CN107810857A - A kind of detoxicated ginger method for tissue culture - Google Patents
A kind of detoxicated ginger method for tissue culture Download PDFInfo
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- CN107810857A CN107810857A CN201711408292.2A CN201711408292A CN107810857A CN 107810857 A CN107810857 A CN 107810857A CN 201711408292 A CN201711408292 A CN 201711408292A CN 107810857 A CN107810857 A CN 107810857A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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Abstract
The invention discloses a kind of detoxicated ginger method for tissue culture, comprise the following steps：（1）Selection：Choose without rotten disease-free, healthy and strong ginger stem tuber；（2）Vernalization：The ginger stem tuber chosen is subjected to vernalization；（3）Stem apex detoxification：Ginger bud explant is cleaned, sterilized, peels off to be seeded on culture medium I and is cultivated；（4）Proliferation and subculture：Aseptic seedling is inoculated into progress proliferation and subculture culture on corresponding culture medium；（5）Culture of rootage；（6）Tame hardening.The beneficial effects of the invention are as follows：Ginger can play a part of detoxification, fast numerous, Germ-plasma resources protection, quality-improving, ginger quality and quality is greatly improved by tissue cultures；The medium component that control differentiation, subculture grow, taken root etc. is determined in tissue cultures, the optimum optimization selection of the species of growth regulatory substance, concentration and condition of culture is determined, the technology barriers captured in Ginger Tissue incubation, reach good culture effect.
Description
Technical field
The invention belongs to field of plant tissue culture technique, more particularly to a kind of detoxicated ginger method for tissue culture.
Background technology
Ginger is also known as ginger, and its edible part is the root-like stock of ginger, in addition to containing zingerone, zingerol and ginger oil alcohol, is also contained
Protein, sugar, crude fat, vitamin and abundant molysite.Ginger due to its is nutritious, medicinal effects are good, economic value is high,
Resistance to accumulating, is increasingly taken seriously in recent years, and cultivated area and demand constantly expand, and export volume is also in increase trend year by year.
Do not bloomed due to ginger or seldom bloomed, it is impossible to carried out breeding using generative propagation, a small number of kinds can only be leaned on long-term
Vegetative propagation, cause a variety of viruses of ginger plant cylinder accumulation, yield declines, and quality deterioration, resistance declines.Ginger Germplasm
Breeding and preserving also rely primarily on and make propagating materials with kind of ginger and carry out field planting every year, breeding coefficient is low, and expends greatly
Measure human and material resources and financial resources.
With the development of biotechnology, plant tissue culture technique has a great development in recent years, large quantities of plants
Tissue cultures obtain success, and achieve significant economic benefit and social benefit, and tissue culture technique has been also applied to life
In the production of ginger, good economic benefit is achieved, increasingly shows powerful application value in ginger production.Using tissue
Culture technique can carry out quick breeding to meet to produce needs to excellent ginger kind, and tissue is carried out with the different explants of ginger
Culture studies also have some reports, but production cost height be present, and primary inoculation pollution rate is high, Vitrification, differentiation potency
Power is from declining the problem such as low with survival rate.
The content of the invention
The defects of purpose of the present invention is to overcome above-mentioned Ginger Tissue culture, the detoxification method for tissue culture to ginger enter
Row systematic research, by many experiments, obtain a kind of easy to operate, raising Ginger Yield and the cultural method of quality.
To achieve the above object, the present invention uses following technical proposals：
A kind of detoxicated ginger method for tissue culture, including：
（1）Selection：Choose without rotten disease-free, healthy and strong ginger stem tuber；
（2）Vernalization：The ginger stem tuber chosen is placed in no light room, 25 ~ 30 DEG C of temperature is maintained, humidity 40 ~ 50%, places 7
~ 10d, now 1 ~ 2cm of ginger bud explant；
（3）Stem apex detoxification：By step（2）In obtained ginger bud explant rinsed well using flowing water, be dipped in Tween-80
10 ~ 20min in the aqueous solution, then Tween-80 is rinsed well with circulating water；The bud explant handled well is used into 75% alcohol
Sterilize 1 ~ 5min, 10 ~ 20min of 1%HgCl solution disinfections；Peel off stem apex；The stem apex peeled off is seeded on culture medium I, placed
It is 2500 ~ 3500Lux, cultivates 40 ~ 60d in 25 ~ 28 DEG C of temperature, daily 7 ~ 12h of illumination, intensity of illumination；
（4）Proliferation and subculture：Selecting step（3）In obtained aseptic seedling be inoculated on culture medium II and carry out Multiplying culture, train
Foster condition is：25 ~ 26 DEG C of temperature, daily 10 ~ 12h of illumination, intensity of illumination are 3000 ~ 5000Lux, 20 ~ 30d of culture；Cultivating
Above-mentioned breeding is repeated on base III；
（5）Take root：Healthy and strong 2 ~ the 3cm of plant height of growing way ginger seedling is chosen, is seeded in culture medium IV, is positioned over temperature 25
~ 26 DEG C, daily 10 ~ 12h of illumination, intensity of illumination be 5000 ~ 7000Lux environment in, cultivate 20d；
（6）Tame hardening：By step（5）Obtained well developed root system, 8 ~ 10cm of plant height ginger seedling, which is transferred in small temperature canopy, to be carried out
Domestication, shade, keep ginger seedling after temperature is gradually decremented to 18 ~ 20 DEG C, 15 days after being 25 ~ 26 DEG C, 7 days in small temperature canopy from group
Knit in culture medium and remove, the culture medium of root is cleaned up, planted in substrate soil；
Wherein：Culture medium I be added in B5 medium 0.5mg/LIBA, 1.0mg/LKT, 0.5mg/L activated carbon, 30% sucrose and
4.5g/L agar；
Culture medium II is that 2.0mg/LKT, 0.5mg/LNAA, 30% sucrose and 4.5g/L agar are added in B5 medium；
Culture medium III is that 0.1mg/LNAA, 0.5mg/LKT, 30% sucrose and 4.5g/L agar are added in B5 medium；
Culture medium IV is that 0.5mg/LIBA, 20% sucrose and 4.5g/L agar are added in B5 medium；
As optimization, a kind of detoxicated ginger method for tissue culture also includes antibiotic treatment, in step（3）Stem apex detoxification
The one or more added in the culture medium I used in antibiotics penicillin, kanamycins, streptomycin sulphate, rifampin.
As optimization, the addition of the antibiotic is 10mg/L ~ 1g/L.
As preferable, the step（3）Middle Tween-80 concentration of aqueous solution is 2 ~ 4%.
As optimization, the step（6）In, after tissue culturing seedling takes out from blake bottle, early stage is maintained in small temperature canopy
Humidity is more than 90%, is gradually successively decreased, until film is taken off on small temperature canopy.
B5 medium is to be designed by Gamborg et al. nineteen sixty-eight, by inorganic nutrients thing, carbon source, vitamin, growth regulating
The class material composition of material and Organic additives etc. five.
Activated carbon：The detrimental metabolic concentration of tissue culture can be reduced, cell growth is favourable.Activated carbon is charcoal powder
The mealy structure of broken processed formation, its short texture, hole is big, and water absorbing capacity is strong, there is very strong suction-operated, its particle
Size decides adsorption capacity, and its smaller adsorption capacity of granularity is bigger.It can adsorb the macromoleculars such as apolar substance and pigment
Contained impurity in material, including agar, phenol, quinones substance and the sucrose of culture secretion are in 5- caused by autoclave sterilization
Hydroxymethylfurfural and hormone etc..
Heteroauxin (IBA)：The Endogenous auxin of generally existing in a kind of plant, belong to Benzazole compounds, You Mingzhuo
Long element, auxin, different growth hormone, are a kind of organic matters, stable to visible ray, have duality, plant difference to plant growth
Position is different to its susceptibility, and general root is more than stem, and different plants are also different to its sensitivity.
6-Furfurylaminopurine (KT)：It is a kind of non-natural basic element of cell division, promotes cell differentiation, division, growth；Lure
Callus is led to sprout；Release apical dominance；Promote germination, break the dormancy of lateral bud；Delaying Leaf-Senescence and plant
Early ageing；Adjust the transport of nutriment；Promote solid；Floral bud induction breaks up；Adjust Stoma of Leaves opening etc..
Methyl α-naphthyl acetate (NAA)：It is the plant growth regulator for promoting plant root growth, and the intermediate of NAD.
Methyl α-naphthyl acetate is used as plant growth regulator, has and promotes cell division and expansion, and induced synthesis adventitious root, increase is beared fruit, prevented
Shedding etc., can be with nutrition stream transporting to site of action.
Agar：The most useful characteristic of agar is that the temperature difference between its condensation point and fusing point is very big.It needs to add in water
Just start to melt when heat is to 95 DEG C, just start to solidify when the solution temperature after fusing need to drop to 40 DEG C, so it is to prepare solid
The best coagulator of culture medium.The solid medium prepared with agar, is used for high-temperature cultivation and non-fusible, in solidification
During preceding inoculation, culture will not also be scalded dead.Therefore, agar is to prepare most widely used one kind in various biological mediums to coagulate
Gu agent.
Sucrose：Sucrose is in addition to energy supply, moreover it is possible to which evoked callus tissue breaks up again；During culture medium high-temperature sterilization, sugarcane
Sugar is more stable and is not easy to be carbonized；Contain invertase in plant cell, sucrose can preferably remain hypotonic in culture medium
Environment, the osmotic pressure that sucrose is formed can keep relative stability for a long time, can reduce the pollution of microorganism to a certain extent.
The beneficial effects of the invention are as follows：
1. ginger can be played a part of detoxification, fast numerous, Germ-plasma resources protection, quality-improving, significantly be carried by tissue cultures
High ginger quality and quality, expand the planting scale of ginger.
2. the medium component that control differentiation, subculture grow, taken root etc. is determined in tissue cultures, growth has been determined
The optimum optimization selection of the species of Auto-regulator, concentration and condition of culture, the technology captured in Ginger Tissue incubation
Barrier, reach good culture effect.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained under the premise of creative work is not made, belong to the model that the present invention protects
Enclose.The experimental method of unreceipted actual conditions in the following example, generally according to normal condition, or according to proposed by manufacturer
Condition, unless stated otherwise, ratio and percentage are based on weight.
The raw materials used embodiment of the present invention is commercially available.
Embodiment 1, a kind of detoxicated ginger method for tissue culture, a kind of detoxicated ginger method for tissue culture, including：
（1）Selection：Choose without rotten disease-free, healthy and strong ginger stem tuber；
（2）Vernalization：The ginger stem tuber chosen is placed in no light room, 28 DEG C of temperature is maintained, humidity 50%, places 7d, now
1 ~ 2cm of ginger bud explant；
（3）Stem apex detoxification：By step（2）In obtained ginger bud explant rinsed well using flowing water, be dipped in Tween-80
15min in the aqueous solution, the Tween-80 concentration of aqueous solution are 2%, i.e., 1g Tween-80s are added in 500mL water；Then use circulating water
Tween-80 is rinsed well；The bud explant handled well is used into 75% alcohol disinfecting 5min, 1%HgCl solution disinfection 10min；
Peel off stem apex；The stem apex peeled off is seeded on culture medium I, culture medium I be B5 medium in add 0.5mg/LIBA,
1.0mg/LKT, 0.5mg/ activated carbon, 30% sucrose and 4.5g/L agar, and 10mg/L penicillin is additionally added, it is positioned over temperature
26 DEG C, daily illumination 12h, intensity of illumination 2500Lux, culture 50d；
（4）Proliferation and subculture：Selecting step（3）In obtained aseptic seedling be inoculated on culture medium II and carry out Multiplying culture, train
It is to add 2.0mg/LKT, 0.5mg/LNAA, 30% sucrose and 4.5g/L agar, condition of culture in B5 medium to be to support base II：Temperature
26 DEG C, daily illumination 10h, intensity of illumination 4000Lux, culture 30d；Above-mentioned breeding, culture are repeated on culture medium III
Base III is that 0.1mg/LNAA, 0.5mg/LKT, 30% sucrose and 4.5g/L agar are added in B5 medium；
（5）Take root：Healthy and strong 2 ~ the 3cm of plant height of growing way ginger seedling is chosen, is seeded in B5 medium and adds 0.5mg/
In LIBA, 20% sucrose and 4.5g/L agar culture medium IV, being positioned over 25 DEG C of temperature, daily illumination 11h, intensity of illumination is
In 7000Lux environment, 20d is cultivated；
（6）Tame hardening：By step（5）Obtained well developed root system, 8 ~ 10cm of plant height ginger seedling, which is transferred in small temperature canopy, to be carried out
Domestication, shade, keep ginger seedling after temperature is gradually decremented to 20 DEG C, 15 days after being 26 DEG C, 7 days in small temperature canopy from tissue cultures
Removed in base, the culture medium of root is cleaned up, planted in substrate soil；It is preceding after tissue culturing seedling takes out from blake bottle
It is more than 90% that phase, which maintains humidity in small temperature canopy, is gradually successively decreased, until film is taken off completely on small temperature canopy.By this detoxicated ginger
More than 99%, Ginger Yield and quality are all greatly improved the ginger shoot survival percent that method for tissue culture obtains.
Culture medium used in embodiment 2 and embodiment 3 is same as Example 1, repeats no more, difference is：
Embodiment 2
A kind of detoxicated ginger method for tissue culture, including：
（1）Selection：Choose without rotten disease-free, healthy and strong ginger stem tuber；
（2）Vernalization：The ginger stem tuber chosen is placed in no light room, 25 DEG C of temperature is maintained, humidity 45%, places 10d, this
1 ~ 2cm of Shi Shengjiang bud explants；
（3）Stem apex detoxification：By step（2）In obtained ginger bud explant rinsed well using flowing water, being dipped in concentration is
10min in the 3% Tween-80 aqueous solution, is then rinsed well Tween-80 with circulating water；The bud explant handled well is used
75% alcohol disinfecting 3min, 1%HgCl solution disinfection 20min；Peel off stem apex；The stem apex peeled off is seeded on culture medium I,
5mg/L kanamycins, 5mg/L streptomycin sulphates are additionally added on culture medium I, is positioned over 25 DEG C of temperature, daily illumination 10h, light
It is 3500Lux according to intensity, culture 40d；
（4）Proliferation and subculture：Selecting step（3）In obtained aseptic seedling be inoculated on culture medium II and carry out Multiplying culture, train
Foster condition is：26 DEG C of temperature, daily illumination 12h, intensity of illumination 3000Lux, culture 25d；It is repeatedly above-mentioned on culture medium III
Breeding；
（5）Take root：Healthy and strong 2 ~ the 3cm of plant height of growing way ginger seedling is chosen, is seeded in culture medium IV, is positioned over temperature 26
DEG C, daily illumination 10h, intensity of illumination be 6000Lux environment in, cultivate 20d；
（6）Tame hardening：By step（5）Obtained well developed root system, 8 ~ 10cm of plant height ginger seedling, which is transferred in small temperature canopy, to be carried out
Domestication, shade, keep ginger seedling after temperature is gradually decremented to 19 DEG C, 15 days after being 25 DEG C, 7 days in small temperature canopy from tissue cultures
Removed in base, the culture medium of root is cleaned up, planted in substrate soil；It is preceding after tissue culturing seedling takes out from blake bottle
It is more than 90% that phase, which maintains humidity in small temperature canopy, is gradually successively decreased, until film is taken off completely on small temperature canopy.By this detoxicated ginger
More than 99%, Ginger Yield and quality are all greatly improved the ginger shoot survival percent that method for tissue culture obtains.
Embodiment 3
A kind of detoxicated ginger method for tissue culture, including：
（1）Selection：Choose without rotten disease-free, healthy and strong ginger stem tuber；
（2）Vernalization：The ginger stem tuber chosen is placed in no light room, 30 DEG C of temperature is maintained, humidity 40%, places 8d, now
1 ~ 2cm of ginger bud explant；
（3）Stem apex detoxification：By step（2）In obtained ginger bud explant rinsed well using flowing water, being dipped in solubility is
20min in the 4% Tween-80 aqueous solution, is then rinsed well Tween-80 with circulating water；The bud explant handled well is used
75% alcohol disinfecting 1min, 1%HgCl solution disinfection 15min；Peel off stem apex；The stem apex peeled off is seeded on culture medium I, volume
Outer addition 20mg/L rifampins, it is positioned over 28 DEG C of temperature, daily illumination 7h, intensity of illumination 3000Lux, culture 60d；
（4）Proliferation and subculture：Selecting step（3）In obtained aseptic seedling be inoculated on culture medium II and carry out Multiplying culture, train
Foster condition is：25 DEG C of temperature, daily illumination 11h, intensity of illumination 5000Lux, culture 20d；It is repeatedly above-mentioned on culture medium III
Breeding；
（5）Take root：Healthy and strong 2 ~ the 3cm of plant height of growing way ginger seedling is chosen, is seeded in culture medium IV, is positioned over temperature 25
DEG C, daily illumination 12h, intensity of illumination be 5000Lux environment in, cultivate 20d；
（6）Tame hardening：By step（5）Obtained well developed root system, 8 ~ 10cm of plant height ginger seedling, which is transferred in small temperature canopy, to be carried out
Domestication, shade, keep ginger seedling after temperature is gradually decremented to 18 DEG C, 15 days after being 26 DEG C, 7 days in small temperature canopy from tissue cultures
Removed in base, the culture medium of root is cleaned up, planted in substrate soil；It is preceding after tissue culturing seedling takes out from blake bottle
It is more than 90% that phase, which maintains humidity in small temperature canopy, is gradually successively decreased, until film is taken off completely on small temperature canopy.By this detoxicated ginger
More than 99%, Ginger Yield and quality are all greatly improved the ginger shoot survival percent that method for tissue culture obtains.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
God any modification, equivalent substitution and improvements made etc., should be included in the scope of the protection with principle.
Claims (5)
- A kind of 1. detoxicated ginger method for tissue culture, it is characterised in that：Including：（1）Selection：Choose without rotten disease-free, healthy and strong ginger stem tuber；（2）Vernalization：The ginger stem tuber chosen is placed in no light room, 25 ~ 30 DEG C of temperature is maintained, humidity 40 ~ 50%, places 7 ~ 10d, now 1 ~ 2cm of ginger bud explant；（3）Stem apex detoxification：By step（2）In obtained ginger bud explant rinsed well using flowing water, be dipped in Tween-80 10 ~ 20min in the aqueous solution, then Tween-80 is rinsed well with circulating water；The bud explant handled well is used into 75% alcohol Sterilize 1 ~ 5min, 10 ~ 20min of 1%HgCl solution disinfections；Peel off stem apex；The stem apex peeled off is seeded on culture medium I, placed In 25 ~ 28 DEG C of temperature, daily 7 ~ 12h of illumination, the culture environment that intensity of illumination is 2500 ~ 3500Lux, 40 ~ 60d is cultivated；（4）Propagation, subculture：Selecting step（3）In obtained aseptic seedling be inoculated on culture medium II and carry out Multiplying culture, Condition of culture is：25 ~ 26 DEG C of temperature, daily 10 ~ 12h of illumination, intensity of illumination are 3000 ~ 5000Lux, 20 ~ 30d of culture；Training Support and above-mentioned breeding is repeated on base III；（5）Take root：Healthy and strong 2 ~ the 3cm of plant height of growing way ginger seedling is chosen, is seeded in culture medium IV, is positioned over temperature 25 ~ 26 DEG C, daily 10 ~ 12h of illumination, intensity of illumination be 5000 ~ 7000Lux environment in, cultivate 20d；（6）Tame hardening：By step（5）Obtained well developed root system, 8 ~ 10cm of plant height ginger seedling, which is transferred in small temperature canopy, to be carried out Domestication, shade, keep ginger seedling after temperature is gradually decremented to 18 ~ 20 DEG C, 15 days after being 25 ~ 26 DEG C, 7 days in small temperature canopy from group Knit in culture medium and remove, the culture medium of root is cleaned up, planted in substrate soil；Wherein：Culture medium I is that 0.5mg/LIBA, 1.0mg/LKT, 0.5mg/L activated carbon, 30% sucrose and 4.5g/L are added in B5 medium Agar；Culture medium II is that 2.0mg/LKT, 0.5mg/LNAA, 30% sucrose and 4.5g/L agar are added in B5 medium；Culture medium III is that 0.1mg/LNAA, 0.5mg/LKT, 30% sucrose and 4.5g/L agar are added in B5 medium；Culture medium IV is that 0.5mg/LIBA, 20% sucrose and 4.5g/L agar are added in B5 medium.
- A kind of 2. detoxicated ginger method for tissue culture according to claim 1, it is characterised in that：A kind of detoxicated ginger Method for tissue culture also includes antibiotic treatment, in step（3）Antibiotic mould is added in the culture medium I that stem apex detoxification is used One or more in element, kanamycins, streptomycin sulphate, rifampin.
- A kind of 3. detoxicated ginger method for tissue culture according to claim 2, it is characterised in that：The addition of the antibiotic Measure as 10mg/L ~ 1g/L.
- A kind of 4. detoxicated ginger method for tissue culture according to claim 1, it is characterised in that：The step（3）In tell Warm -80 concentration of aqueous solution are 2 ~ 4%.
- A kind of 5. detoxicated ginger method for tissue culture according to claim 1, it is characterised in that：The step（6）In, group Knit after cultivating seedling takes out from blake bottle, it is more than 90% to maintain humidity in small temperature canopy early stage, is gradually successively decreased, until on small temperature canopy Film is taken off completely.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109042317A (en) * | 2018-06-11 | 2018-12-21 | 安徽省兰香缘农业生态发展有限责任公司 | A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred |
| CN111771725A (en) * | 2020-07-31 | 2020-10-16 | 潍坊兴旺生物种业有限公司 | Optimized ginger virus-free seedling regeneration propagation method and development of industrialization process thereof |
| TWI712361B (en) * | 2018-06-01 | 2020-12-11 | 謝瑞裕 | Method of ginger plant cultivation |
| CN115735767A (en) * | 2022-11-10 | 2023-03-07 | 潍坊峡兴农业科技有限公司 | Rapid propagation method of sugar-free virus-free ginger seedlings |
| CN115777531A (en) * | 2022-09-19 | 2023-03-14 | 一禾亿株生物种业(深圳)有限公司 | Ginger tissue culture seedling method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930951A (en) * | 2006-09-29 | 2007-03-21 | 徐坤 | Method of cultivating detoxicated ginger seedling |
| CN104585027A (en) * | 2014-12-24 | 2015-05-06 | 徐国华 | Seedling hardening method for ginger tissue culture seedlings |
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2017
- 2017-12-22 CN CN201711408292.2A patent/CN107810857A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1930951A (en) * | 2006-09-29 | 2007-03-21 | 徐坤 | Method of cultivating detoxicated ginger seedling |
| CN104585027A (en) * | 2014-12-24 | 2015-05-06 | 徐国华 | Seedling hardening method for ginger tissue culture seedlings |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI712361B (en) * | 2018-06-01 | 2020-12-11 | 謝瑞裕 | Method of ginger plant cultivation |
| CN109042317A (en) * | 2018-06-11 | 2018-12-21 | 安徽省兰香缘农业生态发展有限责任公司 | A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred |
| CN111771725A (en) * | 2020-07-31 | 2020-10-16 | 潍坊兴旺生物种业有限公司 | Optimized ginger virus-free seedling regeneration propagation method and development of industrialization process thereof |
| CN115777531A (en) * | 2022-09-19 | 2023-03-14 | 一禾亿株生物种业(深圳)有限公司 | Ginger tissue culture seedling method |
| CN115735767A (en) * | 2022-11-10 | 2023-03-07 | 潍坊峡兴农业科技有限公司 | Rapid propagation method of sugar-free virus-free ginger seedlings |
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