CN105211058A - The joint position using method of a kind of middle concentration IAA and glycerogel - Google Patents
The joint position using method of a kind of middle concentration IAA and glycerogel Download PDFInfo
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- CN105211058A CN105211058A CN201510525305.9A CN201510525305A CN105211058A CN 105211058 A CN105211058 A CN 105211058A CN 201510525305 A CN201510525305 A CN 201510525305A CN 105211058 A CN105211058 A CN 105211058A
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- Prior art keywords
- hormone
- iaa
- working solution
- concentration
- mother liquor
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Abstract
The invention discloses the joint position using method of a kind of middle concentration IAA and glycerogel, belong to plant biotechnology field.First the IAA mother liquor of 1mg/mL is prepared; This mother liquor is diluted to concentration IAA working solution in 100mg/L; Be concentration IAA and glycerogel confining liquid in 8% sodium alginate with sodium carboxymethylcellulose, the mass concentration that above-mentioned working solution preparation mass concentration is 1%; Branch is inserted in cultivation matrix; Gel confining liquid is dripped at the axillalry bud place of the sturdy branch of health.
Description
Technical field
A joint position using method for middle concentration IAA and glycerogel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A joint position using method for middle concentration IAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A joint position using method for middle concentration IAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: pot marigold branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch, after 20-30 days, axillary bud sprouting rate reaches more than 90%, and branch rooting rate reaches more than 80%.
Claims (1)
1. in, a joint position using method for concentration IAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525305.9A CN105211058A (en) | 2015-08-25 | 2015-08-25 | The joint position using method of a kind of middle concentration IAA and glycerogel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525305.9A CN105211058A (en) | 2015-08-25 | 2015-08-25 | The joint position using method of a kind of middle concentration IAA and glycerogel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105211058A true CN105211058A (en) | 2016-01-06 |
Family
ID=54980679
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525305.9A Pending CN105211058A (en) | 2015-08-25 | 2015-08-25 | The joint position using method of a kind of middle concentration IAA and glycerogel |
Country Status (1)
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| CN (1) | CN105211058A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519339A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for medium-concentration NAA and glycerin gel |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008146153A2 (en) * | 2007-06-01 | 2008-12-04 | Universita' Di Pisa | Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| CN103918650A (en) * | 2014-04-09 | 2014-07-16 | 河北省农林科学院昌黎果树研究所 | Growth regulator for improving fruit setting rate of peaches and regulating method |
-
2015
- 2015-08-25 CN CN201510525305.9A patent/CN105211058A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008146153A2 (en) * | 2007-06-01 | 2008-12-04 | Universita' Di Pisa | Method for biofertilization and bioprotection of vegetal material, in particular for in vitro propagation, protected cultivations, nurseries, and bacterial formulation for carrying out said method |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| CN103918650A (en) * | 2014-04-09 | 2014-07-16 | 河北省农林科学院昌黎果树研究所 | Growth regulator for improving fruit setting rate of peaches and regulating method |
Non-Patent Citations (1)
| Title |
|---|
| 张溃珍 等: "高分子•激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519339A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for medium-concentration NAA and glycerin gel |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20160106 |
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| WD01 | Invention patent application deemed withdrawn after publication |