CN108496600A - A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method - Google Patents
A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method Download PDFInfo
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- CN108496600A CN108496600A CN201810375122.7A CN201810375122A CN108496600A CN 108496600 A CN108496600 A CN 108496600A CN 201810375122 A CN201810375122 A CN 201810375122A CN 108496600 A CN108496600 A CN 108496600A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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Abstract
The present invention is specially a kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method, and solving the problems, such as the breeding of existing glutinous rehmannia test tube seedling, there are inefficient operations, of high cost and easy to be dead.A, detoxic radix rehmanniae test tube seedling is cut into stem with bud to cultivate, cultivation temperature is 25 DEG C 28 DEG C, and intensity of illumination is 2500Lx 3000Lx, and light application time is 8 hours/day；B, it is cut into the stem with bud of one bud of a leaf under open environment, and is soaked in tap water at once；C, cuttage is in seedlings nursing plate, per two, cave stem with bud, cuttage position in it is diagonal it is linear be distributed, and stem with bud is before growing sprouting, and the humidity in seedlings nursing plate is 90% 95%, and temperature is 15 DEG C 30 DEG C, and intensity of illumination is 1500Lx~2000Lx under film；D, crop field is transplanted after cuttage seeding grows sprouting and root completes quickly breeding.Breeding amount greatly improved in the present invention, reduces the cost of detoxic radix rehmanniae plantlet bud propagation.
Description
Technical field
The present invention relates to the propagation method of test tube seedling in agricultural planting technique, specially a kind of micro- skewer of detoxic radix rehmanniae test tube seedling
Insert rapid propagation method.
Background technology
The Chinese medicine dosage of glutinous rehmannia is larger, about 600,000 mu of China's annual planting area, according to investigations the regional glutinous rehmannia growing surface in Shanxi south
Product accounts for 50% or more of China's glutinous rehmannia cultivated area at present.However glutinous rehmannia uses root block to carry out vegetative propagation for a long time in production,
By the harm of the pathogens such as dehuang degradation virus (DDV), glutinous rehmannia X viruses and CMV, Shi Di yellow race sexual involution seriously affects production
Amount and quality.For a long time, there are research institute or university by stem apex detoxification one by one, detoxification is produced with test tube seedling fast breeding technique
Seedling.
The yield and quality of glutinous rehmannia is obviously greatly improved in this technology, it can be difficult to the bottleneck problem abolished is：
1, the requirement of inefficient operation, sterile working itself affects service speed；2, cost of material is high, test tube seedling to culture medium at
Divide more demanding and needs a large amount of culture bottle；3, house consumption is big, and Tube propagation easily goes out invalid vitreous shoot, and in addition bacterium pollution causes to try
Pipe seedling is dead；4, complicated transitional processes are needed, in addition excessive initial stage maximum temperature slightly then causes accidentally no more than 28 DEG C
Mortality.
Therefore, although detoxic radix rehmanniae there are many merits, make great efforts to promote for many years through country and relevant departments of local governments at various levels,
The effect is unsatisfactory for it, and the glutinous rehmannia seedling largely used in glutinous rehmannia production at present is still that peasant plants certainly from numerous, virus-elimination seedlings
Using very little.
Invention content
The present invention in order to solve the breeding of existing glutinous rehmannia test tube seedling there are inefficient operation, of high cost and easy to be dead ask
Topic, provides a kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method.
The present invention adopts the following technical scheme that realization：A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method,
Using following steps：A, detoxic radix rehmanniae test tube seedling on superclean bench is cut into the stem with bud of 2-2.5cm, then move into and contain
It is cultivated in sugared 30g/L, the culture medium that pH value is 5.6-6.4, cultivation temperature is 25 DEG C -28 DEG C, intensity of illumination 2500Lx-
3000Lx, light application time are 8 hours/day；B, it when detoxic radix rehmanniae test tube seedling culture 30-35 days or when growing to 10cm or more, is opening
The stem with bud for being cut into one bud of a leaf under environment with 45 degree of angles with scissors is put, and is soaked in 10-25 DEG C of tap water at once, is soaked
The time is steeped more than or equal to 2min；C, by soaked stem with bud cuttage in the seedlings nursing plate equipped with vermiculite, per two, cave band bud
Stem section, cuttage position is in diagonal linear distribution, and stem with bud, before growing sprouting, the humidity in seedlings nursing plate is 90%-95%,
Temperature is 15 DEG C -30 DEG C, and intensity of illumination is 1500Lx~2000Lx under film；D, crop field is transplanted after cuttage seeding grows sprouting and root
Complete quickly breeding.
By will again be sheared after test tube seedling shearing culture in the present invention, then the mode of cuttage and seedling culture is bred, numerous
The amount of growing is substantially improved, and uses the modes of reproduction that test tube and non-test tube are combined, and obtained detoxic seedling is more healthy and stronger, is not necessarily to
Excessively can directly transplanting, while overcoming the excessive initial stage temperature limiting of test tube seedling, overcome existing glutinous rehmannia test tube seedling breeding and deposit
In inefficient operation, problem of high cost and easy death.
Humid control is with the following method in seedlings nursing plate：Simple arched shed is built, and covers single thin film, when humidity is more than
When 95%, several apertures are pricked on film, when humidity is less than 90%, are slowly watered from arched shed edge.
Humidity control method operation is more convenient.
Breeding amount greatly improved in the present invention, compared to the whole strain transplanting of traditional test tube seedling breeding amount at 25 times or more, at
This only traditional 1/5 hereinafter, and obtained detoxic seedling it is more healthy and stronger, can directly be transplanted to crop field without excessive, while can
Normally to breed micro cuttage seedling under the outdoor temperature more than 28 DEG C or more, detoxic seedling repoductive time is substantially prolonged, further
, prevented test tube seedling by bacterium pollution or the generation of vitrification phenomenon, micro cuttage part operates in open environment, be not required to again without
Bacterium condition, it is simple and practicable, have the advantages that easy to operate, at low cost, management difficulty is small and suitable for industrial is bred.
Table 1 is that glutinous rehmannia test tube seedling culture survival rate experimental factor and level (mg/L) are had chosen using positive quadraturing design test
The optimum combination of three factors.Each tested number chooses 10 bottles of (4/bottle) detoxic radix rehmanniae test tube seedlings.
Table 1
Table 2 is that glutinous rehmannia test tube seedling culture survival rate tests each factor combination and as a result, A refers to temperature in table 2, B refers to light
According to C refers to light application time.
As can be drawn from Table 2, when highest survival rate is 0.96, cultivation temperature is 25 DEG C -28 DEG C, illumination 2500-
3000Lx, light application time are 8 hours/day.
Table 3 is glutinous rehmannia micro cuttage shoot survival percent experimental factor and level (mg/L), and being chosen using orthogonal design influences glutinous rehmannia
Three key factors of cuttage seeding, obtain the optimum combination of three factors.Each the micro cuttage seedling of 2 seedlings nursing plates of tested number selection is
Object.
When cuttage seeding density is 800-1400 plants/square metre, per one, cave in seedlings nursing plate, center cuttage；1400-
When 2000 plant/square metre, per two, cave in seedlings nursing plate, pairs of linea angulata cuttage；It is every in seedlings nursing plate when 2000-2600 plants/square metre
Three, cave, at equilateral triangle cuttage.
Table 4 is that glutinous rehmannia micro cuttage shoot survival percent tests each factor combination and as a result, A refers to humidity in table 4, B refers under film
Illumination, C refer to cutting density.
As can be drawn from Table 4, when highest survival rate is 0.98, nursery humidity is 90%-95%, under film illumination be
1500-2000Lx, it is 1400-2000 plants/square metre to refer to cutting density.
Table 5 is cost accounting.
Specific implementation mode
Embodiment 1
A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method, using following steps：It a, will on superclean bench
Detoxic radix rehmanniae test tube seedling is cut into the stem with bud of 2cm, then move into containing sugared 30g/L, pH value be 5.6 culture medium in trained
It supports, cultivation temperature is 25 DEG C, intensity of illumination 2500Lx, and light application time is 8 hours/day；B, detoxic radix rehmanniae test tube seedling culture is waited for
30 days or when growing to 10cm or more, it is cut into the stem with bud of one bud of a leaf with 45 degree of angles with scissors under open environment, and at once
It is soaked in 10 DEG C of tap water, soaking time is equal to 2min；C, by soaked stem with bud cuttage in educating equipped with vermiculite
In seed plate, per two, cave stem with bud, cuttage position in it is diagonal it is linear be distributed, and stem with bud is before growing sprouting, seedlings nursing plate
Interior humidity is 90%, and temperature is 15 DEG C, and intensity of illumination is 1500Lx under film；D, it is transplanted after cuttage seeding grows sprouting and root big
Complete quickly breeding in field.
Humid control is with the following method in seedlings nursing plate：Simple arched shed is built, and covers single thin film, when humidity is more than
When 95%, several apertures are pricked on film, when humidity is less than 90%, are slowly watered from arched shed edge.
Embodiment 2
A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method, using following steps：It a, will on superclean bench
Detoxic radix rehmanniae test tube seedling is cut into the stem with bud of 2.5cm, then move into containing sugared 30g/L, pH value be 6.4 culture medium in trained
It supports, cultivation temperature is 28 DEG C, intensity of illumination 3000Lx, and light application time is 8 hours/day；B, detoxic radix rehmanniae test tube seedling culture is waited for
35 days or when growing to 10cm or more, it is cut into the stem with bud of one bud of a leaf with 45 degree of angles with scissors under open environment, and at once
It is soaked in 25 DEG C of tap water, soaking time is equal to 8min；C, by soaked stem with bud cuttage in educating equipped with vermiculite
In seed plate, per two, cave stem with bud, cuttage position in it is diagonal it is linear be distributed, and stem with bud is before growing sprouting, seedlings nursing plate
Interior humidity is 95%, and temperature is 30 DEG C, and intensity of illumination is 2000Lx under film；D, it is transplanted after cuttage seeding grows sprouting and root big
Complete quickly breeding in field.
Humid control is with the following method in seedlings nursing plate：Simple arched shed is built, and covers single thin film, when humidity is more than
When 95%, several apertures are pricked on film, when humidity is less than 90%, are slowly watered from arched shed edge.
Embodiment 3
A kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method, using following steps：It a, will on superclean bench
Detoxic radix rehmanniae test tube seedling is cut into the stem with bud of 2.1cm, then move into containing sugared 30g/L, pH value be 5.9 culture medium in trained
It supports, cultivation temperature is 27 DEG C, intensity of illumination 2699Lx, and light application time is 8 hours/day；B, detoxic radix rehmanniae test tube seedling culture is waited for
32 days or when growing to 10cm or more, it is cut into the stem with bud of one bud of a leaf with 45 degree of angles with scissors under open environment, and at once
It is soaked in 19 DEG C of tap water, soaking time is equal to 5min；C, by soaked stem with bud cuttage in educating equipped with vermiculite
In seed plate, per two, cave stem with bud, cuttage position in it is diagonal it is linear be distributed, and stem with bud is before growing sprouting, seedlings nursing plate
Interior humidity is 92%, and temperature is 22 DEG C, and intensity of illumination is 1846Lx under film；D, it is transplanted after cuttage seeding grows sprouting and root big
Complete quickly breeding in field.
Humid control is with the following method in seedlings nursing plate：Simple arched shed is built, and covers single thin film, when humidity is more than
When 95%, several apertures are pricked on film, when humidity is less than 90%, are slowly watered from arched shed edge.
In specific implementation process, the size of seedlings nursing plate is 540 × 280 × 55mm, and the size in 32 caves, simple arched shed is 2m wide
× 10m long × 1m high, every bamboo pole or plastic tube interval 1.5m, seedling standard are that stem with bud grows sprouting 1-2 piece leaves, and length
The root of 1cm or more.
Claims (2)
1. a kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method, it is characterised in that：Using following steps：
A, detoxic radix rehmanniae test tube seedling is cut into the stem with bud of 2-2.5cm on superclean bench, then move into the 30g/L containing sugar,
It is cultivated in the culture medium that pH value is 5.6-6.4, cultivation temperature is 25 DEG C -28 DEG C, intensity of illumination 2500Lx-3000Lx,
Light application time is 8 hours/day；B, when detoxic radix rehmanniae test tube seedling culture 30-35 days or when growing to 10cm or more, under open environment
The stem with bud of one bud of a leaf is cut into 45 degree of angles with scissors, and is soaked in 10-25 DEG C of tap water at once, soaking time is big
In equal to 2min；C, by soaked stem with bud cuttage in the seedlings nursing plate equipped with vermiculite, per two, cave stem with bud, cuttage
Position is in diagonal linear distribution, and stem with bud, before growing sprouting, the humidity in seedlings nursing plate is 90%-95%, temperature 15
DEG C -30 DEG C, intensity of illumination is 1500Lx~2000Lx under film；D, crop field is transplanted after cuttage seeding grows sprouting and root to complete quickly
Breeding.
2. a kind of detoxic radix rehmanniae test tube seedling micro cuttage rapid propagation method according to claim 1, it is characterised in that：Nursery
Humid control is with the following method in disk：Simple arched shed is built, and covers single thin film, when humidity is more than 95%, in film
It is upper to prick several apertures, when humidity is less than 90%, slowly water from arched shed edge.
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| CN201810375122.7A CN108496600B (en) | 2018-04-24 | 2018-04-24 | Micro-cuttage rapid propagation method for virus-free rehmannia test-tube plantlets |
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| CN201810375122.7A CN108496600B (en) | 2018-04-24 | 2018-04-24 | Micro-cuttage rapid propagation method for virus-free rehmannia test-tube plantlets |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771504A (en) * | 2019-11-11 | 2020-02-11 | 山西省农业科学院棉花研究所 | Sugar-free culture strong seedling transplanting method for rehmannia detoxified seedlings |
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| CN1048141A (en) * | 1990-08-06 | 1991-01-02 | 天津市蔬菜研究所 | Fast propagation technique for potato using depoisoned small seed-potato |
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| CN1048141A (en) * | 1990-08-06 | 1991-01-02 | 天津市蔬菜研究所 | Fast propagation technique for potato using depoisoned small seed-potato |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110771504A (en) * | 2019-11-11 | 2020-02-11 | 山西省农业科学院棉花研究所 | Sugar-free culture strong seedling transplanting method for rehmannia detoxified seedlings |
| CN110771504B (en) * | 2019-11-11 | 2022-09-13 | 山西省农业科学院棉花研究所 | Sugar-free culture strong seedling transplanting method for rehmannia detoxified seedlings |
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Inventor after: Zuo Jingjing Inventor after: Yan Guiyun Inventor after: Huo Liguang Inventor after: Wang Huijie Inventor after: Liu Shaoxiang Inventor after: Yan Jianjun Inventor after: Zuo Min Inventor before: Zuo Jingjing |
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