CN103688738A - Cutting propagation method for fiveleaf akebia fruits - Google Patents
Cutting propagation method for fiveleaf akebia fruits Download PDFInfo
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Abstract
The invention discloses a cutting propagation method for fiveleaf akebia fruits, and belongs to the field of plant cultivation. The cutting propagation method for the fiveleaf akebia fruits comprises the following steps of shearing annual fiveleaf akebia fruit branches into cutting slips with certain lengths; soaking the cutting slips in somatropin solution to obtain soaked cutting slips; and inserting the soaked cutting slips in a matrix. After cutting, the cutting slips are sprayed with water sufficiently, a tunnel is constructed, films cover the cutting slips, light transmittance is kept in a range of 50%-80%, the relative humidity is 80%-90%, the temperature is controlled in a range of 25 DEG C to 30 DEG C, and the films can be removed after roots are fixed. By using the cutting propagation method for the fiveleaf akebia fruits, the problems that differentiation and genetic diversity of populations in the traditional seed propagation method are high, and the merit of female parents is difficult to keep are solved; and the cutting propagation method is simple and feasible, and fills in a gap of cutting propagation culture on fiveleaf akebia fruits which are wild plant resources. By using the cutting propagation method, seedlings of the fiveleaf akebia fruits can be obtained quickly; and a favorable provenance guarantee is provided for effectively developing the medical value of the fiveleaf akebia fruits.
Description
Technical field
The present invention relates to the cuttage breeding method of plant, relate in particular to a kind of YANGKAIKOU cuttage breeding method.
Background technology
At present the commodity of YANGKAIKOU medicinal material are mainly derived from the root of exhibition hair tomonea (Melastoma normale D.Don) and congener tomonea (M1candidum D.Don), also as YANGKAIKOU, use at part producing region congener manyflower melastoma herb (M1affine D.Don) and the root of mao harvesting (M1sanguineum Sims) simultaneously.Slightly warm in nature, taste is sweet, sour, puckery, has stopping diarrhea with astringents, astringing to arrest bleeding, regulate the flow of vital energy relax liver, promoting diuresis for stranguria, the effect such as anticancer relieves internal heat, being mainly used in the diseases such as treatment is let out under dysentery, uterine bleeding band, interior traumatism and bleeding, is conventional Chinese medicine among the people, is also one of main flavour of a drug of prescribed preparation three gold plaques.
At present, the main source of YANGKAIKOU is purchase natural crude drugs, and still, because people is excessive and random lumbering, resource approaches exhausted, therefore develops YANGKAIKOU artificial planting, significant to expanding population scale.The Sterile culture method of YANGKAIKOU be take seminal propagation as main, and the method has the differentiation of population and genetic diversity is large, the problem that is difficult to keep maternal merit.
Chinese scholars so far to the research direction of YANGKAIKOU medicinal material mainly aspect pharmacognostic evaluation, Medicinal Materials Characters, chemical composition, pharmacological action, clinical four, YANGKAIKOU is also bred and is not related to.In order to keep its merit, carry out particularly Technique on Cuttage Propagation research of vegetative propagation, be not only and accelerate YANGKAIKOU cuttage sapling multiplication, also significant for preservation, development and the utilization of YANGKAIKOU resource.It is feasible adopting cuttings to carry out extensive booth cuttage and seedling culture.
Summary of the invention
The technical problem to be solved in the present invention is to overcome the deficiency that the conventional seed propagation method of YANGKAIKOU is difficult to keep maternal merit, provide a kind of and can keep the maternal good genetic character of YANGKAIKOU, and short, simple cuttage breeding method of seed selection time is to realize the fast-propagation of YANGKAIKOU.
For solving the problems of the technologies described above, the present invention adopts technical scheme to be:
The cuttage breeding method that the present invention relates to a kind of YANGKAIKOU, comprises the following steps:
1) annual YANGKAIKOU branch is cut into the cuttings of certain length;
2) cuttings is put into hormone solution and soaked, obtain the cuttings through soaking;
3) cuttings through soaking is inserted in matrix.
In step 1), described YANGKAIKOU branch is to gather the early flowering season; The length of described cuttings is 8～12cm; Described cuttings is lignification branch, semi-lignified branch or spray, preferably spray; And preferred major branch top and major branch bottom, more preferably major branch top.
In step 2) in, described hormone solution is a kind of in heteroauxin and the methyl α-naphthyl acetate mol ratio mixture solution that is 1:1, indolebutyric acid solution, naphthalene acid solution, preferably indolebutyric acid solution; The concentration of described hormone solution is 0.05～0.5mmol/L, preferably 0.05～0.2mmol/L, more preferably 0.1mmol/L.
In step 2) in, soak time is 6～24 hours, preferably 12～24 hours, and more preferably 18 hours.
In step 3), by 8cm * 8cm～10cm * 10cm seeding row spacing, insert in matrix with horizontal plane is angled, cuttage angle is 30～60 degree, preferably 45 degree; The cuttage degree of depth is 2/3～3/4 of cutting length.
Matrix described in step 3) is selected from a kind of in river sand, peat soil, perlite, or is selected from mixture that peat soil and perlite volume ratio are 1:2 or the volume ratio of garden mould and fire soil is 4:1 mixture, preferably river sand.
Preferably, the cuttage breeding method of YANGKAIKOU, also comprises the management method after cuttage, comprises watering after cuttage permeablely, takes shed lid film, ventilate, water spray, and controlled light, temperature and humidity; Keeping light transmittance is 50%～80%, preferably 60%, and relative moisture is 80%～90%, and temperature is 25～30 ℃, and normal root recession thin film obtains the cuttings of normal root after 2 months.
Step 3) carries out disinfection to cutting medium before also comprising cuttage, and sterilization adopts the carbendazim solution that concentration is 0.05wt%～0.3wt%, preferably 0.1wt%.
The rooting rate of the cuttings that the cuttage breeding method of described YANGKAIKOU obtains is 50%～59.4%.
In the cuttage breeding method of described YANGKAIKOU, the number of taking root is 10～57, and root length is 5.1～14.2cm.
In the booth that is experimental field located at Guangxi Plant Inst.'s introduction and cultivation base of the present invention, during cuttage, to note the daytime according to actual conditions are sufficient at sunshine, temperature is higher, suitable opens slotting machine sprayer unit and closes shade net, the suitable temperature and humidity environment needing to maintain rooting of cuttings; At night, all the time according to, time period that temperature is lower, close sprayer unit and open shade net.Should guarantee slotting machine humidity, avoid again cuttings because crossing wet rotting of causing, also be unfavorable for that cuttings forms callus.Cuttage in spring YANGKAIKOU, forms callus in approximately 7～15 days, and approximately 14～25 days start to take root.
Adopt after technique scheme, the present invention compared with prior art has following beneficial effect.
The invention solves the problem that the differentiation of population in current prior seed propagation method and genetic diversity are large, be difficult to keep maternal merit, simple, filled up the cottage propagation of the wild plant resource YANGKAIKOU blank of growing seedlings, the method can effectively realize fast fetching and obtain YANGKAIKOU seedling, for its pharmaceutical value of effective exploitation provides favourable provenance guarantee.
Embodiment
Embodiment 1
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch top of lignification branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, cuttings is put into the heteroauxin of 0.1mmol/L, in methyl α-naphthyl acetate 1:1 mixture solution, soak 6h, press 9cm * 9cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 55, and average root is long is 12.3cm, and rooting rate is 58.3%.
Embodiment 2
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch top of semi-lignified branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the indolebutyric acid solution of cuttings being put into 0.2mmol/L soaks 6h, press 8cm * 8cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 52, and average root is long is 14.1cm, and rooting rate is 54.6%.
Embodiment 3
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is spray major branch bottom, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the naphthalene acid solution of cuttings being put into 0.5mmol/L soaks 6h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 44, and average root is long is 13.6cm, and rooting rate is 58.1%.
Embodiment 4
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch bottom of lignification branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the indolebutyric acid solution of cuttings being put into 0.5mmol/L soaks 12h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 49, and average root is long is 11.6cm, and rooting rate is 55.1%.
Embodiment 5
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch middle part of semi-lignified branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the naphthalene acid solution of cuttings being put into 0.1mmol/L soaks 12h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 57, and average root is long is 14.2cm, and rooting rate is 55.6%.
Embodiment 6
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Select the major branch top that cuttage branch is spray, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, cuttings is put into the heteroauxin of 0.2mmol/L, in methyl α-naphthyl acetate 1:1 mixture solution, soak 12h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 45, and average root is long is 12.6cm, and rooting rate is 54.7%.
Embodiment 7
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is lignified branch major branch middle part, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the naphthalene acid solution of cuttings being put into 0.2mmol/L soaks 18h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 42, and average root is long is 11.8cm, and rooting rate is 54.4%.
Embodiment 8
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch top of semi-lignified branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, cuttings is put into the heteroauxin of 0.5mmol/L, in methyl α-naphthyl acetate 1:1 mixture solution, soak 18h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 37, and average root is long is 13.8cm, and rooting rate is 56.2%.
Embodiment 9
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Cuttage is watered peat soil matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Select the major branch top that cuttage branch is spray, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the indolebutyric acid solution of cuttings being put into 0.1mmol/L soaks 18h, press 10cm * 10cm seeding row spacing oblique cutting in dark peat soil matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 54, and average root is long is 12.9cm, and rooting rate is 51.2%.
Embodiment 10
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with 20cm river sand matrix in long 30m, wide 1.2m, slotting machine.Cuttage is watered river sand matrix in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Select the major branch bottom that cuttage branch is spray, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, cuttings is put into the heteroauxin of 0.2mmol/L, in methyl α-naphthyl acetate 1:1 mixture solution, soak 6h, press 10cm * 10cm seeding row spacing oblique cutting in river sand matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 54, and average root is long is 14.0cm, and rooting rate is 51.2%.
Embodiment 11
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, in long 30m, wide 1.2m, slotting machine, is covered with 20cm pearlite interstitial substance.Cuttage is watered pearlite interstitial substance in first 2～3 days permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch bottom of lignification branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the naphthalene acid solution of cuttings being put into 0.1mmol/L soaks 18h, press 10cm * 10cm seeding row spacing oblique cutting in pearlite interstitial substance, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 45, and average root is long is 12.9cm, and rooting rate is 55.7%.
Embodiment 12
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the matrix that 20cm peat soil and perlite volume ratio are 1:2 in long 30m, wide 1.2m, slotting machine.The matrix that first 2～3 days of cuttage is 1:2 by mud peat soil and perlite volume ratio is watered permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Selecting cuttage branch is the major branch top of semi-lignified branch, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the indolebutyric acid solution of cuttings being put into 0.2mmol/L soaks 12h, press 10cm * 10cm seeding row spacing oblique cutting in peat soil: in perlite=1:2 matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 37, and average root is long is 13.9cm, and rooting rate is 50.8%.
Embodiment 13
Material is got the plant of in March, 2011 introducing and planting Guangxi Plant Inst. proving ground, gather life in 1 year on plant, robust growth on April 20th, 2012 (early flowering season), without the YANGKAIKOU branch of damage by disease and insect as cuttage cuttings.The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the matrix that 20cm garden mould and fiery native volume ratio are 4:1 in long 30m, wide 1.2m, slotting machine.The matrix that first 2～3 days of cuttage is 4:1 by garden mould and fiery native volume ratio is watered permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
Select the major branch top that cuttage branch is spray, spike length is the 50 strain cuttings of 8～12cm, cuttings upper cut straight snips, lower cut is tiltedly cut, clip is level and smooth, the naphthalene acid solution of cuttings being put into 0.5mmol/L soaks 18h, press 10cm * 10cm seeding row spacing oblique cutting in yellow soil: in fiery soil=1:1 matrix, approximately 45 °, angle of inclination, the cuttage degree of depth is 2/3 of cutting length, after cuttage, water permeable, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity, keep light transmittance 60%, relative moisture is 80%～90%, temperature is controlled at 25～30 ℃, after normal root, can remove film.After two months, the number of on average taking root that statistics obtains cuttings is 57, and average root is long is 13.7cm, and rooting rate is 56.2%.
Embodiment in above-described embodiment can further combine or replace; and embodiment is described the preferred embodiments of the present invention; not the spirit and scope of the present invention are limited; do not departing under the prerequisite of design philosophy of the present invention; the various changes and modifications that in this area, professional and technical personnel makes technical scheme of the present invention, all belong to protection scope of the present invention.
Test example 1: orthogonal experiment
1. test period and place
Experimental field be located in the booth in Guangxi Plant Inst.'s introduction and cultivation base base height above sea level 170m, 19.3 ℃ of year-round average temperatures, within the hottest month, (July) mean temperature is 28 ℃, and within the coldest month, (January) mean temperature is 8.4 ℃, 38 ℃ of the extreme highest temperatures, extreme lowest temperature-6 ℃, there is the accidental snow of frost in the winter.Annual rainfall 1655.6mm, rainfall concentrates on for 4～June, and winter, rainfall was less, and alternation of wetting and drying is obvious, annual relative moisture 78%.
The booth of cottage propagation test, ceiling have mobilizable shade net (shading rate is 75%), can be according to weather condition Push And Release.Slotting machine is east-west, is covered with the dark peat soil matrix of 20cm in long 30m, wide 1.2m, slotting machine.Front 2～the 3d of cuttage waters peat soil permeable, with rake, slotting machine is raked, and with 0.1% carbendazim solution, matrix is carried out disinfection.
2. orthogonal experiment
For studying the impact of different factors on YANGKAIKOU cuttage and seedling culture, select soak time (A), cuttings kind (B), hormone kind (C), 4 factors of hormone concentration (D), each factor is got 3 levels, by L9 (3
4) table carries out orthogonal experiment (table 1).9 treatment combinations, every processing 50 strain cuttings are established in test altogether.
Note: cuttings kind is got lignification branch on annotinous branch, semi-lignified branch, spray (according to the shoot growth situation more leaf that stays of spray blade only, therefore spray stays 2, leaf).Spray is the tender branch of children; Cell wall, because the deposition of the lignin that cell produces becomes hard firm, has increased the ability of plant support gravity, forms lignification branch; Semi-lignified branch is exactly that spray is to the hypertrophy stage of lignification branch.
Cuttings upper cut straight snips, lower cut is tiltedly cut, and clip is level and smooth, and 2, leaf is stayed in spray cutting.Spike length 8～12cm, by 10cm * 10cm seeding row spacing oblique cutting in matrix, approximately 45 °, inclination angle, the cuttage degree of depth is cutting length 2/3.After cuttage, water permeablely, take shed lid film, and note ventilating, water spray, controlled light, temperature and humidity; Keep light transmittance 60%, relative moisture is 80%～90%, and temperature is controlled at 25～30 ℃.After normal root, can remove film.
After cuttage, within every 5 days, randomly draw the callus of cuttings observational record cuttings and the formational situation of taking root.After 2 months to the rooting rate of each processing, on average take root number, average root progress row statistics, and take rooting rate, mean elements, long 3 variablees of average root and Rooting index carried out to overall merit as index.Wherein, rooting rate refers to that the cuttings of taking root in the same group of cuttings of processing accounts for the ratio that this organizes all cuttings.Number and the average root length of on average taking root refers to that taking root of the interior average every strain cuttage seeding of each group counted and root is long.
Rooting index=rooting rate * mean elements * average root is long;
The statistical analysis of data is with carrying out range analysis in SAS8.1 software.SPSS18.0 software is made one-way analysis of variance (one-way ANOVA), by Duncan method, carries out multiple ratio, heterogeneity of variance, adopt Tamhane ' s T2 to compare.
Table 1 L9 (3
4) experimental factor and level design table
YANGKAIKOU, after cuttage in spring, starts Lao Ye for 3 days and turns yellow, within 5 days, starts fallen leaves, within 7～15 days, forms callus, and 14～25 days start to take root.In the experiment quadrature cuttage to YANGKAIKOU in the 60th day test, measure.Orthogonal experiments is in Table 2.
Table 2 orthogonal experiments
The reflection of table 3 range analysis the primary and secondary of each factor on result of the test impact.As can be seen from Table 3, the primary and secondary pass that each factor affects effect to rooting percent is: B>D>C>A, illustrate that cuttage cuttings has the greatest impact to the rooting rate of YANGKAIKOU cottage propagation, other is followed successively by hormone concentration, hormone kind, soak time, with A
1b
3c
2d
1best of breed for cuttage root-taking rate.
The range analysis of each experimental factor of table 3 on rooting rate impact
Because the sum of squares of deviations of A factor is minimum, with this because usually making error term, carry out variance analysis, the results of analysis of variance shows that cuttings reaches utmost point significance level (p<0.01) to the impact of rooting rate, all the other factors reach significance level (p<0.05) to the impact of rooting rate, and result is as shown in table 4.
The variance analysis of each experimental factor of table 4 on rooting rate impact
Note: p<0.01 is that utmost point significance level represents with " * * ", and 0.01<p<0.05 significance level represents with " * ".
2.1 different factors are on YANGKAIKOU cuttage several impact of on average taking root
Range analysis result from each experimental factor of table 5 on the number of on average taking root, the long impact of average root, the size sequence that each factor affects effect to the number of on average taking root is: A>B>C>D, illustrate that solution soak time has the greatest impact to the number of taking root of YANGKAIKOU cottage propagation, other is followed successively by cuttage cuttings, hormone kind, hormone concentration, with A
3b
3c
2d
1for cuttage several best of breed of on average taking root.
The range analysis of each experimental factor of table 5 on the number impact of on average taking root
Because the sum of squares of deviations of D factor is minimum, with this, because usually making error term, carry out variance analysis, as table 6.Result shows, solution soak time, cuttage cuttings, hormone kind, hormone concentration are on the several impact all not significantly (p>0.05) of on average taking root.
The variance analysis of each experimental factor of table 6 on number (bar) impact of on average taking root
The impact that 2.2 different factors are long on YANGKAIKOU cuttage average root
As shown in Table 7, each factor to the size sequence of the effect length effect of on average taking root is: B>A>D>C, the take root effect length maximum of cuttage branch to YANGKAIKOU cottage propagation is described, other are followed successively by solution soak time, hormone concentration, hormone kind, with A
3b
3c
1d
2for on average the take root best of breed of length of cuttage.
The range analysis of each experimental factor of table 7 on the long impact of average root
Because the sum of squares of deviations of C factor is minimum, with this because usually making error term, carry out variance analysis, result shows, solution soak time, cuttage cuttings, hormone kind are on the impact of the length of on average taking root significantly (0.05>p>0.01).Hormone concentration is on the impact of the length of on average taking root not significantly (p>0.05), and experimental result is as shown in table 8.
The variance analysis of each experimental factor of table 8 on average root long (cm) impact
2.3 impacts of different factors on YANGKAIKOU cuttage root-taking index
Range analysis result from each experimental factor of table 9 on Rooting index impact, the size sequence that each factor affects effect to the number of on average taking root is: B>A>C>D, illustrate that cuttage cuttings has the greatest impact to the Rooting index of YANGKAIKOU cottage propagation, other are followed successively by solution soak time, hormone kind, hormone concentration, with A
3b
3c
2d
1best of breed for cuttage root-taking index.
The range analysis of each experimental factor of table 9 on Rooting index impact
Because the sum of squares of deviations of D factor is minimum, with this, because usually making error term, carry out variance analysis, as table 10.Result shows: solution soak time, cuttage cuttings, hormone kind, hormone concentration are not significance level (p>0.05) to several impact of on average taking root, and just the relative other factors of cuttage cuttings is maximum on several impact of on average taking root.
The variance analysis of each experimental factor of table 10 on Rooting index impact
Test example 2: dual factors test
The hormone solution that soaks cutting adopts the indolebutyric acid of 100ppm, and soak time is 12h.The dual factors experiment of cuttage material and cutting medium.Cuttage material is annotinous branch, minute major branch bottom (lignification), major branch middle part (lignification) and major branch top (semi-lignified) 3 kinds of cuttings.It is 4:1 mixture five kinds that cutting medium divides mixture, perlite or garden mould and the fiery native volume ratio that river sand, peat soil, peat soil and perlite volume ratio are 1:2.According to two factor randomized complete-block designs, experiment has 15 treatment combinations, as shown in table 11:
Table 11 cuttage material and cutting medium treatment combination
Each matrix components:
River sand pH:5.8 main component is silica, and organic and N, P, K are 0;
Peat soil (Nanning Qiu Hua peat processing factory) pH:6, organic 70%, N, P, K >=4%;
Perlite pH:6,100% pure inorganic cultivation matrix;
Garden mould and fiery native pH:5.8, available phosphorus 40.40mg/kg, effective potassium 57.32mg/kg, iron 219mg/kg, calcium 2111mg/kg, chromium 23.04mg/kg, arsenic 25.3mg/kg, nitrogen 1.53mg/kg.
Fire soil refers to combustible plant and earth mixed-stacking and carries out a kind of soil forming after burn incompletely, and rural area is often used as the soil of growing seedlings in fertilizer or crops.
Table 12 cuttage material and cutting medium treatment combination rooting rate (%)
Note: be above designated as the variance ratio of each cuttage cuttings of the same race, under be designated as each matrix of the same race variance ratio.
Variance comparative result from the dual factors test of table 12: the choosing folding at branch position is > major branch middle part, > major branch bottom, major branch top on the sequence that affects effect of average rooting rate, illustrates that the early flowering season in April is carried out cuttage root-taking rate with major branch top branch the highest; Variance comparative result at different cutting mediums is known: cutting medium is also that best is river sand greatly on the impact of average rooting rate, is secondly peat soil+perlite, is peat soil and perlite again, and the poorest is garden mould and fiery native treatment combination.The rooting rate of explanation cuttage YANGKAIKOU in river sand is best.
Table 13 cuttage material and cutting medium treatment combination Rooting index
Note: be above designated as the variance ratio of each cuttage cuttings of the same race, under be designated as each matrix of the same race variance ratio.
Variance comparative result from the dual factors test of table 13: the selection at branch position is > major branch middle part, > major branch bottom, major branch top on the sequence that affects effect of average Rooting index, illustrates that the early flowering season in April is carried out cuttage root-taking index with major branch top branch the highest; Variance comparative result at different cutting mediums is known: cutting medium is also greatly on the impact of average Rooting index, and its sequence is followed successively by river sand mud > peat soil+perlite > peat soil > perlite > garden mould and fire soil.The rooting rate of explanation cuttage YANGKAIKOU in river sand is best.
Test example 3: experiment of single factor
1. the impact of hormone concentration
Selecting annual semi-lignified branch is cuttage material, indolebutyric acid in 0.05mmol/L, 0.1mmol/L, tri-kinds of concentration of 0.2mmol/L soaks respectively, with clear water, contrast, soak time is 12h, cuttage in the matrix that garden mould and fiery native volume ratio are 4:1, relatively the indolebutyric acid under variable concentrations is for the impact of YANGKAIKOU cuttage root-taking survival rate and Rooting index.
The impact of table 14 soaking concentration on YANGKAIKOU survival rate and Rooting index
As shown in Table 14, lignified YANGKAIKOU branch is in soaking with indole butyric acid cuttage in matrix of the same race after 12 hours of variable concentrations, statistical data after 2 months growth a year and a half.Result shows: in the indolebutyric acid that YANGKAIKOU is 0.1mmol/L in concentration, cuttage survival rate is the highest, and Rooting index is best.
2. the impact of soak time
Selecting annual semi-lignified branch in mid-July, 2013 is cuttage material, at soaking with indole butyric acid 6h, 12h, 18h, the 24h of 0.1mmol/L with do not soak (being 0h) for contrast, utilize perlite for matrix cuttage, the impact of more different soak times on YANGKAIKOU cuttage root-taking survival rate and Rooting index.
The impact of table 15 soak time on YANGKAIKOU survival rate and Rooting index
As shown in Table 15, cuttage in matrix of the same race after the soaking with indole butyric acid different time that the semi-lignified YANGKAIKOU branch of growing a year is 0.1mmol/L in concentration, statistical data after 2 months.Result shows: semi-lignified YANGKAIKOU branch survival rate after 18 hours soak cuttage is the highest, and Rooting index is best.The YANGKAIKOU branch survival rate of immersion 12h and Rooting index are secondly.
Claims (10)
1. a cuttage breeding method for YANGKAIKOU, is characterized in that, comprises the following steps:
1) annual YANGKAIKOU branch is cut into the cuttings of certain length;
2) cuttings is put into hormone solution and soaked, obtain the cuttings through soaking;
3) cuttings through soaking is inserted in matrix.
2. the cuttage breeding method of YANGKAIKOU according to claim 1, is characterized in that, in step 1), described YANGKAIKOU branch is to gather the early flowering season; The length of described cuttings is 8～12cm; Described cuttings is lignification branch, semi-lignified branch or spray, preferably spray; And preferred major branch top and major branch bottom, more preferably major branch top.
3. the cuttage breeding method of YANGKAIKOU according to claim 1, it is characterized in that, in step 2) in, described hormone solution is a kind of in heteroauxin and the methyl α-naphthyl acetate mol ratio mixture solution that is 1:1, indolebutyric acid solution, naphthalene acid solution, preferably indolebutyric acid solution; The concentration of described hormone solution is 0.05～0.5mmol/L, preferably 0.05～0.2mmol/L, more preferably 0.1mmol/L.
4. the cuttage breeding method of YANGKAIKOU according to claim 1, is characterized in that, in step 2) in, soak time is 6～24 hours, preferably 12～24 hours, and more preferably 18 hours.
5. the cuttage breeding method of YANGKAIKOU according to claim 1, is characterized in that, in step 3), by 8cm * 8cm～10cm * 10cm seeding row spacing, inserts in matrix with horizontal plane is angled, and cuttage angle is 30～60 degree, preferably 45 degree; The cuttage degree of depth is 2/3～3/4 of cutting length.
6. the cuttage breeding method of YANGKAIKOU according to claim 1, it is characterized in that, matrix described in step 3) is selected from a kind of in river sand, peat soil, perlite, or be selected from mixture that peat soil and perlite volume ratio are 1:2 or the volume ratio of garden mould and fire soil is 4:1 mixture, preferred river sand.
7. the cuttage breeding method of YANGKAIKOU according to claim 1, is characterized in that, also comprises the management method after cuttage, comprises watering after cuttage permeablely, takes shed lid film, ventilate, water spray, and controlled light, temperature and humidity; Keeping light transmittance is 50%～80%, preferably 60%, and relative moisture is 80%～90%, and temperature is 25～30 ℃, and normal root recession thin film obtains the cuttings of normal root after 2 months.
8. the cuttage breeding method of YANGKAIKOU according to claim 1, is characterized in that, step 3) carries out disinfection to cutting medium before also comprising cuttage, and sterilization adopts the carbendazim solution that concentration is 0.05wt%～0.3wt%, preferably 0.1wt%.
9. according to the cuttage breeding method of the YANGKAIKOU described in the arbitrary claim in claim 1～8, it is characterized in that, the rooting rate of the cuttings that described method obtains is 50%～59.4%.
10. according to the cuttage breeding method of the YANGKAIKOU described in the arbitrary claim in claim 1～8, it is characterized in that, described in the number of taking root be 10～57, root length is 5.1～14.2cm.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106613275A (en) * | 2017-01-06 | 2017-05-10 | 彭正锋 | Tree peony root cutting reproduction method |
| CN107242088A (en) * | 2017-07-18 | 2017-10-13 | 福建农林大学 | A kind of cuttage breeding method of that rattan of the five fingers |
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-
2013
- 2013-12-31 CN CN201310752283.0A patent/CN103688738B/en active Active
Non-Patent Citations (2)
| Title |
|---|
| 宋小军等: "野牡丹采穗圃建立和扦插繁殖技术研究", 《四川林业科技》, vol. 28, no. 4, 31 August 2007 (2007-08-31) * |
| 陆璃等: "角茎野牡丹的生物学特性和种植技术要点", 《热带农业工程》, vol. 36, no. 2, 30 April 2012 (2012-04-30) * |
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Address after: 541004 No.9, South Renmin Road, Lingui District, Guilin City, Guangxi Zhuang Autonomous Region Patentee after: Sanjin Pharmaceutical Co., Ltd., Guilin Address before: 541004 No. 1 Jinxing Road, Guilin, the Guangxi Zhuang Autonomous Region Patentee before: Sanjin Pharmaceutical Co., Ltd., Guilin |