CN105165813A - Node order application method of high concentration IAA and glycerin gel - Google Patents
Node order application method of high concentration IAA and glycerin gel Download PDFInfo
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- CN105165813A CN105165813A CN201510525215.XA CN201510525215A CN105165813A CN 105165813 A CN105165813 A CN 105165813A CN 201510525215 A CN201510525215 A CN 201510525215A CN 105165813 A CN105165813 A CN 105165813A
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- iaa
- hormone
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- mother liquor
- working solution
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Abstract
The invention discloses a node order application method of high concentration IAA and glycerin gel, and belongs to the technical field of plant biology. The application method comprises the following steps: at first, preparing a IAA mother liquor with a concentration of 1 mg/mL, diluting the mother liquor into a high-concentration IAA work fluid with a concentration of 500 mg/L; using the work fluid to prepare a high-concentration IAA and glycerin gel confining liquid, which contains sodium carboxymethyl cellulose with a mass concentration of 1% and sodium alginate with a mass concentration of 8%; inserting branches into cultivation matrix; and dropwise adding the gel confining liquid on axillary buds of healthy and strong branches.
Description
Technical field
A joint position using method for high concentration IAA and glycerogel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A joint position using method for high concentration IAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A joint position using method for high concentration IAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: pot marigold branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Claims (1)
1. a joint position using method for high concentration IAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Priority Applications (1)
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CN201510525215.XA CN105165813A (en) | 2015-08-25 | 2015-08-25 | Node order application method of high concentration IAA and glycerin gel |
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CN201510525215.XA CN105165813A (en) | 2015-08-25 | 2015-08-25 | Node order application method of high concentration IAA and glycerin gel |
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CN105165813A true CN105165813A (en) | 2015-12-23 |
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CN201510525215.XA Pending CN105165813A (en) | 2015-08-25 | 2015-08-25 | Node order application method of high concentration IAA and glycerin gel |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105519340A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for high-concentration NAA and glycerin gel |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1061885A (en) * | 1992-01-17 | 1992-06-17 | 北京林业大学 | High efficient growing promoter for root of plant |
CN102511488A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Plant sprouting agent and preparation method thereof |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525215.XA patent/CN105165813A/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1061885A (en) * | 1992-01-17 | 1992-06-17 | 北京林业大学 | High efficient growing promoter for root of plant |
CN102511488A (en) * | 2011-12-15 | 2012-06-27 | 河南科技大学 | Plant sprouting agent and preparation method thereof |
CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105519340A (en) * | 2016-01-08 | 2016-04-27 | 何晴雪 | Node order use method for high-concentration NAA and glycerin gel |
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Application publication date: 20151223 |
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