CN104996406A - Root wetting method of high-concentration IAA gel - Google Patents
Root wetting method of high-concentration IAA gel Download PDFInfo
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- CN104996406A CN104996406A CN201510525484.6A CN201510525484A CN104996406A CN 104996406 A CN104996406 A CN 104996406A CN 201510525484 A CN201510525484 A CN 201510525484A CN 104996406 A CN104996406 A CN 104996406A
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- gel
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- iaa
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a root wetting method of a high-concentration IAA gel, belonging to the technical field of plant biology. Firstly IAA mother solution of 1mg/mL is prepared; the mother solution is diluted into high-concentration IAA working solution of 500 mg/L; by using the working solution, high-concentration IAA gel confining liquid of sodium carboxymethylcellulose with the mass concentration of 1 percent and sodium alginate with the mass concentration of 8 percent is prepared; the lower end of a healthy, thick and solid branch is wetted in the gel confining liquid for 3 to 5 seconds, and then is taken out; the branch is inserted into a culture medium for rooting.
Description
Technical field
High concentration IAA gel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
High concentration IAA gel be stained with a method for root, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 500mg/L under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
High concentration IAA gel be stained with a method for root, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 500mg/L under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: inserted in cultivation matrix by above-mentioned branch and take root, after 20-30 days, rooting rate reaches more than 75%.
Claims (1)
1. high concentration IAA gel be stained with a method for root, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IAA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:2 the High concentration auxin working solution that concentration is 500mg/L;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 500mg/L under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525484.6A CN104996406A (en) | 2015-08-25 | 2015-08-25 | Root wetting method of high-concentration IAA gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525484.6A CN104996406A (en) | 2015-08-25 | 2015-08-25 | Root wetting method of high-concentration IAA gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104996406A true CN104996406A (en) | 2015-10-28 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525484.6A Pending CN104996406A (en) | 2015-08-25 | 2015-08-25 | Root wetting method of high-concentration IAA gel |
Country Status (1)
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| CN (1) | CN104996406A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347434A (en) * | 2017-08-31 | 2017-11-17 | 赵妙元 | A kind of winter creeper cuttage and seedling culture method |
| CN107372073A (en) * | 2017-08-31 | 2017-11-24 | 赵妙元 | One kind is used for winter creeper cuttage and seedling culture nutritive mud |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525484.6A patent/CN104996406A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
Non-Patent Citations (1)
| Title |
|---|
| 张溃珍等: "高分子·激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107347434A (en) * | 2017-08-31 | 2017-11-17 | 赵妙元 | A kind of winter creeper cuttage and seedling culture method |
| CN107372073A (en) * | 2017-08-31 | 2017-11-24 | 赵妙元 | One kind is used for winter creeper cuttage and seedling culture nutritive mud |
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Application publication date: 20151028 |
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