CN105191769A - Cultivation method for increasing content of dendrobium officinale polysaccharides - Google Patents
Cultivation method for increasing content of dendrobium officinale polysaccharides Download PDFInfo
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- CN105191769A CN105191769A CN201510607293.4A CN201510607293A CN105191769A CN 105191769 A CN105191769 A CN 105191769A CN 201510607293 A CN201510607293 A CN 201510607293A CN 105191769 A CN105191769 A CN 105191769A
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- seedling
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention discloses a cultivation method for increasing the content of dendrobium officinale polysaccharides. The cultivation method includes the steps that tissue culture is performed on seedlings, the seedlings are subpackaged, the seedlings are transplanted, spraying watering is performed, temperature management is performed, topdressing management is performed, illumination management is performed, disease control is performed, and pest control is performed. The steps are simple and reasonable, the survival rate of the transplanted dendrobium officinale is high, growth speed is high, few plant diseases and insect pests occur, through performing control management on humidity, temperature and illumination inside a greenhouse, the day and night temperature difference inside the greenhouse is suitable while effective accumulated temperature is guaranteed, under sufficient illumination conditions, photosynthesis is facilitated, and conveying of nutritive substances to stems and accumulation of the nutritive substances are facilitated. Compared with the prior art, the content of the cultivated dendrobium officinale polysaccharides is remarkably increased, the technical problems that in the prior art, the content of dendrobium officinale polysaccharides is low, and medicinal value is low are solved, and the medicinal value of the dendrobium officinale is increased.
Description
[technical field]
The present invention relates to the technical field of officinal dendrobium stem plantation, particularly a kind of cultivation method improving polysaccharide content of dendrobium candidum.
[background technology]
Dendrobium candidum is a kind of famous and precious medicinal material, its cold nature, taste is sweet, light, there is effect that is nourishing Yin and clearing heat, nourishing the stomach to improve the production of body fluid, can be used for treating and there is the illness such as the acute of yin deficiency syndrome and pharyngo-laryngitis chronica, chronic bronchitis, tuberculosis, hyperthyroidism, diabetes, chronic hepatitis, atrophic gastritis, immunologic function syndrome and carcinosis radiotherapy and chemotherapy conditioning, and old resistance against diseases in can strengthening.
Dendrobium officinale polysaccharide belongs to the one of active polysaccharide, has multiple efficacies.The immunoregulation effect of human body is the acting in conjunction of most of active polysaccharide, modern pharmacology is researched and analysed and is shown, the emplastic mainly water-soluble polysaccharide of dendrobium candidum stem, content accounts for the ratio of its dry weight up to more than 25%, it is a para-immunity reinforcing agent, shifter factor is produced to raising peripheral leukocytes and promotion lymphocyte there is obvious effect, Dendrobium officinale polysaccharide composition has growth T, bone-marrow-derived lymphocyte, the remarkable effect of the immune cell functions such as macrophage and natural killer cell (NK), can effective Tumor suppression, Dendrobium officinale polysaccharide has hypoglycemic effect, long-term taking Western medicine is hypoglycemic, insulin injection has very large side effect, and the extraction of Dendrobium officinale polysaccharide pure natural plant, have no side effect, different from oral hypoglycemic sulfonylurea, it to euglycemia without reducing effect, also serum insulin level is not improved, and to islet cells, there is repair, also there is the outer hypoglycemic effect of pancreas simultaneously, the hepatic glycogenolytic caused by suppressing adrenaline and the synthesis of promotion hepatic glycogen, reach the object reducing blood glucose value, in addition, Dendrobium officinale polysaccharide anti-oxidant, anti-aging effects.
At present, in officinal dendrobium stem plantation technology, the growth rate of dendrobium candidum is very fast, and in dendrobium candidum, polyoses content is lower, reduces its medical value, is therefore necessary to propose a kind of cultivation method improving polysaccharide content of dendrobium candidum.
[summary of the invention]
The object of the invention is to overcome above-mentioned the deficiencies in the prior art, provide a kind of cultivation method improving polysaccharide content of dendrobium candidum, its polyoses content being intended to solve dendrobium candidum in prior art is lower, the technical problem that its medical value is not high.
For achieving the above object, the present invention proposes a kind of cultivation method improving polysaccharide content of dendrobium candidum, comprise the steps:
Step one, group training seedling: choose wild dendrobium candidum seedling, carry out explant cultivation, after turning out bud or leaf bud, bud or leaf bud are put into medium and carries out sterile propagation cultivation, cultivation temperature 25 DEG C ~ 28 DEG C, illumination every day 9 ~ 10 hours, intensity of illumination is 32.4 ~ 34.2 μm of ol.m
-2.s
-1;
Step 2, seedling packing: when growth of seedling is to about 2cm, is dispensed in blake bottle and cultivates, cultivate 4 ~ 6 strain seedling in each blake bottle;
Step 3, seedling replanting: when the seedling root length in blake bottle is 0.5 ~ 1cm, choosing 3 strain seedling is one clump, every strain seedling height of seedling 4 ~ 5cm, at least containing 5 blades, 5 corings, the medium of seedling root is first cleaned with clear water, use the potassium permanganate dilution spray disinfectant of 0.1% again, then with the line-spacing field planting of 20cm × 20cm in the matrix in booth, light irradiation apparatus, ventilation unit and humidity conditioner are set in booth;
Step 4, spraying are watered: water in the mode of spraying to the seedling just transplanted to booth, water to grabbing matrix with hand and have wet sense but do not drip and be advisable, transplant in one week, about 90% is remained on by the humidity that humidity conditioner controls in booth, after one week, the humidity controlled in booth remains on 70 ~ 80%, and adopts ventilation unit to ventilate;
Step 5, temperature treatment: the day temperature in booth controls at 25 ~ 30 DEG C, night temperatures controls at 18 ~ 20 DEG C, during summer high temperature, adopt the form cooling of spraying, and ventilation and heat, during winter low temperature, the coated sealing of booth double-plastic, prevents plant freeze injury;
Step 6, management of topdressing: seedling replanting, after two weeks, when seedling grows new root, makes foliage-spray by alga fertilizer, when treating that seedling is looked slightly a little bigger, carry out foliage spray with the general fertilizer of doctor Lu, the compound organic fertilizer material of root nutrient >=45% sprays, the amount of spraying of topdressing is few, within every two weeks, carries out once;
Step 7, illumination management: booth adopts the shade net of 70% to shelter from heat or light, and adopts light irradiation apparatus to carry out illumination management to plant, the intensity of illumination controlled in booth is 80 ~ 120 μm of ol.m
-2.s
-1;
Step 8, disease control: monthly spray with bordeaux mixture and carry out foliar control;
Step 9, pest control: booth outer cladding one deck fly net, carry out insect pest removal by the mode be manually combined with medicine to booth, prevents plant to wither.
As preferably, the matrix in described step 3 becomes bits by bark sawdust, and by boiling water 1 hour, cooling immersion 2 ~ 3 days naturally, then dries and form, and the filling thickness of booth mesostroma is greater than 8cm.
As preferably, the water pH value of watering of spraying in described step 4 controls between 4.5 ~ 5.5.
As preferably, described step 8 Chinese traditional medicine control can adopt equivalent formula bordeaux mixture 800 ~ 1000 times of liquid.
Beneficial effect of the present invention: compared with prior art, a kind of cultivation method improving polysaccharide content of dendrobium candidum provided by the invention, simple and reasonable steps, dendrobium candidum survival rate after transplanting is high, fast growth, and damage by disease and insect is few, by to the humidity in booth, controlization management is carried out in temperature and illumination, make the day and night temperature in booth more suitable while ensure that effective accumulated temperature, under the illumination condition of abundance, be conducive to photosynthesis, be conducive to nutriment to transport and accumulation to cane, improve the polyoses content in dendrobium candidum, thus improve the medical value of dendrobium candidum.
Feature of the present invention and advantage will be described in detail by embodiment.
[embodiment]
For making the object, technical solutions and advantages of the present invention clearly understand, below by embodiment, the present invention is further elaborated.But should be appreciated that, specific embodiment described herein, only in order to explain the present invention, is not limited to scope of the present invention.In addition, in the following description, the description to known features and technology is eliminated, to avoid unnecessarily obscuring concept of the present invention.
The embodiment of the present invention provides a kind of cultivation method improving polysaccharide content of dendrobium candidum, comprises the steps:
Step one, group training seedling: choose wild dendrobium candidum seedling, carry out explant cultivation, after turning out bud or leaf bud, bud or leaf bud are put into medium and carries out sterile propagation cultivation, cultivation temperature 25 DEG C ~ 28 DEG C, illumination every day 9 ~ 10 hours, intensity of illumination is 32.4 ~ 34.2 μm of ol.m
-2.s
-1;
Step 2, seedling packing: when growth of seedling is to about 2cm, is dispensed in blake bottle and cultivates, cultivate 4 ~ 6 strain seedling in each blake bottle;
Step 3, seedling replanting: when the seedling root length in blake bottle is 0.5 ~ 1cm, choosing 3 strain seedling is one clump, every strain seedling height of seedling 4 ~ 5cm, at least containing 5 blades, 5 corings, the medium of seedling root is first cleaned with clear water, use the potassium permanganate dilution spray disinfectant of 0.1% again, then with the line-spacing field planting of 20cm × 20cm in the matrix in booth, light irradiation apparatus, ventilation unit and humidity conditioner are set in booth;
Step 4, spraying are watered: water in the mode of spraying to the seedling just transplanted to booth, water to grabbing matrix with hand and have wet sense but do not drip and be advisable, transplant in one week, about 90% is remained on by the humidity that humidity conditioner controls in booth, after one week, the humidity controlled in booth remains on 70 ~ 80%, and adopts ventilation unit to ventilate;
Step 5, temperature treatment: the day temperature in booth controls at 25 ~ 30 DEG C, night temperatures controls at 18 ~ 20 DEG C, during summer high temperature, adopt the form cooling of spraying, and ventilation and heat, during winter low temperature, the coated sealing of booth double-plastic, prevents plant freeze injury;
Step 6, management of topdressing: seedling replanting, after two weeks, when seedling grows new root, makes foliage-spray by alga fertilizer, when treating that seedling is looked slightly a little bigger, carry out foliage spray with the general fertilizer of doctor Lu, the compound organic fertilizer material of root nutrient >=45% sprays, the amount of spraying of topdressing is few, within every two weeks, carries out once;
Step 7, illumination management: booth adopts the shade net of 70% to shelter from heat or light, and adopts light irradiation apparatus to carry out illumination management to plant, the intensity of illumination controlled in booth is 80 ~ 120 μm of ol.m
-2.s
-1;
Step 8, disease control: monthly spray with bordeaux mixture and carry out foliar control;
Step 9, pest control: booth outer cladding one deck fly net, carry out insect pest removal by the mode be manually combined with medicine to booth, prevents plant to wither.
Matrix in described step 3 becomes bits by bark sawdust, and pass through boiling water 1 hour, naturally immersion 2 ~ 3 days are cooled, dry again and form, the filling thickness of booth mesostroma is greater than 8cm, the water pH value of watering of spraying in described step 4 controls between 4.5 ~ 5.5, and described step 8 Chinese traditional medicine control can adopt equivalent formula bordeaux mixture 800 ~ 1000 times of liquid.
The dendrobium candidum that the dendrobium candidum that the embodiment of the present invention is cultivated is cultivated compared with prior art, growth rate significantly improves, and plant is comparatively large, and survival rate is high, and damage by disease and insect is less.When the dendrobium candidum that the embodiment of the present invention is cultivated enters picking time, the dendrobium candidum that the embodiment of the present invention winning the transplanting of same time section is cultivated and the dendrobium candidum that prior art is cultivated, select the more consistent dendrobium candidum of growing way and carry out measurement of the polysaccharide content, polyoses content adopts the method for phenol-sulfuric acid colorimetric to measure.
Measurement result finds through contrast, the polyoses content mean value of the dendrobium candidum that the mean value of polyoses content is cultivated apparently higher than prior art in the dendrobium candidum that the embodiment of the present invention is cultivated, show that the present invention can improve the polyoses content in dendrobium candidum, improve the medical value of dendrobium candidum.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement or improvement etc., all should be included within protection scope of the present invention.
Claims (4)
1. improve a cultivation method for polysaccharide content of dendrobium candidum, it is characterized in that: comprise the steps:
Step one, group training seedling: choose wild dendrobium candidum seedling, carry out explant cultivation, after turning out bud or leaf bud, bud or leaf bud are put into medium and carries out sterile propagation cultivation, cultivation temperature 25 DEG C ~ 28 DEG C, illumination every day 9 ~ 10 hours, intensity of illumination is 32.4 ~ 34.2 μm of ol.m
-2.s
-1;
Step 2, seedling packing: when growth of seedling is to about 2cm, is dispensed in blake bottle and cultivates, cultivate 4 ~ 6 strain seedling in each blake bottle;
Step 3, seedling replanting: when the seedling root length in blake bottle is 0.5 ~ 1cm, choosing 3 strain seedling is one clump, every strain seedling height of seedling 4 ~ 5cm, at least containing 5 blades, 5 corings, the medium of seedling root is first cleaned with clear water, use the potassium permanganate dilution spray disinfectant of 0.1% again, then with the line-spacing field planting of 20cm × 20cm in the matrix in booth, light irradiation apparatus, ventilation unit and humidity conditioner are set in booth;
Step 4, spraying are watered: water in the mode of spraying to the seedling just transplanted to booth, water to grabbing matrix with hand and have wet sense but do not drip and be advisable, transplant in one week, about 90% is remained on by the humidity that humidity conditioner controls in booth, after one week, the humidity controlled in booth remains on 70 ~ 80%, and adopts ventilation unit to ventilate;
Step 5, temperature treatment: the day temperature in booth controls at 25 ~ 30 DEG C, night temperatures controls at 18 ~ 20 DEG C, during summer high temperature, adopt the form cooling of spraying, and ventilation and heat, during winter low temperature, the coated sealing of booth double-plastic, prevents plant freeze injury;
Step 6, management of topdressing: seedling replanting, after two weeks, when seedling grows new root, makes foliage-spray by alga fertilizer, when treating that seedling is looked slightly a little bigger, carry out foliage spray with the general fertilizer of doctor Lu, the compound organic fertilizer material of root nutrient >=45% sprays, the amount of spraying of topdressing is few, within every two weeks, carries out once;
Step 7, illumination management: booth adopts the shade net of 70% to shelter from heat or light, and adopts light irradiation apparatus to carry out illumination management to plant, the intensity of illumination controlled in booth is 80 ~ 120 μm of ol.m
-2.s
-1;
Step 8, disease control: monthly spray with bordeaux mixture and carry out foliar control;
Step 9, pest control: booth outer cladding one deck fly net, carry out insect pest removal by the mode be manually combined with medicine to booth, prevents plant to wither.
2. a kind of cultivation method improving polysaccharide content of dendrobium candidum as claimed in claim 1, it is characterized in that: the matrix in described step 3 becomes bits by bark sawdust, and pass through boiling water 1 hour, naturally cool immersion 2 ~ 3 days, dry and form, the filling thickness of booth mesostroma is greater than 8cm.
3. a kind of cultivation method improving polysaccharide content of dendrobium candidum as claimed in claim 1, is characterized in that: the water pH value of watering of spraying in described step 4 controls between 4.5 ~ 5.5.
4. a kind of cultivation method improving polysaccharide content of dendrobium candidum as claimed in claim 1, is characterized in that: described step 8 Chinese traditional medicine control can adopt equivalent formula bordeaux mixture 800 ~ 1000 times of liquid.
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| CN105941106A (en) * | 2016-04-27 | 2016-09-21 | 庐江县天地禾生物技术有限公司 | Maintenance method for increasing content of dendrobium officinale polysaccharides |
| CN108739105A (en) * | 2018-05-18 | 2018-11-06 | 广西中医药大学 | A method of improving polysaccharide content of dendrobium candidum |
| CN114306590A (en) * | 2021-12-31 | 2022-04-12 | 皖西学院 | Veterinary vaccine diluent with immunity enhancement effect and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105941106A (en) * | 2016-04-27 | 2016-09-21 | 庐江县天地禾生物技术有限公司 | Maintenance method for increasing content of dendrobium officinale polysaccharides |
| CN108739105A (en) * | 2018-05-18 | 2018-11-06 | 广西中医药大学 | A method of improving polysaccharide content of dendrobium candidum |
| CN114306590A (en) * | 2021-12-31 | 2022-04-12 | 皖西学院 | Veterinary vaccine diluent with immunity enhancement effect and preparation method and application thereof |
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| CN105191769B (en) | 2017-10-31 |
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Inventor after: He Yunfang Inventor after: Teng Shiyuan Inventor after: Zheng Bingsong Inventor after: Ren Wei Inventor after: Wang Yang Inventor after: Zhong Shuanglin Inventor before: Teng Shiyuan Inventor before: He Yunfang Inventor before: Zheng Bingsong Inventor before: Ren Wei Inventor before: Wang Yang Inventor before: Zhong Shuanglin |