CN108651186A - The cultural method of romaine lettuce in a kind of plant factor - Google Patents

The cultural method of romaine lettuce in a kind of plant factor Download PDF

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Publication number
CN108651186A
CN108651186A CN201810358445.5A CN201810358445A CN108651186A CN 108651186 A CN108651186 A CN 108651186A CN 201810358445 A CN201810358445 A CN 201810358445A CN 108651186 A CN108651186 A CN 108651186A
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China
Prior art keywords
romaine lettuce
light
illumination
plant factor
cultural method
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Pending
Application number
CN201810358445.5A
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Chinese (zh)
Inventor
张更
颜志明
矢守航
冯英娜
王媛花
王全智
刘叶琼
姜莹
笪潇慧
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Jiangsu Polytechnic College of Agriculture and Forestry
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Jiangsu Polytechnic College of Agriculture and Forestry
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Priority to CN201810358445.5A priority Critical patent/CN108651186A/en
Publication of CN108651186A publication Critical patent/CN108651186A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/04Electric or magnetic or acoustic treatment of plants for promoting growth
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/14Measures for saving energy, e.g. in green houses

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention discloses a kind of cultural methods of romaine lettuce in plant factor, including:Pregermination and seedling breeding;It will be cultivated on the romaine lettuce seedling replanting to the cultivating stand of plant factor cultivated, carry out top light photograph within 14~21 days before culture, carry out bottom light filling later, bottom light filling harvests after 10~15 days;Wherein, the direction of illumination that top light is shone is to be irradiated from romaine lettuce top to bottom;When the light filling of bottom, the direction of illumination of illumination is to be irradiated from romaine lettuce bottom to top, and intensity of illumination is 30~40 μm of olm‑2·s‑1, light application time is 14~16h/ days.The present invention improves existing artificial light cultural method, using by the way of the light filling of plant bottom, improves the luminous environment of romaine lettuce siphonal lobe, improves siphonal lobe Net Photosynthetic Rate, and Delaying Leaf-Senescence process reduces waste leaf loss amount, to increase romaine lettuce yield.

Description

The cultural method of romaine lettuce in a kind of plant factor
Technical field
The invention belongs to industrialized agriculture production fields, and in particular to the cultural method of romaine lettuce in a kind of plant factor.
Background technology
Plant factor is a kind of new and effective facilities horticulture production model, not by or seldom restricted by natural conditions, be Generally acknowledged industrialized agriculture superlative degree developing stage in the world.It passes through illumination in intelligence control system control facility, temperature, wet Degree, CO2The environmental elements such as concentration and nutrient solution create suitable condition for growth and development of plants, and efficient production are combined to count It draws, and realization crop anniversary continuous production (Liu Wenke etc., 2014, illuminating engineering journal, 25 (4):50–53).
Plant factor can be divided into artificial light type plant factor, sun light type plant factor and too according to illumination source Sunlight and artificial light and with three kinds of type plant factor.Artificial light type plant factor is mainly used for the shorter leaf vegetables vegetable of growth cycle The production of dish, wherein romaine lettuce are one of Main Cultivation vegetables.Layer stereo high-density planting is used in artificial light type plant factor Pattern improves land utilization ratio, increases yield, but high planting density also brings some drawbacks, in influences of plant crown lower part Outer foil causes illumination insufficient due to being blocked by top vane and surrounding plant, it may appear that photosynthetic rate reduction, blade The phenomenon that aging shifts to an earlier date.The phenomenon not only seriously affects romaine lettuce yield, but also needs to remove aging siphonal lobe before listing, and also increases The input of manpower and time are added.How to improve romaine lettuce plant siphonal lobe luminous environment, delay siphonal lobe aging, reduces waste leaf loss amount, It is the active demand of vast production grower.The solution of the problem can improve romaine lettuce yield, and then increase artificial light type plant work The output capacity and economic benefit of factory push the popularization and development of artificial light type plant factor.
Invention content
Goal of the invention:Present invention aims at the cultural method for providing romaine lettuce in a kind of plant factor, to existing artificial Light cultural method is improved, and using by the way of the light filling of plant bottom, improves the luminous environment of romaine lettuce siphonal lobe, it is net to improve siphonal lobe Photosynthetic rate, Delaying Leaf-Senescence process reduce waste leaf loss amount, to increase romaine lettuce yield.
Technical solution:The cultural method of romaine lettuce in plant factor of the present invention, including:
(1) Pregermination and seedling breeding;
(2) it will be cultivated on the romaine lettuce seedling replanting to the cultivating stand of plant factor cultivated, 14~21 before culture It carries out top light photograph, and the top light of resynchronisation progress later is shone and bottom light filling, bottom light filling harvest after 10~17 days;
Wherein, the direction of illumination that top light is shone is to be irradiated from romaine lettuce top to bottom;When the light filling of bottom, the irradiation side of illumination To be irradiated from romaine lettuce bottom to top, intensity of illumination is 30~40 μm of olm-2·s-1, light application time is 12~16h/ days.
In step (1), seeding and seedling raising will be carried out after romaine lettuce presprouting of seeds, rearing condition is that intensity of illumination is 340~350 μ mol·m-2·s-1, light application time is 12~14hd-1, temperature is 20~22 DEG C, and relative humidity is 60~65%, CO2Concentration For 400~500 μm of olmol-1;It is further preferred that light application time is 12hd-1, temperature is 20 DEG C, CO2A concentration of 400~ 420μmol·mol-1
It in step (2), is cultivated in plant factor, the Japanese vegetable garden experimental formula that adoptable nutrient solution prescription is 1/2 times (NO3-N 8me·L-1, PO4-P 2me·L-1, K 4meL-1, Ca 4meL-1, Mg 2meL-1, Fe 3ppm, B 0.5ppm, Mn 0.5ppm, Zn 0.05ppm, Cu 0.02ppm, Mo 0.01ppm;Asao et al, 2013, Scientia Horticulturae, 164:221-231) it, is ventilated using air pump interval, EC is 1.3~1.6dSm-1, pH is 6.5~6.8.
The intensity of illumination that the top light is shone is 200~300 μm of olm-2·s-1, light application time is 12~16hd-1; Preferably, intensity of illumination is 200 μm of olm-2·s-1, light application time 16hd-1
The temperature of the culture is 20~25 DEG C, and relative humidity is 60~65%, CO2A concentration of 800~1000 μm of ol mol-1;Preferably, the temperature of the culture is 20~22 DEG C, CO2A concentration of 1000 μm of olmol-1
In the present invention, the blade of whole strain romaine lettuce plant is divided into 6 layers from outer layer to internal layer, wherein from the 1st of outside number Belong to siphonal lobe to the 8th leaf, the 9th to the 18th leaf belongs to internal lobe, and every layer of blade includes 2-3 piece leaves.
Compared with prior art, beneficial effects of the present invention are:
1, waste leaf loss late is reduced, commodity leaf-making quantity is improved.Using the method for the present invention plant is carried out in the Growth of Lettuce later stage The upward light filling in bottom can improve the Net Photosynthetic Rate of siphonal lobe, effectively delay outer senescence of leaves, reduce waste leaf loss late 5.7%, Improve yield 17.9%.
2, scale, standardized production are easily achieved.It, can be direct since LED is light, small, easy to install, fever is few On the field planting plate of romaine lettuce in cultivation system, according to production grower's demand, light is automatically adjusted using intelligent control system The open and close in source, to realize scale, standardized production.
3, environmentally protective.The LED of use has many advantages, such as efficient, energy-saving and environmental protection, long lifespan, and entire light filling process is to ring It does not pollute, is safe in border.
4, bottom light filling is carried out with top light according to synchronous, first, for the ease of management, second is that due to artificial light type plant work The principal light source of factory is artificial light source, and energy consumption and economic input are larger, but it uses and is completely closed form production, not certainly by the external world The influence of right photoperiod, therefore can select to open illumination (the idle electricity consumption period electricity charge are cheap) in the idle electricity consumption period, it is maximum Limit reduces electric cost, and then increases economic benefit.
Description of the drawings
Fig. 1 a are the schematic diagram using bottom light filling;
Fig. 1 b are to inhibit siphonal lobe ageing process by bottom light filling;
Fig. 2 is bottom light filling on the photosynthetic influence of siphonal lobe;
Fig. 3 is influence of the bottom light filling to siphonal lobe waste leaf rate and yield (commodity leaf).
Specific implementation mode
With reference to specific embodiment, the present invention is furture elucidated, it should be understood that these embodiments are merely to illustrate the present invention Rather than limit the scope of the invention, after having read the present invention, various equivalences of the those skilled in the art to the present invention The modification of form falls within the application range as defined in the appended claims.
Embodiment 1
(1) it by Luo Man romaine lettuce seed presoaking and germinatings, is sowed in 300 hole trays of the hole containing sponge block, the nursery under fluorescent lamp is educated Slender part is that intensity of illumination is 340~350 μm of olm-2·s-1, light application time 12hd-1, temperature is 20 DEG C, relative humidity For 60~65%, CO2A concentration of 400~420 μm of olmol-1
After (2) 3 weeks, by second true leaf is fully deployed, growing way is consistent seedling replanting to artificial light type plant factor On stereoscopic cultivating frame, it is 200 μm of olm that light source, which uses White LED, intensity of illumination,-2·s-1, light application time 16hd-1, temperature Degree is 20~22 DEG C, and relative humidity is 60~65%, CO2A concentration of 1000 μm of olmol-1.Cultural method is planted using deep liquid stream Training system is ventilated with air pump interval, and the Japanese vegetable garden experimental formula that nutrient solution prescription is 1/2 times, control EC is 1.3~1.6dSm-1, PH is 6.5~6.8.
(3) start within the 3rd week after transplanting, carry out upward light filling processing in romaine lettuce plant bottom, intensity of illumination is 40 μm of ol m-2·s-1, light application time 16hd-1(synchronous with plant overhead illumination to carry out), wherein the light source of light filling processing is by white LED light plate provides, and White LED tabula rasa, which is fixed on cultivation plate, (to be had hole, there is sponge block, sponge block packet in hole on the plate of cultivation plate Firmly the stem of romaine lettuce, cultivation plate are placed on cultivating stand) on, it can be from the upward light filling in plant bottom, in 3.5~4.5cm height (siphonal lobe position) light intensity is 40 μm of olm-2·s-1.Plant bottom light filling harvests after 16 days.
Control group is non-light filling processing group, nursery, transplanting, cultivation, moisture and nutrient management mode and implementation group phase Together.
Compare control group (non-light filling) and implementation group (bottom light filling) Luo Man Growth of Lettuce development index and yield.
When harvest, the blade of whole strain plant is divided into 6 layers from outer layer to internal layer, wherein from the 1st to the 8th of outside number Leaf belongs to siphonal lobe, and the 9th to the 18th leaf belongs to internal lobe, and every layer of blade includes 2-3 piece leaves.Measure whole fresh weights of romaine lettuce (TFW;Total fresh weight), it is removed according to the postharvest handling standard of Mirai companies of factory of Japanese plant external weathering Siphonal lobe (SLW:Senescent leaf weight) obtain commodity leaf weight (MFW;Marketable fresh weight), MFW=TFW-SLW;Waste leaf rate (wastage)=SLW/TFW.In harvesting in first 3 days, using Li-6400 photosynthesis measurements Instrument measure the 3rd layer of blade in siphonal lobe photosynthetic rate (Yamori et al, Plant Cell&Environment, 2005,28 (4):536–547).Meanwhile the PSII maximum light of blade is measured using imaging-PAM system Imaging-PAM (Germany) Chemical efficiency (Fv/Fm), specific operating method is as follows:Beaten respectively from 6 layers of blade main lobe arteries and veins both sides of plant take it is a diameter of The leaf disk of 1.3cm carries out vacuum infiltration (Shao et al, Pakistan using the deionized water containing 0.005% polysorbas20 Journal of Botany, 2013,45:1905-1912), F is measured after dark adaptation 30minv/Fm
Concrete outcome is shown in Fig. 1-3, gives bottom light filling processing in 16 days and can effectively delay siphonal lobe aging, improves siphonal lobe Net Photosynthetic Rate can make waste leaf loss late reduce by 5.7%, and then improve 15% or more yield.

Claims (6)

1. the cultural method of romaine lettuce in a kind of plant factor, which is characterized in that including:
(1) Pregermination and seedling breeding;
(2) will be cultivated on the romaine lettuce seedling replanting to the cultivating stand of plant factor cultivated, before culture 14~21 days into Row top light is shone, and the top light of resynchronisation progress later is shone and bottom light filling, bottom light filling harvest after 10~17 days;
Wherein, the direction of illumination that top light is shone is to be irradiated from romaine lettuce top to bottom;When the light filling of bottom, the direction of illumination of illumination is It is irradiated from romaine lettuce bottom to top, intensity of illumination is 30~40 μm of olm-2·s-1, light application time is 12~16h/ days.
2. the cultural method of romaine lettuce in plant factor according to claim 1, which is characterized in that top light is shone and bottom is mended The light source of light is White LED.
3. the cultural method of romaine lettuce in plant factor according to claim 1, which is characterized in that in step (1), by romaine lettuce Seeding and seedling raising is carried out after presprouting of seeds, rearing condition is:Intensity of illumination is 340~350 μm of olm-2·s-1, light application time is 12~14hd-1, temperature is 20~22 DEG C, and relative humidity is 60~65%, CO2A concentration of 400~500 μm of olmol-1
4. the cultural method of romaine lettuce in plant factor according to claim 1, which is characterized in that in step (2), the top The intensity of illumination of portion's illumination is 200~300 μm of olm-2·s-1, light application time is 12~16hd-1
5. the cultural method of romaine lettuce in plant factor according to claim 1, which is characterized in that in step (2), the training Foster temperature is 20~25 DEG C.
6. the cultural method of romaine lettuce in plant factor according to claim 1, which is characterized in that in step (2), the training Foster relative humidity is 60~65%, CO2A concentration of 800~1000 μm of olmol-1
CN201810358445.5A 2018-04-20 2018-04-20 The cultural method of romaine lettuce in a kind of plant factor Pending CN108651186A (en)

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Application Number Priority Date Filing Date Title
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111869559A (en) * 2020-08-12 2020-11-03 中国农业科学院都市农业研究所 Method for improving yield of leaf vegetables through light energy distribution
CN111919691A (en) * 2020-07-29 2020-11-13 福建省中科生物股份有限公司 Method for culturing ginseng seedlings in plant factory
CN113873727A (en) * 2021-09-27 2021-12-31 佛山电器照明股份有限公司 Method, equipment and system for controlling under-tree illumination
CN114402924A (en) * 2022-01-20 2022-04-29 中国农业科学院都市农业研究所 Method for shortening whole growth period of lettuce by using LED illumination
CN114451284A (en) * 2022-01-28 2022-05-10 正恒(上海)农业科技有限公司 Planting, harvesting and packaging method for living root lettuce in plant factory

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GENG ZHANG等: "Supplemental Upward Lighting from Underneath to Obtain Higher Marketable Lettuce (Lactuca sativa) Leaf Fresh Weight by Retarding Senescence of Outer Leaves", 《FRONTIERS IN PLANT SCIENCE》 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111919691A (en) * 2020-07-29 2020-11-13 福建省中科生物股份有限公司 Method for culturing ginseng seedlings in plant factory
CN111869559A (en) * 2020-08-12 2020-11-03 中国农业科学院都市农业研究所 Method for improving yield of leaf vegetables through light energy distribution
CN113873727A (en) * 2021-09-27 2021-12-31 佛山电器照明股份有限公司 Method, equipment and system for controlling under-tree illumination
CN113873727B (en) * 2021-09-27 2023-09-12 佛山电器照明股份有限公司 Method, equipment and system for controlling illumination under tree
CN114402924A (en) * 2022-01-20 2022-04-29 中国农业科学院都市农业研究所 Method for shortening whole growth period of lettuce by using LED illumination
CN114451284A (en) * 2022-01-28 2022-05-10 正恒(上海)农业科技有限公司 Planting, harvesting and packaging method for living root lettuce in plant factory
CN114451284B (en) * 2022-01-28 2023-08-18 正恒(上海)农业科技有限公司 Planting, harvesting and packaging method for living lettuce with root in plant factory

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Application publication date: 20181016