CN103168590A - Bletilla striata seed propagating method - Google Patents
Bletilla striata seed propagating method Download PDFInfo
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- CN103168590A CN103168590A CN2013100851095A CN201310085109A CN103168590A CN 103168590 A CN103168590 A CN 103168590A CN 2013100851095 A CN2013100851095 A CN 2013100851095A CN 201310085109 A CN201310085109 A CN 201310085109A CN 103168590 A CN103168590 A CN 103168590A
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- 241001313857 Bletilla striata Species 0.000 title abstract 5
- 230000001902 propagating effect Effects 0.000 title abstract 3
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- 235000015097 nutrients Nutrition 0.000 claims abstract description 10
- 238000009333 weeding Methods 0.000 claims abstract description 4
- 241001313855 Bletilla Species 0.000 claims description 53
- 239000012531 culture fluid Substances 0.000 claims description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000007921 spray Substances 0.000 claims description 6
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- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 claims description 4
- 150000005018 aminopurines Chemical class 0.000 claims description 4
- 235000016709 nutrition Nutrition 0.000 claims description 4
- 230000035764 nutrition Effects 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 3
- 235000019362 perlite Nutrition 0.000 claims description 3
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- 235000019354 vermiculite Nutrition 0.000 claims description 3
- 229910052902 vermiculite Inorganic materials 0.000 claims description 3
- 238000012423 maintenance Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000002686 phosphate fertilizer Substances 0.000 claims description 2
- 239000002985 plastic film Substances 0.000 claims description 2
- 229920006255 plastic film Polymers 0.000 claims description 2
- 239000003337 fertilizer Substances 0.000 abstract description 2
- 239000004033 plastic Substances 0.000 abstract description 2
- 238000005507 spraying Methods 0.000 abstract 1
- 239000010409 thin film Substances 0.000 abstract 1
- 239000012452 mother liquor Substances 0.000 description 12
- 239000000243 solution Substances 0.000 description 9
- 239000002775 capsule Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 4
- 238000009395 breeding Methods 0.000 description 4
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- 238000011160 research Methods 0.000 description 4
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- 235000013343 vitamin Nutrition 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 150000003722 vitamin derivatives Chemical class 0.000 description 4
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 3
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- 238000009331 sowing Methods 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 159000000007 calcium salts Chemical class 0.000 description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-N calcium;phosphoric acid Chemical compound [Ca+2].OP(O)(O)=O.OP(O)(O)=O YYRMJZQKEFZXMX-UHFFFAOYSA-N 0.000 description 2
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- 239000002426 superphosphate Substances 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
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Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention provides a bletilla striata seed propagating method. The method includes the steps: (1) enabling collected bletilla striata seeds to be sown to appropriate soil media, and pouring appropriate 1/2 MS sugar-free nutrient solution to the seeds; (2) after the seeds are sown, controlling the day growth temperature to be 15-25 DEG C and controlling relative air humidity to be kept above 70%, using a plastic thin film to cover the seeds and arranging a sunshade net above to overshadow the seeds; (3) after the seeds are sown for one week, removing the sunshade net, and spraying a nutrient solution at a fixed time and watering for keeping moisture timely; (4) after the seeds are sown for one month, spreading appropriate phosphatic fertilizer, and watering and weeding timely, and conducting transplanting until corms grow from underground. The bletilla striata seed propagating method can be adopted to enable seedlings of bletilla striata to largely propagate within a short time, and effectively meets the demands of planting industry to the seedlings.
Description
(1) technical field
The present invention relates to a kind of propagation method of bletilla seed.
(2) background technology
Bletilla is the dry tuber of orchid bletilla (Bletilla sfriata (Thunb.) Reiehb.f.), in most wild endroit in hill Chuan Gu, sparse woods, in thick grass, like the shady and cool larger environment of humidity, fertile soil loosens and well-drained folder sandy soil.So the resource that requires the bletilla of living environment uniqueness is limited, at present, the place of production of wild resource is the remote hill of the extremely limited minority region such as remaining cloud, expensive, river, Hunan only.
Bletilla is perennial herb, plant short and small (without the wild germplasm resource of artificially breeding), and pale reddish brown bletilla high 30 centimetres of left and right, it is high more than 33 centimetres that Emei, Sichuan Province is spent Herba Phaii tankervilliaes in vain.Bletilla leaf 3-6 sheet, long lanceolar, the middle part is slightly wide, the tip point.Bletilla is sprouted March, the florescence 4-5 month, the fruit phase 8-10 month.Fall seedling December.Although the bletilla result,, but can not rely on the seed procreation.Ripe bletilla seed is olive-type, and yellow powder shape is very tiny, and planting skin is that cell monolayer forms, the sidewall thickening, and organelle and protoplasm all disappear, are the translucent dead cells of one deck, the embryo ellipse, differentiation, be not the proembryo stage, suspensor is degenerated, without endosperm.Under nature, few Germination And Seedling.At present, the bletilla main stem tuber that relies on is at present bred, and is typical 1+1=2 modes of reproduction.Long-term vegetative propagation, not only reproduction speed is slow, and large with consumption kind of amount, and also with medication contention raw material, long-term vegetative propagation easily causes kind of a sexual involution, is difficult to adapt to the needs of large-scale planting.And soil farming year after year, the bletilla bulb has accumulated a large amount of viruses, has had a strong impact on its output and kind.
For this reason, there is the scholar to attempt the mode of cultivating by tissue, hangs down to solve the bletilla reproductive efficiency problems such as reaching virus infections, and obtained encouraging success.Yet, sum up bletilla tissue-culturing rapid propagation production technology, have following problem: bletilla bulb, stem section contain a large amount of endogenetic fungus and virus, and group training pollution rate is high, and success rate is low; Although utilize bletilla shoot tip meristem and seed group training success rate high, but from dedifferentiation, propagation, differentiation, hardening until the transplanting cycle long, about at least 5 months (the orchid growth is slowly general), and during to change medium several times, workload is large, cost and with high content of technology, general enterprises and self-employed worker medicinal herb grower do not accomplish at all; For this reason, still do not have essence to solve the problem of bletilla, to present up-trend be perhaps to be also preferably the most directly to confirm to the bletilla price always so far.For this reason, still be necessary to seek better, more economical breeding and planting technology method easily, expanding production is to meet the need of market.
At present, the research work of bletilla artificial breeding progress is very slow, and this is the wild key subject that changes family's kind research of bletilla.The secret of this growth link has surmounted most producers' the level of understanding, and hence one can see that, at present the bletilla man plant can only be take pseudobulb as seedling, and only have then and give birth to, the pseudobulb with old bar and tender shoots just can develop into new plant.Bletilla development man plants, and must face the rare predicament of seed rice shoot.Moreover this traditional division propagation mode, inevitably yielding poorly down, diseases and insect pests resistance is poor, and a generation generation is degenerated.Because seedling is in short supply, extensive industrialization plantation is just present also out of reach.
(3) summary of the invention
The present invention is directed to the bletilla reproductive efficiency low, mostly rely on vegetative problem, the method for utilizing seminal propagation rapidly and efficiently to cultivate high quality seedling is provided.
The technical solution used in the present invention is:
A kind of propagation method of bletilla seed, described method comprises:
(1) the bletilla seed (gather annual 8～September) of collection is sowed to suitable soil media, and watered the appropriate 1/2MS sugar-free culture fluid of pouring; The bletilla seed is tiny of endosperm, so in the organic abundant soil such as need sowing and peat soil, Nutrition Soil; The bletilla seed needed sterile-processedly before sowing usually, can directly carry out disinfection to capsule, carry out disinfection after also can the seed in capsule taking out;
(2) after planting seed, controlling the daytime growth temperature is 15～25 ℃, and relative air humidity remains on more than 70%, and the top covers with plastic film, and sunshade net is set suitably shelters from heat or light;
(3) planting seed is removed sunshade net after one week, and the moistening (each 200～1500mL/m once in a week, of nutrient solution and the maintenance of in good time watering regularly sprays
2); Described nutrient solution is composed as follows: 6-benzyl aminopurine 0～2.0mg/L, 1-methyl α-naphthyl acetate 0～1.0mg/L, solvent are 1/2MS sugar-free culture fluid or MS sugar-free culture fluid;
(4) planting seed is executed appropriate phosphate fertilizer (as superphosphate, consumption is 5～15g/m after one month
2), and carry out according to a conventional method scientific management, (as spending precious No. 1, consumption is 0.2～0.8g/m in the weeding of in good time watering fertilising
2), until undergroundly transplant after growing bulb.
Preferably, described bletilla seed was first cultivated 1～2 month in the 1/2MS culture fluid that adds 0～2.0mg/L6-benayl aminopurine (be 0 and do not add, preferred 1.0mg/L) or MS culture fluid, and the bletilla seed after being sprouted is sowed to soil media again.
The described soil media of step (1) is peat soil.
The described soil media of step (1) is the mixture of Nutrition Soil, vermiculite, perlite mass ratio 20:1～5:1～5.
The described nutrient solution of step (3) is composed as follows: 6-benzyl aminopurine 1.0～2.0mg/L, 1-methyl α-naphthyl acetate 0.5～1.0mg/L, solvent are 1/2MS sugar-free culture fluid or MS sugar-free culture fluid;
In the present invention, MS culture fluid and MS sugar-free culture fluid formula examples are as follows:
Macroelement mother liquor: NH
4NO
316.5g; MgSO
47H
2O3.7g; KH
2PO
41.7g; KNO
319g is dissolved in water, and is settled to 500ml;
Calcisolution: CaCl
23.32g, be dissolved in water, be settled to 500ml;
Trace element mother liquor: KI0.0415g; Na
2MoO
42H
2O0.0125g; H
3BO
30.31g; CuSO
45H
2O0.00125g; MnSO
4H
2O0.845g; CoCl
26H
2O0.00125g; ZnSO
47H
2O0.43g is dissolved in water, and is settled to 500ml;
Mother liquid of iron salt: EDETATE SODIUM 3.725g adds 100ml water heating for dissolving, FeSO
47H
2O2.785g adds both mixings after the water-soluble solution of 100ml, adds water and is made into 500ml;
Vitamin mother liquor: puridoxine hydrochloride (VB6) 0.025g; Thiamine hydrochloride (VB1) 0.025g; Nicotinic acid 0.025g, glycine 0.1g is dissolved in water, and is settled to 500ml;
Join the 1LMS culture fluid: get macroelement mother liquor 50ml, Calcisolution 50ml, micro-mother liquor 10ml, vitamin mother liquor 10ml, mother liquid of iron salt 5ml adds inositol 0.1g, sucrose or white granulated sugar 20～30g, distilled water is settled to 1L.
Join 1LMS sugar-free culture fluid: get macroelement mother liquor 50ml, Calcisolution 50ml, micro-mother liquor 10ml, vitamin mother liquor 10ml, mother liquid of iron salt 5ml, add inositol 0.1g and join the 1LMS culture fluid: get macroelement mother liquor 50ml, Calcisolution 50ml, micro-mother liquor 10ml, vitamin mother liquor 10ml, mother liquid of iron salt 5ml adds inositol 0.1g, sucrose or white granulated sugar 20～30g.
The 1/2MS culture fluid refers to that macroelement contained in solution and calcium salt are half of MS culture fluid, and other are constant.
1/2MS sugar-free culture fluid refers to that macroelement contained in solution and calcium salt are half of MS sugar-free culture fluid, and other are constant.
Although the bletilla result,, but can not rely on the seed procreation.Ripe bletilla seed is olive-type, and yellow powder shape is very tiny, and planting skin is that cell monolayer forms, the sidewall thickening, and organelle and protoplasm all disappear, are the translucent dead cells of one deck, the embryo ellipse, differentiation, be not the proembryo stage, suspensor is degenerated, without endosperm.Under nature, few Germination And Seedling.At present, the bletilla main stem tuber that relies on is at present bred, and is typical 1+1=2 modes of reproduction.The research work progress of bletilla artificial breeding is very slow, and this is the wild key subject that changes family's kind research of bletilla.The secret of this growth link has surmounted most producers' the level of understanding, and hence one can see that, at present the bletilla man plant can only be take pseudobulb as seedling, and only have then and give birth to, the pseudobulb with old bar and tender shoots just can develop into new plant.Bletilla development man plants, and must face the rare predicament of seed rice shoot.Moreover this traditional division propagation mode, inevitably yielding poorly down, diseases and insect pests resistance is poor, and a generation generation is degenerated.Because seedling is in short supply, extensive industrialization plantation is just present also out of reach.Utilize the present invention, can obtain a large amount of bletilla high quality seedlings in one-year age.
Beneficial effect of the present invention is mainly reflected in: adopt the inventive method, can breed in a large number at short notice the seedling of bletilla, effectively satisfy plant husbandry to the demand of seedling.
(4) embodiment
The present invention is described further below in conjunction with specific embodiment, but protection scope of the present invention is not limited in this:
Embodiment 1:
1) gather ripe uncracked bletilla capsule in by the end of August;
2) capsule is cut, take out seed wherein, soaked two days in water, be seeded in peat soil, water permeable, the top with plastic mulching after lid layer sunshade net again, the environment of planting seed is artificial single booth, booth top sunshade net covers, and in booth, regularly spray water every day, keeps humidity more than 70%;
3) planting seed is removed the sunshade net of surface coverage after one week, and 1/2MS sugar-free culture fluid (each approximately 800mL/m sprays weekly one time
2), and water, avoid dry lack of water. in good time
4) planting seed is removed the film of surface coverage after one month, and waters superphosphate (the about 1000mL/m of 10g/L
2), to promote taking root of seedling.
5) carrying out after this management of science, is mainly the weeding of in good time watering, and sprays page fertilizer, (spend precious No. 1,1000 times of dilutions, every two weeks spray 1 time in leaves and stems, approximately 500ml/m
2), can get underground cormatose bletilla seedling after 1 year.
Embodiment 2:
Concrete grammar is with embodiment 1, as different from Example 1, step 2) the bletilla capsule in is first with cutting capsule after 75% alcohol and the sterilization of 0.1% mercuric chloride, get the bletilla aseptic seed, be seeded in the 1/2MS culture fluid and cultivate one and a half months, use filtered through gauze, get the bletilla seed after sprouting, then be seeded in peat soil.In step 3) nutrient solution used be 1/2MS sugar-free culture fluid+1.0mg/L1-methyl α-naphthyl acetate and+the 2.0mg/L6-benayl aminopurine;
According to the method described above, can get underground cormatose bletilla seedling after 1 year.
Embodiment 3:
Concrete grammar is with embodiment 2, and as different from Example 2, the nutrient solution of sowing during the bletilla seed germination is MS culture fluid+1.0mg/L6-benayl aminopurine.
According to the method described above, can get underground cormatose bletilla seedling after 1 year.
Embodiment 4:
Concrete grammar is with embodiment 2, as different from Example 2, step 2) in be seeded into Nutrition Soil after seed germination: vermiculite: perlite is 20:1:1.
According to the method described above, can get underground cormatose bletilla seedling after 1 year.
At last; note also that; above examples of implementation are only several specific embodiment of the present invention; the invention is not restricted to the embodiments described; also have many distortion; protection scope of the present invention is all thought in all distortion that personnel can directly derive from content of the present invention or association goes out that this area is relevant.
Claims (5)
1. the propagation method of a bletilla seed, described method comprises:
(1) with the bletilla planting seed that gathers to suitable soil media, and water and drench appropriate 1/2MS sugar-free culture fluid;
(2) after planting seed, controlling the daytime growth temperature is 15～25 ℃, and relative air humidity remains on more than 70%, and the top covers with plastic film, and sunshade net is set shelters from heat or light;
(3) planting seed is removed sunshade net after one week, and regularly spray nutrient solution and the maintenance of in good time watering are moistening; Described nutrient solution is composed as follows: 6-benzyl aminopurine 0～2.0mg/L, 1-methyl α-naphthyl acetate 0～1.0mg/L, solvent are 1/2MS or MS sugar-free culture fluid;
(4) planting seed is executed appropriate phosphate fertilizer after one month, and the weeding fertilising of in good time watering, until undergroundly transplant after growing bulb.
2. the method for claim 1 is characterized in that described bletilla seed first cultivated 1～2 month in the 1/2MS that adds 0～2.0mg/L6-benayl aminopurine or MS culture fluid, the bletilla seed after being sprouted is sowed to soil media again.
3. the method for claim 1, is characterized in that the described soil media of step (1) is peat soil.
4. the method for claim 1, is characterized in that the described soil media of step (1) is the mixture of Nutrition Soil, vermiculite, perlite mass ratio 20:1～5:1～5.
5. the method for claim 1, it is characterized in that the described nutrient solution of step (3) is composed as follows: 6-benzyl aminopurine 1.0～2.0mg/L, 1-methyl α-naphthyl acetate 0.5～1.0mg/L, solvent are 1/2MS or MS sugar-free culture fluid.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103404266A (en) * | 2013-08-05 | 2013-11-27 | 陕西师范大学 | Propagation method of bletilla striata |
| CN103444394A (en) * | 2013-08-15 | 2013-12-18 | 李子辉 | Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition |
| CN103875400A (en) * | 2014-03-14 | 2014-06-25 | 云南云蕊生物资源开发有限公司 | Method for accelerating growth and development of rhizoma bletillae |
| CN103947425A (en) * | 2014-04-28 | 2014-07-30 | 黔西南州凌飞中草药开发有限公司 | Rhizoma bletillae culture and planting method |
| CN106258903A (en) * | 2016-08-29 | 2017-01-04 | 浙江金石生物科技有限公司 | A kind of method of large-scale production Pseudobulbus Bletillae (Rhizoma Bletillae) seedling |
| CN106358823A (en) * | 2016-08-29 | 2017-02-01 | 遵义联谷农业科技有限公司 | Bletilla striata culture medium and preparation method thereof |
| CN106538208A (en) * | 2016-11-04 | 2017-03-29 | 贵州浩宇生物科技开发有限公司 | A kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery |
| CN107223444A (en) * | 2017-07-18 | 2017-10-03 | 四川康养健生物科技有限公司 | A kind of method that bletilla seed sowing is bred |
| CN108094105A (en) * | 2018-01-21 | 2018-06-01 | 大姚县春苗中药材种植有限公司 | The cultivation implantation methods of the bletilla striata |
| CN108935051A (en) * | 2018-09-26 | 2018-12-07 | 河南云帮农业科技有限公司 | A kind of bletilla striata live streaming seedling point cultivation field planting management method |
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| CN103444394A (en) * | 2013-08-15 | 2013-12-18 | 李子辉 | Method for realizing sexual reproduction and seedling culture of bletilla under imitated ecological condition |
| CN103875400A (en) * | 2014-03-14 | 2014-06-25 | 云南云蕊生物资源开发有限公司 | Method for accelerating growth and development of rhizoma bletillae |
| CN103947425A (en) * | 2014-04-28 | 2014-07-30 | 黔西南州凌飞中草药开发有限公司 | Rhizoma bletillae culture and planting method |
| CN106258903A (en) * | 2016-08-29 | 2017-01-04 | 浙江金石生物科技有限公司 | A kind of method of large-scale production Pseudobulbus Bletillae (Rhizoma Bletillae) seedling |
| CN106358823A (en) * | 2016-08-29 | 2017-02-01 | 遵义联谷农业科技有限公司 | Bletilla striata culture medium and preparation method thereof |
| CN106538208A (en) * | 2016-11-04 | 2017-03-29 | 贵州浩宇生物科技开发有限公司 | A kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery |
| CN107223444A (en) * | 2017-07-18 | 2017-10-03 | 四川康养健生物科技有限公司 | A kind of method that bletilla seed sowing is bred |
| CN108094105A (en) * | 2018-01-21 | 2018-06-01 | 大姚县春苗中药材种植有限公司 | The cultivation implantation methods of the bletilla striata |
| CN108935051A (en) * | 2018-09-26 | 2018-12-07 | 河南云帮农业科技有限公司 | A kind of bletilla striata live streaming seedling point cultivation field planting management method |
| CN114431110A (en) * | 2022-02-07 | 2022-05-06 | 中国科学院武汉植物园 | Bletilla striata seed culture medium combination and method for culturing bletilla striata seeds by using bletilla striata seed culture medium combination |
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