CN104350913B - A kind of method for culturing seedlings of decomposite leaf Radix Rehmanniae - Google Patents
A kind of method for culturing seedlings of decomposite leaf Radix Rehmanniae Download PDFInfo
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- CN104350913B CN104350913B CN201410571999.5A CN201410571999A CN104350913B CN 104350913 B CN104350913 B CN 104350913B CN 201410571999 A CN201410571999 A CN 201410571999A CN 104350913 B CN104350913 B CN 104350913B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- Developmental Biology & Embryology (AREA)
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Abstract
The present invention relates to the method for culturing seedlings of a kind of decomposite leaf Radix Rehmanniae, including gathering seed, lamination immersion treatment and the step of transplanting.The seedling from seed survival rate and the planting percent that utilize the decomposite leaf Radix Rehmanniae that the present invention obtains are high, well developed root system.
Description
Technical field
The present invention relates to the method for culturing seedlings of decomposite leaf Radix Rehmanniae.
Background technology
Decomposite leaf Radix Rehmanniae implant is removed quilt by the long pubescence of many cells, the top tender part of children and bennet with calyx
Also by glandular hair outside above-mentioned hair.Blade papery, oblong, pinniform ftractures, not ftractureing of top,
Hua Dan raw top axil；Squamella and leaf similar shape, do not have handle, and be born in bennet base portion, corolla
Aubergine, filigree without hair or nearly base portion slightly by glandular hair.Fruit has no.The month at florescence 5-9.
It is distributed in Shaanxi, Hubei.It is born in the hillside of elevation 800-1500 rice.The side of breeding at present
Formula is mainly division propagation, and the seedling percent that seminal propagation is sprouted is relatively low, neat after emerging
Spend uneven.
Summary of the invention
The present invention is directed to existing deficiency and provide the method for culturing seedlings of a kind of decomposite leaf Radix Rehmanniae.
The present invention is realized by the following method above-mentioned purpose.
The method for culturing seedlings of a kind of decomposite leaf Radix Rehmanniae, comprises the following steps:
(1) gathering ripe decomposite leaf Radix Rehmanniae seed, physical purity of seed is 95%；
(2) seed of collection is spread in ventilation frescade, after shady dry 10-13 days, seed is first used
Mass volume ratio 0.5% potassium permanganate solution soaks 20-25min sterilization, then by the heat of 45 DEG C
Water soaking 2 hours, rinses seed with clear water after natural cooling and removes disinfectant solution residue and seed
Extractum；
(3) preparation plant lixiviating solution: choose well-developed Herba Senecionis Scandentis plant, gather its root,
Stem, leaf, shady dry after shred with shears, weigh respectively root, stem, leaf 5,2.5,10g, add
Entering 100 times of distilled water, 25～30 DEG C are soaked 48h, obtain lixiviating solution stock solution after 4 layers of filtered through gauze,
Concentrated solution is evaporated under reduced pressure to obtain with Rotary Evaporators again, is settled to 100ml；It is settled to above-mentioned
The concentrated solution of 100ml adds 20ppm gibberellins and 15ppm salicylic acid obtains lixiviating solution；
(4) lamination immersion treatment: seed is soaked in described lixiviating solution 3h, then with river sand,
Vermiculitum uniformly mixes with seed, puts in seed packet, and seed packet is put into the cultivation of 17-20 DEG C
After case is cultivated 2 days, take out seed packet, keep the skin wet and turn uniformly, then seed packet is put
Enter after the incubator of 6-8 DEG C is cultivated 3 days, further take out seed packet, keep the skin wet and turn uniformly,
Then being put into by seed packet in the incubator of 17-20 DEG C and cultivate, seed starts to show money or valuables one carries unintentionally, is i.e. used for
Sowing；
River sand: Vermiculitum: seed quality ratio=2:3:1；If turning period to be found to have mildew and rot kind
Son should be chosen in time；
(5) chitting piece is seeded on the seedbed in greenhouse, control temperature at 21-25 DEG C, Seedling
Bed substrate be to be made up of the composition of following weight portion: fertile soil 230 parts, garden mould 25 parts,
50% carbendazim 0.08 part, Vermiculitum 10 parts and river sand 30 parts, according to aforementioned proportion mixed-matrix
After, spraying substrate by after formalin dilute with water 50 times, every square metre of soil uses formal
Woods 20m1；
(6) when seedling length to 2-4cm, land for growing field crops i.e. it is transplanted to.
Beneficial effect
The invention have the advantages that
1, invention comprehensive utilization is dry, soak, growth regulator regulates and many substrate alternating temperature
Stratificated treatment etc. break the technology of seed dormant technology, particularly Stratificated treatment, including using
The multiple substrate of proper ratio and from high temperature to low temperature again to the process of high temperature so that utilize
The seedling from seed survival rate of the decomposite leaf Radix Rehmanniae that the present invention obtains and planting percent are high, well developed root system；
2, after containing Herba Senecionis Scandentis lixiviating solution and growth regulator, salicylic immersion, pole
Improve greatly the sprouting survival rate of decomposite leaf Radix Rehmanniae seed；
3, the lamination substrate that the present invention is prepared, adds the breathability of substrate, and then favorably
In the breathing of seed, therefore, the broken dormancy process of decomposite leaf Radix Rehmanniae seed is accelerated.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated, and following each embodiment is only used for
The present invention is described, the present invention is not limited.
Embodiment
The method for culturing seedlings of decomposite leaf Radix Rehmanniae, comprises the following steps:
(1) gathering ripe decomposite leaf Radix Rehmanniae seed, physical purity of seed is 95%；
(2) seed of collection is spread in ventilation frescade, after shady dry 10-13 days, seed is first used
Mass volume ratio 0.5% potassium permanganate solution soaks 20-25min sterilization, then by the heat of 45 DEG C
Water soaking 2 hours, rinses seed with clear water after natural cooling and removes disinfectant solution residue and seed
Extractum；
(3) preparation plant lixiviating solution: choose well-developed Herba Senecionis Scandentis plant, gather its root,
Stem, leaf, shady dry after shred with shears, weigh respectively root, stem, leaf 5,2.5,10g, add
Entering 100 times of distilled water, 25～30 DEG C are soaked 48h, obtain lixiviating solution stock solution after 4 layers of filtered through gauze,
Concentrated solution is evaporated under reduced pressure to obtain with Rotary Evaporators again, is settled to 100ml；It is settled to above-mentioned
The concentrated solution of 100ml adds 20ppm gibberellins and 15ppm salicylic acid obtains lixiviating solution；
(4) lamination immersion treatment: seed is soaked in described lixiviating solution 3h, then with river sand,
Vermiculitum uniformly mixes with seed, puts in seed packet, and seed packet is put into the cultivation of 17-20 DEG C
After case is cultivated 2 days, take out seed packet, keep the skin wet and turn uniformly, then seed packet is put
Enter after the incubator of 6-8 DEG C is cultivated 3 days, further take out seed packet, keep the skin wet and turn uniformly,
Then being put into by seed packet in the incubator of 17-20 DEG C and cultivate, seed starts to show money or valuables one carries unintentionally, is i.e. used for
Sowing；
River sand: Vermiculitum: seed quality ratio=2:3:1；If turning period to be found to have mildew and rot kind
Son should be chosen in time；
(5) chitting piece is seeded on the seedbed in greenhouse, control temperature at 21-25 DEG C, Seedling
Bed substrate be to be made up of the composition of following weight portion: fertile soil 230 parts, garden mould 25 parts,
50% carbendazim 0.08 part, Vermiculitum 10 parts and river sand 30 parts, according to aforementioned proportion mixed-matrix
After, spraying substrate by after formalin dilute with water 50 times, every square metre of soil uses formal
Woods 20m1；
(6) when seedling length to 2-4cm, being i.e. transplanted to land for growing field crops, now decomposite leaf Radix Rehmanniae seed becomes
Seedling rate is 92%, and transplanting survival rate is 95%.
Embodiment described above is only to be described the preferred embodiment of the present invention, and
Non-the spirit and scope of the present invention are defined.The content that the present invention is claimed all is documented in
In claims.
Claims (1)
1. the method for culturing seedlings of a decomposite leaf Radix Rehmanniae, it is characterised in that comprise the following steps:
(1) gathering ripe decomposite leaf Radix Rehmanniae seed, physical purity of seed is 95%；
(2) seed of collection is spread in ventilation frescade, after shady dry 10-13 days, seed is first used
Mass volume ratio 0.5% potassium permanganate solution soaks 20-25min sterilization, then by the heat of 45 DEG C
Water soaking 2 hours, rinses seed with clear water after natural cooling and removes disinfectant solution residue and seed
Extractum；
(3) preparation plant lixiviating solution: choose well-developed Herba Senecionis Scandentis plant, gather its root,
Stem, leaf, shady dry after shred with shears, weigh respectively root, stem, leaf 5,2.5,10g, add
Entering 100 times of distilled water, 25～30 DEG C are soaked 48h, obtain lixiviating solution stock solution after 4 layers of filtered through gauze,
Concentrated solution is evaporated under reduced pressure to obtain with Rotary Evaporators again, is settled to 100ml；It is settled to above-mentioned
The concentrated solution of 100ml adds 20ppm gibberellins and 15ppm salicylic acid obtains lixiviating solution；
(4) lamination immersion treatment: seed is soaked in described lixiviating solution 3h, then with river sand,
Vermiculitum uniformly mixes with seed, puts in seed packet, and seed packet is put into the cultivation of 17-20 DEG C
After case is cultivated 2 days, take out seed packet, keep the skin wet and turn uniformly, then seed packet is put
Enter after the incubator of 6-8 DEG C is cultivated 3 days, further take out seed packet, keep the skin wet and turn uniformly,
Then being put into by seed packet in the incubator of 17-20 DEG C and cultivate, seed starts to show money or valuables one carries unintentionally, is i.e. used for
Sowing；
River sand: Vermiculitum: seed quality ratio=2:3:1；If turning period to be found to have mildew and rot kind
Son should be chosen in time；
(5) chitting piece is seeded on the seedbed in greenhouse, control temperature at 21-25 DEG C, Seedling
Bed substrate be to be made up of the composition of following weight portion: fertile soil 230 parts, garden mould 25 parts,
50% carbendazim 0.08 part, Vermiculitum 10 parts and river sand 30 parts, according to aforementioned proportion mixed-matrix
After, spraying substrate by after formalin dilute with water 50 times, every square metre of soil uses formal
Woods 20m1；
(6) when seedling length to 2-4cm, land for growing field crops i.e. it is transplanted to.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410571999.5A CN104350913B (en) | 2014-10-23 | 2014-10-23 | A kind of method for culturing seedlings of decomposite leaf Radix Rehmanniae |
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| CN201410571999.5A CN104350913B (en) | 2014-10-23 | 2014-10-23 | A kind of method for culturing seedlings of decomposite leaf Radix Rehmanniae |
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| CN104350913A CN104350913A (en) | 2015-02-18 |
| CN104350913B true CN104350913B (en) | 2016-12-07 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105230428B (en) * | 2015-10-28 | 2018-04-10 | 甘肃康鑫苗木绿化工程有限责任公司 | A kind of lacebark pine method for culturing seedlings |
| CN105746133B (en) * | 2016-03-03 | 2019-10-01 | 李延梅 | Radix Rehmanniae High aititude mating system |
| CN107295827A (en) * | 2017-07-12 | 2017-10-27 | 李晓枫 | A kind of method for improving iris seed germination rate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85100818A (en) * | 1985-04-01 | 1986-07-09 | 武田药品工业株式会社 | A kind of breeding of new veriety of rehmannia and the method for cultivation |
| CN102301955A (en) * | 2011-07-19 | 2012-01-04 | 河南师范大学 | Method for breeding rehmannia toxin-free seedling |
| CN103348863A (en) * | 2013-07-22 | 2013-10-16 | 镇江瑞繁农艺有限公司 | Method for seed reproduction of ground cucumbers |
| CN103798038A (en) * | 2014-02-18 | 2014-05-21 | 江苏沿海地区农业科学研究所 | Full-seedling method of okra in moderate saline-alkali land of coastal mud flat |
| CN103975722A (en) * | 2014-04-25 | 2014-08-13 | 山西省农业科学院棉花研究所 | Rehmannia virus-free test tube seedling net house direct soil entering transplanting method |
-
2014
- 2014-10-23 CN CN201410571999.5A patent/CN104350913B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN85100818A (en) * | 1985-04-01 | 1986-07-09 | 武田药品工业株式会社 | A kind of breeding of new veriety of rehmannia and the method for cultivation |
| CN102301955A (en) * | 2011-07-19 | 2012-01-04 | 河南师范大学 | Method for breeding rehmannia toxin-free seedling |
| CN103348863A (en) * | 2013-07-22 | 2013-10-16 | 镇江瑞繁农艺有限公司 | Method for seed reproduction of ground cucumbers |
| CN103798038A (en) * | 2014-02-18 | 2014-05-21 | 江苏沿海地区农业科学研究所 | Full-seedling method of okra in moderate saline-alkali land of coastal mud flat |
| CN103975722A (en) * | 2014-04-25 | 2014-08-13 | 山西省农业科学院棉花研究所 | Rehmannia virus-free test tube seedling net house direct soil entering transplanting method |
Non-Patent Citations (4)
| Title |
|---|
| 千里光提取液的抗氧化活性研究与总黄酮含量测定;王如阳等;《云南中医中药杂志》;20090520;第30卷(第5期);第51-52页 * |
| 怀地黄育苗移栽试验;李茂青等;《河南农业科学》;19880131(第1期);第22-24页 * |
| 经济植物繁殖研究进展;余道平;《经济林研究》;20061230;第24卷(第4期);第87-91页 * |
| 药用植物种子生理特性及处理技术;张辰露等;《现代中药研究与实践》;20050228;第19卷(第1期);第59-64页 * |
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Effective date of registration: 20160920 Address after: 226131 Jiangsu city of Nantong Province Haimen Yuelai town Fuxing Road, building 12, the town government compound Applicant after: Nantong Yue Yue Industrial Co., Ltd. Address before: 545000 the Guangxi Zhuang Autonomous Region District of Liuzhou City Road 2 Eshan Liunan District 57. Applicant before: Zhou Qi |
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