CN106538208A - A kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery - Google Patents
A kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery Download PDFInfo
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- CN106538208A CN106538208A CN201610965616.1A CN201610965616A CN106538208A CN 106538208 A CN106538208 A CN 106538208A CN 201610965616 A CN201610965616 A CN 201610965616A CN 106538208 A CN106538208 A CN 106538208A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
Abstract
The invention discloses a kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery, comprises the following steps：Get the raw materials ready：The preparation of a, substrate；B, seed sprout the preparation of nutritional solution：High potassium special fertilizer of total nutrient content more than 60% is configured, special fertilizer is configured to into 1000 times of liquid, and is added the naphthalene acetic acid of 0.5mg/L；Selection of land bedding；Furrow upper berth substrate：The substrate for preparing is laid on furrow, thickness is formed in the seedbed of 8cm；Sowing；Spray nutritious liquor；Do shed；Temperature control moisturizing；Spray foliage fertilizer；Remove plastic sheeting；Transplant.The present invention compared with prior art, sprouts nutritional solution by scientific and reasonable configuration seedling medium and seed, live in big Tanaka, it is ensured that the nutrition required for Pseudobulbus Bletillae (Rhizoma Bletillae) rudiment and growth obtains the Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling for being available for transplanting；The Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate of the present invention sows to only 8 ~ 10 months time for transplanting planting site normal growth more than 80% from seed.
Description
Technical field
The present invention relates to medicinal plants seedling-raising technique field, concretely relates to a kind of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery
Method.
Background technology
Pseudobulbus Bletillae (Rhizoma Bletillae) is orchid family Pseudobulbus Bletillae (Rhizoma Bletillae) platymiscium, is used as medicine with dried root, is mainly used in hemoptysis, is spitted blood, traumatic hemorrhage, skin infection
The diseases such as toxic swelling, chapped skin, ulcerative hemorrhage.Pseudobulbus Bletillae (Rhizoma Bletillae) is a kind of rare Chinese medicine, and the market demand is very big, but due to Pseudobulbus Bletillae (Rhizoma Bletillae) from
Under the conditions of so, breeding is difficult, and wild resource is excavated again in a large number, causes that present Pseudobulbus Bletillae (Rhizoma Bletillae) resource is in imminent danger, and the market price is surging, in order to
Meet the market demand, carried out the research of a large amount of artificial planting techniques, be primarily now carried out by the way of tissue-culturing rapid propagation it is white
And artificial growth, produce Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling, tissue cultured seedling domestication then is transplanted to land for growing field crops, and this method will to facilities and equipment condition
Ask higher, technical difficulty is big, and production stage is loaded down with trivial details, cause the Pseudobulbus Bletillae (Rhizoma Bletillae) seedling for producing relatively costly, limit Pseudobulbus Bletillae (Rhizoma Bletillae) and push away on a large scale
It is wide to develop.
And such as Chinese patent CN201510356671.6, a kind of disclosed Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing breeding method, it includes following
Step：1. the process of fruit and seed：Take Pseudobulbus Bletillae (Rhizoma Bletillae) fresh fruit pod, with flowing water clean, then with 75% ethanol surface sterilization, put water suction
On paper, at the shady and cool ventilation spreading for cooling to dry tack free, by fruit and plant ash volume with 2：1, mix thoroughly, cryopreservation；Before sowing
Peel is removed, seed is taken out, by seed and peat soil or seedling medium volume 1：5, by seed mixing；2. seedling bed prepares：Build
Vertical booth, upper lid Anti-fogging Film, and sunshade net is added a cover, not worked sandy soil are taken, by 8：2 add the fertilizer for fully becoming thoroughly decomposed, mixing
Uniformly, as booth base soil；Booth soil takes out ditch, leveling by the ground width 1m per block, spreads and states booth base soil thickness 10cm, leveling, water spray
To base soil saturation；The peat soil or seedling medium of 1cm are put above, are sprayed water to saturation；3. sow：By the above-mentioned seed handled well by
The amount seed broadcasting of 1 fruit of per square metre of sowing is on seedbed；0.2% Cymbidium ensifolium (L.) Sw. special-purpose foliage fertilizer solution is sprayed to saturation；4. manage
Reason：Keep temperature of shed in 10-30 °C, humidity more than 70%；Per 5 days, 0.2% Cymbidium ensifolium (L.) Sw. special-purpose foliage fertilizer solution was once sprayed in spray
To saturation；Between often water and make booth humidity more than 70%, culture is sprouted and is completed for 1 month.Said method is used seed first
Mixing with seedling medium, booth base soil being mixed with using sandy soil and the fertilizer for becoming thoroughly decomposed afterwards, this processing mode not only can
So that operation becomes complicated, production cost increases, while cannot also ensure that Pseudobulbus Bletillae (Rhizoma Bletillae) sowing is uniform so that presence office in unit area
Portion's seed amount is big, easily brings local nutrition supply not enough, and Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate is low, and less than 70%, long potential difference needs one after transplanting
The domestication of section time, Pseudobulbus Bletillae (Rhizoma Bletillae) are seeded into transplant and successfully last more than 10 months.
Therefore Pseudobulbus Bletillae (Rhizoma Bletillae) sapling multiplication technical costss in the urgent need to address are high, troublesome poeration, and the low problem of Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate is sought
Ask a kind of simple to operate, low production cost, Pseudobulbus Bletillae (Rhizoma Bletillae) seedling breeding method easy to utilize.
The content of the invention
The invention is intended to a kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery is provided, to solve prior art breeding and seedling,
Production technology level is had high demands, cost is also high, Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate is low, after transplanting, need the problem of a period of time domestication.
A kind of method of the Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery in this programme, comprises the following steps：
Step one, get the raw materials ready：The preparation of a, substrate：By parts by weight be 10 ~ 20 parts of pinaster corium farinosum, 20 ~ 40 parts of fertile soil, 30 ~
70 parts of turfy soil is sufficiently mixed makes seedling medium；B, seed sprout the preparation of nutritional solution：Total nutrient content is configured more than 60%
High potassium special fertilizer, wherein nitrogen：Potassium element=1：2, it is added with water solublity phosphoric anhydride, water soluble magnesium and various Chelating states unit
The special fertilizer of element, the ratio of nitrogen are 15 ~ 20%, and the ratio of water solublity phosphoric anhydride is 10 ~ 12%, water soluble magnesium and various chelatings
The ratio summation of state element is between 0.1 ~ 0.3%；Special fertilizer is configured to into 1000 times of liquid, and adds the naphthalene second of 0.4 ~ 0.6mg/L
Acid；
Step 2, selection of land bedding：Select water source convenient, sunny, well-drained land for growing field crops sandy loam makes wide 1 meter flat
Furrow；
Step 3, furrow upper berth substrate：The substrate for preparing is laid on furrow, thickness is formed in the seedbed of 8 ~ 10cm；
Step 4, sowing：By Pseudobulbus Bletillae (Rhizoma Bletillae) seed uniform broadcasting on seedbed, seed density is per square metre broadcasts sowing the kind of 2 Fruit pods
Son amount；
Step 5, spray nutritious liquor：The seed for preparing sprouting nutritional solution is watered by spray pattern, irrigating makes substrate
Abundant saturation；
Step 6, do shed：Irrigate after seed sprouts nutritional solution and Small plastic shed is built on seedbed, cover plastic sheeting；
Step 7, temperature control moisturizing：The temperature and humidity in monitoring seedbed, makes temperature control at 20 ~ 30 DEG C, humid control 60 ~
90%, one time of nutrition liquid was sprayed every 3 days, seed greening in 8 ~ 15 days stops spray nutritious liquor after sprouting；
Step 8, spray foliage fertilizer：After seed is sprouted and sprouted 5 days start a foliage fertilizer was sprayed every 15 days；
Step 9, remove plastic sheeting：Plastic sheeting is removed after seed grows 2 ~ 3 leaves；
Step 10, transplanting：Growth treated that Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling length reaches more than 8cm after 8 ~ 10 months, and tuber diameter reaches 1.5 ~ 3cm,
Fall before seedling on seedbed in November Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling then to be transplanted to planting site and grow naturally.
The operation principle and beneficial effect of the present invention：1st, be pre-configured with seedling medium, seedling medium be using pinaster corium farinosum,
Turfy soil and fertile soil are formulated according to a certain percentage, take into full account that Pseudobulbus Bletillae (Rhizoma Bletillae) seed sprouts required nutritional labeling；Bark
Powder is lightweight, the content of organic matter is high, water-retaining property is preferable, breathability is strong and with higher cation exchange capacity (CEC), while may be used also
To provide the trace element needed for plant growing, rich in abundant humus, mineral and organic matter in turfy soil and fertile soil,
Nutrition for needed for the sprouting and the later growth of seedling of Pseudobulbus Bletillae (Rhizoma Bletillae) provide each growth period, and guarantee the growth vigor of abundance, with
The single seedling medium of prior art is compared, and is had also more balanced, is more suitable for the growth of Pseudobulbus Bletillae (Rhizoma Bletillae).2nd, directly seedling medium is placed
Use as seedbed on furrow, Pseudobulbus Bletillae (Rhizoma Bletillae) seed is being sowed on seedbed, seedling medium sufficiently can not only be connect with Pseudobulbus Bletillae (Rhizoma Bletillae) seed
Touch, make state of the Pseudobulbus Bletillae (Rhizoma Bletillae) seed in eutrophication, being mixed to be sowed Pseudobulbus Bletillae (Rhizoma Bletillae) seed with substrate again with prior art is being had
For on the seedbed of soil, more can determine that nutrient substance is balanced everywhere, be not in the not enough problem of local nutrition supply.3rd, section
Configuration seed sprouts nutritional solution, is more than 60% high potash fertilizer containing total nutrient content, while also containing nitrogen in planting germination fluid
The element of element, water solublity phosphoric anhydride, water soluble magnesium and various Chelating states, the wherein element of Chelating state are mainly Pseudobulbus Bletillae (Rhizoma Bletillae) growth institute
Necessary trace element, Chelating state element be it is water miscible, it is higher to crop effectiveness, trace element can be reduced after being manured into soil
By soil fixing, effect is better than inorganic salts, and consumption is also less, while the trace element of Chelating state has high Jing after chelating
Biological activity, its effect is tens times of general organic trace element fertilizer, the hundred times of inorganic salts；The metal ion of trace element is by chela
After conjunction, less organic molecule is formed, absorbed with organic molecule state by crop, conversion is directly participated in in crop body；Dissolving compared with
High, good stability, not easily runs off, slow release, supplements rapidly nutrient, improves the utilization rate of germination fluid.4th, directly in land
Nursery culture is carried out on earth, is made Pseudobulbus Bletillae (Rhizoma Bletillae) that the environment in progressively reform of nature and land for growing field crops is begun in the seed growth stage, is later
Transplanting lays the first stone, and reduces prior art and is transplanted to the time that field adapts to environment from booth or greenhouse, while also avoiding the occurrence of
Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling problem of death because of change like a fish out of water.5th, after Pseudobulbus Bletillae (Rhizoma Bletillae) seed is sowed, seed is sprouted nutrient solution spray and is watered, and pours
Thoroughly, in the germination period of seed, keep 3 days once pour nutritional solution, it is ensured that the humidity and temperature of seedling medium, make Pseudobulbus Bletillae (Rhizoma Bletillae) in most
It is suitable in the environment sprouted, stops pouring nutritional solution after seed is sprouted；Seed germination sprayed foliage fertilizer every 15 days after 5 days, and
When remove plastic sheeting, be transplanted to planting site at November then.The present invention compared with prior art, by scientific and reasonable
Configuration seedling medium and seed sprout nutritional solution, it is live in big Tanaka, seed is directly sowed the seedling medium on land for growing field crops
On, then irrigated with seed germination fluid, it is ensured that the nutrition required for Pseudobulbus Bletillae (Rhizoma Bletillae) rudiment and growth, it is daily finally by strict control
Management, including humidity and temperature and water and fertilizer management, obtains the Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling for being available for transplanting；The present invention Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate 80% with
On, only 8 ~ 10 months time for transplanting planting site normal growth is sowed to from seed.
Further, in the step one, seedling medium by the Cortex Pini, 20 parts of fertile soil that parts by weight are 10 parts and
30 parts of turfy soil configuration is formed.The seedling medium of this proportional arrangement is more preferable to the sprouting of Pseudobulbus Bletillae (Rhizoma Bletillae) and growth result.
Further, in the step one, the seedling medium for preparing sterilizes at 80 ~ 90 DEG C 8 ~ 12min.Sterilization in advance disappears
Poison is processed, it is to avoid growth of the grass seeds or other microorganisms in seedling medium to Pseudobulbus Bletillae (Rhizoma Bletillae) is impacted.
The further restriction of nutritional solution is sprouted as seed, in the step one, the nitrogen in special fertilizer is water solublity
Nitrogen, ratio are 15%, and potassium element is water-soluble potassium, and ratio is 30%, and the ratio of water solublity phosphoric anhydride is 10%, the ratio of water soluble magnesium
For 0.05%, the species of Chelating state element is Chelating state boron, Chelating state copper, chelated ferrous iron, Chelating state manganese, Chelating state molybdenum, Chelating state
One or more in zinc, the ratio of Chelating state element is 0.05 ~ 0.25%.Configure as required, when under this ratio, Pseudobulbus Bletillae (Rhizoma Bletillae) is sprouted
Between it is short, germination rate is up to 85%.
Further, when the step 4 is sowed, Pseudobulbus Bletillae (Rhizoma Bletillae) seed is taken out, is positioned in bottle, the yarn in bottleneck upper cover
Net, bottleneck is overturned, shake sowing.The particle diameter of Pseudobulbus Bletillae (Rhizoma Bletillae) seed is less, in order to ensure uniformly to sow, by aforesaid operations come really
It is fixed.
Description of the drawings
Fig. 1 is a kind of comparison diagram of the method with tissue culture of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery of the invention.
Specific embodiment
Below by specific embodiment, the present invention is further detailed explanation：
Embodiment 1：A kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery, comprises the following steps：
Step one, get the raw materials ready：The preparation of a, substrate：It is 10 parts of pinaster corium farinosum, 20 parts of fertile soil, 30 parts of turf by parts by weight
Soil is sufficiently mixed makes seedling medium, and the seedling medium for preparing sterilizes at 80 DEG C 12min；B, seed sprout the system of nutritional solution
It is standby：High potassium special fertilizer of total nutrient content more than 60% is configured, nitrogen is water-soluble nitrogen, and ratio is 15%, potassium element is water-soluble
Property potassium, ratio is 30%, and the ratio of water solublity phosphoric anhydride is 10%, and the ratio of water soluble magnesium is 0.05%, and the ratio of Chelating state boron is
0.007%th, the ratio of Chelating state copper be ratio that the ratio of 0.004%, chelated ferrous iron is 0.05%, Chelating state manganese be 0.025%, chela
It is 0.0025% that the ratio for closing state molybdenum is the ratio of 0.001%, Chelating state zinc；Special fertilizer is configured to into 1000 times of liquid, and is added
The naphthalene acetic acid of 0.5mg/L；
Step 2, selection of land bedding：Select water source convenient, sunny, well-drained land for growing field crops sandy loam makes wide 1 meter flat
Furrow；
Step 3, furrow upper berth substrate：The substrate for preparing is laid on furrow, thickness is formed in the seedbed of 8cm；
Step 4, sowing：During sowing, Pseudobulbus Bletillae (Rhizoma Bletillae) seed is taken out, is positioned in bottle, bottleneck is run by the gauze in bottleneck upper cover
, shake sowing, by Pseudobulbus Bletillae (Rhizoma Bletillae) seed uniform broadcasting on seedbed, seed density is per square metre broadcasts sowing the grain weight of 2 Fruit pods；
Step 5, spray nutritious liquor：The seed for preparing sprouting nutritional solution is watered by spray pattern, irrigating makes substrate
Abundant saturation；
Step 6, do shed：Irrigate after seed sprouts nutritional solution and Small plastic shed is built on seedbed, cover plastic sheeting；
Step 7, temperature control moisturizing：The temperature and humidity in monitoring seedbed, makes temperature control at 25 DEG C, humid control 90%, every 3
Its spray one time of nutrition liquid, seed greening in 8 ~ 15 days stop spray nutritious liquor after sprouting；
Step 8, spray foliage fertilizer：After seed is sprouted and sprouted 5 days start a foliage fertilizer was sprayed every 15 days；
Step 9, remove plastic sheeting：Plastic sheeting is removed after seed grows 2 ~ 3 leaves；
Step 10, transplanting：Growth treated that Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling length reaches more than 8cm after 8 ~ 10 months, and tuber diameter reaches 1.5 ~ 3cm,
Fall before seedling on seedbed in November Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling then to be transplanted to planting site and grow naturally.
Using the method for embodiment 1, Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate sows transplanting planting site normal growth from seed more than 85%
Time be only 6 months, during transplanting, Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling length reaches more than 8cm, and tuber diameter is up to 3.5cm, survival rate after transplanting
95%。
Embodiment 2 with the only difference of embodiment 1 is：
Step one, get the raw materials ready：The preparation of a, substrate：It is 20 parts of pinaster corium farinosum, 30 parts of fertile soil, 50 parts of turf by parts by weight
Soil is sufficiently mixed makes seedling medium, and the seedling medium for preparing sterilizes at 90 DEG C 8min；B, seed sprout the system of nutritional solution
It is standby：High potassium special fertilizer of total nutrient content more than 60% is configured, nitrogen is water-soluble nitrogen, and ratio is 20%, potassium element is water-soluble
Property potassium, ratio is 40%, and the ratio of water solublity phosphoric anhydride is 12%, and the ratio of water soluble magnesium is 0.06%, and the ratio of Chelating state boron is
0.007%th, the ratio of Chelating state copper be ratio that the ratio of 0.004%, chelated ferrous iron is 0.07%, Chelating state manganese be 0.03%, chela
It is 0.0025% that the ratio for closing state molybdenum is the ratio of 0.001%, Chelating state zinc；Special fertilizer is configured to into 1000 times of liquid, and is added
The naphthalene acetic acid of 0.6mg/L；
Step 7, temperature control moisturizing：The temperature and humidity in monitoring seedbed, makes temperature control at 20 DEG C, humid control 60%, every 3
Its spray one time of nutrition liquid, seed greening in 8 ~ 15 days stop spray nutritious liquor after sprouting.
Using the method for embodiment 2, Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate sows transplanting planting site normal growth from seed more than 80%
Time be only 8 months, during transplanting, Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling length reaches more than 7cm, and tuber diameter is up to 3cm, survival rate 95% after transplanting.
Embodiment 3 with the only difference of embodiment 1 is：
Step one, get the raw materials ready：The preparation of a, substrate：It is 20 parts of pinaster corium farinosum, 40 parts of fertile soil, 70 parts of turf by parts by weight
Soil is sufficiently mixed makes seedling medium, the seedling medium for preparing high temperature sterilization 10min at 84 DEG C；B, seed sprout nutritional solution
Preparation：High potassium special fertilizer of total nutrient content more than 60% is configured, nitrogen is water-soluble nitrogen, and ratio is 17%, and potassium element is
Water-soluble potassium, ratio are 34%, and the ratio of water solublity phosphoric anhydride is 11%, and the ratio of water soluble magnesium is 0.06%, the ratio of Chelating state boron
For 0.007%, Chelating state copper ratio be 0.004%, chelated ferrous iron ratio be 0.07%, Chelating state manganese ratio be 0.03%,
It is 0.0025% that the ratio of Chelating state molybdenum is the ratio of 0.001%, Chelating state zinc；Special fertilizer is configured to into 1000 times of liquid, and is added
The naphthalene acetic acid of 0.4mg/L；
Step 7, temperature control moisturizing：The temperature and humidity in monitoring seedbed, makes temperature control at 25 DEG C, humid control 80%, every 3
Its spray one time of nutrition liquid, seed greening in 8 ~ 15 days stop spray nutritious liquor after sprouting.
Using the method for embodiment 3, Pseudobulbus Bletillae (Rhizoma Bletillae) germination rate more than 83%, sow from seed more than 98% by seed utilization rate
To the only 7 months time for transplanting planting site normal growth, during transplanting, Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling length reaches more than 7cm, and tuber diameter is up to
2.8cm, survival rate 95% after transplanting.
Direct seeding technique, existing tissue culture technology and background technology CN201510356671.6（Hereinafter referred to as correlation technique）'s
Contrast.
On time
1.1 cultivate tissue cultured seedling by tissue culture needs 6 ~ 8 months, and booth domestication tissue cultured seedling needs 6 months, then by tissue culture skill
Art then needs 12 ~ 14 month to Pseudobulbus Bletillae (Rhizoma Bletillae) transplantation of seedlings from seed.
1.2 then need 8 ~ 10 months by correlation technique from seed to Pseudobulbus Bletillae (Rhizoma Bletillae) transplantation of seedlings, need domestication 2 ~ 4 months.
1.3 Pseudobulbus Bletillae (Rhizoma Bletillae) direct seeding techniques are then only needed to from seed to Pseudobulbus Bletillae (Rhizoma Bletillae) transplantation of seedlings 8 ~ 10 months, without the need for domestication, can be saved at least
2 months.
Germination rate
The germination rate of 2.1 Pseudobulbus Bletillae (Rhizoma Bletillae) tissue culture technologies is 90% or so, but pollution rate is also very high during tissue culture, under COMPREHENSIVE CALCULATING
Carry out the seed utilization rate of Pseudobulbus Bletillae (Rhizoma Bletillae) tissue culture technology less than 50%.
2.2 by the germination rate of correlation technique Pseudobulbus Bletillae (Rhizoma Bletillae) 70% or so.
The germination rate of 2.3 Pseudobulbus Bletillae (Rhizoma Bletillae) direct seeding techniques is more than 83%, although for the germination rate of seed is not dominant compared with tissue culture
But there is no the situation of seed contamination in gesture direct seeding technique, seed utilization rate is more than 98%.
Adaptation after transplanting to environment
3.1, Pseudobulbus Bletillae (Rhizoma Bletillae) tissue cultured seedling needs the domestication through laboratory cultures and booth to need to place environment temperature control, controls wet, control light
According to.So tissue cultured seedling needs the regular hour gradually to adapt to extraneous natural environment after being transplanted to land for growing field crops, need after transplanting
The temporal adaptation external world natural environment for taking 2 ~ 3 months.
3.2 correlation technique Pseudobulbus Bletillae (Rhizoma Bletillae) be in the environment of warmhouse booth sow, growth, need after transplanting 2 ~ 3 months when
Between adapt to extraneous natural environment.
The live Seedling of 3.3 Pseudobulbus Bletillae (Rhizoma Bletillae) due to being directly to sow under natural environment, growth, reform of nature that can be quickly after transplanting
Environment, without the need for domestication.
As shown in figure 1, comparing with Pseudobulbus Bletillae (Rhizoma Bletillae) sowing in the field for the domestication of tissue culture booth, the Pseudobulbus Bletillae (Rhizoma Bletillae) of the present invention is live better than group
Training technology.
Above-described is only embodiments of the invention, and in scheme, the known general knowledge here such as concrete structure and characteristic is not made
It is excessive to describe.It should be pointed out that for a person skilled in the art, on the premise of without departing from present configuration, can be with
Some deformations and improvement are made, these should also be considered as protection scope of the present invention, these are implemented all without the impact present invention
Effect and practical applicability.This application claims protection domain should be defined by the content of its claim, in description
Specific embodiment etc. records the content that can be used for explaining claim.
Claims (5)
1. a kind of method of Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery, it is characterised in that comprise the following steps：
Step one, get the raw materials ready：The preparation of a, substrate：By parts by weight be 10 ~ 20 parts of pinaster corium farinosum, 20 ~ 40 parts of fertile soil, 30 ~
70 parts of turfy soil is sufficiently mixed makes seedling medium；B, seed sprout the preparation of nutritional solution：Total nutrient content is configured more than 60%
High potassium special fertilizer, wherein nitrogen：Potassium element=1：2, it is added with water solublity phosphoric anhydride, water soluble magnesium and various Chelating states unit
The special fertilizer of element, the ratio of nitrogen are 15 ~ 20%, and the ratio of water solublity phosphoric anhydride is 10 ~ 12%, water soluble magnesium and various chelatings
The ratio summation of state element is between 0.1 ~ 0.3%；Special fertilizer is configured to into 1000 times of liquid, and adds the naphthalene second of 0.4 ~ 0.6mg/L
Acid；
Step 2, selection of land bedding：Select water source convenient, sunny, well-drained land for growing field crops sandy loam makes wide 1 meter flat
Furrow；
Step 3, furrow upper berth substrate：The substrate for preparing is laid on furrow, thickness is formed in the seedbed of 8 ~ 10cm；
Step 4, sowing：By Pseudobulbus Bletillae (Rhizoma Bletillae) seed uniform broadcasting on seedbed, seed density is per square metre broadcasts sowing the kind of 2 Fruit pods
Son amount；
Step 5, spray nutritious liquor：The seed for preparing sprouting nutritional solution is watered by spray pattern, irrigating makes substrate
Abundant saturation；
Step 6, do shed：Irrigate after seed sprouts nutritional solution and Small plastic shed is built on seedbed, cover plastic sheeting；
Step 7, temperature control moisturizing：The temperature and humidity in monitoring seedbed, makes temperature control at 20 ~ 30 DEG C, humid control 60 ~
90%, one time of nutrition liquid was sprayed every 3 days, seed greening in 8 ~ 15 days stops spray nutritious liquor after sprouting；
Step 8, spray foliage fertilizer：After seed is sprouted and sprouted 5 days start a foliage fertilizer was sprayed every 15 days；
Step 9, remove plastic sheeting：Plastic sheeting is removed after seed grows 2 ~ 3 leaves；
Step 10, transplanting：Growth treated that Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling length reaches more than 8cm after 8 ~ 10 months, and tuber diameter reaches 1.5 ~ 3cm,
Fall before seedling on seedbed in November Pseudobulbus Bletillae (Rhizoma Bletillae) Seedling then to be transplanted to planting site and grow naturally.
2. the method for Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery according to claim 1, it is characterised in that：In the step one,
Seedling medium is formed by the turfy soil configuration of the Cortex Pini, 20 parts of fertile soil that parts by weight are 10 parts and 30 parts.
3. the method for Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery according to claim 2, it is characterised in that：In the step one,
The seedling medium for preparing sterilizes at 80 ~ 90 DEG C 8 ~ 12min.
4. the method for Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery according to claim 3, it is characterised in that：In the step one,
Nitrogen in special fertilizer is water-soluble nitrogen, and ratio is 15%, and potassium element is water-soluble potassium, and ratio is 30%, water solublity phosphoric anhydride
Ratio is 10%, and the ratio of water soluble magnesium is 0.05%, the species of Chelating state element be Chelating state boron, Chelating state copper, chelated ferrous iron,
One or more in Chelating state manganese, Chelating state molybdenum, Chelating state zinc, the ratio of Chelating state element is 0.05 ~ 0.25%.
5. the method for Pseudobulbus Bletillae (Rhizoma Bletillae) seed sowing in the field nursery according to claim 4, it is characterised in that：The step 4 sowing
When, Pseudobulbus Bletillae (Rhizoma Bletillae) seed being taken out, is positioned in bottle, bottleneck is overturned by the gauze in bottleneck upper cover, shake sowing.
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| CN108029490A (en) * | 2018-01-19 | 2018-05-15 | 遵义琦琅生物技术咨询有限公司 | A kind of bletilla striata seeds direct sowing and seedling matrix and its method for culturing seedlings |
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