CN1114129A - Process for breeding and culturing azalea - Google Patents

Process for breeding and culturing azalea Download PDF

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Publication number
CN1114129A
CN1114129A CN 94106373 CN94106373A CN1114129A CN 1114129 A CN1114129 A CN 1114129A CN 94106373 CN94106373 CN 94106373 CN 94106373 A CN94106373 A CN 94106373A CN 1114129 A CN1114129 A CN 1114129A
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breeding
rooting promoter
azalea
catechol
water
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CN 94106373
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Chinese (zh)
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王华芳
宋久旺
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Abstract

The breeding and culturing method for azalea, especially for Belgium azalea features use of efficient root promoter, nutritive solution and culture medium to accelerate root growing. In its industrial production, the input and output ratio reaches 1:10.

Description

The process for breeding and culturing of a kind of azalea
The present invention relates to the process for breeding and culturing of flower cultivation method, particularly azalea.
Azalea is world-renowned ornamental flower, also is one of three well-known big famous flowers of China.The azalea pattern is delicate and charming, and agglomerating cluster is driveed very ardent in the end of the branch, very easily excites people's affection and inspiration, is subjected to generally liking of people.
Belgium four seasons cuckoo is the new varieties of introducing from Belgium recent years.Its shape, look, appearance, even etc. all tool is good.Welcome by domestic and international consumer, market prospects are good.But backward in technique aspect breeding and culturing can not be in the wide geographic area common cultivated in the whole nation, to entering the problem that family and senior place all exist breeding and culture technique not to pass a test.
At present, the propagation method of azalea have that seed is broadcast, method such as cuttage, grafting and press strip.The major defect of these methods is:
1, the kind that has, particularly flowers treasure (comprising cuckoo), polyphyll is serious, and is colored and unreal so that the stamen serious degradation can not produce flower pesticide, can't use seminal propagation.Both made on the other hand and adopted artificial pollination method to obtain seed, also because sexual propagation causes proterties division, kind serious degradation.
2, vegetative propagation can keep varietal character, is difficult for taking root and survives but the problem that exists is (1) cuttage, does not also take root in 2 months as Belgium four seasons cuckoo, and breeding cycle is long, reproduction speed is slow, and shortened vegetative period, influences its ornamental value.(2) grafting scion and stock exist hereditary difference thereby affinity not, and survival rate is low.(3) planting of layer can not form commercialization production.
3, have the people once to propose method for tissue culture, and done many experiments, but because reproduction coefficient is low and the difficulty of taking root can not be successfully used to commercialization production, particularly the tissue culture of Belgium four seasons cuckoo yet there are no report so far.
The cultivation of azalea still is earth culture at present.The major defect of this method is: 1) the soil acidity or alkalinity difference has limited the azalea cultivation area, azalea happiness acid ground, and the north mainly is basic soil, is not suitable for planting cuckoo.
2) soil constituent complexity, uncontrollable factor is many, is difficult to the science of accomplishing and grows flower.
3) soil dust fall contaminated environment, sticking heavy inconvenience carrying has limited the strange land transportation and grows flower on top grade and skyscraper and admire the beauty of flowers.
4) relate to the territory problem, be not easy to international exchange and commerce and trade.
The objective of the invention is to overcome above-mentioned defective, provide a kind of commercialization to produce the method for azalea.
Method of the present invention comprises breeding and the cultivation of azalea, and the used rooting promoter of wherein said breeding contains indolebutyric acid, catechol and polyethylene pyrrolinone (PVP).Also can contain talcum powder in addition.Available ethanol is made solvent.The proportioning of described each composition can change arbitrarily as the case may be.A kind of optimization formula is that every 100ml rooting promoter contains 0.05-0.15g indolebutyric acid, 0.025-0.075g catechol, 0.05-0.15 polyethylene pyrrolinone, 10-80ml ethanol, and all the other are water.Another kind of optimization formula is that every 100g talcum powder is joined 0.5-1.5g indolebutyric acid, 0.25-0.75g catechol and 0.5-1.5g polyethylene pyrrolinone, and this rooting promoter can be earlier with being dried into powdery again after the ethanol water dissolving evenly.
A kind of preferred version of described breeding comprises the steps:
1) sets up the aseptic culture system;
2) induce the explant Growth and Differentiation;
3) promote adventitious buds proliferation;
4) strong sprout;
5) handle with rooting promoter;
6) root induction;
7) seedling management.
The medium of described aseptic culture system preferably contains nitrate of lime, ammonium nitrate, potassium nitrate, sodium hydrogen phosphate, potassium dihydrogen phosphate, magnesium sulfate, ferrous sulfate, EDTANa 2, boric acid, manganese sulphate, sodium molybdate, zinc sulphate, copper sulphate, cobalt oxide, potassium iodide, inositol, glycine, thiamine hydrochloride, sucrose, zeatin, heteroauxin, agar and water.Each composition preferred content is that every liter of culture fluid contains 4 water nitrate of lime 413-1241mg, ammonium nitrate 60-180mg, potassium nitrate 253-759mg, sodium hydrogen phosphate 300-500mg, potassium dihydrogen phosphate 68-204mg, 7 water magnesium sulfate 246-740mg, ferrous sulfate 13.9-41.7mg, EDTANa 218.6-55.8mg, boric acid 3.1-9.3mg, 4 water manganese sulphate 11.1-33.5mg, 2 water sodium molybdate 0.12-0.38mg, 7 water sulphur zinc 4.3-12.9mg, 5 brochanite 0.012-0.038mg, 6 water cobalt chloride 0.012-0.038mg, potassium iodide 0.41-1.25mg, inositol 50-150mg, glycine 1-3mg, thiamine hydrochloride (VB 1) 0.2-0.6mg, sucrose 5000-15000mg, zeatin: (opening beginning 0.5-2mg, subculture 0.2-1mg), heteroauxin: (opening beginning 10-100 μ g, subculture 0-100 μ g), agar 3000-9000mg, all the other are deionized water or distilled water.
The another kind of scheme of described breeding comprises produces the green stem cutting bar of semi-lignified, system fringe, rooting promoter processing, root induction and seedling management.Wherein rooting promoter can be used above-mentioned rooting promoter.
Cultivation in the inventive method is preferably soilless culture, uses soilless substrate and nutrient solution, comprises seedling is moved into soilless substrate, pouring nutrient solution and management.Nutrient solution used Ca (the NO that preferably contains 3) 2, NH 4NO 3, KNO 3, Na 2HPO 4, polyethylene pyrrolinone (PVP), KH 2PO 4, MgSO 4, FeSO 4, EDTA, HBO 3, MnSO 4, ZnSO 4, NaMoO 4, CuSO 4, CoCl 2, CH 3COOH, CH 3COONa and water.Each composition preferred content is that every premium on currency is furnished with 413-1241mg Ca (NO 3) 24H 2O, 60-180mg NH 4NO 3, 253-759mgKNO 3, 320-540mgNa 2HPO 4, 50-150mg (PVP), 68-204mgKH 2PO 4, 246-740mgMgSO 47H 2O, 13.9-41.7mgFeSO 4, 18.6-55.8mgEDTANa 2, 3.1-9.3mgHBO 3, 11.1-33.5mgMnSO 44H 2O, 4.3-12.9mgZnSO 47H 2O, 0.12-0.38mgNa 2MoO 42H 2O, 0.012-0.038mgCuSO 45H 2O, 0.012-0.038mg CoCl 26H 2O, 3000-9000mgCH 3COOH and 12247-36741mgCH 3COONa.
The major advantage of method of the present invention is as follows:
1, provides the particularly feasible and obvious results method for tissue culture of Belgium four seasons cuckoo of a kind of azalea.The propagation mode is sprouted for the base portion induced bundle, can not cause culture materials aging.The rate of increase calculates by present computational methods, and propagation reached 3-8 doubly in per 45 days, and year propagation reaches 6561-1.6 * 10 7Doubly, promptly output reaches 6561-16000000 young plant/material year.Solve the problem of the rooting of vitro seedling difficulty of tissue culture technique, can form batch production production.
2, provide a kind of short root technology.What take root cycle from the past shortens to 20 days more than 60 days, raise the efficiency 3 times.The amount of taking root improves 5-10 when not handling doubly.The seedling growth phase increases about 40 days, has improved flowers and trees quality and commodity value.
3, provide a kind of cultivation technique without soil.The various nutrients that the fair supply azalea is required, plant strain growth stalwartness, leaf look, flower appearance grace.Improve nutrientuse efficiency, reduced the fixing and loss of nutrient in soil.Promote flower bud differentiation, after the pouring nutrient solution, promptly have a large amount of buds to form in 3-4 week on the top of branch, in view of the above can the Artificial Control florescence.Wide accommodation is not limited by time, place and soil condition, and various places all can be cultivated.Safety and sanitation, no dust fall, free from extraneous odour are fit to various families, hotel, restaurant and all graceful place breed and view and admire.Potted flower is short and small, light, and the restriction of no territory is fit to transportation both at home and abroad and exchanges.Commodity value improves greatly.Output/input=10/1.
Further describe the present invention with embodiment below.
Embodiment 1
At first with the organization material surface sterilization of Belgium four seasons cuckoo, the thimerosal prescription is for adding 10% 95% ethanol, 1% Tween-20 in the aqueous solution that contains 0.1%HgCl, and disinfecting time is 3 minutes.Explant is stem apex and axillalry bud.Medium is that every liter of medium contains 827mgCa (NO 3) 24H 2O, 120mgNH4NO 3, 506mgKNO 3, 380mgNa 2HPO 4, 136mgKH 2PO 4, 493mgMgSO 47H 2O, 27.8mgFeSO 4, 37.2EDTANa 2, 6.2mgHBO 3, 22.3mgMnSO 44H 2O, 0.25mgNa 2MoO 42H 2O, 8.6mgZnSO 47H 2O, 0.025mgCuSO 4.5H 2O, 0.025mgCoCl 2.6H 2O, 0.83mgKI, 100mg inositol, 2mg glycine, 0.4mg thiamine hydrochloride (VB 1), 1000mg sucrose, (open the beginning: the 1mg zeatin, subculture: the 0.5mg zeatin), (open the beginning: 50 μ g heteroauxins, subculture: 0.5 μ g heteroauxin), 6000mg agar all the other for deionized water or distilled water.Handle with rooting promoter after turning out test-tube plantlet.A kind of aqua prescription of used rooting promoter is: indolebutyric acid 0.1g, catechol 0.05g, 0.1gPVP, 50% ethanol water 100ml, compound method is for to pour ethanol water in the mixture of indolebutyric acid and catechol into.A kind of wp formula of used rooting promoter is: indolebutyric acid 1g, catechol 0.5g, 1gPVP, 50% ethanol water 25ml, talcum powder 100g, compound method is for pouring ethanol water in indolebutyric acid and the catechol into earlier, talcum powder is poured in the ethanolic solution, the limit bevelling stirs, 76 ℃ of oven dry down, stir loose talcum pulvis until powdered then with glass bar.After handling with rooting promoter with the seedling cuttage in the matrix of perlite or 2/3 perlite and 1/3 peat, keep ambient humidity, light and temperature 75%, temperature 15-25 ℃.Described cultivation matrix can also be with following any: (1) 3/5 perlite+2/5 peat; (2) sphagna; (3) pine needle of Fu Laning; (4) the coniferous tree sawdust of Fu Laning.Add nutrient solution then, and per 1 to several weeks water one time of nutrition liquid, can water when matrix is done.Nutrient solution used be furnished with 827mgCa (NO for every premium on currency 3) 24H 2O, 120mgNH 4NO 3, 506mgKNO 3, 380mgNa 2HPO 4, 100mgPVP, 136mgKH 2PO 4, 493mgMgSO 47H 2O, 27.8mgFeSO 4, 37.2EDTANa 2, 6.2mgHBO 3, 22.3mg MnSO 44H 2O, 8.6mgZnSO 47H 2O, 0.25mgNa 2MoO 42H 2O, 0.025mgCuSO 45H 2O, 0.025mgCoCl 26H 2O, 6000mgCH 3COOH and 24494mgCH 3COONa.So just can cultivate Belgium four seasons cuckoo.
Embodiment 2
Produce the green stem cutting bar of semi-lignified, the system fringe is handled plugged ear then with embodiment 1 described rooting promoter, and cuttage is in embodiment 1 described matrix again, and other is also with embodiment 1.

Claims (10)

1, the process for breeding and culturing of a kind of azalea comprises breeding and the cultivation of azalea, it is characterized in that wherein breeding used rooting promoter and contains indolebutyric acid, catechol and polyethylene pyrrolinone (PVP).
2, method according to claim 1 is characterized in that described rooting promoter is that every 100m1 rooting promoter contains 0.05-0.15g indolebutyric acid, 0.025-0.075g catechol, 0.06-0.15gPVP, 10-80ml ethanol, and all the other are water.
3, the method for claim 1 is characterized in that described rooting promoter further contains talcum powder.
4, method as claimed in claim 3 is characterized in that described rooting promoter prescription is that every 100g talcum powder is furnished with 0.5-1.6g indolebutyric acid, 0.25-0.75g catechol and 0.5-1.5gPVP.
5, the method for claim 1 is characterized in that wherein cultivating and is soilless culture, uses soilless substrate and nutrient solution.
6, method as claimed in claim 5 is characterized in that described nutrient solution is that every premium on currency is furnished with 413-1241mgCa (NO 3) 24H 2O, 60-180mgNH 4NO 3, 253-759mgKNO 3, 320-540mgNa 2HPO 4, 50-150mgPVP, 68-204mgKH 2PO 4, 246-740mgMgSO 47H 2O, 13.9-41.7mgFeSO 4, 18.6-55.8mgEDTANa 2, 3.1-9.3mgHBO 3, 11.1-33.5mgMnSO 44H 2O, 4.3-12.9mgZnSO 47H 2O, 0.12-0.38mg Na 2MoO 42H 2O, 0.012-0.038mgCuSO 45H 2O, 0.012-0.038mgCoCl 26H 2O, 3000-9000mg CH 3COOH and 12247-36741mgCH 3COONa.
7,, it is characterized in that wherein breeding comprising the steps: as the described method of one of claim 1 to 6
1) sets up the aseptic culture system;
2) induce the explant Growth and Differentiation;
3) promote adventitious buds proliferation;
4) strong sprout;
5) handle with rooting promoter;
6) root induction;
7) seedling management.
8, method as claimed in claim 7, the medium that it is characterized in that described aseptic culture system are that every liter of medium contains 413-1241mgCa (NO 3) 24H 2O, 30-500mgNa 2HPO 4, 60-180mg NH 4NO 3, 253-759mgKNO 3, 68-204mg KH 2PO 4, 246-740mgMgSO 47H 2O, 13.9-41.7mgFeSO 4, 18.6-55.8mgEDTANa 2, 3.1-9.3mgHBO 3, 11.1-33.5mgMnSO 44H 2O, 0.12-0.38mgNaMoO 42H 2O, 4.3-12.9mgZnSO 47H 2O, 0.012-0.038mgCuSO 45H 2O, 0.012-0.038mgCoCl 26H 2O, 0.41-1.25mgKI, 50-150mg inositol, 1-3mg glycine, 0.2-0.6mg thiamine hydrochloride (VB 1), 0-100 μ g), 3000-9000mg agar 5000-15000mg sucrose, zeatin (opens the beginning: 0.5-2mg, subculture: 0.2-1mg), (open the beginning: 10-100 μ g, subculture:, all the other are water to heteroauxin.
9,, it is characterized in that wherein breeding and comprise intercepting semi-lignified green stem cutting bar, system fringe, rooting promoter processing, root induction and seedling management as the described method of one of claim 1 to 6.
10, the described method of above-mentioned any one claim is used for breeding and cultivation Belgium four seasons cuckoo.
CN 94106373 1994-06-16 1994-06-16 Process for breeding and culturing azalea Pending CN1114129A (en)

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Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1100480C (en) * 2000-10-18 2003-02-05 贵州科学院 Quick vegetative propagation method for belgium azalea
CN1100479C (en) * 2000-05-12 2003-02-05 贵州科学院 Method for cultivating delaray rhodocndron
CN1108092C (en) * 2000-10-31 2003-05-14 贵州科学院 Cultivation technology for Belgium azalea and the nutritions soil thereof
CN104824033A (en) * 2015-05-12 2015-08-12 柳州市耕青科技有限公司 Special rooting agent for sabina virginiana
CN104824032A (en) * 2015-05-12 2015-08-12 柳州市耕青科技有限公司 Special rooting agent for juniperus chinensis
CN104872183A (en) * 2015-05-12 2015-09-02 柳州市耕青科技有限公司 Special rooting agent for cedars
CN104973972A (en) * 2015-07-06 2015-10-14 汤在英 Rhododendron soilless cultivation nutrient formula and application thereof
CN105052913A (en) * 2015-08-25 2015-11-18 何其家 Node using method of high-concentration BA gel
CN105052907A (en) * 2015-08-25 2015-11-18 钱夕华 Node position using method for low-concentration IBA and glycerin gel
CN105103884A (en) * 2015-08-25 2015-12-02 钱夕华 Low-concentration NAA and glycerin jelly node order using method
CN105103883A (en) * 2015-08-25 2015-12-02 钱夕刚 Method for applying high-concentration IAA gel to leaf positions
CN105103885A (en) * 2015-08-25 2015-12-02 何其家 Low-concentration BA and glycerin jelly node order using method
CN105123685A (en) * 2015-08-25 2015-12-09 何其家 Node order using method of medium-concentration BA gel
CN105191929A (en) * 2015-08-25 2015-12-30 钱家静 Section-position using method adopting high-concentration 2,4-D and glycerinum gel
CN105191917A (en) * 2015-08-25 2015-12-30 钱夕刚 Node position use method of low-concentration IAA and glycerogel
CN105191927A (en) * 2015-08-25 2015-12-30 钱家静 Section using method of middle-concentration 2,4-D and glycerin gel
CN105191928A (en) * 2015-08-25 2015-12-30 钱家静 Node position use method of low-concentration 2,4-D and glycerogel
CN105191926A (en) * 2015-08-25 2015-12-30 何其家 Section using method of middle-concentration BA and glycerin gel
CN106358898A (en) * 2016-08-25 2017-02-01 平顶山学院 Cutting cultivation method for improving the rooting rate of azalea
CN106718498A (en) * 2016-11-24 2017-05-31 枞阳县弘阳园林绿化有限公司 A kind of cuttage and seedling culture method of cuckoo
CN107047184A (en) * 2017-04-12 2017-08-18 广东德高信种植有限公司 Cocoa cuttage and seedling culture method
CN113767848A (en) * 2021-08-25 2021-12-10 山东省农业科学院 Efficient rooting method for rhododendron lapponicum tissue culture seedlings
CN116264886A (en) * 2021-12-17 2023-06-20 广州白云山和记黄埔中药有限公司 Rhododendron purpureus cutting seedling medium and seedling method
CN117581874A (en) * 2024-01-19 2024-02-23 北京林业大学 Composite plant growth regulator for cutting azalea and cutting method of azalea

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1100479C (en) * 2000-05-12 2003-02-05 贵州科学院 Method for cultivating delaray rhodocndron
CN1100480C (en) * 2000-10-18 2003-02-05 贵州科学院 Quick vegetative propagation method for belgium azalea
CN1108092C (en) * 2000-10-31 2003-05-14 贵州科学院 Cultivation technology for Belgium azalea and the nutritions soil thereof
CN104824033A (en) * 2015-05-12 2015-08-12 柳州市耕青科技有限公司 Special rooting agent for sabina virginiana
CN104824032A (en) * 2015-05-12 2015-08-12 柳州市耕青科技有限公司 Special rooting agent for juniperus chinensis
CN104872183A (en) * 2015-05-12 2015-09-02 柳州市耕青科技有限公司 Special rooting agent for cedars
CN104973972A (en) * 2015-07-06 2015-10-14 汤在英 Rhododendron soilless cultivation nutrient formula and application thereof
CN105123685A (en) * 2015-08-25 2015-12-09 何其家 Node order using method of medium-concentration BA gel
CN105191928A (en) * 2015-08-25 2015-12-30 钱家静 Node position use method of low-concentration 2,4-D and glycerogel
CN105103884A (en) * 2015-08-25 2015-12-02 钱夕华 Low-concentration NAA and glycerin jelly node order using method
CN105103883A (en) * 2015-08-25 2015-12-02 钱夕刚 Method for applying high-concentration IAA gel to leaf positions
CN105103885A (en) * 2015-08-25 2015-12-02 何其家 Low-concentration BA and glycerin jelly node order using method
CN105052913A (en) * 2015-08-25 2015-11-18 何其家 Node using method of high-concentration BA gel
CN105191929A (en) * 2015-08-25 2015-12-30 钱家静 Section-position using method adopting high-concentration 2,4-D and glycerinum gel
CN105191917A (en) * 2015-08-25 2015-12-30 钱夕刚 Node position use method of low-concentration IAA and glycerogel
CN105191927A (en) * 2015-08-25 2015-12-30 钱家静 Section using method of middle-concentration 2,4-D and glycerin gel
CN105052907A (en) * 2015-08-25 2015-11-18 钱夕华 Node position using method for low-concentration IBA and glycerin gel
CN105191926A (en) * 2015-08-25 2015-12-30 何其家 Section using method of middle-concentration BA and glycerin gel
CN106358898A (en) * 2016-08-25 2017-02-01 平顶山学院 Cutting cultivation method for improving the rooting rate of azalea
CN106718498A (en) * 2016-11-24 2017-05-31 枞阳县弘阳园林绿化有限公司 A kind of cuttage and seedling culture method of cuckoo
CN107047184A (en) * 2017-04-12 2017-08-18 广东德高信种植有限公司 Cocoa cuttage and seedling culture method
CN113767848A (en) * 2021-08-25 2021-12-10 山东省农业科学院 Efficient rooting method for rhododendron lapponicum tissue culture seedlings
CN116264886A (en) * 2021-12-17 2023-06-20 广州白云山和记黄埔中药有限公司 Rhododendron purpureus cutting seedling medium and seedling method
CN117581874A (en) * 2024-01-19 2024-02-23 北京林业大学 Composite plant growth regulator for cutting azalea and cutting method of azalea
CN117581874B (en) * 2024-01-19 2024-03-26 北京林业大学 Composite plant growth regulator for cutting azalea and cutting method of azalea

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