CN105519340A - Node order use method for high-concentration NAA and glycerin gel - Google Patents
Node order use method for high-concentration NAA and glycerin gel Download PDFInfo
- Publication number
- CN105519340A CN105519340A CN201610010238.1A CN201610010238A CN105519340A CN 105519340 A CN105519340 A CN 105519340A CN 201610010238 A CN201610010238 A CN 201610010238A CN 105519340 A CN105519340 A CN 105519340A
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- China
- Prior art keywords
- hormone
- naa
- concentration
- mother liquor
- working solution
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Cosmetics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a node order use method for high-concentration NAA and glycerin gel and belongs to the technical field of plant biology. The method comprises the steps: firstly, preparing 1mg/mL NAA mother liquor; diluting the mother liquor, so as to form 500mg/L high-concentration NAA working fluid; preparing high-concentration NAA and glycerin gel confining liquid, of which the mass concentration of sodium carboxymethyl cellulose is 1% and the mass concentration of sodium alginate is 8%, by using the working fluid; inserting branches into a culture substrate; and dropwise adding the gel confining liquid to axillary buds of healthy and robust branches.
Description
Technical field
A joint position using method for high concentration NAA and glycerogel, belongs to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as NAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
A joint position using method for high concentration NAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
A joint position using method for high concentration NAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: pot marigold branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Claims (1)
1. a joint position using method for high concentration NAA and glycerogel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
cuttage: branch is inserted in cultivation matrix;
axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610010238.1A CN105519340A (en) | 2016-01-08 | 2016-01-08 | Node order use method for high-concentration NAA and glycerin gel |
Applications Claiming Priority (1)
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CN201610010238.1A CN105519340A (en) | 2016-01-08 | 2016-01-08 | Node order use method for high-concentration NAA and glycerin gel |
Publications (1)
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CN105519340A true CN105519340A (en) | 2016-04-27 |
Family
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Family Applications (1)
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CN201610010238.1A Pending CN105519340A (en) | 2016-01-08 | 2016-01-08 | Node order use method for high-concentration NAA and glycerin gel |
Country Status (1)
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105052906A (en) * | 2015-08-25 | 2015-11-18 | 钱夕华 | Node using method of high-concentration IBA and glycerin gel |
CN105123688A (en) * | 2015-08-25 | 2015-12-09 | 何其家 | Node order using method of high-concentration KT and glycerine gel |
CN105123684A (en) * | 2015-08-25 | 2015-12-09 | 何其家 | Node order using method of high-concentration BA and glycerine gel |
CN105165813A (en) * | 2015-08-25 | 2015-12-23 | 钱夕刚 | Node order application method of high concentration IAA and glycerin gel |
CN105191929A (en) * | 2015-08-25 | 2015-12-30 | 钱家静 | Section-position using method adopting high-concentration 2,4-D and glycerinum gel |
-
2016
- 2016-01-08 CN CN201610010238.1A patent/CN105519340A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105052906A (en) * | 2015-08-25 | 2015-11-18 | 钱夕华 | Node using method of high-concentration IBA and glycerin gel |
CN105123688A (en) * | 2015-08-25 | 2015-12-09 | 何其家 | Node order using method of high-concentration KT and glycerine gel |
CN105123684A (en) * | 2015-08-25 | 2015-12-09 | 何其家 | Node order using method of high-concentration BA and glycerine gel |
CN105165813A (en) * | 2015-08-25 | 2015-12-23 | 钱夕刚 | Node order application method of high concentration IAA and glycerin gel |
CN105191929A (en) * | 2015-08-25 | 2015-12-30 | 钱家静 | Section-position using method adopting high-concentration 2,4-D and glycerinum gel |
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Application publication date: 20160427 |