CN105191919A - Low-concentration NAA and glycerogel root-dipping method - Google Patents
Low-concentration NAA and glycerogel root-dipping method Download PDFInfo
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- CN105191919A CN105191919A CN201510525417.4A CN201510525417A CN105191919A CN 105191919 A CN105191919 A CN 105191919A CN 201510525417 A CN201510525417 A CN 201510525417A CN 105191919 A CN105191919 A CN 105191919A
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Abstract
The invention discloses a low-concentration NAA and glycerogel root-dipping method, and belongs to the technical field of plant biology. The method comprises the steps that a 1 mg/mL NAA mother solution is prepared; the mother solution is diluted into a 10 mg/L low-concentration NAA working solution; low-concentration NAA and glycerogel confining liquid with the mass concentration of sodium carboxymethyl cellulose equal to 1% and the mass concentration of sodium alginate equal to 8% is prepared through the working solution; the lower ends of healthy and thick branches are dipped in the gel confining liquid for 3-5 seconds and taken out; the branches are inserted into a culture medium for rooting.
Description
Technical field
Low concentration NAA and glycerogel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as NAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
Low concentration NAA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
Low concentration NAA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in the described method of institute, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, simultaneously add 10ml glycerine according to 90ml hormone working solution;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: inserted in cultivation matrix by above-mentioned branch and take root, after 30-40 days, rooting rate reaches more than 75%.
Claims (1)
1. low concentration NAA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525417.4A CN105191919A (en) | 2015-08-25 | 2015-08-25 | Low-concentration NAA and glycerogel root-dipping method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525417.4A CN105191919A (en) | 2015-08-25 | 2015-08-25 | Low-concentration NAA and glycerogel root-dipping method |
Publications (1)
| Publication Number | Publication Date |
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| CN105191919A true CN105191919A (en) | 2015-12-30 |
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|---|---|---|---|
| CN201510525417.4A Pending CN105191919A (en) | 2015-08-25 | 2015-08-25 | Low-concentration NAA and glycerogel root-dipping method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409935A (en) * | 2016-01-08 | 2016-03-23 | 何晴雪 | High-concentration NAA and glycerol gel flower soaking method |
| CN105409934A (en) * | 2016-01-08 | 2016-03-23 | 何晴雪 | Method for soaking flowers through medium-concentration NAA and glycerin gel |
| CN105409936A (en) * | 2016-01-08 | 2016-03-23 | 何晴雪 | High-concentration NAA and glycerol gel root soaking method |
| CN105638646A (en) * | 2016-01-08 | 2016-06-08 | 何晴雪 | Root wetting method using middle-concentration NAA (Naphthalene Acetic Acid) and glycerin gel |
| CN108812309A (en) * | 2018-05-31 | 2018-11-16 | 山西省农业科学院果树研究所 | A kind of method of cuttage root-taking in virus-free basic potato seed preparation process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
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2015
- 2015-08-25 CN CN201510525417.4A patent/CN105191919A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
Non-Patent Citations (1)
| Title |
|---|
| 张溃珍等: "高分子•激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409935A (en) * | 2016-01-08 | 2016-03-23 | 何晴雪 | High-concentration NAA and glycerol gel flower soaking method |
| CN105409934A (en) * | 2016-01-08 | 2016-03-23 | 何晴雪 | Method for soaking flowers through medium-concentration NAA and glycerin gel |
| CN105409936A (en) * | 2016-01-08 | 2016-03-23 | 何晴雪 | High-concentration NAA and glycerol gel root soaking method |
| CN105638646A (en) * | 2016-01-08 | 2016-06-08 | 何晴雪 | Root wetting method using middle-concentration NAA (Naphthalene Acetic Acid) and glycerin gel |
| CN108812309A (en) * | 2018-05-31 | 2018-11-16 | 山西省农业科学院果树研究所 | A kind of method of cuttage root-taking in virus-free basic potato seed preparation process |
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Application publication date: 20151230 |
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