formosa with every plant of host plant in numerous room, 40 60% are improved using the identical facility condition present invention than the efficiency of breeding of commonsense method, the present invention is while numerous obtain two kinds of natural enemy parasitic wasps, the problem that two kinds of insects of cigarette aphid and Bemisia tabaci are caused harm simultaneously in energy effectively solving actual production, prevention effect is good, efficiency high, improves the Prevention Technique to various pests using bio-diversity.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry injurious insect, more particularly to aphidius gifuensis are same together with Encarsia formosa
Numerous method.
Background technology
Cigarette aphid is colonized on the plant of the crops such as Solanaceae, Cruciferae, composite family, pulse family, Chenopodiaceae, Convolvulaceae, the rose family, its
Become, the juice in larvae aphid sucking plant body, make leaf curl, flavescence, stem and bennet distortion, deformity, plant is short and small, is sucking
Transmitted virus disease in journey, excretion honeydew induction tobacco leaf dark mildew.Cigarette aphid endangers larger in agricultural and forestry production.
, used as a kind of worldwide insect, host range is extensive for aleyrodid, it is known that 653 kinds of up to 112 sections, the becoming of aleyrodid
Worm and nymph utilize rasping-sucking mouthparts sucking plant juice, make plant leaf chlorisis, flavescences, wilt, induction and propagation mycosis,
Virosis, the harm on the crops such as flowers, fruit tree, medicinal material, tobacco are extremely serious.
Recently as the development of biological prevention, the continuous research and utilization aphidius gifuensis of people prevent and treat cigarette aphid, using beautiful
Aphid chalcid fly prevents and treats aleyrodid, achieves necessarily in the technical field of the insect parasite controlling disease worm of artificial propagation single variety
Effect；In actual production, two kinds of insects of cigarette aphid and aleyrodid are run into while when endangering to crops, employing and parasitic wasp being entered
The artificial propagation of row single variety, is discharged into field after breeding aphidius gifuensis and Encarsia formosa respectively, parasitic wasp breeds single variety
Prevention Technique cycle length, efficiency are low, operation is many, high cost, cigarette aphid with aleyrodid and meanwhile during damage to crops prevention effect substantially reduce,
The technology that exploration, excavation carry out biological synthesis preventing and treating to various pests is very urgent.
Content of the invention
In agricultural production, for two kinds of insects of cigarette aphid and aleyrodid while during damage to crops, adopting single variety in prior art
Parasitic wasp raising technology breeds aphidius gifuensis and Encarsia formosa respectively, prevents and treats two kinds of insects of cigarette aphid and aleyrodid, there is breeding cycle
Grow, efficiency is low, operation is many, high cost, the problem that prevention effect is substantially reduced, and provides a kind of aphidius gifuensis together with Encarsia formosa
With numerous method, the implementation steps of the method include：
（1）Prepare to breed condition：The close cigarette aphid aphid kind room of size, aleyrodid worm kind room, cocoon honeybee honeybee is separated out in greenhouse
Kind of room, Encarsia formosa honeybee kind room are each 1, and with numerous room 5-10, the fly net of the surrounding of each room above-mentioned all with 80 mesh is closed
Isolation；
（2）Breed cigarette aphid aphid kind：Cultivation tobacco or eggplant seedling in cigarette aphid aphid kind room, tobacco or eggplant seedling are in cigarette aphid aphid kind
Cultivate 15 days according to conventional water and fertilizer management natural lighting in room, on tobacco in cigarette aphid aphid kind room or eggplant seedling plant, spray one
Secondary 800 times of carbendazols, then tobacco or eggplant seedling continue in cigarette aphid aphid kind room to cultivate 5 days；Cigarette aphid is gathered to field, by field
Between the cigarette aphid that collects be inoculated on the tobacco in cigarette aphid aphid kind room or eggplant seedling plant, keep cigarette aphid aphid kind room indoor temperature
24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on tobacco or eggplant seedling plant of the cigarette aphid in cigarette aphid aphid kind room,
The cigarette aphid quantity that breeds on every plant of tobacco or eggplant seedling plant in cigarette aphid aphid kind room after 10-15 days reaches more than 1000；
（3）Breed aphidius gifuensis honeybee kind：Treat that cigarette aphid aphid kind indoor growing tobacco or eggplant seedling reach 10 days in step (2)
Afterwards, cultivation tobacco or eggplant seedling in cocoon honeybee honeybee kind room, tobacco or eggplant seedling are according to conventional water and fertilizer management in cocoon honeybee honeybee kind room
Natural lighting is cultivated 15 days, sprays 800 times of carbendazols, then on tobacco in cocoon honeybee honeybee kind room or eggplant seedling plant
Tobacco or eggplant seedling continue in cocoon honeybee honeybee kind room to cultivate 5 days；Win on tobacco or eggplant seedling plant from cigarette aphid aphid kind room
Blade with cigarette aphid, the cigarette aphid that wins on blade is inoculated on the tobacco in cocoon honeybee honeybee kind room or eggplant seedling plant, per plant
On tobacco or eggplant seedling plant, inoculation cigarette aphid 15, keeps cocoon honeybee honeybee kind room indoor temperature 24-30 DEG C, humidity 30%-80%, nature
Illumination, growth and breeding on tobacco or eggplant seedling plant of the cigarette aphid in cocoon honeybee honeybee kind room, after 5-10 days per plant in cocoon honeybee honeybee kind room
The cigarette aphid quantity that tobacco or eggplant seedling plant breed reaches 100-150 head；Aphidius gifuensis deadlock aphid or aphidius gifuensis are gathered to field
Become honeybee, tobacco or eggplant that the stiff aphid of the aphidius gifuensis that field is collected or aphidius gifuensis become honeybee to be inoculated in cocoon honeybee honeybee kind room
On seedling plant, inoculative proportion compares 1 according to honeybee aphid:50-100, cocoon honeybee sex ration 1:0.5-2.0, aphidius gifuensis are in cocoon honeybee honeybee kind room
In carry out growth reproduction 15 days, obtain aphidius gifuensis honeybee kind；
（4）Breed aleyrodid worm kind：In step (2) cigarette aphid aphid kind indoor growing tobacco or eggplant seedling on the same day, in powder
Upper tobacco or eggplant seedling is cultivated in mite kind room, and tobacco or eggplant seedling are according to conventional water and fertilizer management natural light in aleyrodid worm kind room
According to cultivating 15 days, 800 times of carbendazols, Ran Houyan on the tobacco that cultivates in aleyrodid worm kind room or eggplant seedling plant, is sprayed
Grass or eggplant seedling continue in aleyrodid worm kind room to cultivate 5 days；Bemisia tabaci or Bemisia tabaci Nymph are gathered to field, field is gathered
To Bemisia tabaci or Bemisia tabaci Nymph be inoculated on the tobacco in aleyrodid worm kind room or eggplant seedling plant, keep aleyrodid worm kind room room
Interior temperature 24-30 DEG C, humidity 30%-80%, natural lighting, Bemisia tabaci or Bemisia tabaci Nymph tobacco or eggplant in aleyrodid worm kind room
Growth and breeding on sub- seedling plant, the bemisia tabaci number that breeds on every plant of tobacco or eggplant seedling plant in aleyrodid worm kind room after 10-15 days
Amount reaches more than 200；
（5）Breed Encarsia formosa honeybee kind：After in step (4), aleyrodid worm kind indoor growing tobacco or eggplant seedling reach 10 days,
Cultivation tobacco or eggplant seedling in Encarsia formosa honeybee kind room, tobacco or eggplant seedling are according to conventional liquid manure in Encarsia formosa honeybee kind room
Management natural lighting cultivate 15 days, in Encarsia formosa honeybee kind room cultivate tobacco or eggplant seedling plant on sprinkling one time 800 times
Carbendazol, then tobacco or eggplant seedling continue in Encarsia formosa honeybee kind room to cultivate 5 days；Obtain breeding in aleyrodid worm kind room
Bemisia tabaci be inoculated on the tobacco in Encarsia formosa honeybee kind room or eggplant seedling plant, on every plant of tobacco or eggplant seedling plant be inoculated with
Bemisia tabaci 15, keeps Encarsia formosa honeybee kind room indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, and Bemisia tabaci is beautiful
Growth and breeding on tobacco or eggplant seedling plant in aphid chalcid fly honeybee kind room, every plant of tobacco or eggplant in Encarsia formosa honeybee kind room after 10 days
The Bemisia tabaci Nymph quantity that breeds on sub- seedling plant reaches 20, gathers Encarsia formosa or the black pupa of Encarsia formosa to field, by field
Between the Encarsia formosa that collects or the black pupa of Encarsia formosa be inoculated on the tobacco in Encarsia formosa honeybee kind room or eggplant seedling plant,
Inoculative proportion compares 1 according to honeybee worm:20-50, Encarsia formosa carry out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtain beautiful aphid
Chalcid fly honeybee kind；
（6）Cigarette aphid and Bemisia tabaci are together with numerous：Treat that Encarsia formosa honeybee kind indoor growing tobacco or eggplant seedling reach in step (5)
After 15 days, tobacco or eggplant seedling is cultivated in each is with numerous room, tobacco or eggplant seedling are according to conventional water and fertilizer management in numerous room
Natural lighting is cultivated 15 days, sprays 800 times of carbendazols, then in numerous room on the tobacco that cultivates or eggplant seedling plant
Tobacco or eggplant seedling continue in numerous room to cultivate 5 days；Bemisia tabaci adults are collected from aleyrodid worm kind room or Bemisia tabaci Nymph connects
Plant with numerous indoor tobacco or eggplant seedling plant, on every plant of tobacco or eggplant seedling plant, 30 Bemisia tabaci are inoculated with, keep same
Numerous room indoor temperature 24-30 DEG C, humidity 40%-70%, natural lighting, after 5 days, from cigarette aphid aphid kind room, collection cigarette aphid is inoculated into
With on numerous indoor tobacco or eggplant seedling plant, 20 cigarette aphids, cigarette aphid and Bemisia tabaci on every plant of tobacco or eggplant seedling plant, is inoculated with
Breeding with numerous indoor growing together, per 3-5 days to once manually being shaken Plant Leaf with numerous indoor tobacco or eggplant seedling
Piece, by uniform for ovum real estate on the blade of tobacco or eggplant seedling plant, cigarette aphid and Bemisia tabaci are with numerous indoor same together for Bemisia tabaci
Numerous time control is 15 days；
（7）Aphidius gifuensis and Encarsia formosa are together with numerous：Cigarette aphid and Bemisia tabaci after with numerous interior together with numerous 15 days, from
Collecting Encarsia formosa in Encarsia formosa honeybee kind room becomes honeybee or the black pupa of Encarsia formosa to be inoculated into numerous interior, and inoculum concentration is little according to beautiful aphid
Honeybee compares 1 with Bemisia tabaci worm's ovum:30-60, after 4 days, collects aphidius gifuensis from cocoon honeybee honeybee kind room and becomes honeybee or aphidius gifuensis deadlock aphid to connect
Plant and arrive with numerous interior, inoculum concentration compares 1 according to aphidius gifuensis with cigarette aphid:50-100, keeps with numerous room indoor temperature 24-30 DEG C, wet
Degree 40%-80%, fine day 11:00-17:00 time period carried out shading with the sunshade net that shading rate is 70%, and remaining time keeps nature
Illumination condition, after 15 days, while breeding obtains substantial amounts of Encarsia formosa on numerous indoor tobacco or eggplant seedling plant leaf
Black pupa and aphidius gifuensis deadlock aphid；
The present invention by the use of insect resistace difference tobacco or Eggplant Varieties as cigarette aphid and the host plant of Bemisia tabaci, by cigarette
Aphid, aphidius gifuensis, Bemisia tabaci, Encarsia formosa carry out classifying after breeding obtains corresponding worm kind honeybee kind, are carrying out with numerous interior
Cigarette aphid and Bemisia tabaci are cooperatively bred, and then inoculation aphidius gifuensis and Encarsia formosa on numerous room plant, realize cigarette aphid cocoon
Honeybee and Encarsia formosa are greatly improved using the breeding and utilizing rate of host plant of the present invention with numerous, aphidius gifuensis and Encarsia formosa
Breeding effect is good, can be while numerous obtain 10000 remainder of aphidius gifuensis, Encarsia formosa more than 12000 with every plant of host plant in numerous room
Head, the efficiency of breeding for breeding efficiency than carrying out single variety respectively of the identical facility condition present invention improve 40-60%, the present invention
Two kind natural enemy parasitic wasps can be obtained simultaneously numerous, two kinds of insects of cigarette aphid and Bemisia tabaci in energy effectively solving actual production are while caused harm asks
Topic, prevention effect is good, efficiency high, can make full use of biological prevention of the bio-diversity lifting to various pests.
Specific embodiment
Embodiment 1：With numerous method together with Encarsia formosa, the implementation steps of the method include a kind of aphidius gifuensis：
（1）Prepare to breed condition：Be separated out in greenhouse length and width be 4 meters cigarette aphid aphid kind room, aleyrodid worm kind room, cocoon
Honeybee honeybee kind room, Encarsia formosa honeybee kind room are each 1, and with numerous room 5, the fly net of the surrounding of each room above-mentioned all with 80 mesh is closed
Isolation；
（2）Breed cigarette aphid aphid kind：Tobacco is cultivated in cigarette aphid aphid kind room, tobacco is according to conventional liquid manure in cigarette aphid aphid kind room
Management natural lighting is cultivated 15 days, sprays 800 times of carbendazols, then tobacco on the tobacco plant in cigarette aphid aphid kind room
Continue in cigarette aphid aphid kind room to cultivate 5 days；Cigarette aphid is gathered to field, the cigarette aphid that field is collected is inoculated into cigarette aphid aphid kind room
In tobacco plant on, keep cigarette aphid aphid kind room indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, cigarette aphid is in cigarette aphid
Growth and breeding on tobacco plant in aphid kind room, the cigarette aphid quantity that breeds on every plant of tobacco plant in cigarette aphid aphid kind room after 10 days reach
To more than 1000；
（3）Breed aphidius gifuensis honeybee kind：After in step (2), cigarette aphid aphid kind indoor growing tobacco reaches 10 days, in cocoon honeybee
Tobacco is cultivated in honeybee kind room, and tobacco is cultivated 15 days according to conventional water and fertilizer management natural lighting in cocoon honeybee honeybee kind room, in cocoon honeybee honeybee
Plant on the tobacco plant in room and 800 times of carbendazols are sprayed, then tobacco continues in cocoon honeybee honeybee kind room to cultivate 5 days；From
The blade with cigarette aphid is won on tobacco plant in cigarette aphid aphid kind room, and the cigarette aphid that wins on blade is inoculated into cocoon honeybee honeybee kind room
In tobacco plant on, on every plant of tobacco plant, inoculation cigarette aphid 15, keeps 24 DEG C of cocoon honeybee honeybee kind room indoor temperature, humidity
30%%th, natural lighting, growth and breeding on tobacco plant of the cigarette aphid in cocoon honeybee honeybee kind room, every plant of cigarette in cocoon honeybee honeybee kind room after 5 days
The cigarette aphid quantity that breeds on careless plant reaches 100, gathers aphidius gifuensis deadlock aphid, the cigarette aphid cocoon that field is collected to field
Deadlock aphid is inoculated on the tobacco plant in cocoon honeybee honeybee kind room honeybee, and inoculative proportion compares 1 according to honeybee aphid:50th, cocoon honeybee sex ration 1:0.5,
Aphidius gifuensis carry out growth reproduction 15 days in cocoon honeybee honeybee kind room, obtain aphidius gifuensis honeybee kind；
（4）Breed aleyrodid worm kind：In step (2) cigarette aphid aphid kind indoor growing tobacco on the same day, in aleyrodid worm kind room
Middle cultivation tobacco, tobacco are cultivated 15 days according to conventional water and fertilizer management natural lighting in aleyrodid worm kind room, in aleyrodid worm kind room
800 times of carbendazols are sprayed on the tobacco plant of cultivation, and then tobacco continues in aleyrodid worm kind room to cultivate 5 days；To field
Between gather Bemisia tabaci, the Bemisia tabaci that field is collected is inoculated on the tobacco plant in aleyrodid worm kind room, keeps aleyrodid worm
24 DEG C of kind room indoor temperature, humidity 30%, natural lighting, growth and breeding on tobacco plant of the Bemisia tabaci in aleyrodid worm kind room, 10
The Bemisia tabaci quantity that breeds on every plant of tobacco plant in aleyrodid worm kind room after it reaches more than 200；
（5）Breed Encarsia formosa honeybee kind：After in step (4), aleyrodid worm kind indoor growing tobacco reaches 10 days, in beautiful aphid
Tobacco is cultivated in chalcid fly honeybee kind room, and tobacco is cultivated 15 days according to conventional water and fertilizer management natural lighting in Encarsia formosa honeybee kind room,
800 times of carbendazols are sprayed on the tobacco plant that cultivates in Encarsia formosa honeybee kind room, then tobacco is in Encarsia formosa honeybee
Plant in room and continue to cultivate 5 days；The Bemisia tabaci that obtains will be bred in aleyrodid worm kind room is inoculated into the tobacco in Encarsia formosa honeybee kind room
On plant, on every plant of tobacco plant, be inoculated with Bemisia tabaci 15, keep 24 DEG C of Encarsia formosa honeybee kind room indoor temperature, humidity 30%, from
So illumination, growth and breeding on tobacco plant of the Bemisia tabaci in Encarsia formosa honeybee kind room, every in Encarsia formosa honeybee kind room after 10 days
The Bemisia tabaci Nymph quantity that breeds on strain tobacco plant reaches 20, gathers Encarsia formosa to field, field is collected
Encarsia formosa is inoculated on the tobacco plant in Encarsia formosa honeybee kind room, and inoculative proportion compares 1 according to honeybee worm:20, Encarsia formosa exists
Encarsia formosa honeybee kind carries out growth reproduction 15 days in room, obtains Encarsia formosa honeybee kind；
（6）Cigarette aphid and Bemisia tabaci are together with numerous：Treat that Encarsia formosa honeybee kind indoor growing tobacco reaches 15 days in step (5)
Afterwards, tobacco is cultivated in each is with numerous room, and tobacco is cultivated 15 days according to conventional water and fertilizer management natural lighting in numerous room,
With 800 times of carbendazols are sprayed on the tobacco plant that cultivates in numerous room, then tobacco continues in numerous room to cultivate 5 days；
Bemisia tabaci adults are collected from aleyrodid worm kind room be inoculated into on numerous indoor tobacco plant, be inoculated with 30 on every plant of tobacco plant
Bemisia tabaci, keeps with 24 DEG C of numerous room indoor temperature, humidity 40%, natural lighting, after 5 days, gathers cigarette aphid from cigarette aphid aphid kind room
It is inoculated into on numerous indoor tobacco plant, 20 cigarette aphids on every plant of tobacco plant, is inoculated with, cigarette aphid and Bemisia tabaci is together with numerous
Indoor growing is bred, and per 3 days to once manually being shaken plant leaf with numerous indoor tobacco, Bemisia tabaci is by uniform for ovum real estate
On the blade with numerous interior tobacco plant, cigarette aphid and Bemisia tabaci with numerous indoor be 15 days with numerous time control together；
（7）Aphidius gifuensis and Encarsia formosa are together with numerous：Cigarette aphid and Bemisia tabaci after with numerous interior together with numerous 15 days, from
Collecting Encarsia formosa in Encarsia formosa honeybee kind room becomes honeybee to be inoculated into numerous interior, and inoculum concentration is according to Encarsia formosa and Bemisia tabaci worm's ovum
Than 1:After 30,4 days, aphidius gifuensis being collected from cocoon honeybee honeybee kind room and becoming honeybee to be inoculated into numerous interior, inoculum concentration is according to aphidius gifuensis
Compare 1 with cigarette aphid:50, keep with 24 DEG C of numerous room indoor temperature, humidity 40%, fine day 11:00-17:00 time period shading rate is
70% sunshade net carries out shading, and remaining time keeps natural lighting condition, after 15 days, with numerous indoor tobacco plant blade
Upper breeding simultaneously obtains the black pupa of substantial amounts of Encarsia formosa and aphidius gifuensis deadlock aphid.
Embodiment 2：With numerous method together with Encarsia formosa, the implementation steps of the method include a kind of aphidius gifuensis：
（1）Prepare to breed condition：Be separated out in greenhouse length and width be 4 meters cigarette aphid aphid kind room, aleyrodid worm kind room, cocoon
Honeybee honeybee kind room, Encarsia formosa honeybee kind room are each 1, and with numerous room 10, the fly net of the surrounding of each room above-mentioned all with 80 mesh is close
Close isolation；
（2）Breed cigarette aphid aphid kind：Eggplant seedling is cultivated in cigarette aphid aphid kind room, eggplant seedling is according to routine in cigarette aphid aphid kind room
Water and fertilizer management natural lighting is cultivated 15 days, sprays 800 times of carbendazols, so on the eggplant seedling plant in cigarette aphid aphid kind room
Eggplant seedling continues in cigarette aphid aphid kind room to cultivate 5 days afterwards；Cigarette aphid is gathered to field, the cigarette aphid that field is collected is inoculated into cigarette
On eggplant seedling plant in aphid aphid kind room, 30 DEG C of cigarette aphid aphid kind room indoor temperature, humidity 80%, natural lighting is kept, cigarette aphid is in cigarette
Growth and breeding on eggplant seedling plant in aphid aphid kind room, the cigarette aphid bred on every plant of eggplant seedling plant in cigarette aphid aphid kind room after 15 days
Quantity reaches more than 1000；
（3）Breed aphidius gifuensis honeybee kind：After in step (2), cigarette aphid aphid kind indoor growing reaches 10 days, in cocoon honeybee honeybee kind
Eggplant seedling is cultivated in room, and eggplant seedling is cultivated 15 days according to conventional water and fertilizer management natural lighting in cocoon honeybee honeybee kind room, in cocoon honeybee honeybee
Plant on the eggplant seedling plant in room and 800 times of carbendazols are sprayed, then eggplant seedling continues in cocoon honeybee honeybee kind room to cultivate 5
My god；The blade with cigarette aphid is won on eggplant seedling plant from cigarette aphid aphid kind room, and the cigarette aphid that wins on blade is inoculated into cocoon
On eggplant seedling plant in honeybee honeybee kind room, on every plant of eggplant seedling plant, inoculation cigarette aphid 15, keeps cocoon honeybee honeybee kind room indoor temperature
30 DEG C, humidity 80%, natural lighting, growth and breeding on eggplant seedling plant of the cigarette aphid in cocoon honeybee honeybee kind room, cocoon honeybee honeybee kind after 10 days
The cigarette aphid quantity that every plant of eggplant seedling plant breeds in room reaches 150, gathers aphidius gifuensis to field and becomes honeybee, field is gathered
To aphidius gifuensis become on the eggplant seedling plant that honeybee is inoculated in cocoon honeybee honeybee kind room, inoculative proportion compares 1 according to honeybee aphid:100th, cocoon honeybee
Sex ration 1:2.0, aphidius gifuensis carry out growth reproduction 15 days in cocoon honeybee honeybee kind room, obtain aphidius gifuensis honeybee kind；
（4）Breed aleyrodid worm kind：In step (2) cigarette aphid aphid kind indoor growing eggplant seedling on the same day, in aleyrodid worm kind
Eggplant seedling is cultivated in room, and eggplant seedling is cultivated 15 days according to conventional water and fertilizer management natural lighting in aleyrodid worm kind room, in aleyrodid worm
Plant in room to cultivate on eggplant seedling plant and 800 times of carbendazols are sprayed, then eggplant seedling continues in aleyrodid worm kind room to cultivate 5
My god；Bemisia tabaci Nymph is gathered to field, the Bemisia tabaci Nymph that field is collected is inoculated into the eggplant seedling in aleyrodid worm kind room
On plant, 30 DEG C of aleyrodid worm kind room indoor temperature, humidity 80%, natural lighting is kept, Bemisia tabaci Nymph is in aleyrodid worm kind room
Growth and breeding on eggplant seedling plant, the Bemisia tabaci quantity that breeds on every plant of eggplant seedling plant in aleyrodid worm kind room after 15 days reach
More than 200；
（5）Breed Encarsia formosa honeybee kind：After in step (4), aleyrodid worm kind indoor growing eggplant seedling reaches 10 days, in beautiful aphid
Eggplant seedling is cultivated in chalcid fly honeybee kind room, and eggplant seedling cultivates 15 according to conventional water and fertilizer management natural lighting in Encarsia formosa honeybee kind room
My god, 800 times of carbendazols are sprayed on the eggplant seedling plant that cultivates in Encarsia formosa honeybee kind room, then eggplant seedling is in beautiful aphid
Continue to cultivate 5 days in chalcid fly honeybee kind room；The Bemisia tabaci that obtains will be bred be inoculated in Encarsia formosa honeybee kind room in aleyrodid worm kind room
Eggplant seedling plant on, be inoculated with Bemisia tabaci 15 on every plant of eggplant seedling plant, keep 30 DEG C of Encarsia formosa honeybee kind room indoor temperature,
Humidity 80%, natural lighting, growth and breeding on eggplant seedling plant of the Bemisia tabaci in Encarsia formosa honeybee kind room, after 10 days, beautiful aphid is little
The Bemisia tabaci Nymph quantity that breeds on every plant of eggplant seedling plant in honeybee honeybee kind room reaches 20, gathers Encarsia formosa to field black
Pupa, the black pupa of the Encarsia formosa that field is collected are inoculated on the eggplant seedling plant in Encarsia formosa honeybee kind room, inoculative proportion
Compare 1 according to honeybee worm:50, Encarsia formosa carries out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtains Encarsia formosa honeybee kind；
（6）Cigarette aphid and Bemisia tabaci are together with numerous：Treat that Encarsia formosa honeybee kind indoor growing eggplant seedling reaches 15 days in step (5)
Afterwards, eggplant seedling is cultivated in each is with numerous room, and eggplant seedling is cultivated 15 days according to conventional water and fertilizer management natural lighting in numerous room,
800 times of carbendazols are sprayed on the eggplant seedling plant that cultivates in numerous room, and then eggplant seedling continues training in numerous room
Educate 5 days；Collect Bemisia tabaci Nymph from aleyrodid worm kind room to be inoculated on the eggplant seedling plant with numerous interior, every plant of eggplant seedling plant
30 Bemisia tabaci of upper inoculation, keep with 30 DEG C of numerous room indoor temperature, humidity 70%, natural lighting, after 5 days, from cigarette aphid aphid kind room
Middle collection cigarette aphid is inoculated into on numerous indoor eggplant seedling plant, is inoculated with 20 cigarette aphids, cigarette aphid and cigarette on every plant of eggplant seedling plant
Aleyrodid is being bred with numerous indoor growing together, per 5 days to once manually being shaken plant leaf, cigarette with numerous indoor eggplant seedling
, by uniform for ovum real estate on the blade with numerous interior eggplant seedling plant, cigarette aphid and Bemisia tabaci are with numerous interior together with numerous for aleyrodid
Time control is 15 days；
（7）Aphidius gifuensis and Encarsia formosa are together with numerous：Cigarette aphid and Bemisia tabaci after with numerous interior together with numerous 15 days, from
Collect the black pupa of Encarsia formosa to be inoculated into numerous interior in Encarsia formosa honeybee kind room, inoculum concentration is according to Encarsia formosa and Bemisia tabaci worm's ovum
Than 1:After 60,4 days, aphidius gifuensis deadlock aphid being collected from cocoon honeybee honeybee kind room and being inoculated into numerous interior, inoculum concentration is according to aphidius gifuensis
Compare 1 with cigarette aphid:100, keep with 30 DEG C of numerous room indoor temperature, humidity 80%, fine day 11:00-17:00 time period shading rate is
70% sunshade net carries out shading, and remaining time keeps natural lighting condition, after 15 days, with numerous indoor eggplant seedling Plant Leaf
While breeding obtains the black pupa of substantial amounts of Encarsia formosa and aphidius gifuensis deadlock aphid on piece.
Claims (1)
1. aphidius gifuensis are with numerous method together with Encarsia formosa, it is characterised in that：The implementation steps of the method are：
（1）Prepare to breed condition：Be separated out in greenhouse the close cigarette aphid aphid kind room of size, aleyrodid worm kind room, cocoon honeybee honeybee kind room,
Encarsia formosa honeybee kind room is each 1, and with numerous room 5-10, the surrounding of each room above-mentioned is all with the closed isolation of the fly net of 80 mesh；
（2）Breed cigarette aphid aphid kind：Cultivation tobacco or eggplant seedling in cigarette aphid aphid kind room, tobacco or eggplant seedling are in cigarette aphid aphid kind room
Cultivate 15 days according to conventional water and fertilizer management natural lighting, spray once on tobacco in cigarette aphid aphid kind room or eggplant seedling plant
800 times of carbendazols, then tobacco or eggplant seedling continue in cigarette aphid aphid kind room to cultivate 5 days；Cigarette aphid is gathered to field, by field
The cigarette aphid for collecting is inoculated on the tobacco in cigarette aphid aphid kind room or eggplant seedling plant, keeps cigarette aphid aphid kind room indoor temperature
24-30 DEG C, humidity 30%-80%, natural lighting, growth and breeding on tobacco or eggplant seedling plant of the cigarette aphid in cigarette aphid aphid kind room,
The cigarette aphid quantity that breeds on every plant of tobacco or eggplant seedling plant in cigarette aphid aphid kind room after 10-15 days reaches more than 1000；
（3）Breed aphidius gifuensis honeybee kind：After in step (2), cigarette aphid aphid kind indoor growing tobacco or eggplant seedling reach 10 days,
Cultivation tobacco or eggplant seedling in cocoon honeybee honeybee kind room, tobacco or eggplant seedling are according to conventional water and fertilizer management natural light in cocoon honeybee honeybee kind room
According to cultivating 15 days, on tobacco in cocoon honeybee honeybee kind room or eggplant seedling plant, spray 800 times of carbendazols, then tobacco or
Eggplant seedling continues in cocoon honeybee honeybee kind room to cultivate 5 days；Win with cigarette on tobacco or eggplant seedling plant from cigarette aphid aphid kind room
The blade of aphid, the cigarette aphid that wins on blade is inoculated on the tobacco in cocoon honeybee honeybee kind room or eggplant seedling plant, every plant of tobacco or
On eggplant seedling plant, inoculation cigarette aphid 15, keeps cocoon honeybee honeybee kind room indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting,
Growth and breeding on tobacco or eggplant seedling plant of the cigarette aphid in cocoon honeybee honeybee kind room, after 5-10 days in cocoon honeybee honeybee kind room every plant of tobacco or
The cigarette aphid quantity that eggplant seedling plant breeds reaches 100-150 head；Aphidius gifuensis deadlock aphid is gathered to field or aphidius gifuensis become honeybee, will
Aphidius gifuensis that field collects deadlock aphid or aphidius gifuensis become the tobacco or eggplant seedling plant that honeybee is inoculated in cocoon honeybee honeybee kind room
On, inoculative proportion compares 1 according to honeybee aphid:50-100, cocoon honeybee sex ration 1:0.5-2.0, aphidius gifuensis are carried out in cocoon honeybee honeybee kind room
Growth reproduction 15 days, obtains aphidius gifuensis honeybee kind；
（4）Breed aleyrodid worm kind：In step (2) cigarette aphid aphid kind indoor growing tobacco or eggplant seedling on the same day, in aleyrodid worm
The upper tobacco of cultivation in room or eggplant seedling is planted, tobacco or eggplant seedling are trained according to conventional water and fertilizer management natural lighting in aleyrodid worm kind room
Educate 15 days, in aleyrodid worm kind room cultivate tobacco or eggplant seedling plant on spray 800 times of carbendazols, then tobacco or
Eggplant seedling continues in aleyrodid worm kind room to cultivate 5 days；Bemisia tabaci or Bemisia tabaci Nymph are gathered to field, field is collected
Bemisia tabaci or Bemisia tabaci Nymph are inoculated on the tobacco in aleyrodid worm kind room or eggplant seedling plant, keep aleyrodid worm kind room Indoor Temperature
24-30 DEG C of degree, humidity 30%-80%, natural lighting, Bemisia tabaci or Bemisia tabaci Nymph tobacco or eggplant seedling in aleyrodid worm kind room
Growth and breeding on plant, the Bemisia tabaci quantity that breeds on every plant of tobacco or eggplant seedling plant in aleyrodid worm kind room after 10-15 days reach
To more than 200；
（5）Breed Encarsia formosa honeybee kind：After in step (4), aleyrodid worm kind indoor growing tobacco or eggplant seedling reach 10 days, beautiful
Cultivation tobacco or eggplant seedling in aphid chalcid fly honeybee kind room, tobacco or eggplant seedling are according to conventional water and fertilizer management in Encarsia formosa honeybee kind room
Natural lighting is cultivated 15 days, sprays a bacterium more than 800 times in Encarsia formosa honeybee kind room on the tobacco that cultivates or eggplant seedling plant
Ichor, then tobacco or eggplant seedling continue in Encarsia formosa honeybee kind room to cultivate 5 days；The cigarette for obtaining will be bred in aleyrodid worm kind room
Aleyrodid is inoculated on the tobacco in Encarsia formosa honeybee kind room or eggplant seedling plant, is inoculated with tobacco powder on every plant of tobacco or eggplant seedling plant
Lice 15, keeps Encarsia formosa honeybee kind room indoor temperature 24-30 DEG C, humidity 30%-80%, natural lighting, and Bemisia tabaci is little in beautiful aphid
Growth and breeding on tobacco in honeybee honeybee kind room or eggplant seedling plant, every plant of tobacco or eggplant seedling in Encarsia formosa honeybee kind room after 10 days
The Bemisia tabaci Nymph quantity that breeds on plant reaches 20, gathers Encarsia formosa or the black pupa of Encarsia formosa to field, field is adopted
The Encarsia formosa that collection is obtained or the black pupa of Encarsia formosa are inoculated on the tobacco in Encarsia formosa honeybee kind room or eggplant seedling plant, inoculation
Ratio compares 1 according to honeybee worm:20-50, Encarsia formosa carry out growth reproduction 15 days in Encarsia formosa honeybee kind room, obtain Encarsia formosa
Honeybee kind；
（6）Cigarette aphid and Bemisia tabaci are together with numerous：Treat that Encarsia formosa honeybee kind indoor growing tobacco or eggplant seedling reach 15 days in step (5)
Afterwards, cultivation tobacco or eggplant seedling in each is with numerous room, tobacco or eggplant seedling are according to conventional water and fertilizer management nature in numerous room
Illumination is cultivated 15 days, sprays 800 times of carbendazols, then tobacco in numerous room on the tobacco that cultivates or eggplant seedling plant
Or eggplant seedling continues in numerous room to cultivate 5 days；Bemisia tabaci adults are collected from aleyrodid worm kind room or Bemisia tabaci Nymph is inoculated into
With on numerous indoor tobacco or eggplant seedling plant, on every plant of tobacco or eggplant seedling plant, 30 Bemisia tabaci are inoculated with, keep with numerous room
Indoor temperature 24-30 DEG C, humidity 40%-70%, natural lighting, after 5 days, from cigarette aphid aphid kind room, collection cigarette aphid is inoculated into numerous
On indoor tobacco or eggplant seedling plant, 20 cigarette aphids on every plant of tobacco or eggplant seedling plant, are inoculated with, cigarette aphid and Bemisia tabaci are together
Breeding with numerous indoor growing, per 3-5 days to once manually being shaken plant leaf, cigarette with numerous indoor tobacco or eggplant seedling
Aleyrodid by uniform for ovum real estate on the blade of tobacco or eggplant seedling plant, cigarette aphid and Bemisia tabaci with numerous indoor together with numerous when
Between be controlled to 15 days；
（7）Aphidius gifuensis and Encarsia formosa are together with numerous：Cigarette aphid and Bemisia tabaci after with numerous interior together with numerous 15 days, from beautiful aphid
Collecting Encarsia formosa in chalcid fly honeybee kind room becomes honeybee or the black pupa of Encarsia formosa to be inoculated into numerous interior, inoculum concentration according to Encarsia formosa with
Bemisia tabaci worm's ovum compares 1:30-60, after 4 days, collects aphidius gifuensis from cocoon honeybee honeybee kind room and becomes honeybee or aphidius gifuensis deadlock aphid to be inoculated into
With numerous interior, inoculum concentration compares 1 according to aphidius gifuensis with cigarette aphid:50-100, keeps with numerous room indoor temperature 24-30 DEG C, humidity
40%-80%, fine day 11:00-17:00 time period carried out shading with the sunshade net that shading rate is 70%, and remaining time keeps natural light
According to condition, after 15 days, black substantial amounts of Encarsia formosa is obtained with breeding simultaneously on numerous indoor tobacco or eggplant seedling plant leaf
Pupa and aphidius gifuensis deadlock aphid.
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| CN105145490B (en) * | 2015-08-21 | 2018-10-12 | 山东临沂烟草有限公司 | A kind of method and its application of vegetable seedling breeding aphidius gifuensis |
| CN114467580A (en) * | 2022-01-12 | 2022-05-13 | 山东潍坊烟草有限公司 | Insect prevention method for tobacco leaves |
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