CN102301955A - Method for breeding rehmannia toxin-free seedling - Google Patents
Method for breeding rehmannia toxin-free seedling Download PDFInfo
- Publication number
- CN102301955A CN102301955A CN201110209583A CN201110209583A CN102301955A CN 102301955 A CN102301955 A CN 102301955A CN 201110209583 A CN201110209583 A CN 201110209583A CN 201110209583 A CN201110209583 A CN 201110209583A CN 102301955 A CN102301955 A CN 102301955A
- Authority
- CN
- China
- Prior art keywords
- glutinous rehmannia
- virus
- test tube
- toxin
- tube plantlet
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for breeding a rehmannia toxin-free seedling. The method disclosed by the invention comprises the following steps: production of an original rehmannia seedling: cutting a rehmannia toxin-free test-tube plantlet into stem segments provided with axillary buds with the length of about 1cm on a clean bench, inoculating the stem segments onto a culture medium with the concentration of MS+NAA of being 0.01-0.05mg/L and the concentration of 6-BA of being 0.05-0.5mg/L, and culturing under the culture conditions of light intensity of 2000lux, pH of 6.0 and temperature at 25-30 DEG C to obtain a toxin-free test tube plantle, wherein, reproduction coefficient can reach about 5 after 30-40 days; cutting the rehmannia toxin-free test tube plantlet into the stem segments provided with the axillary buds with the length of about 1cm on the clean bench, inoculating the stem segments onto a culture medium with the concentration of 1/2MS+PPP<333> of being 0.02-0.2mg/L, and culturing in a culturing room to obtain a rooted toxin-free test tube plantlet, wherein, roots are formed within 7 days and a large number of roots are formed within 15-20 days; and transplanting the test tube plantlet into a greenhouse to carry out acclimation so as to finally obtain the toxin-free seedling, wherein, transplanting survival rate can reach up to more than 95%. The method disclosed by the invention has the advantages that cost for breeding the toxin-free seedling is low and yield is high.
Description
Technical field:
The present invention relates to a kind of seedling breeding method, particularly a kind of mating system of glutinous rehmannia virus-elimination seedlings.
Background technology:
The bosom glutinous rehmannia is one of China's famous " four-Huai medicine " and traditional Chinese medicines material, in Chinese herbal medicine, occupies extremely important status, but because long-term nutrition breeding, and virus infections serious (the field infection rate reaches 100%) causes that output seriously descends, quality deterioration.Therefore how to remove the virus that infects the bosom glutinous rehmannia, improve its output, improve its quality, searching out the raising technology system that can and make the rapid popularizing planting of its virus-elimination seedlings has become the important topic of being badly in need of solution in the production.
Summary of the invention:
The mating system that the purpose of this invention is to provide the low a kind of glutinous rehmannia virus-elimination seedlings of a kind of good quality and high output and cost.The technical scheme that realizes the object of the invention is; A kind of mating system of glutinous rehmannia virus-elimination seedlings; It is characterized in that following steps are arranged: the production of (1) one-level glutinous rehmannia virus-elimination seedlings, on the superclean bench glutinous rehmannia detoxification test tube plantlet being cut on the medium that stem segment with axillary bud is inoculated in MS+NAA 0.01～0.05mg/L+6-BA0.05～0.5mg/L, under light intensity 2000lux, pH6.0 and temperature 25-30 ℃ condition of culture, cultivate; 30～40d breeds the glutinous rehmannia detoxification test tube plantlet; On the superclean bench above-mentioned glutinous rehmannia detoxification test tube plantlet is being cut on the medium that stem segment with axillary bud is inoculated in 1/2,MS+,PPP,333 0.02～0.2mg/L; Under culturing room's condition, cultivate; 7d begins to have the formation of root; 15～20d can form a large amount of roots, brings out the glutinous rehmannia detoxification test tube plantlet of root, above-mentioned glutinous rehmannia detoxification test tube plantlet immigration greenhouse of taking root is tamed promptly obtain the one-level virus-elimination seedlings; (2) production of secondary glutinous rehmannia virus-elimination seedlings moves into the underground of insect protected solarium with the one-level glutinous rehmannia virus-elimination seedlings of step (1), and Routine Management after 2 months, with underground root results, is cut into the fritter that has eye, obtains secondary glutinous rehmannia virus-elimination seedlings; The production of (3) three grades of glutinous rehmannia virus-elimination seedlingses, the secondary bosom glutinous rehmannia virus-elimination seedlings immigration of step (2) is underground, in November or March next year underground root being gathered in the crops then, be divided into the fritter that has eye, promptly obtain three grades of glutinous rehmannia virus-elimination seedlingses, be used for field planting.The present invention and prior art relatively have virus-elimination seedlings and breed the low remarkable advantage with output height and quality better of cost.
Embodiment:
In conjunction with following examples the present invention is elaborated:
(1), the production of one-level bosom glutinous rehmannia virus-elimination seedlings; To be cut into length be that stem segment with axillary bud about 1cm is inoculated on the medium of MS+NAA 0.01～0.05mg/L+6-BA0.05～0.5mg/L cherishing the glutinous rehmannia detoxification test tube plantlet on the superclean bench; Under light intensity 2000lux, pH6.0 and temperature 25-30 ℃ condition of culture, cultivate; Behind 30～40d, reproduction coefficient can reach about 5, breeds detoxification test tube plantlet; On the superclean bench above-mentioned bosom glutinous rehmannia detoxification test tube plantlet being cut into length is that stem segment with axillary bud about 1cm is inoculated in 1/2MS+PPP
3330.02 on the medium of～0.2mg/L, under culturing room's condition, cultivate, 7d begins to have the formation of root, 15～20d can form a large amount of roots, brings out the detoxification test tube plantlet of root; Above-mentioned detoxification test tube plantlet of taking root is moved into the greenhouse tame and promptly obtain one-level bosom glutinous rehmannia virus-elimination seedlings, transplanting survival rate can reach more than 95%.
(2), the production of secondary bosom glutinous rehmannia virus-elimination seedlings; The one-level bosom glutinous rehmannia virus-elimination seedlings that step (1) is bred is transplanted underground into the insect protected solarium April～May, and water and fertilizer management is given in the centre, 7～August; With underground root results; Be cut into the fritter that has eye, obtain secondary bosom glutinous rehmannia virus-elimination seedlings, each one-level seedling can be produced 10～15 secondary virus-elimination seedlingses.
(3), the production of three grades of bosom glutinous rehmannia virus-elimination seedlingses; Move into the secondary bosom glutinous rehmannia virus-elimination seedlings piece of step (2) underground; In gathering in the crops in November or March next year then, be divided into the fritter that has eye again, promptly obtain three grades of bosom glutinous rehmannia virus-elimination seedlingses; Each secondary bosom glutinous rehmannia virus-elimination seedlings can obtain 10～15 three grades bosom glutinous rehmannia virus-elimination seedlingses, directly is used for field planting.
Claims (1)
1. the mating system of a glutinous rehmannia virus-elimination seedlings; It is characterized in that following steps are arranged: the production of (1) one-level glutinous rehmannia virus-elimination seedlings; On the superclean bench glutinous rehmannia detoxification test tube plantlet is being cut on the medium that stem segment with axillary bud is inoculated in MS+NAA 0.01～0.05mg/L+6-BA0.05～0.5mg/L; Under light intensity 2000lux, pH6.0 and temperature 25-30 ℃ condition of culture, cultivate, 30～40d breeds the glutinous rehmannia detoxification test tube plantlet; On superclean bench, above-mentioned glutinous rehmannia detoxification test tube plantlet is cut into stem segment with axillary bud and is inoculated in 1/2MS+PPP
3330.02 on the medium of～0.2mg/L; Under culturing room's condition, cultivate, 7d begins to have the formation of root, and 15～20d can form a large amount of roots; Bring out the glutinous rehmannia detoxification test tube plantlet of root, above-mentioned glutinous rehmannia detoxification test tube plantlet immigration greenhouse of taking root is tamed promptly obtain the one-level virus-elimination seedlings; (2) production of secondary glutinous rehmannia virus-elimination seedlings moves into the underground of insect protected solarium with the one-level glutinous rehmannia virus-elimination seedlings of step (1), and Routine Management after 2 months, with underground root results, is cut into the fritter that has eye, obtains secondary glutinous rehmannia virus-elimination seedlings; The production of (3) three grades of glutinous rehmannia virus-elimination seedlingses, the secondary bosom glutinous rehmannia virus-elimination seedlings immigration of step (2) is underground, in November or March next year underground root being gathered in the crops then, be divided into the fritter that has eye, promptly obtain three grades of glutinous rehmannia virus-elimination seedlingses, be used for field planting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110209583A CN102301955A (en) | 2011-07-19 | 2011-07-19 | Method for breeding rehmannia toxin-free seedling |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110209583A CN102301955A (en) | 2011-07-19 | 2011-07-19 | Method for breeding rehmannia toxin-free seedling |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102301955A true CN102301955A (en) | 2012-01-04 |
Family
ID=45375960
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110209583A Pending CN102301955A (en) | 2011-07-19 | 2011-07-19 | Method for breeding rehmannia toxin-free seedling |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102301955A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657090A (en) * | 2012-05-17 | 2012-09-12 | 福建农林大学 | Rapid propagation method of rehmannia by tissue culture |
| CN103975722A (en) * | 2014-04-25 | 2014-08-13 | 山西省农业科学院棉花研究所 | Rehmannia virus-free test tube seedling net house direct soil entering transplanting method |
| CN104350913A (en) * | 2014-10-23 | 2015-02-18 | 周琪 | Seedling raising method for rehmannia piasezkii |
| CN104380954A (en) * | 2014-10-23 | 2015-03-04 | 周琪 | Seedling raising method of rehmannia chingii |
| CN104705023A (en) * | 2013-12-12 | 2015-06-17 | 李振达 | Technology for planting traditional Chinese medicine radix rehmanniae recen |
| CN105766250A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Method for planting Rehmannia glutinosa |
| CN108496600A (en) * | 2018-04-24 | 2018-09-07 | 山西省农业科学院作物科学研究所 | A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method |
| CN108812306A (en) * | 2018-04-28 | 2018-11-16 | 河南师范大学 | Chinese medicine glutinous rehmannia tissue cultures expanding propagation method is carried out using interval submergence bioreactor |
| CN113197093A (en) * | 2021-05-21 | 2021-08-03 | 河南中医药大学 | Detoxification method of rehmannia glutinosa |
-
2011
- 2011-07-19 CN CN201110209583A patent/CN102301955A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 于相丽，杨柯金: "怀地黄的研究进展", 《安徽农业科学》 * |
| 李明军等: "怀地黄脱毒种苗规模化生产技术研究", 《河南省植物生理学会三十周年庆典暨学术研讨会》 * |
| 李明军等: "怀地黄试管苗脱毒技术研究", 《河南师范大学学报（自然科学版）》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102657090A (en) * | 2012-05-17 | 2012-09-12 | 福建农林大学 | Rapid propagation method of rehmannia by tissue culture |
| CN104705023A (en) * | 2013-12-12 | 2015-06-17 | 李振达 | Technology for planting traditional Chinese medicine radix rehmanniae recen |
| CN103975722A (en) * | 2014-04-25 | 2014-08-13 | 山西省农业科学院棉花研究所 | Rehmannia virus-free test tube seedling net house direct soil entering transplanting method |
| CN104350913A (en) * | 2014-10-23 | 2015-02-18 | 周琪 | Seedling raising method for rehmannia piasezkii |
| CN104380954A (en) * | 2014-10-23 | 2015-03-04 | 周琪 | Seedling raising method of rehmannia chingii |
| CN104380954B (en) * | 2014-10-23 | 2016-08-24 | 周口师范学院 | A kind of method for culturing seedlings of root of Ching Rehmannia |
| CN104350913B (en) * | 2014-10-23 | 2016-12-07 | 南通市乐悦实业有限公司 | A kind of method for culturing seedlings of decomposite leaf Radix Rehmanniae |
| CN105766250A (en) * | 2014-12-22 | 2016-07-20 | 重庆市万盛经开区黑山镇供销合作社有限公司 | Method for planting Rehmannia glutinosa |
| CN108496600A (en) * | 2018-04-24 | 2018-09-07 | 山西省农业科学院作物科学研究所 | A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method |
| CN108812306A (en) * | 2018-04-28 | 2018-11-16 | 河南师范大学 | Chinese medicine glutinous rehmannia tissue cultures expanding propagation method is carried out using interval submergence bioreactor |
| CN113197093A (en) * | 2021-05-21 | 2021-08-03 | 河南中医药大学 | Detoxification method of rehmannia glutinosa |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102301955A (en) | Method for breeding rehmannia toxin-free seedling | |
| CN104686332A (en) | Method for establishing tissue culture regeneration system of camellia sinensis L. | |
| CN105028209B (en) | A kind of method for improving Vaccinium ashei tissue culture seedling rooting | |
| CN103385168B (en) | Method for regeneration plant of tung oil tree leaf | |
| CN105075863B (en) | A kind of Paeonia papaveracea rapid propagation method | |
| CN104938331A (en) | Tissue culture propagation method of dendrobium officinale | |
| CN103380729B (en) | A kind of method of biplate lettuce tongue tissue cultures and Fast-propagation | |
| CN104304029A (en) | Peony tissue culture and rapid propagation method | |
| CN104969731A (en) | Method for raising seedlings of American red maple red sunset by cutting | |
| CN103430845A (en) | Strawberry tissue culturing method | |
| CN104472365B (en) | Rich and honour banyan tissue-culturing rapid propagation method for culturing seedlings | |
| CN103461129B (en) | Tissue culture and intermediate propagation method of chirita anachoreta | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN102939902A (en) | Rapid propagation method of amorphophallus bulbifer through tissue culture | |
| CN102273405B (en) | Cultivating method of Hosta plantaginea Gold Standard | |
| CN103371103A (en) | Rapid propagation method for tissue culture of Rhododendron delavayi Franch | |
| CN102415339A (en) | Rapid propagation method of photinia fraseri | |
| CN103583372B (en) | Eucommia ulmoides Oliv. in-vitro rapid-propagation method | |
| CN102860256A (en) | Oil palm tissue culture method | |
| CN102613080A (en) | Method for rapidly propagating wild ginseng | |
| CN109122315B (en) | Method for cultivating seedlings by using alum root and petioles | |
| CN104335898B (en) | A kind of method of Skimmia japonica Rubella Vitro Quick Reproduction | |
| CN103583371B (en) | Tissue culture method for improved variety of Populus davidiana Dode--P. alba*P. davidian CL. 1333 | |
| CN104686336A (en) | Tissue culture rapid propagation method of ailanthus altissima | |
| CN105941143B (en) | A kind of detoxification of lily bulbs method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120104 |