CN107047259A - A kind of method of bulb block air culture cutting propagation Lycoris radiata - Google Patents
A kind of method of bulb block air culture cutting propagation Lycoris radiata Download PDFInfo
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- CN107047259A CN107047259A CN201710267686.4A CN201710267686A CN107047259A CN 107047259 A CN107047259 A CN 107047259A CN 201710267686 A CN201710267686 A CN 201710267686A CN 107047259 A CN107047259 A CN 107047259A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
The invention discloses a kind of method of bulb block air culture cutting propagation Lycoris radiata, including：1）Using " cross " quartering cutting size, impartial, the Lycoris radiata bulb of growing way health, obtains bulb block；2）By bulb block with 500 mg/L IBA solution soak 20s, then with 4 g/L carbendazim solutions soak 30 min disinfect afterwards dry in the shade naturally；3）Using air culture method rapid induction bulb block axillary bud and root growth, the cuttage culture into matrix is transplanted after certain time, young ball is obtained.This method sets up clonal regeneration system using Lycoris radiata mother bulb, rapid induction axillary bud development is realized with reference to air culture, proliferation rate is up to 720%, and obtained young ball has hereditary basis consistent, character is stable, growth potential is neat, and more bulb can be obtained in a short time, substantial amounts of raw material are provided for industries such as fancy horticulture, pharmacy.
Description
Technical field
The invention belongs to short-tube lycoris Cultivating techniques field, it is related to a kind of method of bulb block air culture cutting propagation Lycoris radiata,
The growth for accelerating Lycoris radiata bulb block axillary bud and root system is induced in particular with air culture method.
Background technology
Lycoris radiata (Lycoris radiate) is Amaryllidaceae Lycoris herbaceos perennial, and there is spherical squama underground
Stem, outsourcing crineous film quality squamation.Its flowers is peculiar gorgeous, graceful, with high ornamental value, can make cut-flower, basin
Flower and sight flower see leaf cover plant.Its bulb contains lycorine (Lycorine), galanthamine (Galanthamine) etc. more than 10
Alkaloid and important medical components are planted, are widely used in medical science, bio-pharmaceuticals.In addition, also containing abundant starch in its bulb
And carbohydrate gum, available for processing alcohol and paper grade (stock) thickener and senior paste.Some polysaccharide, amino acid and trace element, again
With higher healthy nutritive value.These current compositions much can be with commercial size extraction and application.
But, Lycoris radiata is generally triploid, mainly based on vegetative propagation, and annual mother's bulb is with " dividing naturally " method
It is split into a daughter bulb and grows into a new plant, natural propagation coefficient is very low.In recent years, because a large amount of of pharmaceuticals industry carry
Take, the original distribution most most Lycoris radiata of wide, reserves and the wild resource of Lycoris aurea are also drastically reduced.Therefore, bulb is solved
Fast numerous and cultivation technique be to make full use of Lycoris resource, form the premise and key of industrialization development and large-scale production.
At present, cutting propagation and tissue cultures are two kinds of numerous approach of expansion of most study.Though tissue cultures can quickly be bred, technology
It is required that it is higher with cost, and the bulb bred reaches that requirement required time of blooming is longer.Therefore use cuttage breeding method to carry out
It is method the most cost-effective to expand numerous.Air culture method is especially combined, rapid induction bulb block axillary bud, root growth is realized, led to
Cross plateau cutting to be multiplied young ball quantity, greatly improve proliferation rate.
The content of the invention
Goal of the invention：For the deficiencies in the prior art, it is an object of the invention to provide a kind of bulb block air culture skewer
Insert the method for breeding Lycoris radiata, breeding Lycoris radiata that can be rapidly and efficiently.
Technical scheme：In order to realize foregoing invention purpose, the technical solution adopted by the present invention is：
A kind of method of bulb block air culture cutting propagation Lycoris radiata, comprises the following steps：
1) perennial, the Lycoris radiata bulb that size is basically identical, growing way is healthy through artificial cultivation are selected, is removed residual
Leaf, cut off root system, remove bulb brown epithelium, clean up, dry；
2) the Lycoris radiata bulb picked out is cut using " cross " quartering, bulb block is obtained；With IBA solution and
After carbendazim solution sterilization, dry in the shade；
3) the bulb block after sterilization is positioned on the plateau after sterilization, spacing 2-3 ㎝ between each bulb block；Put
After the completion of putting, coating gauze above bulb block；Periodically watering, sterilization, after air culture room is cultivated 8-12 days, root growth 3-6
㎝；
4) the bulb block after Rooting by Air-culture is uniformly dipped into appropriate plant ash, be inserted directly into cutting medium, micro- dew top
End, controls water content of substrate 10~20% before cuttage；After cuttage is finished, pour permeable；
5) matrix keeps 55~65% water content；Increase spray timings, rainy season timely draining daily when temperature is higher；
The weeds grown on slotting machine are pulled out in time, pour matrix once with carbendazim solution weekly；Culture extremely obtains young ball.
Step 1) in, Lycoris radiata bulb, average week footpath 4-5cm, mean fresh 35-50g, average scale number of plies 10-15
Layer.
Step 2) in, first by bulb top l/3 Partial Resection, remaining part is centered on bulb basal disc, according to " ten "
Shape is averagely cut into 4 deciles, obtains bulb block.
Step 2) in, bulb block is first soaked in more than 100~500mg/L IBA solution disinfections 20s, then by bulb
Block is immersed in more than 4g/L carbendazim solutions 30min sterilizations.
Step 3) in, sterilize plastic pallet, it is desirable to which tray bottom and side wall are designed with through hole, can be fully ventilated, support
Tray bottom lays 3 layers of sterilizing newspaper and 2 layers of sterile absorbent cotton.
Step 3) in, daily morning and evening same time watering can uniform sprinkling is all moistened by newspaper and gauze and is defined, weekly
Squirted once with 1000 times of carbendazim solutions.
Step 3) in, temperature control keeps good ventilated environment at 26 ± 1 DEG C, and air culture room is cultivated 8-12 days
Afterwards, root growth 3-6 ㎝ length, the scale interlayer of bulb block starts l-2 axillary bud occur, and female bulb-scale starts turnup.
Step 4) in, cuttage bedside 0.6m, long 0.8m, high 0.2m, bed is interior to be laid on the thick cutting mediums of 15 ㎝, slotting machine
Fang Shidu sunshades.
Step 4) in, it is peat from cutting medium：Perlite：Vermiculite：Sand=1：1：1：1~2, matrix is used in advance
5% carbendazim solution is sterilized, and holding drying is dried after sterilization.
Beneficial effect：Compared with prior art, the method for bulb block air culture cutting propagation Lycoris radiata of the invention, profit
Set up clonal regeneration system with Lycoris radiata mother bulb, rapid induction axillary bud development realized with reference to air culture, proliferation rate up to 720%,
And obtained young ball has hereditary basis consistent, character is stable, and growth potential is neat, and more bulb can be obtained in a short time,
Substantial amounts of raw material are provided for industries such as fancy horticulture, pharmacy.
Brief description of the drawings
Fig. 1 is that Lycoris radiata bulb block is obtained and result figure；
Fig. 2 is Lycoris radiata bulb air culture setting and axillary bud growth situation map；
Fig. 3 is bulb root tuber system, young ball growing state figure after air culture；
Fig. 4 is cuttage growing state figure；
Fig. 5 is bulb root tuber system, young ball growing state figure.
Embodiment
With reference to specific embodiment, the present invention is described further.
Embodiment 1
The method of the bulb block air culture cutting propagation Lycoris radiata of the present invention, step is as follows：
1st, the preparation of bulb, the acquisition of bulb block
1) bulb is handled：Select perennial, the Lycoris radiata kind that size is basically identical, growing way is healthy through artificial cultivation
Ball, average week footpath 4.5cm, mean fresh 45g, average 14 layers of the scale number of plies.Residual leaf is removed, root system (not hindering basal disc) is cut off, goes
Except bulb brown epithelium, clean up, dry.
2) patterning method obtains bulb block：The Lycoris radiata bulb picked out is cut using " cross " quartering, first by squama
Stem top l/3 Partial Resection, remaining part is averagely cut into 4 deciles centered on bulb basal disc according to " ten " shape, obtains squama
Stem block.
3) processing of bulb block auxin and sterilization：500mg/L IBA solution is configured, and bulb block is soaked in the solution
20s.4g/L carbendazim solutions are configured afterwards, and bulb block is soaked to take out after 30min and dried in the shade.
Lycoris radiata bulb block is obtained and result, as shown in Figure 1.
2nd, air culture method is taken root
1) air culture environment is built：Sterilize plastic pallet, it is desirable to which tray bottom and side wall are designed with through hole, can fully divulge information
Ventilative, 3 layers of sterilizing newspaper and 2 layers of sterile absorbent cotton are laid in tray bottom.Plastic pallet is placed in the high clean experimental benches of 1.5m
On.
2) air culture is managed：Bulb block after sterilization is positioned on plateau, spacing 2-3 ㎝ between each bulb block；Put
After the completion of putting, 2 layers of gauze are covered above bulb block.Daily morning and evening same time watering can uniform sprinkling, with newspaper and gauze all
Moistening is defined, and is squirted once with 1000 times of carbendazim solutions weekly.Temperature control is at 26 DEG C or so, and the good ventilation of holding is saturating
Compression ring border, after air culture room is cultivated 12 days, as shown in Fig. 2 4.8 ㎝ length of root growth, the scale interlayer of bulb block starts
Existing l-2 axillary bud, female bulb-scale starts turnup, now prepares to transplant and is cultivated into matrix.
3) cutting propagation
1) preparation of cutting medium：Cuttage thick 15 ㎝ of laying in cuttage bedside 0.6m, long 0.8m, high 0.2m, bed is set
Appropriate sunshade above matrix, slotting machine.It is peat from cutting medium：Perlite：Vermiculite：Sand=1：1：1：2.Matrix shifts to an earlier date
Sterilized with 5% carbendazim solution, holding drying is dried after sterilization.
2) cuttage of bulb block：Bulb block after Rooting by Air-culture is uniformly dipped into appropriate plant ash, cuttage base is inserted directly into
In matter, cuttage depth about 3cm, micro- dew top controls water content of substrate 15% or so before cuttage.
3) cuttage is managed：After cuttage is finished, pour permeable.This backsight weather conditions manually spray benefit it is wet, keep matrix
60% or so water content；Increase spray timings, rainy season timely draining daily when temperature is higher；The weeds that are grown on slotting machine and
When pull out.Weekly matrix is poured with 1000 times of carbendazim solutions once.
Young ball growing state is as shown in figure 3, cuttage growing state is as shown in Figure 4.From institute into from the point of view of bulbec quantity situation,
Generally each bulb block produces 1-2 new bulbs, and proliferation rate is 740%, and the calculation formula of proliferation rate is：After Lycoris radiata bulb block air culture 8d,
Now just there is a new root generation, rooting rate is up to 90%.New root is generally 1-2 bars.After 10d, new root length is of about 4-10 ㎝.Afterwards, root
It is that length has still increased, quantity increased.After 12d, regeneration clove is grown by the basal disc of bulb block internal layer, and diameter is
Up to 1-2cm, color and luster is delicate, no epithelium coating.After 15d, part bulb block 1-2 bead of formation.During 20d, clove diameter reaches
To 2-3cm, root system has become flourishing.During 30d, clove diameter reaches 3-4cm.During 50d, clove diameter reaches 3-4cm.Therefore
In the case where female bulb is in the same size, cutter number is more, and bulbec quantity is more.Therefore, breeding can select big as far as possible
Mother bulb preferably to cut several knives to an equal amount of mother bulb more.Because Lycoris bulb contains substantial amounts of moisture, general water content in itself
>70%, if so cutting medium soil moisture is unsuitable too high, easily generation is rotted, so the present embodiment uses matrix mud
Charcoal：Perlite：Vermiculite：Sand=1：1：1：2 cutting experiments are preferable, and can be significantly by seed soaking and seed dressing sterilization after cutting
Reduce and rot.Bulb block root growth can be accelerated by carrying out Lycoris radiata cuttage by air culture method, and bud ratio is high and stably, first
Cause bulb root tuber system to grow into certain length and quantity using air culture method culture, transfer to cuttage in suitable matrix and train
Support, can so accelerate bulb proliferative speed, improve young ball quality.
Embodiment 2
The method of the bulb block air culture cutting propagation Lycoris radiata of the present invention, step is as follows：
1st, the preparation of bulb, the acquisition of bulb block
1) bulb is handled：Select perennial, the Lycoris radiata kind that size is basically identical, growing way is healthy through artificial cultivation
Ball, average week footpath 4cm, mean fresh 38g, average 13 layers of the scale number of plies.Remove residual leaf, cut off root system (not hindering basal disc), removal
Bulb brown epithelium, cleans up, dries.
2) patterning method obtains bulb block：The Lycoris radiata bulb picked out is cut using " cross " quartering, first by squama
Stem top l/3 Partial Resection, remaining part is averagely cut into 4 deciles centered on bulb basal disc according to " ten " shape, obtains squama
Stem block.
3) processing of bulb block auxin and sterilization：100mg/L IBA solution is configured, and bulb block is soaked in the solution
6h.4g/L carbendazim solutions are configured afterwards, and bulb block is soaked to take out after 30min and dried in the shade.
2nd, air culture method is taken root
1) air culture environment is built：Sterilize plastic pallet, it is desirable to which tray bottom and side wall are designed with through hole, can fully divulge information
Ventilative, 3 layers of sterilizing newspaper and 2 layers of sterile absorbent cotton are laid in tray bottom.Plastic pallet is placed in the high clean experimental benches of 1.5m
On.
2) air culture is managed：Bulb block after sterilization is positioned on plateau, spacing 2-3 ㎝ between each bulb block；Put
After the completion of putting, 2 layers of gauze are covered above bulb block.Daily morning and evening same time watering can uniform sprinkling, with newspaper and gauze all
Moistening is defined, and is squirted once with 1000 times of carbendazim solutions weekly.Temperature control keeps good at 26 DEG C or so in laboratory
Ventilated environment, after air culture room is cultivated 12 days, 4.8 ㎝ length of root growth, the scale interlayer of bulb block starts to occur
L-2 axillary bud, female bulb-scale starts turnup, now prepares to transplant and is cultivated into matrix.
3rd, cutting propagation
1) preparation of cutting medium：Cuttage thick 15 ㎝ of laying in cuttage bedside 0.6m, long 0.8m, high 0.2m, bed is set
Appropriate sunshade above matrix, slotting machine.It is peat from cutting medium：Vermiculite：Sand=1：1： 1.Matrix is in advance with bacterium more than 5%
Holding drying is dried after clever solution disinfection, sterilization.
2) cuttage of bulb block：Bulb block after Rooting by Air-culture is uniformly dipped into appropriate plant ash, cuttage base is inserted directly into
In matter, cuttage depth about 3cm, micro- dew top controls water content of substrate 15% or so before cuttage.
3) cuttage is managed：After cuttage is finished, pour permeable.This backsight weather conditions manually spray benefit it is wet, keep matrix
60% or so water content；Increase spray timings, rainy season timely draining daily when temperature is higher；The weeds that are grown on slotting machine and
When pull out.Weekly matrix is poured with 1000 times of carbendazim solutions once.
As a result show, from institute into from the point of view of young ball quantity situation, generally each bulb block produces 1-2 new bulbs, proliferation rate
For 700%.In addition, cutting technique has considerable influence to result of the test, wherein influence it is maximum for depth of cut, cutting is too
The shallow complete formation and growth for being unfavorable for young ball, preferably uniformly cutting more than 2/3 through bulb neck during cutting from substrate is
It is good.From matrix peat：Vermiculite：Sand=1：1：1 Lycoris radiata four finds the matrix divided by the cutting experiment of bulb block
Cutting experiment is less desirable, and trace it to its cause the matrix water conservation, retention ability are stronger, poor air permeability, cause bulb block pollution rate compared with
Height, be not suitable for bulb cuttage, particularly July it is hot and humid in the case of.By the IBA of various concentrations to scale at
Reason it was found that, 500mg/L IBA for short-tube lycoris scale cuttage reproductive effect preferably, 100mg/L IBA treatment effects take second place, it
Can promote Lycoris radiata cuttage root growth and axillary bud sprouting to a certain degree, improve short-tube lycoris scale life seed ball rate.
Embodiment 3
1st, the preparation of bulb, the acquisition of bulb block
1) bulb is handled：Select perennial, the Lycoris radiata kind that size is basically identical, growing way is healthy through artificial cultivation
Ball, average week footpath 4cm, mean fresh 38.6g, average 13 layers of the scale number of plies.Residual leaf is removed, root system (not hindering basal disc) is cut off, goes
Except bulb brown epithelium, clean up, dry.
2) patterning method obtains bulb block：The Lycoris radiata bulb picked out is cut using " cross " quartering, first by squama
Stem top l/3 Partial Resection, remaining part is averagely cut into 4 deciles centered on bulb basal disc according to " ten " shape, obtains squama
Stem block.
3) processing of bulb block auxin and sterilization：500mg/L IBA solution is configured, and bulb block is soaked in the solution
20s.4g/L carbendazim solutions are configured afterwards, and bulb block is soaked to take out after 30min and dried in the shade.
2nd, matrix cutting propagation
1) preparation of cutting medium：Cuttage thick 15 ㎝ of laying in cuttage bedside 0.6m, long 0.8m, high 0.2m, bed is set
Appropriate sunshade above matrix, slotting machine.It is peat from cutting medium：Perlite：Vermiculite：Sand=1：1：1：2.Matrix shifts to an earlier date
Sterilized with 5% carbendazim solution, holding drying is dried after sterilization.
2) cuttage of bulb block：Bulb block after Rooting by Air-culture is uniformly dipped into appropriate plant ash, cuttage base is inserted directly into
In matter, cuttage depth about 3cm, micro- dew top controls water content of substrate 15% or so before cuttage.
3) cuttage is managed：After cuttage is finished, pour permeable.This backsight weather conditions manually spray benefit it is wet, keep matrix
60% or so water content；Increase spray timings, rainy season timely draining daily when temperature is higher；The weeds that are grown on slotting machine and
When pull out.Weekly matrix is poured with 1000 times of carbendazim solutions once.
Bulb root tuber system, young ball growing state are as shown in Figure 5.As a result show, from institute into from the point of view of young ball quantity situation, lead to
Often each bulb block produces 1-2 new bulbs, and proliferation rate is 680%.After Lycoris radiata bulb block cuttage 15d, original scale is several
Whole brownings, now new root produce, rooting rate is up to 80%.New root is generally 1-2 bars, occasionally there is 3.After 30d, new root length reaches
About 4-10 ㎝.Afterwards, root length has still increased, quantity increased.After 30d, regeneration clove is by bulb block internal layer
Basal disc is grown, and diameter reaches 1-2cm, and color and luster is delicate, no epithelium coating.After 45d, part bulb block 1-2 bead of formation.60d
When, clove diameter reaches 2-3cm, and root system has become flourishing, grown slow.Under having compared to embodiment 1 and the proliferation rate of embodiment 2
Drop.The most it is evident that not setting up air culture process, Lycoris radiata bulb block root growth sprouts slower, and bud ratio is not
It is stable, until forming a certain size young ball needs the longer time.
Claims (9)
1. a kind of method of bulb block air culture cutting propagation Lycoris radiata, it is characterised in that comprise the following steps：
1）Perennial, the Lycoris radiata bulb that size is basically identical, growing way is healthy through artificial cultivation are selected, residual leaf is removed, cuts
Remove root system, remove bulb brown epithelium, clean up, dry；
2）The Lycoris radiata bulb picked out is cut using " cross " quartering, bulb block is obtained；With IBA solution and carbendazim
After solution disinfection, dry in the shade；
3）Bulb block after sterilization is positioned on the plateau after sterilization, spacing 2-3 ㎝ between each bulb block；Place and complete
Afterwards, coating gauze above bulb block；Periodically watering, sterilization, after air culture room is cultivated 8-12 days, root growth 3-6 ㎝；
4）Bulb block after Rooting by Air-culture is uniformly dipped into appropriate plant ash, is inserted directly into cutting medium, micro- dew top, cuttage
Preceding control water content of substrate is 10 ~ 20%；After cuttage is finished, pour permeable；
5）Matrix keeps 55 ~ 65% water content；Increase spray timings, rainy season timely draining daily when temperature is higher；It is long on slotting machine
The weeds gone out are pulled out in time, pour matrix once with carbendazim solution weekly；Culture extremely obtains young ball.
2. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 1）
In, Lycoris radiata bulb, average week footpath 4-5 cm, mean fresh 35-50g, average 10-15 layers of the scale number of plies.
3. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 2）
In, first by bulb top l/3 Partial Resection, remaining part is averagely cut into 4 etc. centered on bulb basal disc according to " ten " shape
Point, obtain bulb block.
4. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 2）
In, first bulb block is soaked in more than 100 ~ 500 mg/L IBA solution disinfections 20s, it is more that bulb block then is immersed in into 4 g/L
It is more than bacterium spirit solution 30 min sterilizations.
5. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 3）
In, sterilize plastic pallet, it is desirable to which tray bottom and side wall are designed with through hole, can be fully ventilated, tray bottom is laid 3 layers and gone out
Bacterium newspaper and 2 layers of sterile absorbent cotton.
6. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 3）
In, daily morning and evening same time watering can uniform sprinkling is all moistened by newspaper and gauze and is defined, molten with 1000 times of carbendazim weekly
Liquid is squirted once.
7. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 3）
In, temperature control is at 26 ± 1 DEG C, the good ventilated environment of holding, after air culture room is cultivated 8-12 days, root growth 3-6 ㎝
Length, the scale interlayer of bulb block starts l-2 axillary bud occur, and female bulb-scale starts turnup.
8. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 4）
In, cuttage bedside 0.6m, long 0.8m, high 0.2m, cutting medium thick 15 ㎝ of the interior laying of bed, the appropriate sunshade in slotting machine top.
9. the method for bulb block air culture cutting propagation Lycoris radiata according to claim 1, it is characterised in that step 4）
In, it is peat from cutting medium：Perlite：Vermiculite：Sand=1：1：1：1 ~ 2, matrix is sterilized with 5% carbendazim solution in advance,
Holding drying is dried after sterilization.
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| CN109329060A (en) * | 2018-11-21 | 2019-02-15 | 江苏省中国科学院植物研究所 | The method for carrying out tissue-culturing rapid propagation as explant to change brocade flower short-tube lycoris plateau |
| CN111109081A (en) * | 2020-01-03 | 2020-05-08 | 上海市农业科学院 | Lycoris radiata rootless tissue culture method and lycoris radiata cultivation method |
| CN111264204A (en) * | 2020-04-16 | 2020-06-12 | 江苏省中国科学院植物研究所 | Method for cutting and rapidly propagating straw lycoris radiata bulbs |
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| CN109329060A (en) * | 2018-11-21 | 2019-02-15 | 江苏省中国科学院植物研究所 | The method for carrying out tissue-culturing rapid propagation as explant to change brocade flower short-tube lycoris plateau |
| CN111109081A (en) * | 2020-01-03 | 2020-05-08 | 上海市农业科学院 | Lycoris radiata rootless tissue culture method and lycoris radiata cultivation method |
| CN111109081B (en) * | 2020-01-03 | 2022-03-22 | 上海市农业科学院 | Lycoris radiata rootless tissue culture method and lycoris radiata cultivation method |
| CN111264204A (en) * | 2020-04-16 | 2020-06-12 | 江苏省中国科学院植物研究所 | Method for cutting and rapidly propagating straw lycoris radiata bulbs |
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Application publication date: 20170818 |