CN108713486A - Strawberry Water culture method for culturing seedlings under a kind of artificial light environment - Google Patents
Strawberry Water culture method for culturing seedlings under a kind of artificial light environment Download PDFInfo
- Publication number
- CN108713486A CN108713486A CN201810539987.2A CN201810539987A CN108713486A CN 108713486 A CN108713486 A CN 108713486A CN 201810539987 A CN201810539987 A CN 201810539987A CN 108713486 A CN108713486 A CN 108713486A
- Authority
- CN
- China
- Prior art keywords
- culture
- seedling
- strawberry
- cuttage
- illumination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to breeding plant technical field, strawberry Water culture method for culturing seedlings under a kind of artificial light environment is specifically disclosed.Strawberry cuttage seeding is subjected to culture of rootage with nutrient solution using culture of rootage under artificial light environment, intensity of illumination is 25 μm of ol/m2/ s~145 μm ol/m2/s；After culture grows the adventitious root of 5~7 1.5~2.5cm to cuttage seeding, seedling culture is carried out under artificial light environment with nutrient solution using seedling culture, intensity of illumination is 90 μm of ol/m2/ s~270 μm ol/m2/s.The present invention provides suitable intensity of illumination for strawberry cuttage seeding using LED light source, promotes the accumulation of cuttage seeding photosynthate, accelerates cuttage seedling rooting, and without additionally using plant growth regulator, cuttage survival rate is up to 100%.
Description
Technical field
The invention belongs to breeding plant technical fields, specifically, being related to strawberry Water culture under a kind of artificial light environment
Method for culturing seedlings.
Background technology
Strawberry cuttage and seedling culture is will not to take root on strawberry mother's seedling stolon or have the sub- seedling of the stolon of a small amount of adventitious root to adopt down
Afterwards, detached insertion is cultivated to the technology of seedling, is had and is shortened growing-seedling period, reduce labor intensity, improve seedling regularity, be conducive to
The advantages that nurturing staff is the important method of strawberry seedling breeding.Since the sub- seedling under being adopted from stolon is not taken root or is only had
A small amount of adventitious root and root carry shearing wound, therefore promote cuttage seedling rooting and prevent soil-borne disease from infecting to be strawberry cuttage and seedling culture
Key technology, directly affect cuttage survival rate and strawberry seedling quality.
The Chinese patent application of Publication No. CN103688835A discloses strawberry potted tray cuttage and seedling culture method, Publication No.
The Chinese patent application of CN106258368 discloses a kind of new method of the efficient sprouting and rooting of strawberry stolon, and cuttage seeding is dark
It takes root after being handled 6~14 days under the conditions of black, planting percent is still relatively low, and two methods need frequent applicationization during seedling culture
It learns to farm medicine controlling disease, strawberry seedling agriculture is residual higher, does not meet strawberry green production theory.Publication No. CN106561330A's
Although Chinese patent application improves disease resistance of the strawberry cuttage seeding during crop field is cultivated, but cuttage seeding sterilization, rooting treatment
Process is extremely complex, substantially increases labor intensity.
Water planting is conducive to the generation for preventing soil-borne disease as a kind of cultivation technique without soil of clean and effective.Publication No.
The Chinese patent application of CN103641604A discloses a kind of water-cultured seedling of strawberry nutrient solution, but it does not illustrate how to utilize and be somebody's turn to do
Nutrient solution carries out strawberry nursery, and nutrient composition is complicated, and process for preparation is cumbersome, and survival rate of seedling is not also high.Publication No.
The Chinese patent application of CN106069660A disclose a kind of strawberry without the matrix hydroponic method, Publication No. CN105918093A
Chinese patent application disclose the pipeline cultural method of strawberry, but be after surviving strawberry seedling disclosed in two methods
Phase cultivation technique is not related to strawberry seedling-raising technique, is not related to the cuttage raise seedling technique of strawberry especially.
Invention content
In order to solve the problems in the existing technology, the object of the present invention is to provide strawberry water under a kind of artificial light environment
Cuttage and seedling culture method is trained, strawberry cuttage and seedling culture survival rate, reduction labor intensity are improved to reach, in the case of not applying pesticides
Cultivate the purpose of the high-quality strong sprout of strawberry.
In order to realize the object of the invention, technical scheme is as follows：
The present invention provides strawberry Water culture method for culturing seedlings under a kind of artificial light environment, and strawberry cuttage seeding is utilized and is taken root
Culture carries out culture of rootage with nutrient solution under artificial light environment, and intensity of illumination is 25 μm of ol/m2/ s~145 μm ol/m2/s；Training
After supporting to cuttage seeding the adventitious root for growing 5~7 1.5~2.5cm, using seedling culture nutrient solution under artificial light environment into
Row seedling culture, intensity of illumination are 90 μm of ol/m2/ s~270 μm ol/m2/s。
Wherein, the culture of rootage spends ionized water based on basal nutrient formula of liquid with nutrient solution and prepares, and uses deionized water
EC values are adjusted to 0.2~0.4mS/cm, pH value is adjusted to 6.0~6.5 with hydrochloric acid or sodium hydroxide solution.The seedling culture is used
Nutrient solution is based on basal nutrient formula of liquid and is prepared using purified water, and EC values are adjusted to 0.6~0.8mS/cm with deionized water, use salt
PH value is adjusted to 6.0~6.5 by acid or sodium hydroxide solution.
Wherein, the basal nutrient formula of liquid is potassium nitrate (KNO3)：303mg/L, calcium nitrate (Ca (NO3)2·4H2O)：
236mg/L, ammonium dihydrogen phosphate (NH4H2PO4)：57mg/L, magnesium sulfate (MgSO4)：123mg/L, chelated iron (Na2Fe7-EDTA)：
28.6mg/L, boric acid (H3BO3)：1.13mg/L, manganese sulfate (MnSO4·H2O)：0.61mg/L, zinc sulfate (ZnSO4·7H2O)：
0.09mg/L, copper sulphate (CuSO4·5H2O)：0.04mg/L, ammonium molybdate ((NH4)6Mo7O24·4H2O)：0.01mg/L.
Further, the intensity of illumination of the culture of rootage can be 25 μm of ol/m2/ s~85 μm ol/m2/ s or 85 μm of ol/
m2/ s~145 μm ol/m2/s。
It should be noted that artificial light environment refers to growing required luminous environment to strawberry at least to be passed through by a part
LED light provides, and does not imply that and does not use sunlight completely, and the luminous environment that sunlight and LED light are combined to creation is equally applicable
In the present invention.The present invention is not particularly limited the spectral distribution property of LED light, but its should be able to provide wave-length coverage be 300~
The physiology Net long wave radiation of 800nm can meet the light of the required each wavelength of strawberry cuttage seeding normal growth and development.Light application time
It is not particularly limited, but according to known, the long-day is unfavorable for the bud differentiation of strawberry, and too short sunshine is unfavorable for photosynthate
Accumulation, influences the speed of growth, therefore periodicity of illumination is preferably in 12~14h/d.
Preferably, during culture of rootage and seedling culture, periodicity of illumination is set as 12h/d.
Further, the artificial light source of the artificial light environment is LED light.
The artificial light environment is based on white light, preferably pure white light.
It should be noted that adulterating feux rouges in right amount on the basis of white light also belongs to a kind of feasible program.The present invention's
In one specific implementation mode, with pure white LED, Bai Hong LED5:1 and Bai Hong LED5:3 are used as artificial light source, and pure white LED refers to this
LED light is made of White LED lamp bead completely, Bai Hong LED5:1 refers to the LED light presses 5 by White LED lamp bead and red LED lamp bead:1
Quantity be made, Bai Hong LED5:3 refer to the LED light presses 5 by White LED lamp bead and red LED lamp bead:3 quantity is made, three kinds
All based on white luminous environment, the survival rate of the strawberry cuttage seeding grown under three kinds of LED light environment is equal for the illumination that LED light provides
Reach 100%, high-quality strong sprout requirement is reached in harvest.Plant height, stem thickness, the number of blade, the overground part dry weight of strawberry cuttage seeding
And the indexs such as maximum light energy use efficiency of PS II of blade there are no significant difference, show three kinds of LED based on white light
Luminous environment can meet the needs of strawberry growth of cuttage seedlings development.
Further, the present invention is also optimized the condition of culture of culture of rootage and seedling culture, training of preferably taking root
The air themperature for the process of supporting is 18~23 DEG C, and relative humidity is 70~90%, and gas concentration lwevel is 750~850ppm；Seedling
Air themperature in incubation is 18~23 DEG C, and relative humidity is 60~80%, and gas concentration lwevel is 750~850ppm.
For complete actual mechanical process, the method for the invention includes the following steps：
(1) cuttage seeding is chosen：From high-quality virus-free strawberry mother seedling generate stolon on harvesting have 2~3 expansion leaves and
Cripetura basal part of stem has the sub- seedling of stolon of a small amount of 0.5~1.0cm adventitious roots as cuttage seeding；
(2) culture of rootage：By the cuttage seeding cuttage of collection in sponge block, using culture of rootage nutrient solution in LED people
Allow its Rapid Rooting under work luminous environment；
(3) seedling culture：After having lateral root generation on the adventitious root of the cuttage seeding of under growth root culture, sought using seedling culture
Nutrient solution continues to cultivate under LED artificial light environments, and cuttage seeding is grown to 6 expansion leaves, and stem thickness can harvest when being 0.8~1.0cm
Strawberry seedling.
On the basis of common knowledge of the art, above-mentioned each optimum condition, can be combined with each other, obtain specific embodiment party
Formula.
The beneficial effects of the present invention are：
The present invention provides suitable intensity of illumination for strawberry cuttage seeding using LED light source, promotes cuttage seeding photosynthate
Cuttage seedling rooting is accelerated in accumulation, and without additionally using plant growth regulator, cuttage survival rate is up to 100%.
The present invention is conducive to prevent the generation of soil-borne disease using water planting mode nursery, can be anti-without using chemical pesticide
High-quality strong sprout is cultivated in the case of controlling soil-borne disease, realizes green production.
The present invention carries out strawberry under artificial light environment easy to operate, the Ke Yiyou of water planting culture of rootage and seedling culture
Effect reduces labor intensity, improves cuttage and seedling culture efficiency.
The present invention cultivates strawberry water planting seedling under artificial light environment, has energy saving and easy-regulating effect using LED light source
Fruit is suitable for technological industrialization application and large-scale production.
Description of the drawings
Fig. 1 is the spectral distribution graph of the LED light used in embodiment 1 and embodiment 2
Fig. 2 is the block diagram that intensity of illumination influences strawberry cuttage seeding root growth in embodiment 1.
Fig. 3 is intensity of illumination in embodiment 1 to the block diagram of strawberry cuttage seeding leaf photosynthesis activity influence.
Specific implementation mode
With reference to embodiment the present invention will be further explained explanation.It will be appreciated that following embodiment provides
Merely to playing the purpose of explanation, it is not used to limit the scope of the present invention.Those skilled in the art is not
In the case of spirit of the invention and spirit, the present invention can be carry out various modifications and be replaced.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1
It selects uniform in size consistent, there is " beauty " strawberry of 3 expansion leaves and 2~3 0.5~1.0cm adventitious roots to crawl
For stem seedling as cuttage seeding, cuttage carries out culture of rootage with nutrient solution in sponge block, using culture of rootage under LED light.It is raw
Root culture nutrient solution is based on basal nutrient formula of liquid using purified water preparation, EC values are adjusted to 0.2 with deionized water~
PH value is adjusted to 6.0~6.5 by 0.4mS/cm with hydrochloric acid or sodium hydroxide solution.Basal nutrient formula of liquid is potassium nitrate (KNO3)：
303mg/L, calcium nitrate (Ca (NO3)2·4H2O)：236mg/L, ammonium dihydrogen phosphate (NH4H2PO4)：57mg/L, magnesium sulfate
(MgSO4)：123mg/L, chelated iron (Na2Fe7-EDTA)：28.6mg/L, boric acid (H3BO3)：1.13mg/L, manganese sulfate
(MnSO4·H2O)：0.61mg/L, zinc sulfate (ZnSO4·7H2O)：0.09mg/L, copper sulphate (CuSO4·5H2O)：0.04mg/
L, ammonium molybdate ((NH4)6Mo7O24·4H2O)：0.01mg/L.
The LED light of use is made of White LED lamp bead completely, the spectrum of spatial distribution pure white LED as shown in Figure 1.
The canopy intensity of illumination of strawberry cuttage seeding is respectively set to 25 μm of ol/m2/s、85μmol/m2/s、145μmol/m2/ s and 205 μ
mol/m2/ s, periodicity of illumination 12h/d carry out strawberry nursery under each intensity of illumination using 9 plants of cuttage seedings.Culture of rootage mistake
The air themperature of journey is 18~23 DEG C, and relative humidity is 70~90%, and gas concentration lwevel is 750~850ppm.Cuttage 6 days
Afterwards, cuttage seeding all survives, and has lateral root generation on adventitious root, is selected at random in the cuttage seeding grown under above-mentioned 4 kinds of intensities of illumination
6 plants are taken, adventitious root quantity and root system average length that its length is more than 1cm is counted, more work(of Hansatech companies of Britain is used in combination
Energy Plant Efficiency analyzer (M-PEA) measures the Chlorophyll Fluorescence of strawberry seedling leaf.
The effect of cuttage seeding culture of rootage under different illumination intensity is illustrated in conjunction with Fig. 2 and Fig. 3：Strawberry cuttage seeding
Root quantity is reduced afterwards as the increase of intensity of illumination first increases, in 85 μm of ol/m2It is maximum when/s intensities of illumination, but intensity of illumination
Not generating conspicuousness to root system average length influences.In 25 μm of ol/m2/s、85μmol/m2/ s and 145 μm of ol/m2/ s intensities of illumination
There was no significant difference for the maximum light energy use efficiencies of PS II of the strawberry cuttage seedling leaf of lower growth, but is apparently higher than 205 μm of ol/
m2Value under/s intensities of illumination.In 145 μm of ol/m2/ s and 205 μm of ol/m2The strawberry cuttage seedling leaf cultivated under/s intensities of illumination
The photosynthetic performance index based on absorbing luminous energy be substantially less than 25 μm of ol/m2/ s and 85 μm of ol/m2Under/s intensities of illumination,
This shows the cuttage seeding in the stage of taking root in 145 μm of ol/m2/ s and 205 μm of ol/m2Xanthophyll cycle is received under/s intensities of illumination.Therefore,
Intensity of illumination during strawberry cuttage seedling rooting should control should be in 145 μm of ol/m2/ s or less.
In 25 μm of ol/m2/ s~145 μm ol/m2Under/s intensities of illumination, it is average that 6 days cuttage seedings of culture of rootage grow 5-7 items
The adventitious root of length 2cm, and have lateral root generation on adventitious root, root growth is good, into the seedling culture of next stage.
It selects in 85 μm of ol/m2The uniform cuttage seeding of growing way that culture of rootage is completed under/s intensities of illumination continues at this
Seedling culture is carried out under White LED artificial light environment.Seedling culture spends ionized water with nutrient solution based on basal nutrient formula of liquid
It prepares, EC values is adjusted to 0.6~0.8mS/cm with deionized water, pH value is adjusted to 6.0 with hydrochloric acid or sodium hydroxide solution~
6.5.The intensity of illumination of strawberry cuttage seeding canopy is respectively set to 90 μm of ol/m2/s、180μmol/m2/s、270μmol/m2/ s and
360μmol/m2/ s, periodicity of illumination 12h/d utilize 16 plants of cuttage seedings to carry out cuttage and seedling cultures under each intensity of illumination.Seedling is trained
Air themperature during supporting is 18~23 DEG C, and relative humidity is 60~80%, and gas concentration lwevel is 750~850ppm.At
After seedling culture 12 days, 6 plants are randomly selected from the strawberry cuttage seeding grown under 4 kinds of intensities of illumination, measures its plant height, stem thickness, leaf
The indexs such as the piece number, overground part fresh weight/dry weight and underground part fresh weight/dry weight, and calculate seedling vigorous index.Seedling vigorous index (g/ plants)=
(stem thickness (cm)/plant height (cm)+underground part dry weight (g/ plants)/overground part dry weight (g/ plants)) × complete stool dry weight (g/ plants).
The influence that the intensity of illumination of 1 seedling incubation of table develops strawberry growth of cuttage seedlings
Note：Different letters represent significant difference in same column, and same letter indicates that there was no significant difference (P=0.05).
The effect of strawberry cuttage seeding seedling culture under different illumination intensity is illustrated in conjunction with table 1：In 90 μm of ol/m2/
s、180μmol/m2/ s and 270 μm of ol/m2Under/s intensities of illumination, with the increase of intensity of illumination, the plant height of cuttage seeding gradually drops
Low, the indexs such as stem thickness, underground part fresh weight, overground part dry weight, underground part dry weight and seedling vigorous index increase, but overground part fresh weight
Between there was no significant difference, therefore in 90 μm of ol/m2/ s~270 μm ol/m2Within the scope of/s intensities of illumination, with intensity of illumination
Increase, is conducive to accumulation and the nurturing staff of cuttage seeding biomass.Due to 270 μm of ol/m2/ s and 360 μm of ol/m2/ s intensities of illumination
Under strawberry cuttage seeding each index size there are no significant difference, therefore intensity of illumination need not be more than 270 μm of ol/m2/s。
In 90 μm of ol/m2/ s~270 μm ol/m2Under/s intensities of illumination, the strawberry cuttage seeding after seedling culture 12 days is grown to 6
Leaf is unfolded in piece, and stem thickness is 0.8~1.0cm, root system and cauline leaf robust growth, reaches the standard of the high-quality strong sprout of strawberry, can carry out
Strawberry seedling harvests.
Embodiment 2
It selects uniform in size consistent, there is " beauty " strawberry of 3 expansion leaves and 2~3 0.5~1.0cm adventitious roots to crawl
Stem seedling is as cuttage seeding, and cuttage is in sponge block, using culture of rootage nutrient solution in 3 kinds of LED light (pure white LED, Bai Hong
LED5:1 and Bai Hong LED5:3) culture of rootage is carried out under, there are 100 plants of cuttage seedings under each LED light.Pure white LED refers to the LED light
It is made completely of White LED lamp bead, Bai Hong LED5:1 refers to the LED light presses 5 by White LED lamp bead and red LED lamp bead:1 number
Amount is made, Bai Hong LED5:3 refer to the LED light presses 5 by White LED lamp bead and red LED lamp bead:3 quantity is made, three kinds of LED light
Although the illumination of offer is all based on white luminous environment, its spatial distribution difference (Fig. 1).Culture of rootage is based on base with nutrient solution
Plinth nutrient solution prescription is prepared using purified water, EC values is adjusted to 0.2~0.4mS/cm with deionized water, with hydrochloric acid or sodium hydroxide
PH value is adjusted to 6.0~6.5 by solution.Basal nutrient formula of liquid is potassium nitrate (KNO3)：303mg/L, calcium nitrate (Ca (NO3)2·
4H2O)：236mg/L, ammonium dihydrogen phosphate (NH4H2PO4)：57mg/L, magnesium sulfate (MgSO4)：123mg/L, chelated iron (Na2Fe7-
EDTA)：28.6mg/L, boric acid (H3BO3)：1.13mg/L, manganese sulfate (MnSO4·H2O)：0.61mg/L, zinc sulfate (ZnSO4·
7H2O)：0.09mg/L, copper sulphate (CuSO4·5H2O)：0.04mg/L, ammonium molybdate ((NH4)6Mo7O24·4H2O)：0.01mg/L.
The canopy intensity of illumination of strawberry cuttage seeding is set as 100 μm of ol/m during culture of rootage2/ s, periodicity of illumination 14h/
d.Air themperature is 18~23 DEG C, and relative humidity is 70~90%, and gas concentration lwevel is 750~850ppm.Culture of rootage 6 days
Cuttage seeding all survive, and grow the adventitious root that 5-7 average length is 2cm, and have lateral root generation on adventitious root, then will
The canopy intensity of illumination of strawberry cuttage seeding is set as 200 μm of ol/m2/ s, relative air humidity control is 60~80%, nutrient solution
It is changed to seedling culture nutrient solution.Seedling culture spends ionized water based on basal nutrient formula of liquid with nutrient solution and prepares, EC
Value is adjusted to 0.6~0.8mS/cm, and pH value is adjusted to 6.0~6.5.After seedling culture 12 days, the grass that is grown under 3 kinds of LED light environment
12 plants are respectively extracted in certain kind of berries cuttage seeding, measure the indexs such as its plant height, stem thickness, the number of blade, overground part dry weight and underground part dry weight, and count
Calculate seedling vigorous index.Seedling vigorous index (g/ plants)=(stem thickness (cm)/plant height (cm)+underground part dry weight (g/ plants)/overground part dry weight (g/
Strain)) × complete stool dry weight (g/ plants).Strawberry is measured with the multi-functional plant efficiency analysis instrument (M-PEA) of Hansatech companies of Britain
Maximum light energy use efficiency (the F of PS II of cuttage seedling leafv/Fm) and photosynthetic performance index (PI based on absorbing luminous energyabs)。
2 LED light confrontation strawberry growth of cuttage seedlings of table is developed and the active influence of leaf photosynthesis
Note：Different letters represent significant difference in same column, and same letter indicates that there was no significant difference (P=0.05).
It is illustrated in conjunction with the effect that table 2 develops the strawberry growth of cuttage seedlings under 3 kinds of LED light environment：In three kinds of LED lights
The survival rate of the strawberry cuttage seeding grown under environment reaches 100%, and high-quality strong sprout requirement is reached in harvest.Strawberry cuttage
There are no significant for the indexs such as the plant height of seedling, the maximum light energy use efficiencies of PS II of stem thickness, the number of blade, overground part dry weight and blade
Difference shows that three based on white light kinds of LED light environment can meet the needs of strawberry growth of cuttage seedlings development.But
White red LED5:The underground part dry weight and seedling vigorous index of the strawberry cuttage seeding of 3 times growths are relatively low, and blade is based on absorbing luminous energy
Photosynthetic performance index it is higher, show that excessive feux rouges is added in white light is unfavorable for nurturing staff, but be conducive to improve leaf
Utilization ratio of the piece to luminous energy.Therefore, the spatial distribution that White LED light can be suitably adjusted during strawberry cuttage and seedling culture, than
Partial white LED lamp bead such as is replaced with red LED lamp bead, may be implemented while meeting growth of cuttage seedlings development and needing energy saving
Purpose.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (10)
1. strawberry Water culture method for culturing seedlings under a kind of artificial light environment, which is characterized in that strawberry cuttage seeding is utilized training of taking root
It supports and carries out culture of rootage under artificial light environment with nutrient solution, intensity of illumination is 25 μm of ol/m2/ s~145 μm ol/m2/s；Culture
After the adventitious root for growing 5~7 1.5~2.5cm to cuttage seeding, carried out under artificial light environment with nutrient solution using seedling culture
Seedling culture, intensity of illumination are 90 μm of ol/m2/ s~270 μm ol/m2/s。
2. according to the method described in claim 1, it is characterized in that, the pH value of the culture of rootage nutrient solution be 6.0~
6.5, EC values are 0.2~0.4mS/cm.
3. method according to claim 1 or 2, which is characterized in that the pH value of the seedling culture nutrient solution be 6.0~
6.5, EC values are 0.6~0.8mS/cm.
4. according to the method described in claim 1, it is characterized in that, the intensity of illumination of culture of rootage is 85 μm of ol/m2/s。
5. according to Claims 1 to 4 any one of them method, which is characterized in that in culture of rootage and seedling culture, illumination
Period is 12~14h/d.
6. according to the method described in claim 5, it is characterized in that, the artificial light environment is based on white light.
7. according to the method described in claim 6, it is characterized in that, the artificial light source of the artificial light environment is LED light.
8. according to claim 1~7 any one of them method, which is characterized in that the air themperature of process of rooting culture is 18
~23 DEG C, relative humidity is 70~90%, and gas concentration lwevel is 750~850ppm.
9. according to claim 1~8 any one of them method, which is characterized in that the air themperature in seedling incubation is
18~23 DEG C, relative humidity is 60~80%, and gas concentration lwevel is 750~850ppm.
10. according to claim 1~9 any one of them method, which is characterized in that include the following steps：
(1) cuttage seeding is chosen：Harvesting has 2~3 expansion leaves and cripetura from the stolon that high-quality virus-free strawberry mother seedling generates
Basal part of stem has the sub- seedling of stolon of a small amount of 0.5~1.0cm adventitious roots as cuttage seeding；
(2) culture of rootage：By the cuttage seeding cuttage of collection in sponge block, using culture of rootage nutrient solution in LED artificial lights
Allow its Rapid Rooting under environment；
(3) seedling culture：After having lateral root generation on the adventitious root of the cuttage seeding of under growth root culture, seedling culture nutrient solution is utilized
Continue to cultivate under LED artificial light environments, cuttage seeding is grown to 6 expansion leaves, and stem thickness can harvest strawberry when being 0.8~1.0cm
Seedling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810539987.2A CN108713486B (en) | 2018-05-30 | 2018-05-30 | Strawberry water culture cutting seedling method in artificial light environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810539987.2A CN108713486B (en) | 2018-05-30 | 2018-05-30 | Strawberry water culture cutting seedling method in artificial light environment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108713486A true CN108713486A (en) | 2018-10-30 |
| CN108713486B CN108713486B (en) | 2021-03-09 |
Family
ID=63911588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810539987.2A Active CN108713486B (en) | 2018-05-30 | 2018-05-30 | Strawberry water culture cutting seedling method in artificial light environment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108713486B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583467A (en) * | 2019-10-16 | 2019-12-20 | 广东圣之禾生物科技有限公司 | Automatic aeroponic rapid rooting and planting system and method for cuttage plants |
| KR102652680B1 (en) * | 2023-05-03 | 2024-03-28 | (주)이코모스 | strawberry cultivation system |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012196202A (en) * | 2011-03-08 | 2012-10-18 | Meiji Univ | Method for cultivating plant and device for cultivating plant |
| CN103329788A (en) * | 2013-07-11 | 2013-10-02 | 江苏沿海地区农业科学研究所 | Method for inducing elephant grass to become seedling fast in soilless culture |
| CN103518623A (en) * | 2013-10-23 | 2014-01-22 | 镇江市丹徒区紫杉生态农业园 | Fast breeding method for strawberry seedlings |
| CN203537919U (en) * | 2013-07-29 | 2014-04-16 | 合肥瑞谷农业科技有限公司 | Strawberry tissue culture support |
| CN104137772A (en) * | 2013-05-06 | 2014-11-12 | 上海市农业科学院 | A strawberry stem tip rapid breeding method by tissue culture and virus removal |
-
2018
- 2018-05-30 CN CN201810539987.2A patent/CN108713486B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012196202A (en) * | 2011-03-08 | 2012-10-18 | Meiji Univ | Method for cultivating plant and device for cultivating plant |
| CN104137772A (en) * | 2013-05-06 | 2014-11-12 | 上海市农业科学院 | A strawberry stem tip rapid breeding method by tissue culture and virus removal |
| CN103329788A (en) * | 2013-07-11 | 2013-10-02 | 江苏沿海地区农业科学研究所 | Method for inducing elephant grass to become seedling fast in soilless culture |
| CN203537919U (en) * | 2013-07-29 | 2014-04-16 | 合肥瑞谷农业科技有限公司 | Strawberry tissue culture support |
| CN103518623A (en) * | 2013-10-23 | 2014-01-22 | 镇江市丹徒区紫杉生态农业园 | Fast breeding method for strawberry seedlings |
Non-Patent Citations (3)
| Title |
|---|
| 于丽杰等: "《植物组织培养教程》", 31 August 2015, 华中科技大学出版社 * |
| 周寿荣等: "《饲料生产手册》", 31 January 2004, 四川科学技术出版社 * |
| 王鹄生: "《花卉蔬菜无土栽培技术》", 31 March 1993, 湖南科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583467A (en) * | 2019-10-16 | 2019-12-20 | 广东圣之禾生物科技有限公司 | Automatic aeroponic rapid rooting and planting system and method for cuttage plants |
| KR102652680B1 (en) * | 2023-05-03 | 2024-03-28 | (주)이코모스 | strawberry cultivation system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108713486B (en) | 2021-03-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102860258B (en) | Clonal tissue culture breeding method for camphor tree | |
| JP2022518259A (en) | Light irradiation method that promotes plant growth, plant lamps and their applications | |
| CN103202232B (en) | Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings | |
| CN111699972A (en) | Construction method of breeding system of virus-free seed potatoes | |
| CN103858758A (en) | Strawberry seedling tissue culture rapid propagation technology | |
| CN113099971B (en) | Method for promoting industrial cannabis to bloom in advance and application thereof | |
| CN101889531A (en) | Method for controlling indoor seedling raising of upland cotton | |
| CN103975834A (en) | In-house production technology for hydroponic swamp cabbages | |
| CN103947469A (en) | Light source control technology for indoor culture of red soybean sprouting vegetables | |
| CN103461121A (en) | Ex-vitro rooting method for tissue culture seedlings of pinus massoniana | |
| CN111418463A (en) | L ED artificial light environment cultivation method suitable for rice whole growth period | |
| LU501051B1 (en) | Method for Screening Saline-alkali Tolerant Wheat Germplasm at Seedling Stage Based on Aseptic Hydroponics Condition | |
| CN101209026B (en) | Outer test-tube rooting method for aralia mandshurica tissue culture seedling | |
| CN103460971A (en) | Method for improving transplanting survival rate of trichosanthes kirilowii tissue culture seedlings | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN101926284B (en) | Monkshood-tuber tissue culture and rapid propagation method | |
| CN114946856A (en) | Application of 2-amino-3-hydroxy-3-methylbutyric acid in promoting plant growth | |
| CN108713486A (en) | Strawberry Water culture method for culturing seedlings under a kind of artificial light environment | |
| CN114794113A (en) | Use of 2-amino-3-phenylbutyric acid or derivatives thereof as plant growth regulators | |
| CN105900641A (en) | Osmanthus tree cultivation method | |
| CN108967192A (en) | A kind of Sweetpotato Viruses Elimination bottle seedling acclimatization and transplants method | |
| CN108713485A (en) | A method of improving leaf vegetables Vc contents in plant factor | |
| CN106888798A (en) | A kind of facility cultivation method of ginseng without dormancy | |
| CN101341847B (en) | Continuous cultivation method for vertical seedling of lianas vegetables | |
| CN102960247A (en) | Method for obtaining China fir tissue cultured explants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |