CN105104080A - Low-concentration IBA and glycerin jelly root dip method - Google Patents
Low-concentration IBA and glycerin jelly root dip method Download PDFInfo
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- CN105104080A CN105104080A CN201510525513.9A CN201510525513A CN105104080A CN 105104080 A CN105104080 A CN 105104080A CN 201510525513 A CN201510525513 A CN 201510525513A CN 105104080 A CN105104080 A CN 105104080A
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- Prior art keywords
- hormone
- iba
- concentration
- working solution
- root
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a low-concentration IBA and glycerin jelly root dip method and belongs to the field of the plant biotechnology. The method comprises the steps of preparing a 1 mg/mL IBA mother solution, diluting the mother solution into a 10 mg/L low-concentration IBA working solution, preparing a low-concentration IBA and glycerin jelly confining solution by means of the working solution, dipping the lower ends of healthy and thick branches in the jelly confining solution for 3-5 sec, taking out the branches, and inserting the branches in a culture medium for rooting, wherein the low-concentration IBA and glycerin jelly confining solution contains sodium carboxymethylcellulose the mass concentration of which is 1% and sodium alga acid the mass concentration of which is 8%.
Description
Technical field
Low concentration IBA and glycerogel be stained with a method for root, belong to plant biotechnology field.
Background technology
Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as IBA, IBA, NAA, 2,4-D, GA
3, 6-BA, KT, TDZ, TIBA, ethrel, B
9, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.
Summary of the invention
Low concentration IBA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.
embodiment:
Below in conjunction with specific embodiment, the present invention is conducted further description.
Embodiment
Low concentration IBA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, simultaneously add 10ml glycerine according to 90ml hormone working solution;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: 8-10cm is long, healthy sturdy Chinese littleleaf box branch lower end sticks in 3-5 in above-mentioned gel confining liquid and takes out after second;
cuttage: inserted in cultivation matrix by above-mentioned branch and take root, after 30-40 days, rooting rate reaches more than 75%.
Claims (1)
1. low concentration IBA and glycerogel be stained with a method for root, it is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel in described method, be stained with root, cuttage, concrete operation step is as follows:
1. hormone mother liquor: precise weight be 0.1g IBA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the IBA mother liquor of 1mg/mL;
2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L, adds 10ml glycerine according to 90ml hormone working solution simultaneously;
3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate is that the hormone of 10mg/L and glycerine working solution dissolve for subsequent use by the concentration of 100mL under 50 DEG C of water bath condition;
be stained with root: branch lower end sturdy for health is sticked in 3-5 in above-mentioned gel confining liquid and take out after second;
cuttage: above-mentioned branch is inserted in cultivation matrix and takes root.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525513.9A CN105104080A (en) | 2015-08-25 | 2015-08-25 | Low-concentration IBA and glycerin jelly root dip method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510525513.9A CN105104080A (en) | 2015-08-25 | 2015-08-25 | Low-concentration IBA and glycerin jelly root dip method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105104080A true CN105104080A (en) | 2015-12-02 |
Family
ID=54652426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510525513.9A Pending CN105104080A (en) | 2015-08-25 | 2015-08-25 | Low-concentration IBA and glycerin jelly root dip method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105104080A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02273122A (en) * | 1989-04-12 | 1990-11-07 | Tetsuo Aragaki | Culture of plant and device therefor |
| JPH0398518A (en) * | 1989-09-12 | 1991-04-24 | Kyowa Kk | Nutritive solution culture of tree and device therefor |
| CN101953290A (en) * | 2010-09-16 | 2011-01-26 | 南京农业大学 | Pear tree water culturing method |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
| CN103155831A (en) * | 2011-12-10 | 2013-06-19 | 何寒 | Sustainable water and fertilizer conservation production technique for banana cultivation |
-
2015
- 2015-08-25 CN CN201510525513.9A patent/CN105104080A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02273122A (en) * | 1989-04-12 | 1990-11-07 | Tetsuo Aragaki | Culture of plant and device therefor |
| JPH0398518A (en) * | 1989-09-12 | 1991-04-24 | Kyowa Kk | Nutritive solution culture of tree and device therefor |
| CN101953290A (en) * | 2010-09-16 | 2011-01-26 | 南京农业大学 | Pear tree water culturing method |
| CN103155831A (en) * | 2011-12-10 | 2013-06-19 | 何寒 | Sustainable water and fertilizer conservation production technique for banana cultivation |
| CN102599161A (en) * | 2012-02-09 | 2012-07-25 | 濮阳市林业科学院 | Hormone hydrogel rooting film |
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Application publication date: 20151202 |