CN107568227A - A kind of auxin and application method that can improve camellia azalea cuttage survival rate - Google Patents

A kind of auxin and application method that can improve camellia azalea cuttage survival rate Download PDF

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CN107568227A
CN107568227A CN201710956822.0A CN201710956822A CN107568227A CN 107568227 A CN107568227 A CN 107568227A CN 201710956822 A CN201710956822 A CN 201710956822A CN 107568227 A CN107568227 A CN 107568227A
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auxin
survival rate
naa
iba
camellia azalea
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薛克娜
玄祖迎
田雪琴
柯欢
赵鸿杰
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Foshan University
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Foshan University
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Abstract

The present invention relates to a kind of auxin and application method that can improve camellia azalea cuttage survival rate.Growth hormone includes methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA), and the mixed volume ratio of the methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA) is 1:4.The concentration of growth hormone combination is 0.50g/L.The invention enables the cuttage survival rate of camellia azalea cuttings to be greatly improved, and accelerates the speed of growth.

Description

A kind of auxin that can improve camellia azalea cuttage survival rate and use Method
Technical field
The present invention relates to a kind of auxin and application method that can improve camellia azalea cuttage survival rate.
Background technology
Camellia azalea (Camellia azalea) is also referred to as Zhang Shi Camellias, is the distinctive rare or endangered species in China, existing It is few to deposit field original seed quantity, critical species is classified as by Chinese species Red List.Camellia azalea is evergreen all the year round, and the florescence is up to More than 180 days, large flower and brilliant color, ornamental value was high, caused the highest attention on international camellia association and world gardens circle, manually Exploitation is just carried out in high gear.The main breeding practice of current camellia azalea is grafting, and operation difficulty is big, reproduction speed Slowly, it is expensive, and cuttage is to expand the preferred method of its individual amount, simple to operate, reproduction speed is fast, but cuckoo Red Hill Tea cuttage root-taking is relatively difficult, and cuttage survival rate is relatively low, and growth is slow, up for further research.
The content of the invention
It is an object of the invention to provide a kind of auxin that can improve camellia azalea cuttage survival rate and use Method.
The present invention above-mentioned technical purpose technical scheme is that:
A kind of auxin that can improve camellia azalea cuttage survival rate, it is characterised in that:The growth hormone Including methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA), the mixed volume ratio of the methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA) is 1: 4。
Preferably, the application method of a kind of auxin that can improve camellia azalea cuttage survival rate, its Step is:
(1) solution is prepared:By methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA) with 1:Growth hormone group is made in 4 ratio allotment Merge and be mixed and made into solution with water, concentration is 0.05g/L~1.00g/L.
(2) cuttings is handled:Cuttings retains top 1 to blade, and cuts off the blade first half, and the base portion of cuttings is cut with scraper Into the shape of a hoof, 5~10s of base portion is handled with auxin, inserts in seedbed of the matrix for sieving yellow soil, is poured after cuttage is good Water, covered with plastic sheeting shed and use shade net sunshade.
Preferably, the step of the application method of a kind of auxin that can improve camellia azalea cuttage survival rate Suddenly the concentration of (1) growth hormone combination is 0.50g/L.
Preferably, the step of the application method of a kind of auxin that can improve camellia azalea cuttage survival rate Suddenly (2) auxin aqueous solution processing base portion 6s.
The beneficial effects of the invention are as follows:
The invention enables the cuttage survival rate of camellia azalea cuttings to be greatly improved, and accelerates the speed of growth.
Embodiment
The present invention is described in further detail below.
A kind of auxin and application method that can improve camellia azalea cuttage survival rate, comprises the following steps:
1st, planting site selects:
The maritime monsoon climate of planting site category south subtropicses, has a moderate climate, abundant rainfall, and illumination is sufficient, average temperature of the whole year 22 degree, annual precipitation 2383mm;Red earth, soil layer is deep, soil pH value 5.75, electrical conductivity 0.55ms/cm;The content of organic matter 0.77%, soil moisture content 1.31%, N, P, K content are respectively 50,11.6,23.4mg/kg, soil fertility is poor.
2nd, cuttage seeding is chosen:
Cuttings used is lignifying branch raw then.It is red to pick up from robust growth, no disease and pests harm, the cuckoo that has no mechanical damage Camellia plant.Growth hormone species used is methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA).
3rd, growth hormone matches:
Auxin processing sets 13 levels altogether, including:Blank control (CK), that is, given birth in handling without any plant Long hormone;Methyl α-naphthyl acetate (NAA) is mixed and made into solution with water, then cuttings is handled, concentration is respectively:0.05g/L(T1)、 0.25g/L(T2)、0.5g/L(T3)、1g/L(T4);Indolebutyric acid (IBA) is mixed and made into solution with water, and then cuttings is carried out Processing, concentration are respectively:0.05g/L(T5)、0.25g/L(T6)、0.5g/L(T7)、1g/L(T8);Auxin combines (80%IBA+20%NAA) is mixed and made into solution with water, then cuttings is handled, concentration is respectively:0.05g/L(T9)、 0.25g/L(T10)、0.50g/L(T11)、1.00g/L(T12)。
4th, growth hormone application method:
Experiment is carried out in spring and summer respectively, and spring, summer was in June 15 in 15 days 2 months.Each season Handle 50 cuttings.Cutting length is basically identical, retains top 1 to blade, and cuts off the blade first half, and the base portion of cuttings is used Scraper is whittled into the shape of a hoof, and 5~10s of base portion is handled with the auxin aqueous solution, inserts in seedbed of the matrix for sieving yellow soil In, watered after cuttage is good, covered with plastic sheeting shed and use shade net sunshade, survival rate (note is checked after 45~5 days:Bud is still It then can be regarded as in green and survive).The end of the year investigates take root number, root long, takes out tip number and height of seedling.The data obtained is entered with SPSS12.5 softwares Row variance analysis and LSD sequences.
5th, result and analysis:
(1) influence of the cuttage season to each index of cuttage:
During spring cuttage, survival rate, number of averagely taking root, average root long, when tip number, then average height of seedling etc. are taken out in annual 5 investigation index average values are respectively 85.6%, 17.7,3.5cm, 1.2 times, 7.2cm;During summer cuttage, it is respectively then 88.0%th, 20.6,3.0cm, 0.8 time, 5.4cm.The average value of summer cuttage survival rate is close to spring, several average value ratios of taking root Spring cuttage is high by 16.6%, but the average root long of spring cuttage, when tip number is taken out in annual, average height of seedling then compares the summer respectively then Season is high by 19.2%, 40.7% and 32.7%.The two different cuttage seasons of Analysis of variance, spring and summer are to camellia azalea Above-mentioned 5 investigation indexs influence be not up to significant difference (p<0.05).
(2) influence of the different auxins to each index of cuttage:
Variance analysis shows, cuttage survival rate of the auxin to camellia azalea, averagely take root number and average root It is long that there is pole to significantly affect (p<0.001), taken out when annual and send significant difference (p between number and average height of seedling processing<0.05). To filter out the suitable auxin of camellia azalea, it is each to cuttage that different auxins are handled into camellia azalea The influence of index makees LSD Multiple range tests and is shown in Table 1.
1. cuttage survival rate:
Table 1 is understood, optimal with T11, T10, T8, T2 treatment effect in terms of the cuttage survival rate of camellia azalea, is reached 94.2%~98.0%, T11 cuttage survival rate highest, it is 98.0%;T7, T3, T12 also have preferable treatment effect, survive Rate is up to more than 90%;It is T6, T9, T5 again, survival rate is 82.2%~88.6%;Worst T1 in auxin processing And T4, survival rate are 71.5%~75.3%, substantially less than other any processing of auxin.Although CK survival rates 66.2% can be reached, still, cuttings base portion is most the phenomenon of the only not long root of long callus occurs, therefore number of taking root is substantially less than plant Growth hormone handles (p<0.05).This experiment single auxin in, NAA least concentration (0.05g/L) and The effect of maximum concentration (1g/L) is all significantly worse than intermediate concentration (p<0.05), middle low concentration 0.25g/L effects are best, NAA's Under high concentration (1g/L), cuttings base portion blackening, display cell comes to harm.Auxin combines (80%IBA+20% NAA) processing and minimum and maximum concentration are less than intermediate concentration, and middle and high concentration 0.5g/L rooting rate is significantly higher than minimum and most High concentration.The rooting rate of IBA processing is then raised and raised with concentration.Maximum concentration (1g/L) is significantly higher than least concentration 0.05g/ L and middle low concentration 0.25g/L (p<0.05), slightly above middle and high concentration 0.5g/L.
1 different auxins of table handle LSD Multiple range test of the camellia azalea to each cuttage Index Influence
Note:Significant difference be present between the female different expression processing of space, difference is not up to notable between having same letter expression processing (p < 0.05).
2. take root number and root long:
Several and average root long of averagely taking root reflects the robustness of cuttage plant root.From the point of view of number of averagely taking root, CK shows Write less than any auxin processing (p < 0.05), only 0.1, i.e. most of individual of CK does not all grow root system. In auxin processing, T12, T11, T4 averagely take root number at most, reach more than 30.Next to that T8, T10, T3, T7, Reach more than 20;It is T2, T6, T9 again, up to more than 10;It is finally T1 and T5, less than 10.Various plants in experiment Growth hormone, present and raised with concentration, the increased trend of quantity of taking root, under comparable sodium, auxin combination (80% IBA+20%NAA) quantity of taking root of processing is more than purebred auxin.From the point of view of average root long, CK and the life of various plants High concentration processing T12, T8, T4 of long hormone are substantially less than the processing of remaining vegetable growth hormone.At various auxins T1 in middle low concentration T6, T10, T2 and NAA low concentration of reason is most long;Next to that various auxin processing Middle and high concentration and middle low concentration.From the point of view of comprehensive averagely take root number and average root long, the processing of high concentration auxin, number of taking root Amount is more, and it is also more to consume the nutrient of cuttings, so that new root length shortens.NAA middle low concentration T2 averagely take root number for 19.5, it is flat The middle and high concentration T7 that equal root long be 4.0, IBA numbers of averagely taking root are 21.6, average root a length of 3.5, and auxin combines The middle and high concentration T11 of (80%IBA+20%NAA) averagely takes root number for 30.6, average root a length of 4.2, is all that each plant growth swashs The best concentration of rooting efficiency in element, T11 rooting efficiency is best, the rooting efficiency of auxin in 13 kinds of processing It is substantially better than single treatment.
3. take out tip number and height of seedling:
It is the index for reflecting camellia azalea aerial growth vigor averagely to take out tip number and average height of seedling.Just averagely take out From the point of view of tip number, CK and NAA high concentration T4 are minimum, only 0.2 and 0.3 time respectively;Secondly it is few be NAA middle and high concentration T3 And IBA high concentration T8;That most is T9, T10, T11 and IBA of auxin T6.Tip number is taken out as plant gives birth to The raising of long hormone concentration is in the trend of reduction after first increasing.Mixed plant growth hormone take out tip number except middle low concentration T9 and T6 in IBA processing is equal outer, and other two kinds of hormone (p are significantly more than under comparable sodium<0.05).Come from average height of seedling See, all in floor level, wherein CK is even more only 0.5cm average height of seedling by CK and T3, T4, T8, this and CK cuttings base portion A large amount of Callus formations and dross, not long root, most of nutrition have been consumed relevant.The processing of average height of seedling highest includes planting The T6 of T10, T11 and T12 and IBA in thing growth hormone.Average height of seedling is also that carrying with auxin concentration is presented The trend that Gao Chengxian is reduced after increasing.In addition to low concentration, the average height of seedling of auxin is higher than other comparable sodiums Single treatment.It is comprehensive averagely take out tip number and average height of seedling from the point of view of, aerial growth vigor most preferably T10, T11 and T6, T10, T11 and T6 average height of seedling are respectively 2.0,1.7,1.4, and it is respectively 10.6,9.6,8.5 averagely to take out and send number.
6th, conclusion:
(1) simultaneously there was no significant difference for spring and summer cuttage camellia azalea.Auxin processing is to cuckoo Red Hill The survival rate of tea, number of averagely taking root, average root long, averagely take out tip number and average height of seedling makes a significant impact (p<0.05).
(2) from the point of view of comprehensive every cuttage index, it is easy to see that in 13 kinds of processing, effect most preferably 0.5g/L plant life Long hormone combinations (80%IBA+20%NAA), surviving during 0.5~1.0 year camellia azalea cuttings cuttage 45~50 days can be made Being averaged when rate reaches 98.0%, 1 year, which takes root for counting, reaches 30.6, and average root length reaches 4.2cm, averagely takes out tip number and reaches 1.7 times, average height of seedling reaches 9.6cm, has pole to be greatly improved than control (CK).Show under the plant growth hormone concentration The survival rate of camellia azalea cuttage, rooting efficiency and overground part growth vigor are all ideal.
(3) most preferably mixed from the point of view of the cutting experiment of camellia azalea under comprehensive various auxin comparable sodiums Hormone combinations (80%IBA+20%NAA) are closed, cutting experiment is used alone better than NAA and IBA.
(4) each auxin to the cutting experiment of camellia azalea substantially with the concentration rise of auxin and The trend reduced afterwards in first improving.NAA optium concentration is 0.25g/L, and IBA optium concentration is 0.5g/L, hormotone group The optium concentration for closing (80%IBA+20%NAA) is also 0.5g/L.
This specific embodiment is only explanation of the invention, and it is not limitation of the present invention, people in the art Member can make the modification of no creative contribution to the present embodiment as needed after this specification is read, but as long as at this All protected in the right of invention by Patent Law.

Claims (4)

  1. A kind of 1. auxin that can improve camellia azalea cuttage survival rate, it is characterised in that:The growth hormone bag Include methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA), the mixed volume ratio of the methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA) is 1:4.
  2. 2. a kind of application method for the auxin that can improve camellia azalea cuttage survival rate, its step are:
    (1) solution is prepared:By methyl α-naphthyl acetate (NAA) and indolebutyric acid (IBA) with 1:Growth hormone combination is made simultaneously in 4 ratio allotment Solution is mixed and made into water, concentration is 0.05g/L~1.00g/L.
    (2) cuttings is handled:Cuttings retains top 1 to blade, and cuts off the blade first half, and the base portion of cuttings is whittled into horse with scraper It is ungulate, 5~10s of base portion is handled with the auxin aqueous solution, is inserted in seedbed of the matrix for sieving yellow soil, after cuttage is good Watering, covered with plastic sheeting shed and use shade net sunshade.
  3. A kind of 3. user of auxin that can improve camellia azalea cuttage survival rate according to claim 2 Method, it is characterised in that:The concentration of step (1) the growth hormone combination is 0.50g/L.
  4. A kind of 4. user of auxin that can improve camellia azalea cuttage survival rate according to claim 2 Method, it is characterised in that:Step (2) the growth hormone aqueous solution processing base portion 6s.
CN201710956822.0A 2017-10-16 2017-10-16 A kind of auxin and application method that can improve camellia azalea cuttage survival rate Pending CN107568227A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834701A (en) * 2018-04-03 2018-11-20 金华市泽雨园艺技术有限公司 The breeding and seedling method of camellia
CN114982491A (en) * 2022-07-08 2022-09-02 湖南省植物园 Cutting propagation method of monomer camellia japonica
CN117581874A (en) * 2024-01-19 2024-02-23 北京林业大学 Composite plant growth regulator for cutting azalea and cutting method of azalea
CN117581871A (en) * 2024-01-19 2024-02-23 北京林业大学 Composite plant growth regulator for tea plum cutting and tea plum cutting method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104886136A (en) * 2015-05-27 2015-09-09 广西苷亮健生物科技有限公司 Mixed rooting agent suitable for various plants
CN106900329A (en) * 2017-03-03 2017-06-30 广西壮族自治区农业科学院花卉研究所 The method of camellia azalea cuttage and quick-propagation

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104886136A (en) * 2015-05-27 2015-09-09 广西苷亮健生物科技有限公司 Mixed rooting agent suitable for various plants
CN106900329A (en) * 2017-03-03 2017-06-30 广西壮族自治区农业科学院花卉研究所 The method of camellia azalea cuttage and quick-propagation

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108834701A (en) * 2018-04-03 2018-11-20 金华市泽雨园艺技术有限公司 The breeding and seedling method of camellia
CN114982491A (en) * 2022-07-08 2022-09-02 湖南省植物园 Cutting propagation method of monomer camellia japonica
CN117581874A (en) * 2024-01-19 2024-02-23 北京林业大学 Composite plant growth regulator for cutting azalea and cutting method of azalea
CN117581871A (en) * 2024-01-19 2024-02-23 北京林业大学 Composite plant growth regulator for tea plum cutting and tea plum cutting method
CN117581874B (en) * 2024-01-19 2024-03-26 北京林业大学 Composite plant growth regulator for cutting azalea and cutting method of azalea
CN117581871B (en) * 2024-01-19 2024-05-17 北京林业大学 Composite plant growth regulator for tea plum cutting and tea plum cutting method

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