CN109042317A - A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred - Google Patents
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred Download PDFInfo
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- CN109042317A CN109042317A CN201810592556.2A CN201810592556A CN109042317A CN 109042317 A CN109042317 A CN 109042317A CN 201810592556 A CN201810592556 A CN 201810592556A CN 109042317 A CN109042317 A CN 109042317A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0256—Ground coverings
- A01G13/0281—Protective ground coverings for individual plants, e.g. for plants in pots
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
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- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides a kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, detoxicated ginger is carried out first with the method that heat treatment, Shoot Tip Culture and chemical agent combine, then culture medium culture is carried out using the ginger bud after detoxification, it can be achieved that the proliferation of ginger test tube seedling, taking root and a step culture of strong sprout；Ginger seedling is subjected to cutting propagation using straight cutting method with plastic film after cultivation, by rationally controlling environment conditioning after cuttage pre-treatment, cuttage, water and fertilizer management etc., compared with normal cutting propagation, straight cutting overlay film is able to maintain soil moisture and air humidity, inhibit weeds, seedling root system is promoted to develop in advance, rooting rate significantly improves, and number of days of taking root shortens 3-5 days, and seedling growing way and quality are also improved, it eliminates simultaneously and builds Small plastic shed, carries out repeatedly the management works such as spraying daily, it improves labor efficiency, relative reduction seedling cost is increased economic efficiency.
Description
Technical field
The present invention relates to the sides that field of planting more particularly to a kind of self tissue culture bud straight cutting overlay film of detoxification ginger are bred
Method.
Background technique
Ginger is the herbaceos perennial of Zingiber zingiber, annual vegetable, while having edible and medical value, therefore
There is very big demand in daily bread and medicine field.As one of spice class flavouring, the peculiar flavour of ginger is undesirable
It in generation, is all widely used in many food processing process, there is very excellent market development prospect for many years.Except this it
Outside, ginger also has higher easy to raise, yield, storage tolerance and good economic performance, so correlation of the people to ginger
Research is also abnormal extensive.As the export market development of ginger in recent years is become better and better, cultivated area also increasingly increases, tradition
Ginger type of rearing be underground tubers vegetative propagation, the shortcomings that this method is that breeding potential is not high, and long with soil
Phase contact is easy to that all kinds of lesions occur, and is difficult to reduce such lesion by drug.In order to further increase its yield and improve
Ginger growth quality, many people take up to develop more training methods, wherein using effect is best and overall cost most
Low training method is tissue culture method.
The ginger seedling obtained using tissue culture method is subjected to cuttage, most biggish seedling can be bred as in a short time, and
It is able to maintain original breediness, the plant of culture is faster than tree seedling growth, but cuttage seeding, without main root, root system is often weak compared with tree seedling,
It is often shallow root.Under normal circumstances, after cuttage and seedling culture is taken root, then it is colonized the seedling-slowing stage for also needing 3~5d, and straight cutting field planting can save shifting
Cultivation and slow seedling process shorten from cuttage to the time being out of the garden.
Summary of the invention
The purpose of the present invention is to provide the methods that a kind of self tissue culture bud straight cutting overlay film of detoxification ginger is bred, with solution
Certainly above-mentioned technical problem.
The present invention using following technical scheme in order to solve the above technical problems, realized:
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, comprising the following specific steps
(1) yellow ginger is placed in clean sand table, covers the fine sand of about 3 ㎝ thickness above, water spray keeps river sand wet, with sand table
Inside ponding is not advisable, and is covered on river sand with wet towel, is placed in culture in artificial lighting incubator, long to 1-2 ㎝ to bud after 4-6 weeks
When take out, choose sturdy bud, cut with scalpel；
(2) tender shoots cut being rinsed disinfection, the stem apex for obtaining 0.3-1.0mm in an aseptic environment is explant,
It is pressed rapidly and is cultivated 30-40 days in sterile working program access MS culture medium, ginger budlet can be obtained, these budlets are continued
Culture 40-50 days, can be obtained complete ginger seedling；
(3) matrix for being suitble to the good air permeability of ginger growth is configured, puts into substrate pot, then fills matrix, it can not
With hand pressing matrix and vibration substrate pot, basin suffers basin and closely discharges, sufficiently sprinkled profoundly water with spray head it is spare, and cannot be with spray head just
It being sprayed against matrix, Ying Rangshui is fallen naturally at 45 DEG C of parabola, in case matrix is hardened；The detoxification ginger that step (2) is obtained
Seedling is inserted perpendicularly into matrix, and cuttage depth is 1cm or so, and gently squeezes matrix with thumb and index finger, keeps seedling abundant with matrix
In conjunction with, while reasonable cutting density is controlled, it sprinkles profoundly water, is put a lodged plant upright in time after watering immediately after the completion, make the upright growth of seedling,
Transparent plastic film is covered immediately after having poured water, and film surrounding compacting pressure is tight, to ensure that humidity is maximum in film；
(4) shading treatment is carried out with sunshade net before cuttage, first after cuttage week is protected from light daytime completely, can be appropriate after 1 week
The light for increasing early morning after the dusk, is kept for be aerated in the morning, it is not tight that ventilation while notices whether film covers daily；Cuttage
The 10-14 days afterwards, cuttings root system was grown, and can be removed the thin carry out transition of film sooner or later, can be removed film completely after transition 7 days,
It after film removes, should be sprinkled profoundly water immediately with big spray head, the 2nd day after watering, spray 1000 times of liquid of Bravo, entered later normal
Management periodically carries out fertilising and the prevention and treatment of pest and disease damage appropriate after seedling is taken root completely.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, step are placed in artificial light described in (1)
According to being cultivated in incubator, refer in 40 DEG C of temperature of setting, humidity 70%, under conditions of illumination is 0.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, disinfection described in step (2) are specific to grasp
As: it is rinsed 20-30 minutes with detergents such as washing powder, is transferred to superclean bench and is operated, after bud section is put into sterilizing
In beaker, impregnates 20 minutes with 75% alcohol washes 30 seconds, aseptic water washing 1 time, then with 0.1% mercuric chloride solution, finally use
The ginger bud section for completing sterilization operation is placed on suck dry moisture on aseptic filter paper by aseptic water washing 5-6 times.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, matrix described in step (3), specially
Peat, coco bran, perlite are mixed according to the ratio of volume ratio 3: 1: 1, and then stirring crushes, and crosses 60 meshes.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred ensures described in step (3) wet in film
Degree is maximum, concrete operations are as follows: meets high temperature or strong sunlight, need to shade or spraying measure cools down and increase air humidity, control wet
Spend 75-80%.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, it is complete to seedling described in step (4)
After taking root, fertilising and the prevention and treatment of pest and disease damage appropriate are periodically carried out, specially applied disposable fertilizer every 10-15 days, while every
Spray within 15-20 days the broad spectrum antimicrobial agents such as Bravo, carbendazim or the Mancozeb of 1000 times of liquid.
The beneficial effects of the present invention are:
The invention firstly uses the methods that heat treatment, Shoot Tip Culture and chemical agent combine to carry out detoxicated ginger, then
Culture medium culture is carried out using the ginger bud after detoxification, it can be achieved that the proliferation of ginger test tube seedling, taking root and a step culture of strong sprout；
Ginger seedling is subjected to cutting propagation using straight cutting method with plastic film after cultivation, by rationally controlling environment tune after cuttage pre-treatment, cuttage
Control, water and fertilizer management etc., compared with normal cutting propagation, straight cutting overlay film is able to maintain soil moisture and air humidity, inhibits weeds, promotes kind
Seedling root system is developed in advance, and rooting rate significantly improves, and number of days of taking root shortens 3-5 days, and seedling growing way and quality are also improved, simultaneously
It eliminates and builds Small plastic shed, carries out repeatedly the management works such as spraying daily, improve labor efficiency, relative reduction seedling cost,
It increases economic efficiency.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained, and however, the following embodiments are merely preferred embodiments of the present invention, and not all.
Based on the implementation example in the implementation mode, those skilled in the art's obtained other realities without making creative work
Example is applied, protection scope of the present invention is belonged to.Experimental method in following embodiments is unless otherwise specified conventional method,
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, comprising the following specific steps
(1) yellow ginger is placed in clean sand table, covers the fine sand of about 3 ㎝ thickness above, water spray keeps river sand wet, with sand table
Inside ponding is not advisable, and is covered on river sand with wet towel, is placed in artificial lighting incubator (40 DEG C of temperature of setting, humidity 70%, illumination
For culture in 0), is taken out when bud is grown to 1-2 ㎝ after 4-6 week, choose sturdy bud, cut with scalpel；
(2) tender shoots cut being rinsed disinfection, the stem apex for obtaining 0.3-1.0mm in an aseptic environment is explant,
It is pressed rapidly and is cultivated 30-40 days in sterile working program access MS culture medium, ginger budlet can be obtained, these budlets are continued
Culture 40-50 days, can be obtained complete ginger seedling；
(3) matrix for being suitble to the good air permeability of ginger growth is configured, puts into substrate pot, then fills matrix, it can not
With hand pressing matrix and vibration substrate pot, basin suffers basin and closely discharges, sufficiently sprinkled profoundly water with spray head it is spare, and cannot be with spray head just
It being sprayed against matrix, Ying Rangshui is fallen naturally at 45 DEG C of parabola, in case matrix is hardened；The detoxification ginger that step (2) is obtained
Seedling is inserted perpendicularly into matrix, and cuttage depth is 1cm or so, and gently squeezes matrix with thumb and index finger, keeps seedling abundant with matrix
In conjunction with, while reasonable cutting density is controlled, it sprinkles profoundly water, is put a lodged plant upright in time after watering immediately after the completion, make the upright growth of seedling,
Transparent plastic film is covered immediately after having poured water, and film surrounding compacting pressure is tight, to ensure that humidity is maximum in film, meets high temperature or sun
Light is strong, need to shade or spraying measure cools down and increase air humidity, control humidity 75-80%；
(4) shading treatment is carried out with sunshade net before cuttage, first after cuttage week is protected from light daytime completely, can be appropriate after 1 week
The light for increasing early morning after the dusk, is kept for be aerated in the morning, it is not tight that ventilation while notices whether film covers daily；Cuttage
The 10-14 days afterwards, cuttings root system was grown, and can be removed the thin carry out transition of film sooner or later, can be removed film completely after transition 7 days,
It after film removes, should be sprinkled profoundly water immediately with big spray head, the 2nd day after watering, spray 1000 times of liquid of Bravo, entered later normal
Management periodically carries out fertilising and the prevention and treatment of pest and disease damage appropriate, applied disposable fertilizer every 10-15 days after seedling is taken root completely,
The broad spectrum antimicrobial agents such as Bravo, carbendazim or the Mancozeb of 1000 times of liquid were sprayed every 15-20 days simultaneously.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, disinfection described in step (2) are specific to grasp
As: it is rinsed 20-30 minutes with detergents such as washing powder, is transferred to superclean bench and is operated, after bud section is put into sterilizing
In beaker, impregnates 20 minutes with 75% alcohol washes 30 seconds, aseptic water washing 1 time, then with 0.1% mercuric chloride solution, finally use
The ginger bud section for completing sterilization operation is placed on suck dry moisture on aseptic filter paper by aseptic water washing 5-6 times.
A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, matrix described in step (3), specially
Peat, coco bran, perlite are mixed according to the ratio of volume ratio 3: 1: 1, and then stirring crushes, and crosses 60 meshes.
The basic principles, main features and advantages of the present invention have been shown and described above.The technology of the industry
For personnel it should be appreciated that the present invention is not limited to the above embodiments, described in the above embodiment and specification is only the present invention
Preference, be not intended to limit the invention, without departing from the spirit and scope of the present invention, the present invention also has various
Changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by institute
Attached claims and its equivalent thereof.
Claims (6)
1. a kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred, which is characterized in that including walking in detail below
It is rapid:
(1) yellow ginger is placed in clean sand table, covers the fine sand of about 3 ㎝ thickness above, water spray keeps river sand wet, in sand table not
Ponding is advisable, and is covered on river sand with wet towel, is placed in culture in artificial lighting incubator, takes when bud is grown to 1-2 ㎝ after 4-6 week
Out, sturdy bud is chosen, is cut with scalpel；
(2) tender shoots cut is rinsed disinfection, the stem apex for obtaining 0.3-1.0mm in an aseptic environment is explant, by it
Rapidly by culture 30-40 days in sterile working program access MS culture medium, ginger budlet can be obtained, these budlets are continued to cultivate
40-50 days, complete ginger seedling can be obtained；
(3) matrix for being suitble to the good air permeability of ginger growth is configured, puts into substrate pot, then fills matrix, unavailable hand
Matrix and vibration substrate pot are pressed, basin suffers basin and closely discharge, and is sufficiently sprinkled profoundly water with spray head spare, and cannot be faced with spray head
Matrix spray, Ying Rangshui is fallen naturally at 45 DEG C of parabola, in case matrix is hardened；The detoxification ginger seedling that step (2) is obtained hangs down
Straight cutting enters matrix, and cuttage depth is 1cm or so, and gently squeezes matrix with thumb and index finger, combines seedling sufficiently with matrix,
Reasonable cutting density is controlled simultaneously, sprinkles profoundly water, is put a lodged plant upright in time after watering immediately after the completion, make the upright growth of seedling, poured
Transparent plastic film is covered after water immediately, film surrounding compacting pressure is tight, to ensure that humidity is maximum in film；
(4) shading treatment is carried out with sunshade net before cuttage, first after cuttage week is protected from light daytime completely, can suitably increase after 1 week
The light in early morning after dusk is kept for be aerated in the morning daily, and it is not tight that ventilation while notices whether film covers；After cuttage
10-14 days, cuttings root system was grown, and can be removed the thin carry out transition of film sooner or later, can be removed film, film completely after transition 7 days
It after removing, should be sprinkled profoundly water immediately with big spray head, the 2nd day after watering, spray 1000 times of liquid of Bravo, enter normal pipe later
Reason periodically carries out fertilising and the prevention and treatment of pest and disease damage appropriate after seedling is taken root completely.
2. the method that the self tissue culture bud straight cutting overlay film of a kind of detoxification ginger according to claim 1 is bred, feature
It is, culture in artificial lighting incubator is placed in described in step (1), refers to that, in 40 DEG C of temperature of setting, humidity 70%, illumination is
Under conditions of 0.
3. the method that the self tissue culture bud straight cutting overlay film of a kind of detoxification ginger according to claim 1 is bred, feature
It is, disinfection described in step (2), concrete operations are as follows: rinsed 20-30 minutes with detergents such as washing powder, be transferred to ultra-clean work
Platform is operated, and bud section is put into the beaker after sterilizing, with 75% alcohol washes 30 seconds, aseptic water washing 1 time, then is used
0.1% mercuric chloride solution impregnates 20 minutes, finally uses aseptic water washing 5-6 times, will complete the ginger bud section of sterilization operation
It is placed on suck dry moisture on aseptic filter paper.
4. the method that the self tissue culture bud straight cutting overlay film of a kind of detoxification ginger according to claim 1 is bred, feature
It is, matrix described in step (3), specially peat, coco bran, perlite are mixed according to the ratio of volume ratio 3: 1: 1, are then stirred
Breading is broken, crosses 60 meshes.
5. the method that the self tissue culture bud straight cutting overlay film of a kind of detoxification ginger according to claim 1 is bred, feature
It is, ensures that humidity is maximum in film described in step (3), concrete operations are as follows: meet high temperature or strong sunlight, need to shade or arrange by spraying
It applies cooling and increases air humidity, control humidity 75-80%.
6. the method that the self tissue culture bud straight cutting overlay film of a kind of detoxification ginger according to claim 1 is bred, feature
It is, described in step (4) after seedling is taken root completely, periodically carries out fertilising and the prevention and treatment of pest and disease damage appropriate, specially
Disposable fertilizer was applied every 10-15 days, while Bravo, carbendazim or the Mancozeb of 1000 times of liquid were sprayed every 15-20 days
Equal broad spectrum antimicrobial agents.
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| CN201810592556.2A CN109042317A (en) | 2018-06-11 | 2018-06-11 | A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810592556.2A CN109042317A (en) | 2018-06-11 | 2018-06-11 | A kind of method that the self tissue culture bud straight cutting overlay film of detoxification ginger is bred |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110896838A (en) * | 2019-12-06 | 2020-03-24 | 神州姜窖农业集团有限公司 | Soilless culture method for detoxified ginger seedlings |
| CN111837959A (en) * | 2020-08-05 | 2020-10-30 | 四川农业大学 | Micro ginger block induction method based on ginger test-tube plantlet and application |
| CN111869444A (en) * | 2020-07-23 | 2020-11-03 | 广西壮族自治区农业科学院 | Rescue breeding method for jasmine |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393742A (en) * | 2015-10-21 | 2016-03-16 | 夹江县宏博果蔬种植专业合作社 | Open field ginger large-area seed propagation cultivation method |
| CN107810857A (en) * | 2017-12-22 | 2018-03-20 | 济南亚龙生物科技有限公司 | A kind of detoxicated ginger method for tissue culture |
-
2018
- 2018-06-11 CN CN201810592556.2A patent/CN109042317A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393742A (en) * | 2015-10-21 | 2016-03-16 | 夹江县宏博果蔬种植专业合作社 | Open field ginger large-area seed propagation cultivation method |
| CN107810857A (en) * | 2017-12-22 | 2018-03-20 | 济南亚龙生物科技有限公司 | A kind of detoxicated ginger method for tissue culture |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110896838A (en) * | 2019-12-06 | 2020-03-24 | 神州姜窖农业集团有限公司 | Soilless culture method for detoxified ginger seedlings |
| CN111869444A (en) * | 2020-07-23 | 2020-11-03 | 广西壮族自治区农业科学院 | Rescue breeding method for jasmine |
| CN111869444B (en) * | 2020-07-23 | 2022-04-12 | 广西壮族自治区农业科学院 | Rescue breeding method for jasmine |
| CN111837959A (en) * | 2020-08-05 | 2020-10-30 | 四川农业大学 | Micro ginger block induction method based on ginger test-tube plantlet and application |
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Application publication date: 20181221 |