CN109122249A - A kind of method that LED intermittent illumination cultivates cucumber seedling - Google Patents
A kind of method that LED intermittent illumination cultivates cucumber seedling Download PDFInfo
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- CN109122249A CN109122249A CN201810661350.0A CN201810661350A CN109122249A CN 109122249 A CN109122249 A CN 109122249A CN 201810661350 A CN201810661350 A CN 201810661350A CN 109122249 A CN109122249 A CN 109122249A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention discloses a kind of methods that LED intermittent illumination cultivates cucumber seedling, include the following steps: S1: carrying out nursery after Seed Germination in Cucumber, seedling is cultivated to cotyledon and flattens completely；S2: the seedling through step S1 being placed under the LED light that the illumination period is 6:00~24:00, is carried out the intermittent illumination that the light cycle period is 15s, illumination duty ratio is 47%~67% and is handled 6~8 days；S3: the seedling after step S2 intermittent illumination being placed under the LED light that the illumination period is 6:00~18:00, and continuous illumination, which is handled, obtains the cucumber seedling of a leaf wholeheartedly for 6~8 days.The present invention is using LED as light source, and under the LED intermittent illumination processing that duty ratio is 53%, the seedling vigorous index of cucumber, Osmotic Adjustment Substances, activities of antioxidant enzymes, actual photosynthesis efficiency are integrally better than other processing.Therefore, it is handled 6~8 days using red and blue LED intermittent illumination, then carries out normal LED light filling and handle 6~8 days, or even more preferably nursery effect, and integrally saving electric energy 10% identical as continuous illumination always can be obtained, be the Optimizing Mode of cucumber seedling-raising light filling.
Description
Technical field
The present invention relates to a kind of modern agriculture seedling-raising techniques, and in particular to a kind of LED intermittent illumination cultivates cucumber seedling
Cultural method.
Background technique
Relative to even several minutes of dark reaction time of several seconds, light reaction can be completed within nanosecond or millisecond,
When using intermittent illumination, photosynthetic reaction center is being spaced by absorbing and storing less than the intermittent illumination luminous energy in 100 μ s
Period utilizes, to improve optical energy utilization efficiency.
Illumination is not only the important energy source of plant, and is morphogenesis in plant life cycle, cell growth hair
It educates and the signal of interest of gene expression.Plant experiences three kinds of light quality in luminous environment, light intensity and photoperiod spies by light receptor
Property variation, plant photosynthesis activity and photomorphogenesis signal are adjusted, to influence cell division and elongation, bud differentiation, gas
Hole such as switchs, blooms at the plant growths activity.Luminous environment regulation is the important regulating and controlling means and key link of facilities horticulture production.
Cucumber (Cucumis sativus L.) is the vegetables that China is generally cultivated, and edible value is high, and yield is big, warp
Added value of helping is high, is of great significance to China's agricultural development.
In order to realize energy-efficient light filling, hot research direction is had become using intermittent illumination regulating plant growth and is set
Apply the feasible direction of production.But it is reported at present about the research for carrying out vegetables nursery using intermittent illumination more rare.
Summary of the invention
The present invention in room conditions, by red and blue LED lamp and matrix train in the way of, cucumber seedling is carried out different
(6~8 days) intermittent illumination duty ratio is handled in short term, is filtered out the light filling mode for being conducive to cucumber seedling-raising, is reached energy-efficient
Target, for people, energy-efficient cultivation cucumber seedling provides a kind of production technology in room conditions.
The technical solution adopted in the present invention: a kind of method that LED intermittent illumination cultivates cucumber seedling, including walk as follows
It is rapid:
S1: carrying out nursery after Seed Germination in Cucumber, seedling is cultivated to cotyledon and flattens completely；
S2: the seedling through step S1 is placed under the red and blue LED lamp that the illumination period is 6:00~24:00, carries out illumination
The intermittent illumination that cycle period is 15s, illumination duty ratio is 47%~67% is handled 6~8 days；
S3: the seedling after step S2 intermittent illumination is placed in the red and blue LED lamp that the illumination period is 6:00~18:00
Under, continuous illumination, which is handled, obtains the cucumber seedling of a leaf wholeheartedly for 6~8 days.
In step S2 and S3, the intensity of illumination of LED light is 150 μm of olm-2·s-1。
In step S2 and S3, red blue light matter proportion is 1:1.
In step S2 and S3, wherein red light wavelength is 655~660nm, and blue light wavelength is 455~460nm.
In step S1, uses turf and coco bran to cultivate cucumber seedling with 1:1 mixed-matrix, poured using nutrient solution.
In step S1, the nutrient solution a great number of elements formula using 1/2 suddenly Glan formula, microelement be general formulation,
It include B:0.5mgL-1, Mn:0.5mgL-1, Zn:0.05mgL-1, Cu:0.02mgL-1, Mo:0.01mgL-1。
In step S2, it is 150 μm of olm that seedling, which is placed in intensity of illumination,-2·s-1, periodicity of illumination 15s, illumination duty
Than be 6:00~24:00 for 53%, illumination period red and blue LED intermittent illumination under cultivate 7 days, obtain best nursery effect.
In step S3, after intermittent illumination is cultivated, it is 150 μm of olm that seedling, which is placed in intensity of illumination,-2·s-1, illumination
It is cultivated 7 days under the red and blue LED continuous illumination that period is 6:00~18:00.
Compared with prior art, the beneficial effects of the present invention are: a kind of (1) LED intermittent illumination cultivates the side of cucumber seedling
Method, using under indoor conditions, by red and blue LED Control Console and matrix train in the way of, best intermittent illumination is carried out to cucumber seedling
Processing, can produce the high cucumber seedling of quality, realize energy-efficient cultivation cucumber seedling.(2) LED interval is utilized indoors
It is an efficient and energy-efficient new technology that cucumber is cultivated in illumination, and advantage is prominent.
Detailed description of the invention
Fig. 1 is influence of the different short-term intermittent illuminations of LED duty ratio to cucumber seedling seedling vigorous index.
Wherein: duty ratio CK:100%, ST1:67%, ST2:60%, ST3:53%, ST4:47% of test process.Figure
Different lowercase letter indication differences are significant (α=0.05) on column, and Fig. 2-Fig. 6 is similarly.
Fig. 2 is influence of the different short-term intermittent illuminations of LED duty ratio to cucumber seedling growth index.
Fig. 3 is influence of the different short-term intermittent illuminations of LED duty ratio to cucumber seedling seedling vigorous index.
Fig. 4 is influence of the different short-term intermittent illuminations of LED duty ratio to cucumber seedling chlorophyll fluorescence.
Fig. 5 is influence of the different short-term intermittent illuminations of LED duty ratio to cucumber seedling osmotic adjustment.
Fig. 6 is influence of the different short-term intermittent illuminations of LED duty ratio to cucumber seedling oxidation resistance.
Specific embodiment
Technical solution of the present invention is further illustrated combined with specific embodiments below.
Embodiment
1. material
It tests and is carried out on January 16,21 days to 2018 December in 2017 in College of Horticulture of Agricultural University Of South China.It is yellow for examination
Melon kind is " Guangdong show three ", and using the LED of red blue proportion 1:1 as test light source, red light wavelength is 655~660nm；It is blue
A length of 455~the 460nm of light wave.Test nursery is carried out using 50 hole hole trays.Nutrient solution a great number of elements formula is using 1/2 Glan suddenly
It is formulated (pH value ≈ 6.0), microelement is general formulation, that is, includes B:0.5mgL-1, Mn:0.5mgL-1, Zn:
0.05mg·L-1, Cu:0.02mgL-1, Mo:0.01mgL-1.It the steps include: step S1: after making Seed Germination in Cucumber, into
The seedling flattened completely to cotyledon is cultivated in row nursery；Step S2: it is 150 μm of olm that seedling, which is placed in intensity of illumination,-2·s-1、
The red and blue LED intermittent illumination that periodicity of illumination is 15s, illumination duty ratio is 67%~47%, the illumination period is 6:00~24:00
It is lower to cultivate 7 days；Step S3: after intermittent illumination is cultivated 7 days, it is 150 μm of olm that seedling, which is placed in intensity of illumination,-2·s-1, light
The cucumber seedling of a leaf wholeheartedly is obtained within 7 days according to cultivating under the red and blue LED continuous illumination that the period is 6:00~18:00.Of the invention
All cultivation techniques are not influenced in semi-enclosed indoor progress by the illumination condition under in any natural conditions.
2. test method and result
Influence of the 2.1 short-term intermittent illuminations of difference LED duty ratio to cucumber seedling growth and physiological property
It tests and is carried out on January 16,21 days to 2018 December in 2017 in College of Horticulture of Agricultural University Of South China.Cucumber kind
After son seed soaking 7 hours, be placed in 28 DEG C of growth cabinet and distinguish vernalization 24 hours, be seeded into after showing money or valuables one carries unintentionally equipped with turf and
In 50 hole hole trays of coco bran (1:1), and it is placed in day-light greenhouse.Before cotyledon is fully deployed, clear water is poured, to son
Leaf is fully deployed rear nutrient solution.When January 2, cotyledon was fully deployed, sampling statistics seedling raw biometric data, and by cave
Disk is placed in the shading layer frame for be equipped with LED and is tested.
Test 4 processing (ST1, ST2, ST3, ST4) of setting and 1 control (CK), intensity of illumination are unified for 150 μ
mol·m-2·s-1, each intermittent illumination process cycle is 15s.Specific test process is as shown in table 1.Mutually tire out on the same day to compare
The nursery effect of continuous illumination and short-term interruption illumination under the product quantity of illumination, and test energy-saving application scheme, are arranged the every sunshine of CK
Light time section is 6:00~18:00, and it is 6:00~24:00 that each short-term intermittent illumination, which handles the daily irradiation period, make CK, ST1,
The cumulative daylight of ST2, ST3 and ST4 are respectively 12h, 12h, 10.8h, 9.6h and 8.4h according to the time.Short-term intermittent illumination processing
After 7 days, CK, ST1, ST2, ST3 and ST4 illumination period are converted into 6:00~18:00 (cumulative daylight shines time 12h).Often
A processing is repeated 3 times, 50 plants of seedling of each repetition, random alignment between repetition, and black-out cloth is arranged between processing, is independent of each other.
January 16, harvesting, measured growth, physiological character, chlorophyll fluorescence and the oxidation resistance index of cucumber seedling.
The opening and closing for the DJ-D14 time switch control intermittent light source that test is produced using the precious scientific & technical corporation of timing,
And the actual power consumption amount of the D board power measurement socket record test produced using Shang De group.
The 1 short-term intermittent illumination test process of difference LED duty ratio of table
Data analysis carries out variance analysis using 14.0 software of SPSS, and LSD multiple analysis comparison method is taken to examine difference
Property (P < 0.05), using 2016 software development chart of Excel.And data record is entered into table 2~6 and Fig. 1~6.
Short-term influence of (7 days) intermittent illumination to cucumber seedling growth of 2 difference LED duty ratio of table
Note: different lowercase letter indication differences reach 5% level of signifiance, similarly hereinafter.
Short-term influence of (7 days) intermittent illumination to cucumber seedling growth of 3 difference LED duty ratio of table
The influence that the 4 short-term intermittent illumination of difference LED duty ratio of table grows cucumber seedling
The influence that the 5 short-term intermittent illumination of difference LED duty ratio of table grows cucumber seedling
Influence of the 6 short-term intermittent illumination of difference LED duty ratio of table to cucumber seedling Contents of Photosynthetic Pigments
3. result and analysis
Influence of the 3.1 short-term intermittent illuminations of difference LED duty ratio to cucumber seedling growth and physiological property
By table 2, table 3 and Fig. 1 it is found that from the point of view of comprehensive growth indexes, after short-term intermittent illumination is handled 7 days, compared with low duty ratio
Intermittent illumination processing compared with the control, significantly reduce the nursery effect of cucumber seedling.It can by table 4, table 5, Fig. 2 and Fig. 3
Know, after short-term intermittent illumination is changed into continuous illumination processing 7 days, different LED duty ratio intermittent illuminations processing cucumber seedlings are educated
Seedling effect with compare no significant difference.
Compared with CK, the chlorophyll content of ST3 and ST4 processing cucumber seedling is significantly improved, and carotenoid content is then
It significantly reduces (table 6).It can be seen that LED is compared with the short-term intermittent illumination of low duty ratio to the component ratio of cucumber seedling photosynthetic pigments
In the presence of significantly affecting.
In addition to the PSII maximum Photochemical quantum yield (Fv/Fm) of ST4 substantially reduces, Y (II), the Y of other processing
(NPQ), Y (NO), qP, qL, NPQ and qN there are no significant compared with CK difference (Fig. 4).It follows that in a certain range,
The different short-term intermittent illuminations of LED duty ratio influence cucumber seedling light-use smaller.
Short-term intermittent illumination processing does not make significant difference (Fig. 5) to the soluble sugar content of cucumber seedling；Compared with CK, 4
The soluble protein content of short-term intermittent illumination processing cucumber seedling dramatically increases, and increases 90.39% with ST4, respectively handles it
Between significant difference.It can be seen that there is cucumber seedling difference osmotic adjustment in the short-term intermittent illumination of difference LED duty ratio
Different Effects.
Compared with CK, the CAT activity of the lower cucumber seedling of ST1, ST2, ST3 and ST4 processing is dramatically increased, and is increased separately
36.65%, 16.11%, 24.16% and 27.63% (Fig. 6), significant difference between each processing.Compared with CK, between 4 short-term
Lighting process of having a rest significantly increases the POD activity of cucumber seedling.Short-term intermittent illumination is to the SOD activity of cucumber seedling without aobvious
Writing influences.It can be seen that short-term intermittent illumination processing is improved effect to the oxidation resistance of cucumber seedling.
In conclusion the ST3 that duty ratio is 53% handles seedling vigorous index, the chlorophyll content, osmotic adjustment of cucumber seedling
Content of material, activities of antioxidant enzymes, actual photosynthesis efficiency are integrally better than other processing.
4. conclusion
Although there is certain influence to the nursery effect of cucumber seedling in short-term (7 days) intermittent illumination, by being converted to
After continuous illumination, handle cucumber seedling nursery effect with compare no significant difference.In conclusion in a short time (6~8 days)
It uses duty ratio for 53%, periodicity of illumination 15s, the processing of daily irradiation 16 hours LED intermittent illuminations, can obtain and routine
Continuous illumination is identical or even more preferably nursery effect, and integrally saves electric energy 10%, is the optimization mould of cucumber seedling-raising light filling
Formula.
For those skilled in the art, it can make other each according to the above description of the technical scheme and ideas
Kind is corresponding to be changed and deforms, and all these change and deform the protection that all should belong to the claims in the present invention
Within the scope of.
Claims (8)
1. a kind of method that LED intermittent illumination cultivates cucumber seedling, characterized by the following steps:
S1: carrying out nursery after Seed Germination in Cucumber, seedling is cultivated to cotyledon and flattens completely；
S2: the seedling through step S1 is placed under the red and blue LED lamp that the illumination period is 6:00~24:00, carries out light cycle
The intermittent illumination that period is 15s, illumination duty ratio is 47%~67% is handled 6~8 days；
S3: the seedling after step S2 intermittent illumination is placed under the red and blue LED lamp that the illumination period is 6:00~18:00, even
Continuous lighting process obtains the cucumber seedling of a leaf wholeheartedly for 6~8 days.
2. the method that a kind of LED intermittent illumination according to claim 1 cultivates cucumber seedling, it is characterised in that: step S2
In S3, the intensity of illumination of LED light is 150 μm of olm-2·s-1。
3. the method that a kind of LED intermittent illumination according to claim 1 cultivates cucumber seedling, it is characterised in that: step S2
In S3, red blue light matter proportion is 1:1.
4. the method that a kind of LED intermittent illumination according to claim 1 cultivates cucumber seedling, it is characterised in that: step S2
In S3, wherein red light wavelength is 655~660nm, and blue light wavelength is 455~460nm.
5. the method that a kind of LED intermittent illumination according to claim 1 cultivates cucumber seedling, it is characterised in that: step S1
In, it uses turf and coco bran to cultivate cucumber seedling with 1:1 mixed-matrix, is poured using nutrient solution.
6. the method that a kind of LED intermittent illumination according to claim 4 cultivates cucumber seedling, it is characterised in that: step S1
In, the nutrient solution a great number of elements formula using 1/2 suddenly Glan formula, microelement be general formulation, that is, include B:
0.5mg·L-1, Mn:0.5mgL-1, Zn:0.05mgL-1, Cu:0.02mgL-1, Mo:0.01mgL-1。
7. the method that a kind of LED intermittent illumination according to claim 1 cultivates cucumber seedling, it is characterised in that: step S2
In, it is 150 μm of olm that seedling, which is placed in intensity of illumination,-2·s-1, periodicity of illumination 15s, illumination duty ratio be 53%, illumination when
It is cultivated 7 days under the red and blue LED intermittent illumination that section is 6:00~24:00, obtains best nursery effect.
8. the method that a kind of LED intermittent illumination according to claim 1 cultivates cucumber seedling, it is characterised in that: step S3
In, after intermittent illumination is cultivated, it is 150 μm of olm that seedling, which is placed in intensity of illumination,-2·s-1, the illumination period be 6:00~18:
It is cultivated 7 days under 00 red and blue LED continuous illumination.
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| CN110521566A (en) * | 2019-09-25 | 2019-12-03 | 福建省中科生物股份有限公司 | A kind of luminous environment regulation method of regulation plant metabolism substance |
| CN111448903A (en) * | 2020-04-30 | 2020-07-28 | 上海市农业科学院 | Light-controlled pepper seedling growing method and illumination equipment |
| CN111448904A (en) * | 2020-04-30 | 2020-07-28 | 上海市农业科学院 | Light-control cucumber seedling raising method and illumination equipment |
| CN113728907A (en) * | 2021-09-18 | 2021-12-03 | 天津市金舟科技发展有限公司 | Method for regulating crop seedling by artificial light source |
| CN114586561A (en) * | 2022-03-18 | 2022-06-07 | 华南农业大学 | Method for cultivating tomato seedlings by LED pulse illumination |
| CN115136857A (en) * | 2022-06-28 | 2022-10-04 | 华南农业大学 | Method for cultivating strong cucumber seedlings by adopting segmented light supplement |
| CN115413543A (en) * | 2022-10-17 | 2022-12-02 | 华南农业大学 | Method for improving yield of pakchoi seedling vegetables by LED intermittent illumination |
| CN115918529A (en) * | 2022-09-28 | 2023-04-07 | 山东惠美农牧发展有限公司 | Lily test tube bulb proliferation method |
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| CN110073837A (en) * | 2019-04-16 | 2019-08-02 | 北京农业智能装备技术研究中心 | The method and light source illuminating apparatus for promoting vegetables are irradiated using discontinuous light |
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| CN110495318A (en) * | 2019-09-25 | 2019-11-26 | 福建省中科生物股份有限公司 | A kind of illumination method of regulation plants ' reproduction development |
| CN110521566A (en) * | 2019-09-25 | 2019-12-03 | 福建省中科生物股份有限公司 | A kind of luminous environment regulation method of regulation plant metabolism substance |
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| CN111448903A (en) * | 2020-04-30 | 2020-07-28 | 上海市农业科学院 | Light-controlled pepper seedling growing method and illumination equipment |
| CN111448904A (en) * | 2020-04-30 | 2020-07-28 | 上海市农业科学院 | Light-control cucumber seedling raising method and illumination equipment |
| CN113728907A (en) * | 2021-09-18 | 2021-12-03 | 天津市金舟科技发展有限公司 | Method for regulating crop seedling by artificial light source |
| CN114586561A (en) * | 2022-03-18 | 2022-06-07 | 华南农业大学 | Method for cultivating tomato seedlings by LED pulse illumination |
| CN115136857A (en) * | 2022-06-28 | 2022-10-04 | 华南农业大学 | Method for cultivating strong cucumber seedlings by adopting segmented light supplement |
| CN115918529A (en) * | 2022-09-28 | 2023-04-07 | 山东惠美农牧发展有限公司 | Lily test tube bulb proliferation method |
| CN115918529B (en) * | 2022-09-28 | 2024-05-28 | 山东惠美农牧发展有限公司 | Lily test tube bulb proliferation method |
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