CN117581874B - Composite plant growth regulator for cutting azalea and cutting method of azalea - Google Patents
Composite plant growth regulator for cutting azalea and cutting method of azalea Download PDFInfo
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- CN117581874B CN117581874B CN202410081518.6A CN202410081518A CN117581874B CN 117581874 B CN117581874 B CN 117581874B CN 202410081518 A CN202410081518 A CN 202410081518A CN 117581874 B CN117581874 B CN 117581874B
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- azalea
- growth regulator
- plant growth
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- 238000005520 cutting process Methods 0.000 title claims abstract description 70
- 241000208422 Rhododendron Species 0.000 title claims abstract description 58
- 239000005648 plant growth regulator Substances 0.000 title claims abstract description 26
- 239000002131 composite material Substances 0.000 title claims abstract description 18
- 238000000034 method Methods 0.000 title claims abstract description 14
- JTEDVYBZBROSJT-UHFFFAOYSA-N indole-3-butyric acid Chemical compound C1=CC=C2C(CCCC(=O)O)=CNC2=C1 JTEDVYBZBROSJT-UHFFFAOYSA-N 0.000 claims abstract description 42
- VAMXMNNIEUEQDV-UHFFFAOYSA-N methyl anthranilate Chemical compound COC(=O)C1=CC=CC=C1N VAMXMNNIEUEQDV-UHFFFAOYSA-N 0.000 claims abstract description 36
- 229940102398 methyl anthranilate Drugs 0.000 claims abstract description 18
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 4
- 239000007864 aqueous solution Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims 2
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims 2
- 239000006013 carbendazim Substances 0.000 claims 2
- 230000002195 synergetic effect Effects 0.000 abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 25
- 241000196324 Embryophyta Species 0.000 description 16
- 238000004090 dissolution Methods 0.000 description 10
- 230000012010 growth Effects 0.000 description 8
- 239000003630 growth substance Substances 0.000 description 8
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 6
- 239000004480 active ingredient Substances 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000011159 matrix material Substances 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 4
- 239000012752 auxiliary agent Substances 0.000 description 4
- 239000011230 binding agent Substances 0.000 description 4
- 230000001737 promoting effect Effects 0.000 description 4
- 238000010008 shearing Methods 0.000 description 4
- 238000002791 soaking Methods 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000002985 plastic film Substances 0.000 description 3
- 229920006255 plastic film Polymers 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- 229930192334 Auxin Natural products 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- XLOMVQKBTHCTTD-UHFFFAOYSA-N Zinc monoxide Chemical compound [Zn]=O XLOMVQKBTHCTTD-UHFFFAOYSA-N 0.000 description 2
- 239000013011 aqueous formulation Substances 0.000 description 2
- 239000002363 auxin Substances 0.000 description 2
- 239000004927 clay Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 235000019441 ethanol Nutrition 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 239000003617 indole-3-acetic acid Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000012286 potassium permanganate Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000021749 root development Effects 0.000 description 2
- -1 1.2g is weighed Substances 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 239000005995 Aluminium silicate Substances 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 235000012211 aluminium silicate Nutrition 0.000 description 1
- 229960000892 attapulgite Drugs 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 229910052570 clay Inorganic materials 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000010440 gypsum Substances 0.000 description 1
- 229910052602 gypsum Inorganic materials 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010445 mica Substances 0.000 description 1
- 229910052618 mica group Inorganic materials 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- 229910052625 palygorskite Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000454 talc Substances 0.000 description 1
- 229910052623 talc Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000004408 titanium dioxide Substances 0.000 description 1
- 239000011787 zinc oxide Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a composite plant growth regulator for azalea cutting and a cutting method of azalea. The compound plant growth regulator for azalea cuttage comprises effective components, wherein the effective components comprise methyl anthranilate and indolebutyric acid, and the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.08-0.9: 20-25. The invention effectively shortens the rooting time of the rhododendrons of spring rhododendrons, xia Juan and other varieties by utilizing the synergistic effect of the two components, and can effectively improve the rooting rate and increase the rooting number.
Description
Technical Field
The application relates to the field of plant growth regulators, in particular to a composite plant growth regulator for azalea cutting and a cutting method of azalea.
Background
The azalea is one of ten flowers in China, has the American name of 'queen in flowers', is widely applied to landscaping and courtyard ornamental in China, and is popular among people. The variety of azalea in China is relatively large. Wherein, spring rhododendron refers to a rhododendron variety which blooms first and then germinates, and blooms early in four months, late in late ten days and early in five months, and is named spring rhododendron because of blooming in spring, and is often applied to ground cover color blocks or landscaping. Xia Juan is characterized by that it can make long branches and leaves first in spring, and can make flowers at the beginning of 5-6 months and can last for 7-8 months, so that it is called rhododendron. However, although azalea is a large variety, the protection work for the plant of the genus is still insufficient, so it is urgent to accelerate the breeding of varieties with high economic value such as azalea and Xia Juan. At present, the cutting propagation has the advantages of low propagation cost and capability of better maintaining the excellent characteristics of the female parent of azalea. However, the current rhododendron cutting technology is not mature enough, and the problems of low rooting rate, long rooting period and the like still exist. The growth regulator has important promoting effect on plant cutting rooting. At present, the research of plant growth regulators is not few, but the components and the types of many regulators are single, the effect on the spring rhododendrons and Xia Juan which are difficult to survive in cutting rooting is not obvious, and the research of the growth regulators specially aiming at the spring rhododendrons and Xia Juan cutting rooting has little breakthrough. New technical means are needed to solve the above problems.
For this purpose, the present invention is proposed.
Disclosure of Invention
The invention mainly aims to provide a composite plant growth regulator for azalea cutting and a cutting method of azalea, wherein the regulator utilizes the synergistic effect of two components to effectively shorten the rooting time of azalea cutting, and greatly increase the rooting quantity and quality.
In order to achieve the above object, the present invention provides the following technical solutions.
The invention provides a compound plant growth regulator for azalea cutting, which comprises effective components, wherein the effective components comprise methyl anthranilate and indolebutyric acid, and the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.08-0.9: 20-25.
Therefore, the invention takes the methyl anthranilate and the indolebutyric acid as the effective components, and can achieve the following effects: the addition of the methyl anthranilate can effectively promote the synthesis of indoleacetic acid in plants, and the indoleacetic acid and the added indolebutyric acid act together, so that the content of auxin in cuttings can be rapidly increased, the formation of adventitious roots is promoted, the rooting time is shortened, and the rooting quantity and the rooting length are effectively increased. In a word, the invention utilizes the synergy of the methyl anthranilate and the indolebutyric acid to play a 1+1>2 effect on azalea cuttage.
The composite plant growth regulator for azalea cuttage has outstanding rooting promoting effect on azalea, especially on azalea and Xia Juan which are difficult to survive in cuttage rooting.
The composite plant growth regulator for azalea cuttage can only contain active ingredients, and can also form a stable formulation with an auxiliary agent.
Further, the adhesive further comprises an auxiliary agent, wherein the auxiliary agent comprises at least one of a binder, a filler, a solvent and a stabilizer.
The auxiliary agents are favorable for preparing the active ingredients into a preparation form with good stability and favorable absorption by plants.
The binder may be one or more of clay, bentonite, montmorillonite, attapulgite and kaolin, gypsum, cellulose, titanium dioxide, zinc oxide, starch, dextrin, sugar (e.g. lactose, sucrose), silica, talc, mica, diatomaceous earth, urea, etc. These binders help to stably bind the active ingredient to the root/basal surface of the plant, and to prolong the contact time, thereby improving the efficacy.
The solvent can be a component which can uniformly disperse the active ingredient and has little or no damage to the plant rhizome. For example, the active ingredient is usually dissolved in a small amount of an organic solvent such as ethanol, and then diluted with water to a constant volume.
Further, the binder includes clay.
Further, the solvent includes water. In some embodiments, the solvent is primarily water, possibly with a very small amount of organic solvents such as ethanol.
Further, the composite plant growth regulator is powder or water aqua.
Further, the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.45-0.75: 20-25. The synergistic effect of the two components is more remarkable at this time.
Further, the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.45-0.75: 20-23.
Further, the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.45-0.75: 23-25.
Further, in the aqueous solution, the concentration of methyl anthranilate is 0.08-0.9%, and the concentration of indolebutyric acid is 20-25%. "%" means the percentage of the corresponding components in the total mass of the solution.
Further, in the aqueous solution, the concentration of methyl anthranilate is 0.45-75%, and the concentration of indolebutyric acid is 20-25%.
Furthermore, the composite plant growth regulator can be used for cutting various plants, and especially has remarkable rooting promoting effect when being used for cutting azalea. Rhododendron can be spring Rhododendron, xia Juan, etc.
In addition, the composite plant growth regulator can also be applied to direct planting, transplanting and the like of plants.
The second aspect of the invention provides a cutting method of azalea, comprising:
soaking the cutting of the azalea in the solution containing the composite plant growth regulator of the first aspect for 2-3 hours, and then taking out the cutting of the azalea and inserting the cutting into a matrix for culturing.
Soaking for 2-3 hours can enable tissues of rhododendron cutting slips to fully absorb the growth agent.
The solution containing the composite plant growth regulator is referred to as a solution system. If the composite plant growth regulator is powder, water and other solvents may be added to dissolve or disperse. In the case of aqueous formulations, the aqueous formulations may be used as they are, with or without dilution.
The cuttings are usually obtained by the following means: taking 1-2 internodes of the current annual semi-lignified branch of the rhododendron, horizontally shearing an upper opening and obliquely shearing a lower opening to serve as a cutting branch.
Further, the environment during the culture is: the humidity is above 80%, the daytime temperature is 23-28 ℃, and the night temperature is more than or equal to 18 ℃.
Further, the length of the cutting slips is 5-7 cm, and 1-2 leaves are reserved at the tops of the cutting slips.
Further, the cutting retains at least 1 axillary bud.
Further, the matrix may include loess and silt.
Further, the substrate can be infiltrated and sterilized with 0.3% potassium permanganate before cutting.
Further, the plastic film can be covered at the initial stage for heat preservation and moisture preservation during the culture, and the plastic film can be removed at the later stage. For example, when 60% of the cuttings grow adventitious roots during batch cutting, the plastic film can be removed.
Further, leaves reserved at the top of the cutting slips before cutting can be cut off by 1/3-1/2 so as to improve the growth speed of the paper strips.
Further, the shading degree can be controlled to be 60% -70% in the culture process.
In conclusion, compared with the prior art, the invention achieves the following technical effects:
(1) The plant growth regulator taking the methyl anthranilate and the indolebutyric acid as the effective components can effectively promote the synthesis of indolebutyric acid in plants, and the synthesis of indolebutyric acid in plants can be combined with the added indolebutyric acid, so that the content of auxin in rhododendron cutting slips can be rapidly improved, the formation of adventitious roots is promoted, the rooting time is shortened to be at least 30 days from 60 days, and the rooting quantity and the rooting length are effectively increased.
(2) The culture condition of azalea during cutting and the appearance characteristics of cutting are optimized, so that the rooting efficiency is further improved.
The foregoing description is only an overview of the technical solutions of the present application, and may be implemented according to the content of the specification in order to make the technical means of the present application more clearly understood, and in order to make the above-mentioned and other objects, features and advantages of the present application more clearly understood, the following detailed description of the present application will be given.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the application. Also, like reference numerals are used to designate like parts throughout the accompanying drawings. In the drawings:
FIG. 1 shows the growth of rhododendron;
FIG. 2 shows the growth of spring azalea;
FIG. 3 is a photograph of a cutting seedbed of a rhododendron;
fig. 4 is a photograph of a cutting seedbed of spring azalea.
Detailed Description
Embodiments of the technical solutions of the present application will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical solutions of the present application, and thus are only examples, and are not intended to limit the scope of protection of the present application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description and claims of the present application and in the description of the figures above are intended to cover non-exclusive inclusions.
In the description of the embodiments of the present application, the meaning of "plurality" is two or more unless explicitly defined otherwise.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the present application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
Unless otherwise specified in the examples and control groups below, "ANT" refers to methyl anthranilate and "IBA" refers to indolebutyric acid.
Example 1
Firstly, weighing raw materials with corresponding mass according to concentration: 0.08% of ANT,23% of IBA, absolute ethyl alcohol and a proper amount of water.
The ANT is dissolved in a small amount of absolute ethyl alcohol, and the amount of absolute ethyl alcohol is determined by the complete dissolution of the ANT, but is not excessive. Obtaining an ANT solution after dissolution; the IBA powder is dissolved in a proper amount of absolute ethyl alcohol, and the absolute ethyl alcohol is also used for completely dissolving IBA, but the IBA powder is not excessive, and the stirring time can be increased instead of increasing the absolute ethyl alcohol. And (5) obtaining the IBA solution after the dissolution is completed.
Mixing the ANT solution and the IBA solution together, and adding water to a volume of 1L to obtain the required growth regulator.
Example 2
Firstly, weighing raw materials with corresponding mass according to concentration: 0.45% of ANT,23% of IBA, absolute ethyl alcohol and a proper amount of water.
The ANT is dissolved in a small amount of absolute ethyl alcohol, and the amount of absolute ethyl alcohol is determined by the complete dissolution of the ANT, but is not excessive. Obtaining an ANT solution after dissolution; the IBA powder is dissolved in a proper amount of absolute ethyl alcohol, and the absolute ethyl alcohol is also used for completely dissolving IBA, but the IBA powder is not excessive, and the stirring time can be increased instead of increasing the absolute ethyl alcohol. And (5) obtaining the IBA solution after the dissolution is completed.
Mixing the ANT solution and the IBA solution together, and adding water to a volume of 1L to obtain the required growth regulator.
Example 3
Firstly, weighing raw materials with corresponding mass according to concentration: 0.75% of ANT,23% of IBA, absolute ethyl alcohol and a proper amount of water.
The ANT is dissolved in a small amount of absolute ethyl alcohol, and the amount of absolute ethyl alcohol is determined by the complete dissolution of the ANT, but is not excessive. Obtaining an ANT solution after dissolution; the IBA powder is dissolved in a proper amount of absolute ethyl alcohol, and the absolute ethyl alcohol is also used for completely dissolving IBA, but the IBA powder is not excessive, and the stirring time can be increased instead of increasing the absolute ethyl alcohol. And (5) obtaining the IBA solution after the dissolution is completed.
Mixing the ANT solution and the IBA solution together, and adding water to a volume of 1L to obtain the required growth regulator.
Control group 1
And (5) clean water.
Control group 2
The Guoguang rooting powder is diluted by 800 times of liquid, 1.2g is weighed, and water is added for dilution to 1L.
Control group 3
And (3) treating common rapid rooting powder (msh-sgf plant rooting powder for diffuse life).
Control group 4
Indolebutyric acid: weighing indolebutyric acid, putting into a beaker, adding absolute ethyl alcohol for dissolution, and diluting to 1L with a large amount of water, wherein the final concentration is 23%.
The above examples and control groups were used for cutting Xia Juan and azalea, as follows.
The test steps are as follows:
1. cutting matrix: the cutting bed is filled with a matrix mixed by loess and river sand, and is wetted, sterilized and disinfected by 0.3% potassium permanganate in the day before cutting.
2. Selecting insertion snapping: taking 1-2 internodes of current annual semi-lignified branches of spring rhododendrons and Xia Juan, and horizontally shearing at the upper opening and obliquely shearing at the lower opening to serve as cutting branches; the length of each cutting branch is 5-7 cm, 1-2 leaves are reserved at the top of each cutting branch (1/3-1/2 of the leaves reserved at the top are cut off), and at least 1 axillary bud is reserved.
3. Soaking a growth agent: and selecting the products of the previous examples and the control group, and soaking the cutting slips obtained in the 2 nd point in a growth agent for 2-3 hours. 120 cuttings are treated separately for each example and control group as a group.
4. Culturing:
cutting the treated cuttings into seedbeds uniformly and orderly, marking each treatment, coating the seedbeds, controlling the daytime temperature in the film at 23-28 ℃ and the humidity at 80-85%, and controlling the nighttime temperature at not less than 18 ℃. Observing every other day from the next day of cutting, and supplementing matrix water in time when the humidity is too low. In the process of growing the cutting branches, the time length required by rooting of different treatments is compared. After 50 days of culture, determining physiological data such as rooting rate, average root length, root cluster diameter, plant height, branch number and the like of different treatments; and then according to a random sampling method, randomly taking 3 plants as experimental samples by an example group and a control group, and comparing the number of root systems and the development conditions of different treatments. Fig. 3 and 4 show the cutting beds of example 1 for Xia Juan and spring azalea, respectively.
5. Results:
the data is counted as rooting conditions of 50 days of cuttage, the rooting conditions of Xia Juan are shown in figure 1, and the rooting conditions of spring rhododendrons are shown in figure 2.
The root mass length, diameter, plant height, branch number and rooting rate of Xia Juan are shown in table 1. The root mass length, diameter, plant height, branch number and rooting rate of spring rhododendron are shown in Table 2.
Wherein rooting rate= (number of rooting branches/total number of cutting branches) ×100%
Table 1 comparison of growth conditions of rhododendron
TABLE 2 rooting Condition comparison of spring Azalea
The results show that the examples have obvious advantages over the control group, both in terms of rooting rate and rooting quantity and root development state. The effects of the embodiment 2 and the embodiment 3 are particularly remarkable, the indexes such as root cluster diameter and plant height are remarkably improved, and the growth and development of the cuttage branches are promoted. Compared with other brands of growth regulators, the growth regulator can effectively shorten the rooting time, shorten the time for generating the callus and shorten the time from 60 days to 30 days. Compared with the commercial growth regulator, the rooting rate and the rooting quantity of the cutting branches are improved, the root development is promoted, the rooting time is effectively shortened, and the rooting promoting effect is good for spring rhododendron and Xia Juan cutting.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the embodiments, and are intended to be included within the scope of the claims and description. In particular, the technical features mentioned in the respective embodiments may be combined in any manner as long as there is no structural conflict. The present application is not limited to the specific embodiments disclosed herein, but encompasses all technical solutions falling within the scope of the claims.
Claims (10)
1. The compound plant growth regulator for azalea cutting is characterized by comprising effective components, wherein the effective components comprise methyl anthranilate and indolebutyric acid, and the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.08-0.9: 20-25.
2. The composite plant growth regulator according to claim 1, wherein the composite plant growth regulator is a powder or a water aqua.
3. The compound plant growth regulator according to claim 1, wherein the mass ratio of the methyl anthranilate to the indolebutyric acid is 0.45 to 0.75: 20-25.
4. The compound plant growth regulator according to claim 2, wherein the concentration of methyl anthranilate in the aqueous solution is 0.08 to 0.9%, and the concentration of indolebutyric acid is 20 to 25%.
5. The composite plant growth regulator according to claim 1, wherein the composite plant growth regulator is used for cutting of azalea.
6. A cutting method of azalea, comprising:
immersing the cutting of azalea in the solution containing the composite plant growth regulator according to any one of claims 1-5 for 2-3 hours, and then taking out and inserting the cutting into a substrate for culturing.
7. The cutting method according to claim 6, wherein the environment at the time of cultivation is: the humidity is above 80%, the daytime temperature is 23-28 ℃, and the night temperature is more than or equal to 18 ℃.
8. The cutting method according to claim 6, wherein the length of the cutting slips is 5-7 cm, and 1-2 leaves are reserved at the tops of the cutting slips;
and/or, the cutting leaves retain at least 1 axillary bud.
9. The cutting method according to claim 6, wherein the substrate is sterilized with carbendazim prior to cutting.
10. The cutting method according to claim 6, wherein the substrate is sterilized with carbendazim every 1 to 2 weeks during the cultivation.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1114129A (en) * | 1994-06-16 | 1996-01-03 | 王华芳 | Process for breeding and culturing azalea |
| CN107568227A (en) * | 2017-10-16 | 2018-01-12 | 佛山市林业科学研究所 | A kind of auxin and application method that can improve camellia azalea cuttage survival rate |
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| BR112014002210A2 (en) * | 2011-08-02 | 2017-03-07 | Basf Se | aqueous liquid composition, method for preparing the composition, method for combating harmful insects and / or phytopathogenic fungi and method for controlling unwanted vegetation |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1114129A (en) * | 1994-06-16 | 1996-01-03 | 王华芳 | Process for breeding and culturing azalea |
| CN107568227A (en) * | 2017-10-16 | 2018-01-12 | 佛山市林业科学研究所 | A kind of auxin and application method that can improve camellia azalea cuttage survival rate |
Non-Patent Citations (2)
| Title |
|---|
| Hammerschlag F.Factors Influencing in Vitro Multiplication and Rooting of the Plum Rootstock Myrobalan (Prunus cerasifera Ehrh.) 1.Journal of the American Society for Horticultural Science.第107卷(第1期),44-47. * |
| 吲哚丁酸浓度及穗条形态对杜鹃扦插的影响;吴虎南等;南京农专学报;19981231;第14卷(第1期);15-18 * |
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