CN105104078A - High-concentration IBA and glycerogel root soaking method - Google Patents
High-concentration IBA and glycerogel root soaking method Download PDFInfo
- Publication number
- CN105104078A CN105104078A CN201510525361.2A CN201510525361A CN105104078A CN 105104078 A CN105104078 A CN 105104078A CN 201510525361 A CN201510525361 A CN 201510525361A CN 105104078 A CN105104078 A CN 105104078A
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- China
- Prior art keywords
- hormone
- iba
- concentration
- root
- working solution
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a high-concentration IBA and glycerogel root soaking method and belongs to the technical field of plant biology. According to the method, IBA mother liquor with the concentration being 1 mg/mL is prepared firstly; the mother liquor is diluted into high-concentration IBA working liquor with the concentration being 500 mg/L; high-concentration IBA and glycerogel confining liquid composed of, by mass, 1% of sodium carboxymethylcellulose and 8% of sodium alga acid is prepared through the working liquor; healthy and strong branch lower ends are soaked in the gel confining liquid for 3-5 s, then taken out and inserted into cultivation matrix to take root.
Description
Technical field
High concentration IBA and glycerogel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IBA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
High concentration IBA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
High concentration IBA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, simultaneously add 10ml glycerine according to 90ml hormone working solution;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Claims (1)
1. high concentration IBA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 500mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525361.2A CN105104078A (en) | 2015-08-25 | 2015-08-25 | High-concentration IBA and glycerogel root soaking method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525361.2A CN105104078A (en) | 2015-08-25 | 2015-08-25 | High-concentration IBA and glycerogel root soaking method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105104078A true CN105104078A (en) | 2015-12-02 |
Family
ID=54652424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525361.2A Pending CN105104078A (en) | 2015-08-25 | 2015-08-25 | High-concentration IBA and glycerogel root soaking method |
Country Status (1)
| Country | Link |
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| CN (1) | CN105104078A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173234A (en) * | 2017-07-18 | 2017-09-19 | 北京农学院 | A kind of method of the direct Rapid Rooting of tissue-cultured seedling |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5602177A (en) * | 1991-02-06 | 1997-02-11 | Hoechst Aktiengesellschaft | Formulations of deltamethrin |
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN101953290A (en) * | 2010-09-16 | 2011-01-26 | 南京农业大学 | Pear tree water culturing method |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525361.2A patent/CN105104078A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5602177A (en) * | 1991-02-06 | 1997-02-11 | Hoechst Aktiengesellschaft | Formulations of deltamethrin |
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN101953290A (en) * | 2010-09-16 | 2011-01-26 | 南京农业大学 | Pear tree water culturing method |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107173234A (en) * | 2017-07-18 | 2017-09-19 | 北京农学院 | A kind of method of the direct Rapid Rooting of tissue-cultured seedling |
| CN107173234B (en) * | 2017-07-18 | 2018-07-06 | 北京农学院 | A kind of method of the direct Rapid Rooting of tissue-cultured seedling |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151202 |