CN117581871B

CN117581871B - Composite plant growth regulator for tea plum cutting and tea plum cutting method

Info

Publication number: CN117581871B
Application number: CN202410081520.3A
Authority: CN
Inventors: 林金星; 张莹莹; 张贵芳; 张曦; 李晓娟; 胡洪飞; 张妍; 李琴; 陈亚立; 付凯
Original assignee: Nanchang Innovation Research Institute Of Peking University; Beijing Forestry University
Current assignee: Nanchang Innovation Research Institute Of Peking University; Beijing Forestry University
Priority date: 2024-01-19
Filing date: 2024-01-19
Publication date: 2024-05-17
Anticipated expiration: 2044-01-19
Also published as: CN117581871A

Abstract

The invention provides a composite plant growth regulator for tea plum cuttage and a cuttage method of tea plums. A compound plant growth regulator for cutting tea tree comprises a composition A and a composition B; the composition A comprises methyl anthranilate and indolebutyric acid, wherein the mass ratio of the methyl anthranilate to the indolebutyric acid is 7.5-151:100-1000; the composition B comprises uniconazole. The invention effectively shortens the cutting rooting time of the tea plums by utilizing the synergistic effect of the three components, and greatly increases the rooting quantity and quality.

Description

Composite plant growth regulator for tea plum cutting and tea plum cutting method

Technical Field

The application relates to the field of plant growth regulators, in particular to a composite plant growth regulator for tea plum cuttage and a cuttage method of tea plum.

Background

The tea plum is a evergreen flower shrub or small arbor, belongs to camellia genus of camellia family, and has the characteristics of plum blossom and camellia because of its flower shape, beautiful tree form, luxuriant flower and leaf, strong stress resistance, long flowering period from winter to early spring, magnificent flowers and large quantity of flowers. The tea plum is used for embellishment Cheng Jing in landscaping, is prepared into flower bed and flower mirror, has good effect, can be potted in daily life, and is placed at the positions of study rooms, meeting places, halls, door edges, windowsill and the like, and is added with fun and wonderful colors. Along with the improvement of the physical living standard of people, the requirements on community greening and living environment beautifying are also more and more exquisite, and the tea plums are used as flower shrubs loved by people, so that the demand of the tea plums in bonsai and garden greening markets is increased increasingly, and the high-quality varieties of the tea plums are greatly important to be increased and propagated rapidly. The cutting propagation can stabilize the excellent characters of the inherited parents and realize rapid mass propagation, and the method is simple and low in cost, and is an effective method for propagation of improved varieties of trees and flowers in production. Therefore, the research of a method for rapidly propagating the fine variety of the tea plum has important practical significance. However, the current tea plum cuttage has the problems of low rooting rate and survival rate of cuttings, slow rooting and the like. At present, some rooting agents on the market mostly take auxin as a single effective component, and have little effect of promoting cutting seedling raising of tea plums.

For this purpose, the present invention is proposed.

Disclosure of Invention

The invention mainly aims to provide a composite plant growth regulator for tea plum cuttage and a cuttage method of tea plums, wherein the regulator utilizes the synergistic effect of three components to effectively shorten the rooting time during tea plum cuttage, improve the survival rate and greatly increase the rooting quantity and quality.

In order to achieve the above object, the present invention provides the following technical solutions.

The first aspect of the invention provides a compound plant growth regulator for tea plum cutting, comprising a composition A and a composition B;

The composition A comprises methyl anthranilate and indolebutyric acid, wherein the mass ratio of the methyl anthranilate to the indolebutyric acid is 7.5-151:100-1000; the composition B comprises uniconazole.

Therefore, the invention takes the methyl anthranilate, the indolebutyric acid and the uniconazole as the effective components, has pertinency to the cuttage of the tea plum, and can achieve the following effects: in the initial stage of rooting, the addition of the methyl anthranilate can effectively promote the synthesis of indoleacetic acid in the tea plum body, and the synthesis of indoleacetic acid and the combined action of the indoleacetic acid can rapidly promote the content of auxin in tea plum cuttings, promote the formation of adventitious roots, shorten the rooting time, and effectively increase the rooting quantity and the rooting length; in the later rooting period, uniconazole is sprayed on leaf surfaces, so that the balance of overground and underground parts can be effectively adjusted, and the growth quality and growth speed of root systems are improved. In a word, the invention utilizes the synergy of the methyl anthranilate, the indolebutyric acid and the uniconazole to play a role of 1+1+1> 3.

The compositions A and B in the composite plant growth regulator of the invention can only contain the active ingredients, and can also form a stable formulation with auxiliary agents.

Further, the adhesive further comprises an auxiliary agent, wherein the auxiliary agent comprises at least one of a binder, a filler, a solvent and a stabilizer.

The auxiliary agents are favorable for preparing the active ingredients into a preparation form with good stability and favorable absorption by plants.

The binder may be one or more of clay, bentonite, montmorillonite, attapulgite and kaolin, gypsum, cellulose, titanium dioxide, zinc oxide, starch, dextrin, sugar (e.g. lactose, sucrose), silica, talc, mica, diatomaceous earth, urea, etc. These binders help to stably bind the active ingredient to the root/basal surface of the plant, and to prolong the contact time, thereby improving the efficacy.

The solvent can be a component which can uniformly disperse the active ingredient and has little or no damage to the plant rhizome.

Further, the binder includes clay.

Further, the solvent includes water.

Further, the composition A is powder or water aqua; and/or the composition B is powder or water aqua. In general, when preparing the aqueous solution, the active ingredient is dissolved in a small amount of organic solvents such as absolute ethyl alcohol, methanol and the like, and then diluted with water to fix the volume.

Further, the composition B is an aqueous agent, and the concentration of the uniconazole in the composition B is 100-500mg/L. The synergy is more remarkable after the medicament with the concentration is used.

Further, the composition A is an aqueous agent, the concentration of the methyl anthranilate in the composition A is 4.5-760 mg/L, and the concentration of the indolebutyric acid is 100-1000 mg/L. The synergistic effect is more remarkable after the medicament with the concentration is used, and the medicament has more pertinency to the specific plant of the tea plum.

Further, the mass ratio of the methyl anthranilate to the indolebutyric acid is 7.5-151:100-1000. The synergistic effect of the two components is more remarkable, and the tea plum fruit tea has more pertinence to the specific plant of the tea plum.

The second aspect of the invention provides a cutting method of tea plums, comprising the following steps:

Soaking cutting of tea tree in solution containing composition A in the composite plant growth regulator of the first aspect, taking out, inserting into matrix, culturing to grow adventitious root, and spraying Shi Handi solution containing composition B in the composite plant growth regulator of the first aspect onto leaf surface.

The solution containing composition A or composition B refers to a solution system. When the compositions A and B are powders, the compositions A and B may be dissolved or dispersed by adding a solvent such as water (usually, the compositions A and B are dissolved in a small amount of an organic solvent and diluted with a large amount of water). In the case of aqueous formulations, the aqueous formulations may be used as they are, with or without dilution.

The cuttings are usually obtained by the following means: the method comprises the steps of cutting the current-year semi-lignified branches which are free of plant diseases and insect pests and grow well, trimming the branches into cuttings with the length of 3-4 cm and the axillary buds of 1-2, carrying out flush cutting on the top end incision and oblique cutting on the base incision, reserving 1 blade below the axillary buds at the top, removing the rest, and then immersing the base of the cuttings into disinfectant for disinfection.

Further, the matrix comprises loess and river sand, and the volume ratio of the loess to the river sand is 3.5-4.5:0.5-1.5. After loess and river sand are mixed according to the volume ratio, the plum tea has proper air permeability and water retention property, and is more suitable for the growth characteristics of the plum tea.

Further, the spraying of the solution containing the composition B onto the leaf surface includes: spraying once every 7-10 d, and totally spraying 2-3 times.

Further, the soaking time is 1-4 hours;

and/or the soaking depth is 1-2 cm;

and/or the depth of the insertion matrix is 1/3-2/3 of the length of the cutting slips.

Further, the plastic film can be covered at the initial stage for heat preservation and moisture preservation during the culture, and the plastic film can be removed at the later stage. For example, when about 50% of cutting slips grow adventitious roots during batch cutting, the plastic film can be removed.

Furthermore, the cutting time of the cutting method is 6-9 months per year, and the effect is better.

Further, the substrate can be infiltrated and sterilized with 0.5% potassium permanganate solution prior to cutting.

In conclusion, compared with the prior art, the invention achieves the following technical effects:

(1) Different components in the composite plant growth regulator are orderly added in different growth periods of the tea tree, so that the synergistic effect of three components of methyl anthranilate, indolebutyric acid and uniconazole can be exerted, the rooting time is shortened, the number and the length of root systems are increased, the rooting rate reaches about 94.2% after 90 d of cuttage, the seedling raising cost is greatly reduced, and a foundation is laid for large-scale cuttage propagation of the tea tree.

(2) The culture condition and the appearance characteristics of the cutting and the like of the tea tree during cutting are optimized, so that the rooting efficiency is further improved, and the rooting quality is improved.

The foregoing description is only an overview of the present application, and is intended to be implemented in accordance with the teachings of the present application in order that the same may be more clearly understood and to make the same and other objects, features and advantages of the present application more readily apparent.

Drawings

Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the application. Also, like reference numerals are used to designate like parts throughout the accompanying drawings. In the drawings:

FIG. 1 is a photograph of a cutting bed used in the test of the present invention;

FIG. 2 is a growth chart of the cutting seedlings of the tea plums;

FIG. 3 shows rooting results after tea tree in each example of the present invention and comparative example.

Detailed Description

Embodiments of the technical scheme of the present application will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present application, and thus are merely examples, and are not intended to limit the scope of the present application.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description of the application and the claims and the description of the drawings above are intended to cover a non-exclusive inclusion.

In the description of the embodiments of the present application, the meaning of "plurality" is two or more unless explicitly defined otherwise.

Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.

Unless otherwise specified in the following examples and comparative examples, "ANT" means methyl anthranilate and "IBA" means indolebutyric acid. The uniconazole is uniconazole original drug of Solebao.

Example 1

A compound plant growth regulator for cutting tea tree comprises composition A and composition B.

Composition A included methyl anthranilate and indolebutyric acid in a mass ratio of 7.5:300. Firstly, weighing indolebutyric acid powder, and completely dissolving the indolebutyric acid powder with a small amount of absolute ethyl alcohol to obtain a mixed solution 1. Then, a pipette is used for sucking the o-amino and dissolving the o-amino into a small amount of absolute ethyl alcohol by methyl formate to obtain a mixed solution 2, the mixed solution 1 and the mixed solution 2 are mixed together, water is added to fix the volume to 1L, and the composition A is prepared, wherein the concentration of the o-amino methyl benzoate and the concentration of the indolebutyric acid are respectively 7.5 mg/L and 300 mg/L.

Composition B includes uniconazole. Weighing uniconazole powder, completely dissolving with a small amount of methanol, adding water to a volume of 1L, and preparing a composition B, wherein the concentration of uniconazole is 150mg/L.

Example 2

Composition A comprises methyl anthranilate and indolebutyric acid, wherein the mass ratio of the methyl anthranilate to the indolebutyric acid is 151:300. Firstly, weighing indolebutyric acid powder, and completely dissolving the indolebutyric acid powder with a small amount of absolute ethyl alcohol to obtain a mixed solution 1. Then, a pipetting gun is used for sucking the o-amino and dissolving the o-amino into a small amount of absolute ethyl alcohol by methyl formate to obtain a mixed solution 2, the mixed solution 1 and the mixed solution 2 are mixed together, water is added to fix the volume to 1L, and the composition A is prepared, wherein the concentration of the o-amino methyl benzoate and the concentration of the indolebutyric acid are 151 mg/L and 300 mg/L respectively.

Comparative example 1

And (5) clean water.

Comparative example 2

The Guoguang rooting powder (20% naphthylacetic acid powder) is diluted by 800 times of liquid, 1.2g is weighed, and water is added for dilution to 1L.

Comparative example 3

Indolebutyric acid 300mg/L: the indolebutyric acid was weighed into a beaker, dissolved in 5ml of absolute ethanol, and diluted to 1L with water.

Comparative example 4

A composite plant growth regulator comprising only composition a.

The above examples and comparative examples were used for cutting of camellia sinensis, in particular as follows.

The test steps are as follows:

step 1, preparing a cutting seedbed and sterilizing

Loess and river sand are mixed according to the volume ratio of 4:1 are mixed and prepared, a cutting seedling bed of 5m multiplied by 1.2 multiplied by m multiplied by 10cm is paved, and a sunshade with the shading rate of 75 percent is built above the seedling bed. The seedbed was sterilized by infiltration with 0.5% potassium permanganate solution 1 day before cutting and covered with plastic film.

Step 2, preparing cutting cuttings

Before 9 am, shearing 2-year-old semi-lignified branches which are free of plant diseases and insect pests and strong in growth, trimming the branches into cuttings with the length of 3-4 cm and the length of 1-2 axillary buds, carrying out flat cutting on the top end incision and oblique cutting on the base incision, retaining 1 leaf below the topmost axillary bud, removing the rest, and then immersing the base of the cuttings into 50% carbendazim of 800 times liquid for disinfection. 120 slips were processed per treatment.

Step 3, rooting agent treatment of cuttings

(1) Rooting agents were prepared according to the preparation methods in examples and comparative examples.

(3) The cutting bases 1-2 cm prepared in step 2 were immersed in the above-formulated composition a (or the solution formulated in the comparative example) for immersion 2 h.

Step4, cutting

Inserting the cutting after the soaking in the step 3 into the seedling bed prepared in the step 1, wherein the insertion depth is 2/3 of the length of the cutting, watering thoroughly at one time, inserting iron wires at two sides of the seedling bed, covering the seedling bed with a plastic film, and building a plastic small arch shed with the height of about 50 cm on the seedling bed.

Step 5, post-cutting management

(1) And (3) water management: judging whether water is deficient according to dew covering condition on the film arch shed, if water is deficient, watering in grooves at two sides of the seedbed, and supplementing water.

(2) And (3) nutrition management: after about 50% of the cuttings grow adventitious roots, the film is uncovered, the heads on two sides are uncovered first, and after two or three days, the film is uncovered completely. Composition B (comparative example no B, with clear water instead) formulated in step 3 was sprayed on the leaf surfaces of the cuttings, once every 7-10 d sprays, 2-3 sprays in succession.

(4) And (5) observing rooting conditions after cutting 40 d.

(5) Survival and rooting conditions are counted after 90 d of cutting.

Step 6, data statistics and analysis

Washing off root matrixes after cutting 40 d, counting the number of adventitious roots generated by cutting, and measuring the length of the adventitious roots of the cutting by using a ruler;

average adventitious root number = number of adventitious roots/total measured cutting shoot number

Average length of adventitious roots = length of each adventitious root/total measured adventitious root number

Survival rate = (number of survival branches/total number of cutting branches) ×100%

Rooting rate= (number of rooting branches/total number of cutting branches) ×100%

Analysis of results

The results are shown in Table 1 and FIG. 3. Cutting treated in comparative example 1 was still in callus state when cutting 40 d; the cuttings treated in comparative examples 2 and 3 produced a small number of adventitious roots, the average number of adventitious roots was 6 and 6.7, respectively, and the average lengths of adventitious roots was 1.7 cm and 1.2 cm, respectively; examples 1 and 2 produced a large number of adventitious roots, with average numbers of adventitious roots of 11.8 and 17.6, respectively, and average lengths of 1.9 cm and 2.6 cm, respectively. The above results indicate that the time for the example cuttings to begin rooting, the number and length of adventitious roots produced are significantly better than the comparative example. And the effect of example 2 is more superior to that of example 1. In addition, the rooting rate of example 2 reached 94.2% at 90 d by cutting. Therefore, the rooting agent and the cutting seedling method composed of the compositions A and B solve the problems of low cutting rooting rate, low survival rate, slow rooting and the like of the tea plums. Compared with the existing commercial rooting agent, the rooting agent not only shortens the rooting time and improves the number and length of root systems, but also ensures that the rooting rate reaches about 94.2% after 90 d of cuttage, greatly reduces the seedling cost and lays a foundation for large-scale cuttage propagation of the tea plums.

TABLE 1 average rooting number and root length statistics for 40 d cuttings

Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the application has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the application, and are intended to be included within the scope of the appended claims and description. In particular, the technical features mentioned in the respective embodiments may be combined in any manner as long as there is no structural conflict. The present application is not limited to the specific embodiments disclosed herein, but encompasses all technical solutions falling within the scope of the claims.

Claims

1. A compound plant growth regulator for tea plum cutting, which is characterized by comprising a composition A and a composition B;

The effective component of the composition A consists of methyl anthranilate and indolebutyric acid, wherein the mass ratio of the methyl anthranilate to the indolebutyric acid is 7.5-151:100-1000; the composition B comprises uniconazole.

2. The composite plant growth regulator according to claim 1, wherein the composition a is a powder or an aqueous agent.

3. The composite plant growth regulator according to claim 1, wherein the composition B is a powder or an aqueous agent.

4. The compound plant growth regulator according to claim 2, wherein the composition a is an aqueous solution, and the concentration of methyl anthranilate in the composition a is 4.5 to 760mg/L, and the concentration of indolebutyric acid is 100 to 1000mg/L.

5. A composite plant growth regulator according to claim 3, wherein composition B is an aqueous solution and the concentration of uniconazole in composition B is 100-500mg/L.

6. The compound plant growth regulator according to claim 5, wherein the concentration of uniconazole in the composition B in the aqueous solution is 100 to 150 mg/L.

7. A cutting method of tea plums, which is characterized by comprising the following steps:

soaking the cutting of the tea tree in the solution containing the composition A in the compound plant growth regulator of any one of claims 1-6, taking out and inserting the matrix, culturing until adventitious roots grow, and spraying the solution containing the composition B in the compound plant growth regulator of any one of claims 1-6 on leaf surfaces.

8. The cutting method according to claim 7, wherein the substrate comprises loess and river sand in a volume ratio of 3.5 to 4.5:0.5-1.5.

9. The cutting method according to claim 7 or 8, wherein spraying the solution containing composition B onto the foliage comprises: spraying once every 7-10 d, and totally spraying 2-3 times.

10. The cutting method according to claim 7, wherein the soaking time is 1-4 hours;

and/or the soaking depth is 1-2 cm;