CN105052915A - Root dipping method of medium-concentration KT gel - Google Patents
Root dipping method of medium-concentration KT gel Download PDFInfo
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- CN105052915A CN105052915A CN201510525621.6A CN201510525621A CN105052915A CN 105052915 A CN105052915 A CN 105052915A CN 201510525621 A CN201510525621 A CN 201510525621A CN 105052915 A CN105052915 A CN 105052915A
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- concentration
- hormone
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- root
- mother liquor
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a root dipping method of medium-concentration KT gel, and belongs to the technical field of plant biology. The method comprises the steps that KT mother liquor with the concentration being 1mg/mL is prepared at first; the mother liquor is diluted into a medium-concentration KT working solution with the concentration being 100 mg/L; the working solution is used for preparing medium-concentration KT gel confining liquid comprising sodium carboxymethylcellulose with the mass concentration being 1% and the sodium alginate with the mass concentration being 8%; the lower end of a healthy and thick branch is dipped in the gel confining liquid for 3-5 s, and then the branch is taken out; the branch is inserted into a culture substrate for rooting.
Description
Technical field
Middle concentration KT gel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
Middle concentration KT gel be stained with a method for root, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of KT of 0.1g dissolves, be settled to 100mL, be the KT mother liquor of 1mg/mL with distilled water;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
Middle concentration KT gel be stained with a method for root, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of KT of 0.1g dissolves, be settled to 100mL, be the KT mother liquor of 1mg/mL with distilled water;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: inserted in cultivation matrix by above-mentioned branch and take root, after 20-30 days, rooting rate reaches more than 70%.
Claims (1)
1. in concentration KT gel be stained with a method for root, it is characterized in that, include in described method hormone mother liquor, working solution preparation, hormone gel preparation, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of KT of 0.1g dissolves, be settled to 100mL, be the KT mother liquor of 1mg/mL with distilled water;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 100mg/L under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525621.6A CN105052915A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of medium-concentration KT gel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525621.6A CN105052915A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of medium-concentration KT gel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105052915A true CN105052915A (en) | 2015-11-18 |
Family
ID=54482651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525621.6A Pending CN105052915A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of medium-concentration KT gel |
Country Status (1)
| Country | Link |
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| CN (1) | CN105052915A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
-
2015
- 2015-08-25 CN CN201510525621.6A patent/CN105052915A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| WO2015038917A1 (en) * | 2013-09-13 | 2015-03-19 | Fine Agrochemicals Ltd. | Plant growth regulating composition and methods for making and using same |
Non-Patent Citations (1)
| Title |
|---|
| 张溃珍等: "高分子·激素水凝胶生根膜制备与试验", 《林业实用技术》 * |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151118 |