CN113142060B

CN113142060B - Method for rapid and efficient propagation of Japanese beautyberry

Info

Publication number: CN113142060B
Application number: CN202110588987.3A
Authority: CN
Inventors: 朱恒星; 卢敏; 戴前莉; 黄飞逸; 陈本文; 高飞; 张晓蓉; 冉婷婷; 陶松
Original assignee: CHONGQING ACADEMY OF FORESTRY
Current assignee: CHONGQING ACADEMY OF FORESTRY
Priority date: 2021-05-28
Filing date: 2021-05-28
Publication date: 2022-06-07
Anticipated expiration: 2041-05-28
Also published as: CN113142060A

Abstract

The invention discloses a rapid and efficient propagation method of Japanese beautyberry, belonging to the technical field of Japanese beautyberry propagation and cultivation, and the propagation method comprises the following steps: (1) explant: selecting a callicarpa bodinieri bud which just sprouts in spring as an explant; (2) and (3) carrying out aseptic treatment on explants: removing all tissues except true leaves on the tender shoots for disinfection treatment; (3) primary culture: transferring the sterilized tender shoots into a primary culture medium; (4) subculturing: after a new contact surface is exposed, transferring the whole bud into a subculture medium, and transferring for 3 times to form cluster buds; (5) strengthening seedlings outside bottles and rooting integrated culture: cutting the strong successive cluster buds into single buds, putting a substrate and a gel nutrition sheet into a hole tray, inserting the single buds to be cut into the substrate by about 1cm by using a pair of tweezers, and then, completely pouring by using a rooting liquid, wherein the transparent film is fully covered; solves the problems of poor rooting quality of Japanese beautyberry and low survival rate of tissue culture seedlings caused by improper matching of a tissue culture method and a culture medium, shortens the culture period and reduces the culture cost.

Description

Method for rapid and efficient propagation of Japanese beautyberry

Technical Field

The invention relates to the technical field of Japanese beautyberry propagation and cultivation, in particular to a method for quickly and efficiently propagating Japanese beautyberry.

Background

Japanese beautyberry, academic name: callicarpa japonica thunb. var. japonica, Callicarpa, beautyberry, the name of English, beautyberry, is known as beautiful berry. The inflorescence is umbrella-shaped, light purple, the branches are soft and thin, the plant shape is fluffy, the autumn fruits are purple, the autumn fruits are glossy and very gorgeous, and each section grows uniformly, and the autumn fruits are strung continuously and can not fall off for a long time. The folium Callicarpae Formosanae extract has various excellent antioxidant components, and can inhibit the formation of mottle and resist skin aging.

Wild Japanese beautyberry seeds are greatly differentiated, and horticultural seeds are usually propagated by cutting, sowing and other modes, but the sowing survival rate is low, the growth vigor of cutting seedlings is relatively poor, the differentiation of seedling offspring is large, and the above propagation modes are not beneficial to the rapid propagation of Japanese beautyberry and the breeding and popularization of excellent single plants. The tissue culture technology can realize the high-efficiency and rapid breeding of the callicarpa bodinieri, save breeding materials such as spikes, fruits and the like, reduce the production cost of seedling culture, meet the ornamental requirement of market gardening, and simultaneously provide effective research materials for the pharmacological research of the drug property of the callicarpa bodinieri. Therefore, the Japanese beautyberry adopts a tissue culture mode to carry out rapid propagation, but the problem still exists in the tissue culture process.

In the process of Japanese beautyberry tissue culture, a culture medium is an important factor influencing the rooting rate and the survival rate of later-stage plants, the conditions of temperature, humidity, ventilation condition, nutrient content and the like of the medium directly influence the rooting rate and the rooting quality, the common medium at present takes vermiculite, river sand, perlite, humus, coconut shred and the like as mixed nutrient components, but if the component proportion is improper, the cuttage medium is not suitable, so that the early rooting cannot be promoted, the rooting quality is poor, and the tissue culture seedling dies. Therefore, in the rapid propagation process of Japanese beautyberry, the tissue culture method and the culture medium need to be comprehensively optimized, the quality of tissue culture seedlings is improved, and the rooting rate and the survival rate are obviously improved.

Disclosure of Invention

In view of the above, the invention aims to provide a method for rapid and efficient propagation of callicarpa japonica, which solves the problems of poor rooting quality of callicarpa japonica and low survival rate of tissue culture seedlings caused by improper matching of a tissue culture method and a culture medium.

The invention solves the technical problems by the following technical means:

a method for rapid and efficient propagation of Japanese beautyberry comprises the following steps:

(1) explant: selecting a callicarpa bodinieri bud which just sprouts in spring as an explant;

(2) and (3) carrying out aseptic treatment on explants: stripping off all tissues except true leaves on the tender buds, and disinfecting with soapy water, sterile water and mercuric chloride Tween disinfectant;

(3) primary culture: transferring the sterilized tender shoots into a primary culture medium, culturing 1 shoot in each bottle for 30 days;

(4) subculturing: growing single bud to 2-4cm, removing the contact surface between the bottom callus and the culture medium, exposing new contact surface, transferring the whole bud into subculture medium, and transferring for 3 times to form cluster bud;

(5) strengthening seedlings outside the bottle and rooting integrated culture: cutting strong subculture clumpy buds into single buds with the length of 2-3cm, performing out-bottle strong seedling and rooting integrated culture, and continuously transferring the bottom of the single buds into subculture, wherein the specific integrated culture method comprises the following steps:

uniformly mixing sterile fine coconut bricks, perlite and fine river sand according to a volume ratio of 3:1:0.5 to obtain a matrix, filling the matrix and gel nutrition tablets into a hole tray, watering by adopting 1 ‰ carbendazim until the matrix is kneaded into a mass without dripping water, transferring the single bud incision to be cut into the hole tray after micro-drying, inserting the single bud incision into the matrix by using a forceps for about 1cm, then completely watering by using rooting liquid, and fully covering by using a transparent film; and in the first 5 days, removing the film for ventilation after the film is full of water drops, shaking off the water drops on the film, removing the film for 30min every day after 5 days, removing the film for 1h in the morning and afternoon after 10 days, removing the film completely in the daytime after the length of a new root growing is 0.5cm, covering the film at night, spraying 1% compound fertilizer after new leaves grow out, and normally maintaining after removing the film completely.

Further, the gel nutritional tablet is soaked in water for 20-30min when in use.

In the conventional strong seedling and rooting culture process, phosphorus and potassium fertilizers are usually required to be poured before new leaves grow, so that the roots of callicarpa bodinieri seedlings which are not drought-tolerant and water-fast are easy to rot and the propagation fails. Therefore, the conventional strong seedling rooting process is improved, the gel nutrient tablet is adopted to enable the root seedlings to directly absorb fertilizer and divide water, the propagation and development of the Japanese beautyberry seedlings are more suitable, and the gel nutrient tablet is non-toxic and easy to decompose and is more suitable for being used during seedling cultivation. In addition, the out-of-bottle strong seedling rooting integrated culture is adopted, the culture process of rooting in a tissue culture bottle is omitted, the seedling culture period of strong seedling rooting and seedling hardening is shortened, and the seedling culture cost is reduced.

Further, before the explant is picked, 0.1wt% of thiophanate methyl is sprayed in the morning and at the evening of a sunny day for more than 3 continuous days in the early winter, and after the thiophanate methyl is sprayed for 3 days, the callicarpa formosana tender shoots which are not unfolded on leaves are picked as the explant by avoiding burning days and dew.

Further, in the explant aseptic treatment, flushing dust on the surface of the explant by flowing water, then washing the surface of the explant by 1 per thousand of soap water for 1min in a shaking way, then flushing the surface of the explant by flowing water for 15min, transferring the explant to an ultraclean workbench for 3 times in aseptic water, transferring the explant to 1 per thousand of mercuric chloride Tween disinfectant for shaking and disinfection for 6min after the moisture is filtered out by aseptic filter paper, and then rinsing the explant by aseptic water for three times to filter out the moisture.

Further, the formula of each liter of primary culture medium is as follows: MS +6BA 2.5mg/L + NAA 0.2mg/L + agar 6mg/L + white sugar 30 g/L.

Further, the formula of each liter of subculture medium is as follows: MS +6BA 1.5mg/L + NAA 0.1mg/L + vitamin h1.5mg/L + agar 6mg/L + white sugar 30 g/L.

Further, the formula of each liter of rooting solution is as follows: 1/2MS + IBA 0.5mg/L + NAA 0.1 mg/L.

Further, in the primary culture, the secondary culture, the out-of-bottle strong seedling and rooting integrated culture, the illumination intensity is 1800Lux, the illumination time is 12h/d, and the culture temperature is 23-27 ℃.

Further, when the substrate is arranged in the hole tray, partial substrate is arranged in the hole tray, then the gel nutrition piece is paved, and then a layer of substrate with the thickness of 1-2cm is paved on the gel nutrition piece.

Further, the gel nutrition tablet comprises the following raw materials:

30-50 parts of Shuo' e starch, 10-15 parts of sodium lignosulfonate, 15-18 parts of pectin, 5-10 parts of sodium trimetaphosphate and 8-12 parts of monopotassium phosphate.

Further, the preparation method of the gel nutrition tablet comprises the following steps:

modification of starch for large stone oil: mixing the starch for the major of the curcuma zedoary into 45-50% of starch milk by mass percent, adding sodium lignosulphonate, stirring at a high speed of 1000r/min for 10-20min at 800-;

pasting modification: stirring the standing modified starch for 10-30min, adding pectin, stirring for 10-20min, stopping heating, adding saturated water solution of potassium dihydrogen phosphate solution, stirring, adding sodium trimetaphosphate, heating to 70-80 deg.C, gelatinizing, and keeping the temperature for 20 min;

preparing gel nutrient tablets: pouring the gelatinized and modified starch into a mold, freezing in a refrigerator at 4 ℃ for 10-12h, taking out, placing in a hot air drier, and air-drying in circulating hot air at 50-60 ℃ for 30-40min to obtain the gel nutrition tablet.

Further, the thickness of the gel nutrition tablet is 1-2cm, and preferably 1.5 cm. The optimal thickness of the gel nutrition tablet is about 1.5cm, when the thickness of the gel nutrition tablet is too thick, water accumulation can be caused, the gel nutrition tablet is difficult to penetrate into a substrate for a long time, root rot can be caused, when the thickness of the gel nutrition tablet is too thin, water storage can be caused to be insufficient, and the nutrition and water content for maintaining normal growth of the callicarpa bodinieri can not be achieved.

The gel sheet prepared from the conventional starch has low stability, and is easy to mildew when being buried in a planting matrix, and seedlings are rotten and smelly due to bacteria growth, so that the Shuomie starch without toxic and side effects is selected as a main gel matrix when the gel nutrition sheet is prepared, and is modified, so that the gel nutrition sheet is buried in the matrix for a long time and does not mildew, can store enough nutrition for seedling growth and moisture to avoid frequent watering and fertilization, and the gel strength is increased, so that the gel nutrition sheet is convenient to use and prepare, and is more suitable for cultivating Japanese beauty-berry which is neither drought-endurable nor waterlogging-endurable in strong seedlings and rooting stages.

Has the advantages that:

the method for breeding the Japanese beautyberry solves the problems of poor rooting quality of the beautyberry and low survival rate of tissue culture seedlings caused by improper matching of a tissue culture method and a matrix, and simultaneously solves the problems of frequent watering and strict control of the watering quantity due to drought and waterlogging intolerance of the beautyberry, simplifies the operation, saves the labor, shortens the seedling culture period, improves the breeding speed, further improves the survival rate of the beautyberry, improves the quality of tissue culture seedlings, and is favorable for promoting the rapid breeding and popularization of excellent Japanese beautyberry varieties.

Drawings

FIG. 1: the picture of the proliferation bud in the proliferation culture stage of the invention;

FIG. 2: the picture of the cluster buds before the integrated culturing stage of strong seedling and rooting is transferred;

FIG. 3: the invention is a callicarpa seedling picture in the integral culture stage of strong seedlings and rooting outside the bottle;

FIG. 4: the picture of the gel sheet of the comparative example of the present invention was mildewed.

Detailed Description

The invention will be described in detail with reference to specific embodiments and drawings, wherein:

example 1:

the preparation method of the gel nutrient tablet used in the stages of strong seedling and rooting culture is as follows:

firstly, 30g of Shuo zedoary starch, 10g of sodium lignosulphonate, 15g of pectin, 5g of sodium trimetaphosphate and 8g of potassium dihydrogen phosphate are weighed.

The preparation method comprises the following steps:

modification of starch for large stone oil: mixing the starch for the major of the curcuma zedoary into starch milk with the mass fraction of 45%, adding sodium lignosulphonate, stirring at a high speed of 900r/min for 15min, and standing at normal temperature for 12h overnight;

pasting modification: stirring the stood modified Shuoang starch uniformly again, heating the starch in water bath at 40 ℃ for 20min, adding pectin, stirring for 15min, stopping heating after stirring is finished, preparing potassium dihydrogen phosphate into a saturated solution, adding the saturated solution into a starch solution, stirring uniformly, adding sodium trimetaphosphate, stirring uniformly, heating to 75 ℃ for gelatinization, and preserving heat for 20 min;

preparing gel nutrient tablets: pouring the gelatinized and modified starch into a mold, freezing for 11h in a refrigerator at 4 ℃, taking out, placing in a hot air drier, and air-drying in circulating hot air at 50 ℃ for 30min to obtain the gel nutrition tablet with the thickness of 1.5 cm.

Example 2:

firstly, 40g of Shuoyou starch, 12g of sodium lignosulfonate, 16g of pectin, 8g of sodium trimetaphosphate and 10g of potassium dihydrogen phosphate are weighed.

The preparation method is the same as that of example 1.

Example 3:

firstly, 50g of Shuo zedoary starch, 15g of sodium lignosulphonate, 18g of pectin, 10g of sodium trimetaphosphate and 12g of potassium dihydrogen phosphate are weighed.

The preparation method is the same as that of example 1.

Comparative example 1:

a comparative example was formed with example 1, and the raw materials were weighed according to the following weight: 30g of Shuoyou starch, 15g of pectin, 5g of sodium trimetaphosphate and 8g of potassium dihydrogen phosphate.

The preparation method comprises the following steps:

mixing starch of large diameter of Curcuma into 45 wt% starch milk, heating in water bath at 40 deg.C for 20min, adding pectin, stirring for 15min, stopping heating after stirring, making potassium dihydrogen phosphate into saturated solution, adding starch solution, stirring, adding sodium trimetaphosphate, stirring, heating to 75 deg.C, gelatinizing, and keeping the temperature for 20 min;

Comparative example 2:

a comparative example was formed with example 1, and the raw materials were weighed according to the following weight: 30g of Shuoye starch, 10g of sodium lignosulphonate, 5g of sodium trimetaphosphate and 8g of potassium dihydrogen phosphate.

The preparation method comprises the following steps:

mixing the starch for the major of the curcuma zedoary into starch milk with the mass fraction of 45%, adding sodium lignosulphonate, stirring at a high speed of 900r/min for 15min, and standing at normal temperature for 12h overnight; preparing saturated solution of potassium dihydrogen phosphate, adding into starch solution, stirring, adding sodium trimetaphosphate, stirring, heating to 75 deg.C, gelatinizing, and keeping the temperature for 20 min; preparing gel nutrient tablets: pouring the gelatinized and modified starch into a mold, freezing for 11h in a refrigerator at 4 ℃, taking out, placing in a hot air drier, and air-drying in circulating hot air at 50 ℃ for 30min to obtain the gel nutrition tablet with the thickness of 1.5 cm.

Comparative example 3:

a comparative example was formed with example 1, and the raw materials were weighed according to the following weight: 30g of Shuoye starch, 10g of sodium lignosulphonate, 15g of pectin and 8g of potassium dihydrogen phosphate.

The preparation method comprises the following steps:

mixing the Shuoyou starch into 45 mass percent starch milk, adding sodium lignosulphonate, stirring at high speed of 900r/min for 15min, and standing at normal temperature for 12h overnight; stirring the starch of modified Shuou zedoary after standing, heating in water bath at 40 deg.C for 20min, adding pectin, stirring for 15min, stopping heating after stirring, making potassium dihydrogen phosphate into saturated solution, adding into starch solution, stirring, heating to 75 deg.C for gelatinizing, and keeping the temperature for 20 min; pouring the gelatinized and modified starch into a mold, freezing for 11h in a refrigerator at 4 ℃, taking out, placing in a hot air drier, and air-drying in circulating hot air at 50 ℃ for 30min to obtain the gel nutrition tablet with the thickness of 1.5 cm.

Comparative example 4:

a comparative example was formed with example 1, weighing the following raw materials by weight: 30g of chitosan, 400ml of 2 wt% glutaraldehyde, 200ml of 2 wt% acetic acid solution, and 8g of potassium dihydrogen phosphate.

The preparation method comprises the following steps:

mixing chitosan and acetic acid solution, stirring for dissolving, adding glutaraldehyde, stirring for 10min, adding potassium dihydrogen phosphate, stirring at constant temperature of 50 deg.C for 1h, pouring into a mold, and standing overnight to obtain gel sheet with thickness of 1.5 cm.

Blank control:

weighing 30g of Shuo zedoary starch and 8g of potassium dihydrogen phosphate.

Mixing the starch for large diameter with 45 wt% of starch milk, adding potassium dihydrogen phosphate, stirring, heating to 75 deg.C for gelatinization, and keeping the temperature for 20 min; pouring the gelatinized starch into a mold, freezing in a refrigerator at 4 deg.C for 11h, taking out, placing in a hot air drier, and air drying in 50 deg.C circulating hot air for 30min to obtain gel nutritional tablet with thickness of 1.5 cm.

Soaking the gel sheet in water for 20min, testing water content at room temperature under 75% humidity, recording data of 1 day, 3 days, 5 days, 10 days, and 15 days, and obtaining data shown in Table 1:

TABLE 1 Water content (%)

	Example 1	Example 2	Example 3	Comparative example 1	Comparative example 2	Comparative example 3	Comparison ofExample 4	Blank group
									Initial value	100	100	100	100	100	100	100	100
1 day	98	99	98	95	92	99	87	95
									3 days	92	93	92	89	83	98	74	86
5 days	83	85	84	81	72	96	51	74
									10 days	67	68	69	66	61	94	24	53
15 days	42	45	47	46	50	92	9	27

Example 4: propagation of Callicarpa japonica

(1) Explant: before the explants are picked, continuously spraying 0.1wt% of thiophanate methyl in the morning and at the evening of more than 3 consecutive days in the end of winter and in the beginning of spring, after spraying for 3 days, avoiding burning sun and dew at about 9:00 in the morning, and picking the tender buds of the callicarpa bodinieri which are not unfolded and just bud in spring as the explants;

(2) and (3) carrying out explant sterile treatment: removing all tissues except true leaves on the tender buds, washing the surface dust of the explant by flowing water, shaking and cleaning for 1min by 1 thousandth of soapy water, then washing for 15min by flowing water, transferring to an ultraclean workbench, washing for 3 times by using sterile water, shaking and sterilizing for 6min by transferring to 1 thousandth of mercuric chloride Tween disinfectant after filtering water by using sterile filter paper, then rinsing for three times by using sterile water, and filtering water for later use;

(3) primary culture: transferring the sterilized tender shoots into a primary culture medium, wherein 1 shoot is cultured in each bottle for 30 days, and the formula of each liter of the primary culture medium is as follows: MS +6BA 2.5mg/L + NAA 0.2mg/L + agar 6mg/L + white sugar 30 g/L;

(4) subculturing: the single bud grows to 2-4cm, the contact surface of the bottom callus and the culture medium is removed, the whole bud is transferred to a subculture medium after a new contact surface is exposed, and the formula of each liter of the subculture medium is as follows: MS +6BA 1.5mg/L + NAA 0.1mg/L + vitamin h1.5mg/L + agar 6mg/L + white sugar 30g/L, transferring for 3 times to form cluster buds;

(5) strengthening seedlings outside the bottle and rooting integrated culture: cutting the strong subculture cluster buds into single buds with the length of 2-3cm, performing out-of-bottle strong seedling and rooting integrated culture, and continuously transferring the bottoms of the single buds to subculture;

the specific method for integrated strong seedling and rooting culture comprises the following steps:

uniformly mixing sterile fine coconut tiles, perlite and fine river sand according to a volume ratio of 3:1:0.5 to obtain a matrix, soaking a gel nutrition sheet in water for 20min, firstly filling part of the matrix into a hole tray, then paving the soaked gel nutrition sheet, then paving a layer of matrix with the thickness of 1.5cm on the gel nutrition sheet, watering 1 ‰ carbendazim after the hole tray is filled until the matrix is kneaded into a mass but does not drip, respectively transferring the cut of a single bud to the hole tray after the cut of the single bud is micro-dried, inserting the single bud into the matrix at the position of 1cm by using a pair of tweezers, then completely pouring a rooting solution, fully covering a transparent film, wherein the formula of each liter of the rooting solution is as follows: 1/2MS + IBA 0.5mg/L + NAA 0.1 mg/L;

5 days before the culture, when the film is full of water drops, uncovering the film to ventilate, shaking off the water drops on the film, uncovering the film for 30min every day after 5 days after the culture, uncovering the film for 1h respectively in the morning and afternoon after 10 days after the culture, after the length of a new root growing is 0.5cm, completely uncovering the film in the daytime, covering the film at night, spraying 1% compound fertilizer after new leaves grow out, and normally maintaining after completely uncovering the film.

In the primary culture, the secondary culture, the out-of-bottle strong seedling and the rooting integrated culture, the illumination intensity is 1800Lux, the illumination time is 12h/d, and the culture temperature is 25 +/-2 ℃.

Example 4 the nutritional gel tablets prepared in example 1 were used, while for comparison, gel tablets of comparative examples 1-4 and a blank set, which were different from the gel tablets used in example 4 only in the well plate, were also subjected to the Japanese beautyberry propagation experiment. 30 single shoots were inserted into each well plate, and the survival rate and growth of Japanese beautyberry were observed after 20 days of cultivation, and whether the gel pieces were mildewed or not was observed, and the data obtained are shown in Table 2.

TABLE 2

From the data analysis of table 1 and table 2, it can be seen that:

(1) the water content of the gel nutritional tablets prepared in examples 1-3 decreased by about 2% after 1 day, by about 8% after 3 days, by about 17% after 5 days, by about 33% after 10 days, and by about 58% after 15 days, indicating that the nutritional gel tablets release water and potassium ions dissolved in water at a uniform rate. The comparative example 1 is modified without adding sodium lignosulfonate, the comparative example 2 is modified without adding pectin to increase stability, so that the water release rate at the initial stage is higher, the death of seedlings at the initial stage of strong seedling rooting cultivation is easily caused, the comparative example 3 is gel due to the fact that sodium trimetaphosphate is not added, the water release amount is too small, normal water and nutrition cannot be supplied to the seedlings, the comparative example 4 adopts chitosan as a main raw material, and the water supply amount cannot be stably and continuously guaranteed due to the fact that the later stage water loss rate is too high.

(2) The survival rate of the cultured Japanese beautyberry in example 1 is about 92%, compared with the blank group in comparative examples 1-4, the survival rate is greatly increased, the beautyberry seedlings grow normally, the root systems are more, the symptoms of obvious phosphorus and potassium deficiency, such as leaf edge yellowing and focal spots, do not appear, and the gel sheet prepared in example 1 is suitable for culturing the Japanese beautyberry. The gel sheets in comparative example 3 and the blank group showed rotten and mildew spots and rotten root symptoms, which indicates that the gel sheets not prepared by the method disclosed by the invention are extremely easy to rot and mildew to cause seedling death when buried in a substrate.

Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention as defined in the appended claims. The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims

1. A method for rapid and efficient propagation of Japanese beautyberry is characterized by comprising the following steps:

(5) strengthening seedlings outside bottles and rooting integrated culture: cutting strong subculture clumpy buds into single buds with the length of 2-3cm, performing out-bottle strong seedling and rooting integrated culture, and continuously transferring the bottom of the single buds into subculture, wherein the specific integrated culture method comprises the following steps:

uniformly mixing sterile fine coconut bricks, perlite and fine river sand according to the volume ratio of 3:1:0.5 to obtain a matrix, putting the matrix and a gel nutrition tablet into a hole tray, watering by adopting 1 ‰ carbendazim until the matrix is kneaded into a mass but does not drip, respectively transferring into the hole tray after a single bud cut to be cut is micro-dried, inserting the matrix into a forceps for 1cm, then thoroughly watering by using a rooting liquid, and fully covering by using a transparent film; in the first 5 days, when the film is full of water drops, uncovering the film for ventilation, shaking off the water drops on the film, uncovering the film for 30min every day after 5 days, uncovering the film for 1h respectively in the morning and afternoon after 10 days, after the length of a new root growing is 0.5cm, completely uncovering the film in the daytime, covering the film at night, spraying 1% compound fertilizer after new leaves grow out, and normally maintaining after completely uncovering the film;

the gel nutrition tablet comprises the following raw materials: 30-50 parts of Shuo' e starch, 10-15 parts of sodium lignosulphonate, 15-18 parts of pectin, 5-10 parts of sodium trimetaphosphate and 8-12 parts of potassium dihydrogen phosphate;

the preparation method of the gel nutrition tablet comprises the following steps:

2. The method for rapid and efficient propagation of Japanese beautyberry according to claim 1, wherein before the explant is picked, 0.1wt% of thiophanate methyl is sprayed in the morning and evening of a sunny day for more than 3 consecutive days in the early winter, late spring, and after 3 days of spraying, the tender bud of Japanese beautyberry which is not developed by leaves is picked as the explant by avoiding burning sun and dew.

3. The method for the rapid and efficient propagation of Japanese beautyberry according to claim 2, wherein in the aseptic treatment of the explant, the surface dust of the explant is washed clean by running water, then washed by 1 ‰ soap water for 1min by shaking, washed by running water for 15min, transferred to a clean bench for 3 times by using sterile water, and after the water is filtered out by sterile filter paper, transferred to 1 ‰ mercuric chloride Tween disinfectant for shaking and disinfection for 6min, and then rinsed three times by sterile water, and the water is filtered out.

4. The method for rapid and efficient propagation of Japanese beautyberry according to claim 3, wherein the formula of the primary culture medium is as follows: MS +6-BA 2.5mg/L + NAA 0.2mg/L + agar 6mg/L + white sugar 30 g/L.

5. The method for rapid and efficient propagation of Japanese beautyberry according to claim 4, wherein the formula of the subculture medium is as follows: MS +6-BA 1.5mg/L + NAA 0.1mg/L + vitamin H1.5mg/L + agar 6mg/L + white sugar 30 g/L.

6. The method for rapid and efficient propagation of callicarpa japonica according to claim 5, wherein the formula of the rooting solution is: 1/2MS + IBA 0.5mg/L + NAA 0.1 mg/L.

7. The method for rapid and efficient propagation of callicarpa japonica according to claim 6, wherein the illumination intensity, the illumination time and the culture temperature are all 1800Lux, 12h/d and 23-27 ℃ respectively in the primary culture, the secondary culture, the out-of-bottle strong seedling and the rooting integrated culture.

8. The method according to claim 7, wherein the substrate is placed in the aperture disk, the gel nutrition tablet is laid on the aperture disk before a part of the substrate is placed in the aperture disk, and then a layer of the substrate with a thickness of 1-2cm is laid on the gel nutrition tablet.