CN111149526A

CN111149526A - Method for improving cuttage rooting of Chinese roses

Info

Publication number: CN111149526A
Application number: CN202010012280.3A
Authority: CN
Inventors: 王长泉; 沈倩雅; 刘金义; 陆俊
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2020-01-07
Filing date: 2020-01-07
Publication date: 2020-05-15

Abstract

The invention discloses a method for improving Chinese rose cutting rooting, which mainly comprises the following steps: (1) preparing a methyl jasmonate mother solution: firstly, preparing 1g/L methyl jasmonate aqueous solution from a methyl jasmonate emulsifier as mother solution; (2) preparing cutting slips: selecting branches without plant diseases and insect pests and with good growth vigor as cutting mother branches to be cut into cutting slips; (3) and (3) cutting treatment: diluting the methyl jasmonate mother liquor prepared in the step (1) into a working solution to soak the base of the cutting shoot; (4) preparing a seedbed: preparing a seedling raising plate as a cutting seedbed, and raising seedlings by adopting a matrix cultivation method; (5) tender branch cuttage: immediately cutting the cutting slips processed in the step (3) in a seedling bed of a seedling raising tray; (6) cuttage management: cutting seedling in artificial climate box, setting illumination period as long day and light intensity as weak light intensity, and covering to prevent rapid evaporation of water; and during the cutting seedling raising period, the substrate in the plug is kept moist but water cannot be accumulated.

Description

Method for improving cuttage rooting of Chinese roses

Technical Field

The invention belongs to the technical field of plant cultivation and breeding, and particularly relates to a method for improving the rooting capacity of Chinese rose cuttage through methyl jasmonate treatment.

Background

The Chinese rose belongs to the plants of Rosa of Rosaceae, and has rich flower color and unique flower type. The Chinese roses of most varieties can be opened in four seasons, and have the characteristic of long flowering period. In addition, the Chinese roses have various shapes, including micro Chinese roses, shrub Chinese roses, tree Chinese roses, vine Chinese roses and the like, can be used for landscaping, courtyard planting and indoor pot culture according to different requirements, and are excellent tree species for landscaping. Besides, China rose is one of four big cut flowers, and the economic value of China rose is self-evident.

At present, the traditional breeding method of Chinese rose is divided into sexual propagation method and asexual propagation method. In actual production, most of China roses are highly sterile, and even if a sexual propagation mode can be adopted for part of fertile China roses, the problems of long growth period, good character separation and the like exist. Therefore, in actual production, the propagation of the Chinese rose is usually carried out by asexual propagation. And cuttage is an important one of the asexual propagation modes of the Chinese rose. Cuttage is a vegetative propagation method for forming a complete plant body by using part of plant organs as propagules and inducing the propagules to generate adventitious roots or adventitious buds. The cuttage seedlings can quickly grow flowers while maintaining the excellent properties of the parents, and the disease resistance of the cuttage seedlings is strong, and the seedling stage is consistent.

Although the advantages of the cutting propagation are obvious, the cutting propagation is not suitable for all the Chinese rose varieties, and the problems of low rooting rate and low survival rate of the cutting propagation exist in part of the varieties. It has been shown that the survival rate of the vine rose without any hormone treatment is only 26.7% (han sheng, 2007). The low cutting survival rate severely limits the rapid propagation of the Chinese rose. Methods for promoting cuttage propagation of Chinese roses mostly focus on treatment with traditional auxin analogs, and other phytohormones are less researched.

Jasmonates are widely found in higher plants and are a cyclopentanone derivative with signal-mediated effect in plants. Jasmonic Acid (JA) participates in defense reaction of plants, can improve the stress resistance of the plants, and plays an important role in plant morphogenesis. Early studies concluded that methyl jasmonate had an inhibitory effect on root growth, and that Arabidopsis root growth was significantly inhibited on MS medium supplemented with methyl jasmonate (at a concentration of 5-50. mu. mol/L). Also, overexpression of the jasmonate methyltransferase gene (NTR1) in soybean resulted in a significant shortening of the primary root. Recent studies have shown low concentrations of methyl jasmonate (MeJA) (10)^-7mol/L) showed a promoting effect on the root and hypocotyl of peanuts (Huangshengqin, 2002). Overexpression of the NTR1 gene encoding jasmonate methyltransferase in soybean promotes the growth of lateral roots, but also inhibits primary root growth (XueR, 2007). Therefore, MeJA may also play an important role in the establishment of the root system of plants.

At present, no report that methyl jasmonate influences the cutting propagation of China rose exists. If the methyl jasmonate with proper concentration can promote the survival rate of cutting propagation, the cutting survival rate of the traditional Chinese rose can be greatly improved, and the method plays an important role in improving the propagation capacity of rare Chinese rose varieties.

Disclosure of Invention

In order to solve the problems of low survival rate and poor reproductive capacity of Chinese rose cutting rooting, the invention provides a method for improving Chinese rose cutting rooting, which is a method for promoting Chinese rose cutting rooting by a special plant growth regulator, namely methyl jasmonate; lays a foundation for the application of methyl jasmonate in the asexual propagation of China roses.

The technical scheme adopted by the invention for solving the technical problems is as follows:

a method for improving Chinese rose cutting rooting comprises the following steps:

(1) preparing a methyl jasmonate mother solution: firstly, preparing 1g/L methyl jasmonate aqueous solution from a methyl jasmonate emulsifier as mother solution;

(2) preparing cutting slips: selecting branches without plant diseases and insect pests and with good growth vigor as cutting mother branches and cutting the branches into cutting slips with proper size and character;

(3) and (3) cutting treatment: diluting methyl jasmonate mother liquor into working solution with proper concentration to soak the base part of the cutting shoot;

(4) preparing a seedbed: and preparing a seedling raising plate as a cutting seedbed. And adopting a matrix cultivation method to carry out seedling culture;

(5) tender branch cuttage: the treated cutting shoots are immediately cut in a seedling bed of a seedling raising plate;

(6) cuttage management: cutting seedling in artificial climate box, setting illumination period as long day and light intensity as weak light intensity, and covering to prevent rapid evaporation of water; and during the cutting seedling raising period, the substrate in the plug is kept moist but water cannot be accumulated.

Preferably, in the step (1), the preparation method of the methyl jasmonate mother liquor is as follows: dissolving a methyl jasmonate emulsifier in a methyl jasmonate absolute ethyl alcohol solution, diluting and dissolving the methyl jasmonate emulsifier by water to prepare a 1g/L aqueous jasmonate solution, wherein the volume ratio of the methyl jasmonate emulsifier to the methyl jasmonate absolute ethyl alcohol solution is 1: 9.

more preferably, in the step (1), the specific preparation method of the methyl jasmonate mother liquor is as follows: firstly, 100 mu L of methyl jasmonate emulsifier (with the purity of more than 95 percent and the density of 1g/mL) is dissolved in 900 mu L of prepared methyl jasmonate absolute ethyl alcohol solution (with the concentration of 0.1g/L), and then dissolved in 99mL of water to prepare 100mL of 1g/L methyl jasmonate aqueous solution. Preferably, in the step (2), the cutting preparation step is as follows: selecting a 'lunar powder' branch which is free from plant diseases and insect pests and has good growth vigor as a cutting mother branch, and cutting the branch into cutting slips of about 8-12 cm. 2-3 buds which are not germinated are reserved on each cutting shoot, the upper end of the first bud is cut into a flat opening at a position of about 1-1.5cm, 2-3 leaflets which are not germinated are reserved at the upper end of the first bud, the bottom end of the first bud is cut into a 45-degree inclined opening, and the cut openings are required to be smooth.

Preferably, in the step (3), the cutting processing step is as follows: preparing a methyl jasmonate mother solution prepared in the step (1) into a working solution, wherein the proper concentration is 0.10-1.00 mg/L; the concentration of the preferable working solution of methyl jasmonate is 0.25 mg/L; and (3) soaking the base part of the cutting shoot prepared in the step (2) with methyl jasmonate working solution, wherein the treatment time is 30 min.

Preferably, in the step (4), the bed preparation comprises the following specific steps: preparing a seedling raising tray as a cuttage seedling bed, weighing each component of the matrix according to a formula, uniformly mixing, then paving the seedling raising tray, and soaking the matrix by using water before cuttage; wherein, the matrix components and the corresponding volume ratio thereof are peat: coconut husk: perlite is 4:4: 2.

Preferably, in the step (4), the seedling raising tray is a 72-hole tray.

Preferably, in the step (5), the twig cutting method comprises the following steps: the cutting depth is about 2/3 of cutting slips, the first bud is 1-2cm above the substrate, and the 2 nd and 3 rd buds can be immersed in the substrate.

Preferably, in step (6), the cutting management method comprises: the cutting seedling is carried out in a climatic chamber, the illumination period is set to long day (16h illumination +8h darkness), the light intensity is set to weak light intensity (about 8000Lux), and the cover is covered to prevent the rapid evaporation of water. And during the cutting seedling raising period, the substrate in the plug is kept moist but water cannot be accumulated.

Under the 1g/L methyl jasmonate aqueous solution, the methyl jasmonate plays a positive role in the Chinese rose cuttage, the action mechanism of the methyl jasmonate possibly plays the same role in the mechanical damages such as girdling and the like, and the rooting capacity of the Chinese rose cuttage is improved by improving the activity of endogenous enzymes such as PPO and the like.

Advantageous effects

Compared with the prior art, the method for improving the cuttage rooting of the Chinese rose provided by the invention has the following beneficial effects:

(1) the invention aims to promote the cuttage rooting of Chinese roses by using a special plant growth regulator. According to the rooting characteristic of Chinese rose cuttage, the treatment concentrations promoted by methyl jasmonate with different concentrations are selected to treat the base parts of the cutting slips for 30min, the methyl jasmonate with different concentrations plays a role in promoting the Chinese rose cuttage, and the promotion effect of the methyl jasmonate with the concentration of 0.25mg/L on the cuttage rooting is the best.

(2) The method lays a foundation for applying methyl jasmonate to improving the cuttage capability of the Chinese rose; the method effectively promotes the generation and growth of the root system of the Chinese rose cutting, and greatly improves the reproductive capacity of the Chinese rose; can solve the dilemma of slow rooting rate and low survival rate in the current production of Chinese roses.

Drawings

FIG. 1 shows the effect of different concentrations of methyl jasmonate on the membership function of lunar powder;

FIG. 2 is a graph showing the effect of different example and comparative example treatment methods on the rooting of 'lunar powder' cutting.

Detailed Description

The present invention will be described in further detail with reference to examples. The reagents or instruments used are not indicated by manufacturers, and are regarded as conventional products which can be purchased in the market.

Example 1:

1. preparing a methyl jasmonate mother solution: firstly, preparing 1g/L methyl jasmonate aqueous solution from a methyl jasmonate emulsifier as mother solution. Firstly, dissolving 100 mu L of methyl jasmonate emulsifier (with the purity of more than 95 percent and the density of 1g/mL) in 900 mu L of prepared methyl jasmonate absolute ethanol solution (with the concentration of 0.1g/L), and then dissolving in 99mL of water to prepare 100mL of 1g/L methyl jasmonate aqueous solution which is used as mother solution for preparing subsequent working solution.

2. Preparing cutting slips: selecting a 'lunar powder' branch which is free from plant diseases and insect pests and has good growth as a cutting mother branch, cutting the cutting branch into about 8-12cm, reserving 2-3 buds which do not sprout on each cutting, cutting the upper end of the first bud by about 1-1.5cm into a flat opening, reserving 2-3 leaflets which do not sprout, cutting the bottom end into about 45-degree oblique openings, and cutting the openings smoothly to facilitate the formation of callus.

3. And (3) cutting treatment: preparing the mother liquor prepared in the step (1) into methyl jasmonate working solution with the concentration of 0.10mg/L, and soaking the base of the cutting for 30 min.

4. A72-hole tray was prepared as a cutting seedbed. And adopting a matrix cultivation method to carry out seedling culture. The formula of the substrate is peat: coconut husk: and (3) uniformly mixing perlite in a volume ratio of 4:4:2, and paving the mixture in a plug tray. And, the substrate should be soaked with water before cutting.

5. Tender branch cuttage: the treated cuttings are immediately inserted into a seedling bed with 72-hole trays. The cutting depth is about 2/3 of cutting shoot, ensuring that the first bud is 1-2cm above the substrate, and the 2 nd and 3 rd buds can be submerged into the substrate.

6. The cuttage management method comprises the following steps: the cutting seedling is carried out in a climatic chamber, the illumination period is set to long day (16h illumination +8h darkness), the light intensity is set to weak light intensity (about 8000Lux), and the cover is covered to prevent the rapid evaporation of water. And during the cutting seedling raising period, the substrate in the plug is kept moist but water cannot be accumulated.

Comparative example 1: the other steps are the same as the example 1, wherein in the step (3), the cutting is not treated by methyl jasmonate working solution, but the prepared cutting is soaked at the base of the cutting by clear water for 30 min.

Example 2: the other steps are the same as the example 1, wherein in the step (3), the mother liquor is prepared into methyl jasmonate working solution with the concentration of 0.25mg/L, and the base part of the cutting is soaked for 30 min.

Example 3: the other steps are the same as the example 1, wherein in the step (3), the mother liquor is prepared into methyl jasmonate working solution with the concentration of 0.50mg/L, and the base part of the cutting is soaked for 30 min.

Example 4: the other steps are the same as the example 1, wherein in the step (3), the mother liquor is prepared into methyl jasmonate working solution with the concentration of 1.00mg/L, and the base part of the cutting is soaked for 30 min.

The method used in examples 1 to 4 and comparative example 1 was different only in the concentration of the methyl jasmonate working solution. And (3) measuring related indexes of the root system 20 days after the cuttage is promoted, wherein the statistical data of each root system index is shown in table 1.

TABLE 1 influence of different concentrations of methyl jasmonate working solution on the correlation coefficient of the root system of lunar powder

The results show that examples 2 to 4 have significant promoting effects on the average root length, the average rooting rate, the average number of roots and the longest root length, compared with comparative example 1, except that the promoting effect of example 1 on the average root length is insignificant. In order to more intuitively see the promotion effect of different embodiments on the rooting capacity of the lunar meal, membership function value calculation is carried out on the root system indexes. The calculation results are shown in fig. 1. The results of the four root system indexes are integrated to show that the promotion effect of the embodiment 2 is optimal, so that the experiment result shows that the embodiment 2 can obviously promote the cuttage rooting capacity of the monthly dust, and promote the average root length, the root number and the longest root length while improving the average survival rate. The different examples and comparative examples promote the effect as shown in figure 2.

Example 5: the other steps are the same as example 1, wherein in the step (2) of preparing the cutting, the China rose variety 'lunar powder' is changed into 'Angira', and the other steps are the same.

Example 6: the other steps are the same as example 1, wherein in the step (2) of preparing cuttings, the number one of the other steps is changed from the number one of the Chinese rose variety 'lunar meal'.

The China rose varieties 'Angira' and 'Gedi I' in examples 5 and 6 are respectively full-fleshed China rose and Gedi China rose, and are different from the old China rose used in example 1. But the effects applied were similar and both significantly promoted the rooting effect of both varieties in the examples. Therefore, the present invention should be applicable to most of the rose varieties.

The protection of the present invention is not limited to the above embodiments. Variations and advantages that may occur to those skilled in the art may be incorporated into the invention without departing from the spirit and scope of the inventive concept and the scope of the appended claims is intended to be protected.

Claims

1. A method for improving cuttage rooting of Chinese roses is characterized by comprising the following steps:

(2) preparing cutting slips: selecting branches without plant diseases and insect pests and with good growth vigor as cutting mother branches to be cut into cutting slips;

(3) and (3) cutting treatment: diluting the methyl jasmonate mother liquor prepared in the step (1) into a working solution to soak the base of the cutting shoot;

(4) preparing a seedbed: preparing a seedling raising plate as a cutting seedbed, and raising seedlings by adopting a matrix cultivation method;

(5) tender branch cuttage: immediately cutting the cutting slips processed in the step (3) in a seedling bed of a seedling raising tray;

2. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (1), the preparation method of the methyl jasmonate mother liquor is as follows: dissolving a methyl jasmonate emulsifier in a methyl jasmonate absolute ethyl alcohol solution, diluting and dissolving the methyl jasmonate emulsifier by water, and preparing 1g/L methyl jasmonate aqueous solution; wherein the volume ratio of the methyl jasmonate emulsifier to the methyl jasmonate absolute ethyl alcohol solution is 1: 9.

3. the method for improving cuttage rooting of Chinese roses according to claim 2, wherein in the step (1), the preparation method of the methyl jasmonate mother liquor is as follows: firstly, 100 mu L of methyl jasmonate emulsifier with the purity of more than 95 percent and the density of 1g/mL is dissolved in 900 mu L of prepared methyl jasmonate absolute ethyl alcohol solution with the concentration of 0.1g/L, and then dissolved in 99mL of water to prepare 100mL of 1g/L methyl jasmonate aqueous solution.

4. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (2), the step of preparing the cutting slips is as follows: selecting branches without plant diseases and insect pests and with good growth vigor as cutting mother branches, and cutting the branches into cutting slips of about 8-12 cm; 2-3 buds which are not germinated are reserved on each cutting shoot, the upper end of the first bud is cut into a flat opening at a position of about 1-1.5cm, 2-3 leaflets which are not germinated are reserved at the upper end of the first bud, the bottom end of the first bud is cut into a 45-degree inclined opening, and the cut openings are required to be smooth.

5. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (3), the concentration of the working solution is 0.10-1.00 mg/L; the treatment time for the base of the cutting is 30 min.

6. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (4), the specific steps of seedbed preparation are as follows: preparing a seedling raising tray as a cuttage seedling bed, weighing each component of the matrix according to a formula, uniformly mixing, then paving the seedling raising tray, and soaking the matrix by using water before cuttage; wherein, the matrix components and the corresponding volume ratio thereof are peat: coconut husk: perlite =4:4: 2.

7. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (4), the seedling raising tray is a 72-hole plug tray.

8. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (5), the cutting depth is about 2/3 of the cutting slips, and the first bud is ensured to be 1-2cm above the substrate.

9. The method for improving cuttage rooting of Chinese roses according to claim 1, wherein in the step (6), the illumination period is set to 16h of illumination +8h of darkness, and the light intensity is set to 8000 Lux.