CN117581875B - Composite plant growth regulator and application thereof - Google Patents

Composite plant growth regulator and application thereof Download PDF

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Publication number
CN117581875B
CN117581875B CN202410081521.8A CN202410081521A CN117581875B CN 117581875 B CN117581875 B CN 117581875B CN 202410081521 A CN202410081521 A CN 202410081521A CN 117581875 B CN117581875 B CN 117581875B
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China
Prior art keywords
plant growth
growth regulator
rooting
application
cutting
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CN117581875A (en
Inventor
林金星
胡洪飞
张贵芳
张曦
李晓娟
张莹莹
张妍
李琴
陈亚立
付凯
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Nanchang Innovation Research Institute Of Peking University
Beijing Forestry University
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Nanchang Innovation Research Institute Of Peking University
Beijing Forestry University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention provides a composite plant growth regulator and application thereof. The compound plant growth regulator comprises active ingredients, wherein the active ingredients comprise methyl anthranilate and indolebutyric acid, and the mass ratio of the methyl anthranilate to the indolebutyric acid is 4.5-153:200-400. The invention effectively shortens the rooting time of plants during planting, transplanting or cutting by utilizing the synergistic effect of the two components, and greatly increases the rooting quantity and quality.

Description

Composite plant growth regulator and application thereof
Technical Field
The application relates to the field of plant growth regulators, in particular to a composite plant growth regulator and application thereof.
Background
Rooting is an important stage of plant growth, and it marks that plants start to establish their root systems in new environments, absorb moisture and nutrients, and stabilize their bodies. Healthy root systems are critical to plant growth, and can help plants maintain balanced moisture absorption, stabilize plant posture, and provide nutrients for plants to perform photosynthesis and metabolic activities. Therefore, rapid rooting is critical to the planting, transplanting and cutting of plants.
At present, products for promoting rooting in the market mainly comprise auxins with single components and analogues thereof, and the rooting effect on cutting of many plants is not obvious.
For this purpose, the present invention is proposed.
Disclosure of Invention
The invention mainly aims to provide a composite plant growth regulator and application thereof, and the regulator utilizes the synergistic effect of two components to effectively shorten the rooting time during plant planting, transplanting or cutting, and greatly increase the rooting quantity and quality.
In order to achieve the above object, the present invention provides the following technical solutions.
The first aspect of the invention provides a compound plant growth regulator, which comprises active ingredients, wherein the active ingredients comprise methyl anthranilate and indolebutyric acid, and the mass ratio of the methyl anthranilate to the indolebutyric acid is 4.5-153:200-400, preferably 4.535-152.26:200-400.
Therefore, the invention takes the methyl anthranilate and the indolebutyric acid as the effective components, and can achieve the following effects: the addition of the methyl anthranilate can effectively promote the synthesis of indoleacetic acid in plants, and the indoleacetic acid and the added indolebutyric acid act together, so that the content of auxin in cuttings can be rapidly increased, the formation of adventitious roots is promoted, the rooting time is shortened, and the rooting quantity and the rooting length are effectively increased. In a word, the invention utilizes the synergy of the methyl anthranilate and the indolebutyric acid to play a role of 1+1> 2.
The composite plant growth regulator of the invention can only contain active ingredients, and can also form a stable formulation with auxiliary agents.
Further, the adhesive further comprises an auxiliary agent, wherein the auxiliary agent comprises at least one of a binder, a filler, a solvent and a stabilizer.
The auxiliary agents are favorable for preparing the active ingredients into a preparation form with good stability and favorable absorption by plants.
The binder may be one or more of clay, bentonite, montmorillonite, attapulgite and kaolin, gypsum, cellulose, titanium dioxide, zinc oxide, starch, dextrin, sugar (e.g. lactose, sucrose), silica, talc, mica, diatomaceous earth, urea, etc. These binders help to stably bind the active ingredient to the root/basal surface of the plant, and to prolong the contact time, thereby improving the efficacy.
The solvent can be a component which can uniformly disperse the active ingredient and has little or no damage to the plant rhizome.
Further, the binder includes clay.
Further, the solvent includes water.
Further, the composite plant growth regulator is powder or water aqua.
Further, in the aqueous solution, the concentration of the methyl anthranilate is 30 mu M-1mM, and the concentration of the indolebutyric acid is 200-400mg/L.
Further, the concentration of the methyl anthranilate is 50 mu M-1mM, and the synergy is more obvious.
A second aspect of the present invention provides the use of a composite plant growth regulator of the first aspect for cutting of plants.
The application is not limited only to the application of the composite plant growth regulator of the invention, but also can be applied to direct planting, transplanting and the like of plants.
Further, the compound plant growth regulator is used for cutting of the boxwood and the glossy privet.
Further, the method comprises the steps of: soaking the base of the plant spike with a composite plant growth regulator.
The soaking time and temperature can be adjusted according to actual requirements. In practical application, if the composite plant growth regulator is powder, the effective components can be dissolved by a small amount of solvents such as ethanol, and then diluted to the required concentration by a large amount of water after mixing, and then used for soaking plants.
In conclusion, compared with the prior art, the invention achieves the following technical effects:
The plant growth regulator taking the methyl anthranilate and the indolebutyric acid as the effective components can effectively promote the synthesis of indolebutyric acid in plants, and the plant growth regulator can be combined with the indolebutyric acid to promote the content of auxin in cuttings rapidly, promote the formation of adventitious roots, shorten the rooting time, and effectively increase the rooting quantity and the rooting length.
The foregoing description is only an overview of the present application, and is intended to be implemented in accordance with the teachings of the present application in order that the same may be more clearly understood and to make the same and other objects, features and advantages of the present application more readily apparent.
Drawings
Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the application. Also, like reference numerals are used to designate like parts throughout the accompanying drawings. In the drawings:
FIG. 1 is a map of a breeding base on which the test of the present invention is based;
FIG. 2 is a diagram of a test cutting process according to the present invention;
FIG. 3 shows rooting results of the control group treated with the Buxus japonica according to the embodiments of the present invention;
FIG. 4 shows rooting results after treatment of ligustrum japonicum according to various embodiments of the present invention and control groups.
Detailed Description
Embodiments of the technical scheme of the present application will be described in detail below with reference to the accompanying drawings. The following examples are only for more clearly illustrating the technical aspects of the present application, and thus are merely examples, and are not intended to limit the scope of the present application.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "comprising" and "having" and any variations thereof in the description of the application and the claims and the description of the drawings above are intended to cover a non-exclusive inclusion.
In the description of the embodiments of the present application, the meaning of "plurality" is two or more unless explicitly defined otherwise.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment may be included in at least one embodiment of the application. The appearances of such phrases in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Those of skill in the art will explicitly and implicitly appreciate that the embodiments described herein may be combined with other embodiments.
Unless otherwise specified in the examples and the control group below, "A" means methyl anthranilate and "B" means indolebutyric acid.
Example 1
Firstly, weighing 30 mu mol of the finite component A by using a pipette, dissolving by using 10ml of absolute ethyl alcohol after weighing, and standing for later use; and weighing 0.3g of the limited component B, dissolving the limited component B with 10ml of absolute ethyl alcohol after weighing, standing for standby, mixing the two, and adding water to dilute to 1L.
Example 2
Firstly, weighing 50 mu mol of the finite component A by using a pipette, dissolving by using 10ml of absolute ethyl alcohol after weighing, and standing for later use; and weighing 0.3g of the limited component B, dissolving the limited component B with 10ml of absolute ethyl alcohol after weighing, standing for standby, mixing the two, and adding water to dilute to 1L.
Example 3
Firstly, weighing 1mmol of the limited component A by using a pipette, dissolving the weighed limited component A by using 10ml of absolute ethyl alcohol, and standing for later use; and weighing 0.3g of the limited component B, dissolving the limited component B with 10ml of absolute ethyl alcohol after weighing, standing for standby, mixing the two, and adding water to dilute to 1L.
Control group 1
And (5) clean water.
Control group 2
Rooting powder of trypan (50%) is diluted by 800 times of liquid, 1.2g is weighed, and water is added for dilution to 1L.
Control group 3
Indolebutyric acid 300mg/L: weigh 0.3g in a beaker and dissolve with 5ml absolute ethanol and dilute to 1L with water.
Control group 4
Firstly, weighing 5 mu mol of the finite component A by using a pipette, dissolving by using 10ml of absolute ethyl alcohol after weighing, and standing for later use; and weighing 0.3g of the limited component B, dissolving the limited component B with 10ml of absolute ethyl alcohol after weighing, standing for standby, mixing the two, and adding water to dilute to 1L.
Control group 5
Firstly, weighing the limited component A2 mmol by a pipette, dissolving the weighed limited component A2 mmol by 10ml of absolute ethyl alcohol, and standing for later use; and weighing 0.3g of the limited component B, dissolving the limited component B with 10ml of absolute ethyl alcohol after weighing, standing for standby, mixing the two, and adding water to dilute to 1L.
The above examples and control groups were used for cutting of boxwood melon seeds and ligustrum japonicum, as follows.
Buxus sinica (Rehder & E.H. Wilson) M.Cheng: the plant is a plant which is cultivated in a large quantity in a long triangular area and is used for planting a plurality of plants which are planted in a field, is commonly used for planting basic plants of color blocks and color strips, can be planted in flower beds, tree beds and garden intersections, and has good ornamental effect.
Ligustrum japonicum (Latin brand name: ligustrum japonicum 'Howardii'): is a small arbor of ligustrum of Oleaceae, the leaf color of the ligustrum japonicum is golden, the plant shape is beautiful, and the plant is an excellent hedge tree species, the growth vigor is strong and the germination capacity is strong; the bottom branches and the inner branches are not easy to wither, and have strong resistance to diseases and insect pests, fire, soot, wind and snow and the like; in addition, the blade texture is good, the plant shape is compact, and the natural hedge material is very good. Meanwhile, the ligustrum japonicum has strong disease resistance, can absorb a large amount of dust and harmful gas in the air, and can purify the air.
The existing propagation modes of the two species mainly adopt cutting propagation, but the traditional cutting rooting time is too long, the rooting quantity is not large, the time of out-nursery sales is increased to a certain extent, in order to help tree species to accelerate the rooting time and shorten the out-nursery time, the invention specially selects the test tree species, and aims to accelerate the turnover rate, realize the large-scale process and realize the same land income-creating turnover.
The test steps are as follows:
1, spike selection: 1-2 years old female tree branches, the current year half lignified scions (3-4 cm), preferably top scions, remain side branches and terminal buds, and are harvested and transplanted on the same day.
2, Test base: loess (containing sand) of a breeding base of Nanchang, according to 1.2m wide ridge (as shown in figure 1), turning over soil and leveling 3 days in advance, treating the substrate with 1000 times of potassium permanganate for sterilization and disinfection and filling water in the furrows before cutting, and guaranteeing the substrate to be moist.
3. And (3) a growth agent: the products of the previous examples and control groups were selected.
4. The specific treatment comprises the following steps:
cutting the spike with half lignification degree of 3-4cm (120 branches of each experimental group), and soaking the base of the spike in the growth agent at the 3 rd point for about 2cm for 2 hours.
Cutting for 6-7 months, and cutting with soil depth of 2-3cm, cutting with wood stick, spraying carbendazim, and coating with film (as in cutting process of figure 2).
5. Evaluation of results:
The data statistics of this time is: rooting conditions were cut for one month (30 days), and the data are shown in fig. 3 and 4 below.
Average adventitious root number = number of adventitious roots/total measured cutting shoot number
Average length of adventitious roots = length of each adventitious root/total measured adventitious root number
Survival rate = (number of survival branches/total number of cutting branches) ×100%
Rooting rate= (number of rooting branches/total number of cutting branches) ×100%
As can be seen from the statistics of fig. 3 and 4: the average number of adventitious roots of the littleleaf boxwood in the control group 1, the control group 2 and the control group 3 is 4.65,6 and 12.5 respectively, and the average lengths of the adventitious roots are 3.4cm,6.5cm and 7 cm respectively; the average number of adventitious roots for examples 1 to 3 was 15.75, 16.25 and 18.75, respectively, and the average lengths were 9.1cm, 9.8 cm and 8cm, respectively. The above results indicate that the time for the example cuttings to begin to root, the number and length of adventitious roots produced are significantly better than the control. And the effect of example 3 is more superior to examples 1 and 2.
In the test of the ligustrum japonicum, the cuttings treated by the control group 1, the control group 2 and the control group 3 generate certain adventitious roots, the average number of the adventitious roots of the ligustrum japonicum is 4.2,6.8 and 8.1 respectively, and the average lengths of the adventitious roots are 4.5 cm,5.2cm and 6 cm respectively; the average number of adventitious roots for examples 1 to 3 was 8.1, 8.5 and 9.7, respectively, and the average lengths were 7.2cm, 7.3 cm and 8.6 cm, respectively. The above results indicate that the time for the example cuttings to begin to root, the number and length of adventitious roots produced are significantly better than the control. And the effect of example 3 is more superior to examples 1 and 2.
Thus: the invention not only greatly improves the rooting speed, but also greatly improves the rooting quantity, and can obviously show that the effect is far better than that of similar rooting products and a clear water treatment control group.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present application, and not for limiting the same; although the application has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the application, and are intended to be included within the scope of the appended claims and description. In particular, the technical features mentioned in the respective embodiments may be combined in any manner as long as there is no structural conflict. The present application is not limited to the specific embodiments disclosed herein, but encompasses all technical solutions falling within the scope of the claims.

Claims (6)

1. The compound plant growth regulator is characterized by comprising an active ingredient, wherein the active ingredient consists of methyl anthranilate and indolebutyric acid; the compound plant growth regulator is water aqua, the concentration of the methyl anthranilate is 30 mu M-1mM, and the concentration of the indolebutyric acid is 200-400mg/L.
2. The composite plant growth regulator of claim 1, further comprising an auxiliary agent comprising at least one of a binder, a filler, and a stabilizer.
3. A composite plant growth regulator according to claim 2, wherein the binder comprises clay.
4. A composite plant growth regulator according to any one of claims 1-3, wherein the concentration of methyl anthranilate is 50 μm-1mM.
5. The use of the compound plant growth regulator according to any one of claims 1 to 4, wherein the compound plant growth regulator is used for cutting of boxwood and glossy privet fruit.
6. The use of a composite plant growth regulator according to claim 5, comprising: soaking the base of the plant spike with a composite plant growth regulator.
CN202410081521.8A 2024-01-19 2024-01-19 Composite plant growth regulator and application thereof Active CN117581875B (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1746160A (en) * 2004-09-06 2006-03-15 天津师范大学 Synthesis of plant growth hormone 3-indolebutyric acid
CN102438456A (en) * 2009-04-07 2012-05-02 塔明克股份有限公司 Pollination improver
CN102438453A (en) * 2009-04-07 2012-05-02 塔明克股份有限公司 Plant growth regulator additive
WO2016035685A1 (en) * 2014-09-01 2016-03-10 雪印種苗株式会社 Adventitious root formation inducer and root system growth promoter
CN109601547A (en) * 2018-11-30 2019-04-12 北京市园林科学研究院 A kind of plant pollen removes medicament and plant pollen removes method
JP2023164356A (en) * 2022-04-28 2023-11-10 雪印種苗株式会社 Inhibitor for activity of auxin and anthranilic acid

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US20120101164A1 (en) * 2009-04-07 2012-04-26 Taminco, Naamloze Vennootschap Abscission and crop storage unit

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CN1746160A (en) * 2004-09-06 2006-03-15 天津师范大学 Synthesis of plant growth hormone 3-indolebutyric acid
CN102438456A (en) * 2009-04-07 2012-05-02 塔明克股份有限公司 Pollination improver
CN102438453A (en) * 2009-04-07 2012-05-02 塔明克股份有限公司 Plant growth regulator additive
WO2016035685A1 (en) * 2014-09-01 2016-03-10 雪印種苗株式会社 Adventitious root formation inducer and root system growth promoter
CN109601547A (en) * 2018-11-30 2019-04-12 北京市园林科学研究院 A kind of plant pollen removes medicament and plant pollen removes method
JP2023164356A (en) * 2022-04-28 2023-11-10 雪印種苗株式会社 Inhibitor for activity of auxin and anthranilic acid

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