CN105104081A - Root dipping method of medium-concentration KT and glycerogel - Google Patents
Root dipping method of medium-concentration KT and glycerogel Download PDFInfo
- Publication number
- CN105104081A CN105104081A CN201510525593.8A CN201510525593A CN105104081A CN 105104081 A CN105104081 A CN 105104081A CN 201510525593 A CN201510525593 A CN 201510525593A CN 105104081 A CN105104081 A CN 105104081A
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- China
- Prior art keywords
- hormone
- concentration
- root
- working solution
- mother liquor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Abstract
The invention discloses a root dipping method of medium-concentration KT and glycerogel, and belongs to the field of plant biotechnology. The method includes the steps that a 1 mg/mL KT mother solution is prepared first; the mother solution is diluted to form a 100 mg/L medium-concentration KT working solution; a medium-concentration KT and glycerogel blocking solution containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8% is prepared from the working solution; the lower ends of healthy and sturdy branches are dipped into the gel blocking solution for 3-5 s and then taken out; the branches are inserted into a cultivation matrix to root.
Description
Technical field
Middle concentration KT and glycerogel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IAA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
Middle concentration KT and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of KT of 0.1g dissolves, be settled to 100mL, be the KT mother liquor of 1mg/mL with distilled water;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
Middle concentration KT and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of KT of 0.1g dissolves, be settled to 100mL, be the KT mother liquor of 1mg/mL with distilled water;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, simultaneously add 10ml glycerine according to 90ml hormone working solution;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: inserted in cultivation matrix by above-mentioned branch and take root, after 30-40 days, rooting rate reaches more than 75%.
Claims (1)
1. in concentration KT and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight is, after the KOH of a little 1M of KT of 0.1g dissolves, be settled to 100mL, be the KT mother liquor of 1mg/mL with distilled water;
2. working solution preparation: it is concentration hormone working solution in 100mg/L that above-mentioned mother liquor is diluted to concentration according to the ratio of volume ratio 1:10, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 100mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525593.8A CN105104081A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of medium-concentration KT and glycerogel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525593.8A CN105104081A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of medium-concentration KT and glycerogel |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105104081A true CN105104081A (en) | 2015-12-02 |
Family
ID=54652427
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525593.8A Pending CN105104081A (en) | 2015-08-25 | 2015-08-25 | Root dipping method of medium-concentration KT and glycerogel |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105104081A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5703010A (en) * | 1991-02-06 | 1997-12-30 | Hoechst Aktiengesellschaft | Formulations of crop protection agents |
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
-
2015
- 2015-08-25 CN CN201510525593.8A patent/CN105104081A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5703010A (en) * | 1991-02-06 | 1997-12-30 | Hoechst Aktiengesellschaft | Formulations of crop protection agents |
| CN101238806A (en) * | 2008-03-14 | 2008-08-13 | 郑永昌 | Fruit tree protection glue for preventing fruit tree disease scar and wound generated by pruning from rot |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151202 |
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| WD01 | Invention patent application deemed withdrawn after publication |