CN108739675A - A kind of method of double spot grace aphid chalcid fly biological controls - Google Patents
A kind of method of double spot grace aphid chalcid fly biological controls Download PDFInfo
- Publication number
- CN108739675A CN108739675A CN201810411727.7A CN201810411727A CN108739675A CN 108739675 A CN108739675 A CN 108739675A CN 201810411727 A CN201810411727 A CN 201810411727A CN 108739675 A CN108739675 A CN 108739675A
- Authority
- CN
- China
- Prior art keywords
- aleyrodid
- aphid chalcid
- double spot
- grace
- host plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000317929 Chalcididae Species 0.000 title claims abstract description 89
- 241001124076 Aphididae Species 0.000 title claims abstract description 85
- 238000000034 method Methods 0.000 title claims abstract description 17
- 241000196324 Embryophyta Species 0.000 claims abstract description 55
- 238000009395 breeding Methods 0.000 claims abstract description 31
- 230000001488 breeding effect Effects 0.000 claims abstract description 29
- 238000012546 transfer Methods 0.000 claims abstract description 16
- 238000011081 inoculation Methods 0.000 claims abstract description 14
- 230000024241 parasitism Effects 0.000 claims abstract description 13
- 241000382353 Pupa Species 0.000 claims abstract description 11
- 238000000926 separation method Methods 0.000 claims abstract description 10
- 229920000742 Cotton Polymers 0.000 claims abstract description 8
- 240000007124 Brassica oleracea Species 0.000 claims abstract description 4
- 244000299507 Gossypium hirsutum Species 0.000 claims abstract 3
- 244000061176 Nicotiana tabacum Species 0.000 claims abstract 2
- 230000003071 parasitic effect Effects 0.000 claims description 25
- 241000819999 Nymphes Species 0.000 claims description 15
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 9
- 241000221079 Euphorbia <genus> Species 0.000 claims description 8
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 241000258937 Hemiptera Species 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- 241000238631 Hexapoda Species 0.000 claims description 6
- OEBRKCOSUFCWJD-UHFFFAOYSA-N dichlorvos Chemical compound COP(=O)(OC)OC=C(Cl)Cl OEBRKCOSUFCWJD-UHFFFAOYSA-N 0.000 claims description 6
- 235000006008 Brassica napus var napus Nutrition 0.000 claims description 5
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 claims description 5
- 241001674939 Caulanthus Species 0.000 claims description 5
- 241000596168 Robbauera albescens Species 0.000 claims description 4
- 238000011161 development Methods 0.000 claims description 4
- 230000018109 developmental process Effects 0.000 claims description 4
- 238000004382 potting Methods 0.000 claims description 4
- 241000257303 Hymenoptera Species 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 claims description 3
- 230000012447 hatching Effects 0.000 claims description 3
- 230000014759 maintenance of location Effects 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 2
- 230000013011 mating Effects 0.000 claims 4
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 208000007984 Female Infertility Diseases 0.000 claims 1
- 206010021928 Infertility female Diseases 0.000 claims 1
- 235000019504 cigarettes Nutrition 0.000 claims 1
- 241000607479 Yersinia pestis Species 0.000 abstract description 12
- 230000002265 prevention Effects 0.000 abstract description 4
- 240000002395 Euphorbia pulcherrima Species 0.000 abstract 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract 1
- 240000003768 Solanum lycopersicum Species 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000004321 preservation Methods 0.000 abstract 1
- 241000254127 Bemisia tabaci Species 0.000 description 18
- 241000208125 Nicotiana Species 0.000 description 8
- 241000219146 Gossypium Species 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000005286 illumination Methods 0.000 description 3
- 238000011031 large-scale manufacturing process Methods 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- 241000254123 Bemisia Species 0.000 description 2
- 241001481304 Vespoidea Species 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 241000353216 Aphelinus Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000241602 Gossypianthus Species 0.000 description 1
- 206010061217 Infestation Diseases 0.000 description 1
- 241001674048 Phthiraptera Species 0.000 description 1
- 241000018137 Trialeurodes vaporariorum Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000192 social effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention discloses a kind of biological control methods of aleyrodid pest obligatory parasitism bee-bis- spots grace aphid chalcid fly, by being completed in independent greenhouse in cleaning planting room, aleyrodid transfer room, aleyrodid breeding room, double spot grace aphid chalcid fly transfer rooms and five, separation chamber of aleyrodid-Shuan Banenya chalcid flies.The method is followed successively by:The plantation of host plant tobacco, cotton, tomato, cabbage mustard and poinsettia is cultivated;Different host plant aleyrodid inoculations;Aleyrodid develops on different host plants, the inoculation of double spot grace aphid chalcid flies, the bis- spot grace aphid chalcid flies separation of aleyrodid-, bee pupa is collected, pupa box makes preservation etc..It may be implemented the biological control of double spot grace aphid chalcid flies by above technical scheme, more host plants cultivate the adaptability to host when can increase the practical prevention aleyrodid of double spot grace aphid chalcid flies, to improve its parasitism efficiency.
Description
Technical field
The present invention relates to agriculture and forestry biological control of insect pests fields, more particularly, to a kind of scale of double spot grace aphid chalcid flies
Change propagation method.
Background technology
In recent years, with global warming, the generation and harm of the aleyrodids pest such as Bemisia tabaci and Trialeurodes vaporariorum Westwood are year by year
It aggravates, since it is extensive with host range, the features such as development of drug resistance is rapid, serious commercial is caused to crops and flowers
Loss.Currently, pesticide control is still to control the main means of aleyrodid class pest, but the use of pesticide not only has residual, dirt
The problems such as dye environment, pest resistance to insecticide increase can also kill natural enemy insect.Therefore, the biological preventing control method of aleyrodid becomes and currently grinds
Study carefully the hot spot with application.
Double spot grace Aphelinus Hymenoptera aphid Chalcididaes are one of the advantage parasitic wasps of South China's aleyrodid class pest,
It plays an important role, has a good application prospect to the prevention and control of aleyrodid class pest in nature.It is small with double spot grace aphids
Bee prevents the green bio Prevention Technique of aleyrodid class pest, is gradually concerned by people.But up to the present, not yet
There is the research of the large-scale production of double spot grace aphid chalcid flies so that double spot grace aphid chalcid flies receive the control action of aleyrodid class pest
Greatly limit.Therefore, double spot grace aphid chalcid fly large-scale production technologies are studied, the biological control application of aleyrodid class pest is carried out,
With good economic, ecology and social effect.
Invention content
The technical problem to be solved by the present invention is to supplement the blank of double spot grace aphid chalcid fly reproduction techniques, a kind of double spots are provided
The method of grace aphid chalcid fly biological control.
The above-mentioned purpose of the present invention is to give realization by the following technical programs:
A kind of biological control method of double spot grace aphid chalcid flies, includes the following steps:
S1. host plant is planted:Plantation is for different types of host plant needed for aleyrodid growth reproduction;
S2. aleyrodid is inoculated with:Different types of host plant that S1 grows fine is respectively put into the insect cage of aleyrodid transfer room,
The aleyrodid of spawning period is inoculated on host plant by blade cage again, controls every host plant blades adult whitefly quantity
It it is 80~100, after allowing its breeding to lay eggs 24 hours, with 30~40 ovum/cm2It is advisable, then catches up with all adult whiteflies
It walks, 22~28 DEG C of room temperature of control inoculation, humidity 60%~80%;
S3. aleyrodid breeds:The host plant that S2 has been inoculated with to aleyrodid ovum moves in aleyrodid breeding room and uniformly places, and allows aleyrodid ovum
Hatching development;If there is residual adult whitefly on host plant, DDVP paper slip can be used 4 hours stifling, kill the aleyrodid left
Adult;22~28 DEG C of room temperature of control breeding, humidity 60%~80%, wait for that vacuum side of blade aleyrodid nymph quantity was developed to for 2~3 ages
When, it can be used for the inoculation of aphid chalcid fly;
S4. double spot grace aphid chalcid fly queen bee breedings:The host plant of 2~3 age aleyrodid nymph of band in S3 is moved into double spot grace aphid chalcid flies
Transfer room, by bee:Aleyrodid 1:The 20 double spot grace aphid chalcid fly queen bees of access post-coitum, remove bee after 2~3 days, connect bee indoor temperature and exist
22~28 DEG C, humidity 60%~80% can be obtained the female pupa of double spot aphid chalcid flies after 10~11 days;
S5. double spot grace aphid chalcid fly drone breedings:It will be carried by the host plant of 3 age aleyrodid nymphs of double spot aphid chalcid flies parasitism in S4
Double spot grace aphid chalcid fly drone breeding rooms are moved to, statistics is by the quantity of parasitic aleyrodid nymph, according to by parasitic aleyrodid nymph:Double spots
Grace aphid chalcid fly=5:1 ratio accesses the latter, allows its hyperparasite previously by parasitic aleyrodid nymph, obtains in this manner double
Spot grace aphid chalcid fly drone;Control connects 22~28 DEG C of bee indoor temperature, humidity 60%~80%, and double spot grace are can be obtained after 10~11 days
Aphid chalcid fly hero pupa;
S6. double spot grace aphid chalcid fly inoculation breedings:The host plant that 2~3 age aleyrodid nymphs are carried in S3 is uniformly placed on parasitism
In bee transfer room, by parasitic wasp of the emergence time in S4, S5 within 24 hours by female:Male=2:1 carries out population in advance mixes
It closes, allows double spot grace aphid chalcid fly pangamies 24~48 hours;Then according to aleyrodid:Parasitic wasp=20:Aphid chalcid fly is accessed blade by 1
Cage, allows double spot grace aphid chalcid fly parasitism aleyrodid nymphs, and parasitic wasp is removed after 4~5 days;Control connects bee breeding indoor temperature 22~28
DEG C, humidity 60%~80%;
S7. aleyrodid-aphid chalcid fly separation chamber:It is even at this time to have not by the aleyrodid of double spot grace aphid chalcid fly parasitisms plumage after 8~12 days
Adult is turned to, is left puppet puparium, plant can be moved to aleyrodid-aphid chalcid fly separation chamber at this time, host plant blades can be picked, dried in the shade
Bee box is made afterwards, or the small bee pupa card of double spot grace aphids is made after washing away with water, is placed in 8~10 DEG C of refrigerator storages.
The host plant that the propagation method of the present invention uses it is numerous in variety, mixing raising aleyrodid then raise pair spot grace aphids
Chalcid fly can improve host's adaptability of parasitic wasp, to improve the parasitic rate in its application process.In addition, host plant leaf
Piece is more, big, blade face is smooth, is conducive to aleyrodid and expands numerous and bee box to make preferably, host plant described in S1 be tobacco, cotton,
At least two in cabbage mustard and poinsettia.
Preferably, described tobacco 8 or more true leaf, wait for that 6, cotton or more true leaf, wild cabbage 6 or more true leaf, poinsettia are cut
It is spare after spraying DDVP desinsection one month except old leaf.
Preferably, host plant described in S1 is all made of potting mode and plants, convenient for management.
Preferably, in host plant plantation room, aleyrodid transfer room, aleyrodid breeding room, double spot grace aphid chalcid flies inoculation breeding room
The distance between every potted plant interval is 20~30 centimetres.
Preferably, host plant plantation room, aleyrodid transfer room, aleyrodid breeding room, the inoculation breeding of double spot grace aphid chalcid flies
Room, aleyrodid-five, aphid chalcid fly separation chamber greenhouse are not interfere with each other separately independently, and indoor conditions is controlled at 22~28 DEG C(It is preferred that
26℃), RH60%~80%(It is preferred that 65%), illumination shading value L:D=14:10.
Compared with existing other aphid chalcid fly reproduction techniques, the invention has the advantages that:
(1)Double spot grace aphid chalcid flies are the advantage parasitic wasps of South China aleyrodid pest, to powder in protecting field or industrialized agriculture
The biological control significant effect of lice pest.
(2)The large-scale breeding method of double spot grace aphid chalcid flies of the present invention, it is various using host plant, resist other insects
And the ability of disease infestation is stronger than single host plant, meanwhile, a variety of host plant raising aleyrodids then raise parasitic wasp, can
To improve host's adaptability of parasitic wasp, the parasitic rate in its actual application is improved.
(3)The large-scale breeding method of double spot grace aphid chalcid flies of the present invention, method is simple, easily grasps, it can be achieved that double spot grace
The whole year uninterrupted breeding of aphid chalcid fly, though not the keeper of professional knowledge if can learn in a short time;Feeding cost
It is cheap, after large-scale production obtains putting bee box or putting bee card for field prevention and control aleyrodid, huge society, ecology can be brought
And economic benefit.
Specific implementation mode
Further illustrated the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagent, methods and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are purchased in market.
Embodiment 1
(1)Facility prepares
Build host plant plantation room, numerous room is expanded in aleyrodid transfer room, aleyrodid breeding room, the inoculation of double spot grace aphid chalcid flies, aleyrodid-aphid is small
Bee separation chamber:Area depends on the needs, general 30~50m2, exhaust fan and 1.5 air conditioner control temperature are respectively mounted, and
The foster worm frame of different number is put according to size, and time switch control is installed and supports worm bracket lamp pipe switch time.
Support worm frame:The foster worm frame of 165cm*130cm*75cm several, each shelf point two layers, every layer of top installation 6
The fluorescent lamp of 28w each supports every layer of worm frame and installs a manual switch respectively.
(2)Rearing conditions
Temperature, humidity and illumination condition:Host plant culturing room, aleyrodid transfer room, aleyrodid expand numerous room, parasitic wasp transfer room and
The indoor conditions for expanding numerous room is controlled at 22~28 DEG C(26 DEG C best), RH60%~80%(65% is best), illumination shading value L:
D=14:10。
(3)Worm sources prepare
Bemisia tabaci:Worm sources come from engineering center of the biological control Ministry of Education(Agricultural University Of South China)
Double spot grace aphid chalcid flies:Worm sources come from engineering center of the biological control Ministry of Education(Agricultural University Of South China)
(4)Host plant is cultivated
Tobacco coating seed(PVH19)It is purchased from Weifang City of Shandong Province kind for the production of Yun Xizhongyan seeds Co., Ltd, cotton
Sub- management station, wild cabbage are purchased from seed control station of Weifang City of Shandong Province, poinsettia potting is purchased from the villages Fo Shanchen flowers market, by cotton
Flower, tobacco and wild cabbage carry out nursery, and the seedling brought out is transplanted to by batch in plant culturing room and carries out potting, by poinsettia into
Row, which wipes out old leaf and sprays DDVP, carries out desinsection processing, waits for tobacco 8 or more true leaf, waits for 6, cotton or more true leaf, wild cabbage 6
The above true leaf, poinsettia spray are spare when placing one month or more.Tobacco, cotton and cabbage mustard were transplanted in transplanting every 20 days
Once, poinsettia is periodically bought, it is ensured that to have host plant spare throughout the year.
(5)Bemisia tabaci is inoculated with
It is respectively put into step in the insect cage of Bemisia tabaci transfer room(4)The different types of host plant to grow fine, then lead to
It crosses blade cage the Bemisia tabaci of spawning period is inoculated on host plant, every host plant blades adult whitefly quantity of control is
After 80~100 allow its breeding to lay eggs 24 hours, with 30-40 ovum/cm2It is advisable, then catches up with all Bemisia tabaci adults
It walks., inoculation room temperature control is in 22~28 DEG C, humidity 60%~80%.
(6)Bemisia tabaci is bred
By step(5)The host plant for being inoculated with tobacco powder nit moves in Bemisia tabaci breeding room and uniformly places, and allows tobacco powder nit
Hatching development.If checking on host plant there are residual Bemisia tabaci adults, DDVP paper slip can be used 4 hours stifling, kill is left
Bemisia tabaci adults.Room temperature control is bred in 22~28 DEG C, humidity 60%~80%.Vacuum side of blade Bemisia tabaci Nymph quantity to be made
When being developed to for 2~3 age, it can be used for double spot grace aphid chalcid fly inoculations.
(7)Double spot grace aphid chalcid fly queen bee breedings
By step(6)The host plant of middle band 2~3 age aleyrodid nymph moves to double spot grace aphid chalcid fly transfer rooms, by bee:Aleyrodid 1:20
Access the double spot grace aphid chalcid fly queen bees of post-coitum, bee removed after 2~3 days, connect bee indoor temperature 22~28 DEG C, humidity 60%~
It can be obtained the female pupa of double spot aphid chalcid flies after 80%, 10~11 days.
(8)Double spot grace aphid chalcid fly drone breedings
By step(7)In with moving to pair spot grace aphids by the host plant of the 2-3 age Bemisia tabaci Nymphs of double spot grace aphid chalcid fly parasitism
Chalcid fly drone breeds room, and statistics is by the quantity of parasitic Bemisia tabaci Nymph, according to by parasitic Bemisia tabaci Nymph:Double spot grace aphid chalcid flies
=5:1 ratio accesses the latter, allows its hyperparasite previously by parasitic Bemisia tabaci Nymph, obtains double spot grace aphids in this manner
Chalcid fly drone.22~28 DEG C of bee indoor temperature, humidity 60%~80% are connect, it is male that double spot grace aphid chalcid flies are can be obtained after 10~11 days
Pupa.
(9)Double spot grace aphid chalcid fly inoculation breedings:By step(6)In uniformly put with the host plants of 2-3 age aleyrodid nymphs
It sets in parasitic wasp transfer room, by step(7),(8)In parasitic wasp of the emergence time within 24 hours by female:Male=2:1 is pre-
The mixing of first Advanced group species, allows double spot grace aphid chalcid fly pangamies 24~48 hours.Then according to Bemisia tabaci:Parasitic wasp=20:1 will
Aphid chalcid fly accesses blade cage, allows double spot grace aphid chalcid fly parasitism Bemisia tabaci Nymphs, and parasitic wasp is removed after 4~5 days.It is indoor to connect bee breeding
22~28 DEG C of temperature, humidity 60%~80%.
(10)Bemisia tabaci-Shuan Banenya chalcid flies separation chamber
After about 10 days, occasionally have adult has not been turned by the Bemisia tabaci of double spot grace aphid chalcid fly parasitisms at this time, is left puppet puparium.This
When plant can be moved to Bemisia tabaci-Shuan Banenya chalcid flies separation chamber, host plant blades can be picked, after drying in the shade make bee box, or
The small bee pupa card of double spot grace aphids is made after washing away with water, is placed in 8~10 DEG C of refrigerator storages.
Claims (5)
1. a kind of biological control method of double spot grace aphid chalcid flies, which is characterized in that include the following steps:
S1. host plant is planted:Plantation is for different types of host plant needed for aleyrodid growth reproduction;
S2. aleyrodid is inoculated with:Different types of host plant that S1 grows fine is respectively put into the insect cage of aleyrodid transfer room,
The aleyrodid of spawning period is inoculated on host plant by blade cage again, controls every host plant blades adult whitefly quantity
It it is 80~100, after allowing its breeding to lay eggs 24 hours, with 30~40 ovum/cm2It is advisable, then catches up with all adult whiteflies
It walks, 22~28 DEG C of room temperature of control inoculation, humidity 60%~80%;
S3. aleyrodid breeds:The host plant that S2 has been inoculated with to aleyrodid ovum moves in aleyrodid breeding room and uniformly places, and allows aleyrodid ovum
Hatching development;If there is residual adult whitefly on host plant, DDVP paper slip can be used 4 hours stifling, kill the aleyrodid left
Adult;22~28 DEG C of room temperature of control breeding, humidity 60%~80%, wait for that vacuum side of blade aleyrodid nymph quantity was developed to for 2~3 ages
When, it can be used for the inoculation of aphid chalcid fly;
S4. double spot grace aphid chalcid fly queen bee breedings:The host plant of 2~3 age aleyrodid nymph of band in S3 is moved into double spot grace aphid chalcid flies
Transfer room, by bee:Aleyrodid 1:The 20 double spot grace aphid chalcid fly queen bees of access post-coitum, remove bee after 2~3 days, connect bee indoor temperature and exist
22~28 DEG C, humidity 60%~80% can be obtained the female pupa of double spot aphid chalcid flies after 10~11 days;
S5. double spot grace aphid chalcid fly drone breedings:It will be carried by the host plant of 3 age aleyrodid nymphs of double spot aphid chalcid flies parasitism in S4
Double spot grace aphid chalcid fly drone breeding rooms are moved to, statistics is by the quantity of parasitic aleyrodid nymph, according to by parasitic aleyrodid nymph:Double spots
Grace aphid chalcid fly=5:1 ratio accesses the latter, allows its hyperparasite previously by parasitic aleyrodid nymph, obtains in this manner double
Spot grace aphid chalcid fly drone;Control connects 22~28 DEG C of bee indoor temperature, humidity 60%~80%, and double spot grace are can be obtained after 10~11 days
Aphid chalcid fly hero pupa;
S6. double spot grace aphid chalcid fly inoculation breedings:The host plant that 2~3 age aleyrodid nymphs are carried in S3 is uniformly placed on parasitism
In bee transfer room, by parasitic wasp of the emergence time in S4, S5 within 24 hours by female:Male=2:1 carries out population in advance mixes
It closes, allows double spot grace aphid chalcid fly pangamies 24~48 hours;Then according to aleyrodid:Parasitic wasp=20:Aphid chalcid fly is accessed blade by 1
Cage, allows double spot grace aphid chalcid fly parasitism aleyrodid nymphs, and parasitic wasp is removed after 4~5 days;Control connects bee breeding indoor temperature 22~28
DEG C, humidity 60%~80%;
S7. aleyrodid-aphid chalcid fly separation chamber:After 8~12 days, occasionally has do not sprouted wings by the aleyrodid of double spot grace aphid chalcid fly parasitisms at this time
For adult, it is left puppet puparium, plant can be moved to aleyrodid-aphid chalcid fly separation chamber at this time, host plant blades can be picked, after drying in the shade
Bee box is made, or the small bee pupa card of double spot grace aphids is made after washing away with water, is placed in 8~10 DEG C of refrigerator storages.
2. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that host plant described in S1 is cigarette
At least two in grass, cotton, cabbage mustard and poinsettia.
3. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that host plant described in S1 is adopted
It is planted with potting mode.
4. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that described tobacco 8 or more is true
It is leaf, spare after 6, cotton or more true leaf, wild cabbage 6 or more true leaf, poinsettia wipe out old leaf spray DDVP desinsection one month.
5. the mating system of double spot grace aphid chalcid flies according to claim 1, which is characterized in that double spot grace aphid chalcid flies are
Lonely female infertility, both sexes produce female mode of reproduction, and drone need to be of the same race through hyperparasite or belongs to the acquisition of sibling species aphid chalcid fly.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810411727.7A CN108739675B (en) | 2018-05-02 | 2018-05-02 | Large-scale breeding method for Encarsia bimaculata |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810411727.7A CN108739675B (en) | 2018-05-02 | 2018-05-02 | Large-scale breeding method for Encarsia bimaculata |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108739675A true CN108739675A (en) | 2018-11-06 |
CN108739675B CN108739675B (en) | 2021-03-30 |
Family
ID=64008906
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810411727.7A Active CN108739675B (en) | 2018-05-02 | 2018-05-02 | Large-scale breeding method for Encarsia bimaculata |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108739675B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110338154A (en) * | 2019-08-09 | 2019-10-18 | 河南科技大学 | For breeding vector plant system and construction method, the propagation method and the method for preventing and treating aleyrodid class pest of pale yellow grace aphid chalcid fly |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101390505A (en) * | 2008-10-28 | 2009-03-25 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
KR20120071946A (en) * | 2010-12-23 | 2012-07-03 | 대한민국(농촌진흥청장) | Mass rearing of geocoris pallidipennis |
US20150030689A1 (en) * | 2011-09-20 | 2015-01-29 | Koppert B.V. | Phytoseiid predatory mite releasing system and method for production |
CN107396891A (en) * | 2017-08-22 | 2017-11-28 | 中国农业科学院植物保护研究所 | A kind of method that constant low temperature stores the pale yellow female pupa of grace aphid chalcid fly |
-
2018
- 2018-05-02 CN CN201810411727.7A patent/CN108739675B/en active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101390505A (en) * | 2008-10-28 | 2009-03-25 | 北京市农林科学院 | Encarsia Sophia artificial propagation production method |
KR20120071946A (en) * | 2010-12-23 | 2012-07-03 | 대한민국(농촌진흥청장) | Mass rearing of geocoris pallidipennis |
US20150030689A1 (en) * | 2011-09-20 | 2015-01-29 | Koppert B.V. | Phytoseiid predatory mite releasing system and method for production |
CN107396891A (en) * | 2017-08-22 | 2017-11-28 | 中国农业科学院植物保护研究所 | A kind of method that constant low temperature stores the pale yellow female pupa of grace aphid chalcid fly |
Non-Patent Citations (1)
Title |
---|
钱明惠等: "双斑恩蚜小蜂的生殖方式及其在烟粉虱体内的发育", 《应用昆虫学报》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110338154A (en) * | 2019-08-09 | 2019-10-18 | 河南科技大学 | For breeding vector plant system and construction method, the propagation method and the method for preventing and treating aleyrodid class pest of pale yellow grace aphid chalcid fly |
Also Published As
Publication number | Publication date |
---|---|
CN108739675B (en) | 2021-03-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN103385220B (en) | Scale breeding method for encarsia formosa | |
CN102119660B (en) | Method for rooting culture,seedling adaptation and transplantation of alpine rose tissue-cultured seedling in greenhouse | |
CN102172231B (en) | Method for overwintering species conservation propagation of myzus persicae | |
CN105684738A (en) | Sunlight greenhouse tomato culture method | |
CN105191763A (en) | Vegetable organic soil-less culture method, culture medium and manufacturing method thereof | |
CN104585135A (en) | Overwintering breeding method for aphidiusgifuensis in northern area | |
CN103960203B (en) | A kind of Hai Shi of utilization eretmocerus SP breeds the method for pale yellow grace aphid chalcid fly Xiong Feng | |
CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
CN101715754A (en) | Method for raising small brown rice planthopper by utilizing soil-less cultured barley seedling | |
CN106900656B (en) | A kind of cigarette fleahopper industrial production method | |
CN106689066A (en) | Method for quickly breeding aphidius gifuensis | |
CN101019522A (en) | Large scale artificial spotted butterfly cultivating technology | |
CN105340836A (en) | Method for improving factory propagation efficiency of encarsia formosa | |
CN106172248A (en) | A kind of expanding propagation method of Japan food a red-spotted lizard aphid chalcid fly | |
CN104604809A (en) | Biological control method using encarsia formosa for controlling tobacco whitefly | |
CN107787698A (en) | A kind of production method for the seed potato for avoiding soil-borne disease from infecting | |
CN104756955B (en) | Aphidius gifuensis are with numerous method together with Encarsia formosa | |
CN108739675A (en) | A kind of method of double spot grace aphid chalcid fly biological controls | |
CN103210881A (en) | Large-scale artificial breeding method for Neoseiulu californicus | |
CN113558014B (en) | Large-scale production method of dormitory lice flea bees | |
CN112470834B (en) | High-yield planting method for cherry tomatoes | |
CN101019523A (en) | Large scale artificial white butterfly cultivating technology | |
CN107517715A (en) | A kind of implantation methods of the stem of noble dendrobium | |
CN113951023A (en) | Method for preventing and treating mango thrips by utilizing orius minutus | |
CN113661964A (en) | Method for indoor breeding tea lesser leafhoppers |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |