CN108739675A - A kind of method of double spot grace aphid chalcid fly biological controls - Google Patents

A kind of method of double spot grace aphid chalcid fly biological controls Download PDF

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Publication number
CN108739675A
CN108739675A CN201810411727.7A CN201810411727A CN108739675A CN 108739675 A CN108739675 A CN 108739675A CN 201810411727 A CN201810411727 A CN 201810411727A CN 108739675 A CN108739675 A CN 108739675A
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aleyrodid
aphid chalcid
double spot
grace
host plant
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CN108739675B (en
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邱宝利
凡泽云
何瞻
陈晓胜
王兴民
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South China Agricultural University
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South China Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
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Abstract

The invention discloses a kind of biological control methods of aleyrodid pest obligatory parasitism bee-bis- spots grace aphid chalcid fly, by being completed in independent greenhouse in cleaning planting room, aleyrodid transfer room, aleyrodid breeding room, double spot grace aphid chalcid fly transfer rooms and five, separation chamber of aleyrodid-Shuan Banenya chalcid flies.The method is followed successively by:The plantation of host plant tobacco, cotton, tomato, cabbage mustard and poinsettia is cultivated;Different host plant aleyrodid inoculations;Aleyrodid develops on different host plants, the inoculation of double spot grace aphid chalcid flies, the bis- spot grace aphid chalcid flies separation of aleyrodid-, bee pupa is collected, pupa box makes preservation etc..It may be implemented the biological control of double spot grace aphid chalcid flies by above technical scheme, more host plants cultivate the adaptability to host when can increase the practical prevention aleyrodid of double spot grace aphid chalcid flies, to improve its parasitism efficiency.

Description

A kind of method of double spot grace aphid chalcid fly biological controls
Technical field
The present invention relates to agriculture and forestry biological control of insect pests fields, more particularly, to a kind of scale of double spot grace aphid chalcid flies Change propagation method.
Background technology
In recent years, with global warming, the generation and harm of the aleyrodids pest such as Bemisia tabaci and Trialeurodes vaporariorum Westwood are year by year It aggravates, since it is extensive with host range, the features such as development of drug resistance is rapid, serious commercial is caused to crops and flowers Loss.Currently, pesticide control is still to control the main means of aleyrodid class pest, but the use of pesticide not only has residual, dirt The problems such as dye environment, pest resistance to insecticide increase can also kill natural enemy insect.Therefore, the biological preventing control method of aleyrodid becomes and currently grinds Study carefully the hot spot with application.
Double spot grace Aphelinus Hymenoptera aphid Chalcididaes are one of the advantage parasitic wasps of South China's aleyrodid class pest, It plays an important role, has a good application prospect to the prevention and control of aleyrodid class pest in nature.It is small with double spot grace aphids Bee prevents the green bio Prevention Technique of aleyrodid class pest, is gradually concerned by people.But up to the present, not yet There is the research of the large-scale production of double spot grace aphid chalcid flies so that double spot grace aphid chalcid flies receive the control action of aleyrodid class pest Greatly limit.Therefore, double spot grace aphid chalcid fly large-scale production technologies are studied, the biological control application of aleyrodid class pest is carried out, With good economic, ecology and social effect.
Invention content
The technical problem to be solved by the present invention is to supplement the blank of double spot grace aphid chalcid fly reproduction techniques, a kind of double spots are provided The method of grace aphid chalcid fly biological control.
The above-mentioned purpose of the present invention is to give realization by the following technical programs:
A kind of biological control method of double spot grace aphid chalcid flies, includes the following steps:
S1. host plant is planted:Plantation is for different types of host plant needed for aleyrodid growth reproduction;
S2. aleyrodid is inoculated with:Different types of host plant that S1 grows fine is respectively put into the insect cage of aleyrodid transfer room, The aleyrodid of spawning period is inoculated on host plant by blade cage again, controls every host plant blades adult whitefly quantity It it is 80~100, after allowing its breeding to lay eggs 24 hours, with 30~40 ovum/cm2It is advisable, then catches up with all adult whiteflies It walks, 22~28 DEG C of room temperature of control inoculation, humidity 60%~80%;
S3. aleyrodid breeds:The host plant that S2 has been inoculated with to aleyrodid ovum moves in aleyrodid breeding room and uniformly places, and allows aleyrodid ovum Hatching development;If there is residual adult whitefly on host plant, DDVP paper slip can be used 4 hours stifling, kill the aleyrodid left Adult;22~28 DEG C of room temperature of control breeding, humidity 60%~80%, wait for that vacuum side of blade aleyrodid nymph quantity was developed to for 2~3 ages When, it can be used for the inoculation of aphid chalcid fly;
S4. double spot grace aphid chalcid fly queen bee breedings:The host plant of 2~3 age aleyrodid nymph of band in S3 is moved into double spot grace aphid chalcid flies Transfer room, by bee:Aleyrodid 1:The 20 double spot grace aphid chalcid fly queen bees of access post-coitum, remove bee after 2~3 days, connect bee indoor temperature and exist 22~28 DEG C, humidity 60%~80% can be obtained the female pupa of double spot aphid chalcid flies after 10~11 days;
S5. double spot grace aphid chalcid fly drone breedings:It will be carried by the host plant of 3 age aleyrodid nymphs of double spot aphid chalcid flies parasitism in S4 Double spot grace aphid chalcid fly drone breeding rooms are moved to, statistics is by the quantity of parasitic aleyrodid nymph, according to by parasitic aleyrodid nymph:Double spots Grace aphid chalcid fly=5:1 ratio accesses the latter, allows its hyperparasite previously by parasitic aleyrodid nymph, obtains in this manner double Spot grace aphid chalcid fly drone;Control connects 22~28 DEG C of bee indoor temperature, humidity 60%~80%, and double spot grace are can be obtained after 10~11 days Aphid chalcid fly hero pupa;
S6. double spot grace aphid chalcid fly inoculation breedings:The host plant that 2~3 age aleyrodid nymphs are carried in S3 is uniformly placed on parasitism In bee transfer room, by parasitic wasp of the emergence time in S4, S5 within 24 hours by female:Male=2:1 carries out population in advance mixes It closes, allows double spot grace aphid chalcid fly pangamies 24~48 hours;Then according to aleyrodid:Parasitic wasp=20:Aphid chalcid fly is accessed blade by 1 Cage, allows double spot grace aphid chalcid fly parasitism aleyrodid nymphs, and parasitic wasp is removed after 4~5 days;Control connects bee breeding indoor temperature 22~28 DEG C, humidity 60%~80%;
S7. aleyrodid-aphid chalcid fly separation chamber:It is even at this time to have not by the aleyrodid of double spot grace aphid chalcid fly parasitisms plumage after 8~12 days Adult is turned to, is left puppet puparium, plant can be moved to aleyrodid-aphid chalcid fly separation chamber at this time, host plant blades can be picked, dried in the shade Bee box is made afterwards, or the small bee pupa card of double spot grace aphids is made after washing away with water, is placed in 8~10 DEG C of refrigerator storages.
The host plant that the propagation method of the present invention uses it is numerous in variety, mixing raising aleyrodid then raise pair spot grace aphids Chalcid fly can improve host's adaptability of parasitic wasp, to improve the parasitic rate in its application process.In addition, host plant leaf Piece is more, big, blade face is smooth, is conducive to aleyrodid and expands numerous and bee box to make preferably, host plant described in S1 be tobacco, cotton, At least two in cabbage mustard and poinsettia.
Preferably, described tobacco 8 or more true leaf, wait for that 6, cotton or more true leaf, wild cabbage 6 or more true leaf, poinsettia are cut It is spare after spraying DDVP desinsection one month except old leaf.
Preferably, host plant described in S1 is all made of potting mode and plants, convenient for management.
Preferably, in host plant plantation room, aleyrodid transfer room, aleyrodid breeding room, double spot grace aphid chalcid flies inoculation breeding room The distance between every potted plant interval is 20~30 centimetres.
Preferably, host plant plantation room, aleyrodid transfer room, aleyrodid breeding room, the inoculation breeding of double spot grace aphid chalcid flies Room, aleyrodid-five, aphid chalcid fly separation chamber greenhouse are not interfere with each other separately independently, and indoor conditions is controlled at 22~28 DEG C(It is preferred that 26℃), RH60%~80%(It is preferred that 65%), illumination shading value L:D=14:10.
Compared with existing other aphid chalcid fly reproduction techniques, the invention has the advantages that:
(1)Double spot grace aphid chalcid flies are the advantage parasitic wasps of South China aleyrodid pest, to powder in protecting field or industrialized agriculture The biological control significant effect of lice pest.
(2)The large-scale breeding method of double spot grace aphid chalcid flies of the present invention, it is various using host plant, resist other insects And the ability of disease infestation is stronger than single host plant, meanwhile, a variety of host plant raising aleyrodids then raise parasitic wasp, can To improve host's adaptability of parasitic wasp, the parasitic rate in its actual application is improved.
(3)The large-scale breeding method of double spot grace aphid chalcid flies of the present invention, method is simple, easily grasps, it can be achieved that double spot grace The whole year uninterrupted breeding of aphid chalcid fly, though not the keeper of professional knowledge if can learn in a short time;Feeding cost It is cheap, after large-scale production obtains putting bee box or putting bee card for field prevention and control aleyrodid, huge society, ecology can be brought And economic benefit.
Specific implementation mode
Further illustrated the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagent, methods and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are purchased in market.
Embodiment 1
(1)Facility prepares
Build host plant plantation room, numerous room is expanded in aleyrodid transfer room, aleyrodid breeding room, the inoculation of double spot grace aphid chalcid flies, aleyrodid-aphid is small Bee separation chamber:Area depends on the needs, general 30~50m2, exhaust fan and 1.5 air conditioner control temperature are respectively mounted, and The foster worm frame of different number is put according to size, and time switch control is installed and supports worm bracket lamp pipe switch time.
Support worm frame:The foster worm frame of 165cm*130cm*75cm several, each shelf point two layers, every layer of top installation 6 The fluorescent lamp of 28w each supports every layer of worm frame and installs a manual switch respectively.
(2)Rearing conditions
Temperature, humidity and illumination condition:Host plant culturing room, aleyrodid transfer room, aleyrodid expand numerous room, parasitic wasp transfer room and The indoor conditions for expanding numerous room is controlled at 22~28 DEG C(26 DEG C best), RH60%~80%(65% is best), illumination shading value L: D=14:10。
(3)Worm sources prepare
Bemisia tabaci:Worm sources come from engineering center of the biological control Ministry of Education(Agricultural University Of South China)
Double spot grace aphid chalcid flies:Worm sources come from engineering center of the biological control Ministry of Education(Agricultural University Of South China)
(4)Host plant is cultivated
Tobacco coating seed(PVH19)It is purchased from Weifang City of Shandong Province kind for the production of Yun Xizhongyan seeds Co., Ltd, cotton Sub- management station, wild cabbage are purchased from seed control station of Weifang City of Shandong Province, poinsettia potting is purchased from the villages Fo Shanchen flowers market, by cotton Flower, tobacco and wild cabbage carry out nursery, and the seedling brought out is transplanted to by batch in plant culturing room and carries out potting, by poinsettia into Row, which wipes out old leaf and sprays DDVP, carries out desinsection processing, waits for tobacco 8 or more true leaf, waits for 6, cotton or more true leaf, wild cabbage 6 The above true leaf, poinsettia spray are spare when placing one month or more.Tobacco, cotton and cabbage mustard were transplanted in transplanting every 20 days Once, poinsettia is periodically bought, it is ensured that to have host plant spare throughout the year.
(5)Bemisia tabaci is inoculated with
It is respectively put into step in the insect cage of Bemisia tabaci transfer room(4)The different types of host plant to grow fine, then lead to It crosses blade cage the Bemisia tabaci of spawning period is inoculated on host plant, every host plant blades adult whitefly quantity of control is After 80~100 allow its breeding to lay eggs 24 hours, with 30-40 ovum/cm2It is advisable, then catches up with all Bemisia tabaci adults It walks., inoculation room temperature control is in 22~28 DEG C, humidity 60%~80%.
(6)Bemisia tabaci is bred
By step(5)The host plant for being inoculated with tobacco powder nit moves in Bemisia tabaci breeding room and uniformly places, and allows tobacco powder nit Hatching development.If checking on host plant there are residual Bemisia tabaci adults, DDVP paper slip can be used 4 hours stifling, kill is left Bemisia tabaci adults.Room temperature control is bred in 22~28 DEG C, humidity 60%~80%.Vacuum side of blade Bemisia tabaci Nymph quantity to be made When being developed to for 2~3 age, it can be used for double spot grace aphid chalcid fly inoculations.
(7)Double spot grace aphid chalcid fly queen bee breedings
By step(6)The host plant of middle band 2~3 age aleyrodid nymph moves to double spot grace aphid chalcid fly transfer rooms, by bee:Aleyrodid 1:20 Access the double spot grace aphid chalcid fly queen bees of post-coitum, bee removed after 2~3 days, connect bee indoor temperature 22~28 DEG C, humidity 60%~ It can be obtained the female pupa of double spot aphid chalcid flies after 80%, 10~11 days.
(8)Double spot grace aphid chalcid fly drone breedings
By step(7)In with moving to pair spot grace aphids by the host plant of the 2-3 age Bemisia tabaci Nymphs of double spot grace aphid chalcid fly parasitism Chalcid fly drone breeds room, and statistics is by the quantity of parasitic Bemisia tabaci Nymph, according to by parasitic Bemisia tabaci Nymph:Double spot grace aphid chalcid flies =5:1 ratio accesses the latter, allows its hyperparasite previously by parasitic Bemisia tabaci Nymph, obtains double spot grace aphids in this manner Chalcid fly drone.22~28 DEG C of bee indoor temperature, humidity 60%~80% are connect, it is male that double spot grace aphid chalcid flies are can be obtained after 10~11 days Pupa.
(9)Double spot grace aphid chalcid fly inoculation breedings:By step(6)In uniformly put with the host plants of 2-3 age aleyrodid nymphs It sets in parasitic wasp transfer room, by step(7),(8)In parasitic wasp of the emergence time within 24 hours by female:Male=2:1 is pre- The mixing of first Advanced group species, allows double spot grace aphid chalcid fly pangamies 24~48 hours.Then according to Bemisia tabaci:Parasitic wasp=20:1 will Aphid chalcid fly accesses blade cage, allows double spot grace aphid chalcid fly parasitism Bemisia tabaci Nymphs, and parasitic wasp is removed after 4~5 days.It is indoor to connect bee breeding 22~28 DEG C of temperature, humidity 60%~80%.
(10)Bemisia tabaci-Shuan Banenya chalcid flies separation chamber
After about 10 days, occasionally have adult has not been turned by the Bemisia tabaci of double spot grace aphid chalcid fly parasitisms at this time, is left puppet puparium.This When plant can be moved to Bemisia tabaci-Shuan Banenya chalcid flies separation chamber, host plant blades can be picked, after drying in the shade make bee box, or The small bee pupa card of double spot grace aphids is made after washing away with water, is placed in 8~10 DEG C of refrigerator storages.

Claims (5)

1. a kind of biological control method of double spot grace aphid chalcid flies, which is characterized in that include the following steps:
S1. host plant is planted:Plantation is for different types of host plant needed for aleyrodid growth reproduction;
S2. aleyrodid is inoculated with:Different types of host plant that S1 grows fine is respectively put into the insect cage of aleyrodid transfer room, The aleyrodid of spawning period is inoculated on host plant by blade cage again, controls every host plant blades adult whitefly quantity It it is 80~100, after allowing its breeding to lay eggs 24 hours, with 30~40 ovum/cm2It is advisable, then catches up with all adult whiteflies It walks, 22~28 DEG C of room temperature of control inoculation, humidity 60%~80%;
S3. aleyrodid breeds:The host plant that S2 has been inoculated with to aleyrodid ovum moves in aleyrodid breeding room and uniformly places, and allows aleyrodid ovum Hatching development;If there is residual adult whitefly on host plant, DDVP paper slip can be used 4 hours stifling, kill the aleyrodid left Adult;22~28 DEG C of room temperature of control breeding, humidity 60%~80%, wait for that vacuum side of blade aleyrodid nymph quantity was developed to for 2~3 ages When, it can be used for the inoculation of aphid chalcid fly;
S4. double spot grace aphid chalcid fly queen bee breedings:The host plant of 2~3 age aleyrodid nymph of band in S3 is moved into double spot grace aphid chalcid flies Transfer room, by bee:Aleyrodid 1:The 20 double spot grace aphid chalcid fly queen bees of access post-coitum, remove bee after 2~3 days, connect bee indoor temperature and exist 22~28 DEG C, humidity 60%~80% can be obtained the female pupa of double spot aphid chalcid flies after 10~11 days;
S5. double spot grace aphid chalcid fly drone breedings:It will be carried by the host plant of 3 age aleyrodid nymphs of double spot aphid chalcid flies parasitism in S4 Double spot grace aphid chalcid fly drone breeding rooms are moved to, statistics is by the quantity of parasitic aleyrodid nymph, according to by parasitic aleyrodid nymph:Double spots Grace aphid chalcid fly=5:1 ratio accesses the latter, allows its hyperparasite previously by parasitic aleyrodid nymph, obtains in this manner double Spot grace aphid chalcid fly drone;Control connects 22~28 DEG C of bee indoor temperature, humidity 60%~80%, and double spot grace are can be obtained after 10~11 days Aphid chalcid fly hero pupa;
S6. double spot grace aphid chalcid fly inoculation breedings:The host plant that 2~3 age aleyrodid nymphs are carried in S3 is uniformly placed on parasitism In bee transfer room, by parasitic wasp of the emergence time in S4, S5 within 24 hours by female:Male=2:1 carries out population in advance mixes It closes, allows double spot grace aphid chalcid fly pangamies 24~48 hours;Then according to aleyrodid:Parasitic wasp=20:Aphid chalcid fly is accessed blade by 1 Cage, allows double spot grace aphid chalcid fly parasitism aleyrodid nymphs, and parasitic wasp is removed after 4~5 days;Control connects bee breeding indoor temperature 22~28 DEG C, humidity 60%~80%;
S7. aleyrodid-aphid chalcid fly separation chamber:After 8~12 days, occasionally has do not sprouted wings by the aleyrodid of double spot grace aphid chalcid fly parasitisms at this time For adult, it is left puppet puparium, plant can be moved to aleyrodid-aphid chalcid fly separation chamber at this time, host plant blades can be picked, after drying in the shade Bee box is made, or the small bee pupa card of double spot grace aphids is made after washing away with water, is placed in 8~10 DEG C of refrigerator storages.
2. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that host plant described in S1 is cigarette At least two in grass, cotton, cabbage mustard and poinsettia.
3. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that host plant described in S1 is adopted It is planted with potting mode.
4. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that described tobacco 8 or more is true It is leaf, spare after 6, cotton or more true leaf, wild cabbage 6 or more true leaf, poinsettia wipe out old leaf spray DDVP desinsection one month.
5. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that double spot grace aphid chalcid flies are Lonely female infertility, both sexes produce female mode of reproduction, and drone need to be of the same race through hyperparasite or belongs to the acquisition of sibling species aphid chalcid fly.
CN201810411727.7A 2018-05-02 2018-05-02 Large-scale breeding method for Encarsia bimaculata Active CN108739675B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110338154A (en) * 2019-08-09 2019-10-18 河南科技大学 For breeding vector plant system and construction method, the propagation method and the method for preventing and treating aleyrodid class pest of pale yellow grace aphid chalcid fly

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101390505A (en) * 2008-10-28 2009-03-25 北京市农林科学院 Encarsia Sophia artificial propagation production method
KR20120071946A (en) * 2010-12-23 2012-07-03 대한민국(농촌진흥청장) Mass rearing of geocoris pallidipennis
US20150030689A1 (en) * 2011-09-20 2015-01-29 Koppert B.V. Phytoseiid predatory mite releasing system and method for production
CN107396891A (en) * 2017-08-22 2017-11-28 中国农业科学院植物保护研究所 A kind of method that constant low temperature stores the pale yellow female pupa of grace aphid chalcid fly

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101390505A (en) * 2008-10-28 2009-03-25 北京市农林科学院 Encarsia Sophia artificial propagation production method
KR20120071946A (en) * 2010-12-23 2012-07-03 대한민국(농촌진흥청장) Mass rearing of geocoris pallidipennis
US20150030689A1 (en) * 2011-09-20 2015-01-29 Koppert B.V. Phytoseiid predatory mite releasing system and method for production
CN107396891A (en) * 2017-08-22 2017-11-28 中国农业科学院植物保护研究所 A kind of method that constant low temperature stores the pale yellow female pupa of grace aphid chalcid fly

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
钱明惠等: "双斑恩蚜小蜂的生殖方式及其在烟粉虱体内的发育", 《应用昆虫学报》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110338154A (en) * 2019-08-09 2019-10-18 河南科技大学 For breeding vector plant system and construction method, the propagation method and the method for preventing and treating aleyrodid class pest of pale yellow grace aphid chalcid fly

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