CN108713485A - A method of improving leaf vegetables Vc contents in plant factor - Google Patents

A method of improving leaf vegetables Vc contents in plant factor Download PDF

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Publication number
CN108713485A
CN108713485A CN201810535391.5A CN201810535391A CN108713485A CN 108713485 A CN108713485 A CN 108713485A CN 201810535391 A CN201810535391 A CN 201810535391A CN 108713485 A CN108713485 A CN 108713485A
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China
Prior art keywords
light
illumination
contents
plant factor
plant
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CN201810535391.5A
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Chinese (zh)
Inventor
张更
颜志明
矢守航
杨宝林
董慧
王祺
邰智凡
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Jiangsu Polytechnic College of Agriculture and Forestry
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Jiangsu Polytechnic College of Agriculture and Forestry
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Priority to CN201810535391.5A priority Critical patent/CN108713485A/en
Priority to PCT/CN2018/100283 priority patent/WO2019227680A1/en
Publication of CN108713485A publication Critical patent/CN108713485A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • A01G7/045Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Hydroponics (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of methods of leaf vegetables Vc contents in raising plant factor, including:Pregermination and seedling breeding;The vegetable seedling cultivated is transplanted to cultivating on the cultivating stand of plant factor, carries out top light photograph within 6~8 days before culture, resynchronisation later carries out that top light is shone and the continuous light filling in bottom, bottom light filling harvest after 7~10 days;Wherein, the direction of illumination that top light is shone is to be irradiated from romaine lettuce top to bottom;When the continuous light filling in bottom, the direction of illumination of illumination is to be irradiated from romaine lettuce bottom to top, and intensity of illumination is 40~60 μm of olm‑2·s‑1, light application time is for 24 hours/day.The present invention improves current artificial light using the illumination method of type plant factor, in such a way that the Later growth in plant carries out continuous light filling from plant bottom, improves the Vc contents in plant leaf, improves product quality.

Description

A method of improving leaf vegetables Vc contents in plant factor
Technical field
The invention belongs to vegetable growing and quality control technique fields, and in particular to leaf vegetables in a kind of raising plant factor The method of vegetables Vc contents.
Background technology
Plant factor's production system is a kind of resource-intensive approach production model, can greatly improve the utilization in unit soil Rate, output capacity and economic benefit (Liu Wenke etc., 2014, illuminating engineering journal, 25 (4):50-53).Plant factor is according to illumination The difference in source is divided into artificial light using type, sunlight using type and sunlight and artificial light and with type three categories.Artificial light Do not restricted by region, natural conditions using type plant factor, can environment in precision controlling facility, provided for plant optimum Growth conditions, plant growing way is good, yield is high, quality is excellent, with short production cycle, the production schedule is strong, develops ten in China in recent years Point rapid (Wen Jing etc., 2009, agricultural engineering technology:Greenhouse production, 6:11-12).However, since artificial light utilizes type plant work The light source of factory is artificial light source, and energy consumption is big, operating cost is high, comprehensive income is low, seriously constrains its further popularization and application. In view of this, on the one hand artificial light is needed to improve the efficiency of light energy utilization of plant using type plant factor, be improved in actual production On the other hand yield then needs the nutritional ingredient and functional materials content (such as Vc) that improve plant, that is, promotes the additional of product Value promotes it to promote and develop to increase the economic benefit that artificial light utilizes type plant factor.
Invention content
Goal of the invention:Present invention aims at provide it is a kind of improve plant factor in leaf vegetables Vc contents method, Current artificial light is improved using the illumination method of type plant factor, using the Later growth in plant from plant bottom The mode for carrying out continuous light filling improves the Vc contents in plant leaf, improves product quality.
Technical solution:The method of the present invention for improving leaf vegetables Vc contents in plant factor, including:
(1) Pregermination and seedling breeding;
(2) vegetable seedling cultivated is transplanted to cultivating on the cultivating stand of plant factor, 6~8 days before culture Top light photograph is carried out, the top light of resynchronisation progress later is shone and the continuous light filling in bottom, bottom light filling harvest after 7~10 days;
Wherein, the direction of illumination that top light is shone is to be irradiated from romaine lettuce top to bottom;When the continuous light filling in bottom, the photograph of illumination It is to be irradiated from romaine lettuce bottom to top to penetrate direction, and intensity of illumination is 40~60 μm of olm-2·s-1, light application time is for 24 hours/day.
In step (1), seeding and seedling raising will be carried out after vegetable seeds vernalization, rearing condition is that intensity of illumination is 340~360 μ mol·m-2·s-1, light application time is 12~14hd-1, temperature is 20~22 DEG C, and relative humidity is 60~70%, CO2Concentration For 380~420 μm of olmol-1
It in step (2), is cultivated in plant factor, the Japanese vegetable garden experimental formula that adoptable nutrient solution prescription is 1/2 times (NO3-N 8me·L-1, PO4-P 2me·L-1, K4meL-1, Ca 4meL-1, Mg 2meL-1, Fe 3ppm, B 0.01 ppm of 0.5ppm, Mn 0.5ppm, Zn 0.05ppm, Cu 0.02ppm, Mo;Asao et al, 2013, Scientia Horticulturae, 164:221-231) it, is ventilated using air pump interval, EC is 1.3~1.6dSm-1, pH is 6.5~6.8.
The intensity of illumination that the top light is shone is 200~300 μm of olm-2·s-1, light application time is 12~16hd-1; Preferably, intensity of illumination is 200 μm of olm-2·s-1, light application time 16hd-1
Preferably, top light photograph was carried out at first 7 days of culture, resynchronisation later carries out top light and continuously mended according to bottom Light, bottom light filling harvest after 7 days;The intensity of illumination of the continuous light filling in bottom is 50 μm of olm-2·s-1
The temperature of the culture is 20~25 DEG C, and relative humidity is 60~65%, CO2A concentration of 800~1000 μm of ol mol-1;Preferably, the temperature of the culture is 20~22 DEG C, CO2A concentration of 1000 μm of olmol-1
The top light is White LED or red LED according to the light source with the continuous light filling in bottom.
The vegetables are romaine lettuce, and kind can be greenery romaine lettuce or Luo Man romaine lettuce.
In the present invention, the blade of whole strain romaine lettuce plant is divided into 6 layers from outer layer to internal layer, wherein from the 1st of outside number Belong to siphonal lobe to the 8th leaf, the 9th to the 18th leaf belongs to internal lobe, and every layer of blade includes 2-3 piece leaves.
When the continuous light filling in bottom, intensity of illumination is measured as apart from 4.0~4.5cm of cultivation plate height i.e. siphonal lobe position.
Compared with prior art, beneficial effects of the present invention are:
1, yield is improved.Harvesting first 1 week progress plant bottom in plant, continuous light filling processing improves siphonal lobe upwards Net Photosynthetic Rate promotes the accumulation of photosynthate, compared with non-light filling planting type, this method make output increased 40% with On.
2, the Vc contents of blade are increased.First 1 week progress plant bottom is harvested using the method for the present invention in plant upwards to connect Continuous light filling processing is, it can be achieved that Vc contents improve 30% or more.
3, standardization, large-scale production are easily achieved.LED is small, calorific value is low, can planar package also can be developed into gently Thin product, therefore can be directly installed on field planting plate, using intelligent control system control light opening and closing, to realize mark Standardization, large-scale production.
4, environmentally protective.Supplementary lighting sources use LED, as a kind of new type light source, with it is energy-efficient, environmentally protective, The advantages that service life is long, therefore there are no pollution to the environment, environmentally protective, safe during entire continuous light filling.
Description of the drawings
Fig. 1 is the schematic diagram using bottom light filling.
Specific implementation mode
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments are merely to illustrate the present invention Rather than limit the scope of the invention, after having read the present invention, various equivalences of the those skilled in the art to the present invention The modification of form falls within the application range as defined in the appended claims.
Embodiment 1
(1) it by greenery romaine lettuce seed presoaking and germinating, is sowed in 300 seedling culture hole plates of the hole containing sponge block, is placed in seedling rearing room Culture, environment temperature are 20~22 DEG C, and relative humidity is 60~70%, CO2A concentration of 380~420 μm of olmol-1, illumination is strong Degree is 340~360 μm of olm-2·s-1, light application time 12hd-1, light source is cold fluorescent lamp.
(2) after nursery 3 weeks, the seedling replanting for selecting growing way consistent utilizes the stereoscopic cultivating frame of type plant factor to artificial light On, environment temperature is 25/20 DEG C (day/night), and relative humidity is 60~70%, CO2A concentration of 800~1000 μm of ol mol-1, intensity of illumination is 200 μm of olm-2·s-1, light application time 16hd-1, light source is White LED.Nutrient solution is 1/2 times Japanese vegetable garden experimental formula nutrient solution, using deep liquid stream cultivation system, nutrient solution circulates, EC be 1.3~1.6dSm-1, pH It is 6.5~6.8.
(3) the 1st week is the laundering period after transplanting, and comes into effect within the 2nd week the continuous light filling processing in bottom and (is uninterruptedly planted by romaine lettuce Strain bottom carries out upward light filling processing), when the light filling of bottom, the lighting condition of crown is constant.Supplementary lighting sources are planted by being fixed on On wall panel (having hole on the plate of cultivation plate, there is sponge block, sponge block to encase the stem of romaine lettuce in hole, cultivation plate is placed on cultivating stand) White LED provides, and intensity of illumination is 50 μm of olm-2·s-1(apart from 4.0~4.5cm of cultivation plate height, that is, siphonal lobe position). Other environmental conditions remain unchanged, and continuous bottom light filling harvests after handling 1 week.In addition, setting contrast groups 1 and contrast groups 2.Comparison Group 1:Bottom light filling processing is come into effect within the 2nd week after transplanting, intensity of illumination is 50 μm of olm-2·s-1, light application time 16h d-1(i.e. with overhead illumination time consistency);Control group 2:Come into effect within the 4th day after transplanting bottom light filling processing, intensity of illumination 50 μmol·m-2·s-1, light application time 16hd-1(i.e. with overhead illumination time consistency, and make this group of light filling handle total hour Number is identical as implementation group);The bottom supplementary lighting sources that contrast groups 1 and 2 use are consistent with implementation group, harvested after transplanting 2 weeks.Examination It tests and is repeated 3 times.
Control group is the non-light filling processing group in bottom, ensures the nursery of control group, implementation group and contrast groups during experiment, moves Cultivation, cultivation, moisture are identical as nutrient management mode.
Compare control group, implementation group and contrast groups greenery romaine lettuce yield and blade Vc contents.As shown in Table 1, it is adopted in romaine lettuce It receives preceding 1 week, giving the continuous white light light filling in bottom and handling can make yield be increased to 220.4g from 152.5g, amplification 44.5%, Vc contents are increased to 40.5mg/100g FW, amplification 31.1% from 30.9mg/100g FW in blade.And after Growth of Lettuce It is 17.6~28.4% that phase, which gives bottom discontinuous light filling processing amplification, and Vc amplification is 4.2~9.4% in blade.
The continuous light filling in 1 White LED bottom of table handles the influence to greenery romaine lettuce yield and Vc contents
1Indicate mean value ± standard error,2Indicate the single factor test significant difference analysis result between same column different disposal:It is different Letter represents significant difference (p=0.05), and same letter indicates that there was no significant difference;Following table is same.
Embodiment 2
(1) it by Luo Man romaine lettuce seed presoaking and germinatings, is sowed in 300 seedling culture hole plates of the hole containing sponge block, is placed in seedling rearing room Culture, environment temperature are 20~22 DEG C, and relative humidity is 60~70%, CO2A concentration of 380~420 μm of olmol-1, illumination is strong Degree is 340~360 μm of olm-2·s-1, light application time 12hd-1, light source is cold fluorescent lamp.
(2) after nursery 3 weeks, the seedling replanting for selecting growing way consistent utilizes the stereoscopic cultivating frame of type plant factor to artificial light On, environment temperature is 25/20 DEG C (day/night), and relative humidity is 60~70%, CO2A concentration of 800~1000 μm of ol mol-1, intensity of illumination is 200 μm of olm-2·s-1, light application time 16hd-1, light source is White LED.Nutrient solution is 1/2 times Japanese vegetable garden experimental formula nutrient solution, using deep liquid stream cultivation system, nutrient solution circulates, EC be 1.3~1.6dSm-1, pH It is 6.5~6.8.
(3) the 1st week is the laundering period after transplanting, and comes into effect within the 2nd week the continuous light filling processing in bottom (i.e. continuously by romaine lettuce plant Bottom carries out upward light filling processing), when the light filling of bottom, the lighting condition of crown is constant.Supplementary lighting sources are by being fixed on cultivation It is red on plate (having hole on the plate of cultivation plate, there is sponge block, sponge block to encase the stem of romaine lettuce in hole, cultivation plate is placed on cultivating stand) Color LED is provided, and intensity of illumination is 50 μm of olm-2·s-1(apart from 4.0~4.5cm of cultivation plate height, that is, siphonal lobe position).Its Its environmental condition remains unchanged, and continuous bottom light filling harvests after handling 1 week.In addition, setting contrast groups 1 and contrast groups 2.Contrast groups 1:Bottom light filling processing is come into effect within the 2nd week after transplanting, intensity of illumination is 50 μm of olm-2·s-1, light application time 16hd-1 (i.e. with overhead illumination time consistency);Control group 2:Bottom light filling processing is come into effect within the 4th day after transplanting, intensity of illumination is 50 μ mol·m-2·s-1, light application time 16hd-1(i.e. with overhead illumination time consistency, and make this group of light filling handle total hour Number is identical as implementation group);The bottom supplementary lighting sources that contrast groups 1 and 2 use are consistent with implementation group, harvested after transplanting 2 weeks.Examination It tests and is repeated 3 times.
Control group is the non-light filling processing group in bottom, ensures the nursery of control group, implementation group and contrast groups during experiment, moves Cultivation, cultivation, moisture are identical as nutrient management mode.
Compare control group, implementation group and contrast groups Luo Man romaine lettuce yield and blade Vc contents are adopted in romaine lettuce as shown in Table 2 It receives preceding 1 week, giving bottom continuous red light light filling and handling can make yield be increased to 231.0g from 161.8g, amplification 42.8%, Vc contents are increased to 43.1mg/100g FW, amplification 33.1% from 32.4mg/100g FW in blade.And after Growth of Lettuce It is 13.2~21.1% that phase, which gives bottom discontinuous light filling processing yield amplification, and Vc amplification is 10.5~11.7% in blade.
The continuous light filling in 2 red LED bottom of table handles the influence to Luo Man romaine lettuce yield and Vc contents

Claims (6)

1. a kind of method improving leaf vegetables Vc contents in plant factor, which is characterized in that including:
(1) Pregermination and seedling breeding;
(2) vegetable seedling cultivated is transplanted to cultivating on the cultivating stand of plant factor, is carried out within 6~8 days before culture Top light is shone, and the top light of resynchronisation progress later is shone and the continuous light filling in bottom, bottom light filling harvest after 7~10 days;
Wherein, the direction of illumination that top light is shone is to be irradiated from romaine lettuce top to bottom;When the continuous light filling in bottom, the irradiation side of illumination To be irradiated from romaine lettuce bottom to top, intensity of illumination is 40~60 μm of olm-2·s-1, light application time is for 24 hours/day.
2. the method according to claim 1 for improving leaf vegetables Vc contents in plant factor, which is characterized in that step (1) in, seeding and seedling raising will be carried out after vegetable seeds vernalization, rearing condition is that intensity of illumination is 340~360 μm of olm-2·s-1, Light application time is 12~14hd-1, temperature is 20~22 DEG C, and relative humidity is 60~70%, CO2A concentration of 380~420 μ mol·mol-1
3. the method according to claim 1 for improving leaf vegetables Vc contents in plant factor, which is characterized in that described The intensity of illumination that top light is shone is 200~300 μm of olm-2·s-1, light application time is 12~16hd-1
4. the method according to claim 1 for improving leaf vegetables Vc contents in plant factor, which is characterized in that described The temperature of culture is 20~25 DEG C, and relative humidity is 60~65%, CO2A concentration of 800~1000 μm of olmol-1
5. the method according to claim 1 for improving leaf vegetables Vc contents in plant factor, which is characterized in that described Top light is White LED or red LED according to the light source with the continuous light filling in bottom.
6. the method according to claim 1 for improving leaf vegetables Vc contents in plant factor, which is characterized in that described Vegetables are romaine lettuce, and kind is greenery romaine lettuce or Luo Man romaine lettuce.
CN201810535391.5A 2018-05-30 2018-05-30 A method of improving leaf vegetables Vc contents in plant factor Pending CN108713485A (en)

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PCT/CN2018/100283 WO2019227680A1 (en) 2018-05-30 2018-08-13 Method for increasing vc content of leafy vegetables in plant factory

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CN111869559A (en) * 2020-08-12 2020-11-03 中国农业科学院都市农业研究所 Method for improving yield of leaf vegetables through light energy distribution
CN113875569A (en) * 2021-09-17 2022-01-04 四维生态科技(杭州)有限公司 Cultivation method of leaf vegetable plants

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Application publication date: 20181030