CN103053312A - Propagation method of Pterocarya stenoptera var. zhijiangensis - Google Patents
Propagation method of Pterocarya stenoptera var. zhijiangensis Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000305548 Pterocarya stenoptera Species 0.000 title abstract description 6
- 241000565359 Fraxinus chinensis Species 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
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- 235000015320 potassium carbonate Nutrition 0.000 claims description 3
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims description 3
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- YFNCATAIYKQPOO-UHFFFAOYSA-N thiophanate Chemical compound CCOC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OCC YFNCATAIYKQPOO-UHFFFAOYSA-N 0.000 description 2
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Abstract
A propagation method of Pterocarya stenoptera var. zhijiangensis includes artificial propagation steps of seed bed preparation, cutting collection, cuttage, post-cuttage management and the like. Vegetative seedling raising can be successfully performed to Pterocarya stenoptera var. zhijiangensis by the method, the rare species, Pterocarya stenoptera zhijiangensis, is continued, the survival rate of Pterocarya stenoptera zhijiangensis is more than 95%, and field seedlings grow neat with consistent properties.
Description
Technical field
The present invention relates to the mating system of a Plants, be specifically related to the mating system of a kind of Zhijiang Chinese ash.
Background technology
The Zhijiang Chinese ash is that the Juglandaceae Chinese ash belongs to deciduous tree, the Latin literary fame: Pterocarya stenoptera C.DC.Var. Zhijiangensis Z. E. Chao et C. J. Zheng.
Before more than 30 year, in the common willow stake of a century of Zhijiang, grow the seedling that one tree leaf, trunk and material all are different from common willow.Colleague's forestry expert introduction, this tree was found by former Zhijiang county Lin Kesuo chief Yan Xiaogui in 1979.On May 18th, 1979, technician of institute of county woods section compares with common willow at " strange tree " intercepting branch and leaf, finds that this tree exists many places different from general willow: the one, and general Poplar leaves is to life, this tree leaf is Dan Sheng; The 2nd, general Poplar Bark is chapped, and this tree bark smoother; The 3rd, general willow material is white in color, and this tree material is red; The 4th, the seed fruit wing of general willow upwarps, and the seed fruit wing of these seeds but is Eight characters parallel shape.Do not find corresponding species record and sample, this new discovery is reported step by step.
Nineteen eighty-two, Wuhan Institute of Zoology professor Zhao Zien leads expert group and rushes towards Zhou Hu village, Dong town, Zhijiang county, Hubei Province, takes away correlated samples, and is reported to Chinese forest-science academy together with survey report.Simultaneously, Chinese Academy of Sciences Wuhan plants is submitted to international plant association of the United Nations with new species name report.The discovery of variation willow causes the sensation of international plant educational circles immediately, and it is " Zhijiang Chinese ash " by definite designation soon." Zhijiang Chinese ash " belongs to the Juglandaceae deciduous tree, and it is new species,
At present the Zhijiang Chinese ash is rolled in kind of the mating system process of the test, at first adopted " seed is bred method ", but find that this species seed is asexual kind, and can't produce offspring; It two is to adopt " stone roller connects the method for breeding " test, also has no chance successfully because of natural windburn; It three is to adopt " method is bred in plant division " test, and this method can't divide because of the parent of these species again, cause breed unsuccessful; It four is to adopt " grafting and breeding method " test, because these species is strange, causes the affinity extreme difference and informs unsuccessfully.
Summary of the invention
For the deficiencies in the prior art, the purpose of this invention is to provide a kind of can successfully breeding by the Zhijiang Chinese ash, this is treasured species and is continued to make the Zhijiang Chinese ash, preserves the Zhijiang Chinese ash mating system in the characterizing gene storehouse of Zhijiang Chinese ash species research.
The object of the present invention is achieved like this: a kind of Zhijiang Chinese ash mating system may further comprise the steps:
(1) prepare in the front seedbed of cuttage
The front seedbed of cuttage should select that physical features is smooth, the soil is porous fertile and well-drained farmland, the seedbed is played before use sealing and is prevented from weed growth from spraying disinfection with liquor potassic permanganate, gets final product cuttage behind the covering with plastic film, and the pH value of soil remains on 7 ~ 8;
(2) plugged ear is prepared
Plugged ear lopping river Chinese ash branch, intercepted length are at 18 ~ 25cm, and stalwartness, the disease-free and lateral bud plumpness of assurance plugged ear, upper cut is from terminal bud 2cm when cutting fringe, and the upper end clip is level and smooth, does not stay burr, the lower end clip is cut into the shape of a hoof, the cuttage bar of intercepting should be In Shade, and the plugged ear of clip should be distinguished stem and top, divides just to empty and put well, and disinfection, for keeping its adequate water, also need put into aseptic running water and soak, add root-inducing powder in the aseptic running water and promote its Rapid Rooting;
(3) cuttage
Divide a cuttage with the plugged ear of soaking by cutting kind of a bar position in batches, keep ventilating, plugged ear is vertical with the seedbed during cuttage; Specific as follows:
With the matrix leveling, be chosen in cloudy day, morning or at dusk the plugged ear of handling well vertically inserted by seeding row spacing 15cm * 20cm before inserting, make its exposed parts be no more than 2/5ths of total length, stay a bud to reveal soil outside, water afterwards permeable and compacting; The complete rear sprinkling sterilization of cuttage; Adopt artificial vaporific water spray after 6 hours, guarantee soil moisture at 70%-75%, temperature is at 20-25 degree centigrade, will carry out bud picking to the sprouting that sprouts after the cuttage and prunes, and carry out foliage spray after two weeks of cuttage;
(4) transplant
15-20 days hardenings of cutting off the water before transplanting, to promote the coring healthy development and to improve the to external world adaptive capacity of high temperature and drought weather of nursery stock, to keep the complete of root system when transplanting lifting, with rising with planting with watering, keep nursery stock not dehydration in the transplanting process to divide, finish the Zhijiang Chinese ash and breed.
Root-inducing powder is selected IBA in the step (2), and the IBA hormone concentration of controlling in the aseptic running water is 100mg/L, soaks more than 6 hours.
Cutting medium on the seedbed selects the Changjiang river mud and cattle pen excrement humus soil 2.5:1 configuration to form.
Described seedbed base is not less than 45cm deeply.
Carrying out foliage spray after two weeks of cuttage is to enter the fast-growing after date Chinese ash to apply fertilizer 1 time about 15 days, and the used fertilizer that topdresses is for chasing after available nitrogen, potash fertilizer and di(2-ethylhexyl)phosphate zinc potassium.
Transplant and generally carry out in spring, should get with roots and soil seedling when transplant autumn.
Utilization the invention provides method the Zhijiang Chinese ash is bred, and Zhijiang Chinese ash survival rate reaches more than 95%, and field seedling gesture is neat, and character is consistent, show can successful bred by this method the Zhijiang Chinese ash.
The present invention is described in further detail below in conjunction with test example and embodiment.But this should be interpreted as that the scope of the above-mentioned theme of the present invention only limits to following embodiment, all technology that realizes based on content of the present invention all belong to scope of the present invention.
The Zhijiang Chinese ash mating system that the present invention enumerates may further comprise the steps:
(1) prepare in the seedbed
The front seedbed of cuttage should select that physical features is smooth, the soil is porous fertile and well-drained place.Cutting medium selects the Changjiang river mud and cattle pen excrement humus soil to form with weight ratio 2.5:1 configuration, and the Changjiang river mud is namely taken from the mud on the riverbed, the Changjiang river, and cattle pen excrement humus soil is the humus soil that has added cow dung, and the seedbed base is not less than 45cm deeply.The seedbed is played before use sealing and is prevented from weed growth from spraying disinfection with liquor potassic permanganate, and covering with plastic film got final product cuttage after 7 days, and the pH value of soil remains on 7 ~ 8.
(2) plugged ear is prepared
The Zhijiang Chinese ash branch at plugged ear intercepting 1 year age of growth, intercepted length are at 18 ~ 25cm, and the disease-free and lateral bud plumpness of robustness of assurance plugged ear, upper cut is from terminal bud 2cm when cutting fringe, and it is level and smooth that the upper end clip is wanted, and do not stay burr, the lower end clip is cut into the shape of a hoof, should note avoiding the otch fork to split.The cuttage bar of intercepting should be In Shade, and the plugged ear of clip should be distinguished the stem top, divides just to empty and put well, and with the thiophanate methyl sterilization, for keeping its adequate water, also need put into aseptic running water and soak more than 6 hours, and the adding root-inducing powder promotes its Rapid Rooting.Root-inducing powder is selected IBA, and the IBA hormone concentration of controlling in the aseptic running water is 100mg/L.
(3) cottage method
Divide a cuttage with the plugged ear of soaking by cutting kind of a bar position, physiological condition is more even in batches, and the new nursery stock of growth also can neat and consistent like this, and the distance of cuttage is advisable about with 15cm, keeps ventilating, and what pay special attention to is can not side slotting.Must make plugged ear vertical with the bed base during cuttage, evidence, slotting straight thousand, wound healing is good, and seedling growth is healthy and strong.
(4) management and cuttage
With the matrix leveling, be chosen in cloudy day, morning or at dusk the plugged ear of handling well vertically inserted by seeding row spacing 15cm * 20cm before inserting, make its exposed parts be no more than 2/5ths of total length, stay a bud to be exposed, water afterwards permeable and compacting.Complete rear the sprinkling with the import topsin of cuttage sterilized.Adopt artificial vaporific water spray after 6 hours, guarantee soil moisture at 70%-75%, temperature is at 20-25 degree centigrade.Watered once in general 7 days, reality is decided as the case may be.To carry out suitable bud picking to the sprouting that sprouts after the cuttage and prune, and after two weeks of cuttage, carry out foliage spray, and enter fast-growing after date fertilising in general about 15 days 1 time, mainly chase after available nitrogen, potash fertilizer and di(2-ethylhexyl)phosphate zinc potassium, fertilising is very favourable to cuttage rooting and growth, should carry out between the lights.
(5) transplant
Transplanting spring for well, autumn, transplanting should be got seedling with roots and soil, 15-20 days hardenings of must cutting off the water before transplanting, promote the coring healthy development and improve the to external world adaptive capacity of high temperature and drought weather of nursery stock, to keep the complete of root system when transplanting lifting, with rising with planting with watering, keep nursery stock not dehydration in the transplanting process to divide, transplanting should be between the lights or the cloudy day carry out.
Breed example 1: the applicant gathers the annual plugged ear of Zhijiang Chinese ash in March, 2008, and rugosity is at 1cm, and length is cut into 18 ~ 25cm, and upper cut is apart from bud 2cm; lower cut is cut into the shape of a hoof apart from bud 0.5cm, and otch is level and smooth; avoid splitting, it is not damaged to protect sprout, and processes with root-inducing powder.Begin cuttage in the seedbed of putting in order, plugged ear is all inserted in the soil, the upper end is equal with ground, treads on every side, and the rear plugged ear of watering 1 bud topmost bassets naturally, and seeding row spacing 15cm * 20cm guarantees ground moistening and sprays sterilization with topsin after the cuttage.Cuttage to the exhibition leaf, took root 30 days after, provide sufficient moisture, and timely chesson, for the growth of seedling provides better growing conditions, after two weeks of cuttage, carry out foliage spray, enter the fast-growing after date and apply fertilizer 1 time about 15 days.When seedling length to 20 ~ 30cm, keep 1 healthy and strong branch, remove other side shoots, sapling begins to enter the fast-growing phase after 60 days, loosens the soil, weeding.Stopped hardening in front 3 days in transplanting, keep coring complete when seedling is got in transplanting, water with getting at any time, keep nursery stock not dehydration in the transplanting process to divide, transplant and carry out at the cloudy day.Carry out field management by local planting habit, end in November, 2012, the Zhijiang Chinese ash diameter of a cross-section of a tree trunk 1.3 meters above the ground has reached 6cm, height 7m.Breed Zhijiang Chinese ash survival rate according to the inventive method and reach more than 95%, field seedling gesture is neat, and character is consistent, shows that the Zhijiang Chinese ash by the inventive method bred is successful.
The research of test example 1 Zhijiang Chinese ash plugged ear cut mode Rooting
1 materials and methods
1.1 test material
Test material is collected in 1 year newborn plugged ear of Zhou Hu village, Dong town, Zhijiang City Zhijiang Chinese ash, chooses stalwartness, not damaged and carries out the cuttage test without the plugged ear of damage by disease and insect.
1.2 test method
1.2.1 different incisions shape cuttage rooting relatively
With single face shape otch and two kinds of cut mode interceptings of horseshoe incision plugged ear, be divided into two groups and compare test respectively, other condition same treatment are done plugged ear to 1 year living high-quality seedling of Zhijiang Chinese ash and are carried out the cuttage root-taking situation relatively.
Results and analysis
2.1 different incisions shape cuttage rooting relatively
With different kerf plugged ears, the situation of taking root of plugged ear is as shown in table 1, and the result shows that the rooting rate of single face shape plugged ear is starkly lower than the rooting rate of shape of a hoof plugged ear, adopts horseshoe-shaped plugged ear coring many, and growth is fast, grow sturdy, otherwise then poorer.Beginning cuttage on March 12, the plugged ear of single face shape began to take root about April 29, and horseshoe-shaped plugged ear just began to take root about May 10.This is because horseshoe-shaped plugged ear can be accelerated Water Transportation, fresh-keeping and keep water balance and create conditions for plugged ear, the plugged ear wound area increases simultaneously, can make plugged ear obtain more nutriment, the wound inner thin-walled cell of transplanting a cutting obtains parallel fragmentation very soon, faster formation callus produces adventive root, and differentiation and development becomes a complete plant again.Different incisions plugged ear shape rooting rate situation sees Table one.
The research of test example 2 Zhijiang Chinese ash plugged ear HORMONE TREATMENT Rootings
1 materials and methods
1.1 test material
ABT root-inducing powder, 911 root elements and 3 kinds of materials of taking root of IBA
1.2 test method
1.2.1 hormon is processed the comparison of taking root
After Zhijiang Chinese ash plugged ear cut, with ABT root-inducing powder, 911 root elements and 3 kinds of materials of taking root of IBA, soaked 10 minutes respectively, 1 hour and 6 hours three kinds of modes are processed.Then adopt respectively three kinds of different concentration 100mg/L and 300mg/L, 600 mg/L carry out repeatedly horizontal repeated test.
Results and analysis
2.1 hormon is processed the comparison of taking root
As shown in table 2 with the different HORMONE TREATMENT situation of taking root, the result shows that it is the highest to soak 6 hours rooting rates, soak 10 minutes the poorest.This is because the moisture that the time of soaking plugged ear more of a specified duration absorbs is more abundant, has guaranteed to a certain extent the demand of plugged ear to moisture.Relatively ABT root-inducing powder, 911 root elements and 3 kinds of hormone impacts on taking root of IBA, HORMONE TREATMENT can improve the cuttage root-taking rate of Zhijiang Chinese ash effectively, and the rooting efficiency of IBA is best, and ABT root-inducing powder and 911 root element effects differ not obvious.Early take root about 6 days with the plugged ear that plugged ear average specific 911 root elements and the ABT root-inducing powder of IBA processing are processed.A common ground has been found in the processing of 3 kinds of hormone variable concentrations, and the effect that the 100mg/L low concentration is processed is more effective than 300mg/L and the processing of 800mg/L high concentration, illustrates that the Zhijiang Chinese ash is more suitable for processing in low concentration.From the situation of taking root of contrast, the Zhijiang Chinese ash belongs to the seeds that easily take root, and only needs to process a little just can significantly improve its rooting rate, and improvable thus is can suitably increase seeding row spacing when cuttage, in order to avoid affect its growth.Hormon is processed the rooting rate situation and is seen Table 2.
Test example 3 different plugged ears draw materials position and the seedling growth test research of different plugged ear age
1 materials and methods
1.1 test material
Each 50 of Zhijiang Chinese ash 1 year and perennial branch plugged ear and root plugged ears
1.2 test method
Position and the different plugged ear age comparison of growing seedlings 1.2.1 different plugged ears are drawn materials
Zhijiang Chinese ash 1 year and perennial branch plugged ear and each 50 of root plugged ears are divided into two groups, carry out the cuttage root-taking comparative trial behind the IBA immersion 6h with 100mg/L respectively, check different plugged ears draw materials position and the situation of growing seedlings at different plugged ear age.
Results and analysis
Position and the different plugged ear age comparison of growing seedlings 2.1 different plugged ears are drawn materials
As shown in table 3 with different plugged ears position and the different plugged ear age situation of growing seedlings of drawing materials, the result shows that the limb plugged ear of same age is higher than the root plugged ear, and 1 year living plugged ear of identical plugged ear is higher than perennial plugged ear rudiment power.The rudiment ability of taking root of 1 year living limb plugged ear of this explanation Zhijiang Chinese ash is the strongest.This is because the somatotropin content of limb plugged ear is high, and the rudiment of taking root is fast, and the somatotropin of root plugged ear is relatively low, and the plugged ear in 1 year advantage phase of being in growth is higher than the rudiment ability of taking root of perennial plugged ear simultaneously.The different plugged ears position of drawing materials sees Table 3 with the different plugged ear age situation of growing seedlings.
Claims (6)
1. Zhijiang Chinese ash mating system may further comprise the steps:
(1) prepare in the front seedbed of cuttage
The front seedbed of cuttage should select that physical features is smooth, the soil is porous fertile and well-drained farmland, the seedbed is played before use sealing and is prevented from weed growth from spraying disinfection with liquor potassic permanganate, gets final product cuttage behind the covering with plastic film, and the pH value of soil remains on 7 ~ 8;
(2) plugged ear is prepared
Plugged ear lopping river Chinese ash branch, intercepted length are at 18 ~ 25cm, and stalwartness, the disease-free and lateral bud plumpness of assurance plugged ear, upper cut is from terminal bud 2cm when cutting fringe, and the upper end clip is level and smooth, does not stay burr, the lower end clip is cut into the shape of a hoof, the cuttage bar of intercepting should be In Shade, and the plugged ear of clip should be distinguished stem and top, divides just to empty and put well, and disinfection, for keeping its adequate water, also need put into aseptic running water and soak, add root-inducing powder in the aseptic running water and promote its Rapid Rooting;
(3) cuttage
Divide a cuttage with the plugged ear of soaking by cutting kind of a bar position in batches, keep ventilating, plugged ear is vertical with the seedbed during cuttage; Specific as follows:
With the matrix leveling, be chosen in cloudy day, morning or at dusk the plugged ear of handling well vertically inserted by seeding row spacing 15cm * 20cm before inserting, make its exposed parts be no more than 2/5ths of total length, stay a bud to reveal soil outside, water afterwards permeable and compacting; The complete rear sprinkling sterilization of cuttage; Adopt artificial vaporific water spray after 6 hours, guarantee soil moisture at 70%-75%, temperature is at 20-25 degree centigrade, will carry out bud picking to the sprouting that sprouts after the cuttage and prunes, and carry out foliage spray after two weeks of cuttage;
(4) transplant
15-20 days hardenings of cutting off the water before transplanting, to promote the coring healthy development and to improve the to external world adaptive capacity of high temperature and drought weather of nursery stock, to keep the complete of root system when transplanting lifting, with rising with planting with watering, keep nursery stock not dehydration in the transplanting process to divide, finish the Zhijiang Chinese ash and breed.
2. Zhijiang Chinese ash mating system according to claim 1 is characterized in that: root-inducing powder is selected IBA in the step (2), and the IBA hormone concentration of controlling in the aseptic running water is 100mg/L, soaks more than 6 hours.
3. Zhijiang Chinese ash mating system according to claim 1 is characterized in that: the cutting medium on the seedbed selects the Changjiang river mud and cattle pen excrement humus soil 2.5:1 configuration to form.
4. Zhijiang Chinese ash mating system according to claim 1, it is characterized in that: described seedbed base is not less than 45cm deeply.
5. Zhijiang Chinese ash mating system according to claim 1, it is characterized in that: carrying out foliage spray after two weeks of cuttage is to enter the fast-growing after date Chinese ash to apply fertilizer 1 time about 15 days, and the used fertilizer that topdresses is for chasing after available nitrogen, potash fertilizer and di(2-ethylhexyl)phosphate zinc potassium.
6. Zhijiang Chinese ash mating system according to claim 1 is characterized in that: transplant and generally carry out in spring, should get with roots and soil seedling when transplant autumn.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103477827A (en) * | 2013-09-06 | 2014-01-01 | 巴中市光雾山植物研究所 | Red maple cuttage propagation method |
| CN107094566A (en) * | 2017-04-05 | 2017-08-29 | 周兴虎 | A kind of willow cuttage seedling raising method |
| CN107736178A (en) * | 2017-11-14 | 2018-02-27 | 佴辉 | A kind of Chinese ash full sunshine and spray cuttage rapid propagating method |
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| CN1418534A (en) * | 2002-12-31 | 2003-05-21 | 黄立海 | Method for cultivating wingnut trees |
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| 许一心等: "枫杨嫩枝扦插繁殖试验", 《江苏林业科技》, no. 02, 30 April 2010 (2010-04-30) * |
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| CN103477827A (en) * | 2013-09-06 | 2014-01-01 | 巴中市光雾山植物研究所 | Red maple cuttage propagation method |
| CN103477827B (en) * | 2013-09-06 | 2015-03-25 | 巴中七彩林业科技有限公司 | Red maple cuttage propagation method |
| CN107094566A (en) * | 2017-04-05 | 2017-08-29 | 周兴虎 | A kind of willow cuttage seedling raising method |
| CN107736178A (en) * | 2017-11-14 | 2018-02-27 | 佴辉 | A kind of Chinese ash full sunshine and spray cuttage rapid propagating method |
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| CN103053312B (en) | 2014-08-20 |
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