CN105850683A - Long-shoot cutting breeding method of tea tree shoots - Google Patents
Long-shoot cutting breeding method of tea tree shoots Download PDFInfo
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- CN105850683A CN105850683A CN201610259665.3A CN201610259665A CN105850683A CN 105850683 A CN105850683 A CN 105850683A CN 201610259665 A CN201610259665 A CN 201610259665A CN 105850683 A CN105850683 A CN 105850683A
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- VZJVWSHVAAUDKD-UHFFFAOYSA-N Potassium permanganate Chemical compound [K+].[O-][Mn](=O)(=O)=O VZJVWSHVAAUDKD-UHFFFAOYSA-N 0.000 claims description 6
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- 230000003442 weekly Effects 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 4
- 229920001542 oligosaccharide Polymers 0.000 claims description 4
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- 239000005739 Bordeaux mixture Substances 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N Carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- YWTYJOPNNQFBPC-UHFFFAOYSA-N Imidacloprid Chemical compound [O-][N+](=O)\N=C1/NCCN1CC1=CC=C(Cl)N=C1 YWTYJOPNNQFBPC-UHFFFAOYSA-N 0.000 claims description 3
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
Abstract
The invention discloses a long-shoot cutting breeding method of tea tree shoots. The method comprises steps as follows: shoot picking, water loss treatment of shoots, shoot shearing, rooting protection fluid treatment, disinfection treatment, cuttage and management after cuttage, wherein the rooting protection fluid treatment step is implemented as follows: indolebutyric acid with the concentration being 150-250 mg/kg and an oligosaccharin mixed solution with the concentration being 100-150 mg/kg are adopted for soaking the root parts of shoots for cuttage for 3-5 h. The long-shoot cutting breeding method has the advantages of rapidness in rooting, high survival rate, short seedling culture period, high propagation coefficient, low production cost, convenience in management and the like.
Description
Technical field
The invention belongs to technical field of plant asexual propagation, relate to the mating system of a kind of tea tree spray long fringe cuttage.
Background technology
Ling Yaoxing belongs to green tea, original name " raw white tea ", has another name called " raw white tea of reaching the clouds ", gains the name because its blade back covers with pekoe.
In the Yunwu Mountain that main product reaches the clouds in Guangxi Zhuang Autonomous Region, work in peace and contentment in two borders of the county, with the beautiful flood of Qinglongshan one band, add outstanding two places
Pekoe tea performance optimal, yield is most.Element is with look emerald green, milli is many, fragrant height, the big characteristic of taste bubble dense, resistance to five are well-known at home and abroad, becomes
Rising star in Chinese famous tea.
Tea tree " osmanthus heat No. 2 " be Guangxi South Asian Tropical Agricultural Science Research Institute scientific and technical personnel from Ling Yaoxing colony plants through list
The improved seeds that strain selects, carried out cultivar registration in 2006, within 2008, carried out kind grafting demonstration in district.This kind
Mobile jib is obvious, tree performance half, and it is oblique that leaf raw upper half, belongs to arbor type large leaf, and bud head is stout and strong, and fine hair spy is many, leaf look pale green,
Suitable excellent green tea processed, black tea etc..
Along with the development of China's tealeaves, tea tree improved Varieties demand is strengthened.Currently my tea place, district breed structure is the most single,
Breeding popularity rate and Famous High-quality Tea ratio are low, and tea products structure can not meeting the market requirement.In conjunction with supporting cottage method to tea
Tree osmanthus heat 2 carries out vegetative propagation and passes on for the osmanthus heat guarantor of No. 2 is excellent, accelerates stock breeding speed significant.
Summary of the invention
The technical problem to be solved in the present invention is to provide the mating system of a kind of tea tree spray long fringe cuttage, makes tea tree osmanthus heat 2
Good characteristic is preserved.
The present invention is by the following technical solutions:
The mating system of a kind of tea tree spray long fringe cuttage, comprises the following steps:
(1) fringe is adopted: choose raw tender without the green of pest and disease damage, robust growth then from the top of osmanthus No. 2 choiceness tea trees of heat
Branch, standby；
(2) dehydration of fringe bar processed: by In Shade 3-4 days of the fringe bar adopted back, and watered and keep fringe bar fresh and alive；
(3) special cuttings is made: cuttings retains 2-4 blade, and cuttings bottom at least retains two internodes, long 5-8cm；On
Clip straight snips, clip is away from joint 1-2cm；Lower clip is tiltedly cut, clip direction with nethermost blade towards consistent；
(4) cuttings is processed with protection liquid of taking root: with the indolebutyric acid that concentration is 150-250mg/kg and the ammonia of 100-150mg/kg
Base oligosaccharide mixed solution soaks cuttings base portion 3-5 hour；
(5) the disinfecting of cuttings: before cuttage, cuttings base portion is immersed in 4-6 in the carbendazim liquid of 800-1000 times of liquid
Minute；
(6) test slotting machine: build seedbed, bottom, seedbed is provided with osculum, the cobblestone that bed bottom paving 15-20 is thick, and upper strata spreads
15-20 thickness press 3:1 mass ratio mixing river sand and perlite as cutting medium；Within before cuttage 2-3 days, arrange slotting machine, use
0.1-0.15% potassium permanganate spray disinfectant, airing；
(7) cuttage: carry out cuttage before at 10 in Southwest Guangxi area 5-12 morning in month or after at 4 in afternoon, high to avoid noon
The temperature period；
(8) cottage method: use straight cutting method, cuttings be inserted directly in matrix, then compresses by real, and the degree of depth of cuttage is
5-8cm。
(9) manage after inserting:
1. 50-60% Shading net covering is used after cuttage；
2. the control of spray time: cuttage fringe is carried out spraying management, make blade face keep water membrane, Callus formation it
Before on blade water film evaporation be reduced to when 1/3 to start spraying；After Callus formation, after blade face moisture film evaporates completely again
Spray；Root growth and length, when more than 3cm, are sprayed at noon；
3. fertilising: starting to topdress after cuttage seeding forms root system, early stage sprays weekly the potassium dihydrogen phosphate of 0.2-0.3%, treats
When root system grows to more than 5cm, the gibberellin of additional 180-220mg/kg 3-5 time, accelerate cuttage seeding rudiment and young sprout growth, after
Phase sprays weekly the potassium dihydrogen phosphate of 0.4-0.5%, and every first quarter moon imposes the urea of a 0.3-0.4%；
4. hardening: gradually open sunshade net after taking root 30 days and carry out hardening, take off net for the first time, the method the most entirely taken off after taking partly to take off
Carry out hardening, progressively strengthen the survival rate after adaptation ability and the resistance of tea shoot, nurturing staff and raising are transplanted；
5. weeding, except bud: pull out the weeds on seedbed；Extract the bud occurred in cuttings, to ensure to nourish and grow institute
Need the supply of nutrient；
6. the prevention and control of plant diseases, pest control: sprayed once half amount formula Bordeaux mixture after cuttage every 15-30 days, control generation and the development of leaf disease；
Spray Biphenthrin or imidacloprid medicament carries out pest control；
(10) tea shoot goes out garden.
In described step (7), cutting time is preferably September.
The present invention have take root rapidly, survival rate is high, growing-seedling period is short, breeding coefficient is high, production cost is low and is easy to management etc.
Advantage.
Detailed description of the invention
Below in conjunction with test example, technical scheme and beneficial effect are described further.Hundred the present invention relates to
Proportion by subtraction content, in addition to Special Statement, all referring to mass percentage content.
1. fringe bar pre-treatment: from osmanthus heat No. 2 choiceness tea trees top choose then give birth to without pest and disease damage, robust growth green
Look spray, In Shade 5 days, and the holding fringe bar that waters is fresh and alive；Cuttings retains three leaves, and cuttings bottom retains two internodes,
Long 7-8cm；Upper clip straight snips, clip is away from joint 1cm；Lower clip is tiltedly cut, clip direction with nethermost blade towards consistent.
2. prepare protection liquid of taking root: being carried out according to the following table the preparation of protection 17 samples of liquid A-Q of taking root.(unit: mg/kg)
| Formula | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q |
| Indolebutyric acid | 150 | 150 | 250 | 250 | 200 | 200 | 200 | 150 | 250 | 150 | 150 | 250 | 300 | 300 | 300 | 0 | 200 |
| Amino-oligosaccharide | 100 | 150 | 100 | 150 | 100 | 150 | 120 | 120 | 120 | 50 | 100 | 200 | 100 | 150 | 200 | 100 | 0 |
3. test is arranged: above-mentioned 17 protection liquid of taking root are plus 1 clear water comparison, 18 process altogether, each process 5
Repeat, 20 fringe bars of each repetition.
4. test method: fringe bar base portion is inserted respectively after soaking 4 hours in above-mentioned 18 solution processed, according to lower section
Method carry out unifying cuttage and unified insert after manage:
Before cuttage, cuttings base portion is immersed in the carbendazim liquid of 1000 times of liquid 5 minutes.
Cuttage: a height of 0.5m being built into common brick, a width of 1.3m, the rectangle seedbed of a length of 16m, bottom is provided with osculum,
Bed bottom spreads the cobblestone of 20 thickness, the thick pure river sand pressing the mixing of 3:1 mass ratio of upper strata paving 15 and perlite conduct
Cutting medium.First 3 days arrangement slotting machines of cuttage, with 0.1% potassium permanganate spray disinfectant, airing.Select the morning 10 in September
Before Dian, after at 4 in afternoon, carry out cuttage.Cuttage uses straight cutting method, by the seeding row spacing of 4cm × 10cm, cuttings is inserted directly into base
In matter, then compressing by real, the degree of depth of cuttage is 5-8cm.
Manage after Chaing:
1. 50-60% Shading net covering is used after inserting, rainproof, windproof, sun-proof, it is beneficial to take root.
2. the control of spray time: in cuttage in early days, before Callus formation, many sprays, make blade face often keep water membrane,
Treat on blade that water film evaporation is reduced to 1/3 and starts spraying；After Callus formation, to suitably reduce spraying, at blade face moisture film
Wait a moment after evaporation completely and spray again；After a large amount of root growths (more than 3cm), can only spray the most on a small quantity,
But matrix need to periodically be watered.
3. fertilising: use duty execute thin execute and and optimize fertilization mode, after cuttage seeding preliminarily forms root system, start to topdress, front
Phase sprays weekly the potassium dihydrogen phosphate of 0.2%, when root system grows to 5cm, and the gibberellin of additional 200mg/kg 3-5 time,
Accelerating cuttage seeding rudiment and young sprout growth, the later stage sprays weekly the potassium dihydrogen phosphate of 0.4%, and every first quarter moon imposes one time 0.3%
Urea.
4. hardening: gradually opening sunshade net after taking root 30 days and carry out hardening, take off net for the first time, the method the most entirely taken off after taking partly to take off is entered
Row hardening, progressively strengthens the survival rate after adaptation ability and the resistance of tea shoot, nurturing staff and raising are transplanted.
5. weeding, except bud: the weeds on seedbed to be pulled out at any time, accomplish except little, eliminate；The bud occurred in cuttings,
To extract in time, the supply of required nutrient of nourishing and growing with guarantee.
6. the prevention and control of plant diseases, pest control: sprayed once half amount formula Bordeaux mixture after cuttage every 15-30 days, control generation and the development of leaf disease.
Pest control can spray 10% imidacloprid mu 15-20 gram.
5. result investigation:
1 month " Invest, Then Investigate " rooting rate, 2 months " Invest, Then Investigate " root of hair quantity, root length and survival rate.Statistics when tea shoot goes out garden after 8 months
Outplanting rate.
Cuttings total strain number/cuttings total strain number * 100% of rooting rate=take root
Cuttings total strain number/cuttings total strain number * 100% of survival rate=survive
Cuttings total strain number of the total quantity of the root bar of root of hair quantity=take root in cuttings/take root.
Root length refers to the length of root bar, takes the mean value of each process.
Result of the test statistics is as shown in the table:
As can be known from the above table, fringe bar after protection liquid A-I of taking root processes, rooting rate, root of hair quantity, root length, survival rate and go out garden
Rate all significantly improves than take root protection liquid P, Q；After protection liquid J-O of taking root processes, each index all improves inconspicuous, even goes out
Show inhibitory action.Therefore, present invention determine that take root protection liquid formula be: concentration be 150-250mg/kg indolebutyric acid and
The amino-oligosaccharide mixed solution of 100-150mg/kg.
Claims (3)
1. the mating system of a tea tree spray long fringe cuttage, it is characterised in that comprise the following steps: adopt at fringe, fringe bar dehydration
Reason, cut fringe, protection liquid of taking root processes, disinfects, cuttage and management after inserting；Described protection liquid of taking root processes step and is:
Cuttings base portion 3-5 is soaked with the amino-oligosaccharide mixed solution of the indolebutyric acid that concentration is 150-250mg/kg and 100-150mg/kg
Hour.
The mating system of tea tree spray the most according to claim 1 long fringe cuttage, it is characterised in that specifically comprise the following steps that
(1) fringe is adopted: choose from the top of choiceness tea tree and give birth to without pest and disease damage, the green spray of robust growth then, standby；
(2) dehydration of fringe bar processed: by In Shade 3-4 days of the fringe bar adopted back, and watered and keep fringe bar fresh and alive；
(3) fringe is cut: cuttings retains 2-4 blade, and cuttings bottom at least retains two internodes, long 5-8cm；Upper clip straight snips,
Clip is away from joint 1-2cm；Lower clip is tiltedly cut, clip direction with nethermost blade towards consistent；
(4) cuttings is processed with protection liquid of taking root；
(5) disinfect: before cuttage, cuttings base portion is immersed in the carbendazim liquid of 800-1000 times of liquid 4-6 minute；
(6) cuttage: build seedbed, bottom, seedbed is provided with osculum, the cobblestone that bed bottom paving 15-20 is thick, upper strata paving 15-20
The thick river sand pressing the mixing of 3:1 mass ratio and perlite are as cutting medium；Within before cuttage 2-3 days, arrange slotting machine, use 0.1-0.15%
Potassium permanganate spray disinfectant, airing；Cuttage is carried out before at 10 in the morning in Southwest Guangxi area 5-12 month or after at 4 in afternoon,
To avoid the high temperature period at noon；Cuttage uses straight cutting method, cuttings is inserted directly in matrix, then compresses by real, cuttage deep
Degree is 5-8cm；
(7) manage after inserting:
1. 50-60% Shading net covering is used after cuttage；
2. the control of spray time: cuttage fringe is carried out spraying management, make blade face keep water membrane, Callus formation it
Before on blade water film evaporation be reduced to when 1/3 to start spraying；After Callus formation, after blade face moisture film evaporates completely again
Spray；Root growth and length, when more than 3cm, are sprayed at noon；
3. fertilising: starting to topdress after cuttage seeding forms root system, early stage sprays weekly the potassium dihydrogen phosphate of 0.2-0.3%, treats
When root system grows to more than 5cm, the gibberellin of additional 180-220mg/kg 3-5 time, accelerate cuttage seeding rudiment and young sprout growth, after
Phase sprays weekly the potassium dihydrogen phosphate of 0.4-0.5%, and every first quarter moon imposes the urea of a 0.3-0.4%；
4. hardening: gradually open sunshade net after taking root 30 days and carry out hardening, take off net for the first time, the method the most entirely taken off after taking partly to take off
Carry out hardening, progressively strengthen the survival rate after adaptation ability and the resistance of tea shoot, nurturing staff and raising are transplanted；
5. weeding, except bud: pull out the weeds on seedbed；Extract the bud occurred in cuttings, to ensure to nourish and grow institute
Need the supply of nutrient；
6. the prevention and control of plant diseases, pest control: sprayed once half amount formula Bordeaux mixture after cuttage every 15-30 days, control generation and the development of leaf disease；
Spray Biphenthrin or imidacloprid medicament carries out pest control.
The mating system of tea tree spray the most according to claim 2 long fringe cuttage, it is characterised in that described step (6)
In, before at 10 in the morning that cutting time is Southwest Guangxi area September or after at 4 in afternoon.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106106003A (en) * | 2016-08-18 | 2016-11-16 | 广西壮族自治区桂林茶叶科学研究所 | A kind of mating system of YESHENG CHA nursery stock |
| CN106386353A (en) * | 2016-09-14 | 2017-02-15 | 广西壮族自治区林业科学研究院 | Seedling growing method of tender branch cutting for keteleeria cyclolepis flous |
| CN107242130A (en) * | 2017-08-09 | 2017-10-13 | 信阳祥云茶业有限公司 | Clonal cultivar method for culturing seedlings |
| CN108283108A (en) * | 2018-01-26 | 2018-07-17 | 福建省亚热带植物研究所 | A kind of mappia foetida cuttage and seedling culture method |
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| CN113575305A (en) * | 2021-05-24 | 2021-11-02 | 河北桃晟堂农业科技开发有限公司 | Seed seedling raising method for effectively promoting growth of dandelion |
| CN114175930A (en) * | 2021-12-29 | 2022-03-15 | 南京农业大学 | Efficient rapid propagation method for accelerating growth of tea seedlings |
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