CN103314748B - Seedling raising method of highbred honeysuckles - Google Patents

Seedling raising method of highbred honeysuckles Download PDF

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CN103314748B
CN103314748B CN201310223321.3A CN201310223321A CN103314748B CN 103314748 B CN103314748 B CN 103314748B CN 201310223321 A CN201310223321 A CN 201310223321A CN 103314748 B CN103314748 B CN 103314748B
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cuttage
seedling raising
honeysuckle
highbred
honeysuckles
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CN103314748A (en
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刘伟
王晓
刘大会
刘峰
耿岩玲
史国玉
周洁
刘建华
李圣波
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Shandong Analysis and Test Center
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Abstract

The invention discloses a seedling raising method of highbred honeysuckles by tender stem cutting. The seedling raising method includes steps that: non-lignified tender stems on stock plants of 1-year-old to 3-year-old highbred honeysuckles are selected and processed through growth hormones, cutting is performed with perlites serving as base materials in a greenhouse with adjustable temperature and adjustable humidity, roots grow in about two weeks, and the tender stems are transplanted to a big field for seedling hardening after 18-22 days. Compared with the prior art, the seedling raising method of the highbred honeysuckles has the advantages that the time of seedling raising by cutting is short, only two moths is needed from stem selection to big field transplanting, and the time is shortened by 60% compared with normal seedling raising; the seedling raising survival rate is very high and can reach 92%; the root system of seedlings cultured in the method is developed, and the new growing branches are sturdy and grow vigorously, grow up to seedlings and bloom in two years and are in full bloom in three years. The seedling raising method of the highbred honeysuckles leads breeding of the highbred honeysuckles not to be limited by regions and seasons, greatly shortens the seedling raising period and improves the seedling raising survival rate of the highbred honeysuckles.

Description

The seedling-cultivating method of a kind of breeding honeysuckle
Technical field
The present invention relates to the seedling-cultivating method of a kind of breeding honeysuckle, specifically a kind of selection of honeysuckle nursery cuttage branch, cuttage process and breeding method.
Background technology
Honeysuckle is traditional large traditional Chinese medicine and large outlet medicinal material, and main product is in the ground such as Shandong, Henan, and Shandong is as the main place of production of honeysuckle, and its planting area mainly concentrates on Shandong Linyi Prefecture and neighbouring districts and cities thereof.Total cultivated area reaches 300,000 mu.In long-term cultivation, Shandong honeysuckle creates very large variation in the Yield and quality of plant forms and medicinal material, and plantation colony mixes, old kind while degeneration immunity also in decline, thus cause damage by disease and insect to be on the rise, directly affect the industry development of honeysuckle.
Shandong Ya Te ecological technique Co., Ltd in 2010 success seed selection " Ya Te ", " sub-special red ", " Ya Teliben " 3 kinds, and have passed the breeding authorization of the forest variety certification committee of the State Administration of Forestry, finish the history that China does not have honeysuckle breeding.Along with the generation of breeding and the expansion gradually of honeysuckle cultivated area, how the demand expanding day of breeding honeysuckle seedling, accelerate breeding honeysuckle and breed and become problem demanding prompt solution on the basis ensureing its excellent genetic character.
Sub-special breeding honeysuckle is this undershrub vertical, and amount of growth doubles above compared with common honeysuckle kind, and its biological character is upright, multi-branched, and branch shape is sturdy, not obvious winding.Honeysuckle breeds the vegetative manner that seedling generally selects cuttage, the selection of survival rate and branch, the process of cultivation and condition have very big relation, therefore, in seedling raising process, the selection of the specification of breeding honeysuckle branch, training method and condition has survived great impact to seedling.
Through finding the literature search of prior art, Wang Huili is at " Shanxi Agricultural science " (Vol.40, No.3,2012,241 ~ 242) " research of honeysuckle Technique on Cuttage Propagation " has been delivered, the branch of the semi-lignified that research finds on selection 1 ~ 2 year raw honeysuckle easily produces callus, and power of taking root is strong.The selection of cuttage bar requires, for about length 10cm, generally to have the bud of more than 3, leave 2, cutting top leaflet, is soaked 2 hours bottom, then control solid carbon dioxide before cuttage in clear water, and base portion dips in No. 2, root treasured 3 hours, according to spacing in the rows 4cm cuttage.Rootage duration is 2 ~ 3 weeks, rooting rate highest order 80%, and cutting time is September.Wang Junming is at " Shaanxi forestry science and technology " (No.3,2005,81) delivered on " honeysuckle cuttage technique ", research finds that honeysuckle is hard, spray all can utilize husky matrix to carry out cuttage, process with No. ABT1 before cuttage, survival rate can reach 96.7%, and required time was 2 ~ 3 week, but cutting time is applicable on July, the middle ten days.Lan Afeng is at " Xibei Forest College's journal " (Vol.21, No.2,2006,93 ~ 96) " research of honeysuckle cuttage raise seedling technique " has been delivered on, research discovery selects 2 years raw 4mm thick ideal without leaf fringe bar, uses the IBA of 100mg/L leaching branch 30min survival rate the highest.Wang Wenjing is at " Shaanxi forestry science and technology " (No.3,2009,46 ~ 48) delivered on " experimental study of honeysuckle cuttage and seedling culture ", research finds that honeysuckle cutting time is good with 7 ~ August before entering the hottest period of the summer, seedling growing in summer takes cool canopy can improve honeysuckle emergence rate 10% ~ 15%, being up to 81.9% with the emergence rate of 1 year raw branch cuttings, is the key measure of guarantee fund's honeysuckle flower cuttage and seedling culture with inserting with watering permeable.Chen Xingfu is at " CHINA JOURNAL OF CHINESE MATERIA MEDICA " (Vol.33, No.11,2008,1240 ~ 1242) delivered on " impact that cutting time grows on gold and silver flower seedling ", research finds that cuttage in spring is conducive to surviving of honeysuckle, utilize the annotinous branch on perennial honeysuckle plant, each cuttage bar retains 2 joints, the joint of physiology upper end retains the branch of about 1cm, lower end retains the branch of about 2.5cm, February ~ cuttage by stages in March, March, most high-servival rate was 81.33%.Liao Lin " northern gardening " has delivered " growth regulatory substance and degree of lignification are on the impact of honeysuckle cutting propagation " (No.4,2012,161 ~ 163), study with leaflet honeysuckle as material, with the different degrees of lignification of different IBA, IBA and NAA mixing root-growing agents and fringe bar for research object, find that the IBA of 30mg/L is conducive to taking root of leaflet honeysuckle as root-growing agent and germinates, and degree of lignification all do not have too much influence to the rootage duration of honeysuckle and germinating time.
The application for a patent for invention that Chinese Patent Application No. is 200610050985.4, publication number is CN1820585A discloses a kind of high efficiency breeding technology for lonicera macranthoides honeysuckle, and object is to provide the propagation method of the lonicera macranthoides honeysuckle that a kind of seedling percent is high, cost is low.Technical scheme comprises adopts a garden sterilization, deinsectization and nursery bed disinfection, sterilization, adopts branch, beta pruning, cuttage, seedbed management and heeling in; In the wherein seedbed management stage, the relative moisture of pot-life and callus phase controls 60 ~ 80%, ground temperature control 25 ~ 28 DEG C, room temperature controls at 15 ~ 30 DEG C; The seedling percent of largeflower-like honeysuckle flower can reach more than 70%, and the cost of average every strain is only 0.8 yuan.
The patent of invention that China's number of applying for a patent is 201010113546.X, publication number is CN101766092A discloses the engrafting and cultivating method of a kind of honeysuckle, solution of the present invention is: 1, the cultivation of stock, the fruit adopting local wild varieties largeflower-like honeysuckle flower is bred, the largeflower-like honeysuckle flower seedling cultivated is got subterraneous root long 10 ~ 12cm, root diameter 0.5 ~ 1.5cm.Ground plant retains below 5cm, saves backup at 15 ~ 20 DEG C.2, the cultivation of grafting fringe bar, the branch getting the wild largeflower-like honeysuckle flower of life in 1 ~ 2 year, as grafting fringe bar, saves backup at 15 ~ 20 DEG C.3, enter resting stage honeysuckle, ready above-mentioned stock and grafting fringe strip adoption " cortex grafting " method are carried out grafting.4, implant soil cultivation after grafting to survive.The plant that the present invention cultivates is planted in 3 years on request just can reach high yield.
There is following defect or deficiency in above-mentioned technology: first, the above honeysuckle cuttage technique is all subject to the restriction of Various Seasonal, it is larger that cuttage in summer is subject to the such environmental effects such as temperature, rainfall, Autumn Cutting then needs the time oversize, want Second Year could sprout spring, and being subject to the impact of autumn and winter season strong wind, low temperature, cuttage seeding survival rate is unstable; The second, general cottage method all adopts fine sand as matrix, and water retention property is poor, and water requirement is large, and temperature difference variation obviously, is unfavorable for the growth of cuttage seeding new root; 3rd, the branch of the new semi-lignified sprouted then is all selected in the selection of general cuttage branch, and cambium is undeveloped, not easily take root or rootage duration longer, and lignified structure easily causes scattering and disappearing of moisture, cuttage seeding is dewatered dead.Therefore, along with the large-area popularization of breeding honeysuckle, in the urgent need to research and develop a kind of can large-scale promotion, affected by environment little, cultivate the cottage method that speed is fast and survival rate is high, to meet breeding honeysuckle demand to seedling in spread process.
Summary of the invention
For above-mentioned prior art, for overcoming deficiency of the prior art, the invention provides a kind of tender stem cuttage raise seedling technique and the perlite breeding method that are suitable for breeding honeysuckle fast breeding.The present invention is simple, and the cuttage survival rate of breeding honeysuckle can be made to improve 20%, and reach 92%, cutting time shortens 60%.
The present invention is achieved by the following technical solutions:
The tender stem cuttage and seedling culture method of a kind of breeding honeysuckle, step is as follows: select the tender stem of non-lignification in the breeder mother's strain in 1 ~ 3 year of breeding honeysuckle, after processing with somatotropin, cuttage is carried out using perlite as matrix, cuttage place is carried out in the greenhouse of adjustable temperature, humidity, take root in about 2 weeks, after 18 ~ 22 days, transplant to land for growing field crops hardening.
Preferably, select mother tree age 1 ~ 3 year, the non-lignification branch of the thick 2.05 ~ 4.32mm in footpath, most preferably mother tree age 2 years, thick about the 3mm in footpath, two joints, upper end have the non-lignification branch of two leaves.
Preferably, described somatotropin is IBA, and concentration for the treatment of is 100 mg/litre, and the leaching branch time is 30 ~ 40 minutes.
Preferably, described matrix (seedbed material) is thick 20cm, the perlite of wide 100cm, and further, automatic water sprinkler systme is established in its upper end, and sunshade net shelters from heat or light.
Preferably, described cuttage mode is: after cuttage seeding is ready to, vertical insertion seedbed 5 ~ 10cm, and seeding row spacing is 5cm, stays upper end two leaf, and timing water spray ensures that perlite water suction maintenance is saturated.
Preferably, after cuttage, control greenhouse temperature at 15 ~ 20 DEG C, humidity: perlite water suction keeps saturated; After 18 ~ 22 days (cuttage seeding is taken root), vertically plant into high 20cm by cuttage seeding according to seeding row spacing 5 ~ 10cm, on the seedbed, land for growing field crops of wide 100cm, hardening is pressed 1*1.5m seeding row spacing for 30 days afterwards and is transplanted to large Tanaka.
The tender stem cuttage and seedling culture method of breeding honeysuckle of the present invention, compared with prior art, has following useful effect: the cuttage and seedling culture time is short, from selecting branch only to need the time of 2 months to field-transplanting, shortens 60% than common seedling raise period; Survival rate of seedling superelevation, can reach 92%; The seedlings root of cultivating by the inventive method is flourishing, and a new branch is sturdy, and growing way is good, and in 2 years, rapid seedling is bloomed, 3 years Dasheng flower (table 5).The tender stem cuttage and seedling culture method of breeding honeysuckle of the present invention, makes breeding honeysuckle breed not by region, the restriction in season, substantially reduces growing-seedling period, improve honeysuckle survival rate of seedling.
Accompanying drawing explanation
Fig. 1: cuttage schematic diagram.
Fig. 2: hardening schematic diagram.
Embodiment
Below in conjunction with test and embodiment the present invention is further illustrated.
Test
By the following method and evaluation index test, obtain the best approach of breeding honeysuckle cuttage:
First be the selection of cuttage bar: select mother tree age 1 ~ 3 year, footpath slightly from the non-lignification of 2.05 ~ 4.32mm, semi-lignified and substantially lignified " Ya Te " breeding honeysuckle branch as cuttage bar.Experimental result is as shown in table 1, and experimental result shows, select mother tree age to be that 1 year footpath is slightly conducive to most surviving of cuttage bar at the non-lignification branch of about 3.00mm as cuttage bar, survival rate is about 92.5%.
The impact of table 1 cutting character pair cuttage seeding survival rate
Secondly, the selection of plant growth regulator, concentration and soak time: plant growth regulator is conducive to wound healing, this experimental study IBA, NAA, IAA, ABT, CK on cicatrizing date, phase of taking root, biomass of individual tree, survive the impact of seedling one-level side root length, cuttage survival rate etc.Result is as shown in table 2-table 4, and result shows, uses 100mgL -1iBA to soak effect after 30 ~ 40min best, cicatrizing date is the shortest, and within general about 2 weeks, can take root, cuttage seeding survival rate can reach 81%.The Asia of cuttage after 3 years special honeysuckle individual plant dried flower output is 0.78kg, and output is the 152.9%(table 5 of common leaflet honeysuckle).
Table 2 growth regulator is on the impact of cuttage bar survival rate
Table 3IBA concentration is on the impact of cuttage bar survival rate and growth
Table 4IBA soaks the root time to the impact of cuttage bar survival rate and growth
Table 53 year raw breeding honeysuckle increment inventory table
Cuttage seeding carries out cuttage after IBA soaks, and two leaves are stayed in cuttage branch upper end, and about 5cm in perlite seedling bed is inserted in lower end,
In this test, Cutting conditions is: cuttage spacing in the rows is about 2cm, and line-spacing is about 5cm, and after cuttage, water spray makes perlite water suction keep saturated, keeps warm indoor temperature to be about 15 ~ 20 DEG C, as shown in Figure 1.
In above-mentioned experimental basis, after 20 days, the honeysuckle seedling taken root is carried out hardening, hardening height of bed 20cm, wide 100cm, be vertically loaded into by seeding row spacing 5 ~ 10cm seedling of taking root, the hardening time is January, as shown in Figure 2, presses 1*1.5m seeding row spacing and transplant to large Tanaka after hardening.
The tender stem cuttage and seedling culture method of embodiment 1 breeding honeysuckle
Operating procedure is as follows:
(1) selection of cuttage bar: growth selection is vigorous, without 2 years raw breeding honeysuckles of damage by disease and insect, when shoot growth is to about 20cm, select non-lignification, footpath is slightly cut when about 3mm.Cuttage branch retains 2 internodes, and bud upper end, top and low side bud lower end retain 2 ~ 3cm respectively, and upper end is cut into flat mouth, and retain top 2 leaf, smooth ramp at 45 ° is cut in lower end, and length is 15 ~ 20cm.
(2) root is dipped in: by selecting every 100 strains of cuttage branch of having pruned to be bundled into 1 bundle, lower end is immersed in 100mgL -1iBA in, the time is 30min.
(3) cuttage: will the honeysuckle cuttage bar after root be dipped according to line-spacing 5cm, about spacing in the rows 2cm cuttage is to wide 100cm, in the perlite seedbed of thick 20cm, the cuttage degree of depth is about 5cm, compress after cuttage, irrigated by perlite by sprinkling irrigation system, keep perlite saturation moisture content, the temperature controlled in greenhouse is about 20 DEG C.Take root in 2 ~ 3 weeks, cuttage survival rate is 92%.
(4) hardening: after 20 days, the little spade of honeysuckle cuttage branch of taking root slowly is dug out from pearlite interstitial substance, transplant to being rich in organic sandy loam hardening Tanaka, Tanaka's ridging, ridge height 20cm, wide 100cm, according to seeding row spacing 10cm the seedling taken root vertically planted into, after planting, water spray irrigates.Transplant to land for growing field crops after hardening 30, according to the seeding row spacing field planting of 1*1.5m, planting survival rates is 90.4%.
The tender stem cuttage and seedling culture method of embodiment 2 breeding honeysuckle
Operating procedure is as follows:
(1) selection of cuttage bar: select not lignified 3 years raw honeysuckle cuttage branches, thick about the 3mm in cuttage bar footpath, cuttage branch retains 3 internodes, bud upper end, top and low side bud lower end retain 2 ~ 3cm respectively, upper end is cut into flat mouth, retain top 2 leaf, smooth ramp at 45 ° is cut in lower end, and length is 15 ~ 20cm.
(2) root is dipped in: by selecting every 100 strains of cuttage branch of having pruned to be bundled into 1 bundle, lower end is immersed in 100mgL -1iBA in, the time is 40min.
(3) cuttage: will the honeysuckle cuttage bar after root be dipped according to line-spacing 5cm, about spacing in the rows 2cm cuttage is to wide 100cm, in the perlite seedbed of thick 20cm, the cuttage degree of depth is about 5cm, compress after cuttage, irrigated by perlite by sprinkling irrigation system, keep perlite saturation moisture content, the temperature controlled in greenhouse is about 25 DEG C.Take root in 2 ~ 3 weeks, cuttage survival rate is 90%.
(4) hardening: after 20 days, slowly digs out the little spade of honeysuckle cuttage branch of taking root from pearlite interstitial substance, and transplants to being rich in organic hardening Tanaka, Tanaka's ridging, ridge height 20cm, wide 100cm, according to seeding row spacing 10cm the seedling taken root vertically planted into, after planting, water spray irrigates.Hardening was transplanted to land for growing field crops after 30 days, and according to the seeding row spacing field planting of 1*1.5m, planting survival rates is 88%.

Claims (1)

1. the seedling-cultivating method of a breeding honeysuckle, it is characterized in that: step is as follows: the tender stem of non-lignification selecting the thick 2.05 ~ 4.32mm in footpath in the breeder mother's strain in 1 ~ 3 year of breeding honeysuckle, after processing with somatotropin, cuttage is carried out using perlite as matrix, after cuttage, control greenhouse temperature at 15 ~ 20 DEG C, humidity: perlite water suction keeps saturated; After 18 ~ 22 days, vertically plant into high 20cm by the cuttage seeding of taking root according to seeding row spacing 5cm, on the seedbed, land for growing field crops of wide 100cm, hardening is pressed 1.5*1m seeding row spacing for 30 days afterwards and is transplanted to large Tanaka;
Described somatotropin is IBA, and concentration for the treatment of is 100 mg/litre, and the leaching branch time is 30 ~ 40 minutes;
Described matrix is the perlite of thick 20cm, wide 100cm;
Described cuttage mode is: tender for the non-lignification after somatotropin process penis and testes straight cutting is entered the seedbed 5 ~ 10cm using perlite as matrix, and seeding row spacing is 5cm, and the tender stem of non-lignification stays upper end two leaf, and timing water spray ensures that perlite water suction keeps saturated.
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CN104054481A (en) * 2014-06-09 2014-09-24 卞佳林 Cutting and seedling method of honeysuckle
CN105210621A (en) * 2015-09-24 2016-01-06 广西防城港市宏峰农林开发有限公司 A kind of seedling-cultivating method of white stone tooth tea
CN105532225A (en) * 2015-12-21 2016-05-04 江苏省中国科学院植物研究所 Cuttage propagating method for lonicera japonica
CN105638293A (en) * 2016-03-02 2016-06-08 临沂大学 Rapid seedling transplanting method of annual and biennial herbaceous flowers
CN106034935A (en) * 2016-06-22 2016-10-26 山东省潍坊市农业科学院 Amelanchier canadensis autumn twig cutting seedling method

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