CN105075576B - The method of organic plantation maca - Google Patents
The method of organic plantation maca Download PDFInfo
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- CN105075576B CN105075576B CN201510237753.9A CN201510237753A CN105075576B CN 105075576 B CN105075576 B CN 105075576B CN 201510237753 A CN201510237753 A CN 201510237753A CN 105075576 B CN105075576 B CN 105075576B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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Abstract
The invention discloses a kind of method of organic plantation maca, comprises the following steps a, prepares seedling nutritious soil, b, seed treatment, c, sow, d, kind period management, e, seedling period management, f, seedling, g, hardening, using microbial bacterial agent of the present invention, and the method using organic plantation maca provided by the invention, healthy and strong, disease-free maca seedling can be cultivated, is embodied in, seed germination rate is high, and survival rate is high, and seedling is healthy and strong, growing way is good after culture transferring, and yield is high, no disease and pests harm.
Description
Technical field
The invention belongs to agricultural technology field, and in particular to a kind of method of organic plantation maca.
Background technology
Maca (MACA) is to originate in a kind of crucifer of South America.Leaf is oval, rhizome likeness in form turnip radish, can
It is edible, it is a kind of natural food, nutrition is enriched, and has the reputation of " South America ginseng ".Suitably in High aititude, low latitudes, high daytime
Grown in the night temperature difference, subacidity sandy loam, sun-drenched soil.
Organic maca is suitably planted in high and cold mountain area, Yunnan Province of China province northwest, the height that northeast height above sea level is 2800-3500 rice
Cold is cool, day and night temperature is big, region with abundant light supply is maca Suitable planting area.5-12 DEG C of average temperature of the whole year typically is required, it is raw
Long-term 10-20 DEG C of day and night temperature, 7-9 month average maximum air temperatures are no more than 18 DEG C；Average annual precipitation should be empty in 800-1000mm
Gas relative humidity is in 60-80%, 7-9 month soil moisture contents 20-30%.Plantation plot should be opposed flattened, draining is good, soil
The sandy loam that earth is fertile, the soil is porous.The general pest and disease damage of maca has powdery mildew, especially aphid, attention control subterranean pest-insect.
Organic agriculture refers to the biology in accordance with specific agricultural production principle, not using genetic engineering to obtain in production
And its product, without using materials such as the agricultural chemicals of chemical synthesis, chemical fertilizer, growth regulator, feed addictives, it then follows the natural law and
Ecological principle, coordinate the balance of planting industry and aquaculture, use the agricultural technologys of sustainable developments a series of to maintain to continue
A kind of mode of agriculture of stable system agricultural industry.
Microbial bacterial agent refers to that one kind makes full use of human and animal excreta, agricultural wastes etc. to add biological bacterium fermentation to form,
Using secondary fermentation.But microbial bacterial agent at present on the market is difficult to have the effect sterilized with deinsectization, people simultaneously
Inevitably need to use medicament again while using microbial bacterial agent when maca is planted, thus run counter to green
The organic original intention of color.
Maca is named as rareness species by international genetic research tissue, and only 400 tons of annual production at present is (according to for each person every day
3-5 grams of dosage, it is edible 1 year that current annual 400 tons yield is only sufficient to 300,000 people), in the U.S., Germany, Japan, Southeast Asia etc.
Area is in great demand the several years, and for global ten big situation of selling well health products, at present, all kinds of health products produced using maca as primary raw material are in Europe
The state such as U.S., Japan in the market is promoted rapidly, obtains the favor of more and more consumers, supply falls short of demand.How to search out one kind can
There is good commercial promise, the method for organic plantation maca to cultivate the organic implantation methods for building strong, disease-free maca
Also increasing contribution will be made for the development of the healthy cause of the mankind.
The content of the invention
Present invention seek to address that one of technical problem present in prior art, therefore, it is an object of the present invention to
Provide a kind of organic plantation maca technology, it is intended to cultivate healthy and strong, disease-free maca seedling.
It should be noted that the present invention is the following discovery based on inventor and completed：
According to an aspect of the present invention, the invention provides a kind of method of organic plantation maca, comprise the following steps：
A, seedling nutritious soil is prepared：(it is by perlite, turf, decomposed fertilizer, black earth, grass by microbial bacterial agent and nursery soil
Wood ash, calcium superphosphate etc. form) mixed after it is standby；
B, seed treatment：The maca seed that selection is dry, bright, plumpness is good.Sieving, with 40 degrees Celsius of warm water
Filtering, is soaked with 200 times of water of 100g microbial bacterias dilution agent after filtering, pulls seed out after 20 minutes, wipe kind of a sublist
Face moisture content, be placed on ventilating and cooling place dry it is standby.
C, sow：Seedling nutritious soil is fitted into seedlings nursing plate, be floating, slight compacting；Watering, makes Nutrition Soil in whole seed plate
All moistenings；Seed is uniformly dialled in disk afterwards, carries out blinding；Water again, blinding is moistened completely, seed plate is placed on
In seedling raising greenhouse, into germination period management.
D, period management is planted：Skylight, side window are after planting closed, regulation greenhouse humidity is 65~70%, and regulation and control room temperature on daytime is
26~30 degrees Celsius, night room temperature is 8~10 degrees Celsius, closes sunshade net；Sooner or later ventilation half an hour and watering 15 minutes daily；
Start seedling after 4~5 days；It is surprisingly found by the inventors that the control of humidity and room temperature is very heavy for the effect of the present invention
Want, implementing technical scheme under humidity and temperature conditionss outside the above range will be difficult to the present invention's
Technique effect.
E, seedling period management：After maca goes out seedling in the step d, regulation greenhouse humidity is 65%~70%, and regulation and control are white
Its room temperature is 26~30 degrees Celsius, and night room temperature is 10~12 degrees Celsius, and sunshade net opens 30~35cm, and ventilation 1~3 is small daily
When, watering 10~15 minutes sooner or later, every 7 days with the spraying of 200 times of 1kg microbial bacterias dilution agent once, seedling forms seedling；Hair
A person of good sense is it has surprisingly been found that the control of humidity and room temperature is very important for the effect of the present invention, outside the above range
The technique effect of the present invention will be difficult to by implementing technical scheme under humidity and temperature conditionss.
F, into seedling management：After maca seedling in the step e grows up to seedling, regulation greenhouse humidity is 65%~
70%, regulation and control room temperature on daytime is 20~25 degrees Celsius, and night room temperature is 10~12 degree, and fine day sunshade net opens 50~55cm, more
Cloud or cloudy sunshade net standard-sized sheet, 6~7 hours are opened according to weather side window daily, is highly 20~40cm, 1kg was used every 7 days
The 200 times of sprayings of microbial bacteria dilution agent are once；It is surprisingly found by the inventors that effect of the control of humidity and room temperature for the present invention
It is very important, implementing technical scheme under humidity and temperature conditionss outside the above range will be difficult to
The technique effect of the present invention.
G, hardening：Carry out hardening when leaf grows to 5~6cm, daily 24 hours side window standard-sized sheets, sunshade net standard-sized sheet, every
Every two days with the 200 times of sprayings of 1kg microbial bacterias dilution agent once, the hardening time is 7~15 days, and seedling age is moved after 50~70 days
Plant crop field.
In addition, according to the method for the organic plantation maca of the above embodiment of the present invention, there can also be technology additional as follows
Feature：
The microbial bacterial agent includes bacillus thuringiensis, perlite, coconut palm chaff, mushroom residue, plant ash, soybean cake
(dregs of rice).
According to an embodiment of the invention, raw material of the microbial bacterial agent including following parts by weight is mixed：Su Yun
Golden 1~2 part of bacillus, 10~40 parts of perlite, 10~30 parts of coconut palm chaff, 10~20 parts of mushroom residue, 10~30 parts of plant ash, greatly
20~30 parts of soya-bean cake (dregs of rice).
Further, the proportioning by microbial bacterial agent and nursery soil is to match somebody with somebody 1 cube of nursery per 1kg microbial bacterial agents
Soil.
Further, the seedlings nursing plate be shaped as it is square.
Further, the color of the seedlings nursing plate was black at 2 months to April.
Further, sieved described in the step b, be 60~80 mesh sieves.
Further, watering is using spray atomizing system in the step c, d, e.
Microbial bacterial agent of the present invention, eliminate aphis available for anti-, prevent and treat wheat powdery mildew, prevent and treat eggplant anthracnose,
Healthy and strong, disease-free maca seedling can be cultivated.And shown by the safety evaluatio of microbial bacterial agent, micro- life of the present invention
Thing microbial inoculum is safe and non-toxic.
The present invention is beneficial to be had the technical effect that：There is provided using microbial bacterial agent of the present invention, and using the present invention
Organic plantation maca method, healthy and strong, disease-free maca seedling can be cultivated, be embodied in, seed germination rate is high, survives
Rate is high, and seedling is healthy and strong, and growing way is good after culture transferring, and yield is high, no disease and pests harm.Microbial bacterial agent wherein of the present invention, be in order to
Meet seedling development and particular formulation, its is safe and non-toxic, has stronger retain water and nutrients ability, may be such that plantation comes out
Maca seedling become healthy and strong, and loose ventilation, ensure bed soil and maca seedling no disease and pests harm.
In addition, the method for organic plantation maca provided by the invention compares effect as shown in table 1 below with traditional method：
Table 1
In the second aspect of the present invention, the present invention proposes a kind of microbial bacterial agent, and it includes bacillus thuringiensis, precious
Zhu Yan, coconut palm chaff, mushroom residue, plant ash, soybean cake (dregs of rice).Preferably, the microbial bacterial agent includes the original of following parts by weight
Material is mixed：1~2 part of bacillus thuringiensis, 10~40 parts of perlite, 10~30 parts of coconut palm chaff, 10~20 parts of mushroom residue, grass
10~30 parts of wood ash, 20~30 parts of soybean cake (dregs of rice).Using said preparation, it can effectively implement the above method.Inventor is surprised
Ground finds that the composition of microorganism formulation is very important, microorganism system outside the above range for the effect of the present invention
Technical scheme is implemented in agent will be difficult to the technique effect of the present invention.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
Embodiments of the invention are described below in detail.The embodiments described below is exemplary, is only used for explaining this hair
It is bright, and be not considered as limiting the invention.
Embodiment 1
The invention provides a kind of method of organic plantation maca, comprise the following steps：
A, seedling nutritious soil is prepared：(it is by perlite, turf, decomposed fertilizer, black earth, grass by microbial bacterial agent and nursery soil
Wood ash, calcium superphosphate etc. form) mixed after it is standby；The microbial bacterial agent includes the raw material mixing system of following parts by weight
Into：
The microbial bacterial agent component of table 2 and composition
| Component | Form parts by weight |
| Bacillus thuringiensis | 1 |
| Perlite | 20 |
| Coconut palm chaff | 20 |
| Mushroom residue | 15 |
| Plant ash | 20 |
| Soybean cake (dregs of rice) | 24 |
B, seed treatment：The maca seed that selection is dry, bright, plumpness is good.The removal of impurity is gone in sieving, Celsius with 40
The warm water filtering of degree, washes away the surface of the seed dirt and germ, while further go the removal of impurity.100g microbial bacterial agents are used after filtering
Dilution 200 times of water soaked, seed is pulled out after 20 minutes, wipes the surface of the seed moisture content, be placed on ventilating and cooling place dry it is standby
With.
C, sow：The principal shape of seedlings nursing plate is square, is so easy to moisture content to be more evenly distributed, seedling root system development more fills
Point.The selection of seedlings nursing plate color is also more crucial, black seedlings nursing plate should be selected within 2 months to April, because its light absorptive is good, to seedling root
It is that pastiness is favourable.Emergence is neat, keeps the uniformity of plant seedling growth.Root system is not damaged during transplanting, slow seedling is rapid, survival rate
It is high.First seedlings nursing plate is put neatly, seedling nutritious soil is fitted into seedlings nursing plate, be floating, slight compacting；Watering, at this moment has
Grain, it is necessary to after stirring with being reused after gauze or screen filtration, in order to avoid block shower nozzle；Secondly spray atomizing system is used
Watered, all moistening is defined Nutrition Soil watering time in whole seed plate；Seed is uniformly dialled in disk afterwards, covered
Soil, standard are very thin, uniform one layer；Seed is uniformly dialled in disk afterwards, carries out blinding；Water again, make blinding complete
Full moistening, seed plate is placed in seedling raising greenhouse, into germination period management.
D, period management is planted：Skylight, side window are after planting closed, regulation greenhouse humidity is 65~70%, and regulation and control room temperature on daytime is
26~30 degrees Celsius, night room temperature is 8~10 degrees Celsius, closes sunshade net；Sooner or later ventilation half an hour and watering 15 minutes daily；
Start seedling after 4~5 days；
E, seedling period management：After maca goes out seedling in the step d, regulation greenhouse humidity is 65%~70%, and regulation and control are white
Its room temperature is 26~30 degrees Celsius, and night room temperature is 10~12 degrees Celsius, and sunshade net opens 30~35cm, is easy to adopting for seedling
Light.Ventilation 1~3 hour daily, watering 10~15 minutes sooner or later, every 7 days with 200 times of 1kg microbial bacterias dilution agent spraying one
Secondary, seedling forms seedling；
F, into seedling management：After maca seedling in the step e grows up to seedling, regulation greenhouse humidity is 65%~
70%, regulation and control room temperature on daytime is 20~25 degrees Celsius, and night room temperature is 10~12 degree, and fine day sunshade net opens 50~55cm, more
Cloud or cloudy sunshade net standard-sized sheet, 6~7 hours are opened according to weather side window daily, highly are easy to divulge information for 20~40cm, every
7 days with the spraying of 200 times of 1kg microbial bacterias dilution agent once, other times carry out moisturizing as needed；
G, hardening：Carry out hardening when leaf grows to 5~6cm, daily 24 hours side window standard-sized sheets, sunshade net standard-sized sheet, every
Every two days with the 200 times of sprayings of 1kg microbial bacterias dilution agent once, the hardening time is 7~15 days, and seedling age is moved after 50~70 days
Plant crop field.
The method of microbial bacterial agent and organic plantation maca described in Application Example 1, the emergence number of days of maca seedling is 4
My god, survival rate 100%, gained seedling cotyledon is loose, young stem is thick, well developed root system, determine that root rate is high, the speed of growth is fast, and yield height,
Seedling is stout and strong, and planting survival rates are high, and growth ability is strong, using income approach of the present invention, the organic maca single fruit weight finally obtained
Amount is up to 55g, and average fruit weight is 30-35g.
Embodiment 2
In step a, further the raw material including following weight fraction is made microbial bacterial agent：
The microbial bacterial agent component of table 3 and proportioning
| Component | Match (number) |
| Bacillus thuringiensis | 2 |
| Perlite | 10 |
| Coconut palm chaff | 10 |
| Mushroom residue | 20 |
| Plant ash | 30 |
| Soybean cake (dregs of rice) | 28 |
Other steps are the same as embodiment 1.
The method of microbial bacterial agent and organic plantation maca described in Application Example 2, the emergence number of days of maca seedling is 4
My god, survival rate 100%, gained seedling cotyledon is loose, young stem is thick, well developed root system, determine that root rate is high, the speed of growth is fast, and yield height,
Seedling is stout and strong, and planting survival rates are high, and growth ability is strong, using income approach of the present invention, the organic maca single fruit weight finally obtained
Amount is up to 55g, and average fruit weight is 30-35g.
Embodiment 3
In step a, further the raw material including following weight fraction is made microbial bacterial agent：
The microbial bacterial agent component of table 4 and proportioning
| Component | Match (number) |
| Bacillus thuringiensis | 2 |
| Perlite | 40 |
| Coconut palm chaff | 10 |
| Mushroom residue | 10 |
| Plant ash | 10 |
| Soybean cake (dregs of rice) | 28 |
Other steps are the same as embodiment 1.
The method of microbial bacterial agent and organic plantation maca described in Application Example 3, the emergence number of days of maca seedling is 4
My god, survival rate 100%, gained seedling cotyledon is loose, young stem is thick, well developed root system, determine that root rate is high, the speed of growth is fast, and yield height,
Seedling is stout and strong, and planting survival rates are high, and growth ability is strong, using income approach of the present invention, the organic maca single fruit weight finally obtained
Amount is up to 55g, and average fruit weight is 30-35g.
Embodiment 4
In step a, further the raw material including following weight fraction is made microbial bacterial agent：
The microbial bacterial agent component of table 5 and proportioning
| Component | Match (number) |
| Bacillus thuringiensis | 1.5 |
| Perlite | 13.5 |
| Coconut palm chaff | 30 |
| Mushroom residue | 15 |
| Plant ash | 20 |
| Soybean cake (dregs of rice) | 20 |
Other steps are the same as embodiment 1.
The method of microbial bacterial agent and organic plantation maca described in Application Example 4, the emergence number of days of maca seedling is 4
My god, survival rate 100%, gained seedling cotyledon is loose, young stem is thick, well developed root system, determine root rate height, the speed of growth fast, and yield
Height, seedling is stout and strong, and planting survival rates are high, and growth ability is strong, using income approach of the present invention, the organic maca single fruit finally obtained
Weight is up to 55g, and average fruit weight is 30-35g.
Embodiment 5
In step a, further the raw material including following weight fraction is made microbial bacterial agent：
The microbial bacterial agent component of table 6 and proportioning
| Component | Match (number) |
| Bacillus thuringiensis | 1 |
| Perlite | 14 |
| Coconut palm chaff | 30 |
| Mushroom residue | 15 |
| Plant ash | 10 |
| Soybean cake (dregs of rice) | 30 |
Other steps are the same as embodiment 1.
The method of microbial bacterial agent and organic plantation maca described in Application Example 5, the emergence number of days of maca seedling is 4
My god, survival rate 100%, gained seedling cotyledon is loose, young stem is thick, well developed root system, determine that root rate is high, the speed of growth is fast, and yield height,
Seedling is stout and strong, and planting survival rates are high, and growth ability is strong, using income approach of the present invention, the organic maca single fruit weight finally obtained
Amount is up to 55g, and average fruit weight is 30-35g.
Microbial bacterial agent functional experiment is tested：
Embodiment 6：It is anti-to eliminate aphis
Aphid, also known as aphid, aphid, are a kind of plant-feed insects, including Aphidoidea (also known as aphid Superfamily, scientific name：
Aphidoidea all members under).About 4400 kinds of a total of 10 sections of aphid having now been found that, wherein majority belongs to aphid
Section.Aphid is also one of most destructive insect on the earth.Wherein about 250 kinds are for agriculture and forestry and horticulture harm
Serious insect.
The microbial bacterial agent that embodiment 1 provides, is diluted with water to 10 times of volume multiple, the sprinkling of the fine day morning, and next day is multiple
Look into discovery, dead larvae, by taking 100 plant with aphid as an example, after sprinkling, effective percentage 97%.
Embodiment 7：Prevent and treat wheat powdery mildew
Powdery mildew, it can be fallen ill from seedling to heading.Main wheat leaf blade of causing harm, also cause harm stem and tassel.On blade
Start to produce yellow dot, then expand and develop into circular or oval scab, there is the mould layer of white powder on surface.Ordinary circumstance
Lower blade is more than upper blade, and vacuum side of blade is more than front.Mildew early stage is individually scattered, after be unified into a big mildew, very
Full leaf can be extremely covered, has a strong impact on photosynthesis, eubolism is interfered, causes early ageing, yield incurs loss.
The microbial bacterial agent of the offer of the embodiment of the present invention 2 is provided, is diluted with water to 18 times of volume multiple, the spray of the fine day morning
Spill, checked after three days, scab reduces.After seven days, plant recovers normal.After 100 wheat powdery mildew plant sprinklings, find effective
Rate 95%.Culture medium microscopy, it is found that the present invention has obvious inhibitory action to powdery mildew.
Embodiment 8:The preventing and treating of eggplant anthracnose
Eggplant anthracnose harm disease fruit eggplant anthracnose main harm fruit, at the beginning fruit surface produce subcircular,
Ellipse or irregular shape, dark brown, slightly concave sunken scab.Scab constantly expands, or scab converges can form large-scale scab, has
When extend to half of fruit.The dense black dot in later stage disease portion surface, germanium red mucilage is overflowed when moist.The sick subcutaneous pulp in portion
It is micro- brown, dry rot shape, whole fruit rot can be caused when serious.Its pathogenic germ is with mycelium and aceravlus with invalid
Body is survived the winter in soil, can also conidium be attached to the surface of the seed and survive the winter.Next year is produced mitogenetic by aceravlus of surviving the winter
Spore, propagated to by means of rainwater sputtering on the fruit of plant bottom and cause morbidity, the infected seed of sowing can infect seedling when sprouting
It is allowed to fall ill.
The plot that the present invention uses, eggplant anthracnose is broken out on a large scale before this, when this year plants eggplant again：Use step
Prevention and control of plant diseases, pest control medicine obtained by C, 5 times of volume multiple is diluted with water to, soaked seed 1-2 hours, sowing.
After germination, using the gained microbial bacterial agent of embodiment 3,15 times of volume multiple are diluted with water to, are filled
Root operates.After eggplant fallen flowers result, using the gained microbial bacterial agent of embodiment 3,25 times of volume multiple are diluted with water to, are carried out
Spray eggplant fruit operation.
Harvested to eggplant, find 500 eggplant plant, it is ill less than 1%.It can substantially prove the present invention to eggplant charcoal
The pathogenic germ of subcutaneous ulcer disease has obvious killing action with mycelium and aceravlus.Its therapeutic effect, as shown in table 7：
Preventing and treating experimental data of the microbial bacterial agent of table 7 to eggplant anthracnose
| Name of disease | Germ | Recur disease hole count | Recurrence rate (%) | Prevention effect (%) |
| Bacterial septicaemia syndrome | Drupe Xanthomonas campestris | 2 | 10 | 90 |
| Mildew shothole disease | Thermophilic fruit knife spore is mould | 8 | 27 | 73 |
| Foxiness shothole disease | Drupe vacation tail spore | 7 | 20 | 80 |
Safety assessment is tested
Present invention also offers microbial bacterial agent safety evaluatio of the present invention experiment, tried by Mouse Acute Toxicity
Test, PCEMNR number micronucleus test, mouse inbred strain is to a kind of microbial bacteria provided by the invention
The security performance of agent is evaluated.
Embodiment 9：
Acute toxicity test in mice
Test method：
To the SPF level Kunming mouse acute oral toxicity tests of two kinds of sexes, method reference《Health food is examined with commenting
Valency technical specification》The toxicology method of inspection of 2003 editions, gavage twice is carried out to 15 female mouse and 15 male mouse respectively,
Each dosage is 20g/kg BW (equivalent to 667 times of crowd's recommended intake 0.03g/kgBW), by the observation period of 14 days,
Result of the test is shown in Table 8.
8 microbial bacterial agent of the present invention of table is to acute toxicity test in mice result
Experimental data shows that two groups of animals do not observe Novel presentation, and animal poisoning symptom and dead hair are had no in two weeks
It is raw.Evaluated by acute toxicity grading criteria, the given the test agent belongs to nontoxic level.
Embodiment 10：
PCEMNR number micronucleus test
To the SPF level Kunming mouse Polychromatic erythrocyte number micronucleus tests of two kinds of sexes, method is pressed《Health food
Examine and assessment technique specification》The toxicology method of inspection of 2003 editions, the results are shown in Table 9.
9 microbial bacterial agent of the present invention of table is to PCEMNR number micronucleus test result
Note：The * P ＜ 0.01 compared with negative control group
Experimental data shows, compared with negative control group, positive controls male and female micronuclei in mice rate there were significant differences property (P
＜ 0.01), and each dosage group male and female micronuclei in mice rate of given the test agent is compared with negative control group, difference that there are no significant (P ＞
0.05), and in normal range (NR), show the given the test agent bone marrow micronucleus test result for feminine gender.
Embodiment 11：
Mouse inbred strain
To SPF level Kunming mouse sperm malformation tests, method is pressed《Health food is examined and assessment technique specification》2003
The toxicology method of inspection of version, the results are shown in Table 10.
10 microbial bacterial agent of the present invention of table is to mouse inbred strain result
Note：The * P ＜ 0.01 compared with negative control group
Experimental data shows, compared with negative control group A, positive controls mouse sperm deformity rate has significant difference (P
＜ 0.01), and each dosage group mouse sperm deformity rate of given the test agent is compared with negative control group, difference that there are no significant (P ＞
0.05), show the given the test agent sperm malformation test result for feminine gender.
Each experimental data, inventor are drawn to draw a conclusion with reference to more than, and a kind of microbial bacterial agent provided by the invention is peace
Atoxic.
Embodiment 12：
It is as follows that a kind of method of organic plantation maca provided by the invention is compareed effect by the present inventor with traditional method
Shown in table 11：
Table 11
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Necessarily refer to identical embodiment or example.Moreover, specific features, structure, material or the feature of description can be any
One or more embodiments or example in combine in an appropriate manner.
Although an embodiment of the present invention has been shown and described, it will be understood by those skilled in the art that：Not
In the case of departing from the principle and objective of the present invention a variety of change, modification, replacement and modification can be carried out to these embodiments, this
The scope of invention is limited by claim and its equivalent.
Claims (7)
- A kind of 1. method of organic plantation maca, it is characterised in that comprise the following steps：A, seedling nutritious soil is prepared：It is standby after microbial bacterial agent and nursery soil are mixed, the nursery soil be by perlite, Turf, decomposed fertilizer, black earth, plant ash, calcium superphosphate composition；B, seed treatment：The maca seed that selection is dry, bright, plumpness is good, sieving, with 40 degrees Celsius of warm water mistake Filter, is soaked with 200 times of water of 100g microbial bacterias dilution agent after filtering, pulls seed out after 20 minutes, wipe the surface of the seed Moisture content, be placed on ventilating and cooling place dry it is standby；C, sow：Seedling nutritious soil is fitted into seedlings nursing plate, be floating, slight compacting；Watering, makes Nutrition Soil in whole seedlings nursing plate complete Portion moistens；Seed is uniformly dialled in disk afterwards, carries out blinding；Water again, blinding is moistened completely, seedlings nursing plate is placed on In seedling raising greenhouse, into germination period；D, period management is planted：Skylight, side window are after planting closed, regulation greenhouse humidity is 65~70%, regulation and control room temperature on daytime is 26~ 30 degrees Celsius, night room temperature is 8~10 degrees Celsius, closes sunshade net；Sooner or later ventilation half an hour and watering 15 minutes daily；4~5 Start seedling after it；E, seedling period management：After maca goes out seedling in the step d, regulation greenhouse humidity is 65%~70%, regulation and control room on daytime Temperature is 26~30 degrees Celsius, and night room temperature is 10~12 degrees Celsius, and sunshade net opens 30~35cm, is taken a breath 1~3 hour daily, Sooner or later watering 10~15 minutes, every 7 days with the spraying of 200 times of 1kg microbial bacterias dilution agent once, seedling forms seedling；F, into seedling management：After maca seedling in the step e grows up to seedling, regulation greenhouse humidity is 65%~70%, is adjusted It is 20~25 degrees Celsius to control room temperature on daytime, and night room temperature is 10~12 degree, and fine day sunshade net opens 50~55cm, cloudy or cloudy Sunshade net standard-sized sheet, 6~7 hours are opened according to weather side window daily, are highly 20~40cm, every 7 days with 1kg microbial bacterias 200 times of sprayings of dilution agent are once；G, hardening：Hardening, daily 24 hours side window standard-sized sheets, sunshade net standard-sized sheet, at interval of one are carried out when leaf grows to 5~6cm It is sprayed once for 200 times with 1kg microbial bacterias dilution agent, and the hardening time is 7~15 days, and seedling age carries out transplanting greatly after 50~70 days Field,Wherein, raw material of the microbial bacterial agent including following parts by weight is mixed：1~2 part of bacillus thuringiensis, it is precious 10~40 parts of pearl rock, 10~30 parts of coconut palm chaff, 10~20 parts of mushroom residue, 10~30 parts of plant ash, 20~30 parts of soybean cake.
- 2. the method for organic plantation maca according to claim 1, it is characterised in that described by microbial bacterial agent and nursery The proportioning of soil is to match somebody with somebody 1 cube of nursery soil per 1kg microbial bacterial agents.
- 3. the method for organic plantation maca according to claim 1, it is characterised in that the side of being shaped as of the seedlings nursing plate Shape.
- 4. the method for organic plantation maca according to claim 1, it is characterised in that the color of the seedlings nursing plate was at 2 months It is black to April.
- 5. the method for organic plantation maca according to claim 1, it is characterised in that：Sieved described in the step b, be 60~80 mesh sieves.
- 6. the method for organic plantation maca according to claim 1, it is characterised in that pour hydromining in the step c, d, e It is spray atomizing system.
- 7. a kind of microbial bacterial agent, it is characterised in that the raw material that the microbial bacterial agent includes following parts by weight is mixed： 1~2 part of bacillus thuringiensis, 10~40 parts of perlite, 10~30 parts of coconut palm chaff, 10~20 parts of mushroom residue, plant ash 10~30 Part, 20~30 parts of soybean cake.
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| CN105453883B (en) * | 2016-01-12 | 2019-03-01 | 武汉华士特工业生物技术开发有限公司 | A kind of breeding method of maca |
| CN105940896B (en) * | 2016-04-27 | 2019-06-14 | 小金县鑫鸿玛卡生物科技有限公司 | The method that Maca seedling is efficiently bred |
| CN106723021A (en) * | 2016-11-28 | 2017-05-31 | 武汉哈福科技有限公司 | A kind of functional food for improving the health care of sleep |
| CN106888620A (en) * | 2017-03-02 | 2017-06-27 | 张瑞明 | A kind of raising old kind of method of survival rate of seedling of agate card |
| CN109997650B (en) * | 2019-05-14 | 2021-06-29 | 南京林业大学 | Culture medium for ryegrass and preparation method thereof |
| CN110622757A (en) * | 2019-09-12 | 2019-12-31 | 邳州市苏鲁粮种繁育专业合作社 | Direct maca sowing and cultivating technology |
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