CN107873285B - Winter cuttage method for daphne giraldii florescence - Google Patents
Winter cuttage method for daphne giraldii florescence Download PDFInfo
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- CN107873285B CN107873285B CN201711226721.4A CN201711226721A CN107873285B CN 107873285 B CN107873285 B CN 107873285B CN 201711226721 A CN201711226721 A CN 201711226721A CN 107873285 B CN107873285 B CN 107873285B
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- 241001163443 Daphne giraldii Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 23
- 238000005520 cutting process Methods 0.000 claims abstract description 108
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 26
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000006013 carbendazim Substances 0.000 claims abstract description 26
- 238000005507 spraying Methods 0.000 claims abstract description 20
- 229940088597 hormone Drugs 0.000 claims abstract description 14
- 239000005556 hormone Substances 0.000 claims abstract description 14
- 238000007598 dipping method Methods 0.000 claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims abstract description 7
- 239000010451 perlite Substances 0.000 claims description 9
- 235000019362 perlite Nutrition 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 3
- 230000004083 survival effect Effects 0.000 abstract description 15
- 238000011835 investigation Methods 0.000 abstract description 6
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract description 2
- 239000010931 gold Substances 0.000 abstract description 2
- 229910052737 gold Inorganic materials 0.000 abstract description 2
- -1 is wide Chemical compound 0.000 abstract description 2
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 239000012286 potassium permanganate Substances 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 239000004576 sand Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 4
- 239000002689 soil Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 239000000758 substrate Substances 0.000 description 3
- 238000005034 decoration Methods 0.000 description 2
- 238000001784 detoxification Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 240000001899 Murraya exotica Species 0.000 description 1
- 235000008766 Murraya exotica Nutrition 0.000 description 1
- 235000009696 Murraya paniculata Nutrition 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
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- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention provides a winter cuttage method for daphne giraldii florescence, belonging to the field of asexual propagation methods for woody plant flowers, comprising the following steps: 1) selecting annual semi-lignified branches with buds as cuttings in a greenhouse in winter; 2) quickly dipping the cutting slips obtained in the step 1) in an IBA hormone solution for 5-15 s; 3) inserting the quickly dipped cutting shoots in the step 2) into a cutting medium of a seedbed; 4) and after cuttage, spraying the carbendazim solution every 1-3 d in the morning and at night. In the invention, after 1 month after cuttage, the investigation rooting rate can reach more than 85%, the cutting shoot adventitious roots are 1-8, the root length is about 5cm, the cutting shoot containing the buds can normally bloom, the leaves of the cutting seedling are thick, the gold edge is wide, and the ornamental property of the cutting seedling can be ensured; after the cutting survival, the seedling rate reaches more than 80 percent, and the pot culture survival rate reaches more than 95 percent.
Description
Technical Field
The invention belongs to the field of vegetative propagation methods of woody plant flowers, and particularly relates to a winter cuttage method for daphne giraldii florescence.
Background
The Jinbian Ruixiang is a flower in Nanchang city, also is 1 kind of flower in the evening, has light purple flower and strong fragrance, has the florescence of 1-3 months, has the advantages of having four advantages of fragrance, color, posture and charm in spring, is deeply loved by people at home and abroad, and has wide market prospect.
The daphne giraldii blossoms but does not seed, generally through cuttage propagation, it is suitable to act before and after the miscanthus or autumn, the temperature difference is small in the morning and evening, the new branch is semi-lignified, and the cuttage propagation heals and takes root quickly. When the cuttage is selected in summer, the cuttage is generally carried out by a water cutting method, the cuttage is inserted into water 1/3, the cuttage is straightened and fixed, the water content is kept unchanged, the roots can be rooted and survived within one month, and the rooting rate almost reaches 100%. If cuttage is carried out in winter, just in the flowering period, in order to prevent flowering and nutrient consumption, all buds are picked off during cuttage, and cuttage is carried out on a hotbed; if the buds are not removed, the normal flowering is ensured, and the survival rate of the daphne giraldii is low.
In the prior art, daphne giraldii is a fleshy root, the culture soil is most beneficial to the growth of daphne giraldii, the humus soil is loose and fertile, permeable and breathable, and has strong fertilizer and moisture retention capacity, and when 30% of fine sand is added into the daphne giraldii to be used as the culture soil, the seedling growth is very good, pond sludge is also used as a substrate, but the substrates are generally used for cuttage in spring and autumn, and the substrates used in winter are not described in relevant ways.
Based on the research, the cutting method which is used for normal flowering of the flower spike and high survival rate in the off-season cutting period in winter in the daphne giraldii florescence is not related in the prior art.
Disclosure of Invention
In view of the above, the present invention is directed to a method for cutting daphne giraldii florescence, the cutting survival rate is above 95%, the seedling rate is above 80%, and the cutting slips can normally bloom during the cutting period.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a winter cuttage method for daphne giraldii florescence, which comprises the following steps:
1) selecting annual semi-lignified branches with buds as cuttings in a greenhouse in winter;
2) quickly dipping the cutting slips obtained in the step 1) in an IBA hormone solution for 5-15 s;
3) inserting the quickly dipped cutting shoots in the step 2) into a cutting medium of a seedbed;
4) and after cuttage, spraying the carbendazim solution every 1-3 d in the morning and at night.
Preferably, the temperature in the greenhouse is controlled to be 8-12 ℃.
Preferably, the relative humidity in the greenhouse is controlled to be 60-75%.
Preferably, 3-5 whole leaves are reserved in the cutting slips in the step 1), and at least 2 buds are contained.
Preferably, the diameter of the cutting slips in the step 1) is 0.4-0.8 cm.
Preferably, the concentration of the IBA hormone solution in the step 2) is 150-300 mg/L.
Preferably, the cutting medium in step 3) is perlite.
Preferably, the density of the cuttage in the step 3) is 280-600 plants/m2。
Preferably, the carbendazim solution in the step 4) is 40-80 times of the carbendazim solution.
Preferably, the using amount of the carbendazim solution in the step 4) is 0.8-1.2L/m2。
The invention provides a winter cuttage method for daphne giraldii florescence, which takes a semi-lignified branch with a bud in the current year as a cutting shoot, can perform cuttage in the flowering season, the selected and produced cutting shoot can guarantee certain metabolic physiology such as photosynthesis, the stored matters in the cutting shoot can also provide partial nutrition required by flowering, the treatment of IBA hormone solution can promote the cutting shoot to form callus and adventitious roots, the cutting shoot can normally bloom during cuttage under the combined action of the selection and production of the cutting shoot and the multiple factors of the IBA hormone solution, the cutting shoot can become seedlings after 1 month of cuttage, the cuttage survival rate reaches more than 95%, the seedling rate reaches more than 80%, and the ornamental quality and the resistance of a female parent material can also be guaranteed.
Drawings
FIG. 1 shows the roots of a plant in the winter cutting of daphne giraldii florescence within one month;
FIG. 2 shows the bud blooming shape of daphne giraldii during the winter cutting in the flowering phase.
Detailed Description
The invention provides a winter cuttage method for daphne giraldii florescence, which comprises the following steps:
1) selecting annual semi-lignified branches with buds as cuttings in a greenhouse in winter;
2) quickly dipping the cutting slips obtained in the step 1) in an IBA hormone solution for 5-15 s;
3) inserting the quickly dipped cutting shoots in the step 2) into a cutting medium of a seedbed;
4) and after cuttage, spraying the carbendazim solution every 1-3 d in the morning and at night.
In the invention, when cuttage is carried out in winter in the daphne giraldii florescence, an annual semi-lignified branch with buds is selected as a cutting in a greenhouse. In the invention, the temperature of the greenhouse shed is preferably controlled to be 8-12 ℃, more preferably 9.5-11 ℃, and most preferably 10 ℃; the relative humidity of the greenhouse is preferably controlled to be 60-75%; the daphne giraldii nitsche is beneficial to growth and development of daphne giraldii nitsche and can reduce diseases at the temperature of about 10 ℃ and the relative humidity of 60-75%. In order to ensure the ornamental quality and resistance of the daphne giraldii nitsche, the source of the cutting slips can be detoxified seedlings of the daphne giraldii nitsche or daphne giraldii nitsche sold in the market, the cultivation time of the daphne giraldii nitsche sold in the market is preferably more than 2 years, more preferably 3 years.
In the embodiment of the invention, the daphne giraldii detoxification seedling is sourced from biological resource research institute of academy of sciences of Jiangxi province, and can be cultured by itself, and the culture method comprises the following steps: taking daphne giraldii detoxification test-tube plantlets with subculture period of more than 2 years as test materials, taking terminal buds as subculture materials, and selecting PP with MS + 0.05-0.1 mg/L during subculture333A culture medium; and (4) proliferating in an MS culture medium after subculture, wherein the preferable rooting temperature is 22-24 ℃, the illumination time is 11h/d, and the illumination intensity is 1500 lx.
In the invention, a detoxified seedling or commercially available Murraya paniculata is taken as a cutting mother tree, and a cutting shoot is collected, wherein the collection time of the cutting shoot is preferably winter, more preferably 1 to 2 months, and most preferably 1 month, middle and last; reserving 3-5 whole leaves for the cutting, and reserving 4 leaves for the cutting; the cutting ear at least comprises 2 buds, the cutting ear is provided with leaves, the leaves are provided with axillary buds, and the leaves can be subjected to photosynthesis after cutting; the axillary buds can provide nutrition due to vigorous metabolism, and the growth and development of plants are facilitated; the buds of the cutting slips are reserved, and the cutting slips can normally bloom during the cuttage of the daphne giraldii; the cutting slips are annual semi-lignified branches, and 2-year old branches or lignified branches are excluded; the diameter of the cutting is preferably 0.4-0.8 cm, more preferably 0.45-0.6 cm, and most preferably 0.5 cm; the preferable length of the cutting is 8-12 cm, more preferably 9-11 cm, and most preferably 10 cm. After the cutting slips are cut, the cutting slips are preferably divided into 20 cuttings per bundle.
And quickly dipping the cutting slips in IBA hormone solution for 5-15 s. In the invention, the concentration of the IBA hormone solution is preferably 150-300 mg/L, more preferably 180-220 mg/L, and most preferably 200 mg/L; the time for the quick dipping is more preferably 8 to 12 seconds, and most preferably 10 seconds.
And cutting the quickly dipped cutting slips into a cutting medium of a seedbed. In the invention, the seedbed is arranged in a greenhouse, and the length of the seedbed is preferably 8-12 m, more preferably 10 m; the width of the seedbed is preferably 0.8-1.2 m, and more preferably 1 m; the depth of the seedbed is preferably 35-50 cm, more preferably 38-45 cm, and most preferably 40 cm. In the invention, sand is filled in a space of the seedbed which is 20cm upwards from the bottom of the seedbed, a layer of sunshade net is covered on the seedbed, the space on the sunshade net is filled with sterilized cuttage matrix, and a drain pipe is arranged at the bottom of the seedbed.
In the invention, the seedbed comprises a cutting matrix, wherein the matrix is preferably an inorganic mineral matrix, more preferably perlite; the perlite can be used after being sterilized, and the sterilizing is preferably exposed in the sun for 24-72 h, more preferably 36-54 h, and most preferably 48 h; spraying potassium permanganate after the sun exposure, wherein the mass concentration of the potassium permanganate solution is 0.1-0.2%, and more preferably 0.15%; the usage amount of the potassium permanganate solution is preferably 20-30L per cubic meter of cuttage matrix, and more preferably 25L per cubic meter of cuttage matrix. In the invention, the perlite belongs to a light matrix, has good physical and chemical properties, is easy to move and is beneficial to stirring, and avoids diseases of mixed matrixes such as soil and the like.
In the invention, a cutting medium is loaded into a seedbed one week before cutting, and is sterilized by spraying carbendazim, wherein the carbendazim is preferably 300-500 times of liquid, and more preferably 400 times of liquid; the using amount of the carbendazim is preferably 1-3L per square meter, and more preferably 2L.
In the invention, the depth of the cuttage is preferablyIs 3-5 cm, more preferably 4 cm; the cutting density is preferably (3-8) cm x (3-8) cm, more preferably (4-6) cm x (4-6) cm, and most preferably 5cm x 5 cm; the cutting amount is preferably 400 plants/m2。
And after cuttage, spraying the carbendazim solution every 1-3 d in the morning and at night. In the invention, after cuttage, a carbendazim solution is sprayed every 2 days, the carbendazim solution is sprayed preferably once in the morning and at night, the carbendazim solution is preferably 40-80 times, more preferably 45-55 times and most preferably 50 times, and the spraying amount of the carbendazim solution is preferably 0.8-1.2L per square meter and more preferably 1L. In the invention, the carbendazim sprayed after cuttage can be used for disinfection and moisture preservation, thereby being beneficial to cuttage rooting and ensuring the survival rate.
In the invention, after 1 month after cuttage, the investigation rooting rate can reach more than 85%, the cutting shoot adventitious roots are 1-8, the root length is about 5cm, the cutting shoot containing the buds can normally bloom, the leaves of the cutting seedling are thick, the gold edge is wide, and the ornamental property of the cutting seedling can be ensured; after the cutting survival, the seedling rate reaches more than 80 percent, and the pot culture survival rate reaches more than 95 percent.
The winter cuttage method for daphne giraldii florescence provided by the invention is explained in detail with reference to the following examples, but the method is not to be construed as limiting the scope of the invention.
Example 1
Taking daphne giraldii detoxified seedlings as test materials, placing perlite disinfected by potassium permanganate after insolation into a seedbed filled with 20cm of high sand and a layer of sunshade net in a greenhouse in winter one week before cuttage, and spraying 2L of 400 times carbendazim solution per square meter;
1, 20, collecting 1-year-old semi-lignified bud-containing branches on daphne giraldii mother trees as cutting slips, reserving 4 leaves of the cutting slips containing 3 buds, quickly dipping the cutting slips in 200mg/L IBA hormone solution for 10s, and immediately cutting, wherein the cutting depth is about 4cm, and the cutting density is 5cm multiplied by 5 cm;
after cuttage, spraying 50 times of carbendazim solution every 2 days in the morning and at night, wherein the spraying amount is 1L/m2。
After one month of cuttage, the rooting rate is over 85 percent, the adventitious roots of the cutting slips are 6, the roots are 5cm long, the buds normally bloom as shown in figure 1, the transplanting seedling rate is over 80 percent, and the pot culture survival rate is over 95 percent.
Example 2
Taking daphne giraldii detoxified seedlings as test materials, placing perlite disinfected by potassium permanganate after insolation into a seedbed filled with 20cm of high sand and a layer of sunshade net in a greenhouse in winter one week before cuttage, and spraying 2L of 400 times carbendazim solution per square meter;
1, 20, collecting 1-year-old semi-lignified bud-containing branches on daphne giraldii mother trees as cutting slips, reserving 4 leaves of the cutting slips containing 2 buds, quickly dipping the cutting slips in 100mg/L IBA hormone solution for 5s, and immediately cutting, wherein the cutting depth is about 4cm, and the cutting density is 4cm multiplied by 6 cm;
after cuttage, spraying 50 times of carbendazim solution every 2 days in the morning and at night, wherein the spraying amount is 1L/m2。
After one month of cuttage, the investigation rooting rate reaches more than 85%, the cutting shoot has 6 adventitious roots and 5cm long roots, the buds normally bloom, the transplanting seedling rate is more than 80%, and the pot culture survival rate is more than 95%.
Example 3
Taking 3-year-old commercially available daphne giraldii nitsche as a test material, placing perlite sterilized by potassium permanganate into a seedbed filled with 20cm high sand and a layer of sunshade net in a greenhouse in winter one week before cuttage, and spraying 2L of 400-time carbendazim solution per square meter;
1, 20, collecting 1-year-old semi-lignified bud-containing branches on daphne giraldii mother trees as cutting slips, reserving 4 leaves of the cutting slips containing 2 buds, quickly dipping the cutting slips in 300mg/L IBA hormone solution for 15s, and immediately cutting, wherein the cutting depth is about 4cm, and the cutting density is 6cm multiplied by 4 cm;
after cuttage, spraying 50 times of carbendazim solution every 2 days in the morning and at night, wherein the spraying amount is 1L/m2。
After one month of cuttage, the investigation rooting rate reaches more than 85%, the cutting shoot has 6 adventitious roots and 5cm long roots, the buds normally bloom, the transplanting seedling rate is more than 80%, and the pot culture survival rate is more than 95%.
Example 4
Taking 3-year-old commercially available daphne giraldii nitsche as a test material, placing perlite sterilized by potassium permanganate into a seedbed filled with 20cm high sand and a layer of sunshade net in a greenhouse in winter one week before cuttage, and spraying 2L of 400-time carbendazim solution per square meter;
1, 20, collecting 1-year-old semi-lignified bud-containing branches on daphne giraldii mother trees as cutting slips, reserving 4 leaves of the cutting slips containing 2 buds, quickly dipping the cutting slips in 200mg/L IBA hormone solution for 10s, and immediately cutting, wherein the cutting depth is about 4cm, and the cutting density is 6cm multiplied by 4 cm;
after cuttage, spraying 50 times of carbendazim solution every 2 days in the morning and at night, wherein the spraying amount is 1L/m2。
After one month of cuttage, the investigation rooting rate reaches more than 85%, the cutting shoot has 6 adventitious roots and 5cm long roots, the buds normally bloom, the transplanting seedling rate is more than 80%, and the pot culture survival rate is more than 95%.
Blank control example
The method is the same as the cuttage method of the embodiment 1-4, and is different only in that the cutting slips are quickly dipped in water for 10s, and after one month of cuttage, the rooting rate is only 10% and the seedling rate is 5%.
Examples of the experiments
According to the conventional cuttage method, the cutting is carried out in spring at the non-flowering stage in spring, 2-3 half leaves are left in the cutting, and the medium such as pond sludge is used for cuttage, so that the seedling rate is 60 percent, and the pot culture survival rate is 80 percent.
According to the embodiments, the winter cuttage method for daphne giraldii florescence provided by the invention can be used for off-season cuttage in winter, the cuttage seedling rate is high, the ornamental shapes of the golden edge and the like are good, the investigation rooting rate reaches more than 85% after one month of cuttage, the cutting shoot has 1-8 adventitious roots and 5cm long roots, the buds normally bloom, the transplanting seedling rate is more than 80%, and the pot culture survival rate is more than 95%.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (5)
1. A winter cuttage method for daphne giraldii florescence comprises the following steps:
1) selecting annual semi-lignified branches with buds as cuttings in a greenhouse in winter;
2) quickly dipping the cutting slips obtained in the step 1) in an IBA hormone solution for 5-15 s;
3) inserting the quickly dipped cutting shoots in the step 2) into a cutting medium of a seedbed;
4) spraying carbendazim solution every 1-3 d in the morning and at night after cuttage;
the temperature in the greenhouse is controlled to be 8-12 ℃;
the relative humidity in the greenhouse is controlled to be 60-75%;
3-5 whole leaves are reserved on the cutting slips, and at least 2 buds are contained;
the concentration of the IBA hormone solution is 150-300 mg/L;
the cutting medium is perlite.
2. The method according to claim 1, wherein the diameter of the cutting in step 1) is 0.4-0.8 cm.
3. The method according to claim 1, wherein the density of the cuttings in step 3) is 280-600 plants/m2。
4. The method as claimed in claim 1, wherein the carbendazim solution in step 4) is 40-80 times liquid.
5. The method as claimed in claim 1 or 4, wherein the carbendazim solution of step 4) is used in an amount of 0.8-1.2L/m2。
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|---|---|---|---|---|
| CN104604661A (en) * | 2015-03-02 | 2015-05-13 | 吴国强 | Method for potting daphone odora varmargmata |
| CN105493832A (en) * | 2015-12-09 | 2016-04-20 | 重庆三零三科技有限公司 | Daphone odora Var. Marginata cultivation method |
| CN106961997A (en) * | 2017-05-06 | 2017-07-21 | 重庆辰央农业科技有限公司 | A kind of U.S.'s VC.Aureomariginatn cultural method |
| CN107027498A (en) * | 2017-05-19 | 2017-08-11 | 张灿芳 | A kind of family planting winter daphne technology |
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|---|---|---|---|---|
| CN104604661A (en) * | 2015-03-02 | 2015-05-13 | 吴国强 | Method for potting daphone odora varmargmata |
| CN105493832A (en) * | 2015-12-09 | 2016-04-20 | 重庆三零三科技有限公司 | Daphone odora Var. Marginata cultivation method |
| CN106961997A (en) * | 2017-05-06 | 2017-07-21 | 重庆辰央农业科技有限公司 | A kind of U.S.'s VC.Aureomariginatn cultural method |
| CN107027498A (en) * | 2017-05-19 | 2017-08-11 | 张灿芳 | A kind of family planting winter daphne technology |
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| Title |
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| 生长刺激素对金边瑞香扦插生根的效应研究;高柱 等;《江西科学》;20080430;第26卷(第2期);第231-233、294页 * |
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