CN105191927A - Section using method of middle-concentration 2,4-D and glycerin gel - Google Patents

Abstract

The invention discloses a section using method of middle-concentration 2,4-D and glycerin gel, and belongs to the technical field of plant biology. The method includes the steps of preparing a 2,4-D mother solution with concentration of 1 mg/mL, diluting the mother solution into a middle-concentration 2,4-D working solution with concentration of 100 mg/L, preparing middle-concentration 2,4-D and glycerin gel confining liquid containing sodium carboxymethylcellulose with mass concentration of 1% and sodium alga acid with mass concentration of 8% through the working solution, inserting branches in a culture medium, and dropwise adding the gel confining liquid to axillary buds of the healthy and thick branches.

Description

The joint position using method of concentration 2,4-D and glycerogel in one

Technical field

In one, the joint position using method of concentration 2,4-D and glycerogel, belongs to plant biotechnology field.

Background technology

Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as 2,4-D, IBA, NAA, 2,4-D, GA ₃, 6-BA, KT, TDZ, TIBA, ethrel, B ₉, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.

Summary of the invention

In one, the joint position using method of concentration 2,4-D and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;

2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;

cuttage: branch is inserted in cultivation matrix;

axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.

The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.

embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description.

Embodiment

In one, the joint position using method of concentration 2,4-D and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;

2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;

cuttage: pot marigold branch is inserted in cultivation matrix;

axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch, after 20-30 days, axillary bud sprouting rate reaches more than 90%, and branch rooting rate reaches more than 80%.

Claims (1)

1. the joint position using method of concentration 2,4-D and glycerogel in a kind, is characterized in that, include hormone mother liquor in described method, working solution preparation, the preparation of hormone gel, cuttage, axillalry bud place drip, concrete operation step is as follows:

1. hormone mother liquor: precise weight be 0.1g 2,4-D with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be 2, the 4-D mother liquors of 1mg/mL;

2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;

cuttage: branch is inserted in cultivation matrix;

axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.